Advanced® Osmometer Model 3D3

Advanced® Cryoscope Model 4D3

Service Manual

4D35SM Rev25 082714

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 Table of Contents

1. Introduction Sample Bin Setting . . . . . . . . . . . . . . .49

Specifications . . . . . . . . . . . . . . . . . . . . .7 Block Bin Setting . . . . . . . . . . . . . . . .50
General Description and Purpose . . . . .9 Stir Amplitude . . . . . . . . . . . . . . . . . .50
Service Assistance . . . . . . . . . . . . . . . . .9 Display/Printer Test . . . . . . . . . . . . . . .51
Safety/Regulatory Information . . . . . .11 Beeper Test . . . . . . . . . . . . . . . . . . . . .51
Symbol Conventions . . . . . . . . . . . . . .11 Keypad Test . . . . . . . . . . . . . . . . . . . . .51
General Cautions . . . . . . . . . . . . . . . . .11 Barcode Test . . . . . . . . . . . . . . . . . . . .51
Service & Maintenance Cautions . . . .12 Reset Serial Number . . . . . . . . . . . . . .52
Hazardous Material Cautions . . . . . . .13 Instrument Software Updates . . . . . . .53
Regulatory Notices . . . . . . . . . . . . . . .13 Performing FLASH firmware update .53
Symbol Conventions . . . . . . . . . . . . . .14 Troubleshooting Table . . . . . . . . . . . . .55

2. General Overview 5. Replacement Instructions

Major Configuration Changes . . . . . . .17 Block Probe (4D3340) . . . . . . . .4SP340
System control electronics . . . . . . . . .17 Clapper (3C2241R) . . . . . . . .3C2P241R
Motor control relays . . . . . . . . . . . . . .17 Control Board Set (3D3605/4D3605),
Operator/Supervisor keyswitch . . . . . .17 Application Board (PCB605R),
General Overview (Intel) . . . . . . . . . . .18 Processor Board (3D3620R/4D3620R)
Removing the Cover . . . . . . . . . . . . . .19 . . . . . . . . . . . . . . . . . . . . . . .4D3P620
Calibration . . . . . . . . . . . . . . . . . . . . . .20 Cooling Assembly (3D3300/4D3300)
3D3/4D3 Circuit Description (Intel) . .21 . . . . . . . . . . . . . . . . . . . . . . .4D3P300
Power Supply . . . . . . . . . . . . . . . . . . . .21 Cooling Fan (4D3360) . . . . . . . .4DP360
Front End . . . . . . . . . . . . . . . . . . . . . . .21 Display Board and Harness (4D3851)
3D3605/4D3605 Control Board Set . .21 . . . . . . . . . . . . . . . . . . . . . . .4DP851R
3D3620/4D3620 Processor Board . . . .22 EPROM (716811R) . . . . . . . . .71P811R
PCB605 Application Board . . . . . . . . .23 EPROM (716824R) . . . . . . . . .71P824R
3D3/4D3 Circuit Description (Hitachi)27 EPROM (716825R) . . . . . . . . .71P825R
Power Supply . . . . . . . . . . . . . . . . . . . .27 Ferrite Bead Rework (91035) . . .4SP475
Front End . . . . . . . . . . . . . . . . . . . . . . .27 Fuse (70011/70022) . . . . . . . . . . .700P11
3D3600/4D3600 Main Control Board .27 Head Up Relay or Head Down Relay
Design Changes . . . . . . . . . . . . . . . . . .30 (3D3109) . . . . . . . . . . . . . . . .3DP109
Replacement Parts . . . . . . . . . . . . . . . .32 Heat Transfer Fluid Pump Assembly
Supplies & Accessories . . . . . . . . . . . .32 (4D3690), Fluid Filter (4D3710),
One-way Check Valve (4D3705/
3. Maintenance 99133R) . . . . . . . . . . . . . . . . .4DP690
Cleaning and Maintenance . . . . . . . . .37 ISO 5764 Upgrade Kit (4D3101)4D3P01
Osmometer/Cryoscope Well Cleaning 39 ISO 5764 Upgrade Kit (4D3104 4D3P04
Keypad (3D3511/4D3511) . . . . .4DP511
4. Troubleshooting Load Resistor (425150R) . . . . .42P150R
Introduction . . . . . . . . . . . . . . . . . . . . .43 Mandrel (3LH500) . . . . . . . . . .3LHP500
Freeze Amplitude . . . . . . . . . . . . . . . . .44 Motor (4D3350) . . . . . . . . . . . . .4DP350
Stir Driver Noise . . . . . . . . . . . . . . . . .45 NVRAM Battery (71027R) . . . .71P27R
Head Sensor Adjustment . . . . . . . . . . .46 Power Supply Assembly (4D3950/
Head Up/Down Test . . . . . . . . . . . . . .47 325950R) . . . . . . . . . . . . . . . .325P950
Power On Diagnostics . . . . . . . . . . . . .48 Power Supply Assembly (3D3950)
A/D Tests . . . . . . . . . . . . . . . . . . . . . . .48 . . . . . . . . . . . . . . . . . . . . . . . .4DP950

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 Probe Alignment Tool Kit
(3LA700) . . . . . . . . . . . . . . . .3LP700
Sample Probe (4D3700) . . . . . . .4DP700
Sample Probe (3D3700) . . . . . . .3DP700
Sample Probe (4D3102) . . . . . . .4D3P02
Sample Probe Versions FAQ . . .4DP002
Stir/Freeze Coil (3D2404) . . . . .3DP404
Stir/Freeze Wire (3LH243/
4LH243) . . . . . . . . . . . . . . . .4LHP243
Transistor (PCB520) . . . . . . . .PCP520R
Transistor (PCB522) . . . . . . . .PCP522R
Tube Ejector Spring (3D3312/
4D3312) . . . . . . . . . . . . . . . 4DP312
Yoke (3LH230) . . . . . . . . . . . .3LHP230

6. Printer User Manual

7. Schematics
Hitachi Processor Board
PCB600 Assembly Drawing
PCB600 Main Board Schematic
Interconnection Schematic - Hitachi
Intel Processor Board
PCB605 Assembly Drawing
PCB605 Application Board Schematic
4D3605 Assembly Drawing
PCB620 Assembly Drawing
PCB620 Processor Board Schematic
Interconnection Schematic - Intel

APPENDICES
A: Symbol Definitions
B: Product Disposal and Recycling
C: Service Log

Index

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 1. Introduction

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 Model 3D3 Osmometer Specifications

Electrical: Drift: Less than 1 digit per month

Voltage: 100 to 130 VAC or 200 to 250 VAC
Ambient Temp. 1 digit for every 5°C (9°F)
Frequency: 50 or 60 Hz Effect: ambient temperature change.

Fuses: 2-Amp time delay for 100-130 VAC; Start-up Time: From stand-by: immediately
1-Amp time delay for 200-250 VAC From power on: under 5 minutes

Power: 150 Watts Test Time: Approximately 2 minutes per sample

in 0 to 2000 mOsm range; moderately
Memory Backup: integral lithium cell; longer in high range.
10 years typical
Dimensions inches centimeters
Sample Volume: 0.20 or 0.25 mL Width: 12.5 32
Depth: 16.5 42
Sample Capacity: Single sample Height: 11.5 29
With Head Up: 16 41
Readout: 20-character digital display
Weight pounds kilograms
Units: mOsm/kg H2O Net: 27 12
Shipping: 38 18
Range:
Low: 0 to 2000 mOsm/kg H2O Warranty: One-year limited warranty on work-
High: 1400 to 4000 mOsm/kg H2O manship and all parts except glass,
plastic and parts warranted by their
Linearity: Less than ±0.5% from a straight line makers.

Resolution: 1 mOsm/kg H2O

Certification:
Repeatability:
0 to 400 mOsm ±2 mOsm/kg (1 S.D.)
400 to 4000 mOsm ±0.5% (1 S.D.)
Refer to Regulatory Notices (see page 11) for appli-
Communications: cable standards.
DTE RS-232C, parallel printer, barcode

Storage Temperature: - 40°C to +45°C

(- 40°F to +113°F)

Operating Temperature
(ambient): 18°C to 35°C
(64°F to 95°F)

Room Humidity: 5 to 80% R.H.

(non-condensing)

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 Model 4D3 Cryoscope Specifications

Electrical: Start-up Time: From stand-by: immediately

Voltage: 100 to 130 VAC or 200 to 250 VAC From power on: under 5 minutes

Frequency: 50 or 60 Hz Test Time: Approximately 90 seconds in

30-second timed mode
Fuses: 2-Amp time delay for 100-130 VAC;
1-Amp time delay for 200-250 VAC Dimensions inches centimeters
Width: 12.5 32
Power: 150 Watts Depth: 16.5 42
Height: 16 41
Memory Backup: integral lithium cell;
10 years typical Weight pounds kilograms
Net: 27 12
Sample Volume: 2.0 to 2.5 mL Shipping: 38 18

Sample Capacity: Single sample Warranty: One-year limited warranty on work-

manship and all parts except glass,
Readout: 20-character digital display plastic and parts warranted by their
makers.
Units: -m°C or -m°H

Range: 0 to -1000 m°C or m°H Certification:

Linearity: Typically ±0.5% from a straight line

Resolution: ±1 m°C or m°H Refer to Regulatory Notices (see page 11) for appli-
cable standards.
Repeatability: ±2 m°C or m°H (1 S.D.)

Communications:
DTE RS-232C, parallel printer, barcode

Storage Temperature: - 40°C to +45°C

(- 40°F to +113°F)

Operating Temperature
(ambient): 18°C to 35°C
(64°F to 95°F)

Room Humidity: 5 to 80% R.H.

(non-condensing)

Drift: Less than 1 digit per month

Ambient Temp. 1 digit for every 5°C (9°F)

Effect: ambient temperature change.

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 General Description and Purpose Service Assistance

Advanced® Osmometers and Cryoscopes To contact Advanced Instruments Product

determine the concentration of solutions Service:
through freezing-point depression measure- • Call (800) 225-4034 (toll-free within the
ment. Osmometers are used primarily to USA and Canada; after normal business
measure body fluids such as serum and urine, hours, dial extension 2191)
and cryoscopes are used to determine the
freezing points of various liquids and to • Call +US (781) 320-9000 (elsewhere)
detect added water in milk. Application infor- • Or fax (781) 320-3669
mation is available upon request.
When calling Advanced Instruments, have the
Except for different cooling assemblies, model and serial number from the label on the
stir/freeze wires, sample sizes and software, back of the instrument, and a description of
the 3D3 and the 4D3 are identical to one the problem. Use a telephone as close to the
another. For clarity when replacing parts or instrument as possible in order to facilitate
troubleshooting, the instructions indicate any making recommended diagnostic checks.
significant differences between the two instru-
ments. A service technician may assist in making
minor repairs over the phone, providing you
The Service Manual with recommended parts (or part numbers), or
may issue an authorization (RMA) to ship the
This Service Manual contains: instrument for factory repair.

• instructions for performing repairs. The RMA procedure is as follows:

• guides for troubleshooting operational and 1. Contact Advanced Instruments to obtain
mechanical problems. an RMA.
• circuit system illustrations. 2. Pack and ship the instrument in its origi-
• information about replacement parts and nal carton. Do not send instrument sup-
service repairs. ply items.

This manual is designed to assist 3. Prepay shipment to the factory. Advanced

service technicians, and does not Instruments cannot accept collect ship-
imply a license to perform repairs ments. Insure the shipment or accept the
without proper qualifications. damage risk.

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 Notes:

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 Safety / Regulatory Information
To reduce the risk of bodily injury, This symbol indicates the presence
electric shock, fire, and damage to of a fuse.
your instrument, please read and
observe the following precautions.
This symbol indicates the presence
• If the product is used in a manner not in of protective earth ground.
accordance with the equipment design,
operating instructions or manufacturer's
recommendations, the operation of the This symbol indicates the power is
product may be impaired to the extent that ON.
a safety hazard is created.
• Do not attempt to perform electrical work This symbol indicates the power is
if you are not fully qualified. This manual OFF.
is not a substitute for electrical training.
General Cautions
Symbol Conventions
• This product should be operated only with
The exclamation point within an the type of power source indicated on the
equilateral triangle is intended to product’s electrical ratings label. Refer to
alert the user to the presence of the installation instructions included with
important operating and maintenance the product.
(servicing) instructions in the litera-
• If the power cord provided is replaced for
ture accompanying this product.
any reason or if an alternate cord is used,
the cord must be approved for use in the
The lightning flash with arrowhead
local country. The power cord must be
symbol within an equilateral triangle
approved for the product’s listed operating
is intended to alert the user to the
voltage and be rated at least 20% greater
presence of uninsulated dangerous
than the ampere ratings marked on the
voltage within the product's enclo-
product’s electrical ratings label. The cord
sure that may be of sufficient magni-
end that connects to the product must have
tude to constitute risk of electric
an IEC 60320 connector.
shock to persons.
• Plug the product into an approved ground-
The static symbol within an equilat- ed electrical outlet.
eral triangle is intended to alert the
• Do not disable the power cord’s grounding
user to the presence of internal com-
plug.
ponents that could be damaged by
static electricity. • If an extension cord or power strip is used,
make sure that the cord or strip is rated for
This static symbol is intended to the product, and that the total ampere rat-
alert the user to the presence of a ings of all products plugged into the exten-
specific component that could be sion cord or strip do not exceed 80% of the
damaged by static electricity. cord’s or strip’s rating limit.

This symbol indicates the presence • Route power cords so that they will not be
of alternating current (AC). walked on, tripped on, or pinched by items
placed upon or against them. Pay particu-

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 lar attention to the plug, electrical outlet, - Liquid has been spilled into the interior
and the point where the cord exits the of the product.
product.
- A foreign object has fallen into the
• Do not pull on cords and cables. When product.
unplugging cords or cables, grasp the cor-
- The product has been dropped or dam-
responding connector.
aged by a falling object.
• Do not install or use this product in any
- There are noticeable signs of overheat-
area subject to extreme short-term temper-
ing or a burning odor.
ature variations, or locations that exceed
the specified operating environment tem- - The product does not operate normally
peratures. when you follow the operating proce-
dures.
• Never use this product in a wet area.
- The main supply fuse(s) or any internal
• To avoid injury or fire hazard, do not oper-
fuse(s) continually fail.
ate this product in an explosive atmos-
phere. • A discharge of static electricity from con-
tact with the human body or other conduc-
• Do not install or use the product on an
tor may damage system boards or static
unstable, non-level work surface.
sensitive devices. Never perform internal
• Do not operate this product with the covers maintenance without following recom-
removed or unsecured. mended static protection procedures.
• The product is equipped with operator
Service & Maintenance Cautions
accessible fuses. If a fuse blows, it may be
due to a power surge or failure of a com-
• Unplug the power cord prior to opening or
ponent. Replace the fuse only once. If the
removing covers, or else you may be
fuse blows a second time, it is probably
exposed to electric shock, excessive tem-
caused by failure of a component part. If
peratures, or mechanical hazards.
this occurs, refer service to qualified serv-
• Performing service or maintenance not ice personnel. Always replace the fuse
detailed in the User’s Guide, with or with- with one of the same rating, voltage, and
out this Service Manual, should only be type. Never replace the fuse with one of a
done by a qualified service technician. higher current rating.
• Never restrict airflow into or out of the • When servicing the product, use only
product. Occasionally, check the air vents factory-specified parts.
for blockage.
• WARNING: When returning this product
• Wipe the exterior of the product with a for service, or shipping this product to a
soft, damp cloth as needed. Using cleaning second location, remove all hazardous
products other than those specified, may specimens and decontaminate the product
discolor or damage the finish. before packaging for shipment. If the
product cannot be decontaminated, consult
• If the product requires service for any of with your shipping agent on appropriate
the following reasons, unplug the product packaging and marking.
from the electrical outlet and refer service
to a qualified service technician.
- The power cord, extension cord, power
strip or power input module is damaged.

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 Regulatory
Hazardous Material Cautions approval type Description

• WARNING: Handle all biohazardous mate- U.S. Safety This product has been listed by
rials according to established good laborato- ETL testing laboratories as being
ry practices and follow your institution’s in compliance with the require-
exposure control plan. Persons handling ments of UL 3101-1, 1st Edition,
human blood and body fluid samples must "Electrical Equipment for
Laboratory Use; Part 1: General
be trained in blood-borne hazards and
Requirements". The "US" in the
observe universal precautions. Universal pre- lower right of the ETL mark
cautions is an approach to infection control, demonstrates this listing.
where all human blood and body fluids are
treated as if known to be infectious. Use per- Canadian Safety This product has been listed by
sonal protective equipment such as gloves, ETL testing laboratories as being
in compliance with the require-
gowns, etc., to prevent exposure. Store bio-
ments of CAN/CSA C22.2
hazardous materials in regulated waste con- No.1010.1-92, "Safety
tainers and dispose of these materials in a Requirements for Electrical
safe and acceptable manner that is in com- Equipment for Measurement,
pliance with all country, state and local Control and Laboratory Use;
requirements. General Requirements";
Including Amendment Two. The
• If a biohazardous material is spilled on or "C" in the lower left of the ETL
inside the equipment, decontaminate the mark demonstrates this listing.
equipment using a 1% bleach solution, or as
EC Declaration of This product meets the intent of
outlined by those policies and procedures Conformity - EMC Directive 89/336/EEC for
established within your institution. Electromagnetic Compatibility.
Compliance was demonstrated
• To avoid injury or fire hazard, do not operate using the following standards, as
this product in an explosive atmosphere. listed in the Official Journal of
the European Communities:
Consult the Declaration of Con-
Regulatory Notices formance certificate shipped with
the product for the latest update.
• This product has been designed and manu- • EN 61326: 1998, Group 1,
Class B, "Electrical Equipment
factured in accordance with U.S., Canadian,
for Measurement, Control, and
and European regulatory requirements as Laboratory Use".
outlined below. Modifications made to this
product that are not expressly approved in EC Declaration of: This product meets the intent of
writing by the manufacturer will void the Conformity - Low Directive 73/23/EEC, the Low
user’s authority to operate this product, pre- Voltage Voltage Directive. Compliance
was demonstrated using the fol-
viously issued factory approvals, and the
lowing standards as listed in the
user’s rights under the warranty. Official Journal of the European
Communities: Consult the
• The distributor or dealer may have applied
Declaration of Conformance cer-
additional local, national, or international tificate shipped with the product
approvals to this product. Consult the dis- (if required) for the latest update.
tributor or dealer for more information and · EN 61010-1 (1993), "Safety
documentation. Requirements for Electrical
Equipment for Measurement,
• Connections to this product must be made Control and Laboratory Use -
with shielded cables. Use of non-shielded General Requirements";
cables may violate RFI/EMI limits. Including Amendment Two
(1995).

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 Regulatory
approval type Description

EC Declaration of This product meets the intent of

Conformity - WEEE Directive 2002/96/EC as amended
by 2003/108/EC for Waste
Electrical and Electronic
Equipment (WEEE). Consult the
Declaration of Conformance cer-
tificate shipped with the product
(if required) for the latest update.

Symbol Conventions

This symbol indicates conformity to

relevant European directives.

This symbol indicates the product

was tested to conform to relevant
Canadian and U.S. safety standards
by Intertek Testing Services NA,
Inc. The ETL mark is approved in
the United States as a Nationally
Recognized Testing Lab (NRTL) by
OSHA, and in Canada by the
Standards Council of Canada.

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 2. General Overview

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 Major Configuration Changes

Over the years, Advanced Instruments, Inc. The larger board is called the application
has made several improvements to the board, and the smaller board is called the
3D3/4D3 system. It is important to identify processor board. The processor board
some of these changes to properly service contains an Intel 80C186 microprocessor
your instrument. Although every attempt has and two field-programmable Flash
been made to cover all the configurations, EPROM's. All references to this configu-
some descriptions may vary from what is ration will use the terms control board
present in your particular situation. If you are set, application board, or processor
having difficulty comparing your system to board.
this manual, consult the factory for assistance.
Please refer to the applicable system photo- Motor control relays
graphs and circuit descriptions once you have
1. The original configuration used two chas-
identified your configuration. You may also
sis-mounted relays located on the center
refer to suffix history chart located in the
rear interior wall.
Design Changes section of this manual, but
keep in mind that many of these changes were 2. The second configuration used two PCB-
backward-compatible, so use the chart only as mounted relays located on the center rear
a reference. interior wall. This version may be used
as a replacement for the first configura-
tion.
System control electronics
1. The original main control board was a
Operator/Supervisor keyswitch
single board located on the right side,
containing a Hitachi 63A03 microproces- 1. The first configuration had no keyswitch.
sor and a 256K Byte EPROM. This All references to keyswitch position in
board can be identified by the heat sink this manual should be ignored.
mounted component in the upper left cor-
ner of the main control board. This board 2. The second configuration used a black
and firmware are no longer supported, as plastic keyswitch located on the rear of
of October 1, 2001. the instrument. This keyswitch, when in
the operator position, locked the user out
2. The second main control board was a sin- of these features: setup, diagnostic test
gle board located on the right side, also utilities, and calibration.
containing a Hitachi 63A03 microproces-
sor, but had a 512K Byte EPROM. This 3. The third and current configuration uses a
board can be identified by the lack of a metal keyswitch located on the rear of the
heat sink mounted component in the instrument. This keyswitch, when in the
upper left corner of the main control operator position, locks the user out of
board. This board and firmware are no these features: setup and calibration.
longer supported, but can be replaced Note: If you have configuration number 2
with the Intel configuration. All refer- (above) and you replace the main con-
ences to this configuration will use the trol board with the control board set,
term main control board. the operator and supervisor positions
3. The third and current control board, locat- will be reversed, and your keyswitch
ed on the right side, is a two-board set. will now only lock out the setup and
calibration features.

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 General Overview
(Intel Processor Systems)

L M N O
K P Q R

I
H
G
F

S
D T

C
U

B A

A. Head L. Sample Probe Plugin

B. Motor M. Block Probe Plugin
C. Driver Transistors (Stir, Thermoelectric) N. Main Board Set (Application/Processor)
D. Motor Capacitor O. Power Supply
E. Head Up/Head Down Relays P. Keypad Transition Board
F. Power Entry Module Q. Keypad
G. RS-232C Port R. Display
H. Printer Port S. Deck
I. Printer Power Outlet T. Heat Transfer Return Tube
J. Supervisor/Operator Keyswitch U. Heat Transfer Inlet Tube
K. Barcode Port

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 Removing the Instrument Cover & the Head Cover

Tools Needed: Flat-bladed screwdriver, The following instructions refer to 4D3

Phillips screwdriver. cryoscopes without a suffix:

1. Turn off the power and unplug the instru-

Warning-Hazardous Voltage ment. Remove the heat transfer fluid
from the front cover.
Warning-Internal components may
2. Remove the two screws from the rear
be damaged by static electricity.
panel of the back cover (B) and lift off
the back cover.
CAUTION: Improper connections
may cause damage to the instrument. 3. Remove the four screws from underneath
the front cover, lift the front cover slightly
up and then slide it forward and off.
The following instructions refer to 4D3
cryoscopes with suffix "A" or later, and to 4. Remove the keypad display connectors
all 3D3 osmometers: from the keypad on the front cover.
5. Remove the two screws from the head
1. Turn off the power and unplug the instru- cover (C) and lift off the head cover.
ment.
2. Remove the two sets of screws from the
side panels (A) and the two screws from
the rear panel (B).
3. Slide the instrument cover up and off.
4. Remove the two screws from the head
cover (C) and lift off the head cover.

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 Calibration

The 3D3 has two ranges, each with its own

calibration. To calibrate the low range, three Calibrate the instrument according to the
freezing-point tests are required at the 100 instructions below:
mOsm/kg level, and six tests are required at
the 1500 mOsm/kg level. To calibrate the 1. Turn the instrument on.
high range, six freezing-point tests are
required at both the 1500 mOsm/kg level and 2. If there is a Supervisor/Operator key-
the 3000 mOsm/kg level. switch, turn it to the Supervisor position.
It must remain there throughout calibra-
The 4D3 allows timed or plateau-seeking cali- tion.
bration in either the Hortvet (m°H) or the
Celsius (m°C) scale. Six freezing-point tests 3. Press CALIB to begin the calibration
are required at each of the 4D3s two calibra- sequence.
tion levels:
4. The display will prompt you at each cali-
-408 m°C (-422 m°H), and
bration level. Place a sample of the first
-600 m°C (-621 m°H).
calibration standard into the freezing
chamber and press START.
Note: On instruments using the Intel proces-
sor and control board set, your cali-
5. Continue loading and testing samples at
bration may vary slightly from that
the first calibration level until the display
mentioned here. If you see the mes-
prompts you for samples at the second
sage “Recalibration Needed”, follow
calibration level.
the instrument prompts to complete the
initial calibration.
6. Place the sample of the second calibration
standard into the freezing chamber and
press START.

7. When the instrument has completed cali-

bration, the display will read
"Calibration Complete".

