Gilson FC 203B

Fraction Collector
User’s Guide

LT1731/©2003 Gilson, Inc. All rights reserved June 2003

Table of Contents

Declaration of Conformity

1 Introduction
Unpacking ............................................................................. 1-2
Customer Service ................................................................. 1-3
Technical Specifications ..................................................... 1-4

2 Installation
Plumbing Setup .................................................................... 2-1
Hardware ....................................................................... 2-2
3-Way Valve Installation ............................................. 2-4
Tubing ............................................................................. 2-7
Electrical Setup ..................................................................... 2-9
Rear Panel Description ................................................ 2-9
Input/Output (I/O) Port ............................................. 2-10
Wiring to Other Equipment ........................................ 2-13
Power Connection ........................................................ 2-21

3 Operation
Front Panel ............................................................................ 3-2
Command Hard Keys .................................................. 3-2
Numeric Hard Keys ...................................................... 3-2
Soft Keys ......................................................................... 3-2
Help Key ......................................................................... 3-3
Start Up ................................................................................. 3-4
Collection Options ............................................................... 3-5
Entering Numbers ......................................................... 3-5
Review or Change Options ......................................... 3-5
Review or Change Racks ............................................. 3-6
Collection Into Same or
New Tubes for Each Cycle .......................................... 3-6
Choosing Collection Modes ......................................... 3-7
Building Collection Windows ..................................... 3-7
Ready to Start ................................................................ 3-7
During the Run .................................................................... 3-9
Manual Override ........................................................... 3-9
Power Failure ................................................................. 3-9
 Cycle Collection ................................................................... 3-10
Collect into Same Set Tubes ........................................ 3-11
Collect into New Set Tubes ......................................... 3-11
Ready-to-Run Display .................................................. 3-12
Collection Modes ................................................................. 3-13
Choosing Time Collection Mode ................................ 3-14
Choosing Drop Collection Mode ................................ 3-16
Choosing Peak + Time Collection Mode ................... 3-18
Choosing Peak + Drop Collection Mode .................. 3-24
Choosing Manual Collection Mode ........................... 3-30
Collection Windows ............................................................ 3-33
Building No Windows .................................................. 3-36
Building One Window ................................................. 3-36
Building More Than One Window ............................ 3-37
Deleting Windows ........................................................ 3-39
Nonsense Windows ...................................................... 3-39

4 Maintenance
Cold-Room Use .................................................................... 4-2
Chemical Compatibility ...................................................... 4-3
Transporting the Fraction Collector ................................. 4-4
Replacing a Fuse .................................................................. 4-5

5 Troubleshooting
Troubleshooting ................................................................... 5-2
Repair and Return Policies ................................................ 5-7

Appendix A Racks
Thermostated Racks ............................................................ A-9

Appendix B Parameter Review Menu

Appendix C Technical Menu

Appendix D Service Menu

Appendix E Dispense Head End-Fitting Installation

Replacement ......................................................................... E-2
Repositioning ........................................................................ E-3
Appendix F GSIOC Interface
GSIOC Commands .............................................................. F-2
Immediate Commands ................................................. F-2
Buffered Commands ..................................................... F-5

Appendix G Multiple Column Option

Set-up ..................................................................................... G-2
Column Option Display ............................................... G-2
Install Column Adapter ............................................... G-3
Connect Outlet Tubing ................................................ G-4
Install Rack ..................................................................... G-5
Align Outlet Tubings .................................................... G-5
Begin the Run ....................................................................... G-6
End the Run .......................................................................... G-6
Return to Single Column Collection ................................ G-6

Appendix H Peak Detection Using Slope or Millivolt Level

Detection Using Slope (Peak Detection Algorithm) ...... H-2
Master Slope ................................................................... H-3
Peak Phases .................................................................... H-4
Maximum Peak Width ................................................. H-5
Special Cases .................................................................. H-5
Detection Using Millivolt Level ......................................... H-6

Appendix I Autopreparative Collection Controlled

by 712 HPLC Control Software
Connecting System Components ...................................... I-2
GSIOC Connections ...................................................... I-2
Contact Connections .................................................... I-2
Detector Connections ................................................... I-3
Programming the 203B ....................................................... I-5
Example 1
Collection of Each Cycle Into Same Set of Tubes .... I-5
Example 2
Collection of Each Cycle Into a
Different Set of Tubes ................................................... I-8
Coordinating with 712 HPLC Control Software ........... I-11
Configuration ................................................................. I-11
Number of Loops ........................................................... I-11
Mobile Phase .................................................................. I-12
Contact Events ............................................................... I-13
GSIOC Events ................................................................ I-13
Method Listing .................................................................... I-16
Appendix J Autopreparative Collection Controlled by 305 Pump
Connecting System Components ...................................... J-2
GSIOC Connections ...................................................... J-2
Contact Connections .................................................... J-2
Detector Connections ................................................... J-3
Programming the 203B ....................................................... J-5
Example 1
Collection of Each Cycle Into Same Set of Tubes .... J-5
Example 2
Collection of Each Cycle Into a
Different Set of Tubes ................................................... J-8
Coordinating with 305 Pump ........................................... J-11
Set Up Pump Hardware .............................................. J-11
Set Up Contact .............................................................. J-12
Set Up Program File ...................................................... J-12
Program Sheet ............................................................... J-12

Appendix K Collecting Sharp Narrow Peaks

Appendix L Replacement Parts and Accessories

Declaration of Conformity

Application of Council Directives:

89/336/EEC, 73/23/EEC

Standards to which Conformity is Declared:

EN61326, EN61000-3-3, EN61000-3-2, EN61010-1

Manufacturer’s Name ........................................... Gilson, Inc.

Manufacturer’s Address ....................................... 3000 W. Beltline Highway

Middleton, WI 53562

EC Office Address .................................................. Gilson S.A.S.

19 Avenue des Entrepreneurs, B.P. 145
F-95400 Villiers-le-Bel, France

Type of Equipment ................................................. Laboratory Equipment

Model. ....................................................................... FC 203B Fraction Collector

Beginning with Serial Number: 179A6001

Month and Year of Manufacture: January 1996

I, the undersigned, hereby declare that the equipment

specified above conforms to the above Directives and Standards.

Place: Middleton, WI (USA) Michael Jacquart

Issue Date: January 1996 Senior Vice President
Corporate Technology Development
Introduction 1

Welcome to improved fraction collecting. The Gilson FC 203B Fraction Collector is simple
to use and straightforward to learn. Its sophisticated design makes it flexible enough to
adapt to your collection goals. The fraction collector is designed to make every operation
clear and efficient.

You can get information about the fraction collector from these sources:

• The HELP key on the 203B’s keypad offers specific, on-line instructions during setup
and operation.

• The 203B Quick Reference Guide answers key points quickly. Two copies are included.

• This User’s Guide describes how to operate the 203B.

• Gilson’s customer service team is available to answer your questions. Refer to page 1-3
for contact information.

Note: If you’re controlling the FC 203B Fraction Collector via Gilson UniPoint™ System
Software, you can obtain the following technical notes from your Gilson representative:
Fraction Collector Control (part number LT21373) and Large-Volume Preparative System
Control Using a 30x Pump for Repetitive Injection (part number LT21375).

1-1
 Introduction 1
Unpacking

Unpacking
Unpack the fraction collector and accessories
carefully from the carton. Cross-check the contents
against the standard equipment checklist shown
below and against your purchase order’s optional
accessory list to verify that all parts are included
and undamaged.

Do this now, even if your fraction collector will not

be used immediately. Many carriers must receive
concealed damage claims within seven days of
delivery.

The fraction collector is shipped with its drain

trough and dispense head already installed.

Your standard accessories include the following:

• rack holder assembly (with antlers)

• 80-cavity collection plate
• two Code 1 racks (for 80, 13 x 100 mm tubes)
• tube retaining bar (for Code 1 rack)
• inlet tubing, 0.031" (0.8 mm) ID, with two
Omnifit™-type end fittings
• polypropylene pan
• terminal block connector
• spare fuse
• power cord
• FC 203B Fraction Collector User’s Guide
• FC 203B Fraction Collector Quick Reference Guide
(2 copies)
• tube gauge

If you ordered a diverter valve with the fraction

collector, the standard accessory package also
includes a 3-way valve kit.

Please retain all packing material so unit may be

shipped safely, if necessary.

1-2
Introduction 1

Customer Service
Customer Service
Gilson, Inc. and its worldwide network of
authorized representatives provide customers with
four basic types of assistance: sales, technical,
applications, and instrument repair.

If you need assistance, please contact your Gilson

representative or if you are in the United States
call the Gilson Customer Service Department at
800-445-7661 or 608-836-1551. You can also
contact the Gilson Customer Service Department via
its e-mail address: [email protected]. Specific
contact information can be found on the Gilson
web site at www.gilson.com. To help us serve you
quickly and efficiently, please refer to the Before
calling us section on page 5-7.

1-3
 Introduction 1
Technical Specifications

Technical Specifications

Warning: Changes or modifications to the fraction

collector not expressly approved by Gilson could void
your factory-authorized warranty.

The fraction collector has been tested and found

to comply with the limits for a Class A digital
device, pursuant to Part 15 of the FCC commercial
environment. The fraction collector generates,
uses, and can radiate radio frequency energy and,
if not installed and used in accordance with the
instructions, may cause harmful interference to
radio communications. Operation of the fraction
collector in a residential area is likely to cause
harmful interference; in which case, the user will
be required to correct the interference at the user’s
own expense.

Shielded cables must be used with the fraction

collector to ensure compliance with the Class A
FCC limits.

1-4
 Technical Specifications

1-5
1
Introduction
 Technical Specifications

1-6
Introduction
1
Installation 2

Plumbing Setup
Throughout the plumbing setup, refer to the diagram below.

2-1
 Installation 2
Plumbing Setup

Hardware

Pan

Set the pan in place so it rests on the floor of the

fraction collector. Note that the pan will fit properly
only when positioned with its beveled edge facing
the front of the instrument.

Rack holder

Secure the rack holder with two thumbscrews

(provided) in the upper position for the standard
Code 1 rack (for 13 mm tubes) and for Code 0, 2, 4,
11, and 14 racks.

If you are using a Code 30, 32 (with 13 x 65 mm

tubes), or 33 rack, you must install the special rack
holder designed for thermostated racks.

Do not install the rack holder when using Code 15,

20, 21, 23, 23W, 24, 28, 29, 29 LE, or 29 SE racks.

Rack

Set the rack in place.

Most tubes with a maximum 13 mm outer diameter

(OD) will fit the Code 1 rack. Gilson recommends
Kimble #73500 or Corning #99445 test tubes.

Use the tube gauge located in the accessory package

to check the diameter of your tubes. If a tube does
not slide through the tube gauge, it’s too big to fit a
Code 1 rack.

You’ll need a different rack if other size tubes will

be used. Look in Appendix A for information about
other racks.

Note: Code 0, 2, 4, 11, and 14 racks fit on the

antlers of the rack holder. To install one of the
stainless steel racks that fit on the antlers, see page
A-3.

2-2
Installation 2

Plumbing Setup
The Code 15 microplate holder attaches with
thumbscrews to the back face of the fraction collector.
If you are using a standard microplate, attach the
Code 15 plate holder in the upper position. If you are
using a deep-well microplate, attach the Code 15
plate holder in the lower position.

Code 1, 20, 21, 23, 23W, 24, 28, 29, 29 LE, and 29
SE racks fit directly in the pan.

Code 30, 32 (with 13 x 65 mm tubes), and 33 racks

fit on the special rack holder for thermostated racks.

Code 32 rack (when used with 13 x 100 mm tubes)

fits directly in the pan.

Tube retaining bar

When using the Code 1 rack (for 13 x 100 mm tubes),

set the tube retaining bar in place, as shown on page
2-1. This bar is required to keep the tubes in an
even rectangular pattern.

2-3
 Installation 2
Plumbing Setup

3-Way Valve Installation

If you purchased a 3-way valve with your fraction
collector, you can install it now. A 3-way valve is
highly recommended for collection when using
collection windows. It is required if you are
collecting into a microplate installed on a Code 15
plate holder.

The 3-way valve prevents spillage when effluent is

diverted to waste and simplifies collection of pure
peaks.

Note: The 3-way valve operates at flow rates up to

20 mL/min. and pressures up to 20 psi. The dead
volume of the 3-way valve is 3.5 µL from the
common (COMM) port to the normally closed (NC)
port. Refer to the diagram on the left.

Before installing the 3-way valve, turn off power to

the fraction collector using the rear panel power
switch.

Gently pull the dispense head away from the drain

trough.
Omnifit™-type Fitting
Inlet tubing

From the valve’s accessories, locate the inlet tubing

that has fittings on both ends.

Using the illustration at left for reference, attach

one end of the inlet tubing to the valve source inlet.
To do this, you’ll have to remove the plastic nipple
from the 3-way valve.

Fingertighten the fitting. Pull gently on the tubing

to make sure the fitting is firmly seated.

Connect the other end of the inlet tubing to the

outlet of your detector. It is good practice to keep
the length of tubing from the detector outlet to the
dispense head as short as possible. But remember to
leave enough tubing to account for movement of
the dispense head.

2-4
Installation 2

Plumbing Setup
Outlet connection

The outlet tubing is already connected to the 3-way

valve. Place the other end of the outlet tubing into a
drain or waste receptacle.

Assembly

You are now ready to install the assembly on to the

top of the dispense head. Use the drawing below
for reference.

Remove any connections to the top of the dispense

head. Note the small mounting tab on the drop
detector cover next to the threaded female fitting.
When properly installed, this tab protrudes through
the hole on the L-bracket of the 3-way valve.

Install the 3-way valve by fingertightening the

stainless steel fitting into the dispense head. You
may encounter some resistance after a few turns. If
so, use a 5/16" open-end wrench to completely
tighten the valve. Do not over-tighten; tighten only
until the assembly is stable.

2-5
 Installation 2
Plumbing Setup

Electrical connection

Now make the electrical connection for the 3-way

valve. Locate the socket in the fraction collector
into which you will insert the plug from the 3-way
valve. To do this, follow the dispense head’s spiral
cord to the inside of the fraction collector. Just to
the right of the cord, you’ll see a socket containing
three pins.

Note that the plug from the valve has two small
tabs on one side. Insert the plug into the socket
with the two tabs facing toward the left.

The fraction collector is now ready for operation

with a 3-way valve. Once the electrical connection
is made, the fraction collector knows the 3-way
valve is in place and acts accordingly. The dispense
head will not travel to the drain trough during
drain periods but is always located over the current
tube. Effluent is diverted to drain through the 3-way
valve’s outlet tubing.

The fraction collector automatically detects the

presence of the 3-way valve. The instructions you
give to the fraction collector are the same, whether
or not the 3-way valve is installed.

Note: In the event of power failure, effluent is

2-6 diverted to drain and is not collected.
Installation 2

Plumbing Setup
Tubing

Inlet tubing

Note: If you installed a 3-way valve, you’ve already

connected the inlet tubing to the fraction collector
and the detector. Proceed with Drain trough
connection on the next page.

Using the diagram, locate the inlet tubing. It has

Omnifit™-type fittings on both ends.

Omnifit™-type Fitting

One end of the inlet tubing extends 11/32" beyond

the fitting. Screw this end into the threaded opening
at the top of the dispense head. Fingertighten only.

Note: The length of tubing that extends from the

fitting is critical to accurate drop collection. If the
tip becomes damaged, use the tubing gauge
described in Appendix E to position the fitting on
the replacement tubing.

The ID of the inlet tubing supplied with the fraction

collector is 0.031" (0.8 mm).

2-7
 Installation 2
Plumbing Setup

Detector connection

If a detector is connected to your fraction collector,

it is good practice to keep the length of tubing from
the detector outlet to the dispense head as short as
possible.

