H201-T-UNIT-BL

Temperature Control Unit

Manual

IST1254_REV01
SV 3.5.4.3048

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 Index
1 PREFACE ..................................................................................................................................................................................... 3
2 SYMBOL DESCRIPTION .......................................................................................................................................................... 4
SYMBOLS USED IN THIS MANUAL ...............................................................................................................................................................4
SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................4
3 SAFETY NOTES .......................................................................................................................................................................... 5
4 SUPPLIED EQUIPMENT .......................................................................................................................................................... 7
5 NOT SUPPLIED EQUIPMENT ................................................................................................................................................ 7
OPTIONAL EQUIPMENT ...............................................................................................................................................................................8
6 EQUIPMENT DESCRIPTION .................................................................................................................................................. 9
7 INSTALLATION ...................................................................................................................................................................... 11
H201-T-UNIT-BL. INSTALLATION GUIDE ......................................................................................................................................... 11
HOW TO CONNECT H201-T-UNIT-BL TO A BOLD LINE DIGITAL GAS CONTROLLER ............................................................. 13
8 USER INTERFACE .................................................................................................................................................................. 14
HOME PAGE ................................................................................................................................................................................................ 14
8.1.1 How to enter the set point temperature ........................................................................................................................... 14
8.1.2 Controller Status: colours led and meaning .................................................................................................................... 15
SETTINGS.................................................................................................................................................................................................... 16
8.2.1 Temperature ................................................................................................................................................................................. 16
8.2.1.1 Control Mode ..................................................................................................................................................................................................... 17
8.2.1.2 Fan Speed ............................................................................................................................................................................................................ 19
8.2.1.3 Air filters .............................................................................................................................................................................................................. 19
8.2.1.4 Status .................................................................................................................................................................................................................... 22
8.2.1.5 Calibration .......................................................................................................................................................................................................... 22
8.2.1.6 Digital Input ....................................................................................................................................................................................................... 23
8.2.2 Touch Screen ................................................................................................................................................................................. 24
8.2.2.1 Options.................................................................................................................................................................................................................. 24
8.2.2.2 Brightness ........................................................................................................................................................................................................... 24
8.2.2.3 Visual Effects ...................................................................................................................................................................................................... 25
8.2.2.4 Date & Time........................................................................................................................................................................................................ 25
8.2.2.5 Alarms................................................................................................................................................................................................................... 26
8.2.2.6 Data Logging ..................................................................................................................................................................................................... 26
OVERVIEW.................................................................................................................................................................................................. 31
ICON AND GLANCE MODE VIEW ............................................................................................................................................................. 31
INFO PAGE .................................................................................................................................................................................................. 31
9 CALIBRATION......................................................................................................................................................................... 32
CAGE-CALIBRATION ................................................................................................................................................................................. 32
10 ENCLOSURE STANDARD COMPONENTS ................................................................................................................... 34
11 CLEANING & MAINTENANCE ......................................................................................................................................... 42
CONTROL UNIT.......................................................................................................................................................................................... 42
ENCLOSURE ................................................................................................................................................................................................ 42
FILTERS REPLACEMENT .......................................................................................................................................................................... 43
12 SUPPORT ............................................................................................................................................................................. 45
13 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 46
14 TROUBLESHOOTING ........................................................................................................................................................ 47
15 FIGURE LIST ........................................................................................................................................................................ 48
16 MANUAL REVISION TABLE ............................................................................................................................................ 49

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1 Preface

The H201-T-UNIT-BL, together with the H201-ENCLOSURE, is designed to maintain all the thermal
conditions required around your microscope, thus enabling to carry out prolonged observations on biological
specimens. The system controls the temperature in the range of 25-45°C.
The system is composed of the enclosure surrounding the microscope, H201-ENCLOSURE, which design
depends on the user’s microscope, the Temperature Control Unit, H201-T-UNIT-BL with the Heating Box, the HM-
VF composed by a humidity module and a water trap (purchased separately)1, a fine gauge thermocouple to
monitor the temperature of a blank sample or Reference Well located in close proximity to the experimental one,
a thermistor to monitor the cage temperature or the Room Temperature and the OKO-TOUCH touch screen control
panel (to be ordered separately).
H201-T-UNIT-BL is compatible with any Bold Line Gas Controller Temperature and Gas controllers are
stackable and connectable through a bus connector (see paragraph 7.1).
H201-T-UNIT-BL is operated via the OKO-TOUCH touch screen control panel (to be ordered separately).
OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition data logging is possible via SMART-BOX, via DATA-LOG Okolab software and via following
third-party Imaging software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN
Black software is available upon request.
Okolab DATA LOG is an optional software allowing logging temperature data or modification of selected
parameters. DATA LOG can be installed on your PC; a mini USB connection to the Temperature Control Unit allows
data download.
Okolab Bold Line controllers are also capable to receive TTL input (requires to order separately TTL-
CABLE).
The H201-T-UNIT-BL can be equipped with the H201-T-FILTER-BOX (optional) that assembles with the
Heating Box to ensure a dust and bacteria free environment inside the enclosure. In this case, H201-T-FILTER-BOX
is assembled with the H201-FILTER KIT, a kit composed by a Prefilter and a HEPA filter (see Figure 20 in paragraph
8.2.1.3).
We recommend reading carefully this manual to familiarize yourself with the functions and the operation
of H201-T-UNIT-BL before use.

1As Humidity Module you can purchase also HM-ACTIVE or HM-STANDALONE systems. In this case please refer to their own
manual for humidity-related instructions.
3
2 Symbol description

This paragraph describes the symbols used in the manual and on the product label.
Symbols used in this manual
The following symbols identify important information to note:

CAUTION or WARNING: this symbol warns you about the risk of electrical shock.

CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.

Tip ► Supplies you with helpful suggestions.

Note ► Supplies you with important information to successfully setup and use the instrument.

Symbols on the product label

CE MARKING: this symbol indicates product compliance with EU legislation.

PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid
waste.

This symbol indicates the product production date.

This symbol indicates the manufacturer data.

This symbol warns you to read the user manual before starting the device.

This symbol indicates the protection degree against ingress of solids or liquids inside the product.

IP 30

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3 Safety Notes

In order to achieve maximum performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.

− The equipment must only be used as intended and as described in this Manual.
− Equipment should be operated only by technically qualified personnel.
− Do not start up the equipment if some of its parts are damaged.
− This instrument is not intended for use in locations subject to flammable or explosive gases.
− Transport the equipment with care.
− Equipment and its internal parts can be damaged by dropping and by shock.
− Not following these instructions can result in damage or breakdown of the device and its
accessories.
− Avoid rapid changes in ambient temperature which may cause condensation, avoid direct air
draft from air conditioner, exposure to direct sunlight and excessive heat accumulation.
− The products labels can be found on the bottom panel of the Main Unit.
− Do not disassemble any part of the system.
− Do not disconnect cables while in operation.
− Do not use a volatile solvent such as paint thinner to clean the instrument because deformation
or discoloration may occur.
− Use a soft, dry cloth to remove stains from the instrument.
− Do not exceed voltage indicated in this manual and on the product label.
− Avoid excessive induction noise, static electricity and magnetic fields.
− Do not expose this instrument to rain or moisture.
− Prevent throttling and kinking of tubing.
− Check tubing time to time for possible material usage.
− Check that all tubing is well inserted into the connectors so they cannot slip off
− This device is not designed for use for medical applications.
− Power cord of unit should be unplugged from electrical outlet when left unused for a long period
of time.