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 3D3/4D3 Circuit Description
(Intel Processor)
The 3D3/4D3 is comprised of the following (whose duty cycle is controlled through the
functional blocks: block probe/thermistor) supercool the sample.
The stir/freeze wire constantly stirs the sam-
ple to guarantee uniform cooling. When the
Power Supply
supercooled sample reaches a set temperature
(3000 mOsm for the 3D3 in the low range
The power entry assembly interfaces the AC
and 5400 mOsm for the 3D3 in the high
voltage presented to the instrument with the
range; 3000 m°C for the 4D3), the stir/freeze
transformer. The transformer provides 120
wire "buzzes" causing the sample to freeze.
VAC to the power supply board and the head
During a test, the fan is on while the thermo-
motor. The power supply provides the power
electrics duty cycle is greater than 50%; oth-
to run the main printed circuit board (±12
erwise the fan is off. In between tests, the fan
VDC, ±5 VDC), the thermoelectrics (5 VDC),
runs as required to maintain the software con-
the fan (12 VDC), the stir/freeze coil
trolled target temperatures.
(12 VDC), and the optional external printer
(12 VDC).
3D3605/4D3605 Control Board Set
Description
Front End
The 3D3 and 4D3 use the same control board
Although the 3D3 and 4D3 have physically
set except for the processor board configura-
different front ends, the following description
tion.
applies to both.
General: The control board set is made up of
The sample thermistor measures the tempera-
two printed circuit boards in a motherboard/
ture of the sample. The thermoelectrics
daughterboard configuration.

Processor
Board

Barcode Power
Port Supply

Applications Cooling
Serial Port
Board System

Head Motor Printer

Display Keypad
Controls Port

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 3D3620/4D3620 Processor Board Input/Output Map: The 64 kilo-bytes of
I/O address space control access to all periph-
Processor: The processor used is an Intel erals such as the serial ports, application
80C186 16-bit embedded processor. The board, etc. The processor uses 256 bytes,
80C186 contains three programmable 16-bit called the Peripheral Control Block, to control
timers, two serial ports, programmable inter- the integrated serial ports, timers, interrupts,
rupts, 1 mega-byte of memory address space, chip selects, and I/O pins. The Peripheral
and 64 kilo-bytes of input/output (I/O) Control Block is fully described in the
address space. The processor uses an external 80C186EB/80C188EB Microprocessor User’s
32 MHz crystal to generate the internal 16 Manual. The application board’s registers and
MHz system clock. The processor controls the CPU board’s registers control access to
access to all memory and all I/O. various functions and are described later.

Memory Map: The 1 mega-byte of memory Memory: Two types of memory are avail-
address space contains read-only memory able: read-only and read/write. The chips
(Flash EPROM), read/write memory (static used here have 8-bit wide data buses. Used in
RAM), and the real time clock. About half of pairs, the memory becomes compatible with
the address space is unused, allowing for the processor’s 16-bit wide data bus. The
future expansion. The read-only memory is read-only memory is implemented using two
divided into four sections: reset vector, boot 128 kilo-bytes x 8 bits Flash EPROMs. Flash
code, parameter blocks (unused), and appli- memory is only programmable when a pro-
caiton code. The reset vector tells the proces- gramming voltage (in this case, 12 volts DC)
sor where to first start executing code; in this is applied to the memory; otherwise, it
case, the boot code is executed first after behaves like read-only memory. The boot
reset. The boot code determines if new soft- code controls the programming voltage, since
ware will be downloaded by checking if the this is the only place where new software can
dip switch is in the “PROGRAM” position. be downloaded. The read/write memory is
If it is, the boot code waits for new software implemented using two 128 kilo-bytes x 8
to be downloaded through the serial port. If bits static RAMs.
not, the application code starts.
Watchdog Timer: The watchdog timer pro-
The real time clock maintains the current date vides a mechanism to reset the processor
and time, and contains nonvolatile memory when the software is not behaving normally.
where the instrument’s operation parameters The software “pets” the watchdog every 100 -
are stored. A internal lithium battery pre- 200 milliseconds. As long as the watchdog is
serves the contents of the nonvolatile memory “petted”, nothing happens. However, if the
when the instrument is turned off. Finally, the sotware stops “petting” the watchdog, the
read-write memory is used for stacks, vari- watchdog “bites” the processor by activating
ables, etc., except for the small section allo- the processor’s RESET pin, causing the
cated for the interrupt vectors. The interrupt processor to reset. The watchdog will also
vectors tell the processor what code to run reset the processor if the power supply falls
when an interrupt occurs, and functions simi- below 4.75 VDC. These two features allow
larly to the reset vector. the instrument to recover from abnormal soft-
ware and hardware conditions.
Please note that all memory addresses and
sizes are always defined in terms of bytes, Real Time Clock (RTC): The real time
even though the processor handles words (1 clock maintains the current date and time.
word = 2 bytes, 1 byte = 8 bits; therefore, 16 The RTC uses an internal clock circuit with
bits = 1 word). an internal, 10-year life, lithium battery to

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 perform timekeeping. The battery also pre- kilo-ohms at -12ºC. Typically, a 0.6 ohm
serves the contents of internal memory. change in the thermistor’s resistance equates
to a 1 mOsm/1.86mºC change. A separate
Dip Switch: The three-position dip switch Wheatstone bridge circuit is used to measure
allows the user to download new software via the voltage across each thermistor probe.
one switch. The other two switches are avail- This voltage is first filtered and then sampled
able for selecting different operating modes. by the analog-to-digital converter. The ana-
These operating modes and the process for log-to-digital converter uses a sigma-delta
downloading software are described later in conversion technique with on-chip filtering
this manual. and a 6.25 VDC reference voltage.

Voltages: Three voltages are present on the Drive Circuitry: Drive circuitry is provided
CPU board: +5 VDC, +12 VDC, and VPP. to turn on and off the four high current loads
+5 VDC powers all the logic. +12 VDC is such as the stir wire, the head motor, the ther-
switched on and off to create the flash memo- moelectric cooler, and the fan.
ry’s programming voltage, VPP.
The stir wire coil is controlled by the rear
Glue Logic: The glue logic performs the panel-mounted darlington transistor (Q2B).
functions of creating the RAM memory chip The drive circuit consists of a programmable
selects, the Flash memory write signals, the timer, D/A converter, and interface op-amps.
VPP control signal, the watchdog control sig- The timer provides a square wave of approxi-
nal, and accessing the dip switch. The CPU mately 71Hz, while the D/A converter con-
board has two software accessible hardware trols the output amplitude to the darlington
registers to read the dip switch and to control transistor.
watchdog and VPP.
The head motor is controlled by two relays
Connectors: A 64-pin connector provides located at the rear of the chassis that interface
address, data and interrupt signals. A 16-pin the 120 VAC motor to the DC logic. The
connector provides general chip selects and upper relay raises the head, while the lower
serial receive/transmit signals. The applica- relay lowers the head. The relays get their
tion PCB uses a subset of the available sig- commands from the application board via two
nals. FETs and the application logic. LEDs are
provided on the application board for moni-
toring the drive signals. LEDs are also pro-
PCB605 Application Board vided to monitor the signals from the head
sensor board, described below.
The application PCB contains all circuitry
specific to the 3D3/4D3 instruments. The The thermoelectric cooler is controlled by the
board includes voltage supplies, indicators, FET driver transistor mounted on the rear
analog-to-digital converters, drive circuitry, panel (Q1B). The FET is, in turn, controlled
parallel ports, serial ports, and various switch- by the microprocessor through the PLD appli-
es. cation logic. The processor varies the duty
cycle square wave in response to software
Analog-to-Digital Converters: Two analog- commands and block probe resistance.
to-digital converters are provided; one for the
sample thermistor probe, and one for the The fan is controlled by a FET transistor on
block thermistor probe. The thermistors vary the application board, that is interfaced to the
in resistance from approximately 2 kilo-ohms processor through the application logic.
at room temperature, to approximately 10

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 I/O Ports: Circuitry for the two serial ports Pin 1 (left) +12 VDC
(RS-232 and barcode) and the parallel printer Pin 2 (center) not connected
port, is present on the application board with Pin 3 (right) ground
cabling to the actual connectors on the back
panel. Printer Port: The printer port connector on
the back of the instrument provides 8 data
Display & Keypad: The display and keypad lines and the necessary handshaking signals
are interfaced to the processor via program- for communication with most standard print-
mable logic devices (PLDs). The keypad is ers. The printer port connections are as fol-
polled in a row/column process which identi- lows:
fies the key pressed to the microprocessor.
The application logic, in response to proces- Pin-Out
sor commands, controls the LEDs located on
Signal Port Connector Cable Output
the keypad.
Strobe 8 1
Data 0 2 2
Light Emitting Diodes (LED): LEDs are
Data 1 3 3
provided to indicate when a high current load
Data 2 4 4
is active, a sensor is tripped, or a voltage sup-
Data 3 5 5
ply is active. Green LEDs are used on the
Data 4 6 6
power supply voltages, yellow LEDs are used
Data 5 14 7
on driver signals such as the fan and head
Data 6 7 8
motor controls, and red LEDs are used to
Data 7 15 9
indicate that the head up and down optical
Ready 1 11
sensors have been tripped.
Ret. Gnd 9 19
Ret. Gnd 10 20
Application Logic: The application logic is
Ret. Gnd 11 21
made up mainly of two programmable logic
Ret. Gnd 12 22
devices (PLDs). These provide software
Ret. Gnd 13 23
accessible hardware registers, enabling the
software to read the keypad, sensors, and ana-
Serial (RS-232) Port Interface: An RS-232
log-to-digital converters. These also allow
line driver/receiver provides the microproces-
manipulation of the display, external compo-
sor with a serial port interface that supports
nents such as the fan, head motor, etc., and
both hardware and software handshaking.
interface to the RS-232 ports. The printer is
controlled directly from the microprocessor
The DB-9 RS-232 port conforms to the DTE
through an 8-pin D-latch.
RS-232C standard and has the following pin
assignments:
Connectors: Connectors are supplied for the
CPU PCB, display, keypad, power, drives,
Signal Pin Direction
probes, and back panel I/O ports.
Carrier Detect 1 to 3D3/4D3
Receive Data 2 to 3D3/4D3
Printer Power Outlet: A printer power out-
Transmit Data 3 from 3D3/4D3
let eliminates the need for a wall transformer
Data Terminal Ready 4 from 3D3/4D3
with an external printer. The 3-pin DIN sock-
Signal Ground 5 common
et, located on the back of the instrument, sup-
Data Set Ready 6 to 3D3/4D3
plies up to 1 Amp at 12 VDC via the follow-
Request to Send 7 from 3D3/4D3
ing connections:
Clear to Send 8 to 3D3/4D3

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 Note that your instrument is only designed to Signal Pin Direction
support unidirectional communication with an +5V DC 1 to reader
external device. At this time, there is no pro- receive data 10 from reader
tocol for bidirectional communication. gnd/earth 9 common
For a sample RS-232 Port Setup, please see Barcode Port Connections
the RS-232 Supplemental Information in the
Appendix at the end of this user’s guide. A suitable barcode scanner is available from
Advanced Instruments. To interface with the
Note: This instrument requires the use of a 4D3, the barcode scanner must be pro-
null modem RS-232C cable. There are grammed as follows, referring to the scanner
several variations on null modem users guide.
cables. Advanced Instruments recom-
1200 bps
mends that you purchase a RS-232C
CR suffix
cable direct from our factory.
disable beep after good decode
triggerless trigger mode (optional)
Supervisor/Operator Keyswitch Interface:
A PLD is used to interface the supervisor/
operator keyswitch to the microprocessor. In
Operator position, the user cannot change
* 4D3 cryoscopes that have a suffix before
setup or calibration settings.
"E" and 3D3 osmometers that have a suf-
fix before "C" do not have the head tran-
Head Transition Board (M20450) Descrip-
sition board.
tion: The stir/freeze coil and the sample
** 4D3 cryoscopes without a suffix do not
probe are connected to the head transition
have the head sensor board or the keypad
board which allows easy sample probe
transition board.
replacement.*

Head Sensor Board (3D3380) Description:

This board provides two optical sensors that
interface to a PLD on the main board. These
two sensors detect the head-up and head-
down conditions.**

Keypad Transition Board (FLO520)

Description: This board provides the inter-
face to the keypad along with pull-up resistors
for the keypad LEDs.**

Barcode Port: A D-type, 15-pin barcode port

is provided in the back of the 4D3 for con-
necting and providing power to such a device.
For proper operation, the barcode port
requires a 1200 bps, RS-232 signal providing
asynchronous serial data containing 1 start
bit, 8 data bits, 1 stop bit, and no parity.

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 Notes:

The Advanced® 3D3/4D3 Service Manual 26

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 3D3/4D3 Circuit Description
(Hitachi Processor)
The 3D3/4D3 is comprised of the following The sample thermistor measures the tempera-
functional blocks: ture of the sample. The thermoelectrics
(whose duty cycle is controlled through the
block probe/thermistor) supercool the sample.
Power Supply
The stir/freeze wire constantly stirs the sam-
ple to guarantee uniform cooling. When the
The power entry assembly interfaces the AC
supercooled sample reaches a set temperature
voltage presented to the instrument with the
(3000 mOsm for the 3D3 in the low range
transformer. The transformer provides 120
and 5400 mOsm for the 3D3 in the high
VAC to the power supply board and the head
range; 3000 m°C for the 4D3), the stir/freeze
motor. The power supply provides the power
wire "buzzes" causing the sample to freeze.
to run the main printed circuit board (±12
During a test, the fan is on while the thermo-
VDC, ±5 VDC), the thermoelectrics (5 VDC),
electrics duty cycle is greater than 50%; oth-
the fan (12 VDC), the stir/freeze coil
erwise the fan is off. In between tests, the fan
(12 VDC), and the optional external printer
runs on slow speed to provide cooling for the
(12 VDC).
electronics.
Front End
3D3600/4D3600 Main Control Board
Description
Although the 3D3 and 4D3 have physically
different front ends, the following description
The 3D3 and 4D3 use the same main board
applies to both.
except for the EPROM (which contains the
software).

Power Supply

Electronics Cooling
Serial Port
Package System

Head Motor Printer

Display Keypad
Controls Port

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 General: The microprocessor is an 8 bit through the keypad transition board (see
Hitachi 63A03R (a member of the 6800 fami- below), to the microprocessor. A 3 to 8 line
ly of microprocessors). The microprocessor decoder is used to pulse the different columns
runs at approximately 1 MHz and has an of the keypad to check for key presses. The
address space of 64 kBytes. The micro- microprocessor controls the keypad LEDs.
processor runs the software stored in
48 kByte of EPROM and uses the battery- Display and Port Interfaces: A peripheral
backed-up 8 kByte static RAM to store infor- interface adapter is used to interface both the
mation. display and the printer port to the micro-
processor. A printer power outlet (see below)
A/D Converters: Two dual-slope A/D con- eliminates the need for a wall transformer
verters using programmable timer modules with an external printer.
provide interface from the sample and block
probes to the microprocessor. The A/D con- The printer port connector on the back of the
verters use a reference voltage of 6.25 VDC instrument provides 8 data lines and the nec-
and provide 15 bits (1/4 ohm) of resolution. essary handshaking signals for communica-
tion with most standard printers. The printer
Front End Interface: The thermoelectrics port connections are as follows:
are interfaced to a programmable timer mod-
ule through discrete logic. The programmable Pin-Out
timer module interfaces directly to the micro-
Signal Port Connector Cable Output
processor. The stir/freeze wire is interfaced to
Strobe 8 1
the D/A converter through discrete logic.
Data 0 2 2
The power transistors, mounted either on the
Data 1 3 3
back of the instrument or underneath the
Data 2 4 4
power supply, control the thermoelectrics and
Data 3 5 5
the stir/freeze wire. The fan is interfaced to
Data 4 6 6
the microprocessor through discrete logic.
Data 5 14 7
Data 6 7 8
D/A Converter: The D/A converter uses a
Data 7 15 9
60 Hz 0-5 volt reference from a programma-
Ready 1 11
ble timer module through discrete logic. The
Ret. Gnd 9 19
D/A converter interfaces directly to the micro-
Ret. Gnd 10 20
processor.
Ret. Gnd 11 21
Ret. Gnd 12 22
Head Motor Interface: The AC motor run-
Ret. Gnd 13 23
ning at 120 VAC controls the head (which
contains the sample probe and the stir/freeze
Serial (RS-232) Port Interface: An asyn-
wire). Two relays mounted to the back of the
chronous Communications Interface Adapter
instrument control the voltage to the AC
and RS-232 line drivers/receivers provide the
motor. These relays interface to a peripheral
microprocessor with a serial port interface
interface adapter through discrete logic. The
that supports both hardware and software
peripheral interface adapter interfaces directly
handshaking.
to the microprocessor. Two optical sensors
(see the head sensor board below) detect the
The DB-9 RS-232 port conforms to the DTE
head up and head down positions.
RS-232C standard and has the following pin
assignments:
Keypad Interface: A peripheral interface
adapter is used to interface the keypad,

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 Signal Pin Direction Printer Power Outlet
Carrier Detect 1 to 3D3/4D3 The 3-pin DIN socket, located on the back of
Receive Data 2 to 3D3/4D3 the instrument, supplies up to 1 Amp at 12
Transmit Data 3 from 3D3/4D3 VDC via the following connections:
Data Terminal Ready 4 from 3D3/4D3
Signal Ground 5 common Pin 1 (left) +12 VDC
Data Set Ready 6 to 3D3/4D3 Pin 2 (center) not connected
Request to Send 7 from 3D3/4D3 Pin 3 (right) ground
Clear to Send 8 to 3D3/4D3

Note that your instrument is only designed to * 4D3 cryoscopes that have a suffix before
support unidirectional communication with an "E" and 3D3 osmometers that have a suf-
external device. At this time, there is no pro- fix before "C" do not have the head tran-
tocol for bidirectional communication. sition board.
** 4D3 cryoscopes without a suffix do not
For a sample RS-232 Port Setup, please see have the head sensor board or the keypad
the RS-232 Supplemental Information in the transition board.
Appendix at the end of this user’s guide.

Note: This instrument requires the use of a

null modem RS-232C cable. There are
several variations on null modem
cables. Advanced Instruments recom-
mends that you purchase a RS-232C
cable direct from our factory.

Supervisor/Operator Keyswitch Interface:

A peripheral interface adapter is used to inter-
face the supervisor/operator keyswitch to the
microprocessor.

Head Transition Board (M20450) Descrip-

tion: The stir/freeze coil and the sample
probe are connected to the head transition
board which allows easy sample probe
replacement.*

Head Sensor Board (3D3380) Description:

This board provides two optical sensors that
interface to a peripheral interface adapter on
the main board. These two sensors detect the
head-up and head-down conditions.**

Keypad Transition Board (FLO520)

Description: This board provides the inter-
face to the keypad along with pull-up resistors
for the keypad LEDs.**

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 Design Changes

The serial number suffix (referred in this

manual simply as the suffix) indicates the
revision of the instrument. The charts below
list the major changes made at each revision
of the 3D3 Osmometer and the 4D3
Cryoscope.

Model 3D3 Model 3D3

Osmometer Description Osmometer Description

No suffix Original model released. Serial Suffix ‘N’ • Software change.

April 1993 November 1997 • Low/High range correction.

Serial Suffix ‘A’ CSA certification changes. Serial Suffix ‘O’ ---
July 1993 omitted

Serial Suffix ‘B’ Changes to accommodate sharing Serial Suffix ‘P’ Correct reboot problem.
October 1993 parts with Mark 2. January 1998

Serial Suffix ‘C’ • Software for multi-language Serial Suffix ‘Q’ 4-digit year display, for year
March 1994 capability. June 1998 2000.
• Plug-in probe.
Serial Suffix ‘R’ Sample well changed.
Serial Suffix ‘D’ 512K EPROM. June 1998
May 1994
Serial Suffix ‘S’ New IEEE display and new
Serial Suffix ‘E’ Software upgrade to add adjust- October 1998 cable.
April 1995 able buzz point.

Serial Suffix ‘F’ • Added CE/ETL marks.

December 1995 • Added surge arrester to power
entry.

Serial Suffix ‘G’ Cooling assembly change.

March 1996

Serial Suffix ‘H’ Software change to correct

August 1996 silicon ID acquisition.

Serial Suffix ‘I’ ---

omitted

Serial Suffix ‘J’ Painted underside of deck.

December 1996

Serial Suffix ‘K’ Paint changed to off-white.

May 1997

Serial Suffix ‘L’ • Power supply change.

June 1997 • Discontinued CSA.

Serial Suffix ‘M’ Reduce unit reset problem.

November 1977

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 Model 4D3 Model 4D3
Cryoscope Description Cryoscope Description

No suffix Original model released. Serial Suffix ‘N’ Power supply changed.
August 1991 June 1997

Serial Suffix ‘A’ • Case manufactured in one Serial Suffix ‘O’ ---
August 1992 piece, instead of two. omitted
• Front panel (keypad) mounted
to the base assembly. Serial Suffix ‘P’ Reduction of reset problem.
• Keypad pulls down. November 1997
• Printer power output added to
the rear panel. Serial Suffix ‘Q’ Correct reboot problem.
• Head position sensors mounted January 1998
to a printed circuit board.
• Heat tansfer fluid door pulls Serial Suffix ‘R’ 4-digit year display, for year
down. June 1998 2000.

Serial Suffix ‘B’ One-way valves redesigned. Serial Suffix ‘S’ Sample well changed.
July 1993 June 1998

Serial Suffix ‘C’ • Supervisor/Operator keyswitch Serial Suffix ‘T’ New IEEE display and new
August 1993 added. October 1998 cable.
• Software change.
Serial Suffix ‘U’ • Intel Processor board set,
Serial Suffix ‘D’ • Transistors moved to the back December 2001 4D3605.
October 1993 wall. • Added Barcode port.
• New power entry module. • New power entry.
• FLASH memory upgrade.
Serial Suffix ‘E’ • Sample probe designed to plug
March 1994 into head unit. Serial Suffix ‘V’ • Added ISO 5764 seeking
• Software incorporated several April 2002 method.
languages. • New Delrin probe.

Serial Suffix ‘F’ 512K EPROM.

July 1994

Serial Suffix ‘G’ Software upgrade enabling multi-

November 1994 language translations.

Serial Suffix ‘H’ EC and ETL marks added.

October 1995

Serial Suffix ‘I’ ---

omitted

Serial Suffix ‘J’ Added surge arrester to power

December 1995 entry.

Serial Suffix ‘K’ Cooling assembly change.

February 1996

Serial Suffix ‘L’ Painted underside of deck.

December 1996

Serial Suffix ‘M’ Paint changed to off-white.