Note: Remember to leave enough tubing to account

for movement of the dispense head.

The outlet tubing from Gilson detectors can be

directly coupled to the fraction collector’s inlet
tubing, since both have Omnifit™-type fittings at
their ends. Use the 1/4-28 coupler that came in
your detector’s accessory package. If necessary, a
package of five couplers can be ordered from
Gilson (part number F1410050).

Drain trough connection

Cut the drain tubing supplied with the fraction

collector into two appropriately sized pieces.
Connect one end of a piece of tubing to the drain
port of the drain trough and place the other end
into a drain or container.

2-8
Installation 2

Electrical Setup
Electrical Setup
Rear Panel Description
Connections on the FC 203B Fraction Collector’s rear
panel include:

1 Gilson Serial Input/Output Channel (GSIOC) port

2 Input/Output (I/O) port
3 Fuse drawer
4 Power switch
5 Power receptacle

Note: Please read this entire section and refer to the

rear panel diagram below before making any
electrical connections. Do not attach the fraction
collector’s power cord until all other connections
have been made.

Rear Panel Diagram

2-9
 Installation 2
Electrical Setup

Input/Output (I/O) Port

You can take advantage of the powerful built-in
features of your fraction collector when you connect
other equipment to the multipurpose I/O port.

The port has 10 contacts, numbered 1–10. All of the

connections are paired contact closures (no voltage)
except for the DET (detector) input which requires
an analog detector signal (±10 mV to ±100 mV).

TTL signals can be used as long as the voltage is

no greater than 5V.

Some uses for the I/O contact pairs are listed

below.

• When you attach a detector to the DET input

(1-2 pair), the 203B can collect peak-containing
fractions. Data from the detector can be digitized
and sent to a controller for quantification of
peaks. This input was zeroed at the Gilson
factory.

• When you connect a remote switch contact to

Input A (3-4 pair), other equipment can signal
the 203B to begin collection and to advance the
dispense head.

• When you connect a remote switch contact to

Input B (5-6 pair), other equipment can signal
the 203B to end collection.

• When you connect your chart recorder’s event

mark input to Output 1 (7-8 pair), you can
indicate tube advance on the chart.

• When you connect another device to

programmable Output 2 (9-10 pair), the 203B
can automatically start and stop that device.

In the section called Wiring to Other Equipment,

you will find information about how to make these
I/O connections work for you.

2-10
Installation 2

Electrical Setup
The diagram below shows the location of the
multipurpose I/O contacts. Make the I/O
connections to the terminal block connector that
attaches directly to the fraction collector. You will
find detailed installation instructions in the next
section.

Items you’ll need

To make connections to the multipurpose I/O port,

you’ll need:

• terminal block connector (part number

638310512) included with your fraction collector
accessories

• 2-conductor interconnect cable for each

connection you’ll make. Use any 2-conductor
cable with a gauge from 22–30G for each wire.
You can purchase a 6-foot piece of suitable
cable (part number 709910206) or a package of
five cables with identification markers (part
number 36078155) from Gilson.

• wire insulation stripper

• small-blade screwdriver

• additional items for connection to other devices;

they’ll be specified in the text

2-11
 Installation 2
Electrical Setup

Making connections

To prepare wire for each connection, cut it into

pieces of appropriate length; then strip about 1 cm
of insulation from each end.

Insert each wire into the appropriate slot on the

terminal block connector. Push each wire all the
way in; then tighten the corresponding pin screw.

Note: Be sure to maintain the correct orientation

of the terminal block connector relative to the
multipurpose I/O port. With the pin screws facing
you and the wires entering from the bottom, the
DET input is at the far left.

Note: Attach all wires before joining the terminal

block connector to the multipurpose I/O port on
the fraction collector’s rear panel.

Connect the terminal block connector with the

wires facing down and the pin screws facing away
from the 203B. This prevents spillage from dripping
onto the contacts.

Push the terminal block connector as far as it will

go. The block is designed to fit snugly into its
receptacle.

A configuration using all five inputs and outputs

would look like the diagram below.

2-12
Installation 2

Electrical Setup
Wiring to Other Equipment

Detector input

To separate peaks as they elute from your column, a

detector is required. When the DET input (1-2 pair)
of your fraction collector is linked to a detector, you
can specify whether you want to collect peak
fractions or peak and non-peak fractions.

Detectors generally have one of two types of output

connectors. Using the accompanying figures,
determine which output connectors are used on
your detector.

One type of detector has screwable binding posts

built into the rear panel of the detector. To attach
the fraction collector to these outputs, connect lead
1 to the “+” post on the detector and lead 2 to the
GND post. Tighten the posts securely. If you’re not
using a Gilson detector, refer to the Note and diagram
on the next page.

The other type of connector, like the one on most

Gilson detectors, has fixed binding receptacles. To
attach this type, you’ll need a Pomona cable
adapter (part number 6374022611).

Pomona Cable Adapter

2-13
 Installation 2
Electrical Setup

Make the connections to your fraction collector as

shown below. Be sure to connect lead 1 to “+” and
lead 2 to GND.

Note: If you’re using a non-Gilson detector, you

may need to make another connection to reduce
ground noise. Connect a wire between the DET
input 2 (GND) and the metal screw in the lower
left or right corner of the fraction collector. This
ensures that the detector is properly grounded. If
the detector is not grounded, collection problems
may occur as a result of the different electrical
references for the fraction collector and detector.

Now you’re ready to collect fractions in one of

the peak detection modes. You’re also set up to
convert the peak information into digital data for
quantification by the Gilson HPLC system control
software. See Appendix I for more information.

2-14
Installation 2

Electrical Setup
Remote start/advance and remote end inputs

As an alternative to front panel control of starting,

advancing, and ending, you can control these
functions remotely:

• Input A (3-4 pair) controls remote start and

advance.
• Input B (5-6 pair) controls remote end.

When making the Input A and Input B connections,

the source must provide either contact closure (no
voltage) signals between “+” and GND or a
correctly polarized TTL (0, 5V) compatible signal (0
activates input).

Full-function remote control can be achieved

through the GSIOC port. See Appendix F for details.

Event mark output at tube advance

You can mark tube advance directly on your data

chart using the FC 203B Fraction Collector event
mark output (Output 1, 7-8 pair). The connections
you make for event marks depend on the data unit
in the system. Refer to the appropriate section
below.

Gilson 506C System Interface and 712 HPLC

System Control Software. In systems with a Gilson
506C System Interface, use a 2-conductor
cable to make the event mark connection.
Connect the fraction collector event
mark output to Input A of the 506C. In
the Contact Events box of the Gilson 712
HPLC System Control Software, label
Input A as the “FC 203B Event Mark,”
but don’t enter any timed events for this
input. In the Contact dialog of a
UniPoint control method, label the
contact and choose the Event check box. Event mark connection
between fraction collector and 506C

2-15
 Installation 2
Electrical Setup

Gilson Detectors. In systems with a detector as the

data unit, use the event/autozero cable (part
number 631004021) supplied with the detector to
make the event mark connection. The black and
red leads on this cable attach to the fraction
collector event mark output (7-8 pair). Attach
either wire to either slot.

Note: If you are collecting in a peak mode, do not

attach the event mark output pair to the detector.
Instead, connect the event mark output pair directly
to your chart recorder or integrator. This will
prevent the event mark being interpreted as a peak.

Event Mark Connection between

fraction collector and detector

Non-Gilson data unit (e.g., chart recorder,

integrator, non-Gilson software) or Gilson 305
Pump. In these systems, use 2-conductor cable to
connect the 203B event mark output to the
appropriate input pair on the data unit. If your
data unit cannot accept input from the fraction
collector, you may connect the fraction collector
event mark output pair to the appropriate event
input pins of your detector. When set up this way,
the data unit may interpret event marks as negative
peaks that will appear on the actual detector trace.

Note: The 305 does not accept the input from the
fraction collector event mark output. Therefore,
connect the fraction collector event mark output to
a chart recorder, integrator, or detector.

Note: The event input pins on the Kipp & Zonen

BD 11E/12E recorders available from Gilson, as well
as most other Kipp & Zonen recorders, can accept
2-16 input from the fraction collector event mark output.
Installation 2

Electrical Setup
Event mark output at peak start

If you are collecting in peak mode, you can get an

event mark at the start of a peak, in addition to the
event mark that indicates tube advance at the end
of a peak.

• Make the event mark at tube advance connection

between the 203B and your data unit as described
above.

• Short Output 2 (9-10 pair) to Output 1 (7-8 pair)

of the 203B.

• Using the Technical menu (EDIT 2), select the

Out 2 ON at peak start option for programmable
Output 2. See the next section for details on
programmable Output 2.

Programmable output

The fraction collector has several jobs to accomplish

during normal operation. It may be waiting, draining,
or collecting.

Programmable Output 2 (9-10 pair) can direct other

equipment (a chart recorder or peristaltic pump, for
example) to be active only when the fraction collector
is doing one of those jobs. By selecting one of the
Output 2 options, you can instruct a device to be:

1 ON after an initial wait period (Wait 1)

2 ON during an initial wait period (Wait 1)
3 ON during a collection window
4 ON during an entire run (wait, drain and
collect)
5 ON only when the 203B is not in a run
6 ON (pulsed) at the end of a run
7 ON (pulsed) when a peak is detected

2-17
 Installation 2
Electrical Setup

The following diagram illustrates each of the

options (1–7). Wait 1 identifies the time before the
start of the first collection window.

To use the programmable output, connect the leads

from Output 2 (9-10 pair) to a contact-activated
input of another device. Connect either lead to
either input pin on the peripheral device.

2-18
Installation 2

Electrical Setup
Then specify which one of the seven options to use.
Access the Technical menu by pressing EDIT then
2. You’ll see:

GSIOC unit ID =
Next Quit

Scroll through the menu screens by pressing Next,

until you see:

Out 2 on [1 of seven choices]

Next No Quit

To select the displayed choice, press END or Next.

If you want one of the other choices, repeatedly
press No until the screen displays the choice you
want. Then press END or Next to select that choice.
To exit the Technical menu, press Quit.

Examples:

• To turn on the strip chart after the initial wait

period, you would connect Output 2 to an
input pin of the strip chart and select the Out 2
ON after Wait 1 option.

• To turn on a pump during a run, you would

connect Output 2 to an input pin on the pump
and select the Out 2 ON during run option.

• To automatically start a new run after the

previous one completed, you could connect
Output 2 to Input A of the fraction collector
and select the Out 2 ON after end of run
option.

2-19
 Installation 2
Electrical Setup

GSIOC interface

The Gilson Serial Input/Output Channel (GSIOC)

is a bidirectional communications link which
connects a master module (e.g., computer) with as
many as 32 slave devices (in this case, a fraction
collector).

The master communicates with one slave at a time.

Each slave is identified by a unique unit ID code,
designated by a number between 0 and 63. You
can find or reset the GSIOC ID number in the
Technical menu (EDIT 2); see Appendix C.

Using GSIOC, you can monitor and control the

activity of the fraction collector and other Gilson
equipment from a Gilson controller.

Appendix F describes in detail the commands used

to control your fraction collector from a master
module.

2-20
Installation 2

Electrical Setup
Power Connection
After you’ve made all the necessary connections,
you’re ready to attach the power cord and turn the
power on.

When you turn on the power switch, the dispense

head and arm will travel the farthest X-Y distances
and then return to the “home” position at the left
rear of the X-Y matrix.

The fraction collector display will dim after 10

minutes if:

• you haven’t pressed a key

• a run has not been in progress
• power has not been turned off and then on

To brighten the display, press any key on the

keypad. The display will become lit, but the
function associated with the pressed key will not
be performed.

If you send a remote contact signal to the fraction

collector to start or end a run, this will brighten the
display and start or end the run. If you send a
remote GSIOC signal to the fraction collector, the
display will become lit and the function associated
with the signal will be performed.

2-21
Operation 3

The FC 203B Fraction Collector has a built-in front panel that you’ll use to set collection
mode and operating parameters. The 2-line display guides you through mode and
parameter selection using a menu format. The keys provide full operating control.

This section describes:

• the front panel

• how to start the fraction collector
• how to review and change collection options
• how to indicate collection into the same set or different set of tubes for each collection
cycle
• how to set collection options for each of the collection modes

3-1
 Operation 3
Front Panel

Front Panel
You give instructions to the fraction collector with
three kinds of keys: command hard keys, numeric
hard keys, and soft keys. You get information about
the 203B software with the HELP key.

Command Hard Keys

Hard keys are the labeled keys on the left side of
the front panel. These keys put you in control of the
fraction collector.

You can start or end a run, advance the dispense

head, drain the effluent, and edit the run options
with these keys. Use of the hard keys will be
described in detail as necessary.

You’ll recognize references to the hard keys

because they’re always written in CAPITAL letters.

Numeric Hard Keys

Parameter values are entered on the numeric
keypad at the right side of the fraction collector’s
front panel.

Also on this keypad are the NO and YES hard keys,

used to change or confirm parameter values.

Soft Keys
The four soft keys are located below the display.
Their functions vary as you perform different
operations.

It is the soft keys that make the fraction collector so

easy to use. At all times, you’ll know exactly which
choices you can make. Simply press the soft key
below the option you choose.

The examples in this manual illustrate the use of

the soft keys. You’ll recognize references to soft
keys because they’re always written in italicized
letters.

3-2
Operation 3

Front Panel
Help Key
The most useful hard key may be the HELP key. It’s
always available, to give you specific help and
instructions every step of the way.

To acquaint yourself with the HELP instructions,

press the HELP key any time during setup or
operation.

This will not disturb any run in progress.

3-3
 Operation 3
Start Up

Start Up
When all plumbing and electrical connections have
been made, turn on the fraction collector. The arm
and dispense head will start moving and the
display will read:

Homing Collection Head

FC 203B version X.XX

When the fraction collector “wakes up”, it

remembers and displays the last set of instructions it
was given, for example:

Time mode 1.00 min/tube

List Rack 0 Start

The fraction collector remembers:

• the last collection mode

• the most recent collection parameter values
• the last rack used

The fraction collector will automatically operate

according to the “wake-up” instructions.

Note: To review the options that apply in the

current mode, press the List soft key. If the options
displayed are appropriate, simply prepare your
vessels and press START. If the parameters need to
be adjusted, read the next section, Collection
Options.

Note: If a number greater than 0 was set for the

Tubes per cycle parameter (see Cycle Collection
section), a Cycle soft key appears instead of the
Start soft key.

3-4
Operation 3

Collection Options
Collection Options
Entering Numbers
Numbers enter at the far right of the display and
move to the left with each succeeding entry. The
decimal point, where applicable, doesn’t have to be
entered.

One easy way to change an entry is to press the

NO key to clear the current number. Then enter the
new number just as you’d write it on a piece of
paper.

For example, assume that two places are set to the

right of the decimal point. To enter the number 68,
clear the screen with the NO key. Then press “6”.
You will see:

0.06

Then press “8”. You will see:

0.68

Finally, press “0” twice. You will see:

68.00

Review or Change Options

To review the options that apply in the current
mode, press the List soft key. Options and their
current values are displayed one at a time.

If the displayed value is acceptable, press Ok. If you

want to change a value, simply enter new numbers
as described above.

3-5
 Operation 3
Collection Options

Review or Change Racks

To display a description of the current rack, press
the Rack soft key. You’ll see:

Rack code _ selected

[description of rack] Ok

To choose a different rack, enter the number code

for the new rack. The code for most racks is
imprinted on the rack.

From this display, press the HELP key to see a list

of all rack codes in the 203B’s memory.

Put the selected rack in place and press Ok.