− Do not start up the equipment if the supply cable is damaged.

− Connect the equipment only to grounded mains power socket.
− Do not open the unit. Do not remove cover or back.
− Prevent metal fragments or lead wire scraps from falling inside instrument to avoid electric
shock, fire or malfunction.
− No user serviceable parts inside.
− Unit should never be used where it can fall or be pushed into water.

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 International caution symbol marks this device. It is important to read the “Safety Notes” before
installing, using and commissioning this device, as the notes contain important information relating
to safety and EMC. Not following these instructions can result in damage or breakdown of the device
and its accessories.

We reserve the right to make technical variations.

IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE
OF ANY PRODUCT.

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4 Supplied equipment

H201-T-UNIT-BL includes:

1. T-Unit (pointer 1 in Figure 1)

2. Heating Box (pointer 2 in Figure 1)
3. Fine Gauge Thermocouple (pointer 3 in Figure 1). To measure specimen temperature
4. NTC Thermistor (pointer 4 in Figure 1). To measure ambient temperature
5. #1 24V-DC Power Adapter (pointer 5 in Figure 1). To connect the Temperature Control Unit

7 6

2 13 1

10

12 4 3

Figure 1. H201-T-UNIT-BL equipment overview.

5 NOT supplied equipment

H201-T-UNIT-BL can be equipped with:

1. Microscope enclosure (pointer 6 in Figure 1)
2. HM-VF, standard humidity control, that includes:

• Humidity Module (pointer 7 in Figure 1)

• Water Trap (see paragraph 10 Figure 56)
• TUBE G (x1). Silicon tube, 3 mm ID, connected by a plastic connector with a silicon tube 2 mm ID. The
3 mm ID silicone end must be connected to the Output Water Trap, while the 2 mm ID silicone tube to
the Chamber Incubator. See Figure 66.
• TUBE H (x1). Silicon tube, 3 mm ID. To connect the Humidity Module Output to the Water Trap Input.
See Figure 66.
• TUBE I (x1): Polyurethane tube, 4 mm OD, 45 mm long. To connect the Input of the gas coming from
the Gas Mixer to the Humidity Module. See Figure 66.

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3. 8-pins cable (x1). To connect the connector labeled ‘Control Unit’ on the H201 T Heating Box with the
connector labeled ‘Heating Box’ on the Temperature Control Unit (see paragraph7.1).
4. 3-pins – Flow Sensor Cable (x1). To connect the flow sensor attached on the Blue Side of Heating Box
to the connector labeled ‘Air flow sensor’ on the Temperature Control Unit (see paragraph 7.1).
5. Calorflex tubes (x4) (pointer 10 in Figure 1). To connect the Heating Box to the enclosure. Use two tubes
labeled red (TUBE-DE) to attach the RED colored side of the Heating Box to the hoses of the enclosure
labeled red. Use the two tubes labeled blue (TUBE-DF) to attach the BLUE colored side of the Heating Box
to the hoses of the enclosure labeled blue (see paragraph 10 Figure 57 and Figure 58).
6. Power supply cable. To connect the Heating Box.
7. Okolab incubating chamber (see pointer 12 in Figure 1) with lid and base, chamber riser with one or
more plate adapters.

8. OKO-TOUCH (see point 13 in Figure 1), including:

− Touch Screen Interface

− #1 RS232 Serial cable. To connect the Touch Screen to the T-Unit
− MINI-USB-OTG cable. To connect a USB pen to the OKO-TOUCH for data download.

Touch Screen Interface

RS232 Serial cable

Figure 2. Oko-touch components.

Optional Equipment

• H201-T-FILTER-BOX , including:

1. H201-T-FILTER-KIT, Pre filter and HEPA filter

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 H201-T-FILTER-BOX

Figure 3. H201-T-FILTER-BOX.

• DATA LOG (Optional-to be purchased separately). Software used to log, view, and store data from a
PC. It may be used to change parameters of the controllers via PC. Data can be exported in .xlsx format.

Figure 4. DATA LOG software.

• SM-BL Smart Box. To store data, to operate the controllers via web/smartphone/tablet and to allow
remote assistance for service.

6 Equipment Description

A detailed description of the H201-T-UNIT-BL rear is provided in the following list, which refers to the
pointers in Figure 5:

1. OKO-TOUCH RS232 serial port.

2. Power Input.
3. Heating Box connector.
4. Air Flow Sensor connector.
5. USB port.
6. General Purpose Input Output (GPIO) port.
7. NTC Thermistor port.
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8. Gauge Thermocouple port.

2 3 4 5 6 7 8

Figure 5. Control Unit Overview

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7 Installation
The following paragraphs will illustrate how to install and to use the Temperature Control Unit.
H201-T-UNIT-BL. Installation guide

The following instructions shall give you the possibility to install the unit quickly. For safe operation
of the unit it is absolutely necessary to read carefully all the instructions and safety notes.

Fine gauge
thermocouple

8-pins
cables

NTC Thermistor

3-pins
cables

Power
cord

Sensor cable
Electrical Connection

Heating Box

Figure 6. H201-T-UNIT-BL. Standard Connection

1. Connect the Heating Box to the port labeled ‘Heating Box’ on the rear panel of the Temperature Control Unit
(see Figure 6).
2. Plug the NTC Connector on the port labeled ‘Ambient Temperature’ located on the rear panel of the
Temperature Control Unit (see Figure 6). This temperature sensor must be placed in air close to the
illumination pillar in proximity to the microscope stage. This thermistor monitors the air temperature inside
the cage. The NTC thermistor can be also used to monitor the room temperature when working in Sample
Feedback Mode. In this case, you have to place it outside the cage.

3. The Fine Gauge Thermocouple (green) plugs into the green port labeled ‘Reference Temperature’ located on
the rear panel of the Temperature Control Unit (see Figure 6). When operating the device in ‘Sample
Feedback Mode’ place this thermocouple in a Reference Well filled with water. Make sure that the end of the
Fine Gauge Thermocouple is fully immersed.

Tip ► The Fine Gauge Thermocouple may be placed into a Reference Well if running the “Sample Feedback
Mode” (see paragraph 8.2.1.1). The Fine Gauge Thermocouple may also be used to calibrate the Microscope
Incubator temperature if running the “Cage Feedback Mode” (see paragraph 9.1). The Fine Gauge
Thermocouple may be left in air if the system is operated in ‘Cage feedback mode’ (see paragraph 8.2.1.1)

4. Install the microscope enclosure (purchased separately) following the instructions of its dedicated booklet
and reading the paragraph 10 for the positioning of some its components.
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5. Connect the touch screen interface OKO-TOUCH to the Temperature Control Unit using the serial cable
provided (see Figure 7).

H201-T-UNIT-BL

OKO-TOUCH

Figure 7.OKO-TOUCH Connection

6. Connect the 24V-DC Power adapter to the Temperature Control Unit.

7. To switch H201-T-UNIT-BL on use the power button on OKO-TOUCH (see Figure 11). A small green LED light
will blink on the front of the Temperature Control Unit. This means that the system is initializing.

