Estd.

2003

St John Para Medical Institute

(Affiliated) To Para Medical Board of India, New Delhi)

:Campus: K. S Saket P. G college Ayodhya faizabad

SYLLABUS
DMLT

Provided by

PARA MEDICAL BOARD OF INDIA

NEW DELHI
 INTRODUCTION
Medical Laboratory Technology
“The Science is devolving different branches of specialization and Medical Sciences are closely linked
with each other scientific Medicine has been nurtured and grown to the present form in the laboratory. It is the
knowledge gained in the technology that makes diagnosis of disease feasible, their treatment and subsequent
follow us success.
Sometime it can harm the patient seriously; mainly the diagnosis depends upon the report of investigation
done in the Laboratory by the Medical laboratory technology. Thus the Laboratory Technician plays a vital role
in the Medicine field .It is difficult for the doctor alone.
In Fact it is necessary that every department in General Hospital Hospital Primary health centre at Taluk
level, every Hospital belonging to state / Centre Govt. and all clinics & Nursing Homes & Practitioners should
have the assistance of trained technician.
So A gap has been developed between the requirement and the availability of trained lab Technician due
to the fast grow in Laboratory & X- Ray field.
To fulfil the gap and to make the Para medical Board of India has realized the problems and start training
course in Medical laboratory technology & X-ray E.CG Technician.

DMLT-FIRST YEAR

COURSE SUBJECTS (MAX. MARKS)

CODE THEORY PRACTICAL

D-2101 ANATOMY & PHYSIOLOGY 100 50

D-2102 MIROBIOLOGY & PARASITOLOGY 100 50

D-2103 HAEMATOLOGY & BLOOD BANKING. 100 50

D-2104 BASIC TECHNOLOGY & ETHICS 100 50

D-2105 HISTO-TECHNOLOGY 100 50

D-2106 BIOCHMISTRY 100 50

DMLT - SECOND YEAR

COURSE SUBJECTS (MAX. MARKS)

THEORY PRACTICAL
CODE
D-2201 BIOCHEMISTRY & CLINICAL PATHOLOGY. 100 50
D-2202 HISTOPATHOLOGY & CYTOLOGY 100 50
D-2203 MICRO,VIRO,MYCO & ADV SEROLOGY 100 50
D-2204 COAGULATION & TRANFUSION MEDICINE 100 50
D-2205 IMMUNOLOGY,HAEMATOLOGY & 100 50
TRANFUSION MEDICINE
 PAPER -1
ANATOMY & PHYSIOLOGY (2101)
Anatomy (Theory):-
1. Introduction:-
(a). Common Anatomical terms & Anatomical Positions .Different parts of the human body
(b) Tissue with Function & Classification (c) Cell & Animal Cell(2.) Skeletal system: (a)
Bones, joint, & Movement (b) Muscles(3) Genito- Urinary System:(a) Male & Female
Reproductive Organic System (b) Urinary bladder, Kidney and Ureter (C). Uterus & Urethra
(4)Respiratory System(a) Lungs & Thoracic Cavity(b) Pleura (c) Surface marking of lungs
(5)Gastro- Intestinal System :-(a) Mouth (b)Pharynx & Salivary gland and Tonsils
(c)Oesophagus &stomach(d) Spleen & Pancreas (e) Gall Bladder & Liver (f) Surface making of
Abdomen (g) Structure of Digestive Tract
(6) Movement of the body(a) Upper Limb –Bones, Important Vessels (b) Lower Limb –Bones
Important Vessels
(7) Nerves System(a) C.S.F & Spinal Card (b) Nerves & Brain(c) SympatheticAnd Sympathetic (d)
Cranial and Spinal Nerves
(8) Cardio –Vascular System(a) Arterial System (b) Lymphatic and Venous System (c) Heart (d)
Surface Making, Important Blood Vessels & Muscles(e) Pericardium

Physiology (Theory)

