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It is obvious from Figure 3 that cellulase production increased with increasin g temperature from 30.
Bacterial cellulases are constitutively produced by submerged fer- mentation (SmF) in industries
employing mainly geneti- cally modified strains. P-glucosidase activity can also be induced by
elaborately controlling culture conditions. ABSTRACT This study evaluates the influence of growth
parameters such as pH, temperature, Carboxy Methyl Cellulose (CMC) concentration and agitation
on cellulase production from three bacterial strains, viz., Achromobacter xylosoxidans BSS4,
Bacillus sp. We use cookies on our website to ensure you get the best experience. To test the
evolutionary relationships, phylogenetic analysis was performed with the program MEGA 6.0 using
the 16SrRNAsequence. BSS2 Creamy, opaque, mucoidal colonies, circular and convex Bacillus sp.
We tried CMC as supplement at different concentrations, i.e., 0.5%, 1% and 1.5%. For
Pseudomonas sp. and Bacillus sp. optimum CMC concentration was 1%, while A. As a result,
isolation and characterization of cellulase-pro ducing bac- teria will continue to be a principal
component of enzy- me research. Partly Is the study design appropriate and is the work technically
sound. You are an Editor for the journal in which the article is published. The Figure 33 shows the
reusability of the immobilized enzyme for 8 times. Production of cellulases by fungal species
cultivated on solid-state fermentation of untreated banana pseudostem as a substrate.
YogeshIJTSRD 2019 bt 204 module 1(part d) 2019 bt 204 module 1(part d) Leon Ittiachen
Quantitative analysis of total phenolic content in avocado (persia americana). The funders had no role
in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
The possible explanation for the alkaline effect was that the inhibitory compounds that are generated
during substrate pre-treatment have a deleterious effect on microbial growth and enzyme production.
Results and discussion Bacterial identification The SR60 isolate was revealed to be a Gram-positive
spore-forming bacillus, facultative anaerobe, catalase-positive; it was negative for indole, H 2 S
production and citrate utilization bacterium ( Table 1 ). Titer improvement by the semi-batch process
was however dependent on the types of microorganisms used. Application for Denim Biostoning
and Cellulosic Nanofiber. These products can be used as thermostable enzymes in the production of
biofuels in crucial stages of this bioprocess. Varying concentrations of potato starch from 1% to 5%
were then tested Maximum cellulase activity was seen with 5% potato starch. Hence, 150 rpm was
found suitable for the isolates described herein for better pro- duction of cellulase. Such activity
levels could feasibly be practically applied to the development of scaled-up processes. Close READ
LESS CITE CITE HOW TO CITE THIS REPORT Olmos-Soto J. Note: To add your institution or
organisation, start typing the name and then select the correct name from the list. Cellulase
production appears to depend on pH value. Production of the Cellulase Enzyme System by Locally
Isolated Trichoderma and Aspergillus Species Cultivated on Banana Pseudostem during Solid-State
Fermentation. The enzyme activities of cellulase (FPase), endoglucanse (CMCase), and ?-glucosidase
are shown in Table 1. The pre-treatment of BPS prior to SSF did not improve the overall production
of the cellulase enzyme system as determined by FPase activity, but it enhanced the specific type of
enzyme(s) by a particular fungal strain(s). Phylogenetic tree of isolated SR60 and other related
species based on 16S rRNA sequences.
International Journal of Turbomachinery, Propulsion and Power (IJTPP). Ja’afaru MI: Screening of
fungi isolated from environmental samples for xylanase and cellulase production. Sophorose, a
decomposition product of crystalline cellulose, is thought to be an indispensable inducer of cellulase
activity. PC-3-7 was created as a strain, capable of partially producing cellulase enzymes. C, whereas
Bacillus sp. BSS3 and A. xylosoxidans BSS4 showed maximum production at pH 9 and 7,
respectively (6th h of incubation at 37 ? C). (c) Effect of sub- strate concentration on cel lulase
production from Pseudomonas sp. The production of the cellulase enzyme system by A. However,
protease and amylase activity cannot be considered as halotolerant because these enzymes only have
good activity at 0.25-0.5 M. In this sense, I believe the conclusion must be reoriented to point out
these results. The results obtained in this study showed that the production of the cellulase enzyme
system in SSF of untreated BPS was strongly influenced by the moisture content of the substrate.