Tip ► Wait until system has reached the steady state before loading your samples.

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 How to connect H201-T-UNIT-BL to a BOLD LINE Digital Gas Controller

H201-T-UNIT-BL can be used as a stand-alone device or in combination with any one of the Bold Line Gas
Controllers. Figure 8 shows how to assemble H201-T UNIT-BL, for example, with a CO2-O2 Unit BL [0-10; 1-18].

a. CO2-O2 UNIT-BL [0-10; 1-18] and b. CO2-O2 UNIT-BL [0-10; 1-18] and
H201-T-UNIT-BL. Units rear panels. H201-T-UNIT-BL. 3D view.
Figure 8. CO2-O2 UNIT-BL [0-10; 1-18] and H201-T-UNIT-BL

The Gas Controller must stack on top of the Temperature Control Unit by lining up the bus ports located
on the top and bottom surface of each. When the units are properly connected the Temperature, CO2 and O2
parameters will appear on the Touch Screen interface Home page. If not all the parameters appear on OKO-TOUCH
Home Page then the Control Units are not properly connected. Please check that the bus ports are properly aligned
with each other.

If you have the CO2 Controller and a Temperature Control Unit, it is essential to read both
this manual and the Temperature Control Unit Manual to familiarize yourself with the
functions and the operation of the devices before use.

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8 User Interface
This chapter describes the user interface of the H201-T-UNIT-BL.
Home page

1 2 3 4

10

Figure 9. Homepage of the H201-T-UNIT-BL Controller Touch Screen Display

1. Status LED

2. Temperature set point (see paragraph 8.1.1 to learn how to change the set point value)

3. Temperature current value

4. Minimun and Maximum Temperature reached within the time frame set in the Options subpage
(see paragraph 8.2.2.1)

5. Chart history length set at 1 hour

6. Display mode. Touch here to switch display mode

7. Overview page

8. Settings. Press here to access system options and settings

9. Home. To open the homepage

10. Product info. Press here to know generic info about OKO TOUCH and H201-T-UNIT-BL and
running time

Tip ► The Touch Screen is pre-set at the following temperature: 37°C. Once turning the Touch Screen on it
will start operating to reach this set-point value.

8.1.1 How to enter the set point temperature

To input a new set-point temperature, touch the corresponding tab, as indicated in Figure 10 a.

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 1

a b

Figure 10. How to change the Temperature set point (a – b)

The set point regulation page will appear as in Figure 10 b. You can modify the set point by clicking on +
and -. Once you have input the new set point temperature, press “Set” to save or “Cancel” to undo.

Tip ► After any change in the set point value the controller enters into a transient regime, the Status
Indicator and the Top LED turn yellow (see paragraph 8.1.2). During the transient regime, the controller will
not trigger on alarm.

8.1.2 Controller Status: colours led and meaning

The H201-T-UNIT-BL can assume four different status, which are represented by the colors assumed by
the Status Indicator (pointer 1 in Figure 9) and by the TOP LED (if enabled, as indicated in paragraph 8.2.2.3):

The GREEN color indicates that the set-point value has been reached (within the
tolerance defined in the alarm subpage, see paragraph 8.2.2.5) and that the system is working
properly.
Controller Status: NORMAL

The YELLOW color indicates that the controller is in transient regime. The Yellow light
will appear after the controller is turned on and after any set point chance. The system is working
properly, it is not in alarm and no action is needed. As soon as the H201-T-UNIT-BL stabilizes the
temperature, the Controller Status changes to NORMAL and the color turn GREEN. If the H201-
T-UNIT-BL cannot stabilize the temperature of one or both heating devices within the maximum
time defined by the operator (see paragraph 8.2.2.5), the Controller Status changes to ALARM
and the color turn ORANGE.
Controller Status: TRANSIENT

The ORANGE colour indicates that the current Temperature value is out of the tolerance
defined in the alarm subpage (see paragraph 8.2.2.5). The controller triggers in Alarm.
Controller Status: ALARM

The RED color indicates that there is a problem with the unit itself (for example a
Temperature sensor is broken). The system is in alarm. Turn the system off, wait for 5 minutes,
and turn it back on. If the color is still red, contact Okolab at www.oko-lab.com for support.
Controller Status: ALARM

Tip ► The Top LED (see Figure 11) follows the same color code as the status LED.

To activate the Top LED, follow the instructions reported in paragraph 8.2.2.3.

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 Top LED

Power Button

Figure 11. Top LED and Power Button.

Settings

8.2.1 Temperature
To choose and insert the proper operation parameters, follow the instruction below:

1. Press on the Settings icon (see Figure 12 a) to enter the Settings menu.

2. Press on the Temperature icon to enter the temperature settings menu, as shown in the Figure 12 b.

a b

Figure 12. Temperature Settings (a – b)

The Temperature settings menu has 5 items, as shown in Figure 13.

Figure 13. Temperature Settings submenu

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8.2.1.1 Control Mode
The Temperature Control can run in two different modes: Sample or Cage Feedback Mode. Press on

Control Mode icon (see Figure 14 a) to choose which one you want to use, Sample or Cage Feedback
Mode. Figure 14 b shows how to select Cage Feedback Mode.

a b

Figure 14. Chamber Mode Settings (a - b)

In this configuration the temperature of the air inside the cage is controlled, precisely in the position
where you have placed the NTC thermistor. It is strongly recommended to attach the NTC thermistor close to the
sample chamber, at right on the stage or on the top condenser (use a standard adhesive tape to fix the sensor on
those parts). The advantage of this solution is that you don’t have to manually stick the fine gauge thermocouple
into a reference well. Once selected the CAGE feedback mode, the operator should calibrate the difference of
temperature between the air inside the enclosure (where the NTC thermistor has been placed) and the specimen
temperature (by sticking the fine gauge thermocouple into a reference well inside the Chamber Incubator), so to
find for instance that to keep the specimen temperature at 37°C, the cage temperature has to be at 38°C (see
paragraphs 8.2.1.5 and chapter 9). This means that when regulating the set-point, given that in this mode you
control the Cage’s temperature, this has to be set at 38°C to keep the specimen at 37°C. This control mode is well
suited for multi-user tasks where users don’t want to take care to correctly position the fine gauge thermocouple
into a reference well inside the chamber incubator; however, it is based on a calibration between cage and
specimen, therefore cannot be considered an active thermal control on the sample

Tip ► Advantages of this Control Mode are: fast experiment start up and no specific action required in multi
user applications. The disadvantage is that it needs calibration between cage and specimen. The temperature
difference between the cage and the sample sensors depends on many factors like: the ambient temperature,
the enclosure design, the sensors position, but if these ones don’t change, the temperature difference doesn’t
change.

Figure 15 b shows how to select Sample Feedback Mode.

a b

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 Figure 15. Sample Mode Settings (a - b)

In this configuration, every time you run an experiment, you must place the Fine Gauge Thermocouple
(thin green wire) into a Petri dish or well adjacent to your sample (from here on this will be referred to as
Reference Well). Fill the Reference Well with distilled water to a level allowing full immersion of the fine gauge
thermocouple (see Figure 16). When possible close the petri with its own plastic lid (or with SENSOR LID, see Tip
below) and place it in the appropriate holder. Using a Reference Well in close proximity to your Experimental Well
provides active Sample Feedback Temperature Control. This ensures that your sample will be within 0.1°C from
the temperature set point regardless from room temperature variations.