Collection Into Same or New Tubes for

Each Cycle
You can have the fraction collector collect each
sample into the same set of tubes or into a new set
of tubes during each full collection cycle. If you
collect into the same set of tubes, each cycle begins
at tube 1. If you collect into a new set of tubes, the
cycle begins at the tube following the last tube of
the previous cycle.

To indicate which tubes are to be used during each

cycle, press EDIT, then 2. Then press the Next soft
key until you see:

Tubes per cycle = 0

Next Quit

If you want to collect each sample into the same set

of tubes, indicate 0 for the tubes per cycle.

If you want to collect each sample into a different

set of tubes, indicate a number greater than 0 for
the tubes per cycle.

For more information, refer to the section called

Cycle Collection.

3-6
Operation 3

Collection Options
Choosing Collection Modes
To choose a collection mode, press the EDIT key.
You’ll see:

Select an edit path:

Time Drop Peak Manl

Press the soft key corresponding to the mode of

choice.

For detailed descriptions of the collection modes,

refer to the section called Collection Modes.

Building Collection Windows

To build collection windows, follow the detailed
instructions in the section called Collection
Windows.

Ready to Start
Note: A change in the value of any parameter in
any mode is immediately updated in every mode!

If you are not the only user of the fraction collector,

check that the values you entered last have not
been changed by someone else.

When the options, rack, and collection mode are

acceptable and the display shows

___ mode
List Rack __Start

you’re ready to start. Pressing either the Start soft

key or the START key will start the collection.

Note: If a number greater than 0 was set for the

Tubes per cycle parameter (see Cycle Collection
section), a Cycle x soft key appears instead of the
Start soft key. The x identifies which cycle will be
started. To increment the cycle number, press the
Cycle x soft key.

3-7
 Operation 3
Collection Options

To start the run, press the START key at the left of

the display. When the run finishes, the cycle
number is incremented by 1. If the maximum cycle
number was just completed, then the cycle number
is set to 1.

If you press the END key during the run, the run
terminates and the cycle number is incremented by 1.

If you press the END key when the ready-to-run

display is being shown, the cycle number is set to 1.

3-8
Operation 3

During the Run

During the Run
Manual Override
During any run, you can use the ADV(ance),
DRAIN, and END keys to override any automatic
collection sequence.

When the ADV(ance) key is pressed, the dispense

head immediately moves to the next vessel. During
a run, the current vessel or fraction number is
always displayed.

DRAIN is a two-state key. Press it once and

effluent is diverted to the drain. Press it a second
time and the 203B resumes collection in the current
vessel. A yellow light on the DRAIN key indicates
that the 203B is draining.

While the 203B is draining, pressing ADV does not

resume collection. Instead it advances the number
of the current tube, which is always displayed
during a run. When DRAIN is pressed a second
time, the 203B collects into the tube shown on the
display.

Pressing END immediately ends the collection and

sends the dispense head to “home”.

Power Failure
In the case of a power failure during a run, the
203B will return to “home” and the display will
flash:

Warning: power failed

while collecting. -Ok-

This warns you that all effluent may not have been
collected.

Press Ok to confirm the message. The dispense

head returns to the “home” position. When the
dispense head returns “home”, it will not move
directly over the fractions that have already been
collected.

3-9
 Operation 3
Cycle Collection

Cycle Collection
The fraction collector enables you to collect
fractions two different ways:

• Collect each sample, or full collection cycle, into

the same set of tubes. During the run, each
cycle begins at tube 1. This collection type is
mostly used in preparative HPLC for collecting
purified fractions.

• Collect each sample, or full collection cycle, into

a different set of tubes. During the run, each
cycle begins at the tube following the last tube
of the previous cycle. This collection type is
mostly used in analytical HPLC.

Note: For automated cycle collection, you must

send a remote start signal to the 203B to begin each
cycle. See page 2-15 for information on making the
contact connection for remote start.

The Tubes per cycle parameter in the Technical

menu enables you to indicate which way you want
fractions collected. Using this parameter, you can
divide the rack into sections with a set number of
tubes per cycle. To display this parameter:

• Press EDIT, then 2.

• Press the Next soft key until you see:

Tubes per cycle = 0

Next Quit

3-10
Operation 3

Cycle Collection
Collect into Same Set Tubes
To collect each sample into the same set of tubes,
set the Tubes per cycle parameter to 0.

Example. If you set 0 as the number of tubes per

cycle for a Code 0 rack, each sample will be
collected into the same set of tubes per collection
cycle. Collection will be made into the following
tubes for each cycle.

Cycle Tubes
1 1–80
2 1–80
3 1–80
4 1–80
etc. etc.

Collect into New Set Tubes

To collect each sample into a new set of tubes, use
the fraction collector’s numeric keypad to indicate
a number greater than 0 for the Tubes per cycle
parameter. Using the tubes per cycle and the total
tube capacity of the rack, the 203B calculates the
number of cycles.

If the number of tubes can’t be divided into the

tube capacity evenly, the number of cycles is the
whole-number quotient; the remaining tubes are
not used.

Example 1. If you set 20 as the number of tubes per

cycle for a Code 0 rack, the number of cycles would
be 4. Collection will be made into the following
tubes for each cycle.

Cycle Tubes
1 1–20
2 21–40
3 41–60
4 61–80

3-11
 Operation 3
Cycle Collection

Example 2. If you set 25 as the number of tubes per

cycle for a Code 0 rack, the number of cycles would
be 3. Collection will be made into the following
tubes for each cycle.

Cycle Tubes
1 1–25
2 26–50
3 51–75

Ready-to-Run Display
When you set the tubes per cycle option, the Start
soft key changes to a Cycle x soft key on the ready-
to-run display. The x identifies which cycle will be
started. To increment the cycle number, press the
Cycle x soft key. By pressing the Cycle x soft key
again after the maximum cycle number, Cycle 1 can
be redisplayed.

To start the run, press the START key at the left of the
display. When the run finishes, the cycle number is
incremented by 1. If the maximum cycle number was
just completed, then the cycle number is reset to 1.

If you press the END key during the run, the run
terminates and the cycle number is incremented by 1.

If you press the END key when the ready-to-run

display is being shown, the cycle number is reset to 1.

Note: The tubes per cycle option cannot be used in

conjunction with the multiple collection head option.

If you try to set the multiple collection head option

when the tubes per cycle option is active, the 203B
prompts you to change the Tubes per cycle parameter
to 0.

If you try to set the tubes per cycle option when the
multiple collection head option is active, the 203B
prompts you to change the Multiple collect heads
parameter to single.

3-12
Operation 3

Collection Modes
Collection Modes
The fraction collector can collect fractions in several
ways.

Time mode

You can specify the collection time per fraction, in

hundredths of minutes.

Drop mode

You can specify the number of drops collected per

fraction.

Peak modes

If the fraction collector is connected to a detector,

you can instruct the 203B to collect only peaks. A
peak can be collected into many tubes. You decide
whether you want to subdivide a peak by drops or
time (minutes).

• peak + drop mode: You can specify the number

of drops dispensed in each tube.

• peak + time mode: You can specify the time per

tube.

In peak modes, you can also tell the 203B to collect

non-peak effluent by time or by drops.

Manual mode

You can collect fractions manually, giving you

complete freedom to advance the dispense head,
drain the effluent, or end the run.

Collection modes are set by pressing the EDIT key.

The next sections describe in detail how to set each
collection mode.

3-13
 Operation 3
Collection Modes

Choosing Time Collection Mode

Mode selection

Press the EDIT key.

You’ll see the mode selection display:

Select an edit path:

Time Drop Peak Manl

Press Time.

Time per tube selection

You’ll see the time selection display:

Time mode __ min/tube

Ok

Press the NO key to clear the display. Then enter

the desired time per tube from 0.01 to 99.99
minutes.

Press Ok.

Collection window option

You’ll see the collection window display:

Collect: Start to End

Ok Add
(If no windows have been built)

or

Collect: X.X to X.X.

Ok Add Del
(If windows have already been built)

Note: Collection windows let you specify time

intervals during which effluent is collected or
discarded. Refer to the section called Collection
Windows to build, add, or delete collection
windows.

Press Ok. Continue to press Ok if multiple collection

3-14 windows have been set up.
Operation 3

Collection Modes
Ready-to-run display

You’ll see the time mode ready-to-run display:

Time mode __ min/tube

List Rack __ Start

From this display, you can:

• review or change list of options (Press List)

• review or change current rack (Press Rack)
• start a collection run (Press Start)
• change the min/tube value (Enter the new
number)

Note: If Time had been the last mode selected, the

203B would wake up to this display.

Note: If a number greater than 0 was set for the

Tubes per cycle parameter (see Cycle Collection
section), a Cycle x soft key appears instead of the
Start soft key. The x identifies which cycle will be
started. To increment the cycle number, press the
Cycle x soft key.

To start the run, press the START key at the left of the
display. When the run finishes, the cycle number is
incremented by 1. If the maximum cycle number was
just completed, then the cycle number is reset to 1.

If you press the END key during the run, the run
terminates and the cycle number is incremented by 1.

If you press the END key when the ready-to-run

display is being shown, the cycle number is reset to 1.

3-15
 Operation 3
Collection Modes

Choosing Drop Collection Mode

Mode selection

Press the EDIT key.

You’ll see the mode selection display:

Select an edit path:

Time Drop Peak Manl

Press Drop.

Drop per tube selection

You’ll see the drop selection display:

Drop mode __ drop/tube

Ok

Press the NO key to clear the display. Then enter

the desired drops per tube from 1 to 9999 drops.

Press Ok.

Collection window option

You’ll see the collection window display:

Collect: Start to End

Ok Add
(If no windows have been built)

or

Collect: X.X to X.X.

Ok Add Del
(If windows have already been built)

Note: Collection windows let you specify time

intervals during which effluent is collected or
discarded. Refer to the section called Collection
Windows to build, add, or delete collection
windows.

Press Ok. Continue to press Ok if multiple collection

windows have been set up.
3-16
Operation 3

Collection Modes
Ready-to-run display

You’ll see the drop mode ready-to-run display:

Drop mode __ drop/tube

List Rack __ Start

From this display, you can:

• review or change list of options (Press List)

• review or change current rack (Press Rack)
• start a collection run (Press Start)
• change the drop/tube value (Enter the new
number)

Note: If Drop had been the last mode selected, the

203B would wake up to this display.

Note: If a number greater than 0 was set for the

Tubes per cycle parameter (see Cycle Collection
section), a Cycle x soft key appears instead of the
Start soft key. The x identifies which cycle will be
started. To increment the cycle number, press the
Cycle x soft key.

To start the run, press the START key at the left of the
display. When the run finishes, the cycle number is
incremented by 1. If the maximum cycle number was
just completed, then the cycle number is reset to 1.

If you press the END key during the run, the run
terminates and the cycle number is incremented by 1.

If you press the END key when the ready-to-run

display is being shown, the cycle number is reset to 1.

3-17
 Operation 3
Collection Modes

Choosing Peak + Time Collection

Mode
Gilson strongly recommends using a 3-way valve
when operating the 203B in this collection mode.
Refer to Section 2 for instructions on installing a
3-way valve.

Mode selection

Press the EDIT key.

You’ll see the mode selection display:

Select an edit path:

Time Drop Peak Manl

Press Peak.

Fractionation selection

You’ll see:

Subfractionate peaks by:

Time Drop Quit

To fractionate each peak into several tubes on the

basis of time per tube, press Time.

Time per tube selection

You’ll see the time per tube selection display:

Peak mode __min/tube

Ok

Press the NO key to clear the display. Then enter

the desired time per tube from 0.01 to 99.99
minutes.

If you want to collect each peak into a single

tube, set a high value that will not overflow the
tube (e.g., 30.00 min/tube).

Press Ok.

3-18
Operation 3

Collection Modes
Non-peak collection option

You’ll see either of two displays:

Nonpeak: __ min/tube
Ok Discard
(To collect non-peak fractions)

or

Nonpeak: discard (time)

Ok Collect
(If non-peaks will be discarded)

You can choose whether to discard or to collect the

effluent between peaks by toggling the Discard/
Collect soft key. The current selection is noted on
the top line of the display.

If you choose to collect non-peak effluent, enter the

non-peak collection time per tube using the numeric
keypad.

When finished, press Ok.

Setting peak parameters

Before you can perform a peak collection, you must

run a preliminary sample to collect information
that helps the fraction collector determine when
and where to look for peaks.

This involves a little extra effort from you, but that

effort will be offset by improved results. You’ll
collect purer peaks and even collect overlapping
peaks into separate tubes.

3-19
 Operation 3
Collection Modes

Peak width display

The peak width is measured at 1/2 of a peak’s

maximum height. Measure the peak width on a
peak of average width. This will ensure that
narrower and wider peaks will also be collected.
Use the X-axis scale to measure average peak
width.

The peak width display looks like this:

Peak width: __ min

Ok

Enter the value in minutes. Then press Ok.

Peak height display or peak level display

If the Collect by slope option is selected in the

Technical menu, you’ll be prompted for the
minimum height of peaks to collect. Peak height
enables the 203B to distinguish peaks from noise.
From the millivolt (bottom to top) scale, determine
the height (in millivolts) of the smallest peak you
wish to collect. Use the Y-axis scale to measure
minimum peak height.

The peak height display looks like this:

Peak height: __ mV
Ok

Enter the value in millivolts (mV). Then press Ok.

If you selected the Collect peaks by level option in

the Technical menu, the display prompts you for a
millivolt (mV) level:

Peak level: __ mV
Ok

Peaks above the indicated mV value will be

collected while peaks below this value will be
discarded. Use the Y-axis scale to identify the
absolute threshold level.

3-20
Operation 3

Collection Modes
If you have a relatively stable baseline, set the
level value at about 2 times the expected baseline
fluctuation. For example, if the baseline stays
between 0 and 1 mV, set the level at 2 mV.

Appendix C describes the options in the Technical

menu. Appendix H contains more information on
peak detection by slope or mV level.

Delay time

You’ll see:

Delay time: __ min

Ok

Delay time is the time required for liquid to move

from the flow cell of the detector to the dispense
head of the 203B. Tube advancement will be
delayed by this amount of time, optimizing the
collection of pure peaks. Delay time is more critical
in analytical HPLC than in large-scale prep LC.

To calculate delay time, you’ll need to know:

• flow rate (mL/min.)

• length of detector-to-collector tubing (cm)
• volume of inlet tubing from detector (µL/in.)

Find the volume of commonly used tubing in the

following table:

Internal Diameter Volume

in. mm µL/in.
0.010 0.25 1.29
0.020 0.50 5.15
0.030 0.75 11.58
0.040 1.00 20.59

You can calculate delay time (minutes) with this

formula:

Delay time = (length x volume)/flow rate

After entering the delay time value, press Ok.

3-21
 Operation 3
Collection Modes

Collection window option

You’ll see the collection window display:

Collect: Start to End

Ok Add
(If no windows have been built)

or

Collect: X.X to X.X.

Ok Add Del
(If windows have already been built)

Note: Collection windows let you specify time

intervals during which effluent is collected or
discarded. Refer to the section called Collection
Windows to build, add, or delete collection
windows.

Press Ok. Continue to press Ok if multiple collection

windows have been set up.

Ready-to-run display

You’ll see the peak + time mode ready-to-run display:

Peak mode __ min/tube

List Rack __ Start

From this display, you can:

• review or change list of options (Press List)

• review or change current rack (Press Rack)
• start a collection run (Press Start)
• change the min/tube value (Enter the new
number)

Note: If peak + time had been the last mode

selected, the fraction collector would wake up to
this display.

3-22
Operation 3

Collection Modes
Note: The 203B has been preset to detect positive
peaks. If your application requires detection of both
positive and negative peaks, you may select that
option via the Technical menu. See Appendix C for
information.

