WAM - Wash Aspitation Monitoring PM - Pressure Monitoring

GOOD GOOD
- Horizontally aligned Deltas - Grouped points, following
- Three separate lines, one a horizontal trend
for each probe - No points off the main axis

BAD BAD
- Aspiration with dispersed - Start with decreasing
Deltas graph then increases the
- Values ranging from 2,633 value and stabilizes (PM
° C to 7,877 ° C sensor exchange)
At the beginning there is a
pressure monitor failure

Root cause: clogs, malfunctioning valves, dirt in the system Root cause: Damaged pressure monitor sensor, probe obstructed
Tips Tips
BAD IN ONE WASH ZONE BAD IN TWO WASH ZONE - Check the pressure monitor sensor and fittings
- Check Probe, thermistor, vacuum - Check vacuum accumulator, tubing, - Check the probe for obstruction
vessel, vacuum valves and drain vacuum valves and drain, vacuum
pump

LLS - Liquid Level Sense M&D 2055 - WZ Valve Pressure Check

GOOD GOOD
- Graphic with aspiration in - Maximum pressure <20 PSI
a continuous line - Slope <= 1.2
- Grouped Points - The three nozzles dispens-
ers has the same behavior,
no significant differences
betwen peak pressure values

BAD BAD
- Aspiration points off the - Gap between the three
main line dispensers
- Amplitude difference - Significant differences
between points between peak pressure
values

Root cause: dirty and damaged antennas, cables poor connection, probe clip, electro- Root cause: Malfunction of the buffer pump or bad adjustment between the two
nic noise sources heads, damaged wash zone valves
Tips Tips
Check for changes in the kit S/N for the same lot of reagent - If pressure <6 PSI check the pump, PM board or leaks
- Observe the graphs of R1 and R2 antennas which they aspire lower volume - Important: Do NOT swap trigger or pre-trigger valves with the wash zone, the
- Low values = Reagent or full test tube, high values = empty container chemical properties can contaminate and damage the system
- Special attention for pipetting order of tests and graphic scale
 Results - Read Profiler Results - Dark Count Distribution
GOOD GOOD
- Reaction starts from zero - Grouped values, following
- Peak reaction between the a pattern
4 th and 6 th sub-reading - Few fluctuation of points
reaction drastically lowered
between 12 th and 14th,
from 15th tends to zero

BAD BAD
- Do not start from zero - Values initiated <50 and
- It has two peaks between drastically increased up to
the 4th and 8th sub- 225
readings

Root cause: Trigger valve, optical system, electrical noise interference Root cause: Light interferences, trigger valve, optical system
Tips Tips

- Check electrical grounding - Acceptable values <100, nominal value <50 as a safety zone
- Check the trigger dispensing valves and optical system -Clean the trigger / pre-trigger manifould and optic area
- Clean the trigger and pre-trigger manifold and optical area - Check the History log for RV loader issues

Results - Dark Count Results - Tail Analysis

GOOD GOOD
- Dark count readings <10 - Consistent values
- The whole population has
value <30%

BAD BAD
- Dark count readings > 10 - Values> 30%
- Errors 1007 and 1008

Root cause: Statics, noise comming from the bad Vortexer and motors grounding ,
Root cause: Trigger valve, light interference, optical system
PT Valves
Tips Tips

- Check the ground conditions - Assays with low RLU signal are more sensitive
-Clean optic area - Value should be <30% (sum of 15 ° to 30 ° sub-read / Signal Count)
- Different assays have different percentage values, however a specific test must
have the same percentage, independent of the reagent lot.
- View the Result.Log graphs to check the Read Profiler