Figure 16. Free Sensor attached on the internal base of the Petri Dish

Tip ► It is recommended using an Okolab SENSOR LID (available for purchase) to secure the Fine Gauge
Thermocouple in place in the Reference Well during calibration or operation in Sample Feedback Mode (see
Figure 17).

Sensor lids are available for the following: 35 mm, 60 mm Petri-dish, chamber slide and chambered cover
glass. Sensor Lids are ordered separately using the codes in following table.

SENSOR LID CODES

SENSOR LID-35 Temperature sensor lid. To be used in specimen feedback in a 35 mm Petri Dish

SENSOR LID-60 Temperature sensor lid. To be used in specimen feedback in a 60 mm Petri Dish

Temperature sensor lid. To be used in specimen feedback in chamber slides and chambered
SENSOR LID-GS
cover glass
Table 1. Sensors lid codes

SENSOR LID

Fine Gauge
Thermocouple

Figure 17. SENSOR LID-35. For 35 mm Petri-dish

Tip ► Advantages of Sample Feedback Control Mode are: direct monitoring and active control of sample
temperature and independence from room temperature variations. The disadvantage is that it requires
manual operation for correct placement of the fine gauge thermocouple in a Reference Well.
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8.2.1.2 Fan Speed

The Heating Box has an internal flow sensor that allows regulating the speed of the Air being blown inside
the enclosure. An increased speed allows a quicker start-up and a more uniform thermal distribution inside the
enclosure, a low speed allows instead a smooth operation in applications particularly sensitive to vibrations.

You can set the Air Flow value by pressing on Fan Speed icon (see Figure 18 a). Then you can change
the value in the range 35-100 m /h (see Figure 18 b). Only when you use the H201-FILTER-BOX, the working range
3

of the Air Flow becomes 35-70 m3/h, see paragraph 8.2.1.3.

a b

Figure 18. Fan speed setting (a – b).

You can also activate the ‘Fast Heating’ button (see Figure 19), which enables a quick start-up every time
you turn on the system. The ‘Fast Heating’ accelerates the transient time.

Figure 19. Fast Heating Activation.

8.2.1.3 Air filters

If you have purchased also H201-T-FILTER-BOX, a module containing a filters kit composed by a prefilter
and a HEPA filter (High Efficiency Particulate Air Filter) has been added to the Heating Box, see Figure 20. In this
case, Okolab recommends activating the ‘Filter Monitoring’ in the Air Filters subpage.

Press on Air Filters icon (see Figure 21 a), then active “Filter Monitoring” (see Figure 21 b).

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 H201-T-FILTER-BOX

Figure 20. Heating Box with H201-T-FILTER-BOX

a b

Figure 21. Filter Monitoring activation (a – b).

When you active the ‘Filter Monitoring’, the counting of the usage days (indicated with ‘Update Time’)
starts. The maximum usage time for the Prefilter is 180 days, while for the Hepa Filter is 360 days.
When the usage time exceeds these values, the warning icons appear in the ‘Homepage’ and in the
‘Overview’: icon A in Figure 22 for HEPA filter, icon B in Figure 23 for Prefilter.
If you press on the Prefilter or the HEPA icon (see Figure 24 a and Figure 25 a), the warning shown in
Figure 24 b and Figure 25 b will appear. These warning messages and icons allow you to remember that you should
replace one or both the filters in order to avoid contaminations of the samples. See paragraph 11.3 for the Filters
Replacement.

A A

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 Figure 22. Home Page and Status Page when the usage time of the HEPA filter exceeds the maximum time of 360 days

B B

Figure 23. Home Page and Status Page when the usage time of the Prefilter exceeds the maximum time of 180 days

a b

Figure 24.Warning for Prefilter replacement.

a b

Figure 25. Warning for HEPA replacement

IMPORTANT: when you use the H201-FILTER-BOX, the working range of the Air Flow becomes 35-70
m3/h, if you set the Air Flow set point at a value more than 70 m3/h the message shown in Figure 26 will appear

21
 Figure 26. Air Flow Warning message when using Air Filters

8.2.1.4 Status

Press on the Status icon to open the Status page (see Figure 27 a). The Status Temperature page will
open (see Figure 27 b). On this page you can see the status of the Temperature Control Unit, with a summary of all
parameters allowing you to check that all is working properly. You may have to refer to this window when asking
for Okolab technical support.

a b

Figure 27. Status page (a – b)

8.2.1.5 Calibration
For maximum of accuracy when using “Cage Feedback Mode”, H201-T-UNIT-BL allows to calibrate the
system in your lab at your Room Temperature. To do so, read the paragraph 9 and then follow the instruction
below:

1. Press on the Calibration icon (see Figure 28 a)

2. Press the Adjust button when the sample temperature is stable, as shown in Figure 28 b; the system will
calculate the offset value to apply to the Cage Temperature to have the sample at the desired temperature.

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 Figure 28. Cage Calibration (a-b)

Tip ► Make sure the temperature values are stable before starting the cage calibration as suggested in
dialog opening when pressing “Adjust” (see Figure 29).

Figure 29. Cage calibration warning

8.2.1.6 Digital Input

Digital Input feature is available only for TTL ready (optional) controller.
TTL feature allows to change setpoint between two specified values using a TTL signal (see Figure 30 b).
The TTL is a digital input for our Units and has only two values: High and Low. The setpoint values for High TTL
value and for Low TTL value can be set using OKO-TOUCH, via GPIO connector.
When TTL Control is enabled, the user cannot change the setpoint using the dialog in the main page. In
this case, the setpoint value is read by the external TTL signal.

Press on the Digital Input icon to enable digital input feature, as shown in Figure 30 a and b.

a b

Figure 30. Digital input ( a- b)

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8.2.2 Touch Screen

Press on the Settings icon (see Figure 31 a), then press on the Touch Screen icon (see Figure
31 b) to access to touch screen settings.

a b

Figure 31. Touch screen settings (a- b)

8.2.2.1 Options

Press on the Options icon (see Figure 32 a) to set the scale of the X axis (time duration) of the graphs
displayed in the main page and the buzzer sound frequency (see Figure 32 b).

a b

Figure 32. Touch Options (a – b)

Press and slide your finger along the setting bars or simply click +/- to set desired values, then press “Save”
(see Figure 32 b).

Tip ► Recommended value for the chart history length is 60 or 120 minutes.

8.2.2.2 Brightness

Press on the Brightness icon (see Figure 33 a) to enter the display brightness setting, This page
allows you to increase/reduce the brightness of the touch screen, scroll the bar or press the +/- buttons (see Figure
33 b).

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 a b

Figure 33. How to enter in Brightness page

8.2.2.3 Visual Effects

To enter the display visual effects menu, press on the Visual Effects icon (see Figure 34 a).

a b

Figure 34. Visual effects page (a – b)

The Top LED settings allows you to when the Top LED should be illuminated (see Figure 34 b): if you
select Never, the Top LED will never illuminate, if you select On Alarm, the Top LED will illuminate only when the
H201-T-UNIT-BL is in Alarm Status (see paragraph 8.1.2), if you select Always, the Top LED will always illuminate.