Note: If a number greater than 0 was set for the

Tubes per cycle parameter (see Cycle Collection
section), a Cycle x soft key appears instead of the Start
soft key. The x identifies which cycle will be started.
To increment the cycle number, press the Cycle x soft
key.

To start the run, press the START key at the left of the
display. When the run finishes, the cycle number is
incremented by 1. If the maximum cycle number was
just completed, then the cycle number is reset to 1.

If you press the END key during the run, the run
terminates and the cycle number is incremented by 1.

If you press the END key when the ready-to-run

display is being shown, the cycle number is reset to 1.

3-23
 Operation 3
Collection Modes

Choosing Peak + Drop Collection

Mode
Gilson strongly recommends using a 3-way valve
when operating the fraction collector in this
collection mode. Refer to Section 2 for instructions
on installing a 3-way valve.

Mode selection

Press the EDIT key.

You’ll see the mode selection display:

Select an edit path:

Time Drop Peak Manl

Press Peak.

Fractionation selection

You’ll see:

Subfractionate peaks by:

Time Drop Quit

To fractionate each peak into several tubes, on the

basis of number of drops per tube, press Drop.

Drop per tube selection

You’ll see the drop per tube selection display:

Peak mode ___ drop/tube

Ok

Press the NO key to clear the display. Then enter

the desired drops per tube from 1 to 9999 drops.

If you want to collect each peak into a single

tube, set a higher value than the number of drops
contained in a peak.

Press Ok.

3-24
Operation 3

Collection Modes
Non-peak collection option

You’ll see either of two displays:

Nonpeak: __ drop/tube
Ok Discard
(To collect non-peak fractions)

or

Nonpeak: discard (drop)

Ok Collect
(If non-peaks will be discarded)

You can choose whether to discard or to collect the

effluent between peaks by toggling the Discard/
Collect soft key. The current selection is noted on
the top line of the display.

If you choose to collect non-peak effluent, enter the

non-peak collection time per tube using the
numeric keypad.

When finished, press Ok.

Setting peak parameters

Before you can perform a peak collection, you must

run a preliminary sample to collect information
that helps the fraction collector determine when
and where to look for peaks.

This involves a little extra effort from you, but that

effort will be offset by improved results. You’ll
collect purer peaks and even collect overlapping
peaks into separate tubes.

3-25
 Operation 3
Collection Modes

Peak width display

The peak width is measured at 1/2 of a peak’s

maximum height. Measure the peak width on a
peak of average width. This will ensure that
narrower and wider peaks will also be collected.

Use the X-axis scale to measure average peak

width.

The peak width display looks like this:

Peak width: __ min

Ok

Enter the value in minutes. Then press Ok.

Peak height display or peak level display

If the Collect by slope option is selected in the

Technical menu, you’ll be prompted for the
minimum height of peaks to collect. Peak height
enables the fraction collector to distinguish peaks
from noise. From the millivolt (bottom to top) scale,
determine the height (in millivolts) of the smallest
peak you wish to collect. Use the Y-axis scale to
measure minimum peak height.

The peak height display looks like this:

Peak height: __ mV
Ok

Enter the value in millivolts (mV). Then press Ok.

If you selected the Collect peaks by level option in

the Technical menu, the display prompts you for a
millivolt (mV) level:

Peak level: __ mV
Ok

Peaks above the indicated mV value will be collected

while peaks below this value will be discarded. Use
the Y-axis scale to identify the absolute threshold
level.

3-26
Operation 3

Collection Modes
If you have a relatively stable baseline, set the
level value at about 2 times the expected baseline
fluctuation. For example, if the baseline stays
between 0 and 1 mV, set the level at 2 mV.

Appendix C describes the options in the Technical

menu. Appendix H contains more information on
peak detection by slope or mV level.

Delay time

You’ll see:

Delay time: __ min

Ok

Delay time is the time required for liquid to move

from the flow cell of the detector to the dispense
head of the 203B. Tube advancement will be
delayed by this amount of time, optimizing the
collection of pure peaks. Delay time is more critical
in analytical HPLC than in large-scale prep LC.

To calculate delay time, you’ll need to know:

• flow rate (mL/min.)

• length of detector-to-collector tubing (cm)
• volume of inlet tubing from detector (µL/in.)

Find the volume of commonly used tubing in the

following table:

Internal Diameter Volume

in. mm µL/in.
0.010 0.25 1.29
0.020 0.50 5.15
0.030 0.75 11.58
0.040 1.00 20.59

You can calculate delay time (minutes) with this

formula:

Delay time = (length x volume)/flow rate

After entering the delay time value, press Ok.

3-27
 Operation 3
Collection Modes

Collection window option

You’ll see the collection window display:

Collect: Start to End

Ok Add
(If no windows have been built)

or

Collect: X.X to X.X.

Ok Add Del
(If windows have already been built)

Note: Collection windows let you specify time

intervals during which effluent is collected or
discarded. Refer to the section called Collection
Windows to build, add, or delete collection
windows.

Press Ok. Continue to press Ok if multiple collection

windows have been set up.

Ready-to-run display

You’ll see the peak + drop mode ready-to-run

display:

Peak mode __ drop/tube

List Rack __ Start

From this display, you can:

• review or change list of options (Press List)

• review or change current rack (Press Rack)
• start a collection run (Press Start )
• change the drop/tube value (Enter the new
number).

Note: If peak + drop had been the last mode

selected, the 203B would wake up to this display.

3-28
Operation 3

Collection Modes
Note: The 203B has been preset to detect positive
peaks. If your application requires detection of both
positive and negative peaks, you may select that
option via the Technical menu. See Appendix C for
information.

Note: If a number greater than 0 was set for the

Tubes per cycle parameter (see Cycle Collection
section), a Cycle x soft key appears instead of the Start
soft key. The x identifies which cycle will be started.
To increment the cycle number, press the Cycle x soft
key.

To start the run, press the START key at the left of the
display. When the run finishes, the cycle number is
incremented by 1. If the maximum cycle number was
just completed, then the cycle number is reset to 1.

If you press the END key during the run, the run
terminates and the cycle number is incremented by 1.

If you press the END key when the ready-to-run

display is being shown, the cycle number is reset to 1.

3-29
 Operation 3
Collection Modes

Choosing Manual Collection Mode

Mode selection

Press the EDIT key.

You’ll see the mode selection display:

Select an edit path:

Time Drop Peak Manl

Press Manl.

Delay time option

You’ll see the delay time display:

Delay time:____ min

Ok

Delay time is the time required for liquid to move

from the flow cell of the detector to the dispense
head of the 203B. Tube advancement will be
delayed by this amount of time, optimizing the
collection of pure peaks. Delay time is more critical
in analytical HPLC than in large-scale prep LC.

If needed, calculate the delay time as described for

the peak modes. Press the NO key to clear the
display. Then enter the desired delay time.

Press Ok.

3-30
Operation 3

Collection Modes
Collection window option

You’ll see the collection window display:

Collect: Start to End

Ok Add
(If no windows have been built)

or

Collect: X.X to X.X.

Ok Add Del
(If windows have already been built)

Note: Collection windows let you specify time

intervals during which effluent is collected or
discarded. Refer to the section called Collection
Windows to build, add, or delete collection
windows.

Press Ok. Continue to press Ok if multiple collection

windows have been set up.

Ready-to-run display

You’ll see the manual mode ready-to-run display:

Manual mode
List Rack __ Start

From this display, you can:

• review or change list of options (Press List)

• review or change current rack (Press Rack)
• manually start and stop the run

Note: If Manual had been the last mode selected,

the fraction collector would wake up to this
display.

3-31
 Operation 3
Collection Modes

Delayed advance function

The manual mode of the fraction collector provides

a special delayed advance function.

During a run in manual mode, you’ll see:

Manual mode Delayed

Tube Adv

Pressing the Delayed Adv soft key delays tube

advance according to the delay time value entered.

The ADV key, on the other hand, causes an

instantaneous advance.

The manual mode of the fraction collector can

operate semiautomatically, if you take advantage of
the collection windows and delayed advance
capabilities.

However, you always have complete manual

control in this mode. Just press START, ADV,
DRAIN or END at the appropriate times. Make
sure you understand the ADV/DRAIN interplay,
described on page 3-9, before doing so.

3-32
Operation 3

Collection Windows
Collection Windows
Gilson strongly recommends using a 3-way valve
when operating the fraction collector in this
collection mode. Refer to Section 2 for instructions
on installing a 3-way valve.

Many users ask a fraction collector to collect a

certain number of drops per tube or to collect each
fraction for a defined length of time. Most of these
users want to save every fraction, that is, to collect
from start to end.

On the other hand, some users may know that

during a particular time interval, no fractions of
interest will elute from their column.

With the FC 203B Fraction Collector, you can

define time intervals during which the effluent is
either collected in fractions or is discarded. These
time intervals are called collection windows.

When the fraction collector is inside a collection

window, fractions are collected.

When the fraction collector is outside a collection

window, the dispense head will move over the
drain trough and divert the effluent to waste.
When using a 3-way valve, the effluent will be
diverted to waste via the valve.

Windows are universal

Collection windows apply in all modes: time, drop,

peak + time, peak + drop, and manual.

If you select a new collection mode, the windows

built in a previous mode remain active in the new
mode. If you don’t want to use those windows in
the new mode, delete them.

3-33
 Operation 3
Collection Windows

Examples

Example 1. Start to end collection window

If you collect from start to end, your entire run is

one long collection window, starting at time 0 and
ending when the last fraction is collected.

Example 2. One open-ended window

If you know that nothing of interest will elute

within the first 5 minutes of a run, you could
instruct the fraction collector to drain the column
effluent for 5 minutes before collecting fractions.

Example 3. Two windows

If you are collecting fractions for 0.5 minutes per

tube and want to collect a peak which elutes at 4
minutes and another which elutes at 15 minutes,
you could instruct the fraction collector to:

windows
drain for 3 minutes

collect 0.5 minute fractions

for 2 minutes (3–5 min.)

drain for 9 minutes

collect 0.5 minute fractions

for 2 minutes (14–16 min.)

3-34
Operation 3

Collection Windows
Example 4. Two windows

If you are collecting fractions that contain 25 drops

per tube and want to collect a peak which elutes at
4 minutes and another which elutes at 15 minutes,
you could instruct the fraction collector to:

windows
drain for 3 minutes

collect 25-drops-per-tube fractions

for 2 minutes (3–5 min.)

drain for 9 minutes

collect 25-drops-per-tube fractions

for 2 minutes (14–16 min.)

Note: You may have noticed from Examples 3 and

4 that a collection window specifies a time interval,
in any automatic mode, during which you can
collect fractions either by length of time or by drop
number.

The next section describes how to build windows to

suit your collection needs.

3-35
 Operation 3
Collection Windows

Building No Windows

Collection window display

The collection window display looks like this:

Collect: Start to End

Ok Add

Start refers to 0 minutes, the beginning of each run.

End refers to the last tube in a rack. It is represented
by 999.9 min.

If you want to collect every fraction from Start to

End, just press the Ok soft key.

Building One Window

If you want to collect only a portion of the total
effluent, build a collection window to specify when
you want to collect.

The most common situation that requires a collection

window is a single wait period at the beginning of a
run. Suppose you want to discard the effluent for
the first 2 minutes of a run.

Note: The time resolution when building collection

windows is 0.1 minute.

Collection window display

The collection window display will read:

Collect: Start to End

Ok Add

To add a collection window, press Add. You’ll see:

Collect from: 0.0

Ok

Press 2, then 0 (Remember the fixed decimal point!).

Then press Ok. You’ll see:

Collect: 2.0 to 999.9

Ok
3-36
Operation 3

Collection Windows
The End of a run is represented by 999.9 min.

Press Ok. You’ll see:

Collect: 2.0 to End

Ok Add Del

Press Ok. This window tells the 203B to discard the

effluent for 2 minutes before it starts to collect
fractions.

Building More Than One Window

You can build up to 10 collection windows into a
run.

Suppose you want to:

• discard the effluent for the first 5 minutes of a

run
• then collect fractions for the next 5 minutes
• then discard for 10 minutes
• then collect until the end of the run

Collection window display

The collection window display will read:

Collect: Start to End

Ok Add

To build the first collection window, press Add.

You’ll see:

Collect from: 0.0

Ok

Using this display, set the start time for the first
collection window. Press 5, then 0 (Remember the
fixed decimal point!). Then press Ok. You’ll see:

Collect: 5.0 to 999.9

Ok

3-37
 Operation 3
Collection Windows

Using this display, set the end time for the first
collection window. Press 1, 0, 0. Then press Ok.
You’ll see:

Collect: 5.0 to 10.0

Ok Add Del

This window tells the fraction collector to collect

fractions between 5 and 10 minutes of the run.

To build the second collection window, press Add.

You’ll see:

Collect from: 0.0

Ok

Note: Your first collection window (5.0 to 10.0

minutes) has not been deleted! You’ll always see 0.0
when you build a new window. Existing windows
are not affected.

Press 2, 0, 0 (Remember the fixed decimal point!).

Then press Ok. You’ll see:

Collect: 20.0 to 999.9

Ok

Since you want to collect fractions until the end of

the run (represented by 999.9 minutes), press Ok.

You’ll see a review of the first window you built:

Collect: 5.0 to 10.0

Ok Add Del

Press Ok. You’ll see the second window:

Collect: 20.0 to End

Ok Add Del

Press Ok.

The review displays each collection window,

organized from start to end of the run.

If you’re building more than two collection windows,

write them down first. You don’t have to build in
order, but doing so will simplify the process for you.
3-38
Operation 3

Collection Windows
Deleting Windows
If you don’t want a window that is displayed, press
Del.

To delete all collection windows, keep pressing Del

until you see:

Collect: Start to End

Ok Add

Nonsense Windows
The fraction collector won’t let you build the two
types of nonsense windows described below.

Overlapped time

A window cannot start before another has ended.

If you tried to add a third window to the previous
example (e.g., collect from 25 to 30 minutes), you
would see:

Overlapped time. Quit

To add the third collection window:

• press Quit
• press Ok until the Collect 20.0 to End window
appears
• press Del to delete that window
• press Add and build the 25 to 30 minute
window

Backward time

The end time in a window must be later than the

start time. If you tried to start collection at 2
minutes and end at 1 minute of the run, you would
see:

Backward times. Quit

Press Quit and rebuild the window.

3-39
Maintenance 4

The fraction collector is designed for reliable, long term use in normal laboratory and cold-
room environments. Simple care and maintenance will ensure proper operation for many
years.

4-1
 Maintenance 4
Cold-Room Use

Cold-Room Use
You can leave the fraction collector in a cold-room,
provided that the power is left on continuously.
Leaving the power on provides enough internal heat
to prevent condensation from forming on critical
components.

4-2
Maintenance 4

Chemical Compatibility
Chemical Compatibility
The case and keypad of the fraction collector are
resistant to most solvents and buffers used in the
lab. Follow good laboratory technique by cleaning
spills with either water or alcohol.

You can easily remove the rack, pan, and drain

trough for cleaning.

If you have questions concerning the chemical

resistance of the 203B, contact your regional Gilson
representative or call Gilson directly at 800-445-7661
or 608-836-1551. You can also contact Gilson
Customer Service via its e-mail address:
[email protected]

Outside the U.S., contact your Gilson representative.

4-3
 Maintenance 4
Transporting the Fraction Collector

Transporting the Fraction

Collector
When moving the fraction collector to another
location, do not use the arm as a handle. Doing so
may damage the unit or cause misalignment of the
X-Y positioning. You may carry the fraction collector
using the large rectangular opening in the front of
the unit.

4-4
Maintenance 4

Replacing a Fuse
Replacing a Fuse
To change a fuse, follow these steps.

1 Disconnect the power cord from the power

outlet and from the rear panel receptacle.