Pseudo- monas sp. BSS2 is Gram ? ve, motile rod without en- dospores; Ba cillus sp. Evaluation of
cellulolytic capacity of the fungal isolates. In conclusion, we obtained three strains, viz., Pseudo-
monas sp., Bacillus sp. and A. xylosoxidans with cellu- lolytic activity, which more promising than
the reported cultures of related genera. Conclusions: Bacillus subtilis SR60 was shown in this study
be capable of producing protease, cellulase, amylase and xylanase when submitted to a high salinity
environment. The optimization of process parameters for cellulase production by each culture in
cluded effects of pH, tem- perature, CMC concentration and agitation. Note that from the first issue
of 2016, this journal uses article numbers instead of page numbers. Three grams (3 g) of untreated
BPS was inoculated as indicated above for all fungal species. We use cookies on our website to
ensure you get the best experience. An increased level of ?-glucosidase in the co-culture of A. The
work may be protected under the copyright laws of other jurisdictions when used in those
jurisdictions. Pre-treatment of the BPS substrate did not improve the overall production of the
cellulase enzymes for T. The activity was measured against a reagent blank at 540 nm in a UV-Vis
spectrophotometer (Shimadzu, Japan). The magnetic nanoparticles dissolved in HCL (0.1M) were
stored. The organism was obtained from National Collection of Industrial Microorganisms (NCIM),
Pune. IOSR Journals Blue Team MCDB 150AL-Poster Presentation (2) Blue Team MCDB 150AL-
Poster Presentation (2) Hung Dang More Related Content Viewers also liked Transcriptional analysis
of Trichoderma reesei under conditions inducing cell. When criticisms of the article are based on
unpublished data, the data should be made available. In general, the temperature had a direct
influence on the production of the cellulase enzyme system by all fungal strains. By using our site,
you agree to our collection of information through the use of cookies. This might be because pre-
treatment might have resulted in the partial hydrolysis of the polysaccharides in the BPS, releasing
some soluble oligosaccharides or other disaccharides other than cellobiose, which act as inducers, or
through transglycosylation had induced ?-glucosidases and repressed endoglucanase and
cellobiohydrolases. Microorganisms of the genera Trichoderma and Aspergillus are thought to be
cellulase producers, and crude enzymes produced by these microorganisms are commercially
available for agricultural use. The effect of initial moisture content of banana pseudostem on the
production of the cellulase enzyme system during solid-state fermentation of untreated banana
pseudostem. ( a ) T. longibrachiatum LMLSAUL 14-1, ( b ) T. However, protease and amylase
activity cannot be considered as halotolerant because these enzymes only have good activity at 0.25-
0.5 M. In this sense, I believe the conclusion must be reoriented to point out these results.
Production of cellulases by fungal species cultivated on solid-state fermentation of untreated banana
pseudostem as a substrate. Production of the Cellulase Enzyme System by Locally Isolated
Trichoderma and Aspergillus Species Cultivated on Banana Pseudostem during Solid-State
Fermentation. BSS2, Bacillus sp. BSS3 and A. xylosoxidans BSS4 in BSM supplemented with
0.5%, 1% and 1.5% CMC. Pseudomonas sp. BSS2 and Bacillus sp. If you do not have access to your
original account, please contact us. Comparative analysis of structural variations due to genome
shuffling of Bacillus Subtilis VS15 for improved cellulase production. In general, the co-cultivation
of the above fungi did not yield an improvement in the production of the cellulase enzyme system.
Quantitative analysis of total phenolic content in avocado (persia americana). SSF offers the
advantage of utilizing agro-industrial waste as substrate support. An evaluation of carbon source
utilization by these microorganisms, suggested that the production of high-titer cellulases
necessitated an increase in the concentration of Avicel, the carbon source. The amplified 16S rRNA
PCR product of 1500bp was sequenced and the unknown organism was identified using the
maximum aligned 16SrRNA sequences available in the GenBank of NCBI through BLAST search.
IRJET Journal Welcome to International Journal of Engineering Research and Development (IJERD)
Welcome to International Journal of Engineering Research and Development (IJERD) IJERD Editor
Effect of Different Drying Methods on Chemical Composition of Unripe Plantain. PRODUCTION
OF CELLULASE ENZYME FROM Aspergillus terreus. Production of the Cellulase Enzyme
System by Locally Isolated Trichoderma and Aspergillus Species Cultivated on Banana Pseudostem
during Solid-State Fermentation. Various industries like textiles, paper, agriculture and biofuel need
enzymatic treatment (cellulase) which costs up to the total cost production of 40% to convert into the
useful product. Transcriptional analysis of Trichoderma reesei under conditions inducing cell. Bailey
MJ, Biely P, Poutanen K: Interlaboratory testing of methods for assay of xylanase activity.