8.2.2.4 Date & Time

The first time you turn the system on, it is important to set date and time. To set date and time press on

the Date & Time icon (see Figure 35 a), then use the + and – to set correct date/time and then press “Save”
(see Figure 35 b).

a b

Figure 35. Touch screen settings. Date and Time (a – b)

25
8.2.2.5 Alarms

Press on the Alarms icon as shown in Figure 36 a. Then press on the Temperature icon (see
Figure 36 b).

a b

Figure 36. How to enter in Alarms page (a – b).

Tip ►Flag “Buzzer” if you want the Alarm to be acoustical as well rather than just being displayed.

The page shown in Figure 37 will open and you can choose the “Temp Deviation” and “Temp Time”.

Tip ►”Temp Deviation” is the value defining the allowed tolerance from the set point. “Temp Time” is the
time for which the temperature may remain outside the allowed tolerance before the controller triggers in
alarm.

Figure 37. How to set the Deviation and Time Alarm

Figure 37. In this example the following settings are displayed (valid only after the system has reached its
stationary state): if Temperature Deviation from set point is 1°C or greater (i.e. if set point temperature is 37°C
and the temperature reaches a value equal or less than 36.0°C or equal or more than 38.0°C) for a period of time
equal or longer than 15 minutes (“Temp time“ set in this example) then the system triggers in alarm.

8.2.2.6 Data Logging

H201-T-UNIT-BL touch screen interface, OKO-TOUCH (see Figure 2), is equipped with on-board memory
for data logging and storage. A simple routine allows downloading data to USB drive or to PC.
In order to log and then download the logged data, connect a USB drive (not supplied) to OKO-TOUCH,
using MINI-USB-OTG cable (provided), as shown in Figure 38.

26
 MINI-USB-OTG

USB Port

USB Drive

OKO-TOUCH

Figure 38. USB drive connection

To activate the logging, follow the steps indicated below:

1. Press on the Settings icon (Figure 39, a) and press on the Touch screen icon on the
window that opens next (Figure 39, b).

a b

Figure 39 (a) How to enter the setting menu; (b) how to enter the Touch Screen menu

2. Press on the Logging icon and flag Internal memory in the page that appears, see Figure 40
a and b.

Tip ► When activating the logging on the internal memory, you can access the Logging page also by pressing

the activated logging icon on the Homepage, as shown in Figure 41

27
 a b

Figure 40. Logging. (a) How to enter in the logging menu; (b) logging in internal memory

Figure 41. How to access the Logging page from the Homepage

3. Press on the icon (see Figure 42 a) to insert the Time Interval i.e. the frequency with which
you want to record a data point, as shown in Figure 42 b. In this example a data point is logged
every 30 seconds, you can change Time Interval in the range 1-60 seconds.

a b

Figure 42. How to set the logging time (a – b)

4. Choose how you want to organize the data when downloaded, by pressing on Day, Week or
Month, see Figure 42 (a).

Tip ►If you select Day, the data will be split in files, each one containing the data of one day. If you select
Week, the data will be split in files, each one containing the data of one week.

5. Write the file name suffix to be reported in the downloaded files by pressing on the keyboard

icon , see Figure 43 a.

28
 6. Then press Save, see Figure 43 b.

Tip ►With the selections as in Figure 42 and in Figure 43, the data will be downloaded on the USB drive as
shown in Figure 44.

a b

Figure 43. Data Logging. How to define the suffix for the downloaded files (a – b)

Figure 44. Downloaded files on the pen drive

Note ► If you press on the screwdriver , see Figure 45(a) you can view the logging starting date, the
available memory and the latest download, see Figure 45(b).

Note ►The available memory depends on the time interval you have inserted in the Logging page. The
default logging Time Interval is 30 seconds.

a b

Figure 45. Internal memory status. (a) Logging page settings; (b) Internal log page settings

To download the data, press on To USB or on Erase if you want to delete the data, see Figure 46 b.

29
 a b

Figure 46. Download to USB

You can activate the logging also on the USB drive, by flagging on USB drive, see Figure 47(a).

a b

Figure 47. Logging on USB flash drive. (a) USB drive selection; (b) Reminder to connect the USB drive

In this case, OKO-TOUCH warns you that a USB drive must be connected to the USB port, see Figure 47(b),
and will store the data on the USB drive.

Figure 48. Logging to USB drive

Note ► When connecting the USB drive to OKO-TOUCH, a USB drive icon appears on the Homepage. If
you activate the logging on USB drive, a red dot appears on the USB drive, to remind that the USB drive should
not be disconnected while data logging is ongoing see Figure 48.

30
 Overview

Press on the icon to open the overview page as show in Figure 49 a.

a b

Figure 49. How to enter in the Overview Page (a – b).

Note ►This page contains data useful for technical/control reasons. In case you requested support from one
of Okolab engineers you may be asked for some of these data

Icon and Glance Mode View

The H201-T-UNIT-BL features two display modalities: Icon mode and Glance Mode, as shown in Figure

50, a and b. You can swap between the two display modalities by pressing on icon , as shown in Figure 50 a.

a b

Figure 50. Display modalities: Icon Mode (a); Glance Mode (b).

Info page

Press on the icon to access the Info Page, as shown in Figure 51. This page contains the information
related to the OKO-TOUCH and to the H201-T-UNIT-BL version (as shown in Figure 52 a and b).

31
 Figure 51. Info page selection

a b

Figure 52. Info page OKO-Touch and H201-T-UNIT-BL (a – b).

Tip ►Please have this information handy when contacting Okolab for support

9 Calibration
Cage-Calibration

Carefully read the instructions given in this paragraph before starting the Cage Calibration.

Note ► It is important to monitor the room temperature during the calibration, as well as reducing air drafts
and forced convection. If possible divert any draft coming from the air conditioning system in the room, from
the microscope incubation system.

The goal of this procedure is to adjust the temperature offset of the Cage temperature hence maintaining
the sample at the desired temperature (e.g. 37°C) during imaging. Before starting the Cage -Calibration:

1. Plug the green Fine Gauge Thermocouple in the port labeled “T sensor” (see Figure 5 pointer 8) located in
the rear panel of the Temperature Control Unit

2. Insert the Fine Gauge Thermocouple into the chamber by threading the wire through one of the screw holes
on the chamber riser
32
3. Secure the end of the Fine Gauge Thermocouple to the bottom of a 35mm or similar Petri dish using some
tape making sure to avoid covering the head of the probe (see Figure 16).

4. Fill the Petri dish with distilled water to a level sufficient to fully immerse the Fine Gauge Thermocouple.
This is a Reference Well simulating experimental conditions. Place the lid on the Petri dish, and insert the
dish in the incubation chamber. You can also use SENSOR LID-# to close the dish (see the “Tip” below).

Tip ► It is recommended using an Okolab SENSOR LID (available for purchase) to secure the Fine Gauge
Thermocouple in place in the Reference Well during calibration or operation in Sample Feedback Mode (see
Figure 17 and Table 1).