May 1997

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 Replacement Parts
3D3 4D3
Block Probe . . . . . . . . . . . . . . . . . . . .4D3340 Block Probe . . . . . . . . . . . . . . . . . . . .4D3340
Clapper . . . . . . . . . . . . . . . . . . . . . .3C2241R Clapper . . . . . . . . . . . . . . . . . . . . . .3C2241R
Control Board Set . . . . . . . . . . . . . .3D3605R Control Board Set . . . . . . . . . . . . . .4D3605R
Application Board (only) . . . . . . . .PCB605R Application Board (only) . . . . . . . .PCB605R
Processor Board (only) . . . . . . . . . .3D3620R Processor Board (only) . . . . . . . . . .4D3620R
Cooling Assembly . . . . . . . . . . . . . . .3D3300 Cooling Assembly . . . . . . . . . . . . . . .4D3300
Cooling Fan . . . . . . . . . . . . . . . . . . . .4D3360 Cooling Fan . . . . . . . . . . . . . . . . . . . .4D3360
Display Board and Harness . . . . . . . .4D3851 Display Board and Harness . . . . . . . .4D3851
EPROM suffixes “D” and later . . . .716825R EPROM suffixes “A - E” . . . . . . . . .716811R
Fluid Filter . . . . . . . . . . . . . . . . . . . . .4D3710 EPROM suffixes “F - T” . . . . . . . .716824R
Fuses Firmware “FLASH” Update
2.0 Amp . . . . . . . . . . . . . . . . . . . . . .70022 suffixes “U” and later . . . . . . . .4D3990R
1.0 Amp . . . . . . . . . . . . . . . . . . . . . .70011 Fluid Filter . . . . . . . . . . . . . . . . . . . . .4D3710
Head Up/Head down Relay . . . . . . . .3D3109 Fuses
Heat Transfer Fluid Pump Assembly .4D3690 2.0 Amp . . . . . . . . . . . . . . . . . . . . . .70022
Keypad . . . . . . . . . . . . . . . . . . . . . . . .3D3511 1.0 Amp . . . . . . . . . . . . . . . . . . . . . .70011
Load Resistor . . . . . . . . . . . . . . . . . .425150R Head Up/Head Down Relay . . . . . . .3D3109
Mandrel . . . . . . . . . . . . . . . . . . . . . . .3LH500 Heat Transfer Fluid Pump Assembly .4D3690
Motor Assembly . . . . . . . . . . . . . . . . .4D3350 Keypad . . . . . . . . . . . . . . . . . . . . . . . .4D3511
NVRAM Battery . . . . . . . . . . . . . . . .71027R Load Resistor . . . . . . . . . . . . . . . . . .425150R
One-way Check Valve . . . . . . . . . . . .4D3705 Mandrel . . . . . . . . . . . . . . . . . . . . . . .3LH500
One-Way Check Valve . . . . . . . . . . . .99133R Motor Assembly . . . . . . . . . . . . . . . . .4D3350
Power Supply Assembly . . . . . . . . . .4D3950 NVRAM Battery . . . . . . . . . . . . . . . .71027R
Probe Alignment Tool Kit . . . . . . . . .3LA700 One-Way Check Valve . . . . . . . . . . . .4D3705
Sample Probe suffixes before "C" . . .4D3700 One-Way Check Valve . . . . . . . . . . . .99133R
Sample Probe suffix "C" . . . . . . . . . .3D3700 Power Supply Assembly . . . . . . . . . .4D3950
Stir/Freeze Coil . . . . . . . . . . . . . . . . .3D2404 Probe Alignment Tool Kit . . . . . . . . .3LA700
Stir/Freeze Wire . . . . . . . . . . . . . . . . .3LH243 Sample Probe suffixes before "E" . . .4D3700
Transistor . . . . . . . . . . . . . . . . . . . .PCB520R Sample Probe suffixes "E - U" . . . . .3D3700
Transistor . . . . . . . . . . . . . . . . . . . .PCB522R Sample Probe suffix after “U” . . . . . .4D3102
Tube Ejector Spring . . . . . . . . . . . . . .3D3312 Stir/Freeze Coil . . . . . . . . . . . . . . . . .3D2404
Yoke . . . . . . . . . . . . . . . . . . . . . . . . . .3LH230 Stir/Freeze Wire . . . . . . . . . . . . . . . . .4LH243
Transistor . . . . . . . . . . . . . . . . . . . .PCB520R
Transistor . . . . . . . . . . . . . . . . . . . .PCB522R
Tube Ejector Spring . . . . . . . . . . . . . .4D3312
Yoke . . . . . . . . . . . . . . . . . . . . . . . . . .3LH230

Supplies & Accessories

3D3 4D3
100 mOsm/kg Calibration Standard -422 m°H Calibration Standard
(10 5-mL ampules) . . . . . . . . . . . .3LA011 (-408 m°C) . . . . . . . . . . . . . . . . . .3LA023
500 mOsm/kg Calibration Standard -621 m°H Calibration Standard
(10 5-mL ampules) . . . . . . . . . . . .3LA051 (-600 m°C) . . . . . . . . . . . . . . . . . .3LA033
900 mOsm/kg Calibration Standard -530 m°H Lactrol® 530 Reference
(10 5-mL ampules) . . . . . . . . . . . .3LA091 Solution (-512 m°C) . . . . . . . . . .3LA030

The Advanced® 3D3/4D3 Service Manual 32

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 3D3 4D3
1500 mOsm/kg Calibration Standard Barcode Scanner . . . . . . . . . . . . . . . . .330016
(10 5-mL ampules) . . . . . . . . . . . .3LA151 Disposable Air Filters . . . . . . . . . . . .3D2340
2000 mOsm/kg Calibration Standard Thermal Printer with Interface Cable,
(10 5-mL ampules) . . . . . . . . . . . .3LA201 Operation Manual, Thermal Paper
3000 mOsm/kg Calibration Standard Roll, and Printer Power Supply
(10 5-mL ampules) . . . . . . . . . . . .3LA301 100-120 VAC . . . . . . . . .3D3555_NA
100 mOsm/kg Calibration Standard 230 VAC . . . . . . . . . . . . .3D3555_EU
(110-mL bottle) . . . . . . . . . . . . . .3LA010 For current Thermal Printer 3D3555:
500 mOsm/kg Calibration Standard Printer Paper (5 rolls) . . . . . . . . . .3D3835
(110-mL bottle) . . . . . . . . . . . . . .3LA050 For Dot Matrix Printer 4D3550:
900 mOsm/kg Calibration Standard Paper Roll Holder . . . . . . . . . . . . .FL0408
(110-mL bottle) . . . . . . . . . . . . . .3LA090 Printer Cable . . . . . . . . . . . . . . . .3M2543
1500 mOsm/kg Calibration Standard Printer Paper, pkg rolls . . . . . . . . .330830
(110-mL bottle) . . . . . . . . . . . . . .3LA150 Printer Ribbon Cartridge, pkg . . . .330541
Clinitrol™ 290 Reference Solution Heat Transfer Fluid . . . . . . . . . . . . . .3DA811
(10 5-mL ampules) . . . . . . . . . . . .3LA029 Operator/Supervisor Keys . . . . . . . . .3D3185
Osmolality Linearity Set (5 levels) . .3LA028 Power Cord (specify voltage and
Protinol® Near Serum Reference country) . . . . . . . . . . . . . . . . . . . .----------
(3 levels) . . . . . . . . . . . . . . . . . . .3MA028 Sample Tube Rack . . . . . . . . . . . . . . .3LA846
Renol™ Urine Osmolality Controls Sample Tubes marked at 2-mL
(2 levels) . . . . . . . . . . . . . . . . . . . .3LA085 and 2.5-mL . . . . . . . . . . . . . . . . . .3LA823
Air Filters, disposable . . . . . . . . . . . .3D2340 Serial Port Computer Cable with DB9S:
Heat Transfer Fluid . . . . . . . . . . . . . .3DA811 3-Meter Shielded RS-232 . . . . . . .330053
Power Cord (specify voltage and 6-Meter Shielded RS-232 . . . . . . .330056
country) . . . . . . . . . . . . . . . . . . . .---------- 9-Meter Shielded RS-232 . . . . . . .330059
Thermal Printer with Interface Cable, Service Manual, 3D3/4D3 . . . . . . . .4D35SM
Operation Manual, Thermal Paper Supervisor Keys . . . . . . . . . . . . . . . . .3D3185
Roll, and Printer Power Supply User’s Guide . . . . . . . . . . . . . . . . . . . . .4D35
100-120 VAC . . . . . . . . .3D3555_NA
230 VAC . . . . . . . . . . . . .3D3555_EU
For current Thermal Printer 3D3555:
Printer Paper (5 rolls) . . . . . . . . . .3D3835
For Dot Matrix Printer 4D3550:
Paper Roll Holder . . . . . . . . . . . . .FL0408
Printer Cable . . . . . . . . . . . . . . . .3M2543
Printer Paper, pkg rolls . . . . . . . . .330830
Printer Ribbon Cartridge, pkg . . . .330541
Sample Tubes How to order:
(0.2 mL, disposable) . . . . . . . . . .3LA825
Sample Tube Rack . . . . . . . . . . . . . . .3LA846 To order parts, supplies and accessories, con-
Serial Port Computer Cable with DB9S: tact the Advanced Instruments Customer
3-Meter Shielded RS-232 . . . . . . .330053 Service.
6-Meter Shielded RS-232 . . . . . . .330056
9-Meter Shielded RS-232 . . . . . . .330059 • 800-225-4034 (toll-free within the USA
Supervisor Keys . . . . . . . . . . . . . . . . .3D3185 and Canada)
Service Manual, 3D3/4D3 . . . . . . . .4D35SM • +US 781-320-9000 (elsewhere)
User’s Guide . . . . . . . . . . . . . . . . . . . . .3D35 • +US 781-320-3669 (fax)

33 The Advanced® 3D3/4D3 Service Manual

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 Notes:

The Advanced® 3D3/4D3 Service Manual 34

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 3. Maintenance

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 Cleaning and Maintenance

The 3D3/4D3 cooling assembly should be 7. Insert an empty sample tube into the
cleaned once every two months to insure opti- freezing chamber and press START.
mal performance.
8. Run this test for 10-15 minutes and then
To flush the system: press STOP.

1. Open the door and remove the heat trans- 9. Remove the bottle with the diluted bleach
fer fluid bottle. and install a new bottle of heat transfer
fluid. Insert only the tube with the filter
2. Empty the contents and fill the bottle (A).
halfway with a solution of 20% bleach
and 1:5 distilled water. 10. Press START twice. Run this test until
the colored fluid begins draining out of
3. Replace the bottle and insert only the tube the return tube, and then press STOP.
with the filter (A). Completely immerse
the filter in the fluid. 11. Remove the return tube from the contain-
er and insert it into the heat transfer fluid
4. Insert the return tube (B) into another bottle. Do not immerse the return tube in
container. the heat transfer fluid (B).

5. If there is a Supervisor/Operator 12 Press STOP to exit the menu.

keyswitch, turn it to the Supervisor posi-
tion.
To drain the system:
6. Press TEST to enter the test menu. Press
1. Turn off the power and unplug the instru-
< or > to select "Head Up Down Test"
ment. Remove the cover.
and press START.
2. Remove the heat transfer fluid bottle and
empty the contents.

3. Replace the empty bottle and insert the

tubes.

4. Press gently on the pump at the bottom of

the head. Slide until all liquid has been
expelled (the unit may need to be tipped
B on its side to remove the liquid).

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 Notes:

The Advanced® 3D3/4D3 Service Manual 38

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 Osmometer/Cyryoscope Well Cleaning

Use this procedure to clean the well and tub- C B

ing of any foreign matter (e.g., broken glass)
that may have fallen into the well.

Tools needed: Flat-bladed screwdriver;

Phillips screwdriver; Tru-arc pliers; thin, stiff
wire.

Osmometer Well Cleaning

1. Turn off the power and unplug the instru-

ment.

2. Remove the two screws from the head

9. Inspect the bottom of the well and remove
cover and remove the head cover.
any visible debris.
3. Remove the screws from the side and rear
10. Using a thin stiff wire, insert it into the
panels of the instrument cover, and
tubing and the pump disconnect point in
remove the cover.
step 6.
4. Remove the four screws from the deck
Note: The wire diameter MUST be small
(A) and remove the deck.
enough to pass through the well.

A 11. Push the wire through the tubing and into

the well. Repeat this process several
times, sliding the wire back and forth.
(This should loosen any deposit trapped
in the tube and the well.)

12. Reassemble the pump to the well.

5. Drain the cooling system (see Cleaning
and Maintenance).
13 Using a solution of 20% bleach and 1:5
distilled water, place the filter and well
6. Disconnect the pump from the cooling
return line into this solution. Manually
assembly.
press the pump up and down until the
solution has circulated through the pump
7. Using a pair of Tru-arc pliers, remove the
and well, and into the bottle.
Tru-arc retainer ring (B) from inside the
well. (Save the retainer ring for replace-
14. When satisfied that the solution is free-
ment when cleaning is complete.)
flowing, stop and drain the cooling sys-
tem.
8. Remove the white Delrin elevator and
spring (C). (Save for replacement when
15. Reassemble the instrument by reversing
cleaning is complete.)
steps 1-8.

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 16. Add a new bottle of heat transfer fluid
and prime the pump. The instrument is
ready for testing.

Cryoscope Well Cleaning

1. Turn off the power and unplug the instru-

ment. Remove the heat transfer fluid from
the front cover.

2. Follow steps 2-6 from the Osmometer

Well Cleaning instruction (previous
page).

3. Remove the spring from the well and set

aside, to be replaced after cleaning.

Note the orientation of the spring as you

remove it from the well, wide-end down.

4. Follow steps 9-14 from the Osmometer

Well Cleaning instruction.

5. Replace the spring (wide-end down) into

the well and reassemble the instrument by
reversing steps 2-6 from the Osmometer
Well Cleaning instruction.

6. Add a new bottle of heat transfer fluid

and prime the pump. The instrument is
ready for testing.

The Advanced® 3D3/4D3 Service Manual 40

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 4. Troubleshooting

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 Introduction

Problems with the performance of the Before you repair or replace any
3D3/4D3 are usually identified by the soft- parts, turn the instrument off and
ware and displayed as error messages. The then back on after a short delay.
troubleshooting table on the following pages This allows the software to reinitial-
lists all error messages, indicates why the ize, and may correct what appears to
software generated the message, and lists be a serious hardware problem.
what may be causing the problem.

Error messages may be caused by mistakes CAUTION: Some troubleshooting

during testing, worn or defective parts, or requires the instrument to be turned
parts set to the wrong specifications. This on while the cover is removed. Use
section includes directions for: extreme caution when operating the
instrument without the cover.
• checking and resetting the stir & freeze
Hazardous voltages are present at
amplitude settings and the probe bin set-
the AC input, transformer, motor
tings;
capacitor, relays, and power supply.
• aligning the probe and stir-freeze wire, and
adjusting the head sensors;
• using the A/D test to check the block
probe, sample probe, and cooling assem-
bly.

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 Freeze Amplitude

To check and reset the freeze amplitude set- 5. Check the position of the yoke (B) and
ting: clapper. The yoke must be 90º and flush
against the back wall of the head. Bend
1. Turn on the instrument. If there is a the yoke if the clapper appears too close
Supervisor/Operator keyswitch, turn it to or too far away from the coil frame. The
the Supervisor position (if required). clapper does not have to be directly paral-
lel to the back wall of the unit.
2. Press TEST. Press < or > to select “Stir/
Freeze Test”, and then press START.

3. At “[stir] [freeze]”, watch the tip of the

stir/freeze wire and press >. The freeze
vibration should buzz loudly, and the
vibration blur at the tip of the wire will
last one second. The amplitude should
measure about 13mm (1/2”).

If the freeze amplitude is not within the

correct range, follow steps 4-5 below.

4. Remove the head cover and clear away

any metal chips between the coil frame
and the clapper (A).

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 Stir Driver Noise

Excessive noise in the stir amplitude and fre-

quency can affect the quality of the stir signal
and introduce excessive pre-freeze errors or
cause poor freeze pulse repeatability. The
presence of such noise can best be determined
with the use of an Oscilloscope.

1. Connect the Oscilloscope between J9-1

and ground.
2. Turn on the instrument. If there is a
Supervisor/Operator keyswitch, turn it to
the Supervisor position (if required).
Figure 2. Can be cleaned up with ferrite bead.
3. Press TEST. Press < or > to select “Stir/ Acceptable if stir wire not affected.
Freeze Test”, and then press START.
4. At “[stir] [freeze]”, press <.

The stir wire driver signal should be a clean,

square wave at approximately 72 Hertz and
4Vdc (see figure 1).

A ferrite bead placed in line with the

Darlington emitter (Q2B) does clean up the
square wave for problems, as shown in fig-
ures 2, 3 and 4.

The most serviceable place to install the fer-

rite bead is inside the head cover on the white
wire exiting the head shaft and attaching to
Figure 3. Not acceptable. Replace stir wire driver
TR1 on the head transition board. Order or use ferrite bead.
Advanced Instruments part #91035 to get the
appropriate ferrite material.

Figure 4. Not acceptable. Replace stir wire driver

Figure 1. Good signal. or use ferrite bead.

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 Head Sensor Adjustment

1. Turn off the power and unplug the instru-

ment. Remove the cover and the head
cover.

2. Manually push the head all the way down

until the head stop screw (A) meets the
base of the deck (B).

D F G

Note: If your instrument is equipped with an

Intel control board set, a series of
diagnostic LEDs have been provided to
allow visual identification of drive sig-
nals and sensor activation. The yellow
LEDs marked “Drive Up” and “Drive
Dn” indicate that the control electron-
3. The head position sensors are located on ics have issued a command to the head
the head sensor board.* To adjust the motor relays. The red LEDs marked
head sensor position, use an Allen wrench “Head Up” and “Head Dn” indicate
and loosen the two bracket screws (C), that the corresponding optical sensor
and move the board (D) and bracket (E) has been tripped.
either up or down until the head sensor
flag (F) is in the middle of the head down
sensor (G).

*If you have a 4D3 cryoscope without a

suffix, the head sensors are mounted
directly to the bracket, and not to the head
sensor board.

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 Head Up/Down Test

1. Turn on the instrument. If there is a • The sample well fluid passage (D) is not
Supervisor/Operator keyswitch, turn it to obstructed by debris, and the pump is mov-
the Supervisor position (if required). ing the heat transfer fluid into the freezing
chamber.
2. Press TEST. Press < or > to select
“Head Up/ Down Test”, and then press Note: If the sample well fluid passage is
START. obstructed, disassemble and clean the
sample well following the instructions
3. Place an empty sample tube into the found earlier in this section. Also,
freezing chamber. examine the one-way check valves (E)
to make sure they are not stuck open or
4. At “[START] Test [STOP]”, press closed.
START.
Note: If your instrument is equipped with an
The head should move continually up and Intel control board set, a series of
down. diagnostic LEDs have been provided to
allow visual identification of drive sig-
Use this test to ensure the following compo- nals and sensor activation. The yellow
nents are working properly: LEDs marked “Drive Up” and “Drive
Dn” indicate that the control electron-
• The motor (A) is running.
ics have issued a command to the head
• The gears (B) are turning properly. motor relays. The red LEDs marked
“Head Up” and “Head Dn” indicate
• The sensors (C) are detecting the head-up that the corresponding optical sensor
and head-down conditions. has been tripped.

B C D

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 Power On Diagnostics

Turn on the instrument At “Press START to 6. Press > sequentially to read:

Continue”, press START. The instrument
Block probe resistance in ohms,
performs a check of the cooling assembly and
Sample probe resistance in ohms,
the probes. If a Block Probe Failure, Sample
Block probe in degrees Celsius,
Probe Failure or Cooling System Error mes-
Sample probe in degrees Celsius, or
sage displays, use the A/D tests to perform
Cooling block duty cycle (a % value).
further testing.
Block probe resistance on instruments with
A/D Tests the Hitachi processor should be 4,990 ohms
when the target temperature is off and the
Note: You must set the correct sample and probe is warm. On instruments with the Intel
block bin numbers before performing processor, the resistance will indicate current
A/D tests. temperature. The probe begins to cool after
the target temperature is set, and the resist-
1. Turn on the instrument. If there is a ance increases until it reaches the target tem-
Supervisor/Operator keyswitch, turn it to perature.
the Supervisor position (if required).
Sample probe resistance on instruments with
2. Press TEST. the Hitachi processor should be 4,990 ohms
when the target temperature is off and the
3. Press < or > to select “A/D Tests”. Place probe is warm. On instruments with the Intel
a sample tube containing either isopropyl processor, the resistance will indicate current
alcohol or 2000 mOsm calibration stan- temperature. The probe begins to cool after
dard for a 3D3/4D3 into the freezing the target temperature is set, and the resist-
chamber, and press START. ance increases until it reaches the target tem-
perature. Due to thermal mass considerations
4. The display will change to: between the positions of the block and sample
probes, the sample probe may be ± 0.5ºC of
off Blk NNNN.NN ohm the block probe reading.

readout Duty cycle displays the percentage of time

(resistance or temperature) that the thermoelectrics power is on. When
the thermoelectrics begin to cool, the duty
channel setting cycle is usually at 100%. As the instrument
(sample or block) stabilizes, the duty cycle decreases to around
60%.
target temperature
(off, +1 or -8) Increased ambient operating temperatures do
not affect the sample probe reading, but will
increase the observed duty cycle, up to 85%
5. Set the target temperature from +1°C to at the maximum temperature of 35ºC.
-8°C by pressing < sequentially.
Variations in ambient non-condensing relative
Note: For Intel processors, the head will not humidity have no effect on the sample probe
lower until a target temperature is set. reading or duty cycle.
The head will rise when the target tem-
perature is set to off.

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 The following situations indicate a problem To determine which component is faulty:
with either the probes or the cooling assem-
bly: 1. Remove the empty tube from the freezing
chamber.
Hitachi Processor
The resistance reading is >10,000 ohms 2. Set the target temperature to -8.
on either channel without a target temper-
ature [>5,200 on 4D3 sample channel]. 3. Raise the head manually and place a ther-
This reading indicates an open circuit. mometer inside of the freezing chamber.
The resistance on either channel does not
4. If the temperature inside the freezing
increase from 4,990 ohms after the target
chamber drops to approximately -8ºC, the
temperature has been set.
cooling assembly is working, and the
main control board/application board
Intel Processor
needs to be replaced.
The resistance reading is replaced by the
number 0. Scrolling from right to left If the temperature remains at room tem-
indicates an open circuit. perature, replace the cooling assembly.
The target temperature cannot be reached,
Note: When the A/D channel is open, there is
but probe readings that vary around ambi-
a thirty-second delay in response to
ent indicate a cooling system problem.
any resistance change.
To determine if a faulty probe is causing the
CAUTION: If the A/D test sample freezes,
problem:
allow it to thaw before attempt-
ing to remove the sample probe.
1. Disconnect the probe from the main
board.

2. Place a 6 Kohm fixed resistor across pins Sample Bin Setting

1 and 2.
If you do not know the sample bin number, or
3. If the resistance display changes to if you want to verify the sample bin number:
approximately 6,000 ohms, replace the
probe. 1. Turn on the instrument. If there is a
Supervisor/Operator keyswitch, turn it to
If the resistance does not change, replace
the Supervisor position (if required).
the main control board or application
board.
2. Press TEST. Press < or > to select
“Probe Bin Test”, and then press
If the resistance on both channels does not
START.
increase from 4,990 ohms on Hitachi proces-
sor systems, or if the temperature reads
3. Place a sample of Probe Bin-Setting Fluid
approximately ambient on Intel processor sys-
into the freezing chamber.
tems while a target temperature is set, the sit-
uation indicates a problem with either the
4. At “[START] Ready?”, press START.
cooling assembly, main control board, or
application board.

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 5. At the end of the test, record the sample Note: If you have a 4D3 cryoscope with a
probe resistance and the bin number, and suffix before “C”, the menu item “Set
then press STOP. The display will Block Bin #” is within the TEST menu,
change to “Probe Bin Test”. not the SETUP menu.

To set the correct sample bin number, fol- 4. At “Select Setup Item”, press < or > to
low steps 6-8. select “Select Block Bin #”, and then
press START.
If the current sample bin number matches
the number just recorded, press STOP 5. Enter the correct number and then press
twice to exit the menu. ENTER.

6. Press SETUP. The Supervisor/Operator 6. Press STOP to exit the menu.

keyswitch must be in the Supervisor posi-
tion.
Stir Amplitude
Note: If you have a 4D3 cryoscope with a
suffix before “C”, the menu item “Set
1. Turn on the instrument. If there is a
Sample Bin #” is within the TEST
Supervisor/Operator keyswitch, turn it to
menu, not the SETUP menu.
the Supervisor position (if required).
7. At “Select Setup Item”, press < or > to
2. Press SETUP. Press < or > to select “Set
select “Select Sample Bin #”, and then
Stir Amplitude”, and then press START.
press START.
3. Watch the vibration blur at the tip of the
8. If the current sample bin number is not
stir/freeze wire. If the amplitude is not
the same as the one recorded in step 5,
within the range specified below, press >
enter the correct number, press ENTER,
to increase the amplitude, or press < to
and then press STOP twice to exit the
decrease the amplitude. (The default is
menu.
98, but your value may differ.)

3D3 The stir wire movement should be

Block Bin Setting
no wider than the width of the sam-
ple probe.
1. Turn off the power and unplug the instru-
ment. Remove the cover and record the 4D3 The stir wire movement should be
block probe bin number located on the twice the width of the sample
block probe wire. probe.

2. Replace the cover. Plug in the instrument 4. Check the setting by placing a sample
and turn it on. If there is a Supervisor/ (water or standard solution) in position on
Operator keyswitch, turn it to the the probe. If bubbles form in the sample,
Supervisor position (if required). or if the stir wire hits the tube, the current
setting is too high and must be adjusted
3. At “Press START to Continue”, press downward.
SETUP.
5. When the setting is correct, record the
new stir amplutide number for your
records, and press ENTER.

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 6. At “Set Stir Amplitude”, press STOP to If you do not hear the beeper and the
return to “Osmometer (or Cryoscope) instrument is working otherwise, the
Ready”. application board may need to be replaced
(see Replacement Instructions for addi-
Note: Stir wire movement should be stable. tional information).
If movement has erratic amplitude,
driver transistor Q2B may need 3. Press STOP twice to end the test and exit
replacement. Use an oscilloscope at the Test menu.
J9-1 to verify the presence of a clean,
square wave (see Stir Driver Noise
procedure).
Keypad Test

1. Turn on the instrument. If there is a

Display/Printer Test Supervisor/Operator keyswitch, turn it to
the Supervisor position (if required).
1. Turn on the instrument. If there is a
Supervisor/Operator keyswitch, turn it to 2. Press TEST. Press < or > to select
the Supervisor position (if required). “Keypad Test”, and then press START to
activate the keypad test. Each key
2. Press TEST. Press < or > to select pressed should respond on the display
“Display/Printer Test”, and then press with the key name, and activate the corre-
START twice. sponding LED, if present.
A continuous string of characters and If any keys or LEDs do not respond,
numbers should scroll across the display, check the narness connections and repeat
and a single string of the same characters the test. Otherwise, the keypad or appli-
and numbers will be sent to the printer. cation board may need to be replaced (see
Replacement Instructions for additional
If not, check your connections and try
information).
again. If you continue to have a problem
and the instrument is working otherwise,
3. Press STOP twice to end the test, and
the printer or display may need to be
STOP again to exit the Test menu.
replaced (see Replacement Instructions
for additional information).

3. Press STOP twice to end the test and exit Barcode Test
the Test menu. (Only Intel instruments with a barcode con-
nector.)

1. Turn on the instrument.

Beeper Test
2. Press TEST. Press < or > to select
1. Turn on the instrument. If there is a
“Barcode Test”, and then press START
Supervisor/Operator keyswitch, turn it to
to activate the barcode test. Using the
the Supervisor position (if required).
attached scanner, begin scanning bar-
codes. Each scan should display on the
2. Press TEST. Press < or > to select
instrument.
“Beeper Test”, and then press START
and < to activate the continuous internal If the barcode does not display, check the
beeper. connections. Try resetting the scanner to

51 The Advanced® 3D3/4D3 Service Manual

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 the factory defaults using the scanner
manual or included Advanced Instruments
documentation. Verify with the scanner
manual that the type of barcode you are
using is activated.

If you are still having problems, contact

Advanced Instruments for service.

3. Press STOP twice to end the test and exit

the Test menu.

Reset Serial Number

The instrument serial number appears when

you power up the instrument. If you need to
reset the serial number:

1. If there is a Supervisor/Operator
keyswitch, turn it to the Supervisor posi-
tion (if required).

2. At “Press START to Continue”, press

SETUP.