1. Digestive System(a) Mastication deglutition(b)Function and Composition Saliva (c) Function of

Stomach (d) Function and Composition of gastric juice (e) Function of Pancreatic Juice (f)
Function of Bile
(2) Respiratory System(a) Define-Respiratory Rate(b) Vital Capacity, Cyanosis (c)External &
Internal Respiration (d) Transport of O2 and CO2 in the Blood (e) Function of Respiration its structure
(3) Blood(a) Function of Blood (b) Composition of Blood (c) Anti-Coagulants(c)Description of Blood
Cells(e) Blood Group of A B C O and Rh Factor(f) Function of Lymph (g)anaemia and its Type
(4) Cardio- Vascular System(a) Define of Cardio output(b) Define the blood pressure,
Electrocardiogram (e) Circulation (Systematic and Pulmonary) (f) Function of Heart (g) Function of
Blood vessels (h) Cardio Cycle
(5) Excretory System(a) Kidney (Function)(b) Formation of Urine (Normal and abnormal)(c)
Composition of Urine
(6) ENDOCRINE GLAND(a) Define- Name and hormones Secreted by than (b) Action of
Hormones in our body
(7) Reproductive System(a)Male female Genital System(b) Function of Ovary(c) Formation of Ova
and Their action of ovarian Hormones(d) Function of Testis- Their action of Testosterone(e)Mensuration
Cycle and Fertilization (f)Progesterone and Oestrogen Hormones
(8) Skin(a) Define the Skin (b) Function of Skin
(9)Formation, Function &Composition of C.S.F
(10) Special Senses-Smell, Taste, Touch, Hearing
 Paper - 2
MICROBIOLOGY & PARASITLOGY:-(2102)
MICROBIOLOGY (Theory)
Microbiology is the branch of science that deals with study of Virus, Bacteria and Fungi which cannot be seen
through naked eye.
Morphology of Bacteria
1. Structure & Growth of Bacteria
2. 2..Classification of Bacteria
3. Nutrition of Bacteria
4. Staining of Bacteria
(a) Gram stain, Negative Stain, Ziehl – Neelsen, Albert, Spore Stain.
(b) Composition and preparation of staining Reagents and their composition.
5. Gram Negative Cocci
(a) MeningoCocci&GonoCocci
6. Gram positive – Cocci
(a)staphyloCocci(b)StreptoCocci(c )PneumoCocci
7. Gram Bacilli
(a) Salmonella (b) E-coli
(c) Pseudomonas (d)Shigella
(e) Klebsiellla (f)Haemophilus
8. Gram Positive Bacilli
(a) Anaerobic Bacilli – Clostridia
(b) Areobic – Mycobacterium Tuberculosis and Myeobacteriumleprae. And Corynebacterium
diphtheria.
9. Bacterial Metabolsim :
(a) Requirement of Bactria ,(b)Aerobic (c)Anaerobic(d) Growth
10. Morphology of Fungi :
(a) Cultivation of Pathogenic Fungi (b) Gandida (C) Dermatophytes (d) Asperigillus
11.Water :
(a) Collection of water, Packing and dispatching of water sample.
12.Bacteriological Examination of :
(a) Examination of Pus, Abscess and wounds
(b) Milk (c) Air (d) water
13.Cultivation of Micro- Organism :
(a) Culture Medica (i) Composition (ii) Classification
14. Isolation and Inoculation according Techniques
Biochemical Test
 Test of Metabolism of Protein , amino acid , production of enzymes
 VP teat , MR test , Catalase test , Coagulates Test
 Gram stain, Negative Stain, Ziehl – Neelsen, Albert Stain.
Parasitology–(Theory)
“It is branch of medical science dealing with study of various human parasites.”
1. Morphology, Life Cycle, Symptoms Clinical Diagnosis & Laboratory Diagnosis
(a)Hook Worm (b)Round worm (c)Tape worm (d)Ent. Amobea-Histolytica(e)Entameoba
Coli(f)Plasmodia(g)Leishmania- donovani (h)Giardia- Lambila
Serology:(a)pregnancy Test(b)Widal Test(c)V.D.R.L Test (d)Elisa for HIV- I & II(e)RA & ASO
Test
 Paper 3
HAEMATOLOGY & BLOOD BANKING (2103)
HAEMATOLOGY:-
1. Introduction of Haematology
2. Collection of Blood
3. Red Cell Count (i) Method (ii) Calculation (ii) Haemocytometer
4. White cell count (T.L.C) (i) Method and Calculation
5. Differetal Leucocyte Count (D.L.C)(i)Normal Value and Morphology of White Cells (i) Counting
Method (iii) Staining Procedures
6. Packed Cell Volume (i) Normal Values & Macro & Micro Method
7. Estimation of Haemoglobin
Method-S.G, Chemical, Colonimetric&Gasometric etc. and Clinical Importance
8. Anticoagulation, MCV,MCH & MCHC & its Importance
9. Morphology of Normal abnormal Red cells
10. Method, Appearance & Normal Calues Reticulocyte Count
11. Coagulation Tests (i) Bleeding time, Prothombin Time WBC Coagulation time (ii) Clot Retration
Test, Platelet Count
12. Total Platelet Count (T.P.C) with Direct & Indirect Method
13. Urine Analysis (a)Physical, Chemical, Microscopic& Normal
14. Stool Examination (i) Microscopically Examination of Stool (ii) Chemical Examination Stool
(iii) Deference between Amoebic and bacillary Stool
15. Seman Analysis (i) Microscopical Examination of seman (ii)Normal & Abnormal Morphology
of Spermatozoa. (iii) Motility & Total Sperm Count (iv) Macroscopical Examination of Seman
(Amount of Seman, Colour, Rection, Viscocity)
16. Anaemia and Leukemia (a) Common Anatomical terms & Anatomical Position
Blood Banking
1. Blood Collection (i) Collection of Blood (ii) Storage of Blood (iii) Anticoagulation use for
collection of Blood (iv) Screening of doner
2. ABO & Rh Blood Group System – (i) ABO Grouping by Slide Method & Tube Method (ii)
Antigen and type of Antibodies (iii) Rh system with slide method (iv) Type of Antibodies (v)
One/Two stage Albumin Technique for Rh Factor
3. Cross Machine (i) Open slide Method (ii) Albumin tube Technique
4. Coomb,s Test (i0 Direct (ii) Indirect
5. Drawing of Blood for Donor
6. Blood Transfusion and its Reactions
7. Administration of Blood Bank
 Paper-04
Theory:- BASIC TECHNOLOGY & ETHICS (2104)
1. Microscope-Principal, Operation, care and use
2. Sterilization: General Principal of Sterilization, Classification, Physical, Mechanical Chemical
Method, Sterilization Media, Syringes, Glassware and Apparatus