5. Go in Control Mode page as shown in Figure 13 and enable “Cage Mode”

6. Wait until the system reaches the steady state (temperature indicator status is green).
7. When the Fine Gauge Thermocouple reads a stable temperature open the Calibration page as shown in
Figure 28 and press “Adjust” and wait until the temperature measured by the Fine Gauge Thermocouple is
stable. If the temperature measured by the Fine Gauge Thermocouple is now close to the set point, then the
Calibration is complete. If the temperature measured by the Fine Gauge Thermocouple is far from the set
point, repeat the steps above until the temperature measured by the Fine Gauge Thermocouple reaches the
set point temperature.
8. You can remove the Fine Gauge Thermocouple from the Reference Well petri-dish inside the incubating
chamber.

33
10 Enclosure standard components

H201-T-UNIT-BL includes the enclosure (cage) surrounding the microscope (purchased separately). The
enclosure depends on the user’s microscope therefore it can be different for each microscope. There are some
parts of the enclosure that are, however, the same for all. The standard parts are shown in the following images.

A. Humidity Module (included in HM-VF) positioning on the enclosure top panel. You can insert this
part of the HM-VF also at the end of the assembly of the whole cage. See Figure 53.

Figure 53.HM-VF inserting in the appropriate slot

Tip ► The Humidity Module allows to humidifying the mixed gas coming from the Gas Mixer by a permeable
water membrane. The dry gas flows through this permeable membrane immersed in water before arriving
to the chamber incubator. The Humidity Module has to be filled with distilled water until to cover entirely
the tubing into the reservoir, this operation can be done before inserting it into its housing or after the
insertion, you have to lift the black plug only (see Figure 54).

Fill the Humidity Module

with distilled water

Permeable Water
Membrane

Figure 54. How to fill the Humidity Module.

Note ► This particular component is not present if you have purchased HM-CTIVE as Humidity Module. In
this case this bore is covered by a plug while the system configuration becomes the one shown in

B. Water Trap (included in HM-VF) positioning inside the enclosure. You can insert this part of the HM-
VF or before mounting the frontal panel or after have mounted the frontal panel simply opening the slide
door on the left panel of the enclosure. See Figure 55.

34
 Figure 55. Water Trap inserting in the appropriate slot

Tip ►This component allows recovering the condensation water (HM-VF) or the water accumulated into the
permeable membrane tube (for HM-VF) in order to prevent the water reaches the chamber incubator. For
example, in the case of HM-VF, when you switch your gas mixer off, the gas stops flowing through the
permeable water membrane for a little time but the membrane continues to permeate the water and its little
tube fills. When you switch your gas mixer on again, the stored water is recovered into the water trap and it
does not reach the chamber incubator. The Water Trap is directly connected to the Humidity Module by the
TUBE H (for HM-VF, see Figure 66)

The Water Trap should not fill because the stored water evaporates when the system restarts in the standard
working condition. If it fills because of anomalous operating condition, you have to open it to empty it:

• Loosen the water trap cover screws (M2x8, A in Figure 56)

• Lift the cover and empty the water trap

Figure 56. How to empty the water trap

C. Calorflex tubes (x4) (see paragraph 5) provide connection between the Heating Box and the
Enclosure. The two tubes labeled red (TUBE-DE) allow to connect the RED colored side of the Heating
Box to enclosure hoses red labeled. The tubes labeled blue (TUBE-DF) connect the BLUE colored side of
the Heating Box to the enclosure hoses labeled blue.
35
 Connection with the Heating Box. Insert the hose bayonet connector ring that is on the end part of the tubes
labeled blue (TUBE-DF) on the hose bayonet connector flange mounted on the blue side of the Heating Box.
Then rotate the bayonet connector ring until the screws do not fit into their housings. Repeat the same
procedure for connecting the red colored tubes (TUBE-DE) on the red side of the Heating Box.

Figure 57. Connection with the Heating Box.

Connection with the Enclosure. Insert the hose bayonet connector ring that is on the end part of tubes
labeled blue (TUBE-DF) on the hose bayonet connector flange of the Enclosure labeled blue. Then rotate the
bayonet connector ring until the screws do not fit into their housings. Repeat the same procedure for
connecting the tubes labeled red on the enclosure hoses labeled red (TUBE-DE).

Figure 58. Connection with the Enclosure.

Tube Length Optimization. You can cut the tubes if they are too long for your configuration. Follow the
instructions below to cut the tubes:
1. Remove the black hose from gray tube. See Figure 59 a. Cut with the help of a scissors the gray tube to the
desired length, see Figure 59 b.

36
 Figure 59. Remove black hose and cut the tube (a – b)

2. Cut with the help of a nipper the metal wire inside the grey tube, see Figure 60 a. Hold the metal wire tip
with some scotch tape. See Figure 60 b.

Figure 60. Cut the metal wire and hold the metal wire with scotch tape (a – b).

3. Insert the black hose back in its position, see Figure 61

Figure 61. Insert the black hose.

Okolab suggests to cut the tubes in order to save space especially when:

1. the Heating Box is put on the same desk of the enclosure, very close to the enclosure (see Figure 62 for
Configuration A). Figure 62 a shows configuration where tubes have not been cutting. Figure 62 b shows the
space-saving after cutting the tubes.

37
 Figure 62. Before vs after cutting the tubes – Configuration A (a -b).

2. the Heating Box is put on a shelf placed just above the enclosure (see Figure 63 for Configuration B). Figure
63 a shows configuration where tubes have not been cutting. Figure 63 b shows the space-saving after
cutting the tubes.

Figure 63. Before vs after cutting the tubes – Configuration B (a -b).

You don’t need to cut the tubes when the Heating Box is placed under the enclosure’s level (see Figure 64
a for Configuration C) or above the enclosure but not precisely centered with it (see Figure 64 b for Configuration
D).

38
 Figure 64. Configuration C – Configuration D (a -b).

D. Chamber Incubator insertion inside the enclosure. You can insert the chamber incubator on the stage
before mounting the frontal panel or after have mounted the frontal panel simply opening the slide door
on the left or on the right panel of the enclosure. See Figure 65.

Tip ► Please refer to the chamber user manual in order to have more information on the chamber incubator
positioning on the stage.

Figure 65. Chamber Incubator insertion inside the enclosure

E. HM-VF tubing connection inside the Enclosure. If you have purchased also HM-VF, you have to connect
the tubes between the Water Trap and Humidity Module (Tube H), between the Water Trap and the
Chamber Incubator (TUBE G) and between the Input of the gas coming from the Gas Mixer and the
Humidity Module (TUBE I). See Figure 66.

Note ►The 3 mm ID silicon end of TUBE G must be connected to the Water Trap while the 2 mm ID silicon
end to the Chamber Incubator.

39
 TUBE G

TUBE I

TUBE H

Figure 66. Tubing connection inside the Enclosure with HM-VF.

F. HM-ACTIVE tubing connection inside the Enclosure. If you have purchased also a digital gas mixer
including HM-ACTIVE as humidity module, you have to connect the heated tube of HM-ACTIVE to the
Chamber Incubator. See Figure 67.

Figure 67. Tubing connection inside the Enclosure with HM-ACTIVE

40
G. On the all Okolab enclosures there are some standard components which allow simplifying the layout of
cables and pipes in your experimental environment. The Figure 68 shows for example an opening that
you can find on the back panels, Image 2, useful for the passage of the electrical cables of the microscope
or of the other components that you use for your experimental apparatus. In Image 3 of Figure 68 there
are the connector for the input of the gas coming from Gas Mixer and the opening where you can pass the
temperature sensors cables (the fine gauge thermocouple and the NTC thermistor). The Image 1 shows
the Heated Tube Input (a component of the HM-ACTIVE), this is tapped in the configuration H201-T-UNIT-
BL with HM-VF as humidifier.