2 Locate the fuse drawer on the rear panel.

3 Insert a small screwdriver into the notch

located to the right of the fuse drawer.

4 Twist the screwdriver to open and remove the

fuse drawer. The fuse drawer contains one fuse
for a 100/120 voltage selection. It contains two
fuses for a 220/240 voltage selection.

5 Remove the old fuse(s) and insert the new

fuse(s).

You must use “T” type fuses. The fuses that you
install depend on the voltage of your unit.

Voltage selection Fuse value Fuse size

100/120V 0.5 amp 5 mm x 20 mm
220/240V 0.25 amp 5 mm x 20 mm

6 Insert the fuse drawer into its receptacle in the

fraction collector.

A blown fuse may indicate the existence of

another problem in the instrument. If the
replacement fuses blow, don’t try others.
Contact your local representative or Gilson. See
the Before calling us section on page 5-7.

4-5
Troubleshooting 5

If you encounter a problem while operating the FC 203B Fraction Collector, refer to the
following pages. If you cannot solve or isolate the problem, contact the Gilson Customer
Service Department or your local Gilson representative. See Before calling us on page 5-7.

5-1
 Troubleshooting 5
Troubleshooting

Troubleshooting
Machine makes loud noise

Connect the fraction collector to a grounded outlet.

The stepper motor and microprocessors in the

fraction collector must establish a reference point
(“home” position) each time the unit is turned on.
“Home” position is at the left rear.

It is normal for the fraction collector to make a

vibrating noise for a few seconds while the motors
are “homing.” This should occur only after the unit
is turned on or when power is restored after a power
failure. Minimize the noise by manually pushing the
XY arm to the far left and the dispense head to the
rear before turning the unit on.

Unit makes loud noise when changing X or Y

position in run

Check for obstruction to X or Y movement by

turning off the fraction collector and moving the
dispense head and XY arm. They should move
smoothly.

Drops are missing the vessel

Check to be sure rack code in program matches rack

you are using.

Check rack location. Rack may be out of position on

tray or locator base.

Check for a crimp in effluent tubing. Replace tubing

into dispense head.

Check that tubing in the dispense head’s drop

detector is not bent.

Check mechanical XY arm assembly for alignment.

Turn the unit off and then restart it. If still out of
alignment, contact your local representative or the
Gilson Customer Service Department.

5-2
Troubleshooting 5

Troubleshooting
Drop detector not counting drops, or
unreliable counting

Check that tubing in the dispense head’s drop

detector is not bent.

Check that tubing in the dispense head’s drop

detector is properly gauged. See page E-2.

Be sure the inlet tubing fitting is completely

tightened so the tubing cannot move up and down.

Clean glass collar in drop detector (accessible from

underside of dispense head; you will have to
remove test tube rack). Use a cotton swab moistened
with alcohol or water.

Replace glass collar in drop detector if unable to

clean existing collar. Remove two small screws from
underside of dispense head. Lift top piece off
bottom of dispense head. Lift out glass collar and
install replacement. Be careful not to break the wires
connected to the dispense head.

Replace drop detector assembly (part number

170350V) if faulty. Instructions on how to replace
this assembly are supplied with the replacement.

Replace electrical chassis.

5-3
 Troubleshooting 5
Troubleshooting

Separation problems in peak mode

Check peak parameters entered in the software.

When collecting peaks by slope, peak height should
be mV height of smallest peak. When collecting peaks
by level, peak level should be mV value above which
to collect peaks. Peak width should be average width
of peaks at half height in chromatogram.

Specify negative peaks in the Technical menu (press

EDIT 2 on keypad).

Event mark on UV trace interfering with peak

detection. Connect event mark to recorder or data
system rather than to event input of detector.

Bubbles in mobile phase creating spikes on recorder

trace; degas mobile phase or eliminate plumbing
leak that is causing bubbles.

Check that delay time (time for peak material to

travel from detector to fraction collector) is set
correctly.

Not recognizing peaks in peak mode

Check detector input voltage. Should be less than

100 mV full scale.

Check peak parameters.

Check polarity. Connections from detector output to

fraction collector input may be incorrect.

5-4
Troubleshooting 5

Troubleshooting
3-way valve not functioning or functioning
incorrectly

Check connection to 3-pin socket of transition

printed circuit board, accessible through XY arm
opening. Plugging or unplugging valve with power
on is not suggested as this may cause machine to
reset.

Check that connector is oriented properly.

Check fluid connections to valve for proper

orientation.

Contact your local Gilson representative or the

Gilson Customer Service Department. Valve may be
defective.

Input functions not operating

Make sure connections into terminal block connector

are secure.

Make sure terminal block connector is secure in

multipurpose I/O port.

Check connections for proper pin assignments.

Be sure pins from external devices are assigned

correctly.

Check polarity of input. Inputs should be a contact

closure. If not, it must be TTL level (logic 0
activates).

Confirm that source supplying input to the fraction

collector is working.

5-5
 Troubleshooting 5
Troubleshooting

Output functions not operating

Make sure connections into terminal block

connector are secure.

Make sure terminal block connector is secure in

multipurpose I/O port.

Check connections for proper pin assignments.

Output from the fraction collector should be

compatible with the device to which it is interfaced.
Outputs are contact closures.

Note: Output 2 mode is programmable in the

Technical menu (press EDIT 2 on keypad).

Unit not operational

Make sure power is turned on.

Check AC power cord connections.

Try different AC outlet.

Check fuse(s); replace if necessary.

Replace electrical chassis. Contact your local

representative or the Gilson Customer Service
Department.

Unit blows fuses

Contact your local representative or the Gilson

Customer Service Department.

5-6
Troubleshooting 5

Repair and Return Policies

Repair and Return Policies
Before calling us

Gilson Customer Service personnel will be able to

serve you more efficiently if you have the following
information:

• serial number and model number of the devices

involved. The serial number is on the back face
of the 203B under the pan.
• installation procedure you used
• list of concise symptoms
• list of operating procedures and conditions you
were using when the problem arose
• list of other devices connected to the 203B and a
description of those connections
• list of other electrical connections in the room

Warranty repair

Units covered under warranty will be repaired and

returned to you at no charge. If you have any
questions about applicability, please contact Gilson
or your authorized representative.

Non-warranty repair

For out-of-warranty repairs, contact your local

Gilson representative or the Gilson factory. A
Customer Service representative will discuss service
options with you and can assist in making
arrangements to return the equipment, if necessary.

Rebuilt exchange

For some units, rebuilt exchange components are

available. Contact Gilson for details.

5-7
 Troubleshooting 5
Repair and Return Policies

Return procedure

In the United States, contact the Gilson Customer

Service Department to obtain authorization before
returning any Gilson equipment. To return a piece
of equipment:

• Carefully pack the unit to prevent damage in

transit. Check with Gilson regarding proper
method of shipment. No responsibility is
assumed by Gilson for damage caused by
improperly packaged instruments. Indicate the
authorization on the carton and on the packing
slip.

• Always insure for the replacement value of the

unit.

• Include a description of symptoms, your name,

address, phone number and purchase order to
cover repair costs, return and shipping charges,
if your institution requires it. Ship to:

Gilson, Inc.
Attention: Customer Service
(indicate the authorization here)
3000 W. Beltline Highway
Middleton, WI 53562

Outside the United States, contact your Gilson

representative for return procedures.

5-8
Racks A

The FC 203B Fraction Collector accommodates many racks, all described in this appendix.
Each rack has its own code number, and most of the racks have their code numbers
imprinted on them.

The fraction collector always needs to know the code number for the rack you’re using so
it can accurately track the vessel positions for that rack. The diagram below shows the
collection pattern used for almost all FC 203B racks. The only exceptions are:

• Code 15 rack with microplate (see page A-5)

• Code 29 LE and 29 SE racks when used with capped Eppendorf vials (see pages A-7
and A-8)

Three racks (Code 30, 32, and 33) can maintain your fractions at a constant temperature.
Tubes are placed in an aluminum block that is surrounded by a flowing water bath. To
use these thermostated racks, you’ll have to order:

• thermostating cuvette (part number 2704429)

• special rack holder for thermostated racks (part number 2704471). You will not need
this rack holder when you use a Code 32 rack with 100 mm tubes.

Collection Pattern
A-1
 Appendix A
Racks

Collection plate

For 80 vessels or direct collection into 80 cavities

Material: polypropylene
Cavity capacity: 2.5 mL/cavity
Vessels: 11 x 40 mm (1.5 mL) Eppendorf vials
without caps
Part number: 12240102, package of 20
Note: When setting up parameters, identify the
collection plate as rack code 0 (zero).

Code 0 rack

For 80 vessels
Material: polypropylene
Vessels and capacity: 12 x 32 mm vials (2 mL)
Part number: 270430

Code 1 rack

For 80 vessels
Material: polypropylene
Vessels and capacity: 13 x 65 mm tubes (7 mL)
13 x 100 mm tubes (9 mL)
Part number: 12240101, package of 6

Code 2 rack

For 119 vessels

Material: polypropylene
Vessels and capacity: 6 x 50 mm tubes (1 mL)
Part number: 130402
Note: Gilson recommends the use of a 3-way valve
and small bore needle with this rack.

A-2
Appendix A

Racks
Installation instructions for Code 4, Code 11
and Code 14 racks

1 Fill the rack with the appropriate collection

tubes. (See page A-4.)

2 These racks each contain a pair of metal

alignment tabs as illustrated below. Slide the
rack onto the rack holder, keeping the
alignment tabs ABOVE the antlers.

3 Firmly position the alignment tabs on the

horizontal part of the antlers as shown below.

A-3
 Appendix A
Racks

Code 4 rack

For 80 vessels
Material: stainless steel
Vessels and capacity: 12 x 75 mm tubes (5 mL)
Part number: 130412
Installation: see page A-3

Code 11 rack

For 105 vessels

Material: stainless steel
Vessels and capacity: 13 x 100 mm (9 mL)
Part number: 170413
Installation: see page A-3

Code 14 rack

For 128 vessels

Material: stainless steel
Vessels and capacity: 12 x 75 mm tubes (5 mL)
Part number: 170414
Installation: see page A-3

A-4
Appendix A

Racks
Code 15 rack holder

For a standard or deep-well microplate

Material: aluminum
Vessel capacity: 0.3 mL/well
Part number: 170415
Installation: Do one of the following.

• If you are using a standard microplate, align

the microplate holder with the upper set of
holes on the front standoffs of the fraction
collector. Attach the holder using thumbscrews.
Place the microplate onto the holder.

• If you are using a deep-well microplate, align

the microplate holder with the lower set of
holes on the front standoffs of the fraction
collector. Attach the holder using thumbscrews.
Place the microplate onto the holder.

Collection pattern: Refer to diagrams and their

related text.

Indicate rack code “15” Indicate rack code “17”

in the software if you in the software if you
want to collect from want to collect in a
left to right serpentine pattern

Note: A 3-way valve and small bore needle are

required for collection into a microplate.

A-5
 Appendix A
Racks

Code 20 rack

For 108 vessels

Material: polypropylene
Vessels and capacity: 10 x 100 mm tubes (4.5 mL)
Part number: 150425

Code 21 rack

For 60 vessels
Material: polypropylene
Vessels and capacity: 13 x 100 mm tubes (9 mL)
Part number: 150422

Code 23 rack

For 44 vessels
Material: polypropylene
Vessels and capacity: 17 x 55 mm vials (6.8 mL)
17 x 65 mm vials (8 mL)
Part number: 150426

Code 23W rack

For 44 vessels
Material: polypropylene and stainless steel
Vessels and capacity: Waters WISP vials (4 mL)
Part number: 270433
Note: When setting parameters in the software,
indicate rack code “23”.

A-6
Appendix A

Racks
Code 24 rack

For 14 vessels
Material: polypropylene
Vessels and capacity: 28 x 60 mm (20 mL)
scintillation vials
Part number: 150427

Code 28 rack

For 108 vessels

Material: polypropylene
Vessels and capacity: 10 x 65 mm tubes (3 mL)
10 x 75 mm tubes (4 mL)
Part number: 150420

Code 29 rack

For 60 vessels
Material: polypropylene
Vessels and capacity: 12 x 65 mm tubes (3.5 mL)
12 x 75 mm tubes (5 mL)
Part number: 150429

Code 29 LE rack

For 60 large (1.5 mL) Eppendorf vials

Material: polypropylene and stainless steel
Vessels and capacity: 11 x 40 mm (1.5 mL)
Part number: 2704342
Note: When setting parameters in the software,
indicate rack code “29”.

A-7
 Appendix A
Racks

Code 29 SE rack

For 60 small (0.5 mL) Eppendorf vials

Material: polypropylene and stainless steel
Vessels and capacity: 7 x 30 mm (0.5 mL)
Part number: 2704341
Note: When setting parameters in the software,
indicate rack code “29”.

Using capped Eppendorf vials in Code 29 LE

or 29 SE rack

Place the vials in the center two rows with the caps
facing outward. When setting parameters in the
software, indicate rack code “19”.

A-8
Appendix A

Racks
Thermostated Racks
To use the thermostated racks described below,
you’ll have to order:

• thermostating cuvette (part number 2704429)

• special rack holder for thermostated racks (part
number 2704471). You will not need this rack
holder when you use a Code 32 rack with 100
mm tubes.

Code 30 rack

Thermostated rack for 60 vessels

Material: aluminum
Vessels and capacity: 12 x 32 mm vials (2 mL)
Part number: 2704430

Code 32 rack

Thermostated rack for 60 vessels

Material: aluminum
Vessels and capacity: 13 x 65 mm tubes (7 mL)
13 x 100 mm tubes (9 mL)
Part number: 2704432
Note: When using a Code 32 rack with 13 x 65 mm
tubes, set rack code to 30 in the software.

Code 33 rack

Thermostated rack for 14 vessels

Material: aluminum
Vessels and capacity: 28 x 60 mm vials (20 mL)
Part number: 2704433

A-9
Parameter Review Menu B

When you store parameters within any fraction collection mode, those parameters are
active in every mode. For example, a collection window established in drop mode is also
active in time mode.

If your fraction collector has several users, you must take care to review all parameters
before beginning a run.

B-1
 Appendix B
Parameter Review Menu

The fraction collector provides a convenient list of

parameters and current values for review. You can
also change values from this menu. The new values
you enter here will apply whenever that parameter
applies.

To access this menu, press EDIT, then 1. You’ll see,

in order:

Time mode __ min/tube

Ok

Drop mode __ drop/tube

Ok

Peak mode __ min/tube

Ok

Peak mode __ drop/tube

Ok

Nonpeak: discard (time)

Ok Collect
or
Nonpeak: __ min/tube
Ok Discard

Nonpeak: discard (drop)

Ok Collect
or
Nonpeak: __ drop/tube
Ok Discard

Peak width: _ min

Ok

Peak height: __ mV
Ok
or
Peak level: __ mV
Ok

Delay time: __ min

Ok

B-2
Appendix B

Parameter Review Menu

Collect: Start to End
Ok Add
or
Collect: __ to __
Ok Add Del

Select an edit path:

Time Drop Peak Manl

From this final display, you can proceed into setup

for operation in the mode of your choice.

B-3
Technical Menu C

Access the Technical menu by pressing EDIT, then 2. Following is an explanation of each
parameter in this menu.

C-1
 Appendix C
Technical Menu

GSIOC unit ID = 6
Next Quit

The unit ID identifies the 203B to a master device

(or a computer) when the two are connected via
the Gilson Serial Input/Output Channel (GSIOC).
As a default, the unit ID is set to 6. There is no need
to change this number unless it conflicts with
another unit’s ID code. For example, if you had
two 203Bs connected along the GSIOC, assign one
of them a different unit ID number.

Each ID number must identify a unique device. For

communication to occur, the 203B and the
computer must “agree” on the same unit ID
number. Use the numeric keypad to change the
unit ID.