Rote of laboratory in the health

Duties and responsibility of lab technician (a) General Duties (b) Specific Duties

3. First Aid and Safety Measures: (a)Aims and type and Diagnosis of First Aid (b) safety
Measures- Biological, Electrical ,Mechanical Chemical
4. Cod of Professional Conducts
5. Immunity: Types, Factor Effecting Immunity
6. Collection preservation and Storage of different body fluids
7. Communication: Public Relation, Patient relation and Physician, nursing staff relation, report and
record
8. Quality Control
9. Instrument (Internal): Hot air Oven, Auto-Clave
10. WHO and PHC

Ethics: Importance, Principle, Consideration

PAPER-05
Histo-technology(2105)
Theory 1. Introduction
2 Examination Method of Cell & Tissue
3. Tissue Processing (i) Collection of Specimen (ii) Fixation (iii) Labelling and
Clearing (iv) Dehydration
4. Fixation of Tissue (i) Simple Fixative and Cytological Fixative (ii) Micro
Anatomical Fixative
5. Staining (a) Staining of Tissues section (b) Theory of staining (c) Mounting of
section (d) Staining Technique
6. Section Cutting (i) Microtome and their Knives (ii) Mounting Section (iii)
Techniques of section cutting
7. Reception of Specimen, Preparation & Fixation and Restoration of colour
according Museum Technique
8. Autopsy Techniques (i) Processing of Tissues (ii) Preservation of Orange
 PAPER-06
BIO-CHEMISRY (2106)
1. Introduction of Biochemistry
2. Definition, Classification and Importance Metabolism in brief following
(a) Protein (b) Serum Album (c) Lipids
3. Analysis and Collection of Gastric Juice
4. Estimation of- (a) Total Protein (b) Serum Albumin (c) Globulin & A.G Ratio (d) Serum
Creatinine (e) Blood Sugar (God-Pog-Ortho-toludine & Folinwu Method )
5. Hormones –
(a) Definition of hormones (b) Function of Importance Hormones (c) T3, T4, T5 H
6. Enzymes and Co- Enzymes
7. Serum Amylase
8. Serum Electrolytes:- (a) Normal Blood Value Na+ (b) Normal Blood Value Cl – (c)
Normal Blood Value K + (d) Normal Blood Value Mg+ (e) Importance of Na+, Cl-, K+
9. Glocose Tolerance test (G.T.T.)
10. Liver Function test (i) Types & Classification (ii) S. GOT. S.G.P.T (iii) Bilirubin
Estimation (Direct & Indirect ) (iv) Estimation of Acid phosphatise & Alkaline
phosphatise (v) Jaundice Classification
11. D.N.A & R.N.A Their Importance
12. Urine Analysis (a) Normal & Abnormal & Constituents of Urine (b) Physical &
Chemical test of urine (c) Proteins in Urine (d) Occult blood in Urine (e) Urinary
Sediments
 DMLT - SECOND YEAR

