Fluorescence Micros
Fluorescence Micros
Microscopy
Emission filter
• It only passes only those wavelengths are emitted from a fluorophore.
• It blocks all unwanted wavelengths
Dichroic mirror
• It is a type of accurate color filter.
• It only passes small range of colors and reflects other colors.
Cell biology Lokender Kumar Ph.D.
Light path…
1. A high-intensity Mercury lamp
2. The exciter filter transmits only blue
lights to the specimen and blocks out all
other colors.
3. The blue light is reflected downward to
the specimen by a dichroic mirror,
which reflects the lights of certain colors
but transmits light of other colors.
4. The specimen is stained with a
fluorescent dye