3. Cycle through the menu options by press-

ing < or > until you reach “Serial
Number”, and then press START. The
display will read “Serial#:********”.

4. Press the following keys consecutively:

2, 4, CLEAR. The displayed serial num-
ber will disappear.

5. Enter each digit of the serial number as it

appears on the label on the back of the
instrument (Note: the letter suffix at the
end of the serial number will not be
entered). When the number is correct,
press ENTER.

The serial number will be recorded in

memory and should appear the next time
you power up your instrument.

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 Instrument Software Updates

Software for Advanced Instruments model Performing FLASH firmware update

3D3/4D3 instruments is contained in factory-
installed integrated circuits called PROMs, Performing this update should not affect any
EPROMs, or Flash EPROMs, and is some- of your calibrations or system settings unless
times referred to as “Firmware”. the update has made changes to this area of
memory. For specific information, consult
PROM stands for “Programmable Read-Only the documentation included with the update.
Memory”, and is a one-time use device.
EPROMs are “Erasable Programmable Read- For Intel-based instruments
Only Memory”, and can be reused by expos-
If your instrument has a three-position (metal
ing the device to ultraviolet light and an
housing) Operator/Supervisor keyswitch, then
external reprogramming sequence. Both of
it was purchased with the Intel processor con-
these devices have been used on Advanced
trol board set 3D3605/4D3605.
Instruments model 3D3/4D3 instruments with
the Hitachi processor in DIP (Dual In-Line
1. Turn off the instrument. Select the 45º
Package) byte densities of 256K and 512K
position between Operator and
(see section 2, System control electronics to
Supervisor.
learn how to identify your memory type).
2. Attach the Advanced Instruments serial
The 256K Byte Hitachi PROM/EPROM
cable between your PC and the instru-
structure is no longer supported and cannot be
ment.
updated. The 512K Byte PROM/EPROM-
based system can be updated on a limited
3. Turn on the instrument. The display
basis. Complete updates, allowing for contin-
should read “Flash BOOT Program”.
ued support, require replacing the control
electronics with the new Intel control board
4. Perform the download, using the instruc-
set.
tions provided with the update software.
The new Intel-based instruments use Flash After download is complete, the instru-
EPROMs, “Electrically Erasable Program- ment will reboot and report the new soft-
mable Read-Only Memory”. This type of ware version.
memory is furnished in two PLCC (Plastic
Lead Chip Carrier) devices on the 3D3620/ 5. Turn off the instrument and return the
4D3620 processor board at locations U3 and Operator/Supervisor keyswitch to the
U4. U3 is the lower 8 bits of the 16-bit desired position.
processor address bus, and U4 is the upper 8
bits.
For Hitachi systems with Intel upgrade
Advanced Instruments products using this
“FLASH” technology can be field-updated If your instrument has a two-position (black
using a Windows PC and Advanced plastic) Operator/Supervisor keyswitch, then
Instruments serial cable. For the latest it was purchased with the Hitachi processor
instructions on performing the RS-232 port control board 3D3600/4D3600. However, if
firmware upgrade, consult the documentation you have replaced that board with the Intel
supplied with the upgrade package. For infor- processor control board set 3D3605/4D3605,
mation on available updates, consult you can still perform the field firmware
Advanced Instruments or your dealer. update using the following procedure.

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 Note: Since this procedure requires opening download again. If after download the instru-
the case, this update should be per- ment does not function correctly, consult the
formed only by an authorized techni- rest of this service manual before contacting
cian. Advanced Instruments for service.

1. Turn off the power and unplug the instru- Option Switch Settings:
ment. Processor board 3D3620/4D3620 is equipped
with a three-position dip switch. Each switch
2. Remove the instrument cover (see section position and combination of positions can be
2 for instructions on removing the instru- used to configure different programming
ment cover). options, such as the programing option listed
above for instruments without the three-posi-
3. Locate the 3D3620/4D3620 processor tion external keyswitch. If your settings
board (smaller of two). should accidentally get changed, or your
replacement processor board is shipped from
4. Locate SW1 dip switch on the processor the factory with the incorrect settings for your
board. Locate switch position 3 (lower product, the following table details the appro-
switch) and select the CLOSED, ON, or 1 priate switch positions for restoring your
position. Do not change any other switch instrument to proper operation.
setting.
Option Switch Setup Table
5. Attach the Advanced Instruments serial
cable between your PC and the instru-
ment. SW1
1 2 3
6. Plug in and turn on the instrument. The 3D3 ON OFF OFF
display should read “Flash BOOT MK3 ON ON OFF
Program”. 4D3 OFF OFF OFF
MK2 OFF ON OFF
7. Perform the download, using the instruc- Program N/A N/A ON
tions provided with the update software. NOTE:
ON = 1 = CLOSED
OFF = 0 = OPEN
After download is complete, the instru-
ment will reboot and report the new soft-
ware version.

8. Turn off the power and unplug the instru-

ment.

9. Restore SW1 position 3 to the OPEN,

OFF, or 0 position.

10. Replace the instrument cover.

11. Reattach the power cord and return the

instrument to use.

If any problems occur, check the connections

and switch position settings, and then try the

The Advanced® 3D3/4D3 Service Manual 54

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 Troubleshooting Table
Symptom Explanation Suggested Action
1. Abrupt loss of power Interrupted power connection or loss of 1. Make sure the power cord is firmly
(no display) power. plugged into both the wall outlet and the
socket on the back of the instrument.
2. Temporarily unplug the power cord from
its wall outlet and replace it with a known
workable lamp or small appliance. Have
the power restored.
3. Measure the AC voltage supplied at the
power outlet used for this instrument.
4. Unplug the power cord from the power
cord socket. Visually check the fuses for a
blown fuse. If there is any doubt, test the
fuses with a continuity checker or ohm-
meter or simply replace them.
5. Check the values marked on the fuses.
6. Replace blown or incorrect-value fuses
with time delay (T) fuses: 2-Amp fuses
for 100-130V operation or1-Amp fuses
for 200-250V operation.
7. If a fuse was blown and a replacement
fuse blows too, contact our product serv-
ice department for assistance.

2. “A/D Init Failure” One or both A/D converters lost initial- 1. Turn power off and on.
“A/D High Filter Error” ization. 2. If problem persists, replace application
“A/D Low Filter Error” board.
“A/D Cal Mode Error”

3. “Baudrate Error” The baudrate setting is out of range. Reset baudrate in SETUP menu.

4. “Block Probe Failure” The block probe has failed (usually Check the block probe resistance (2,300
shorted). ohms ±300 at room temp.)

5. "Block Probe Open?" The block probe resistance is greater 1. Is the probe plugged in?
than A/D converter can measure. 2. Check the block probe resistance (2,300
ohms ±300 at room temp.)

6. “Calibration Cancelled” The STOP button was pressed during Calibration cancelled.
calibration.

7. “Calibration Out of The range of the samples tested did not 1. Recalibrate the instrument.
Range; Please Repeat” repeat well enough for calibration. 2. Check freeze pulse.
“Calibration Out of 3. Check sample size and quality.
Range; Repeat Calib” 4. Check probe/size alignment.
“Calibration Out of
Range; Repeat”

8. "Check Calibration" Buzz point setting has been changed. 1. Spot-check calibration.
(3D3 only) 2. Recalibrate, if needed.

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 Symptom Explanation Suggested Action

9. “Cooling System The cooling assembly may not be func- 1. Check the cooling assembly.
Error” tioning properly. 2. Make sure there is heat transfer fluid in
the cooling well.

10. “Count Error; A memory check error occurred in the 1. Turn the power off and on.
Parameter RAM” RAM. 2. If problem persists, replace processor
board.

11. "Error Reading The data received from the barcode 1. Turn the power off and on.
Barcode” reader was not formatted correctly. 2. Check barcode scanner settings.
3. If problem persists, replace application
board.

12. “Event Record Lost” The instrument has lost its record from 1. Turn the power off and on.
memory. 2. If problem persists, replace processor
board.

13. “Fan Driver Failure” The fan is not functioning properly. 1. Check fan driver/control circuit.
“Fan System Failure” 2. Replace the fan or application board.

14. “Flash Boot Program” The instrument is configured to receive 1. If intentional, perform download process
Flash EPROM updated code. as described in this manual.
2. If not intentional, turn off power and set
three-position Operator/Supervisor key-
switch to Operator or Supervisor position.
3. Check processor board option switch
SW1 position 3 and set to OPEN/OFF/0
(see Option Switch Setup Table).

15. “Head Not Up” The head up/down mechanism may not 1. Check the motor, clutch assembly and
“Head Not Down” be functioning properly. relays by running the head up/down test.
2. Check the Head Up or Head Down posi-
tion sensor.
3. Check LED indicators on application
board.

16. “New Software EPROM has been changed, or new soft- For Hitachi systems:
Version” ware has been downloaded. 1. Reset the sample and block bin numbers.
2. Reset the stir amplitude.
3. Recalibrate the instrument.
4. Reset plateau mode/range.
5. Reset instrument serial number.
6. If problem persists, replace processor
board.

17. “No Parameters in The battery-backed RAM has lost its 1. Reset sample and block probe bin num-
RAM” data or has been cleared. bers. Recalibrate the instrument.
“Parameter RAM 2. Reset the stir amplitude.
Failed” The instrument has lost the calibration, 3. Reset the buzz point (3D3 only).
data, and probe bin numbers from mem- 4. Reset plateau mode/range.
ory. 5. Reset instrument serial number.
6. If problem persists, replace processor
board.

The Advanced® 3D3/4D3 Service Manual 56

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 Symptom Explanation Suggested Action

18. “No Plateau Detected, The sample did not reach a valid 1. Make sure the sample is not too viscous.
Please Repeat” plateau. 2. Check the sample probe.
“No Plateau, Repeat 3. Make sure there is heat transfer fluid in
Test” the cooling well.

19. “Overrange, Use High The sample tested in the low range set- Retest the sample in the high range setting.
Range” ting was over the low range limit.
“Result >2000, Use
High Range”
“Result >2500, Use
High Range”
(3D3 only)

20. “RAM Failure” The main board has failed. Replace the processor board.
“EPROM Failure”

21. “Recalibration The calibration data has been lost from- 1. Recalibrate the instrument.
Needed” memory. This is caused by: 2. If error message persists after successful
a. battery back-up failure, calibration, replace the processor board.
b. installation of a new processor
board,
c. corruption of system memory, or
d. new software/firmware.

22. “Reset Probe The probe bin data has been lost from 1. Reset sample and block probe bin num-
Configuration” memory. This is caused by: bers.
a. battery back-up failure, 2. If problem persists, replace processor
b. installation of a new processor board.
board,
c. corruption of system memory, or
d. new software/firmware.

23. “Result = 0, Bad A. The sample tested was de-ionized 1. Recalibrate the instrument.
Calibration?” H2O (this is not an error). 2. Check the sample/block probe bin set-
“Result = 0, tings.
Calibration Okay?” B. The instrument was not recalibrated
(Hitachi processor only) after the sample probe was replaced.

C. The sample/block probe bin settings

are incorrect.

24. “Result >4000, Out of The sample tested was beyond the range Dilute the sample and retest.
Range” (3D3 only) of the instrument.

25. “Sample Did Not The sample did not freeze (after 1. Make sure the sample tube is not empty,
Freeze, Please Repeat” “buzzing”). and make sure the sample is not too vis-
“Sample Did Not cous.
Freeze, Repeat Test” 2. Check the freeze amplitude.
3. Make sure the instrument is in the proper
range (3D3 ony).

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 Symptom Explanation Suggested Action

26. “Sample Freeze Error, The sample did not freeze properly. 1. The sample may have been contaminated.
Repeat” Retest with a fresh sample.
“Sample Freeze Error, 2. Check the freeze amplitude.
Repeat Test” 3. Check the sample probe.

27. “Sample Pre-Freeze, The sample has frozen prematurely 1. The sample may have been contaminated.
Please Repeat” (before the “buzz”). Retest with a fresh sample.
“Sample Pre-Freeze, 2. Check sample/block probe bin settings.
Repeat Test” 3. Check the stir amplitude.
4. Check for stir driver noise.

28. “Sample Probe The sample probe has failed (usually Check the sample probe resistance (2,300
Failure” shorted). ohms ±300 at room temp).

29. “Sample Probe Open?” The sample probe resistance is greater 1. Is the probe plugged in?
than 10558 ohms. 2. Check the sample probe resistance (2,300
ohms ±300 at room temp).

30. “Serial Loop Failed” RS-232 communications test failed. 1. Was loop back connector attached?
(Hitachi processor only) 2. Try lower baud rate.
3. If problem persists, consult factory serv-
ice.

31. “Standards Reversed? The instrument was expecting “high” Recalibrate the instrument with the calibrator
Please Repeat” calibrator standard and detected a value standards in the correct order.
“Standards Reversed? below, equal or close to the “low” cali-
Repeat Calib” brator standard.

32. “Stir/Freeze Failure” The stir/freeze mechanism may not be 1. Check the stir and freeze ampligudes.
“Stir System Failure” functioning properly. 2. Check stir driver/control circuits.
3. If problem persists, replace the processor
board.

33. “Supervisor Key Operator/Supervisor keyswitch is in the 1. Press the STOP key.
Needed, Press Stop” Operator position. 2. Set the keyswitch to the Supervisor posi-
tion.

34. “System Error: Trap” The software did not execute properly. 1. Turn the power off and on.
“System Error: NMI” 2. If problem persists, replace the processor
“System Error: ID” board.
“System Error: Unknown”
“System Error: Requests”
“System Error: Primitive”
“System Error: Interrupt”
“System Error:
Communications”
“Illegal Error Code:
“Unknown Error”
“Memory Allocation
Error”
“ROM Serial Number
Error”

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 Symptom Explanation Suggested Action

35. “TE Driver Failure” The thermoelectric driver may not be 1. Use the A/D test to check the cooling
“Thermoelectrics functioning properly. assembly.
Driver Failure” 2. Check Q1B driver.
“Thermoelectrics 3. Check application board.
System Failure”

36. “Test Cancelled” The STOP button was pressed during Retest with a fresh sample.
the test.

37. “Test Time-out Error” The test has taken too long to complete. 1. Make sure the sample tube is not empty,
and make sure the sample is not too vis-
cous.
2. Make sure there is heat transfer fluid in
the cooling well.
3. Check the stir amplitude.
4. Check the sample/probe block bin setting.
5. Check the cooling assembly well.
6. Use A/D test to verify cooling system
function.

38. “Underrange, Use Low The sample tested in the high range set- Retest the sample in the low range setting.
Range” ting was under 1400 mOsm.
“Result <1400, Use
Low Range”
(3D3 only)

39. Wrong instrument Processor board 3D3620/4D3620 option Reset switch SW1 (see Option Switch Setup
model displayed switch SW1. Table).

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 Notes:

The Advanced® 3D3/4D3 Service Manual 60

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 5. Replacement
Instructions

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Downloaded from www.Manualslib.com manuals search engine
 Block Probe Replacement Kit: Models 3D3/4D3
4D3340
Reference: Use this instruction with replace- c. Remove the four screws from under-
ment part 4D3340. neath the front cover, lift the front
cover slightly up and then slide it for-
Tools Needed: Phillips screwdriver, flat- ward and off.
bladed screwdriver.
3. Remove the four screws from the deck
(A), and remove the deck.
Warning-Hazardous Voltage
Note: If you have a 4D3 cryoscope with no
Warning-Internal components may suffix, there are seven screws on the
be damaged by static electricity. deck that you must remove.

CAUTION: Improper connections A

may cause damage to the instrument.

Instruction:

1. Turn off the power and unplug the instru-

ment.

2. Remove the screws securing the instru-

ment cover, and remove the cover (see
below):
4. Disconnect the block probe from the main
The following instructions refer to 4D3
circuit board.
cryoscopes with suffix "A" or later, and
to all 3D3 osmometers:
5. Remove the cable clamp screw and the
a. Remove the two sets of screws from cable clamp that secures the block probe
the side panels and the two screws cable to the heatsink.
from the rear panel.
6. Notice the length of probe cable as you
b. Slide the instrument cover up and off. remove it from the block. Cut any tie
wraps used to create the service loop, and
The following instructions refer to 4D3 then remove the block probe cable from
cryoscopes without a suffix: the Cooling Assembly.
a. Remove the heat transfer fluid from
the front cover. 7. Insert the tip of the thermal grease appli-
cator all the way into the block probe
b. Remove the two screws from the rear hole (B). Inject grease into the hole
panel of the back cover and lift off the while slowly withdrawing the tip, so that
back cover. there are no air pockets. Fill the hole

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4SP340 Rev3
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 10. Place the cable tie (C) on the stand-off so
the tie lock faces up. Insert the washers
and the screw into the stand-off (D).
B
Tighten securely.

11. Wrap the cable tie down and around the

block probe cable and up through the tie
lock (E). Pull the cable tie to tighten.
Cut the excess cable tie above the tie
lock.

12. Make sure the block probe cable is secure

at the stand-off, and make sure it is fas-
tened in the block probe hole.

13. Reassemble by reversing steps 1-4.

14. Set the new block bin number (see the

with grease. (The thermal grease should following procedure).
surround the probe when it is inserted.)
Set Block Bin Number
8. Insert the new block probe cable into the
block probe hole until it will not go any 1. If there is a Supervisor/ Operator
further. Insert slowly so the probe is not keyswitch, turn it to the Supervisor posi-
damaged, allowing the thermal grease to tion (if required).
be displaced by the probe. Once fully
inserted, hold the probe cable in place 2. At “Press START to Continue”, press
until excess grease has stopped oozing out SETUP.
and the block probe remains in place.
Wipe away any excess grease. Note: If you have a 4D3 cryoscope with a
suffix before “C”, the menu item “Set
9. Note the block bin number located on the Block Bin #” is within the TEST menu,
block probe cable (see step 13). not the SETUP menu.

3. At “Select Setup Item”, press < or > to

select “Select Block Bin #”, and then
E press START.

D 4. Enter the correct number and then press

ENTER.

5. Press STOP to exit the menu.

6. Check calibration and recalibrate, if nec-

essary.

C
4SP340 Rev3
(4D35 Service Manual)
Page 2 of 2

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 Clapper Replacement
3C2241R

Reference: Use this instruction with replace- 6. Replace the old clapper with the new one
ment part 3C2241R. and reassemble by reversing steps 2-5.

Tools Needed: Phillips screwdriver, flat- 7. Use the probe alignment tool and instruc-
bladed screwdriver, 5/64” tions to properly position the probe and
Allen wrench, probe align- stir/freeze wire.
ment tool and instructions.

CAUTION: Improper connections may

cause damage to the instrument. B D

Instruction:

1. Turn off the power and unplug the instru-

ment.
C
2. Remove the two screws from the head
cover (A) and lift off the head cover.

A 4250 / 4D3 Cryoscope

D
B

3. Remove the two stir/freeze wire setscrews

(B) from the clapper.

4. Gently pull the stir/freeze wire (C) down C

and out of the clapper.

5. Remove the two screws (D) that attach

the clapper to the yoke. 3250 / 3D3 Osmometer

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 3C2P241R Rev5
Service Center (U.S. 1-800-225-4034, outside (4D35/3255 Service Manual)
North America +US 1-781-320-9000). Page 1 of 1

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Downloaded from www.Manualslib.com manuals search engine
 Control Board Set Replacement
3D3605R/4D3605R
Application Board (Only) Replacement
PCB605R
Processor Board (Only) Replacement
3D3620R/4D3620R

Reference: Use this instruction with replace- The following instructions have been pre-
ment part 3D3605R, 4D3605R, pared to cover several different replacement
PCB605R, 3D3620R, or scenarios. Please read carefully and select the
4D3620R. appropriate set of instructions for your situa-
tion.
Tools Needed: Phillips screwdriver, flat-
bladed screwdriver, static GENERAL INSTRUCTIONS:
grounding (earthing) wrist
strap (included). 1. Record current sample and block probe
bin numbers, sample stir amplitude, and
any other customized settings that may
Warning-Hazardous Voltage need to be reset after this procedure.

Warning-Internal components may 2. Turn off the power and unplug the instru-
be damaged by static electricity. ment.

CAUTIONS: 3. Remove the screws securing the instru-

ment cover, and remove the cover (see
• Power cord must be disconnected
below):
to prevent electric shock.
• Never unpack, touch or handle any The following instructions refer to 4D3
integrated circuit without wearing cryoscopes with suffix "A" or later, and
a grounding (earthing) strap to to all 3D3 osmometers:
minimize your static charge.
a. Remove the two sets of screws from
• Instrument calibration and all cus- the side panels and the two screws
tomized settings will be lost when from the rear panel.
the main control board, board set,
b. Slide the instrument cover up and off.
or processor board is replaced.
Therefore, make sure the current
The following instructions refer to 4D3
sample and block probe bin num-
cryoscopes without a suffix:
bers and the sample stir amplitude
are recorded in the service log so a. Remove the heat transfer fluid from
that you can reset these vital num- the front cover.
bers into the replacement board.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4D3P620 Rev3
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 4

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 b. Remove the two screws from the rear 3. Remove the replacement board set from
panel of the back cover and lift off the its anti-static bag, taking care not to dis-
back cover. turb the options/configuration switch
SW1 (A), located on the processor board
c. Remove the four screws from under-
(smaller of two).
neath the front cover, lift the front
cover slightly up and then slide it for-
4. Press the new board set onto the four
ward and off.
snap-on standoffs so that the words
“Advanced Instruments, Inc.” are located
4. Attach the static grounding strap to your
in the upper left corner.
wrist and stick the adhesive end to a suit-
able bare-metal ground, such as the rear
5. Reconnect all wiring connections and
panel.
proceed to the REASSEMBLE AND
CHECKOUT instruction, below.
INSTRUMENTS WITH A TWO-BOARD
SET:
Application Board (larger of two)
Replacement:
Board Set Replacement:
1. Remove the wiring connectors from the
1. Remove the wiring connectors from the application board (larger of two).
application board (larger of two).
2. Gently pry the board set off the four
2. Gently pry the board set off the four snap-on standoffs.
snap-on standoffs.
A

B C

Two-Board Set
4D3P620 Rev3
(4D35 Service Manual)
Page 2 of 4

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 3. Remove the processor board (smaller of INSTRUMENTS WITH A SINGLE
two) by first releasing the two plastic CONTROL BOARD:
standoffs at locations E3 and E4 (B), tak-
(Control Board Set 3D3605R/4D3605R
ing care not to disturb the
replaces Main Control Board 3D3600/
options/configuration switch SW1 (A).
4D3600.)
Next, gently pry the processor board off
its J1 and J2 connectors (C) by rocking it
1. Remove the wiring connectors from the
from top to bottom.
control board.
4. Remove the replacement application
2. Gently pry the board set off the four
board from its anti-static bag.
snap-on standoffs.
5. Install the processor board onto the new
3. Remove the replacement board set from
application board by reversing step 3,
its anti-static bag, taking care not to dis-
being careful to firmly seat the J1 and J2
turb the options/configuration switch
connections and aligning pins to sockets.
SW1 (A).
6. Press the new board set onto the four
4. Press the new board set onto the four
snap-on standoffs so that the words
snap-on standoffs so that the words
“Advanced Instruments, Inc.” are located
“Advanced Instruments, Inc.” are located
in the upper left corner.
in the upper left corner.
7. Reconnect all wiring connections and
5. Reconnect all wiring connections and
proceed to the REASSEMBLE AND
proceed to the REASSEMBLE AND
CHECKOUT instruction, below.
CHECKOUT instruction, below.

Processor Board (smaller of two)

REASSEMBLE AND CHECKOUT
Replacement:
1. Verify that all wiring connections have
1. Remove the processor board (smaller of
been restored, and that they are not offset
two) by first releasing the two plastic
on the mating board’s connector.
standoffs at locations E3 and E4 (B).
Next, gently pry the processor board off
2. Remove the static ground strap and put
its J1 and J2 connectors (C) by rocking it
aside until checkout is complete.
from top to bottom.
3. Replace the cover, taking care not to
2. Remove the replacement processor board
pinch any wiring.
from its anti-static bag, taking care not to
disturb the options/configuration switch
4, Verify that the power switch is OFF, and
SW1 (A).
then plug in the power cord.
3. Install the new processor board onto the
5. Turn on the instrument and verify that the
new application board by reversing step
instrument boots up correctly by watch-
1, being careful to firmly seat the J1 and
ing the display and/or printout.
J2 connections.
If you replaced the board set, processor
4. Proceed to the REASSEMBLE AND board, or an older single control board
CHECKOUT instruction, below.
4D3P620 Rev3
(4D35 Service Manual)
Page 3 of 4

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 configuration, you will need to enter your Reset Serial Number
configuration settings and recalibrate your
instrument (next step). 1. If there is a Supervisor/Operator
keyswitch, turn it to the Supervisor posi-
If you are using the original processor tion (if required).
board (application board replacement),
you will not need to reenter any settings 2. At “Press START to Continue”, press
and may skip to step 8. SETUP.

6. Use the SETUP menu to reset the sample 3. Cycle through the menu options by press-
and block probe bin numbers and the ing < or > until you reach “Serial
sample stir amplitude, and any other cus- Number”, and then press START. The
tomized settings. display will read “Serial#:********”.