GSIOC baud rate [ ]

Next Quit

If the fraction collector is connected to a master

device, use this parameter to set the baud rate for
data transmission between the two devices.

For most Gilson systems, the rate of transmission is

clocked outside of the device. Therefore, choose
external baud by pressing 0 on the keypad. For
example, if you connected the 203B to a Gilson
506C System Interface, make sure external baud is
selected.

When the master device does not provide a clock,

change the baud rate to the value the master device
is currently using. Press 3 for 300, 6 for 600, 12 for
1200, 24 for 2400, 48 for 4800, 96 for 9600, and 192
for 19200.

C-2
Appendix C

Technical Menu
Out 2 on [1 of seven choices]
Next No Quit

During a run, you can have the fraction collector

activate another device by connecting the
programmable output 2 (9-10 pair) to the other
device. To select the time point at which the fraction
collector activates the other device, repeatedly press
No until the screen displays the choice you want.
Then press END or Next to select that choice. See
Wiring to Other Equipment in Section 2 for more
information.

Collect positive peaks

Next Both Quit
or
Collect both peak types
Next Pos Quit

Press the Both soft key if you want the fraction

collector to collect both positive and negative peaks
during a run. Note that when the fraction collector
is set to look for negative peaks, it is less able to
accurately interpret difficult chromatograms. Press
Pos if you want only positive peaks collected.
Appendix H describes how the fraction collector
locates peaks.

Single collection head

Next Multi Quit
or
Multiple collect heads
Next Single Quit

If you’re collecting effluent from one column, set

the Single collection head option. If you want to
collect effluent from several columns at the same
time, make sure the Multiple collect head option is
selected. See Appendix G for more information
about multiple collection heads.

C-3
 Appendix C
Technical Menu

Tubes per cycle = 0

Next Quit

The Tubes per cycle parameter enables you to divide

the rack into sections with a set number of tubes
per cycle. When collection begins, each cycle will be
a full collection cycle, start to end. See Cycle
Collection in Section 3 for details on how to set
this option.

Collect peaks by slope.

Next Level Quit
or
Collect peaks by level.
Next Slope Quit

The Collect peaks by parameter identifies the

procedure that the fraction collector will use to
collect peaks while in peak mode. (See Section 3 for
information on how to set parameters in peak
mode.)

If you select the slope option, the fraction collector

collects peaks using a peak detection algorithm. To
distinguish peaks from noise, the algorithm uses the
height of the smallest peak of interest and the
width of an average peak, measured at half height.

If you select the level option, the fraction collector

uses a defined millivolt (mV) value along with the
width of an average peak, measured at half height,
for peak collection. Peaks above the specified mV
level are collected while peaks below the level are
directed to waste. Peak width is used to filter noise,
to determine the width of a safety window so the
software does not exit a peak prematurely, and to
identify when the software can exit a peak.

See Appendix H for more details on peak detection

by slope or mV level.

C-4
Service Menu D

This list of parameters is available to help service personnel confirm satisfactory

instrument performance or to aid in troubleshooting.

Access the Service menu by pressing EDIT, then 3. For an explanation of a parameter in
this menu, press the HELP key when the parameter is on the display.

D-1
 Appendix D
Service Menu

The parameters in this menu are:

A/D slope: __
Next Quit

Detector A/D: µV
Next Zero Quit

Serial port = __ h
Next Quit

Begin XY test run?

Next Test Quit

Erase all options?

Next Erase Quit

Align collection head?

Next Align Quit

Software Version 203Bv__.__

Next Quit

D-2
Dispense Head End-Fitting
Installation E

With time and use, the tip of the inlet tubing and the end fitting may become worn or
damaged.

To ensure regular drop formation, you may have to reposition or replace the Omnifit™-type
fitting or recut the end of the tubing.

E-1
 Appendix E
Dispense Head End-Fitting Installation

Replacement
To replace the end fitting, use the following
instructions:

Pull the Omnifit™ gripper off the tube. Cut the

tubing at a shallow angle with a razor blade, as
shown below.

Replace the gripper so it rests about 3 cm from the

tip. Use a needle nose pliers to pull the tubing
through the gripper.

Cut off the angled portion of tubing so it looks like

this:

The dispense head of the fraction collector contains

a gauge to indicate the distance the tubing should
extend beyond the gripper. Place the tubing
assembly into the groove on the dispense head, as
shown below.

With the gripper resting against the notch, cut the

tubing off at the cut point. Now attach the tubing
assembly to the dispense head.

E-2
Appendix E

Dispense Head End-Fitting Installation

Repositioning
If you only need to reposition the gripper or recut
the end, slide the gripper back on the tube and use
the tubing gauge to set the proper length.

E-3
GSIOC Interface F

The Gilson Serial Input/Output Channel (GSIOC) is an asynchronous serial

communications interface that enhances the power of your Gilson equipment.

It incorporates an EIA RS-485 interface and allows 32 slave devices to be controlled from
a single master in a multi-drop configuration.

A device is identified by a unique number which must be known to the device and to the
software. The default ID code of the FC 203B Fraction Collector is 6. You can set it to any
number from 0 to 63, but remember to use the same value in the software when issuing
commands to the fraction collector. Also make sure that no other unit in the system has
the same ID number.

From the software, you:

• specify the device that you want to control

• issue commands that set operating parameters, control operation, or request
information from the device

Note: For more information on the GSIOC interface and GSIOC commands, refer to the
Technical Manual for the FC 203B Fraction Collector.

To control the fraction collector via the GSIOC interface, you will need the following
equipment:

• A computer
• Gilson 506C System Interface or 605 RS-232 Adapter
• Gilson 712 HPLC Control Software, UniPoint™ System Software, or 706 Device
Driver Software and your program

F-1
 Appendix F
GSIOC Interface

GSIOC Commands
There are two kinds of commands that you can
issue from your master unit to the fraction
collector.

• Immediate commands request status

information from the fraction collector. These
one character, uppercase or symbol commands
are executed immediately, temporarily
interrupting other GSIOC commands in
progress.

• Buffered commands send instructions to the

fraction collector. These commands are
executed one at a time.

F-2
Appendix F

GSIOC Interface
Immediate Commands
Command Description

* Reads the contact status of the fraction collector inputs. Returns “abcd”
where:

a = C if input A is shorted (closed)

D if input A is open

b = C if input B is shorted (closed)

D if input B is open

c and d always = D in the FC 203B

? Reads contact status of the fraction collector outputs. Returns “abcdef”

where:

a = C if output 1 is closed
D if output 1 is open

b = C if output 2 is closed
D if output 2 is open

cdef always = D in the FC 203B

$ Resets the fraction collector. It is equivalent to restarting the unit.

% This command reads the current version. Returns “203Bvx.x” where:

x.x = currently installed software version

0 Reads FIFO 0. Format is defined by data transfer protocol.

9 Reads event FIFO. Returns “Xtttttt” where:

X = @ if contact 1 open, contact 2 open

A if contact 1 closed, contact 2 open
B if contact 1 open, contact 2 closed
C if contact 1 closed, contact 2 closed
| if no event is available in queue

tttttt = the time (in 10 ms units) since the last buffered 9 command or
000000 if the queue is empty

F-3
 Appendix F
GSIOC Interface

K Reads pressed keys. Returns “k” where:

k = ASCII code for key or null (ASCII 0)

R Reads both lines of the display. Returns “<line number 1> <line number 2> a”
where:

<line number 1> = the upper 24 characters

<line number 2> = the lower 24 characters

a = + if drain is on
- if drain is off

r Reads the top line of the display. Returns “<line number 1>” where:

<line number 1> = the upper 24 characters

T Reads current tube number. Returns “x.xx” where:

xxx = the current tube

000 indicates that the head is not over a defined tube.

V Reads raw analog input voltage. Returns “.xxxxxxV”.

Resolution is 1 microvolt.

X Reads X-axis position. Returns “axxxx” where:

a = M if moving
S if stationary

xxxx is in 0.1 mm units

Y Reads the Y-axis position. Returns “axxxx” where:

a = M if moving
S if stationary

xxxx is in 0.1 mm units

F-4
Appendix F

GSIOC Interface
Buffered Commands
Command Description

C12 Closes contact output

C1 closes output 1
C2 closes output 2
C12 closes both outputs

D12 Opens contact output

D1 opens output 1
D2 opens output 2
D12 opens both outputs

Gddd Causes the fraction collector to beep for a specified period of time:

ddd specifies beep duration (0 to 100); units are 0.1 seconds (for example,
100 = 10 seconds).

K<string> Simulates front panel key strokes via GSIOC. You can enter up to a 40-
character string. Keystrokes are simulated according to the key assignments
shown below. For example, if you wanted to start a run, you would issue
the command KS.

Ls Locks or unlocks the front panel:

s = 0 for lock front panel

1 for unlock front panel

F-5
 Appendix F
GSIOC Interface

Mxy Relaxes motors.

Mx relaxes X motor.
My relaxes Y motor.
Mxy relaxes both motors.

Warning: After you use this command, reset the fraction collector using the
immediate $ command before you issue a command that moves the head to
a new position.

Px Enables remote control of peak collection. For x, you can enter:

“p” to indicate a peak

“b” to indicate a baseline
“m” to show transition between merged peaks
“?” to have the fraction collector resume peak collection

Txxx Moves head to the specified tube. The xxx parameter indicates the number
of the tube.

Vx Flips 3-way (diverter) valve:

x = 1 for divert to drain

0 for don’t divert to drain

Wx<string> Writes text to the upper or lower line of the display:

x = 1 for the upper line

2 for the lower line

<string> can be up to 24 characters

Xxxxx Sets the X-axis position indicated by xxxx. Units are 0.1 mm towards the
right.

Yxxxx Sets the Y-axis position indicated by xxxx. Units are 0.1 mm towards the
front.

0xxxx Sets FIFO 0 sampling rate and clears FIFO. The parameter xxxx is the
sampling rate in units of 0.01 Hz.

9 Clears the contact closure event FIFO and resets the contact event timer.

F-6
Multiple Column Option G

The FC 203B Fraction Collector’s multiple column option allows you to collect effluent
from several columns at the same time.

You can use the multiple column option with any Gilson rack, in any operating mode. To
easily synchronize collection from several columns, Gilson recommends that you
choose Time mode as the operating mode.

Before you use this option, use the following instructions to:

• prepare your columns

• select the multiple column option
• install and adjust the multiple column adapter
• install a rack

G-1
 Appendix G
Multiple Column Option

Set-up
Prepare as many separation columns as you will
need. Attach the column outlet tubings to the
columns.

Note: Remember to make the outlet tubings long

enough to move with the multiple column adapter
during the run!

Column Option Display

To access the multiple column option from the
Technical menu, press EDIT 2, then Next until you
see:

Single collection head

Next Multi Quit

or

Multiple collect heads

Next Single Quit

• If the first line shows Single collection head, press

Multi to tell the fraction collector that you’ll run
multiple columns. Press Quit to return to the
operating mode you selected.

• If the first line shows Multiple collect heads, the

fraction collector is already set to use multiple
columns. Press Quit to return to the operating
mode you selected.

When you choose the multiple column option, the

motor that controls front to back movement of the
dispense head is relaxed. During collection, the
dispense arm will move only from left to right
(when viewed from the front of the fraction
collector).

G-2
Appendix G

Multiple Column Option

Install Column Adapter
Push the drop detector head to the rear of the
dispense arm.

With the nylon thumbscrew facing the left hand

side of the fraction collector, slide the multiple
column adapter onto the dispense arm.

• If you will collect in manual, time, or peak + time

mode, push the multiple column adapter as far
back as it will go. Tighten the thumbscrew.

• In drop mode and peak + drop mode only, you

can key tube advance to the number of drops
passing the drop detector. However, you will
not be able to collect fractions in the row of
tubes adjacent to the drop detector head.

If you choose to collect in a drop mode, position

the drop detector over the tube closest to the rear
of the fraction collector. Then push the multiple
column adapter back until it touches the drop
detector head. Tighten the thumbscrew.

G-3
 Appendix G
Multiple Column Option

Connect Outlet Tubing

Connect each column outlet to an adjustable tubing
holder on the multiple column adapter.

Place an Upchurch flangeless nut and ferrule onto the

end of each outlet tubing. Your accessory package
contains nuts and ferrules for 1/16" and 1/8" tubing.
Be sure the cone of the ferrule faces the nut.

Loosely screw the fitting into the top of the tubing

holder. Place the Allen wrench into the set screw
on the tubing holder.

Push the tubing through the fitting until it touches

the Allen wrench. Then fingertighten the 1/4-28
fitting.

Note: If you will collect in a drop mode, as

described above, you must attach one column
outlet to the drop detector head. To make this
connection, follow the Plumbing Setup
instructions in Section 2.

G-4
Appendix G

Multiple Column Option

Install Rack
Select and install a rack, considering these points:

• Fractions eluting from each separation column

are collected in a single row from left to right
across the length of each rack. You must
allocate one row for each chromatographic
column.

• Each row must contain enough vessels to collect

the desired number of fractions.

Example: A Code 1 rack holds 80 tubes

arranged in a 5 x 16 array. With this rack
installed, you could collect up to 16 fractions
from as many as five columns.

• If a microplate holder and microplate are

installed, indicate rack code “17” to collect into
the microplate.

Align Outlet Tubings

Carefully align the outlet tubing from each column
so effluent will fall into a single row of tubes in the
rack.

Using the Allen wrench, tighten each adjustable

tubing holder when you are certain that it is
properly aligned.

Note: The outlet tubing will not be directly over the

tube until the run starts. You should ensure that
the drops will fall into the tube by looking at the
tubing holders from the right-hand side of the
fraction collector.

G-5
 Appendix G
Multiple Column Option

Begin the Run

Begin the collection by pressing START. Notice that
instead of showing the current tube number, the
display shows the number of the fraction being
collected.

End the Run

After the final fraction has been collected, a short
alarm will sound. At that time, you should follow
this method to prevent extraneous material from
dripping into the tubes:

• stop the flow from the columns! You must do

this first.

• press END to finish the run. The dispense head

will remain at the right-hand side of the rack
until you press END.

• remove the rack of tubes.

Note: If you are using the programmable output

(Out2) to control another device, be aware of the
following change that is in effect when collecting
from several columns.

If the output was scheduled to turn a device on or

off at the end of a run, it will now do so after the
last fraction has been collected.

Return to Single Column

Collection
When you want to return to single column
collection, remove the multiple column adapter.

Then access the column option from the Technical

menu (EDIT 2) and choose Single. The display will
remind you that the dispense arm is being reset.

You can then proceed with single column

collection.

G-6
Peak Detection Using
Slope or Millivolt Level H

In the Technical menu (EDIT 2), you indicate if the fraction collector should collect peaks
using slope or millivolt level. (Collection by slope is the default.) This appendix describes
the peak detection algorithm that the fraction collector uses to collect peaks if the slope
option is selected. It also looks at what peaks are collected if you choose the level option.

H-1
 Appendix H
Peak Detection Using Slope or Millivolt Level

Detection Using Slope (Peak

Detection Algorithm)
To distinguish peaks from baseline noise, you tell
the fraction collector the following information
when setting parameters in the peak mode:

• height of the smallest peak of interest

• width of an average peak, measured at half
height

You can get the above information by running a

preliminary sample.

H-2
Appendix H

Peak Detection Using Slope or Millivolt Level

Master Slope
When you enter values for minimum peak height
and for average peak width, the fraction collector
determines the master slope of the smallest peak
that it will detect.

Master slope (S0) =(k0) Peak height

Peak width

As you increase the peak width setting and/or

decrease the peak height setting, the master slope
decreases and the peak-detection sensitivity
increases.