Paper-01
BIOCHEMISTRY & CLINICAL PATHOLOGY.(2201)

Course Description Student should leave knowledge of carbohydrate

1. Protien and lipids vitamin. Minral and hormones as well as the relevant diagnostic tests.
Theory:-
Carbohydrates digestive and absorption metabolism of glucose glycolysis gluconeonesis . glycogen
Formation and breakdown stroge diseases maintinace of blood suger leave hormonal
influence,mellitus,inter conversion of mono saccharides(12hrs)
2. Digestion of protiens, urea synthesis, transminnation, metabolism of the following amion acid
Aromatic amino acid, sulphar containing amino-acid oxidation of fatty acid lipoproteins( 9hrs)
3. digestion and absorption of lipids. Synthesis of fatty acid acid oxidation of fatty acid liporprotiens.
4. Hormones Role of biological important hormones. Insulin glucose, epinephrine,thyroid growth
hormones steroid hormones.
5. Chemistry and biological role of Vitamins (7 hrs)
6. Minral metabolism iron,copper,calcium,mangnesium,phosphorus sodium,potassium,chloride,iodine
(9 hrs)
7. ETC and oxidative phosphorylation (3 hrs)
URINE
1. Composition of urine
Collection and preservation of urine
Changes in composition of urine relation to varises disease principal of dry chemistry
PRACTICAL
Complete urine analysis
a. Physical
b. Chemical Protein
Reducing substances
Ketone bodies
Blood pigments
Bile
c. Sediments
Use if dip sticks in urine analysis
2. Cavity fluids and miscellaneous specimens extra vascular fluids, normal composition
transudations and exudates
3. Cerebrospinal fluids and alteration in diseases
4. Semen analysis
5. Non- parasitological examination of stool including occult blood
6. Quality control-urine and extra vascular fluids
PRACTICALS:
1. Examination of CSF and reporting
2. Examination of cavity fluids and reporting
3. Semen analysis
4. Stool-Occult blood
5. Stool routine
6. Urine for Urobilinogen
7. Urine Bile salt, Bile pigment
 Paper (02)
2-HISTOPATHOLOGY & CYTOLOGY (2202)
COURSE DESCRIPTION A the end of the course the student will be able to fix process. Embed tissue
and make section for microscope student. He/She will also competent to make routine cytological
preparation.

THEORY
Introduction to histo pathological techniques
Reception of specimens
Fixation formalin fixatation
Tissue processing and embedding
Section cutting
Mounting and staining
Theory of H & E staining
PAS & PAP staining principal and uses
Stains for AFB [TB and leprosy]
Theory of frozen section prep ration

CYTOLOGY
THEORY
Principal of exfoliate cytology
Fixation of smears
PAP staining and identification of cells in a normal veginal smear
Preparations of smear of fine needle aspiration cytology
PRACTICALS
Embedding and preparation of blocks
Section cutting and use and care of microtone
H & E staining
PAS staining
AFB staining [TB and leprosy}
Frozen section and care of ctyosist
PAP staining MGG staining for fnac

Paper 03
3-MICROBIOLOGY, VIROLOGY MYCOLOGY & AVDVANCE SEROLOGY (2203)