Note: If values are unavailable, refer to the 4. Press the following keys consecutively:
User’s Guide for setup procedures. 2, 4, CLEAR. The displayed serial num-
ber will disappear.
7. Reset the serial number (see the following
procedure). 5. Enter each digit of the serial number as it
appears on the label on the back of the
8. Press STOP to exit the Setup menu. The instrument (Note: the letter suffix at the
instrument will run the self-diagnostics end of the serial number will not be
program. entered). When the number is correct,
press ENTER.
9. Recalibrate the instrument. For further
information, refer to your User’s Guide. The serial number will be recorded in
memory and should appear the next time
10. If you have upgraded from a single con- you power up your instrument.
trol board to the two-board set, see the
Supplemental Information document.

11. If you have upgraded from a single con- XXX620 Option Switch Setup
trol board to the two-board set and your
instrument has the black plastic keyswitch SW1
located on the rear of the instrument, your 1 2 3
Operator and Supervisor positions will 3D3 ON OFF OFF
have changed. Replacement labels have MK3 ON ON OFF
been provided along with this upgrade. 4D3 OFF OFF OFF
MK2 OFF ON OFF
Place the “OPERATOR” label along the Program N/A N/A ON
top of the keyswitch, and place the NOTE:
ON = 1 = CLOSED
“SUPERVISOR” label along the right OFF = 0 = OPEN
side.

4D3P620 Rev3
(4D35 Service Manual)
Page 4 of 4

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 Cooling Assembly Replacement
3D3300/4D3300

Reference: Use this instruction with replace- b. Remove the two screws from the rear
ment part 3D3300 or 4D3300. panel of the back cover and lift off the
back cover.
Tools Needed: Phillips screwdriver, flat-
c. Remove the four screws from under-
bladed screwdriver.
neath the front cover, lift the front
cover slightly up and then slide it for-
ward and off.
Warning-Hazardous Voltage
3. Drain the system:
Warning-Internal components may
be damaged by static electricity. a. Remove the heat transfer fluid bottle
and empty the contents.
CAUTION: Improper connections
may cause damage to the instrument. b. Replace the empty bottle and insert
the tubes.
c. Press gently on the pump at the bot-
Instruction: tom of the head. Slide until all liquid
has been expelled (the unit may need
1. Turn off the power and unplug the instru- to be tipped on its side to remove the
ment. liquid).

2. Remove the screws securing the instru-

ment cover, and remove the cover (see 4. Remove the deck and disconnect the
below): pump (see below):

The following instructions refer to 4D3 The following instructions refer to 4D3
cryoscopes with suffix "A" or later, and cryoscopes with suffix "A" or later, and
to all 3D3 osmometers: to all 3D3 osmometers:

a. Remove the two sets of screws from a. Remove the four screws from the
the side panels and the two screws deck (A), and remove the deck.
from the rear panel. b. Remove the two screws that secure
b. Slide the instrument cover up and off. the bottle bracket to the cooling
assembly (B).
The following instructions refer to 4D3 c. Remove the two screws that secure
cryoscopes without a suffix: the pump bracket to the cooling
a. Remove the heat transfer fluid from assembly (C).
the front cover. d. Disconnect the hose from the pump
mechanism (D).

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4D3P300 Rev1
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 A 5. Unplug the thermoelectric connector and
the block probe from the main control
board.

6. Lift out the old cooling assembly and

replace with the new one.

7. Reassemble the instrument by reversing

steps 1-5.

C B

The following instructions refer to 4D3

cryoscopes without a suffix:
a. Remove the seven screws from the
deck and remove the deck.
b. Disconnect the hose from the pump
mechanism.
c. Remove the wingnut that secures the
two brackets to the chassis, and then
remove the brackets.

4D3P300 Rev1
(4D35 Service Manual)
Page 2 of 2

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 Cooling Fan Replacement
4D3360
Reference: Use this instruction with replace- b. Remove the two screws from the rear
ment part 4D3360. panel of the back cover and lift off the
back cover.
Tools Needed: Phillips screwdriver, flat-
c. Remove the four screws from under-
bladed screwdriver.
neath the front cover, lift the front
cover slightly up and then slide it for-
ward and off.
Warning-Hazardous Voltage
3. Drain the system:
Warning-Internal components may
be damaged by static electricity. a. Remove the heat transfer fluid bottle
and empty the contents.
CAUTION: Improper connections
may cause damage to the instrument. b. Replace the empty bottle and insert
the tubes.
c. Press gently on the pump at the bot-
Instruction: tom of the head. Slide until all liquid
has been expelled (the unit may need
1. Turn off the power and unplug the instru- to be tipped on its side to remove the
ment. liquid).

2. Remove the screws securing the instru-

ment cover, and remove the cover (see 4. Remove the deck and disconnect the
below): pump (see below):

The following instructions refer to 4D3 The following instructions refer to 4D3
cryoscopes with suffix "A" or later, and cryoscopes with suffix "A" or later, and
to all 3D3 osmometers: to all 3D3 osmometers:

a. Remove the two sets of screws from a. Remove the four screws from the
the side panels and the two screws deck (A), and remove the deck.
from the rear panel. b. Remove the two screws that secure
b. Slide the instrument cover up and off. the bottle bracket to the cooling
assembly (B).
The following instructions refer to 4D3 c. Remove the two screws that secure
cryoscopes without a suffix: the pump bracket to the cooling
a. Remove the heat transfer fluid from assembly (C).
the front cover. d. Disconnect the hose from the pump
mechanism (D).

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4DP360 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 A 5. Unplug the thermoelectric connector and
the block probe from the main control
board.

6. Lift out the cooling assembly.

7. Unplug the fan connection (E) and

remove the four nuts and washers from
the fan (F).

C B

8. Replace the old fan with the new one and

reassemble the instrument by reversing
steps 1-7.

The following instructions refer to 4D3

cryoscopes without a suffix:
a. Remove the seven screws from the
deck and remove the deck.
b. Disconnect the hose from the pump
mechanism.
c. Remove the wingnut that secures the
two brackets to the chassis, and then
remove the brackets.

4DP360 Rev0
(4D35 Service Manual)
Page 2 of 2

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 Display Board and Harness Replacement
4D3851
Reference: Use this instruction with replace- The following instructions refer to 4D3
ment part 4D3851. cryoscopes without a suffix:
a. Remove the heat transfer fluid from
Tools Needed: Phillips screwdriver, flat-
the front cover.
bladed screwdriver, adjustable
wrench, static grounding b. Remove the two screws from the rear
(earthing) wrist strap (includ- panel of the back cover and lift off the
ed). back cover.
c. Remove the four screws from under-
Warning-Hazardous Voltage neath the front cover, lift the front
cover slightly up and then slide it for-
Warning-Internal components may ward and off.
be damaged by static electricity.
3. Pull the keypad panel down.
CAUTIONS:
Note: If you have a 4D3 cryoscope with no
• Power cord must be disconnected suffix, the keypad does not pull down.
to prevent electric shock. It is mounted to the front half of the
case, with nuts and locking hardware.
• Never unpack, touch or handle any
integrated circuit without wearing
4. Attach the static grounding strap to your
a grounding (earthing) strap to
wrist and stick the adhesive end to a suit-
minimize your static charge.
able bare-metal ground, such as the rear
panel.
Instruction:
5. Disconnect the keypad and display har-
ness assembly from the main circuit
1. Turn off the power and unplug the instru-
board.
ment.
6. Disconnect the keypad and display con-
2. Remove the screws securing the instru-
nectors (A) from the main harness.
ment cover, and remove the cover (see
below):
7. Remove the four screws that attach the
The following instructions refer to 4D3 keypad display board to the keypad (B).
cryoscopes with suffix "A" or later, and
to all 3D3 osmometers: 8. Remove the four screws (C) that attach
the display to the keypad, and remove the
a. Remove the two sets of screws from display.
the side panels and the two screws
from the rear panel. 9. Replace the old display board and har-
b. Slide the instrument cover up and off. ness with the new one and reassemble by
reversing steps 1-8.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4DP851R Rev1
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 C

A
B B

4DP851R Rev1
(4D35 Service Manual)
Page 2 of 2

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 EPROM Replacement
716811R
(4D3 Instruments with Serial Suffix A through E*)

Reference: Use this instruction with replace- To determine if your instrument contains a
ment part 716811R. 256K EPROM main board, remove the cover
and look at the upper left corner of the main
Tools Needed: Phillips screwdriver, flat- board. If your board contains component Q9,
bladed screwdriver, static you still have the 256K EPROM main board
grounding (earthing) wrist and can use this upgrade.
strap (included), 28-pin IC
removal tool (alternatively, a Upon completion of this replacement, the
small screwdriver may be software version reported at power-up will
used with caution so as not to match the version number printed on the new
damage the IC or the socket). EPROM.

Warning-Hazardous Voltage
Instruction:
Warning-Internal components may
1. Turn off the power and unplug the instru-
be damaged by static electricity.
ment.
CAUTIONS:
2. Attach the static grounding strap to your
• Instrument calibration and all cus- wrist and stick the adhesive end to a suit-
tomized settings will be lost when able bare-metal ground.
the EPROM is replaced. Therefore,
make sure the current sample and 3. Remove the screws securing the instru-
block probe bin numbers and the ment cover, and remove the cover:
sample stir amplitude are recorded
in the service log so that you can a. Remove the two sets of screws from
reset these vital numbers into the the side panels and the two screws
replacement EPROM. from the rear panel.

• Never unpack, touch or handle an b. Slide the instrument cover up and off.
EPROM or any other integrated
circuit without wearing a ground- 4. Locate EPROM IC U8 on the circuit
ing (earthing) strap to minimize board. (U8 bears a white label indicating
your static charge. the software version number.) Note the
orientation of the small locating notch in
one corner, and/or locating mark on one
*EPROM Replacement 716811R may be used edge of the IC.
to update the operating software in all
Advanced Model 4D3 cryoscopes with serial 5. Make sure your static draining connection
numbers prior to suffix F that contain a 256K is intact, and then remove IC U8 from the
EPROM main board. circuit board.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 71P811R Rev1
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 6. Observing proper orientation and making 5. Enter each digit of the serial number as it
sure each of the 28 pins enters its proper appears on the label on the back of the
socket, press the new IC U8 firmly into instrument (Note: the letter suffix at the
the sockets. end of the serial number will not be
entered). When the number is correct,
7. Replace the instrument cover and recon- press ENTER.
nect the power cord.
The serial number will be recorded in
8. Turn on the power switch. The instru- memory and should appear the next time
ment will default to the Setup mode until you power up your instrument.
sample and block probe bin numbers are
entered.

9. Reset the sample and block probe bin

numbers and the sample stir amplitude
from the service log entries, and any cus-
tomized settings.

10. Reset the serial number (see the following

procedure).

11. Press STOP to exit the Setup menu. The

instrument will run the self-diagnostics
program.

12. Recalibrate the instrument.

Reset Serial Number

1. If there is a Supervisor/Operator
keyswitch, turn it to the Supervisor posi-
tion (if required).

2. At “Press START to Continue”, press

SETUP.

3. Cycle through the menu options by press-

ing < or > until you reach “Serial
Number”, and then press START. The
display will read “Serial#:********”.

4. Press the following keys consecutively:

2, 4, CLEAR. The displayed serial num-
ber will disappear.

71P811R Rev1
(4D35 Service Manual)
Page 2 of 2

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 EPROM Replacement
716824R
(4D3 Instruments with Serial Suffix F Through T)

Reference: Use this instruction with replace- Instruction:

ment part 716824R.
1. Turn off the power and unplug the instru-
Tools Needed: Phillips screwdriver, flat- ment.
bladed screwdriver, static
grounding (earthing) wrist 2. Attach the static grounding strap to your
strap (included), 28-pin IC wrist and stick the adhesive end to a suit-
removal tool (alternatively, a able bare-metal ground.
small screwdriver may be
used with caution so as not to 3. Remove the screws securing the instru-
damage the IC or the socket). ment cover, and remove the cover:
a. Remove the two sets of screws from
Warning-Hazardous Voltage
the side panels and the two screws
from the rear panel.
Warning-Internal components may
be damaged by static electricity. b. Slide the instrument cover up and off.

CAUTIONS: 4. Locate EPROM IC U8 on the circuit

• Instrument calibration and all cus- board. (U8 bears a white label indicating
tomized settings will be lost when the software version number.) Note the
the EPROM is replaced. Therefore, orientation of the small locating notch in
make sure the current sample and one corner, and/or locating mark on one
block probe bin numbers and the edge of the IC.
sample stir amplitude are recorded
in the service log so that you can 5. Make sure your static draining connection
reset these vital numbers into the is intact, and then remove IC U8 from the
replacement EPROM. circuit board.

• Never unpack, touch or handle an 6. Observing proper orientation and making

EPROM or any other integrated sure each of the 28 pins enters its proper
circuit without wearing a ground- socket, press the new IC U8 firmly into
ing (earthing) strap to minimize the sockets.
your static charge.
7. Replace the instrument cover and recon-
nect the power cord.
Upon completion of this replacement, the
software version reported at power-up will 8. Turn on the power switch. The instru-
match the version number printed on the new ment will default to the Setup mode until
EPROM. sample and block probe bin numbers are
entered.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 71P824R Rev1
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 9. Reset the sample and block probe bin
numbers and the sample stir amplitude
from the service log entries, and any cus-
tomized settings.

10. Reset the serial number (see the following

procedure).

11. Press STOP to exit the Setup menu. The

instrument will run the self-diagnostics
program.

12. Recalibrate the instrument.

Reset Serial Number

1. If there is a Supervisor/Operator
keyswitch, turn it to the Supervisor posi-
tion (if required).

2. At “Press START to Continue”, press

SETUP.

3. Cycle through the menu options by press-

ing < or > until you reach “Serial
Number”, and then press START. The
display will read “Serial#:********”.

4. Press the following keys consecutively:

2, 4, CLEAR. The displayed serial num-
ber will disappear.

5. Enter each digit of the serial number as it

appears on the label on the back of the
instrument (Note: the letter suffix at the
end of the serial number will not be
entered). When the number is correct,
press ENTER.

The serial number will be recorded in

memory and should appear the next time
you power up your instrument.

71P824R Rev1
(4D35 Service Manual)
Page 2 of 2

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 EPROM Replacement
716825R
(3D3 Instruments with Serial Suffix D and Later)

Reference: Use this instruction with replace- Instruction:

ment part 716825R.
1. Turn off the power and unplug the instru-
Tools Needed: Phillips screwdriver, flat- ment.
bladed screwdriver, static
grounding (earthing) wrist 2. Attach the static grounding strap to your
strap (included), 28-pin IC wrist and stick the adhesive end to a suit-
removal tool (alternatively, a able bare-metal ground.
small screwdriver may be
used with caution so as not to 3. Remove the screws securing the instru-
damage the IC or the socket). ment cover, and remove the cover:
a. Remove the two sets of screws from
the side panels and the two screws
Warning-Hazardous Voltage
from the rear panel.
Warning-Internal components may b. Slide the instrument cover up and off.
be damaged by static electricity.
4. Locate EPROM IC U8 on the circuit
CAUTIONS: board. (U8 bears a white label indicating
• Instrument calibration and all cus- the software version number.) Note the
tomized settings will be lost when orientation of the small locating notch in
the EPROM is replaced. Therefore, one corner, and/or locating mark on one
make sure the current sample and edge of the IC.
block probe bin numbers and the
sample stir amplitude are recorded 5. Make sure your static draining connection
in the service log so that you can is intact, and then remove IC U8 from the
reset these vital numbers into the circuit board.
replacement EPROM.
6. Observing proper orientation and making
• Never unpack, touch or handle an sure each of the 28 pins enters its proper
EPROM or any other integrated socket, press the new IC U8 firmly into
circuit without wearing a ground- the sockets.
ing (earthing) strap to minimize
your static charge. 7. Replace the instrument cover and recon-
nect the power cord.
Upon completion of this replacement, the
software version reported at power-up will 8. Turn on the power switch. The instru-
match the version number printed on the new ment will default to the Setup mode until
EPROM. sample and block probe bin numbers are
entered.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 71P825R Rev1
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

Downloaded from www.Manualslib.com manuals search engine

 9. Reset the sample and block probe bin
numbers and the sample stir amplitude
from the service log entries, and any cus-
tomized settings.

10. Reset the serial number (see the following

procedure).

11. Press STOP to exit the Setup menu. The

instrument will run the self-diagnostics
program.

12. Recalibrate the instrument.

Reset Serial Number

1. If there is a Supervisor/Operator
keyswitch, turn it to the Supervisor posi-
tion (if required).

2. At “Press START to Continue”, press

SETUP.

3. Cycle through the menu options by press-

ing < or > until you reach “Serial
Number”, and then press START. The
display will read “Serial#:********”.

4. Press the following keys consecutively:

2, 4, CLEAR. The displayed serial num-
ber will disappear.

5. Enter each digit of the serial number as it

appears on the label on the back of the
instrument (Note: the letter suffix at the
end of the serial number will not be
entered). When the number is correct,
press ENTER.

The serial number will be recorded in

memory and should appear the next time
you power up your instrument.

71P825R Rev1
(4D35 Service Manual)
Page 2 of 2

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 Ferrite Bead Rework Instruction
Reference: Use this instruction to install part 3. Locate the white wire exiting the head
91035. shaft and running to the head transition PC
board.
Tools Needed: Phillips screwdriver, flat-blad-
4. If you see the white wire passing through
ed screwdriver, static ground-
a round black bead(s) as shown in the
ing (earthing) wrist strap
installation option A or B pictures, then
(included).
your instrument already contains this
update and you may disregard this docu-
Warning-Hazardous Voltage
ment.
Warning-Internal components may be 5. If you do not have one of these beads
damaged by static electricity. installed, then please follow the installa-
tion instructions, below.
CAUTIONS:
• Power cord must be disconnected to Upgrade Installation (Option A):
prevent electric shock. 1. Locate the two ferrite beads, Part No.
• Never unpack, touch or handle any 91035, shipped with this kit.
integrated circuit without wearing a
grounding (earthing) strap to mini- 2. Locate the white wire and its connection
mize your static charge. to terminal TR2 on the head transition
board.
Advanced Instruments, Inc. has identified a
condition affecting some instruments, 3. De-solder the wire and slip it through the
described in your service manual as Stir center of the two ferrite beads.
Driver Noise. This electrical noise can be the 4. Re-solder the wire to the TR2 terminal.
cause of false pre-freeze errors, sample freeze
errors, and in some cases may also contribute 5. Check terminal TR2 to verify that it has
to poor repeatability and excessive wear of the not become loose from the printed circuit
stir coil and clapper assembly. board. If necessary, apply some heat and
solder to the back of TR2 to secure it in
It is recommended that this update be added to place.
your 4D3 and 3D3 instruments. All products 6. Use the stir/freeze test and stir/freeze
manufactured starting in September 2003 have alignment instructions contained in your
this modification as standard equipment, user’s guide to verify correct operation.
installed as described in “Factory-installed
Upgrade (Option B)”. 7. Restore the head cover and screws.

Determining Need of Upgrade: Factory-installed Upgrade (Option B):

1. Turn off the instrument power and discon- This option is the standard factory installation.
nect the power cord. These photographs have been provided as ref-
erence only, for determination of the installed
2. Remove the two screws holding the cover upgrade. Field upgrades should use option A,
on the sample head assembly. as described above.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4SP475 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 Option A: Upgrade Installation

Ferrite Beads

NOTE: For installation Option A, locate two ferrite beads on the white wire exiting the head shaft and
attached to TR2 on the head transitionn board.

Option B: Factory-installed Upgrade

Ferrite Bead
Ferrite Bead

NOTE: For installation Option B, locate one ferrite bead

on the light blue wire running between Q2B emit-
ter and J3B. The second ferrite bead is placed on
the white wire exiting the head shaft and attached
to TR2 on the head transition board.

4SP475 Rev0
(4D35 Service Manual)
Page 2 of 2

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 Fuse Replacement
70011/70022
(Instruments with Serial Suffix Prior to P)

Reference: Use this instruction with replace- The following instructions refer to 4D3
ment part 70011 (1.0-Amp) or cryoscopes with suffix before "D", and to
70022 (2.0-Amp). 3D3 osmometers with suffix before “B”:

Voltage/Position/Fuse: 1. Turn off the power and unplug the instru-

100-130 250V 2.0-Amp time delay ment.
200-250 250V 1.0-Amp time delay
2. Insert a flat-bladed screwdriver between
Tools Needed: Flat-bladed screwdriver. the fuse holder and the power entry mod-
ule. Carefully pry the fuse holder from
the power entry module.
Warning-Hazardous Voltage
3. Dislodge the old fuses and reinstall the
new fuses by placing them in the fuse
CAUTION: Improper connections holder brackets.
may cause damage to the instrument.
4. Slide the indicator pin into the correct slot
on the voltage board (A).

5. Place the fuse holder back into the power

entry module, making sure the indicator
pin extends through the correct pin hole
on the fuse holder (B).

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 700P11 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 3

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 The following instructions refer to 4D3
cryoscopes with suffix "D" through “P”,
and to 3D3 osmometers with suffix “B”
through “P”:

1. Turn off the power and unplug the instru-

ment.

2. Insert a flat-blade screwdriver between

the fuse holder and the power entry mod-
ule. Carefully pry the fuse holder from
the power entry module.

3. Dislodge the old fuses (C) and reinstall

the new fuses by placing them in the fuse
holder brackets.

4. Place the fuse holder back into the power

entry module. Note that the arrow (D)
indicating the correct voltage for the out-
let must align with the fuse holder.

700P11 Rev0
(4D35 Service Manual)
Page 2 of 3

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 Fuse Replacement
70011/70022
(Instruments with Serial Suffix P or Greater)

Reference: Use this instruction with replace- 2. Use a flat-bladed screwdriver to pry out
ment part 70011 (1.0-Amp) or the fuse holder door and then the fuse
70022 (2.0-Amp). holder module.

Voltage/Position/Fuse: 3. Dislodge the old fuses and reinstall the

100-130 250V 2.0-Amp time delay new fuses by placing them in the fuse
200-250 250V 1.0-Amp time delay holder brackets.

Tools Needed: Flat-bladed screwdriver. 4. Place the fuse holder back into the instru-
ment, with the correct voltage on the fuse
holder showing in the window (A) on the
Warning-Hazardous Voltage fuse holder socket.

If the AC voltage of the power outlet

CAUTION: Improper connections intended for the instrument is within the
may cause damage to the instrument. 100-130V range, the window should indi-
cate 115V. If the power outlet AC volt-
age is within the 200-250V range, the
Instructions: window should indicate 230V.

1. Turn off the power and unplug the instru-

ment.

700P11 Rev0
(4D35 Service Manual)
Page 3 of 3

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Downloaded from www.Manualslib.com manuals search engine
 Head Up Relay or Head Down Relay Replacement
3D3109
Reference: Use this instruction with replace- c. Remove the four screws from under-
ment part 3D3109. neath the front cover, lift the front
cover slightly up and then slide it for-
Tools Needed: Phillips screwdriver, flat- ward and off.
bladed screwdriver.
3. Locate the Head Up/Head Down Relay on
the inside rear wall. Note the wire colors
Warning-Hazardous Voltage and the corresponding connectors, and
then remove the connectors.
Warning-Internal components may
be damaged by static electricity. 4. Remove the two screws (A) from the
relay and replace the old relay with the
CAUTION: Improper connections new one.
may cause damage to the instrument.
5. Reassemble by reversing steps 1-4.

Instruction:
Head Up Relay
1. Turn off the power and unplug the instru- A
ment.

2. Remove the screws securing the instru-

ment cover, and remove the cover (see Brown Blue
below):
The following instructions refer to 4D3
cryoscopes with suffix "A" or later, and
to all 3D3 osmometers: Yellow Orange
a. Remove the two sets of screws from
the side panels and the two screws
from the rear panel. Brown Blue

b. Slide the instrument cover up and off.

The following instructions refer to 4D3

Gray White
cryoscopes without a suffix:
a. Remove the heat transfer fluid from
the front cover.
b. Remove the two screws from the rear A
panel of the back cover and lift off the
back cover. Head Down Relay

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 3DP109 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1

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Downloaded from www.Manualslib.com manuals search engine
 Heat Transfer Fluid Pump Assembly Replacement
4D3690
Fluid Filter Replacement
4D3710
One-way Check Valve Replacement
4D3705 or 99133R

Reference: Use this instruction with replace- cryoscopes use a closed-loop circulation sys-
ment parts 4D3690, 4D3710, tem for the heat transfer fluid in the cooling
4D3705, or 99133R. system. Various design modifications to the
Pump Assembly affect the part replacement
Tools Needed: Phillips screwdriver, flat- instructions. Please turn to the appropriate
bladed screwdriver. section listed in the table of contents, based
upon the part being replaced and the model
instrument that is being serviced.
Warning-Hazardous Voltage
Instruments shipped prior to 1996 have a
Warning-Internal components may cooling assembly inlet tube with a much
be damaged by static electricity. smaller diameter. A tubing adapter has been
provided in the 4D3690 kit to allow easy
CAUTION: Improper tubing connec- connection to this smaller tube.
tions may result in fluid leakage and
may cause damage to the instrument. Instruments shipped prior to 2004 use the
same type of one-way check valve (4D3705)
in two places. One is located next to the
General Information: Fluid Filter on the pump intake in the Heat
Transfer Fluid bottle. The other is located at
Advanced Instruments, Inc. Models 3D3, the outlet end of the Pump Assembly
4D3, 3250, and 4250, and Fiske Associates attached to the inlet of the Cooling Assembly.
Models Mark 2 and Mark 3 osmometers and Effective in 2004, the one-way check valve

Table of Contents
Replacement Part Model Page

Heat Transfer Fluid Pump Assembly (4D3690) 3D3, Mark 3, 4D3, Mark 2 2
Heat Transfer Fluid Pump Assembly (4D3690) 3250, 4250 4
Fluid Filter (4D3710) All 5
Bottle One-way Check Valve (4D3705) All 5
Cooling System Inlet One-way Check Valve All 6
(99133R)

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4DP690 Rev2
Service Center (U.S. 1-800-225-4034, outside (4D35/3255/MK05 Service Manual)
North America +US 1-781-320-9000). Page 1 of 7

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 Cable Clamp Main Pump
Cooling System Inlet
Check Valve (99133R)

Bottle Check Valve Manual Primer Pump

Fluid Filter

4D3690 Pump Assembly

Figure 1

attached to the Cooling Assembly inlet has 2. Remove the screws securing the instru-
been changed to a different, larger check ment cover, and remove the cover (see
valve (99133R) to improve instrument per- below):
formance and reliability.
The following instructions refer to 4D3
Figure 1 shows the current 4D3690 Pump cryoscopes without a suffix:
Assembly with the various components a. Remove the heat transfer fluid from
labeled. the front cover.