H-3
 Appendix H
Peak Detection Using Slope or Millivolt Level

Peak Phases
A positive peak is composed of three phases (refer
to diagram below):

• a rising slope
• an apex
• a falling slope

The fraction collector uses a phase-specific slope

detection method to determine the presence of a
peak. Critical slopes at four points define the three
peak phases and the return to baseline.

Those critical slopes are related to the master slope, S0:

rising slope (S1) = k1S0

apex slope (S2) = k2S0
falling slope (S3) = k3S0
baseline slope (S4) = k4S0

H-4
Appendix H

Peak Detection Using Slope or Millivolt Level

Maximum Peak Width
The maximum valid width of a peak is also
determined from the master slope parameters (peak
height and width):

Maximum width = k 5S 0

(k0 through k5 are programmed constants)

When the maximum peak width is exceeded, the

fraction collector decides that a baseline shift has
occurred and it resets the baseline appropriately.

Special Cases
If you desire, you can collect negative peaks. The
three negative-peak phases are falling front, bottom,
rising end. In general, when the fraction collector is
set to look for negative peaks, it is less able to
accurately interpret difficult chromatograms. The
default setting is for collection of positive peaks only.
To change that setting, see Appendix C.

The fraction collector can identify merged peaks,

recognizing that the falling slope phase of one peak
is directly adjacent to the rising slope phase of the
next. Positive merged peaks and negative merged
peaks can be identified.

H-5
 Appendix H
Peak Detection Using Slope or Millivolt Level

Detection Using Millivolt Level

To collect peaks using a millivolt (mV) level, you tell
the fraction collector the following when setting
parameters in the peak mode:

• width of an average peak, measured at half

height
• mV value above which all peaks will be
collected

You can get the above information by running a

preliminary sample.

The fraction collector uses the mV value along with

the peak width for peak collection. Peaks above the
specified mV level are collected while peaks below
the level are directed to waste. Peak width is used
to filter noise, to determine the width of a safety
window so the software does not exit a peak
prematurely, and to identify when the software
can exit a peak.

As a recommendation, set the mV level at 2 times

the expected baseline fluctuation. For example, if
the baseline stays between 0 and 1 mV during
collection, set the level at 2 mV.

The shaded peak areas and event marks in the

following chromatogram plots show how setting
the level at different mV values affects the amount
of effluent collected for peaks.

H-6
Appendix H

Peak Detection Using Slope or Millivolt Level

Level set to 1.0 mV

time (minutes)

Level set to 0.2 mV

time (minutes)

H-7
 Appendix H
Peak Detection Using Slope or Millivolt Level

Level set to 4.0 mV

time (minutes)

H-8
Autopreparative Collection Controlled
by 712 HPLC Control Software I

In a Gilson autopreparative HPLC system, one high-pressure pump repetitively injects

sample. The autopreparative system can be an isocratic or a gradient system and will
include a fraction collector, such as the 203B.

To coordinate system components, you’ll use Gilson 712 HPLC Control Software or
UniPoint™ System Software. Within the software, you’ll set parameters that control the
devices via the Gilson Serial Input/Output Channel (GSIOC) or by sending contact
signals.

This appendix describes how to set up system components for the autopreparative
collection of fractions using the 712 HPLC Control Software. You’ll find information on
making rear panel connections, setting parameters on the fraction collector, and setting
parameters in the 712 HPLC Control Software. In the examples, the injection pump has a
10 SC head, and the injection volume is 5 mL at a flow rate of 5 mL/min. Also four
samples will be injected and eight fractions will be collected from each sample.

To learn about controlling an autopreparative system using UniPoint, please request a

copy of the Large-Volume Preparative System Control Using a 30x Pump for Repetitive
Injection Technical Note (part number LT21375) from your Gilson representative.

I-1
 Appendix I
Autopreparative Collection Controlled by 712 HPLC Control Software

Connecting System Components

This section describes the electrical connections you
need to make between the fraction collector, 506C
System Interface, and a detector.

GSIOC Connections
To control the pumps and send GSIOC commands
to other system components, use GSIOC cables to
connect system components to the 506C System
Interface. Then connect the RS-232 cable to the
serial ports of the 506C and the computer.

If necessary, refer to the System Installation manual

that came with the control software.

Contact Connections
Use 2-conductor cable to make contact connections
between the fraction collector and the 506C.

To remotely start the fraction collector, send an

output signal to the fraction collector from the
computer via the 506C. Connect the fraction
collector Input A to the 506C Output 1. Be sure to
match ground connections. Page I-13 describes
how to set up the event to send the output signal
from the computer.

To mark tube advance directly on the data chart,

connect the fraction collector event mark output
(Output 1, 7-8 pair) to Input A of the 506C. Be sure
to match ground connections.

Note: If you’re collecting in peak mode and also

want an event mark at peak start, see page 2-17.

I-2
Appendix I

Autopreparative Collection Controlled by 712 HPLC Control Software

Detector Connections
In addition, if collecting in peak mode, connect the
detector to the fraction collector’s DET input. (If
you’re using a Gilson detector, you’ll need a
Pomona cable adapter, part number 6374022611.)
Connect the fraction collector’s DET pin 1 to the
detector’s “+” output and the fraction collector’s
DET pin 2 to the detector’s “–” (or GND) output.

Note: If you’re using a non-Gilson detector, you may

need to connect the DET pin 2 to the metal screw in
the lower left or right corner of the fraction collector
to reduce ground noise. Refer to page 2-14.

Connection for a non-Gilson detector Connection for a Gilson detector

I-3
 Autopreparative Collection Controlled by 712 HPLC Control Software

I-4
Appendix

Contact and Detector Connections for 203B and 506C

I
Appendix I

Autopreparative Collection Controlled by 712 HPLC Control Software

Programming the 203B
The fraction collector enables you to collect into the
same set of tubes or into a different set of tubes in
each cycle.

The following examples show how to set up

parameters for a run using the time mode. One
example collects each cycle into the same set of
tubes while the other collects into a different set of
tubes.

Example 1
Collection of Each Cycle Into Same
Set of Tubes
When setting collection parameters on the fraction
collector, you can indicate that you want to collect
each cycle into the same set of tubes.

For example, if you have a Code 0 (zero) rack

installed, each sample will be collected into the
same set of tubes per collection cycle. Collection
will be made into the following tubes for each
cycle.

Cycle Tubes
1 1–80
2 1–80
3 1–80
4 1–80
etc. etc.

I-5
 Appendix I
Autopreparative Collection Controlled by 712 HPLC Control Software

Review or change rack

When the fraction collector “wakes up”, it

remembers and displays the last set of instructions it
was given, for example:

Time mode 1.00 min/tube

List Rack 0 Start

To display a description of the current rack, press

the Rack soft key. You’ll see:

Rack code __ selected

[description of rack] Ok

If a different rack is installed, enter the number

code for the rack. The code for most racks is
imprinted on the rack.

Press Ok.

Check tubes per cycle parameter

To indicate that you want to collect into the same

set of tubes for each cycle, check that the Tubes per
cycle parameter has been set to 0 (zero).

Press EDIT, then 2 to access the Technical menu.

Now press the Next soft key until you see:

Tubes per cycle = __

Next Quit

If the tubes per cycle is not set to 0 (zero), use the

numeric keypad to change it.

I-6
Appendix I

Autopreparative Collection Controlled by 712 HPLC Control Software

Select time mode

Press the EDIT key. You’ll see the mode selection

display:

Select an edit path:

Time Drop Peak Manl

Press the Time soft key.

Time per tube selection

You’ll see the time selection display:

Time mode __ min/tube

Ok

Press the NO key to clear the display. Then enter

the desired time per tube from 0.01 to 99.99
minutes. For this example, indicate 0.05 minute per
tube.

Press Ok.

Collection window option

You’ll see the collection window display:

Collect: __to __
Ok Add Del

Press Ok until the ready-to-run display appears.

You’re done setting parameters.

Time mode 0.05 min/tube

List Rack 0 Start

I-7
 Appendix I
Autopreparative Collection Controlled by 712 HPLC Control Software

Example 2
Collection of Each Cycle Into a
Different Set of Tubes
When setting collection parameters, you can tell the
fraction collector to collect each cycle into a
different set of tubes.

Review or change rack

When the fraction collector “wakes up”, it

remembers and displays the last set of instructions it
was given, for example:

Time mode 1.00 min/tube

List Rack 0 Start

To display a description of the current rack, press

the Rack soft key.

You’ll see:

Rack code __ selected

[description of rack] Ok

If a different rack is installed, enter the number

code for the rack. The code for most racks is
imprinted on the rack.

Press Ok.

Set tubes per cycle

To indicate that you want to collect into a different

set of tubes for each cycle, access the Technical
menu.

Press EDIT, then 2. Now press the Next soft key

until you see:

Tubes per cycle = 0

Next Quit

I-8
Appendix I

Autopreparative Collection Controlled by 712 HPLC Control Software

The Tubes per cycle parameter enables you to divide
the rack into sections with a set number of tubes
per cycle.

To collect each sample into a new set of tubes, use

the numeric keypad to indicate a number greater
than 0 (zero). Using the tubes per cycle and the
total tube capacity for the rack, the number of
cycles is calculated.

Note that if the number of tubes can’t be divided

into the tube capacity evenly, the number of cycles
is the whole-number quotient; the remaining tubes
are not used.

If you set 20 as the number of tubes per cycle for a

code 0 (zero) rack, the number of cycles would be
4. Collection will be made into the following tubes
for each cycle.

Cycle Tubes
1 1–20
2 21–40
3 41–60
4 61–80

Note: The tubes per cycle option cannot be used in

conjunction with the 203B’s multiple collection
head option. If you try to set the tubes per cycle
option when the multiple collection head option is
active, you will be prompted to change the Multiple
collect heads parameter to single.

Select time mode

Press the EDIT key. You’ll see the mode selection

display:

Select an edit path:

Time Drop Peak Manl

Press Time.

I-9
 Appendix I
Autopreparative Collection Controlled by 712 HPLC Control Software

Time per tube selection

You’ll see the time selection display:

Time mode __ min/tube

Ok

Press the NO key to clear the display. Then enter the

desired time per tube from 0.01 to 99.99 minutes. For
this example, indicate 0.05 minute per tube.

Press Ok.

Collection window option

You’ll see the collection window display:

Collect: __ to __
Ok Add Del

Press Ok until the ready-to-run display appears.

You’re done setting parameters.

Time mode 0.05 min/tube

List Rack 0 Cycle 1

I-10
Appendix I

Autopreparative Collection Controlled by 712 HPLC Control Software

Coordinating with 712 HPLC
Control Software
To control the components of the autopreparative
HPLC system, use the Gilson 712 HPLC Control
Software. Within the software, indicate the control
and analysis conditions for the run. For detailed
information on creating a method, consult your 712
HPLC Control Software User’s Guide.

An example of a 712 method is listed on pages I-16

to I-17. You should also be aware of the following
when setting parameters for the method.

Configuration
Do not include the injection pump in the Hardware
Configuration box (Edit menu/Hardware Config.
command). The pump information in that box is
for mobile phase delivery pumps only because the
software limits flow to the maximum flow rate of
the smallest pump head size specified. Typically,
the pumps in the system are arranged as shown
below.

Pump Unit ID Function

A 1 Elution pump
B 2 Elution pump
C 4 Injection pump (not
included in Hardware
Configuration box)

Number of Loops
The number of Loop Passes set in the Run
Conditions box (Edit menu/Run Conditions
command) indicates the number of samples to be
injected. This number is the same as the number of
fraction collector cycles.

I-11
 Appendix I
Autopreparative Collection Controlled by 712 HPLC Control Software

Mobile Phase
When setting up the method, indicate a schedule of
timed flow events using the Mobile Phase box (Edit
menu/Mobile Phase command). That schedule
identifies the flow rate and percent composition
that each elution pump contributes to the flow at
various time points during the run. At the time of
injection, set the flow rate from the elution pumps
at 0.00 mL/min.

Following is a schedule for a gradient run. During

injection (5.00 to 5.50 min.), Pumps A and B are
not pumping mobile phase.

Time (min.) Flow (mL/min.) %B

0.00 30.000 5.0
4.99 30.000 5.0
5.00 0.000
5.50 0.000
5.57 30.000 5.0

At 4.99 min. into the run, the flow rate is 30.0 mL/
min. with 5% coming from Pump B and 95% coming
from Pump A. %A is calculated as 100% – %B.

Between 5.00 and 5.50 min., the elution pumps are

not pumping. This is the time of injection.

At 5.57 min., Pumps A and B are once again

pumping a total of 30.0 mL/min. with the percent
composition remaining the same until the end of
the run.

Note: You’ll set up a GSIOC event that tells the

injection pump when and how much sample to
pump. You’ll also set up a GSIOC event that checks
the status of the injection pump and makes sure
that 0 (zero) injection cycles remain. That is why
there is a gap between time points 5.50 and 5.57.

See GSIOC Events, on the next page, for more

information on setting up the required GSIOC
events.

I-12
Appendix I

Autopreparative Collection Controlled by 712 HPLC Control Software

Contact Events
In the Contact Event Table (Edit menu/Contact
Events command), set up an event that sends a
pulse signal to the fraction collector. Send the
signal after injection is completed. This starts the
collection cycle set up on the fraction collector.

Time Contact Action Description

6.00 1 Pulse Sends a pulse
signal via
output 1 to the
fraction
collector.

Also, label Input A as “FC 203B event mark” if you

have set up this connection as described on page I-2.

GSIOC Events
In the GSIOC Event Table (Edit menu/GSIOC
Events command), set up events to:

• Lock out the front panel of the injection pump

and enable the remote control function using
the buffered L (Lock) command.

• Set the remote mode and lock the keypad for

the fraction collector using the buffered SR (Set
Remote) command.

• Dispense the sample using the injection pump.

To do this, you’ll use three buffered commands:

- The D command sets the dispense mode.

- The Eabcde command sets the dispense

parameters.

To calculate the 3-digit number corresponding to

abc, use this formula: abc = (dispense volume ÷
head size) x 1000. For example: abc = (5 mL ÷ 10) x
1000 = 500.

I-13
 Appendix I
Autopreparative Collection Controlled by 712 HPLC Control Software

The parameter d is the refill speed of the piston and

e is the dispense rate. To determine these, use the
following table. Within the table, first locate the
flow rate. Then find the corresponding refill speed
and dispense rate. For example, if the flow rate is
5.0, the refill speed (d) is 4 and the dispense rate (e)
is 3. See table below.

Note: The following table is for a 10 SC head. If

you’re using a 5 SC head, divide the flow rate you
want to obtain by 2; then locate that flow rate in
the table. If you’re using a 25 SC head, multiply the
flow rate you want to achieve by 2.5; then locate
that flow rate in the table.

- The buffered Bn command indicates

the number of times (n) to repeat the
dispense cycle. If n is omitted, the
default is 1 dispense cycle.

I-14
Appendix I

Autopreparative Collection Controlled by 712 HPLC Control Software

• Request the status of the injection pump using
the immediate P command. When the pump
responds that 0 (zero) cycles remain, the
computer continues the programmed method.

• (Optional) Read the current tube number into

the 712 analysis report using the immediate T
command. This will identify the tube at which
the fraction collector started collecting the
sample.

Refer to the following table to set up the GSIOC

events for the above commands.

Time Unit Command Command Description

ID Type

0.01 4 B L Lock out front panel of injection pump.

0.10 6 B SR Set the fraction collector in remote

mode.

5.00 4 B DE50043B1 Is the injection command for the

injection pump:
D Sets the dispense mode.
E50043 Sets the dispense parameters
(see description on previous
page).
B1 Indicates the number of times
to repeat the dispense cycle.

5.50 4 I P Request status of injection pump.

When the pump responds that 0 (zero)
cycles remain, the computer continues
the programmed method.