OBJECTIVE
To give the student sound Knowledge of pathogenic miorobec, laboratory diagnosis, basic under standing
of virology mycology and advanced serologic techniques.
SYSTHMIC BACTERIOLOGY
Morpliology, isolation and identification of the pathogens cooer, bacilli, vibrio, spirochetes,
actionnomycetes Laboratory diagnosis.
Principles of antimicrobial therapy and biotic susceptibility tests. Common pathogenic fungi of skin
subcutaneous tissue Deep organ-laboratory diagnosis basic virology common viral diseases- transmission
– common and dispatch inoculation egg inoculation techniques.
Preservation of micro organisms
Organization of a microbiology laboratory
PRACTICALS
1. Maintenance of stock cultures
2. Identification of pathogenic organisms
 3. Methods of collection of clinical material for culture urine. Blood Sputum, C.S.F. throat swab,
faeces, and body fluids.
4. Separation of sera, preservation and transport for serological tests.
5. Antibiotic susceptibility tests
6. Basic techniques of collection oaspecimens for direct examination of pathogenic fungi KOH.
Lactopheoal blue method.
7. Cultivation of fungi
8. Basic technique of collection and transport of specimens for virology studies.
9. Diagnosis of viral infections isolation and serological tests.
10. Advanced serological technique cliza, immunoelectropiaresis.

Paper :-04
4 -COAGULATION & TRANSFUSSION MEDICINE (2204)
COURSE DESCRIPTION At the end of the course the student will be familiar with investigation of
coagulation disorder and will also understand the principles of immunohematology He/She will be
competent to handle routine blood bank.
Organization and procedures
COAGULATION DISORDER
Principles of blood coagulation and haempostasis
Disorder of coagulation and hemostasis.
Laboratory diagnosis of bleeding disorders.
Quality control in coagulation laboratory.
PRACTICALS
Whole blood coagulation time
Clot retraction and clotlysis
Bleeding time
Tourniquet tests
One stage prothrombin time
Partial thromboplastin time with correction
Factor assay
Platelet disorders
Disorders of platelets and laboratory diagnosis
PRACTICALS
Investigation of platelets disorders including sample methods to assess platelets adhesion,
aggregation and factor release.
1. BIOCHEMISTRY & CLINICAL PATHOLOGY
Course Description Student should leave Knowledge of carbohydrate. Protein and lipids
vitamins. Mineral and hormones as well as the relevant diagnostic tests.
THEORY
1. Carbohydrate digestion and absorption metabolism of glucose glucolysis gluconeognesis.
Glycogen formation and breakdown glucogen storage diseases, maintenance of blood
sugar leaves hormonal influence, mellitus, inter conversion of mono saccharides (12hrs)
2. Digestion of proteins. Urea synthesis, transmination, metabolism of the following amino
acid Aromatic amino acid, sulphur containing amino-acid in born error of metabolism
(10hrs)
3. Digestion and absorption of lipids. Synthesis of fatty acid oxidation of fatty acid
lipoproteins(9hrs)
 4. Hormones- Role of biological Important hormones. Insulin glucose, epinephrine, thyroid
growth hormones steroid hormones.
5. Chemistry and biological role of vitamins (7hrs)
6. Mineral metabolism iron, copper, calcium, magnesium, phosphorus sodium, potassium,
chloride, iodine (9hrs)
7. ETC and oxidative phophorylation (3hrs)
PRACTICALS
1. Estimation of blood urea ceratinine uric acid calcium, phosphorus and chloride. (9hrs)
2. Sodium and potassium estimation by flame photometer (8hrs)
3. Blood glucose estimation by flame photometer (8hrs)
4. Theory of serum electrophoresis (6hrs)
5. Demonstration of paper chromatography (8hrs)

Paper 05
5-IMMUNOLOGY HAEMATOLOGY & TRANSFUSSION MEDICINE
(2205)
THEORY
Principles of blood groups and antigen antibody reaction
Genetics in blood banking
ABOH blood group system
Rhesus blood group system
Other red cells antigens and antibodies
Transfusion of antibodies
Coonsbs tests
Identification of antibodies
Transfusion reactions and investigation of transfusion reaction
Haemolytic disease of new born
Blood donor selection and screening of blood donor
Diseases transmined by blood transfusion and their laboratory diagnosis
Blood components and use
Blood bank organization donor motivation and auditing blood bank
PRACTICALS
Blood collection and preservation using different anticoagulants and
Preservation solution.
Components prepratiuon
ABO grouping
Rh typing0
Antibody detection and titration
Coombs tests
Compatibility testing cross matches
Investigation if transfusion reactions
Investigation of hemolytic disease of new born
HbsAG and HUV antibody testing in blood bank