1 2

Figure 68. Standard components on the enclosure panels.

41
11 Cleaning & Maintenance

Control Unit

To maintain proper Temperature Control Unit operation over time:

− Use a polishing cloth or dry cloth to wipe off dust and dirt.
− Never use thinners, benzene, solvents on or near the devices, since these could corrode their surfaces.
− To polish the Stage Incubator and the Humidifying Module, if it is present, you can use distilled water or alcohol
− Verify the status of all cables and if some cable is damaged, contact Okolab to receive assistance

Before cleaning the unit, pull out the mains plug.

Water must not be entered in the system.

Enclosure

The Cage enclosure is composed by several parts, in total five materials are present, see the list below:

Lexan (for transparent panels),

Acrylic (for obscuring panels),

Polycarbonate (for black seals for light obscuring)

Aluminum (for opening viewing window, not present in all the configurations)

Anodized Aluminum (for opening viewing window, not present in all the configurations)

In order to keep the Cage clean they are needed:

1. Wipe: Use a clean soft and dry sterilized cloth.
2. Disinfectant: Quaternary ammonium compound: Benzyl-alkyldimethyl chloride 1.3 mg/g. Effective
against hepatitis B, HIV, rota virus within 1 minute, mycobacterium within 5 minutes, bacteria, fungi
(Candida), influenza A virus (H5N1/H1N1) within 15 minutes. Make sure it won’t release any VOC after
application.

The procedure to clean the Cage is:

42
 1. Spray the Disinfectant on the chosen piece and let it act for 15 minutes.
2. Rub the piece gently with the Wipe.
3. If some halos are present on the piece (this may be the case of Acrylic), spray the Disinfectant again and
immediately remove it gently with the Wipe.

Note ► Please contact Okolab if you need further information about which Disinfectant and Wipe to use.

Filters Replacement

If you have purchased also H201-T-FILTER-BOX, a module containing a filters kit composed by a prefilter
and a HEPA filter (High Efficiency Particulate Air Filter) has been added to the Heating Box, as described in
paragraph 8.2.1.3. When activating the Filter Monitoring, the counting of the usage days, indicated with ‘Update
Time’, starts. The maximum usage time for the Prefilter is 180 days, while for the Hepa Filter is 360 days. When
the usage time exceeds one or both these values, you should replace them in order to avoid contaminations of the
sample.

Before opening the H201-FILTER-BOX, make sure that the Heating Box is unplugged from an outlet.
Before opening the H201-FILTER-BOX to change the Prefilter or the HEPA Filter wear personal
protective equipment, breathing mask and gloves.

Follow the indications below for the filters replacement:

1. Open the two soft draw latches on the sides of the H201-FILTER-BOX, see A in Image 1 Figure 69
2. Slip off the frontal panel of the H201-FILTER-BOX and pull out the prefilter and HEPA filter. See Image 2 and
3 of Figure 69.
3. Replace one or both the filters that you need to replace with the new filters.
4. Reassembly the H201-FILTER-BOX.

43
 A

1 2

HEPA FILTER

PREFILTER

Figure 69. Filters Replacement

After replacing one or both the filters, do not dispose it/them as urban solid waste. The filters must
be treated as special waste.

44
12 Support

In order to send directly an email to the Okolab staff, you can use the QR codes shown in the following
images:

• For commercial info: • For technical info:

Figure 70. QR code for commercial info contact Figure 71.QR code for technical info contact

To contact one of our engineers please see below, write to [email protected] or contact us through
the live chat on www.oko-lab.com. You can request a remote support session anytime, also via video chat.
− Contact our technical support ([email protected]) by e-mail to take an appointment for the
web assistance.
Technical Support.
Please, do not hesitate to contact Okolab should you need any further commercial information or technical
support.

For HARDWARE SUPPORT: [email protected]

Phone +39 081 806 3470
Fax: +39 081 876 4410
Mobile: +39 348 96807 18

For COMMERCIAL SUPPORT WORLDWIDE: [email protected]

Phone +39 081 806 2624
Fax: +39 081 876 4410
Mobile: +39 348 96807 17

For COMMERCIAL SUPPORT US&CANADA: [email protected]

For COMMERCIAL SUPPORT CHINA: [email protected]

45
13 Technical Specifications

H201-T-UNIT-BL - Technical Specifications

Cage Temperature Range: 25-50°C

Temperature Sample Temperature range: 3°C above ambient temperature to 45°C
Step size: 0.1°C
Accuracy:0.2°C in Sample Mode and in Cage Mode if room temperature remains within ± 1°C

Regime temperature time about 30 min

Operating Temperature 0°C ~ +55°C

Storage Temperature -5°C ~ +60°C

Operating Humidity 0-70%

Power Consumption 24 V DC 75 W max

Weight 1.5 Kg

Heating Box – Technical Specifications

Operating Temperature 0°C ~.+55°C

Storage Temperature -5°C ~ +60°C

Operating Humidity 0-70%

Power Consumption 230V AC 50 Hz Fuse 5AF 250V 5x20

800 W max

Table 2. Technical specifications.

46
14 Troubleshooting
We have collected in the table below some frequently asked questions, please contact Okolab if you need
support.
Symptom Probable cause Remedy

Supply cable disconnected Properly connect the cable

Device off
Supply cable damaged Substitute the cable

Thermocouple cable disconnected Properly connect the cable

No temperature displayed or Thermocouple cable damaged Contact Okolab to receive assistance

“nan" NTC Thermistor cable disconnected Properly connect the cable

NTC Thermistor cable damaged Contact Okolab to receive assistance

8-pins cable is not connected to Heating Box

Properly connect the cable
or T-Unit
Heating Box fan turned off
Replace the fuse in the Heating Box (see Technical
Blown Fuses
Specifications)

Power supply cable is not connected to

Properly connect the cable
Heating Box
Heating Box is cold
Replace the fuses in the Heating Box (see Technical
Heating Box Blown fuses
Specifications)

Heating Box fan is turned on, the

speed reported by OKO-Touch is
8-pins cable or Unit is damaged Contact Okolab for support
0 RPM but the system is still
properly working

Heating Box fan is turned on but

Air Flow reported by OKO- 3-pins – Flow Sensor” Cable is not
Properly connect the cable
TOUCH is null and the enclosure connected to Heating Box or T-Unit
temperature doesn’t increase

I check the previous Contact Okolab to receive assistance

troubleshooting but I cannot
solve the problem

47
15 Figure List

Figure 1. H201-T-UNIT-BL equipment overview. .................................................................................................................................................................7