6.02 6 I T># Read current tube number into 712

analysis report. For #, enter number
from 1 to 9 to indicate report line on
which tube number will be listed. (Note
that line 0 is reserved for sample ID.)

I-15
 Appendix I
Autopreparative Collection Controlled by 712 HPLC Control Software

Method Listing
Refer to the following method listing when setting parameters for an autopreparative
run.

Listing of Method: \GILSON\712_AUTO.USR\AUTOPREP.MTH

-Control Parameters-
Run Time: 30.00 min
Loop Passes: 4
1.00 min Loop Begin
30.00 min Loop End
Link Method : STOP

-Mobile Phase Events-

0.00 min Flow = 30.000 %B = 5.0
4.99 min Flow = 30.000 %B = 5.0
5.00 min Flow = 0.000
5.50 min Flow = 0.000
5.57 min Flow = 30.000 %B = 5.0

-Contact Events-
6.00 min Pulse 1 Start frac. coll.

-GSIOC Events-
0.01 min Buf_Cmd to unit 4: L 'Lock injection pump front panel
0.10 min Buf_Cmd to unit 6: SR 'Set frac. collector to remote mode
5.00 min Buf_Cmd to unit 4: DE50043B1 'Set dispense speed and volume
5.50 min Imm_Cmd to unit 4: P 'Read cycle#; if NB=0, stop injection
6.02 min Imm_Cmd to unit 6: T>1 'Read tube number into report

-Analysis Parameters-
Integration Start: 5.51 min
Integration Time: 24.49 min
Peak Width: 0.20 min
Peak Sensitivity: 2.0 %

Analysis Channel: A
Save the data
mV Full Scale: 10
% Offset: 10
Chart speed is 10 mm/min
Do not print the strip chart

I-16
Appendix I

Autopreparative Collection Controlled by 712 HPLC Control Software

-Analysis Report-
Area percent report
Save the report
Report unnamed peaks

-Scaled Plot-
Do not print scaled plot
Plot Analysis Channel (A)

-Rubber Stamp-

-Analysis Peak Table-

none

-Analysis Events-
none

-Method Setup-
Number of Pumps: 2
Contact Unit ID: 63
Data Unit ID: 63

ID Headsize Refill Comp.

Pump A 1 50 250 46
Pump B 1 50 250 99

-Chronological List of Events-

0.00 min Flow =30.000 %B = 5.0
0.01 min Buf_Cmd to unit 4: L 'Lock injection pump front panel
0.10 min Buf_Cmd to unit 6: SR 'Set frac. collector to remote mode
1.00 min Loop Begin
4.99 min Flow = 30.000 %B = 5.0
5.00 min Buf_Cmd to unit 4: DE50043B1 'Set dispense speed and volume
Flow = 0.000
5.50 min Imm_Cmd to unit 4: P 'Read cycle#; if NB=0, stop injection
Flow = 0.000
5.51 min Integration Start
5.57 min Flow = 30.000 %B = 5.0
6.00 min Pulse 1 Start frac. coll.
6.02 min Imm_Cmd to unit 6: T>1 'Read tube number into report
30.00 min Loop End

I-17
Autopreparative Collection
Controlled by 305 Pump J

In a Gilson autopreparative HPLC system, one high-pressure pump repetitively injects

sample. The autopreparative system can be an isocratic or a gradient system and will
include a fraction collector, such as the 203B.

To coordinate system components, you’ll use a Gilson 305 Pump. Using the 305’s
software, you’ll set mobile phase composition, identify injection pump parameters, and
coordinate fraction collection on the 203B.

This appendix describes how to set up system components for the autopreparative
collection of fractions. You’ll find information on making rear panel connections, setting
parameters on the fraction collector, and setting parameters on the 305.

J-1
 Appendix J
Autopreparative Collection Controlled by 305 Pump

Connecting System Components

This section describes the electrical connections you
need to make between the 203B, 305, and detector.

GSIOC Connections
To control the injection pump, use a GSIOC cable
to connect the controlling 305, elution pump(s),
and injection pump. If necessary, refer to the 305
User’s Guide for more information on making
GSIOC connections.

Contact Connections
Use 2-conductor cable to make contact connections.

To remotely start the fraction collector, send an

output signal from the 305 Pump to the fraction
collector. Connect the fraction collector Input A to
the 305 Output 1 (10-11 pair). Be sure to match
ground connections.

To mark tube advance directly on the data chart,

connect the fraction collector event mark output
(Output 1, 7-8 pair) to the appropriate input pair of
the data unit (e.g., chart recorder, integrator, or
non-Gilson software). Be sure to match ground
connections.

If your data unit cannot accept input from an event

mark output, you may connect the event mark
output pair to the appropriate input pins of the
detector. When set up this way, the data unit may
interpret event marks as negative peaks on the
detector trace.

J-2
Appendix J

Autopreparative Collection Controlled by 305 Pump

Detector Connections
In addition, if collecting in peak mode, connect the
detector to the fraction collector’s DET input. (If
you’re using a Gilson detector, you’ll need a Pomona
cable adapter, part number 6374022611.) Connect
the fraction collector’s DET pin 1 to the detector’s
“+” output and the fraction collector’s DET pin 2 to
the detector’s “–” (or GND) output.

Note: If you’re using a non-Gilson detector, you

may need to connect the DET pin 2 to the metal
screw in the lower left or right corner of the
fraction collector to reduce ground noise. Refer to
page 2-14.

Connection for a non-Gilson detector Connection for a Gilson detector

J-3
 Appendix J
Autopreparative Collection Controlled by 305 Pump

Contact and Detector Connections for 203B and 305

J-4
Appendix J

Autopreparative Collection Controlled by 305 Pump

Programming the 203B
The 203B allows you to collect into the same set of
tubes or into a different set of tubes in each cycle.

The following examples show how to set up

parameters for a run using the time mode. One
example collects each cycle into the same set of
tubes while the other collects into a different set of
tubes.

Example 1
Collection of Each Cycle Into Same
Set of Tubes
When setting collection parameters on the fraction
collector, you can indicate that you want to collect
each cycle into the same set of tubes.

For example, if you have a Code 0 (zero) rack

installed, each sample will be collected into the
same set of tubes per collection cycle. Collection
will be made into the following tubes for each
cycle.

Cycle Tubes
1 1–80
2 1–80
3 1–80
4 1–80
etc. etc.

J-5
 Appendix J
Autopreparative Collection Controlled by 305 Pump

Review or change rack

When the fraction collector “wakes up”, it

remembers and displays the last set of instructions
it was given, for example:

Time mode 1.00 min/tube

List Rack 0 Start

To display a description of the current rack, press

the Rack soft key. You’ll see:

Rack code __ selected

[description of rack] Ok

If a different rack is installed, enter the number

code for the rack. The code for most racks is
imprinted on the rack.

Press Ok.

Check tubes per cycle parameter

To indicate that you want to collect into the same

set of tubes for each cycle, check that the Tubes per
cycle parameter has been set to 0 (zero).

Press EDIT, then 2 to access the Technical menu.

Now press the Next soft key until you see:

Tubes per cycle = __

Next Quit

If the tubes per cycle is not set to 0 (zero), use the

numeric keypad to change it.

Select time mode

Press the EDIT key. You’ll see the mode selection

display:

Select an edit path:

Time Drop Peak Manl

Press the Time soft key.

J-6
Appendix J

Autopreparative Collection Controlled by 305 Pump

Time per tube selection

You’ll see the time selection display:

Time mode __ min/tube

Ok

Press the NO key to clear the display. Then enter

the desired time per tube from 0.01 to 99.99
minutes. For this example, indicate 0.05 minute per
tube.

Press Ok.

Collection window option

You’ll see the collection window display:

Collect: __to __
Ok Add Del

Press Ok until the ready-to-run display appears.

You’re done setting parameters.

Time mode 0.05 min/tube

List Rack 0 Start

J-7
 Appendix J
Autopreparative Collection Controlled by 305 Pump

Example 2
Collection of Each Cycle Into a
Different Set of Tubes
When setting collection parameters, you can tell the
fraction collector to collect each cycle into a
different set of tubes.

Review or change rack

When the fraction collector “wakes up”, it

remembers and displays the last set of instructions
it was given, for example:

Time mode 1.00 min/tube

List Rack 0 Start

To display a description of the current rack, press

the Rack soft key.

You’ll see:

Rack code __ selected

[description of rack] Ok

If a different rack is installed, enter the number

code for the rack. The code for most racks is
imprinted on the rack.

Press Ok.

J-8
Appendix J

Autopreparative Collection Controlled by 305 Pump

Set tubes per cycle

To indicate that you want to collect into a different

set of tubes for each cycle, access the Technical
menu.

Press EDIT, then 2. Now press the Next soft key

until you see:

Tubes per cycle = 0

Next Quit

The Tubes per cycle parameter enables you to divide

the rack into sections with a set number of tubes
per cycle.

To collect each sample into a new set of tubes, use

the numeric keypad to indicate a number greater
than 0 (zero). Using the tubes per cycle and the
total tube capacity for the rack, the number of
cycles is calculated.

Note that if the number of tubes can’t be divided

into the tube capacity evenly, the number of cycles
is the whole-number quotient; the remaining tubes
are not used.

If you set 20 as the number of tubes per cycle for a

Code 0 (zero) rack, the number of cycles would be
4. Collection will be made into the following tubes
for each cycle.

Cycle Tubes
1 1–20
2 21–40
3 41–60
4 61–80

Note: The tubes per cycle option cannot be used in

conjunction with the fraction collector’s multiple
collection head option. If you try to set the tubes
per cycle option when the multiple collection head
option is active, you will be prompted to change
the Multiple collect heads parameter to single.

J-9
 Appendix J
Autopreparative Collection Controlled by 305 Pump

Select time mode

Press the EDIT key. You’ll see the mode selection

display:

Select an edit path:

Time Drop Peak Manl

Press Time.

Time per tube selection

You’ll see the time selection display:

Time mode __ min/tube

Ok

Press the NO key to clear the display. Then enter

the desired time per tube from 0.01 to 99.99
minutes. For this example, indicate 0.05 minute per
tube.

Press Ok.

Collection window option

You’ll see the collection window display:

Collect: __ to __
Ok Add Del

Press Ok until the ready-to-run display appears.

You’re done setting parameters.

Time mode 0.05 min/tube

List Rack 0 Cycle 1

J-10
Appendix J

Autopreparative Collection Controlled by 305 Pump

Coordinating with 305 Pump
You’ll set up the 305 to control the mobile phase
composition, control injection pump parameters,
and coordinate fraction collection on the 203B. If
necessary, refer to the 305 User’s Guide for
information on setting parameters and creating
program files.

The pumps in the system are arranged as follows.

Pump A is the controller and must be a 305. Pump
B can be any model Gilson pump. The injection
pump must be a 305, 306, or 307 if the controlling
305 is V1.2 or higher.

Pump Unit ID Function

A 1 Elution pump
B 2 Elution pump
Inj. 4 Injection pump

Set Up Pump Hardware

To set conditions for elution pump A, which is also
the controller:

• Access the PUMP menu.

• Select pump A.
• Enter 125 ms for refill time.
• Enter compressibility of mobile phase pumped
by pump A.
• Enter 50 SC for pump head size.

To set conditions for elution pump B:

• Access the PUMP menu.

• Select pump B.
• Enter 125 ms for refill time.
• Enter compressibility of mobile phase pumped
by pump B.
• Enter 50 SC for pump head size.

J-11
 Appendix J
Autopreparative Collection Controlled by 305 Pump

To set conditions for the injection pump:

• Access the PUMP menu.

• Select pump Inj.
• Enter 125 ms for refill time.
• Enter compressibility of sample pumped by the
injection pump.
• Enter 10 SC for pump head size.

Set Up Contact
In the I/O menu, set Output #1 to the open state.

Set Up Program File

In the File menu, create a program file for the run.
Indicate the following in the file.

# of loops: number of samples to be injected

MIXT: Time %B
0 5
30 5

FLOW: Time Flowrate

0 30
30 30

INJ: Time Inj. Vol. Inj. Rate

5 5 ml 5 ml/min

OUT: Time Output State

5.1 1 Pulse

Program Sheet
Refer to the attached programming sheet when
setting up parameters for the run.

J-12
 Autopreparative Collection Controlled by 305 Pump

J-13
J
Appendix
Collecting Sharp Narrow Peaks K

With the FC 203B Fraction Collector, peak detection is optimized for LC applications that
collect peaks with a width at half height of 1 minute or longer. For HPLC applications
that collect varying sizes of sharp narrow peaks, you may need to:

• connect the DET input of the fraction collector to the 10 mV full scale output of the
detector, if the fraction collector is connected to the 100 mV full scale output.

• adjust the peak width and peak height values set in the fraction collector’s software.
Peaks with widths of approximately 0.4 minutes or less may require peak width and
height settings that are two to five times greater than the average peak width and
actual peak height. Consequently, the fraction collector may not be able to detect small
peaks (5 to 10% of full scale) and large peaks (100% of full scale) within the same
chromatogram.

The chromatograms on the next few pages show how to improve collection of sharp
narrow peaks. The shaded peak areas and event marks identify what’s being collected by
the fraction collector.

K-1
 Appendix K
Collecting Sharp Narrow Peaks

In Figure 1, peak width and height are set as

suggested in Appendix H, Peak detection
algorithm. With those settings, only half of the
large peak at 4.4 minutes is collected.

Figure 1

Next see how an increase in peak height affects

peak collection. In Figure 2, the peak height is
increased by more than five times the setting used
in Figure 1, and peak width is not changed. The
large peak is now collected, but most of the small
peaks are missed.

Figure 2
K-2
Appendix K

Finally see how an increase in peak width and

Collecting Sharp Narrow Peaks

peak height affect peak collection. In Figure 3, peak
width is increased by more than four times the
setting used in Figure 1. (Refer to the second bullet
point on page K-1.) With these new values, more of
the small peaks and all of the large peak are
collected.

Figure 3

If your fraction collector is operating in a system

controlled by Gilson UniPoint™ System Software,
the peak width and sensitivity settings in the
method can be used to determine the peaks
detected by the fraction collector. These settings are
specified in the Analysis Parameters and Analysis
Events boxes. Consult the UniPoint™ System
Software User’s Guide for more information.

K-3
Replacement Parts and Accessories L

Part number Description

174732 Multiple column adapter

170730 3-way valve kit

709910206 2-conductor interconnect wire; 6 ft.

6374022611 Pomona cable adapter

36078143 Shielded GSIOC cable, 30 in.

100731 Event cable to Gilson detectors

1707305 Small bore needle for Code 2 and Code 15 racks with 3-way
valve

17013004 Front drain trough

1703548 Inlet drop tubing assembly

1707303 Standard bore needle for 3-way valve

1307302 Inlet tubing assembly for 3-way valve, 2 ft.

1307303 Drain tubing assembly for 3-way valve, 2 ft.

F1410050 Coupling for 1/4"-28 fittings; package of 5

1704731 Polypropylene pan

170472 Tube retaining bar for Code 1 rack

170471 Rack holder assembly for Code 0, 1, 2, 4, 11, and 14 racks

L-1
 Appendix L
Replacement Parts and Accessories

17074304 Drain tubing for drain trough

638310512 10-pin terminal block connector

170350V Drop detector assembly, Valox. Standard accessory.

170350 Drop detector assembly, Delrin. Delrin has strong chemical

resistance to most organic solvents and most neutral-pH
aqueous solvents. Not suitable for use with acids, bases,
or oxidizing agents.

17235003 Replacement glass sleeves; package of 6

708038107 Power cord; 115V

7080316106 Power cord; 220V

6730054007 0.5 amp T-0.5 replacement fuse, Slo-Blo

L-2