05 - Enzymes
05 - Enzymes
Chapter 05
Enzymes
The stuff you need to know in this chapter:
Core:
• Define the term catalyst as a substance that increases the rate of a chemical reaction and is not changed by the
reaction
• Define enzymes as proteins that function as biological catalysts
• Describe why enzymes are important in all living organisms in terms of reaction speed necessary to sustain life
• Describe enzyme action with reference to the complementary shape of an enzyme and its substrate and the formation
of a product (knowledge of the term active site is not required)
• Investigate and describe the effect of changes in temperature and pH on enzyme activity
• Investigate and describe the use of biological washing powders that contain enzymes
Extended:
• Explain enzyme action with reference to the active site, enzyme-substrate complex, substrate and product
• Explain the specificity of enzymes in terms of the complementary shape and fit of the active site with the substrate
• Explain the effect of changes in temperature on enzyme activity in terms of kinetic energy, shape and fit, frequency of
effective collisions and denaturation
• Explain the effect of changes in pH on enzyme activity in terms of shape and fit and denaturation
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2. Define “catalyst”
3. Many reactions in living organisms could happen even if enzymes weren’t there. Despite this, organisms
couldn’t live without enzymes. Explain why they are essential to life.
4. Draw a diagram to explain the “lock and key” mechanism by which enzymes function.
In your diagram label the following:
enzyme, substrate, enzyme-substrate complex, product, active site
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6. State why enzymes are useful in soap powders for washing clothes.
7. State the name of the enzymes present in biological washing powders that break down the following food
materials:
Protein
Starch
Lipids
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2. State how an increase in temperature affects the number of collisions of a substrate with the active site of
an enzyme
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Rate of reaction
c
a
b
Temperature
Explanation of trend
4. The peak of the graph shows the temperature at which the rate of reaction is fastest. What phrase
describes this temperature for this particular enzyme?
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6. Students sometimes say that enzymes are “killed” by high temperatures or a certain pH. What word is
better? Explain why.
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