Figure 2. Oko-touch components...................................................................................................................................................................................................8
Figure 3. H201-T-FILTER-BOX. ......................................................................................................................................................................................................9
Figure 4. DATA LOG software. .........................................................................................................................................................................................................9
Figure 5. Control Unit Overview ................................................................................................................................................................................................. 10
Figure 6. H201-T-UNIT-BL. Standard Connection.............................................................................................................................................................. 11
Figure 7.OKO-TOUCH Connection .............................................................................................................................................................................................. 12
Figure 8. CO2-O2 UNIT-BL [0-10; 1-18] and H201-T-UNIT-BL ................................................................................................................................... 13
Figure 9. Homepage of the H201-T-UNIT-BL Controller Touch Screen Display ................................................................................................. 14
Figure 10. How to change the Temperature set point (a – b) ....................................................................................................................................... 15
Figure 11. Top LED and Power Button. ................................................................................................................................................................................... 16
Figure 12. Temperature Settings (a – b) ................................................................................................................................................................................. 16
Figure 13. Temperature Settings submenu ........................................................................................................................................................................... 16
Figure 14. Chamber Mode Settings (a - b) .............................................................................................................................................................................. 17
Figure 15. Sample Mode Settings (a - b).................................................................................................................................................................................. 18
Figure 16. Free Sensor attached on the internal base of the Petri Dish ................................................................................................................... 18
Figure 17. SENSOR LID-35. For 35 mm Petri-dish ............................................................................................................................................................. 18
Figure 18. Fan speed setting (a – b). ......................................................................................................................................................................................... 19
Figure 19. Fast Heating Activation. ............................................................................................................................................................................................ 19
Figure 20. Heating Box with H201-T-FILTER-BOX ............................................................................................................................................................ 20
Figure 21. Filter Monitoring activation (a – b)..................................................................................................................................................................... 20
Figure 22. Home Page and Status Page when the usage time of the HEPA filter exceeds the maximum time of 360 days............ 21
Figure 23. Home Page and Status Page when the usage time of the Prefilter exceeds the maximum time of 180 days .................. 21
Figure 24.Warning for Prefilter replacement. ...................................................................................................................................................................... 21
Figure 25. Warning for HEPA replacement ........................................................................................................................................................................... 21
Figure 26. Air Flow Warning message when using Air Filters ..................................................................................................................................... 22
Figure 27. Status page (a – b) ....................................................................................................................................................................................................... 22
Figure 28. Cage Calibration (a-b) ................................................................................................................................................................................................ 23
Figure 29. Cage calibration warning ......................................................................................................................................................................................... 23
Figure 30. Digital input ( a- b) ...................................................................................................................................................................................................... 23
Figure 31. Touch screen settings (a- b) ................................................................................................................................................................................... 24
Figure 32. Touch Options (a – b) ................................................................................................................................................................................................ 24
Figure 33. How to enter in Brightness page .......................................................................................................................................................................... 25
Figure 34. Visual effects page (a – b) ........................................................................................................................................................................................ 25
Figure 35. Touch screen settings. Date and Time (a – b) ................................................................................................................................................ 25
Figure 36. How to enter in Alarms page (a – b). .................................................................................................................................................................. 26
Figure 37. How to set the Deviation and Time Alarm....................................................................................................................................................... 26
Figure 38. USB drive connection ................................................................................................................................................................................................. 27
Figure 39 (a) How to enter the setting menu; (b) how to enter the Touch Screen menu............................................................................... 27
Figure 40. Logging. (a) How to enter in the logging menu; (b) logging in internal memory ......................................................................... 28
Figure 41. How to access the Logging page from the Homepage ................................................................................................................................ 28
Figure 42. How to set the logging time (a – b) ..................................................................................................................................................................... 28
Figure 43. Data Logging. How to define the suffix for the downloaded files (a – b) .......................................................................................... 29
Figure 44. Downloaded files on the pen drive...................................................................................................................................................................... 29
Figure 45. Internal memory status. (a) Logging page settings; (b) Internal log page settings ..................................................................... 29
Figure 46. Download to USB ......................................................................................................................................................................................................... 30
Figure 47. Logging on USB flash drive. (a) USB drive selection; (b) Reminder to connect the USB drive .............................................. 30
Figure 48. Logging to USB drive .................................................................................................................................................................................................. 30
Figure 49. How to enter in the Overview Page (a – b)...................................................................................................................................................... 31
Figure 50. Display modalities: Icon Mode (a); Glance Mode (b). ................................................................................................................................ 31
Figure 51. Info page selection ...................................................................................................................................................................................................... 32
Figure 52. Info page OKO-Touch and H201-T-UNIT-BL (a – b). .................................................................................................................................. 32
Figure 53.HM-VF inserting in the appropriate slot............................................................................................................................................................ 34
Figure 54. How to fill the Humidity Module. ......................................................................................................................................................................... 34
Figure 55. Water Trap inserting in the appropriate slot ................................................................................................................................................. 35
Figure 56. How to empty the water trap ................................................................................................................................................................................. 35
Figure 57. Connection with the Heating Box. ....................................................................................................................................................................... 36
Figure 58. Connection with the Enclosure. ............................................................................................................................................................................ 36
Figure 59. Remove black hose and cut the tube (a – b) ................................................................................................................................................... 37
Figure 60. Cut the metal wire and hold the metal wire with scotch tape (a – b)................................................................................................. 37
Figure 61. Insert the black hose. ................................................................................................................................................................................................. 37
Figure 62. Before vs after cutting the tubes – Configuration A (a -b). ...................................................................................................................... 38
Figure 63. Before vs after cutting the tubes – Configuration B (a -b). ...................................................................................................................... 38
Figure 64. Configuration C – Configuration D (a -b). ......................................................................................................................................................... 39
Figure 65. Chamber Incubator insertion inside the enclosure..................................................................................................................................... 39
48
Figure 66. Tubing connection inside the Enclosure with HM-VF. .............................................................................................................................. 40
Figure 67. Tubing connection inside the Enclosure with HM-ACTIVE ..................................................................................................................... 40
Figure 68. Standard components on the enclosure panels. ........................................................................................................................................... 41
Figure 69. Filters Replacement .................................................................................................................................................................................................... 44
Figure 70. QR code for commercial info contact ................................................................................................................................................................. 45
Figure 71.QR code for technical info contact ........................................................................................................................................................................ 45

16 Manual Revision Table

Revision Number Additions or changes Date

01 Edited May 2018

02 Replaced some images and layout July 2018

03 Equipment correction May 2019

04 Heating tubes April 2021

IST 1254-REV 01
SV: 3.5.4.3048

49
 WARRANTY

Okolab S.r.l. warrants the “H201-T-UNIT-BL” to be free of defects in materials and workmanship for a
period of one year starting from invoice date. If the unit malfunctions, it must be returned to the factory for
evaluation. If the equipment has to be returned to the factory, please ensure that is carefully and properly packed.
Okolab S.r.l. accepts no responsibility for damage due to unsatisfactory packing. Upon examination of Okolab S.r.l.,
if the unit is found to be defective, it will be repaired or replaced at no charge. This warranty does not apply to
defects resulting from any actions of the purchaser. Components which wear are not warranted. Okolab S.r.l.
neither assumes responsibility for any omissions or errors nor assumes liability for any damages that result from
the use of its products in accordance with information provided by Okolab S.r.l. Okolab S.r.l. warrants only the
parts manufactured by it will as specified and free of defects. Okolab S.r.l. makes no other warranties or
representations of any kind whatsoever, express or implied, except that of title, and all implied warranties
including any warranty of merchantability and fitness for a particular purpose are hereby disclaimed. LIMITATION
OF LIABILITY: the total liability of Okolab S.r.l. shall not exceed the purchase price of the component upon which
liability is based. In NO event shall Okolab S.r.l. be liable for consequential, incidental or special damage.

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