PhysioEx Exercise 4 Activity 2 26/6/23 09:52

PhysioEx Lab Report

Exercise 4: Endocrine System Physiology
Activity 2: Plasma Glucose, Insulin, and Diabetes Mellitus
Name: Pablo Palacios
Date: 26 June 2023
Session ID: session-911c8dc6-df5e-0e42-03a0-9fca3f9c4a41

Pre-lab Quiz Results

You scored 100% by answering 5 out of 5 questions correctly.

1 Which of the following statements is false?

You correctly answered: Insulin is a hormone secreted into the stomach to aid with
starch digestion.

2 Which of the following statements is true?

You correctly answered: All of these statements are true.

3 A diagnosis of type 1 diabetes mellitus implies that

You correctly answered: the pancreas is not producing sufficient insulin.

4 A diagnosis of type 2 diabetes mellitus implies that

You correctly answered: the cells of the body are unresponsive to circulating insulin.

5 Glucagon is a hormone

You correctly answered: that opposes the action of insulin.

Experiment Results
Predict Question
1 Predict Question: To measure the amount of plasma glucose in a patient sample

Your answer: the optical density of the sample will be measured and the glucose
concentration will be extrapolated from the glucose standard curve.

Stop & Think Question

1 Why does the color in the tubes vary?

https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex4/act2/ Página 1 de 4
PhysioEx Exercise 4 Activity 2 26/6/23 09:52

You correctly answered: The tubes contain different amounts of glucose.

Experiment Data
Tube Optical Density Glucose
(mg/deciliter)
Part 1 1 0.30 30
2 0.50 60
3 0.60 90
4 0.80 120
5 1.00 150
Part 2 1 0.73 105
2 0.79 115
3 0.89 132
4 0.83 122
5 0.96 143

Post-lab Quiz Results

You scored 100% by answering 6 out of 6 questions correctly.

1 A male patient has had successive fasting plasma glucose readings of 115, 110, and
122 mg/dl. The healthcare provider will inform him that

You correctly answered: he appears to have impairment or borderline impairment of

insulin-mediated glucose uptake by his cells.

2 To obtain an accurate spectrophotometric measurement of the glucose concentration

in the sample

You correctly answered: heparin is added to prevent blood clots.

3 In the spectrophotometric assay used in this experiment, the __________ as the glucose
concentration in the sample increases.

You correctly answered: optical density increases.

4 A female patient has had successive fasting plasma glucose readings of 130, 140, and
128 mg/dl. The healthcare provider will inform her that

You correctly answered: she has developed diabetes.

https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex4/act2/ Página 2 de 4
PhysioEx Exercise 4 Activity 2 26/6/23 09:52

5 To maintain plasma glucose homeostasis

You correctly answered: insulin-mediated transport of glucose into cells acts as

negative feedback when plasma glucose levels rise.

6 A laboratory technician withdraws a blood sample from a vein in your upper arm
knowing that

You correctly answered: the plasma glucose concentration will be the same in both the
arm vein and the index finger.

Review Sheet Results

1 What is a glucose standard curve, and why did you need to obtain one for this
experiment? Did you correctly predict how you would measure the amount of plasma
glucose in a patient sample using the glucose standard curve?

Your answer:

La curva estándar de glucosa, es una herramienta utilizada para medir los niveles de
glucosa plasmática en ayunas, tomados de varios pacientes para diagnosticar la
presencia o ausencia de diabetes.

2 Which patient(s) had glucose reading(s) in the diabetic range? Can you say with
certainty whether each of these patients has type 1 or type 2 diabetes? Why or why
not?

Your answer:

Tanto los pacientes 3 como 5 tienen niveles de glucosa en un rango de diabetes, no

obstante, no existe la información suficiente como para distinguir qué tipo.

3 Describe the diagnosis for patient 3, who was also pregnant at the time of this assay.

Your answer:

Puede que presente diabetes gestacional.

4 Which patient(s) had normal glucose reading(s)?

Your answer:

El primer paciente.

5 What are some lifestyle choices these patients with normal plasma glucose readings

https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex4/act2/ Página 3 de 4
PhysioEx Exercise 4 Activity 2 26/6/23 09:52

might recommend to the borderline impaired patients?

Your answer:

Una dieta balanceada, en conjunto con ejercicio frecuente.

https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex4/act2/ Página 4 de 4