CAUTION: The heat transfer fluid b. Remove the two screws from the rear
contains hazardous chemicals. panel of the back cover and lift off the
Consult the material safety data sheet back cover.
(MSDS) and use appropriate personal c. Remove the four screws from under-
protective equipment. neath the front cover, lift the front
cover slightly up, and then slide it for-
ward and off.
4D3690 Heat Transfer Fluid Pump
Assembly Replacement The following instructions refer to 4D3
Models 3D3 and Mark 3 Osmometers cryoscopes with suffix "A" or later,
Models 4D3 and Mark 2 Cryoscopes and to all 3D3 osmometers:
a. Remove the two sets of screws from
Instruction: the side panels and the two screws
from the rear panel.
1. Turn off the power and unplug the instru-
ment. b. Slide the instrument cover up and off.

4DP690 Rev2
(4D35/3255/MK05 Service Manual)
Page 2 of 7

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 3. Drain the system: 7. Replace the old assembly with the new
one.
a. Remove the heat transfer fluid bottle
and empty the contents. a. If the instrument is a 3D3 or Mark
b. Replace the empty bottle and insert 3 osmometer and the Spacer Strip is
the tubes. not present, remove the green liner
and install the Spacer Strip as shown
c. Press and release gently on the pump in Figure 3. This will ensure that the
(A) at the rear of the cooling assem- proper amount of heat transfer liquid
bly (see Figure 2). Continue until all is pumped during each cycle.
liquid has been expelled.
b. Proceed with the installation of the
4. If present, remove the screw holding the new pump assembly by reversing
cable clamp (B) to the cooling system steps 1-4, making sure to use the
shroud (see Figure 2). Firmly gripping cable clamp already installed on the
the cooling system inlet tube (C), remove new pump assembly. Note the orien-
the check valve, disconnecting the pump tation of the cable clamp (see Figure
from the cooling assembly. 4).
c. Note that just above the heat transfer
5. Remove the pump (A) from the bracket fluid bottle, a small priming pump has
(D). been added to assist with priming and
emptying the pump assembly. This
6. Feed the fluid filter end of the pump should be positioned as shown in
assembly up and around the cooling sys- Figure 6.
tem support brackets, and remove the
pump assembly. d. Make sure that there is no fluid leak-
age from the tubing connections.

Figure 2 Figure 3

4DP690 Rev2
(4D35/3255/MK05 Service Manual)
Page 3 of 7

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 4D3690 Heat Transfer Fluid Pump
Assembly Replacement
Model 3250 Osmometers
Model 4250 Cryoscopes

Instruction:

1. Turn off the power and unplug the instru-

ment.

2. Remove the screws securing the instru-

ment cover, and remove the cover:
a. Remove the two screws from the
front of the cover and the four screws
from the rear panel.
Orient check
b. Lift and tilt the cover so that the rec- valve with arrow,
tangular opening clears the front of as shown
the head cover first, then move the
instrument cover back, up and off.
Figure 4
3. Drain the system:
a. Remove the heat transfer fluid bottle
and empty the contents.
b. Replace the empty bottle and insert
the tubes.
c. Press and release gently on the pump
(E) at the rear of the cooling assembly
(see Figure 5). Continue until all liq-
uid has been expelled.

4. Remove the screw holding the cable

clamp (F) to the cooling system shroud
(see Figure 5). Firmly gripping the cool-
ing system inlet tube (G), remove the
check valve, disconnecting the pump
from the cooling assembly.

5. Remove the pump (E) from the bracket

(H).
E H F G
6. Feed the fluid filter end of the pump
assembly up and around the cooling sys-
tem support brackets, and remove the Figure 5
pump assembly.

4DP690 Rev2
(4D35/3255/MK05 Service Manual)
Page 4 of 7

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 7. Replace the old assembly with the new
one.

a. Proceed with the installation of the

new pump assembly by reversing steps
1-4, making sure to use the cable
clamp already installed on the new
pump assembly. Note the orientation
of the cable clamp (see Figure 4).
b. Make sure that there is no leakage
from the tubing connections.
I

4D3710 Fluid Filter Replacement

Note: The instrument cover does not need to

be removed to replace this part.

Instruction:

1. Open the heat transfer fluid compartment

door.
Figure 6
2. Remove the tube with filter attached from
the bottle of heat transfer fluid (Figure 6).
Use a paper towel or absorbent cloth to 2. Remove the tube with filter attached
soak up excess heat transfer fluid. from the bottle of heat transfer fluid
(Figure 6). Use a paper towel or
3. Unscrew the old filter (I) from the pump absorbent cloth to soak up excess heat
tubing. transfer fluid.

4. Replace the old filter with the new one, 3. Holding the tube in an elevated position
and then replace the tube into the heat to minimize leakage, firmly grip the tube
transfer fluid bottle. just above the old check valve, then pull
off the old check valve and filter.

4. Immediately insert the new check valve,

4D3705 Bottle One-way Check making sure to orient the check valve
Valve Replacement properly (Figure 7).

Note: The instrument cover does not need to 5. Remove the old check valve from the
be removed to replace this part. short piece of tube that connects it to the
filter, then reattach the tube and filter to
Instruction: the new check valve. Replace the tube
with filter into the heat transfer fluid
1. Open the heat transfer fluid compartment bottle.
door.

4DP690 Rev2
(4D35/3255/MK05 Service Manual)
Page 5 of 7

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 99133R Cooling System Inlet One-
way Check Valve Replacement

Instruction:
1. Turn off the power and unplug the instru-
ment.

2. Remove the screws securing the instru-

ment cover, and remove the cover (see
Fluid
below):
Flow
The following instructions refer to 4D3
cryoscopes without a suffix:
a. Remove the heat transfer fluid from
the front cover.
b. Remove the two screws from the rear
panel of the back cover and lift off the
back cover.
c. Remove the four screws from under- Fluid Filter
neath the front cover, lift the front
cover slightly up, and then slide it for-
ward and off. Figure 7

The following instructions refer to 4D3

cryoscopes with suffix "A" or later, cooling system shroud (see Figure 2 or
and to all 3D3 osmometers: Figure 5). Firmly gripping the pump
assembly outlet tube, remove the check
a. Remove the two sets of screws from valve.
the side panels and the two screws
from the rear panel. 4. If needed, slip the new cable clamp onto
b. Slide the instrument cover up and off. the pump assembly outlet tube.

The following instructions refer to 5. Firmly grip the cooling assembly inlet
4250 cryoscopes and 3250 osmometers: tube and remove the check valve.
Discard the old check valve and cable
a. Remove the two sets of screws from clamp.
the side panels and the four screws
from the rear panel. 6. Install the new check valve, making sure
b. Lift and tilt the cover so that the rec- to orient the check valve properly (see
tangular opening clears the front of Figure 4). Connect the new check valve
the head cover first, then move the to the cooling system inlet tube, then fas-
instrument cover back, up and off. ten the new cable clamp using the hard-
ware saved in step 3.
3. If present, remove and save the screw On instruments shipped prior to 1996, the
holding the cable clamp (B or F) to the cooling assembly inlet tube was a much

4DP690 Rev2
(4D35/3255/MK05 Service Manual)
Page 6 of 7

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 smaller diameter. A tubing adapter has
been provided in the 99133R kit to allow
easy connection to this smaller tube.

7. With the heat transfer pump assembly

inlet and outlet tubes inserted into a bottle
of heat transfer fluid, gently press the
pump a few times to purge any trapped
air and to check the proper functioning of
the heat transfer pump assembly.
Make sure that there is no fluid leakage
from the tubing connections.

8. Replace the instrument cover.

4DP690 Rev2
(4D35/3255/MK05 Service Manual)
Page 7 of 7

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 ISO 5764 Upgrade Kit
4D3101
(4D3 Instruments with Serial Suffix G through T)

Contents: Instruction:
1 4D3102 Non-Metallic Sample Probe Kit
1 716824R Model 4D3 Software Upgrade 1. Replace the sample probe according to
Kit the instructions contained in the 4D3102
Sample Probe Replacement kit.

Overview: 2. Upgrade the cryoscope firmware accord-

ing to the instructions contained in the
This kit provides the materials necessary to 716824R EPROM Replacement kit.
upgrade an Advanced Instruments Model 4D3
Cryoscope, serial suffix G-T, for compliance 3. Configure the Model 4D3 to the new ISO
with the requirements of the new ISO 5764: 5764 standard by selecting Setup menu
Milk -- Determination of Freezing Point stan- item #10, “Select Plateau Mode”, and
dard. then selecting ISO 5764. (See Changing
operating settings in the User’s Guide.)
The new ISO 5764 plateau-seeking method
affects two elements of the Model 4D3
cryoscope:

• The software used to control the process

and determine the final reading has been
updated to comply with the require-
ments of the standard.
• The sample probe is now manufactured
with a non-metallic shaft.

NOTE: To comply with the new ISO 5764

standard, both the new non-metallic
probe and the new software must be
used.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4D3P01 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1

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 ISO 5764 Upgrade Kit
4D3104
(4D3 Instruments with Serial Suffix U and Later)

Contents: Instruction:
1 4D3102 Non-Metallic Sample Probe Kit
1 4D3990 Model 4D3 Software Upgrade 1. Replace the sample probe according to
Kit: Disk and Cable the instructions contained in the 4D3102
Sample Probe Replacement kit.

Overview: 2. Upgrade the cryoscope firmware accord-

ing to the instructions contained in the
This kit provides the materials necessary to 4D3990 kit.
upgrade an Advanced Instruments Model 4D3
Cryoscope, serial suffix U, for compliance 3. Configure the Model 4D3 to the new ISO
with the requirements of the new ISO 5764: 5764 standard by selecting Setup menu
Milk -- Determination of Freezing Point stan- item #10, “Select Plateau Mode”, and
dard. then selecting ISO 5764. (See Changing
operating settings in the User’s Guide.)
The new ISO 5764 plateau-seeking method
affects two elements of the Model 4D3
cryoscope:

• The software used to control the process

and determine the final reading has been
updated to comply with the require-
ments of the standard.
• The sample probe is now manufactured
with a non-metallic shaft.

NOTE: To comply with the new ISO 5764

standard, both the new non-metallic
probe and the new software must be
used.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4D3P04 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1

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 Keypad Replacement
3D3511/4D3511
Reference: Use this instruction with replace- c. Remove the four screws from under-
ment part 3D3511 or 4D3511. neath the front cover, lift the front
cover slightly up and then slide it for-
Tools Needed: Phillips screwdriver, flat- ward and off.
bladed screwdriver, adjustable
wrench. 3. Pull the keypad panel down.
Note: If you have a 4D3 cryoscope with no
Warning-Hazardous Voltage suffix, the keypad does not pull down.
It is mounted to the front half of the
Warning-Internal components may case, with nuts and locking hardware.
be damaged by static electricity.
4. Disconnect the keypad and display con-
CAUTION: Improper connections nectors (A) from the main harness.
may cause damage to the instrument.
5. Remove the four screws that attach the
keypad to the keypad assembly (B).
Instruction:
6. Remove the four screws (C) that attach
1. Turn off the power and unplug the instru-
the display to the keypad, and remove the
ment.
display.
2. Remove the screws securing the instru-
7. Replace the old keypad with the new one
ment cover, and remove the cover (see
and reassemble by reversing steps 1-6.
below):
The following instructions refer to 4D3
cryoscopes with suffix "A" or later, and C
to all 3D3 osmometers:
a. Remove the two sets of screws from
the side panels and the two screws
from the rear panel.
b. Slide the instrument cover up and off.

The following instructions refer to 4D3 B

cryoscopes without a suffix: B A

a. Remove the heat transfer fluid from

the front cover.
b. Remove the two screws from the rear
panel of the back cover and lift off the
back cover.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4DP511 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1

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 Load Resistor Replacement Instruction
(Models 4D3, 3D3, Mark 2, Mark 3, 3250, and 4250)

Reference: Use this instruction to install part 2. Remove the instrument cover.
425150R.
3. Pull and drop the keypad and display for-
ward.
For Use On Models:
4D3 with Serial Suffix N or later 4. Locate two #2 holes with threaded inserts
3D3 with Serial Suffix L or later just to the left of the power supply termi-
MK2 with Serial Suffix G or later nal block and install the 425150 load
MK3 with Serial Suffix E or later resistor using provided #2x3/8 screws (A),
3250 -- All as shown in photo.
4250 -- All
5. Disconnect cooling assembly red and
Tools Needed: Small Phillips screwdriver. black wired connector from the M20150
harness.
Warning-Hazardous Voltage 6. Plug both connectors into the mating con-
nector of the load resistor assembly.
Warning-Internal components may
be damaged by static electricity. 7. Replace the instrument cover and restore
power.
8. Verify correct operation using the “A/D
Instruction: Test” in TEST utilities.

1. Turn off the instrument power and discon-

nect the power cord.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 42P150R Rev0
Service Center (U.S. 1-800-225-4034, outside North (4D35/MK05/3255 Service Manuals)
America +US 1-781-320-9000). Page 1 of 1

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 Mandrel Replacement
3LH500

Reference: Use this instruction with replace-

ment part 3LH500. B

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver, 1/16”
Allen wrench, probe align-
ment tool and instructions
(included).
C

CAUTION: Improper connections may

cause damage to the instrument.

6. Seat the mandrel firmly up into the man-

Instruction: drel hole, with the stir/freeze wire channel
perpendicular to the front edge of the
1. Turn off the power and unplug the instru- chassis and no gaps between the flange
ment. and the underside of the chassis.
A
7. Without overtightening (overtightening
can damage the mandrel), re-tighten the
two mandrel setscrews to hold the man-
drel in place.

8. Use the probe alignment tool and instruc-

tions to properly position the probe and
stir/freeze wire.

9. When the probe is in the position

2. Remove the two screws from the head required, tighten the probe setscrew just
cover (A) and lift off the head cover. enough to firmly hold the probe, but not
crack or distort the thin plastic wall of the
3. Loosen the two mandrel setscrews (B) mandrel.
and the probe setscrew (C).
10. Arrange the probe leads so that they will
4. Hold the probe gently while pulling the not touch the clapper or be pinched by the
mandrel down past the probe and off. head cover, and replace the cover.

5. Carefully, push the new mandrel up past 11. Plug in the power cord and return the
the probe and into position on the flange. instrument to operation.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 3LHP500 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1

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 Motor Assembly Replacement
4D3350
Reference: Use this instruction with replace- b. Remove the two screws from the rear
ment part 4D3350. panel of the back cover and lift off the
back cover.
Tools Needed: Phillips screwdriver, flat-
c. Remove the four screws from under-
bladed screwdriver, 1/16”
neath the front cover, lift the front
Allen wrench
cover slightly up and then slide it for-
ward and off.
Warning-Hazardous Voltage
3. Disconnect the three motor connectors
from the harness.
Warning-Internal components may
be damaged by static electricity.
4. Remove the six screws from the deck (A),
and remove the deck.
CAUTION: Improper connections
may cause damage to the instrument.
Note: If you have a 4D3 cryoscope with no
suffix, there are seven screws on the
deck that you must remove.
Instruction:

1. Turn off the power and unplug the instru- A

ment. B

2. Remove the screws securing the instru-

ment cover, and remove the cover (see
below):
The following instructions refer to 4D3
cryoscopes with suffix "A" or later, and
to all 3D3 osmometers:
a. Remove the two sets of screws from
the side panels and the two screws
5. Remove the deck and motor assembly and
from the rear panel.
place it on a flat surface near the instru-
b. Slide the instrument cover up and off. ment.

The following instructions refer to 4D3 6. Remove the two screws (B) that secure
cryoscopes without a suffix: the bracket to the deck.

a. Remove the heat transfer fluid from 7. Remove the two Allen (C) screws that
the front cover. secure the clutch to the motor shaft. If

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4DP350 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 C

necessary, use a screwdriver to gently pry

the clutch from the shaft.

8. Remove the four screws (D) that secure

the motor to the bracket, and remove the
motor.

9. Replace the old motor with the new one

and reassemble by reversing steps 1-8.

4DP350 Rev0
(4D35 Service Manual)
Page 2 of 2

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 NVRAM Battery Replacement
71027R
Reference: Use this instruction with: Instruction:
Advanced Instruments Inc.
4250 Cryoscopes 1. Turn off the instrument power and remove
3250 Osmometers the power cord from the rear of the instru-
3320 Osmometers ment.
2020 Osmometers
4-digit year Model 3300 Osmometers 2. Open the instrument cover.
Intel-based 3D3 Osmometers
Intel-based 4D3 Cryoscopes 3. Attach the static grounding strap accord-
Fiske Associates ing to the instructions on its packaging.
210 Osmometers
Intel-based MK-3 Osmometers 4. Locate the processor Printed Circuit Board
Intel-based MK-2 Cryoscopes (PCB120, PCB125, PCB620, 200016PC,
or 210102PC) and the NVRAM chip:
Tools Needed: Small flat-bladed screwdriver,
• If using PCB120, locate U14.
static grounding (earthing)
wrist strap (included). • If using PCB125 locate U13.

CAUTION: Unplug the power cord • If using PCB620, remove the processor
prior to opening or removing covers, board from the application board and
or else you may be exposed to electric locate U15 on the back side.
shock, excessive temperatures, or • If using 200016PC, locate U11.
mechanical hazards.
• If using 210102PC, locate U11.
Performing this service or maintenance
should only be done by a qualified 5. Once you have located the NVRAM chip,
service technician. locate the small notch and insert the small
screwdriver, as described in figure 1.
A discharge of static electricity from
contact with the human body or other
conductor may damage system boards
or static sensitive devices. Never per-
form internal maintenance without fol-
lowing recommended static protection
procedures.

Figure 1

71P27R Rev3
For additional information or technical assistance, please
(2025/2105/3255/3305/3325/
contact Advanced Instruments Hot-Line® Service Center.
(U.S. 1-800-225-4034, outside North America +US 1-781- 4D35/MK05 Service Manuals)
320-9000. After normal business hours, dial extension 2191.) Page 1 of 2

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 6. Remove the cover (powercap) and set it 10. Set the date and time, following the
aside. instructions in the user’s guide.

7. Remove the replacement powercap from 11. Turn off the power and wait ten minutes.
the anti-static bag and install it on the Turn the instrument back on and verify
component base, as described in figure 2. that the correct time has been maintained.

8. Reinstall the processor board, if removed. 12. Turn off the power and restore the instru-
ment cover.
9. Restore power to the instrument and veri-
fy correct power-up sequence without any 13. Reset all stored parameters (e.g., block
LOW BATTERY warnings. and sample bin numbers, plateau modes,
etc.) and recalibrate the instrument.

Figure 2

71P27R Rev3
(2025/2105/3255/3305/3325/
4D35/MK05 Service Manuals)
Page 2 of 2

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 PCB520 Transistor Replacement Instruction
35010 to PCB520R Substitution
(Models 4D3, 3D3, 3250*, 4C3, 3900, and 4250*)

*Not for use on Models 3250/4250 with 5. Remove the screws securing the protec-
serial suffix D and higher. tive black plastic cap on the outside rear
of the instrument.
Reference: Use this instruction to install part
6. Remove the old transistor and insulating
PCB520R, which is intended as a
materials by gently prying with a flat-
direct replacement for part 35010
bladed screwdriver.
metal can FET transistor used on
some of the above models. 7. Remove the transistor socket Q1B from
the rear panel and clear the mounting
Materials Included: holes of any remaining hardware.
1 - PCB520 assembly
2 - H50606, #6x3/8 Sems 8. Cut transistor socket Q1B from harness
2 - H50608, #6x1/2 Sems M20150.
2 - H63093, #6 large flat washers 9. Strip wire insulation ¼” on four (4) wires
2 - 90049, Hex swage standoffs formerly soldered to the transistor socket.
2 - 91038, ¼” plastic hole plugs Twist the stripped ends to prevent fraying.

Tools Needed: Wire cutters, wire strippers, 10. Install the hex standoffs to PCB520 using
small flat-bladed screwdriver, #6x3/8 screws, with the flat end of the
medium Phillips screwdriver. standoff against the board.
11. Connect white/red wire to terminal 1 (A).
Warning-Hazardous Voltage
12. Connect white/brown wire to terminal 2
Warning-Internal components may be (B).
damaged by static electricity. 13. Connect brown wire to terminal 3 (C).
14. Connect black wire to terminal 4 (D).
4D3, 3D3, 3250, and 4250 Instruction:
1. Turn off the instrument power and discon-
nect the power cord.
2. Remove the intrument cover. A
3. Locate transistor Q1B on left-rear of the
B
chassis. Q1B is the transistor nearest to
the top of the rear panel.
C
4. If your instrument already has PCB520 in
this location, remove the old PCB and D
skip to step 11.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® PCP520R Rev4
Service Center (U.S. 1-800-225-4034, outside North (4D35/4C35/3255/3905 Service Manual)
America +US 1-781-320-9000). Page 1 of 3

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 Note: Make sure screw terminals are tight 8. Remove the transistor socket Q3 from the
and that only bare wire, not insulation, heat sink and clear the mounting holes of
is captured in the clamp. any remaining hardware.
15. Install completed assembly inside the 9. Cut transistor socket Q3 from harness
unit, against the rear wall with the termi- 4C3160.
nal block (E) facing the left side. Use
10. Strip wire insulation ¼” on four (4) wires
#6x1/2 screws and large flat washers to
formerly soldered to the transistor socket.
secure swaged end of standoffs to the rear
Twist the stripped ends to prevent fray-
wall, using the old transistor mounting
ing.
holes.
11. Install the hex standoffs to PCB520 using
Note: Newer instruments manufactured in
#6x3/8 screws, with the flat end of the
2005 may have a modified mounting
standoff against the board.
(e.g., terminal block facing down-
ward). See figures on next page for 12. Connect red/white wire to terminal 1 (G).
mounting configurations.
13. Connect white/brown wire to terminal 2
16. Plug the old transistor lead holes, if any, (H).
using the ¼” plastic hole plugs (F).
14. Connect white/green wire to terminal 3
17. Replace the instrument cover and connect (I).
the power cord.
15. Connect black wire to terminal 4 (J).
18. Turn on the instrument and verify correct
16. Install completed assembly on the back
thermoelectric operation using the test
side of the heat sink where the previous
utility “A/D Test” (see user’s guide).
transistor socket was mounted. Use
#6x1/2 screws and large flat washers to
4C3 and 3900 Instruction:
secure swaged end of standoffs to the
1. Turn off the instrument power and dis- heat sink, using the old transistor mount-
connect the power cord. ing holes. The ¼” plastic hole plugs are
not used in this installation and may be
2. Remove the intrument rear cover.
discarded.
3. Locate transistor Q3 on the transistor heat
17. Replace the instrument cover and connect
sink mounted to the bottom of the chas-
the power cord.
sis. Q3 is the transistor 2nd from the left.
18. Turn on the instrument and verify correct
4. If your instrument already has PCB520 in
thermoelectric operation using the test
this location, remove the old PCB and
utility “A/D Test” (see user’s guide).
skip to step 12.
5. Remove the screws securing the heat sink
to the chassis and gently lift out the heat
sink.
6. Remove the screws holding the old tran-
sistor and insulating materials.
7. Remove the old transistor and insulating
materials by gently prying with a flat-
bladed screwdriver.

PCP520R Rev4
(4D35/4C35/3255/3905 Service Manual)
Page 2 of 3

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 F
Alternate Mounting Configuration
(see below)

G
H
I
J

PCP520R Rev4
(4D35/4C35/3255/3905 Service Manual)
Page 3 of 3

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Downloaded from www.Manualslib.com manuals search engine
 PCB522 Transistor Replacement Instruction
35050 to PCB522R Substitution
(Models 4D3, 3D3, 3250*, 4C3, 3900, and 4250*)

*Not for use on Models 3250/4250 with 5. Remove the screws securing the protec-
serial suffix D and higher. tive black plastic cap on the outside rear
of the instrument.
Reference: Use this instruction to install
part PCB522R, which is intend- 6. Remove the old transistor and insulating
ed as a direct replacement for materials by gently prying with a flat-
part 35050 metal can Darlington bladed screwdriver.
transistor used on some of the 7. Remove the transistor socket Q2B from
above models. the rear panel and clear the mounting
holes of any remaining hardware.
Materials Included:
1 - PCB520 assembly 8. Cut transistor socket Q2B from harness
2 - H50606, #6x3/8 Sems M20150.
2 - H50608, #6x1/2 Sems 9. Strip wire insulation ¼” on four (4) wires
2 - H63093, #6 large flat washers formerly soldered to the transistor socket.
2 - 90049, Hex swage standoffs Twist the stripped ends to prevent fraying.
2 - 91038, ¼” plastic hole plugs
10. Install the hex standoffs to PCB522 using
Tools Needed: Wire cutters, wire strippers, #6x3/8 screws, with the flat end of the
small flat-bladed screwdriver, standoff against the board.
medium Phillips screwdriver.
11. Connect white/blue wire to terminal 1
(A).
Warning-Hazardous Voltage
12. Connect violet wire to terminal 2 (B).
Warning-Internal components may
13. Terminal 3 (C) is unused.
be damaged by static electricity.