Trichoderma and Aspergillus species were used to produce cellulases through solid-state fermentation
(SSF) utilizing banana pseudostem (BPS) as a carbon source. The bacterial cultures were also
characterized based on colony nature, shape of colo ny margine, size, transpar- ency, color, shape and
elevation ( Table 1 ). The isolates were then evaluated by submerged fermentation process for
maximum cellulase production. BSS3 showed maximum cellulase production with 1% CMC on 12th
h and 6th h at pH 8 and 9, respectively at 37. Effect of different CMC concentrations on cellulase
production was evaluated, after fixing the optimum pH and temperature for each culture.
Optimization of Cultural Parameters for Cellulase Enzyme Production from Fung. Cellulolytic
enzymes comprise a group of glycosidic hydrolases, including endoglucanases, exoglucanases and
beta-glycosidase. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Olmos-Soto J.
The graph in Figure 21 predicts the peptide peaks having different. On the basis of these findings, a
scale-up experiment was conducted in a 1-kL tank using the semi-batch culturing method with 10%
Avicel as a carbon source and soybean curd as a nitrogen source. Total of thirteen bacterial isolates
found to be positive on screening media. In conclusion, we obtained three strains, viz., Pseudo-
monas sp., Bacillus sp. and A. xylosoxidans with cellu- lolytic activity, which more promising than
the reported cultures of related genera. So we have selected wood-yards on Kallai river— famous for
numerous timber based industries—as our site for sample collection. Effect of temperature on the
enzyme production was (b) (a) (c) Figure 2.
The optimum PH was found to be 7 for the three samples. The magnetic nanoparticles dissolved in
HCL (0.1M) were stored. The ?-glucosidase was the highest during SSF on 3% NaOH BPS. The
information that you give us will be displayed next to your comment. Effect of temperature on the
enzyme production was (b) (a) (c) Figure 2. In general, pre-treatment of different agricultural waste
products including sugarcane bagasse, cassava bagasse, wheat bran, and rice straw improved enzyme
production more than the untreated substrates. The iodine test (plate assay) for screening of cellulase
activity was performed on BSM with CMC agar, in which all the three cultures showed well-defined
clear zone around the colony, which indicates that the CMC in the clear zone area was hydrolyzed by
the cellulase produced by the bacteria. Similarly, endoglucanse activity decreased during SSF on the
pre-treated substrate. Results and discussion Bacterial identification The SR60 isolate was revealed
to be a Gram-positive spore-forming bacillus, facultative anaerobe, catalase-positive; it was negative
for indole, H 2 S production and citrate utilization bacterium ( Table 1 ). BSS3 showed maximum
enzyme yield which can be explored for further scale up studies with an industrial perspective.
Carboxymethyl cellulase production optimization from Glutamicibacter arilaitensis strain ALA4 and
its application in lignocellulosic waste biomass saccharification. The work may be protected under
the copyright laws of other jurisdictions when used in those jurisdictions. The banana plant is a
herbaceous plant of the genus Musa and cultivated for mainly fruit production. Veras B: Screening
of protease, cellulase, amylase and xylanase from the salt-tolerant and thermostable marine Bacillus
subtilis strain SR60. Production of Cellulase from Aspergillus Isolates and It’s. Journal of
Pharmaceutical and BioTech Industry (JPBI). For this study, bacteria microorganisms were isolated
on CMC containing media. This study aims to produce cellulase using Trichoderma reesei and to
characterize the same. The antagonistic behavior of Trichoderma towards the A. I accept the User
Comment Terms and Conditions Please confirm that you accept the User Comment Terms and
Conditions. Isolation and characterization of coprophilous cellulolytic fungi from asian. Download
Export To Sciwheel Bibtex EndNote ProCite Ref. The spore suspensions were shaken vigorously to
dislodge the spores. All the three cultures showed maximum enzyme production at 150 rpm in
combination with already fixed respective pH, temperature and CMC concentrations. No history of
similar work has been mentioned in this regard. Please see my related article about effect of salt on
enzymes 1. From the Figure 38 it was found that the free enzyme was stable at pH 7, 9 and 10. The
BPS was washed to remove soil and other debris using tap water. Generally, SSF of untreated BPS
maintained a high production of cellulase (FPase) in all the fungi evaluated. This semi-batch process
was further utilized in order to optimize the stirring rate, aeration volume, feed change, seed volume,
and the seed culture period. T. reesei generally exhibits poor (3-glucosidase activity.