4D3, 3D3, 3250, and 4250 Instruction:

1. Turn off the instrument power and dis-
connect the power cord. A
2. Remove the intrument cover.
B
3. Locate transistor Q2B on left-rear of the
chassis. Q2B is the transistor nearest to C
the bottom of the rear panel.
4. If your instrument already has PCB522 in D
this location, remove the old PCB and
skip to step 11.
E

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® PCP522R Rev3
Service Center (U.S. 1-800-225-4034, outside North (4D35/4C35/3255/3905 Service Manual)
America +US 1-781-320-9000). Page 1 of 3

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 14. Connect dark blue wire to terminal 4 (D). 7. Remove the old transistor and insulating
materials by gently prying with a flat-
15. Connect light blue wire to terminal 4 (D).
bladed screwdriver.
Note: Make sure screw terminals are tight
8. Remove the transistor socket Q2 from the
and that only bare wire, not insulation,
heat sink and clear the mounting holes of
is captured in the clamp.
any remaining hardware.
16. Install completed assembly inside the
9. Cut transistor socket Q2 from harness
unit, against the rear wall with the termi-
4C3160.
nal block (E) facing the left side. Use
#6x1/2 screws and large flat washers to 10. Strip wire insulation ¼” on four (4) wires
secure swaged end of standoffs to the rear formerly soldered to the transistor socket.
wall, using the old transistor mounting Twist the stripped ends to prevent fray-
holes. ing.
Note: Newer instruments manufactured in 11. Install the hex standoffs to PCB522 using
2005 may have a modified mounting #6x3/8 screws, with the flat end of the
(e.g., terminal block facing down- standoff against the board.
ward). See figures on next page for
mounting configurations. 12. Connect white/blue wire to terminal 1
(G).
17. Plug the old transistor lead holes, if any,
using the ¼” plastic hole plugs (F). 13. Connect violet/white wire to terminal 2
(H).
18. Replace the instrument cover and connect
the power cord. 14. Terminal 3 (I) is unused.

19. Turn on the instrument and verify correct 15. Connect blue/red wire to terminal 4 (J).
stir/freeze operation using the test utility 16. Connect blue wire to terminal 4 (J).
“Stir/Freeze Test” (see user’s guide).
17. Install completed assembly on the back
4C3 and 3900 Instruction: side of the heat sink where the previous
transistor socket was mounted. Use
1. Turn off the instrument power and dis- #6x1/2 screws and large flat washers to
connect the power cord. secure swaged end of standoffs to the
2. Remove the intrument rear cover. heat sink, using the old transistor mount-
ing holes. The ¼” plastic hole plugs are
3. Locate transistor Q2 on the transistor heat not used in this installation and may be
sink mounted to the bottom of the chas- discarded.
sis. Q2 is the transistor 2nd from the
right. 18. Replace the instrument cover and connect
the power cord.
4. If your instrument already has PCB522 in
this location, remove the old PCB and 19. Turn on the instrument and verify correct
skip to step 12. stir/freeze operation using the test utility
“Stir/Freeze Test” (see user’s guide).
5. Remove the screws securing the heat sink
to the chassis and gently lift out the heat
sink.
6. Remove the screws holding the old tran-
sistor and insulating materials.

PCP522R Rev3
(4D35/4C35/3255/3905 Service Manual)
Page 2 of 3

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 F
Alternate Mounting Configuration
(see below)

G
H
I
J

PCP522R Rev3
(4D35/4C35/3255/3905 Service Manual)
Page 3 of 3

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Downloaded from www.Manualslib.com manuals search engine
 Power Supply Assembly Replacement
4D3950/325950R
Reference: Use this instruction with replace- 5. Identify and disconnect the wiring har-
ment part 4D3950 or 325950R. ness connections from the power supply
terminal strip. Note the wire colors and
Use 4D3950 with Models 3250/4250 Serial the corresponding connectors.
Suffix A - C, 3D3, 4D3, Mark 2, or Mark 3.
6. Remove the four screws and nylon wash-
Use 325950R with Models 3250/4250 Serial ers from the power supply board.
Suffix D or higher.
7. Replace the old power supply with the
Tools Needed: Phillips screwdriver, flat- new one and reassemble by reversing
bladed screwdriver, static steps 1-6.
grounding (earthing) wrist
strap (included).

red
Warning-Hazardous Voltage
black
Warning-Internal components may black
be damaged by static electricity.
violet
CAUTION: Improper connections yellow
may cause damage to the instrument.
orange
green/yellow
4D3950 Instruction:
blue/white
1. Turn off the power and unplug the instru- brown/white
ment.

2. Remove the screws securing the head

cover, and remove the cover.

3. Remove the screws securing the instru- 325950R Instruction:

ment cover, and remove the cover.
1. Turn off the power and unplug the instru-
4. The power supply is mounted on the ver- ment.
tical mounting panel behind the main
printed circuit boards. Remove the two CAUTION: IT IS CRITICAL THAT THE
rear screws and the two bottom screws INSTRUMENT BE UNPOW-
securing the power supply mounting ERED THROUGHOUT THIS
panel and lift the entire assembly out far REPLACEMENT PROCE-
enough to access the power supply. DURE. ALWAYS UNPLUG
THE INSTRUMENT FOR
PERSONAL PROTECTION

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 325P950 Rev2
(4D35/3255/MK05 Service Manual)
Service Center (U.S. 1-800-225-4034, outside
Page 1 of 3
North America +US 1-781-320-9000).

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 FROM HAZARDOUS VOLT- C Deck Printer Cover
AGES AND TO PREVENT
POTENTIAL DAMAGE TO
THE INSTRUMENT.

2. Remove the screws securing the head

cover, and remove the cover.

3. Release the printer cover. Remove the

screws securing the instrument cover, then
slide the cover forward, then up and off to
remove. Tilt the keypad forward to pro-
vide access and clearance to the printer
Keypad Main PCB
assembly. Remove the installed paper roll
from the paper assembly.

4. Locate and remove the printer assembly D

mounted between the deck and the main
PCB support to provide access to the
d. Release the main PCB from the stand-
power supply.
offs (D). Some instruments may
require two screws be removed. Tilt
a. Attach the static grounding strap to
the main PCB away from the mount-
your wrist and stick the adhesive end to
ing bracket. Note the front-to-back
a suitable bare-metal ground, such as
position of the mounting screws (E)
the rear panel.
that attach the printer bracket to the
main PCB mounting bracket to assist
b. Release cable end latch and disconnect
during reassembly, then remove the
the printer data cable (A) attached at
two mounting screws.
the lower rear of the printer assembly,
then disconnect the printer power con-
e. To remove the printer assembly, slide
nection (B) located in the middle side
the printer assembly towards the front
of the printer interface PCB, towards
of the instrument until the printer
the sample cooling assembly and head.
bracket is clear of the deck.
c. Remove the two mounting screws (C)
that attach the printer bracket to the
deck.

A B E

325P950 Rev2
(4D35/3255/MK05 Service Manual)
Page 2 of 3

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 7. Install the replacement power supply on
the mounting bracket, making sure to
maintain the same orientation, with the
connections toward the rear of the instru-
ment. Reinstall the power supply/main
F PCB mounting bracket, if necessary.

8. Connect the main harness DC power con-

nector (the larger of the two connectors)
G to the new power supply, then connect the
AC power harness.

9. Reinstall the printer assembly into the

instrument by guiding it back into posi-
tion from the front of the instrument.
Replace the printer bracket mounting
screws, making sure to position the print-
5. Disconnect the AC power harness (F) and er bracket as noted during the disassem-
the main harness DC power connector (G) bly, earlier. This location is important to
from the power supply. ensure proper alignment to the instrument
cover.
6. Remove the power supply by removing the
four (4) mounting screws (H) located at the 10. Remount the main PCB on the mounting
corners of the power supply. standoffs, taking care not to bend or
stress the main PCB. Reinstall two
Note: If necessary, the power supply/main screws, if applicable.
PCB mounting bracket can be removed
by removing the two mounting screws 11. Connect the printer power connection to
from the rear of the instrument, and the the connector on the bottom of the printer
two mounting screws located in the bot- interface board, then connect the printer
tom flange of the mounting bracket at data cable to the connection at the lower
either end. Carefully tilt the bracket to rear of the printer assembly.
access the power supply, taking care not
to damage the main PCB located on the 12. Replace the instrument cover, taking care
other side of the bracket. to align the cover so that there is space on
either side of the printer cover, then
secure with the screws.

13. Replace the head cover, then secure with

the screws.

14. Reinstall the printer paper roll per the

instructions in the User’s Guide.

325P950 Rev2
(4D35/3255/MK05 Service Manual)
Page 3 of 3

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 Power Supply Assembly Replacement
4D3950
Reference: Use this instruction with replace- The following instructions refer to 4D3
ment part 4D3950. cryoscopes with suffix "A" or later, and
to all 3D3 osmometers:
Tools Needed: Phillips screwdriver, flat-
a. Remove the two sets of screws from
bladed screwdriver.
the side panels and the two screws
from the rear panel.
Warning-Hazardous Voltage b. Slide the instrument cover up and off.

Warning-Internal components may The following instructions refer to 4D3

be damaged by static electricity. cryoscopes without a suffix:

CAUTION: Improper connections a. Remove the heat transfer fluid from

may cause damage to the instrument. the front cover.
b. Remove the two screws from the rear
panel of the back cover and lift off the
Instruction: back cover.

1. Turn off the power and unplug the instru- c. Remove the four screws from under-
ment. neath the front cover, lift the front
cover slightly up and then slide it for-
2. Remove the screws securing the instru- ward and off.
ment cover, and remove the cover (see
below):

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4DP950 Rev2
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 3. Hitachi Processor (3D3600/4D3600) 7. Replace the old power supply with the
Only: Check for the presence of IC U36 new one and reassemble by reversing
(A), near the upper front corner of the steps 1-6.
main printed circuit board. Check
whether pin 3 of U36 has been severed
from the main printed circuit board, and a
small busswire jumper soldered between
pins 3 and 2.

If this modification has not already been

accomplished, please do so at this time,
being careful not to overheat U36 with
the soldering iron.

4. The power supply is mounted on the ver-

tical mounting panel behind the main
printed circuit board. Remove the two
rear screws and the two bottom screws
securing the power supply mounting
panel and lift the entire assembly out far
enough to access the power supply.

5. Identify and disconnect the wiring har-

ness connections from the power supply
terminal strip. Note the wire colors and
the corresponding connectors.

6. Remove the four screws and nylon wash-

ers from the power supply board.

red
black
black
violet
yellow
orange
green/yellow
blue/white
brown/white

4DP950 Rev2
(4D35 Service Manual)
Page 2 of 2

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 Probe Alignment Instructions for Alignment Tool
3LA702 or Alignment Toolkit 3LA700
Reference: Use this instruction with part Proper alignment and adjustment of the man-
3LA702 or kit 3LA700 and any drel, probe and stir/freeze wire are of utmost
of the following models of importance to assure accuracy, precision and
Advanced Instruments, Inc. or proper operation of the instrument. Use the
Fiske Associates® osmometers or probe alignment tool to precisely center the
cryoscopes: probe and position the stir wire according to
Advanced Instruments Inc. factory specifications.
4250, 4D3 & 4C3 Cryoscopes
3250, 3D3 & 3900 Osmometers Figure 1 shows all of the components of the
sample head. Although an osmometer head is
Fiske Associates shown, a cryoscope head is almost identical,
Mark 2 Cryoscopes with the only differences being the material of
Mark 3 Osmometers the sample probe and the diameter and shape
of the stir wire.
Tools Needed: Phillips or flat-bladed screw- The alignment and adjustment of the mandrel,
driver; small flat-bladed sample probe, and stir/freeze wire on the
screwdriver; 5/64”, 9/64” and Model 3900 multi-sample osmometer and
1/16” Allen wrenches.

Sample Probe Connector

Clapper

Stir Wire Setscrews Coil

Head

Mandrel Setscrews
Mandrel

Sample Probe Setscrew

Stir Wire Slot in Mandrel

Sample Probe
Stir Wire

Figure 1. Osmometer Sample Head

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® Service 3LP700 Rev10
Center. (U.S. 1-800-225-4034, outside North America (4C35/4D35/3255/MK05 Service Manual)
+US 1-781-320-9000. After normal business hours, dial Page 1 of 5
extension 2191.)

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 Model 4C3 multi-sample cryoscope is essen- Osmometer Instructions:
tially the same as what is described here for
the single-sample osmometer or cryoscope. 1. Facing the instrument from the front,
slide the alignment tool squarely up onto
CAUTION: Turn off the osmometer or the mandrel, round-end first, with the
cryoscope while making the fol- stir/freeze wire located as shown in
lowing checks or adjustments. Figure 3.

General Instructions: 2. Check the probe alignment by making

sure that the probe shaft is lined up along
1. Remove the head cover. the raised V centerline of the alignment
tool and the probe level by making sure
2. Before checking the probe or stir wire that the end of the probe rests on the hori-
alignment, make sure the mandrel is posi- zontal surface below the raised V center-
tioned and aligned, as follows (refer to line, as shown in Figure 3.
Figures 1 and 2):
3. If necessary, make probe centering adjust-
a. Make sure there is no gap between
ments. Bend the metal probe stem gently
the top of the mandrel flange and the
by hand, as necessary, to align the probe
bottom of the sample head.
along the raised V centerline of the probe
b. Look to see if the stir wire slot in the alignment tool.
mandrel is aligned so that it is perpen-
dicular with the front edge of the 4. If necessary, make probe vertical adjust-
sample head (Figure 2). ments:
c. If adjustments are necessary, while a. Loosen the probe setscrew (Figure 1)
supporting the mandrel loosen the two and then raise or lower the probe
mandrel setscrews in the head, make within its mandrel, as necessary.
the adjustments, then carefully
b. When the bottom of the probe is
retighten the mandrel setscrews.
exactly level with the horizontal step,

Sample Probe Connector

Yoke

Stir Wire Slot in Mandrel

Mandrel Setscrews

Front of Sample Head

Figure 2. Elevated View of Osmometer Head

3LP700 Rev10
(4C35/4D35/3255/MK05 Service Manual)
Page 2 of 5

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 Mandrel

Make sure the stir wire

does not rub on the
sides of the slot in the
mandrel.

Raised V
Centerline This portion of the stir wire should
be parallel to sample probe, and
the space should be about .04
Alignment inches (1 mm).

Tool
Stir Wire
Sample Probe
Bottom of sample probe End of stir wire should
should rest on this level. rest on this small step.

Figure 3. Osmometer Alignment

gently retighten the probe setscrew 7. If necessary, make stir wire adjustments,
(overtightening will crack or distort as follows:
the thin plastic wall of the mandrel).
a. To realign the stir wire in the slot in
the mandrel, note the position of the
5. After completing any adjustments,
top of the yoke relative to the top of
recheck the probe alignment and level.
the head, then partially loosen the coil
core/yoke screw (see Figure 4) from
6. Check the stir wire alignment and level
the back of the head. Move the yoke/
(see Figure 3 notes). In the front-to-back
clapper/stir wire assembly side-to-side
direction, the stir wire should be even
until the stir wire is in the correct
with the sample probe.
position relative to the slot. Make
sure to maintain the previously noted

Figure 4. Coil Core Mounting Screw

3LP700 Rev10
(4C35/4D35/3255/MK05 Service Manual)
Page 3 of 5

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 yoke vertical position relative to the in the probe is in the back-to-front
head. Retighten the screw, making direction (parallel to the stir wire
sure that the yoke/clapper/stir wire action).
assembly does not rotate in place.
c. Gently retighten the probe setscrew
b. To adjust the vertical position of the (overtightening will crack or distort
stir wire, loosen the two stir wire the thin plastic wall of the mandrel).
setscrews (Figure 1) in the clapper,
then reposition the stir wire so that it 4. If necessary, make probe vertical adjust-
is resting on top of the small step, as ments:
shown in Figure 3.
a. Loosen the probe setscrew (Figure 1)
c. Firmly retighten the stir wire and then raise or lower the probe
setscrews. within its mandrel, as necessary.
b. When the bottom of the probe is
Cryoscope Instructions: exactly level with the horizontal step,
gently retighten the probe setscrew
1. Facing the instrument from the front, (overtightening will crack or distort
slide the alignment tool squarely up onto the thin plastic wall of the mandrel).
the mandrel, round-end first, with the
stir/freeze wire located as shown in 5. After completing any adjustments,
Figure 5. recheck the probe alignment and level.

2. Check the probe alignment by making 6. Check the stir wire alignment and level
sure that the probe shaft is lined up along (see Figure 5 notes). In the front-to-back
the raised V centerline of the alignment direction, the stir wire should be even
tool and the probe level by making sure with the sample probe.
that the end of the probe rests on the hori-
zontal surface below the raised V center- 7. If necessary, make stir wire adjustments:
line, as shown in Figure 5. Note that the
a. To realign the stir wire in the slot in
orientation of the alignment tool is 90º
the mandrel, note the position of the
from the way the tool is used with an
top of the yoke relative to the top of
osmometer.
the head, then partially loosen the coil
core/yoke screw (see Figure 4) from
3. If necessary, make probe centering adjust-
the back of the head. Move the
ments:
yoke/clapper/stir wire assembly side-
a. If aligning a metal probe, bend the to-side until the stir wire is in the cor-
metal probe stem gently by hand, as rect position relative to the slot.
necessary, to align the probe along the Make sure to maintain the previously
raised V centerline of the probe align- noted yoke vertical position relative
ment tool. to the head. Retighten the screw,
making sure that the yoke/clapper/stir
b. If aligning a non-metallic probe, DO
wire assembly does not rotate in
NOT ATTEMPT TO BEND THE
place.
PROBE; instead, loosen the probe
setscrew (see Figure 1) and then b. To adjust the vertical position of the
rotate the probe so that any curvature stir wire, loosen the two stir wire

3LP700 Rev10
(4C35/4D35/3255/MK05 Service Manual)
Page 4 of 5

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 Mandrel
Make sure the stir wire
does not rub on the
sides of the slot in the
mandrel.

Raised V
Stir wire should be parallel to
Centerline sample probe, and the space
should be about .06 inches
(1.5 mm).
Alignment
Tool
Stir Wire

Sample Probe
Bottom of sample
probe and end of
This level is not used with stir wire should rest
cryoscopes. on this level.

Figure 5. Cryoscope Alignment

setscrews (Figure 1) in the clapper,

then reposition the stir wire so that it
is resting on the same level as the end
of the sample probe (Figure 5).
c. Firmly retighten the stir/freeze wire
setscrew(s).

3LP700 Rev10
(4C35/4D35/3255/MK05 Service Manual)
Page 5 of 5

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Downloaded from www.Manualslib.com manuals search engine
 Sample Probe Replacement
4D3700
(3D3 Instruments with Serial Suffix A through B and
4D3 Instruments with Serial Suffix A through D)

Reference: Use this instruction with replace- leads as close to the terminal strip as pos-
ment part 4D3700. sible.

Tools Needed: Phillips screwdriver; flat- 5. Loosen the probe setscrew (C) and gently
bladed screwdriver; soldering pull the probe down and out of the man-
iron and SN62, 2% silver, drel, guiding the leads through the center
eutectic, rosin-core solder; hole so that they do not catch.
probe alignment tool and
instructions (included). B

CAUTION: Improper connections may

cause damage to the instrument.

Instruction:

1. Place an empty sample tube in the freez-

ing chamber to catch any extraneous
material that might fall in.

2. Turn off the power and unplug the instru-

ment.

3. Remove the two screws from the head

cover (A) and lift off the head cover.

6. Push the new probe (leads first) up

4. Identify the probe leads where they con- through the center hole of the mandrel. If
nect to the terminal strip (B). Snip off the friction alone does not temporarily hold

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4DP700 Rev2
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 the probe in position, slightly tighten the 4. At “[START] Ready?”, press START.
probe setscrew to provide just enough
friction to allow precise final adjustment. 5. At the end of the test, record the sample
probe resistance and the bin number, and
7. Use the probe alignment tool and instruc- then press STOP. The display will
tions to properly position the probe and change to “Probe Bin Test”.
stir/freeze wire. To set the correct sample bin number, fol-
low steps 6-8.
8. When the probe is in the position
required, tighten the probe setscrew just If the current sample bin number matches
enough to firmly hold the probe, but not the number just recorded, press STOP
crack or distort the thin plastic wall of the twice to exit the menu.
mandrel.
6. Press SETUP. The Supervisor/Operator
9. Use SN62, 2% silver, eutectic, rosin-core keyswitch must be in the Supervisor posi-
solder to make the new connections at the tion.
terminal strip. These connections are crit-
ical, so be sure to use good soldering Note: If you have a 4D3 cryoscope with a
techniques. suffix before “C”, the menu item “Set
Sample Bin #” is within the TEST
10. Arrange the probe leads so that they will menu, not the SETUP menu.
not touch the clapper or be pinched by the
head cover, and replace the cover. 7. At “Select Setup Item”, press < or > to
select “Select Sample Bin #”, and then
11. Plug in the power cord and turn on the press START.
instrument.
8. If the current sample bin number is not
12. Set the sample bin number (see the fol- the same as the one recorded in step 5,
lowing procedure). enter the correct number, press ENTER,
and then press STOP twice to exit the
13. Calibrate the instrument. (Recalibration menu.
is required each time the sample probe is
replaced.)

Set Sample Bin Number

1. If there is a Supervisor/Operator
keyswitch, turn it to the Supervisor posi-
tion (if required).

2. Press TEST. Press < or > to select

“Probe Bin Test”, and then press
START.

3. Place a sample of Probe Bin-Setting Fluid

into the freezing chamber.

4DP700 Rev2
(4D35 Service Manual)
Page 2 of 2

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 Sample Probe Replacement
3D3700

Reference: Use this instruction with replace- 2. Turn off the power and unplug the instru-
ment part 3D3700 (stainless ment.
steel) or 4D3102 (non-metallic),
and any of the following models 3. Remove the two screws from the head
of Advanced Instruments, Inc. or cover (A) and lift off the head cover.
Fiske Associates® osmometer or
cryoscope: 4. Unplug the sample probe connector from
Advanced Instruments Inc. the head transition board (B).
4250 Cryoscopes (Serial Suffix E - W)
4D3 Cryoscopes (Serial Suffix E - W) 5. Loosen the two mandrel setscrews (C).
3250 Osmometers (Serial Suffix C - T)
3D3 Osmometers (Serial Suffix C - T) B
Fiske Associates
Mark 2 Cryoscopes C
Mark 3 Osmometers

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver, probe
alignment tool and instruc-
tions (included).

CAUTION: Improper connections may cause

damage to the instrument.

Instruction:

1. Place an empty sample tube in the freez-

ing chamber to catch any extraneous
material that might fall in.
6. Lower the probe and mandrel together,
A down over the stir/freeze wire.

7. Push the connector and lead wires of the

new sample probe up through the hole in
the sample head, and raise the mandrel so
that the stir/freeze wire extends down
through the stir/freeze wire channel in the
mandrel.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 3DP700 Rev2
Service Center (U.S. 1-800-225-4034, outside North (3255/4D35/MK05 Service Manual)
America +US 1-781-320-9000). Page 1 of 2

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 8. Seat the mandrel firmly up into the man- 3. Place a sample of Probe Bin-Setting Fluid
drel hole, with the stir/freeze wire chan- into the freezing chamber.
nel perpendicular to the front edge of the
chassis and no gaps between the flange 4. At “[START] Ready?”, press START.
and the underside of the chassis.
5. At the end of the test, record the sample
9. Without overtightening (overtightening probe resistance and the bin number, and
can damage the mandrel), re-tighten the then press STOP. The display will
two mandrel setscrews to hold the man- change to “Probe Bin Test”.
drel in place.
To set the correct sample bin number, fol-
Note: The probe length is factory-adjusted to low steps 6-8.
the mandrel, and should not require
further adjustment. If the current sample bin number matches
the number just recorded, press STOP
10. Use the probe alignment tool and instruc- twice to exit the menu.
tions to properly position the probe and
stir/freeze wire. 6. Press SETUP. The Supervisor/Operator
keyswitch must be in the Supervisor posi-
11. Plug the sample probe connector into the tion.
head transition board.
7. At “Select Setup Item”, press < or > to
12. Arrange the probe leads so that they will select “Select Sample Bin #”, and then
not touch the clapper or be pinched by the press START.
head cover, and replace the cover.
8. If the current sample bin number is not
13. Plug in the power cord and turn on the the same as the one recorded in step 5,
instrument. enter the correct number, press ENTER,
and then press STOP twice to exit the
14. Set the sample bin number (see the fol- menu.
lowing procedure).

15. Calibrate the instrument. (Recalibration

is required each time the sample probe is
replaced.)

Set Sample Bin Number

1. If there is a Supervisor/Operator
keyswitch, turn it to the Supervisor posi-
tion (if required).

2. Press TEST. Press < or > to select

“Probe Bin Test”, and then press
START.

3DP700 Rev2
(3255/4D35/MK05 Service Manual)
Page 2 of 2

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 Sample Probe Replacement
4D3102

Reference: Use this instruction with replace- B

ment part 4D3106.

Tools Needed: Phillips screwdriver, flat- C

bladed screwdriver, probe
alignment tool and instruc-
tions (included).

CAUTION: Improper connections

may cause damage to the instrument.

Instruction:

1. Place an empty sample tube in the freez-

ing chamber to catch any extraneous
material that might fall in.

2. Turn off the power and unplug the instru-

ment. 6. Lower the probe and mandrel together,
down over the stir/freeze wire.
3. Remove the two screws from the head
cover (A) and lift off the head cover. 7. Push the connector and lead wires of the
new sample probe up through the center
4. Unplug the sample probe connector from hole of the mandrel, and raise the mandrel
the head transition board (B). so that the stir/freeze wire extends down
through the stir/freeze wire channel in the
5. Loosen the two mandrel setscrews (C). mandrel.

8. Seat the mandrel firmly up into the man-

A drel hole, with the stir/freeze wire chan-
nel perpendicular to the front edge of the
chassis and no gaps between the flange
and the underside of the chassis.

9. Without overtightening (overtightening

can damage the mandrel), re-tighten the
two mandrel setscrews to hold the man-
drel in place.

For additional information or technical assistance, 4D3P02 Rev1

please contact Advanced Instruments Hot-Line® replaced by 3DP700
Service Center (U.S. 1-800-225-4034, outside (4D35/3255 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

Downloaded from www.Manualslib.com manuals search engine

 Note: The probe length is factory-adjusted to To set the correct sample bin number, fol-
the mandrel, and should not require low steps 6-8.
further adjustment.
If the current sample bin number matches
10. Use the probe alignment tool and instruc- the number just recorded, press STOP
tions to properly position the probe and twice to exit the menu.
stir/freeze wire.
6. Press SETUP. The Supervisor/Operator
11. Plug the sample probe connector into the keyswitch must be in the Supervisor posi-
head transition board. tion.

12. Arrange the probe leads so that they will 7. At “Select Setup Item”, press < or > to
not touch the clapper or be pinched by the select “Select Sample Bin #”, and then
head cover, and replace the cover. press START.

13. Plug in the power cord and turn on the 8. If the current sample bin number is not
instrument. the same as the one recorded in step 5,
enter the correct number, press ENTER,
14. Set the sample bin number (see the fol- and then press STOP twice to exit the
lowing procedure). menu.

15. Calibrate the instrument. (Recalibration

is required each time the sample probe is
replaced.)

Set Sample Bin Number

1. If there is a Supervisor/Operator
keyswitch, turn it to the Supervisor posi-
tion (if required).

2. Press TEST. Press < or > to select

“Probe Bin Test”, and then press
START.

3. Place a sample of Probe Bin-Setting Fluid

into the freezing chamber.

4. At “[START] Ready?”, press START.

5. At the end of the test, record the sample

probe resistance and the bin number, and
then press STOP. The display will
change to “Probe Bin Test”.

4D3P02 Rev1
replaced by 3DP700
(4D35/3255 Service Manual)
Page 2 of 2

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 Cryoscope Sample Probe Versions
Frequently Asked Questions (FAQ)

A new, composite, stainless steel and plastic C) Why has the design been changed?
body cryoscope sample probe has been
Answer: The stainless steel probe, Type
released for use in the Advanced Instruments,
A, exceeded the thermal conductance lim-
Inc. single and multi-sample cryoscopes
its specified in ISO 5764, therefore, the
(Models 4D3, 4C3 and 4250).
plastic probe, Type B, was developed to
meet these new requirements. Type B,
A) What is the difference between the three
although compliant with the new ISO
versions of sample probe?
5764 standard, proved to be difficult to
Answer: Only the body material is differ- manufacture, which has led to the com-
ent. Type A used a brazed stainless steel posite design probe, Type C. This design
body; Type B used a one-piece plastic uses the strength of stainless steel up in
body; and Type C uses a composite stain- the mandrel, yet has the lower thermal
less steel and plastic body. All three use conductance of plastic where the probe is
the same high accuracy glass thermistor immersed in the sample.
as the sensing element.
D) My cryoscope has been operating without
B) Is this new probe used in the osmome- any problems. Do I need to change the
ters? probe? Will I see any performance
change?
Answer: No, this change is only for
cryoscopes and does not affect the all- Answer: If your cryoscope needs to be
stainless steel body sample probe used in compliant to the ISO 5764 standard and
the osmometers. you are currently using a Type A probe,

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4DP002 Rev1
Service Center (U.S. 1-800-225-4034, outside (4D3/4C3/3255 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 then you must change to the new Type C G) Does this change the part numbers used to
probe. If you are currently using a Type order replacement sample probes for my
B probe, then there is no reason to change cryoscope?
the probe at this time. There should be
Answer: If you are currently using the
no performance difference between the
one-piece plastic, Type B sample probe,
Type B and Type C sample probe designs.
then there is no change to the part num-
bers (listed below). The new, Type C
E) Is there any difference in the handling and
probes are completely interchangeable
maintenance of the three types of probe?
with the Type B probes.
Answer: During the initial installation of
the sample probe, the Type A stainless Sample Probe
steel probe may be bent slightly during Cryoscope Replacement Kit
the alignment procedure. Although the Model 4D3 4D3102
Type B and Type C probes are more flexi- Model 4C3 4C3701
ble than the Type A probe, they cannot be Model 4250 4D3102
permanently bent due to the plastic con-
struction. If a change is needed during
alignment, the probe should be rotated in
the mandrel to the desired position.
During cryoscope operation, the same
wiping and cleaning process may be used
for any of these probes. To minimize
potential damage, care should always be
taken to avoid hitting the glass thermistor
bead.

F) When were these probes used in

cryoscope production?
Answer: Cryoscopes shipped prior to
April 2002 contain the stainless steel
Type A probe.
Cryoscopes shipped from April 2002
through May 2003 contain the plastic
Type B probe.
Cryoscopes shipped in June 2003 and
later contain the new, composite stainless
steel/plastic Type C probe.

4DP002 Rev1
(4D3/4C3/3255 Service Manual)
Page 2 of 2

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 Stir/Freeze Coil Replacement
3D2404

Reference: Use this instruction with replace-

ment part 3D2404.

Tools Needed: Phillips screwdriver; flat- B

bladed screwdriver; Allen
wrench; soldering iron and
SN62, 2% silver, eutectic,
rosin-core solder; probe align-
ment tool and instructions
(included).

CAUTION: Improper connections may

cause damage to the instrument.
E
C
Instruction:

1. Place an empty sample tube in the freez-

ing chamber.

5. Identify the coil leads where they connect

2. Turn off the power and unplug the instru- to the terminal strip at TR1 and TR2 (C),
ment. and snip off the leads as close to the ter-
minal strip as possible.
3. Remove the two screws from the head
cover (A) and lift off the head cover. 6. Remove the Allen screw (D) that attaches
the yoke (E) and coil to the assembly.
4. Remove the two screws (B) that attach
the clapper to the yoke and lift the clapper 7. Remove the old coil and replace it with
to remove it. the new one, and then replace the Allen
screw.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 3DP404 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1 of 2

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 8. Feed the replacement coil leads through
the access hole and solder the lead ends to
TR1 and TR2 on the terminal strip.

9. Reassemble by reversing steps 2-4.

Note: Make sure the yoke level doesn’t inter-

fere with the head cover.

10. Use the probe alignment tool and instruc-

tions to properly position the stir/freeze
wire.

3DP404 Rev0
(4D35 Service Manual)
Page 2 of 2

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 Stir/Freeze Wire Replacement
3LH243/4LH243

Reference: Use this instruction with replace- 6. Use the probe alignment tool and instruc-
ment part 3LH243 or 4LH243. tions to properly position the stir/freeze
wire.
Tools Needed: Phillips screwdriver, flat-
bladed screwdriver, 5/64”
Allen wrench, probe align-
ment tool and instructions.

B
CAUTION: Improper connections may
cause damage to the instrument.

Instruction:

1. Turn off the power and unplug the instru-

ment. C

2. Remove the two screws from the head

cover (A) and lift off the head cover.
4250 / 4D3 Cryoscope
A

3. Loosen the two stir/freeze wire setscrews

(B) from the clapper.

4. Gently pull the stir/freeze wire (C) down C

and out through the mandrel.

5. Replace the old stir/freeze wire with the

new one and reassemble by reversing 3250 / 3D3 Osmometer
steps 2-4.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4LHP243 Rev4
Service Center (U.S. 1-800-225-4034, outside (4D35/3255 Service Manual)
North America +US 1-781-320-9000). Page 1 of 1

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Downloaded from www.Manualslib.com manuals search engine
 PCB520 Transistor Replacement Instruction
35010 to PCB520 Substitution
(Models 4D3, 3D3, 3250, 4C3, and 3900)

Reference: Use this instruction to install part 6. Remove the transistor socket Q1B from
PCB520, which is intended as a the rear panel and clear the mounting
direct replacement for part 35010 holes of any remaining hardware.
metal can FET transistor used on
7. Cut transistor socket Q1B from harness
the above models.
M20150.
Materials Included: 8. Strip wire insulation ¼” on four (4) wires
1 - PCB520 assembly formerly soldered to the transistor socket.
2 - H50606, #6x3/8 Sems Twist the stripped ends to prevent fraying.
2 - H50608, #6x1/2 Sems
2 - H63093, #6 large flat washers 9. Install the hex standoffs to PCB520 using
2 - 90049, 5/16 Hex swage standoffs #6x3/8 screws, with the flat end of the
2 - 91038, ¼” plastic hole plugs standoff against the board.
10. Connect white/red wire to terminal 1 (A).
Tools Needed: Wire cutters, wire strippers,
small flat-bladed screwdriver, 11. Connect white/brown wire to terminal 2
medium Phillips screwdriver. (B).
12. Connect brown wire to terminal 3 (C).
Warning-Hazardous Voltage
13. Connect black wire to terminal 4 (D).
Warning-Internal components may 14. Install completed assembly inside the unit,
be damaged by static electricity. against the rear wall with the terminal

4D3, 3D3 and 3250 Instruction:

1. Turn off the instrument power and discon-
nect the power cord.
2. Remove the intrument cover.
3. Locate transistor Q1B on left-rear of the
chassis. Q1B is the transistor nearest to
the top of the rear panel.
4. Remove the screws securing the protec-
tive black plastic cap on the outside rear
of the instrument.
5. Remove the old transistor and insulating
materials by gently prying with a flat-
bladed screwdriver.
A B C D

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® PCP520R Rev0
Service Center (U.S. 1-800-225-4034, outside North (4D35/4C35/3255/3905 Service Manual)
America +US 1-781-320-9000). Page 1 of 3

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 4C3 and 3900 Instruction:
1. Turn off the instrument power and discon-
nect the power cord.
2. Remove the intrument rear cover.
3. Locate transistor Q3 on the transistor heat
sink mounted to the bottom of the chassis.
Q3 is the transistor 2nd from the left.
4. Remove the screws securing the heat sink
to the chassis and gently lift out the heat
sink.
5. Remove the screws holding the old tran-
sistor and insulating materials.
E
6. Remove the old transistor and insulating
block (E) facing the left side. Use #6x1/2 materials by gently prying with a flat-
screws and large flat washers to secure bladed screwdriver.
swaged end of standoffs to the rear wall, 7. Remove the transistor socket Q3 from the
using the old transistor mounting holes. heat sink and clear the mounting holes of
15. Plug the old transistor lead holes using any remaining hardware.
the ¼” plastic hole plugs (F). 8. Cut transistor socket Q3 from harness
4C3160.
9. Strip wire insulation ¼” on four (4) wires
formerly soldered to the transistor socket.
Twist the stripped ends to prevent fraying.
10. Install the hex standoffs to PCB520 using
#6x3/8 screws, with the flat end of the
standoff against the board.
11. Connect red/white wire to terminal 1 (G).
12. Connect white/brown wire to terminal 2
(H).
13. Connect white/green wire to terminal 3 (I).
14. Connect black wire to terminal 4 (J).
F
15. Install completed assembly on the back
side of the heat sink where the previous
16. Replace the instrument cover and connect transistor socket was mounted. Use
the power cord. #6x1/2 screws and large flat washers to
17. Turn on the instrument and verify correct secure swaged end of standoffs to the heat
thermoelectric operation using the test sink, using the old transistor mounting
utility “A/D Test” (see user’s guide). holes. The ¼” plastic hole plugs are not

PCP520R Rev0
(4D35/4C35/3255/3905 Service Manual)
Page 2 of 3

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 J
I
H
G

used in this installation and may be dis-

carded.
16. Replace the instrument cover and connect
the power cord.
17. Turn on the instrument and verify correct
thermoelectric operation using the test
utility “A/D Test” (see user’s guide).

PCP520R Rev0
(4D35/4C35/3255/3905 Service Manual)
Page 3 of 3

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Downloaded from www.Manualslib.com manuals search engine
 Tube Ejector Spring Replacement
3D3312/4D3312
Reference: Use this instruction with replace- C B
ment part 3D3312 or 4D3312.

Tools Needed: Flat-bladed screwdriver, Tru-

arc pliers (for Osmometers).

CAUTION: Improper connections

may cause damage to the instrument.

Osmometer Instruction:

1. Turn off the power and unplug the instru-

ment.

2. Remove the four screws from the deck

(A), and remove the deck. Cryoscope Instruction:

3. Using a pair of Tru-arc pliers, remove the Remove the spring (D) from inside the
Tru-arc retainer ring (B) from inside the well and replace it with the new spring,
well. wide-end down.

4. Lift out the white Delrin elevator (C), and D

then the spring.

5. Replace the old spring with the new one

and reassemble by reversing steps 1-4.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4DP312 Rev1
Service Center (U.S. 1-800-225-4034, outside (4D35/3255 Service Manual)
North America +US 1-781-320-9000). Page 1 of 1

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Downloaded from www.Manualslib.com manuals search engine
 Yoke Replacement
3LH230

Reference: Use this instruction with replace-

ment part 3LH230.

Tools Needed: Phillips screwdriver, flat- B

bladed screwdriver, Allen
wrench, probe alignment tool
and instructions.

CAUTION: Improper connections may

cause damage to the instrument.

Instruction:

1. Turn off the power and unplug the instru-

ment.

2. Remove the two screws from the head

cover (A) and lift off the head cover.

C D

5. Replace the old yoke with the new one,

making sure the yoke is 90º and flush
against the back wall of the head.
3. Remove the two screws (B) that attach
the clapper to the yoke and lift the clapper 6. Reassemble by reversing steps 2-4.
to remove it.
Note: Make sure the yoke level doesn’t inter-
4. Remove the Allen screw (C) that attaches fere with the head cover.
the yoke to the assembly and remove the
coil (D). 7. Use the probe alignment tool and instruc-
tions to properly position the stir/freeze
wire.

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 3LHP230 Rev0
Service Center (U.S. 1-800-225-4034, outside (4D35 Service Manual)
North America +US 1-781-320-9000). Page 1

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Downloaded from www.Manualslib.com manuals search engine
 6. Printer
User Manual
Model 4D3550 Only
See manuals supplied with
other printer models

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Downloaded from www.Manualslib.com manuals search engine
 Model 4D3550 External Printer Specifications

Character Buffering: 1.5K standard Print Speed:

8K optional 38 lines per minute for 24 column
Approximately 9,500 byte 33 lines per minute for 32 and 40 column
capacity with option Paper:
installed. Table top: 2.25"W x 2.75"D; 0.44" I.D.
Panel mount: 2.25"W x 1.25"D
Print Method: Impact dot matrix
Power: 1.5 Watts (idle)
Character Matrix: 6 x 8 dot matrix 10 Watts (while printing)

Character Spacing: DC Voltage: 9-12 VDC

24 column: 12.8 characters/inch 100mA idle,
32 column: 17 characters/inch 1500mA with 100% printing
40 column: 21 characters/inch 2.9A peak with 100% printing

Line Feed Spacing: External Dimensions: 4"W x 4.5"L x 2"H

7.6 lines per inch...character mode
9.1 lines per inch...graphic mode Operating Temp: 5°C to 40°C
41°F to 104°F

For additional information or technical assistance,

please contact Advanced Instruments Hot-Line® 4MP540 Rev3
Service Center (U.S. 1-800-225-4034, outside (4D35SM)
North America +US 1-781-320-9000). Page 1 of 7

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 Installation 5. Slide the paper through the slot between
the paper compartment and the printer
Please follow the precautions listed below compartment. You can slide the paper in
when setting up your printer. They are about one-quarter inch before it stops.
designed to help you keep your printer work-
ing at its best.

• Plug your printer into an appropriate

grounded outlet.

• Place your printer on a flat hard surface,

like a tabletop.

• Keep your printer out of direct sunlight.

Connect the centronics cable to the 36-pin

centronics connector on the printer, and then
connect the DB25 cable to the female DB25
connector on the back panel of the instrument.
6. While holding the paper in place, press
Connect the DIN cable to the printer power the rocker switch to the paper feed posi-
jack on the back panel of the instrument, and tion. The printer will activate, and a rub-
then to the DC input on the printer. ber roller will pull the paper into the
printer compartment. Hold the switch in
the paper feed position until the paper
Installing the Paper emerges from the top of the printer mech-
anism.
1. Remove the printer cover by pressing on
the groove pattern to pop the front edge 7. When an inch of paper has emerged from
up. Lift off the cover. the top of the printer, release the paper
feed button and pull the paper through the
2. Press the rocker switch to the left. The printer until several inches are exposed.
light will go off.

3. Unroll several inches of the paper.

4. Cut a straight edge on the paper roll if it

is jagged. This will facilitate inserting
the paper into the printer.

8. Slide the paper through the slot in the

printer cover.

4MP540 Rev3
(4D35SM)
Page 2 of 7

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 2. Push down on the embossed grooves on
one or both sides of the printer cover until
the printer cover tilts.

9. Push the back of the printer cover down

and into place.

3. Lift off the printer cover. Push down on

the right side of the ribbon cartridge
where it is marked “PUSH”, and remove
the cartridge.
10. Press the front of the printer cover down
to lock in place.

4. Install the new cartridge. Be sure the ink

cartridge is inserted firmly to prevent
weak or irregular printing. The cartridge
must be properly seated and aligned for
best printing.

Changing an Ink Ribbon

When printing becomes faint or difficult to

see, the ribbon may be dried out or in need of
replacement. Before replacing the cartridge,
try advancing the ribbon to a new section by
holding down the paper feed switch for sever- 5. Turn the cartridge clockwise (marked by
al seconds. an arrow) to stretch the ribbon.

If the print impression is still weak, purchase

a new ribbon cartridge (Product #330541) and
install it using the following instructions.

1. Turn the printer offline by pressing the

left side of the rocker switch. The light
should go out. 6. Replace the cover. Replace the paper.

4MP540 Rev3
(4D35SM)
Page 3 of 7

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 Printer Test and Setup Setup Menu

The printer can be tested and setup using the Follow these steps to access the Setup Menu:
steps described in this section. However,
please note that the printer has been setup to 1. Unplug the printer at the outlet or at the
work with your instrument, as shipped. No back of the printer.
setup changes are necessary for correct opera-
tion. 2. Press and hold the left side of the rocker
switch as you plug in the printer. The
Before attempting to change your printer set- printer will advance the paper.
tings, be sure to disconnect the data cable
from the instrument. 3. After the paper advance has stopped,
count 3-5 seconds and release the switch.
The following is printed:
Printer Test

Follow these steps to test the printer: *** SETUP MENU ***
CONFIGURE... [NEXT/OK]

1. Unplug the printer at the outlet or at the

back of the printer. If you wait less than three or more than
five seconds, Ready... is printed and you
2. Press and hold the right side of the rocker will have to repeat steps 1 and 2 to access
switch as you plug in the printer. The the Setup Menu.
printer will print out a list of the current
configuration settings, and then run a 4. Press NEXT (left side of rocker switch)
continuous print test. To stop the print repeatedly to display the Setup Menu
test, press either side of the rocker switch. options. The list of menu options will
repeat as you continue pressing NEXT.
Below is a sample of what is printed when Following is a list of available Setup
you do the print test. Menu options:

*** PRINTER TEST *** *** SETUP MENU ***

Serial Version B122XL CONFIGURE... [NEXT/OK]
COLS=32 CUSTOM... [NEXT/OK]
FONT=5X8 RESET SEQ# [NEXT/OK]
INVERT=NO CONFIGURE... [NEXT/OK]
MAG=NONE
BUFFER: 9.5K
These options for customizing your printer
INT RAM: OK
EXT RAM: OK are explained below.
ROM: OK
EEPROM: OK
!”#$%&’()*+,-./0123456789:;< = >?@
“#$%&’()*+,-./0123456789:;<=>?@A
Ready...

4MP540 Rev3
(4D35SM)
Page 4 of 7

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 CONFIGURE... Mag
Magnification refers to the size of printed
The first menu item reads
type from your printer. Choices are:
CONFIGURE... [NEXT/OK]
NONE
[NEXT/OK] is a visual reminder that pressing Double Wide
the left side of the rocker switch will go to the Double High
next menu item, and that pressing the right Double Wide/High
side of the rocker switch will select the cur-
rent option. After choosing a magnification, the printer
will print Ready... to show that the printer is
Press OK (right side) to access the out of the Configuration Menu and the Setup
Configuration Menu. Menu, and is ready to print.

*** SETUP MENU ***

CONFIGURE... [NEXT/OK]
*** CONFIGURATION MENU *** CUSTOM...
*** CONFIGURATION MENU ***
The second Setup Menu item reads
LOAD DEFAULTS [NEXT/OK]
COLS=32 [NEXT/OK] CUSTOM... [NEXT/OK]
INVERT=NO [NEXT/OK]
FONT=5X8 [NEXT/OK] Press OK (right side) to access the Custom
MAG=NONE [NEXT/OK] Menu.
Ready...

*** SETUP MENU ***

Configuration parameters are described
CONFIGURE... [NEXT/OK]
below. CUSTOM... [NEXT/OK]
****** CUSTOM MENU ******
PRINT CUSTOM SETUP [NEXT/OK]
Cols
Use this parameter to set the number of char-
acters (columns) per line. Choices are 24, 32, Press OK (right side) to print the current
or 40 (the larger numbers represent a smaller, Custom setup. A sample printout follows.
more condensed appearance).

Invert
Use this parameter to change the orientation
of text in your printouts. Choose YES if you
want inverted (upside down) text, or NO if
you want non-inverted (right-side up) text.

Font
Choose from a 5 x 5 or a 5 x 8 dot matrix
print pattern. The 5 x 5 dot matrix pattern
produces only uppercase (capital) letters.

4MP540 Rev3
(4D35SM)
Page 5 of 7

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 YES - Voltage will be in a low state until the
*** SETUP MENU *** unit is busy, then voltage level goes
CONFIGURE... [NEXT/OK] high.
CUSTOM... [NEXT/OK]
****** CUSTOM MENU ******
PRINT CUSTOM SETUP [NEXT/OK] Online/Offline
AUTO SEQ=YES [NEXT/OK]
ZERO=0 [NEXT/OK] YES - Enables the rocker switch to turn the
POUND SIGN=# [NEXT/OK]
printer offline.
_(Underscore) [NEXT/OK]
BUSY INVERT=NO [NEXT/OK] NO - Disables the Online/Offline option.
ONLINE/OFFLINE=YES [NEXT/OK]
XON/XOFF 90/10 [NEXT/OK]
EXT CH SET=NO [NEXT/OK]
PRINT READY=YES [NEXT/OK] Ext Ch Set
Ready...
The option to use the extended character set is
available only when 8 data bits are chosen.
Customization parameters are described
below. YES - Allows you to use hexadecimal num-
bers above 80 (true only for 8 data
bits).
Auto Seq#
NO - Disables the Extended Character Set
NO - Don’t autoprint sequence number after option.
CR (carriage return).
YES - Autoprint sequence number after CR. Print Ready
YES - Prints Ready... upon power up.
Zero
NO - Disables printing Ready...
Choose how you want the zero character to
look in your printouts -- with or without a WARNING:
slash through the character. If you choose NO, hold down the left
side of the rocker switch for 4-6 sec-
onds to access the Setup Menu. Begin
Pound Sign timing when you plug in the unit and
the red light comes on. The paper
Choose to show pound as # symbol, or as the
feed motor does not run upon power-
British Sterling £ pound symbol.
up when Ready... is disabled.

_Underscore
Choose which symbol the same ASCII code
will print -- an _underscore, or a left arrow. RESET SEQ#...

The third Setup Menu item reads

Busy Invert RESET SEQ#... NEXT/OK]
NO - Voltage will be in a high state until the
unit is busy, then voltage level goes This menu option allows you to reset the
low. sequence number, which is the number of
print transactions since the last reset.

4MP540 Rev3
(4D35SM)
Page 6 of 7

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 To reset the sequence number, press OK (right
side). The sequence number will be reset to
zero and the printer will print Ready..., show-
ing it is no longer in the Setup Menu and that
the printer is ready to print.

To skip resetting the sequence number to

zero, press NEXT (left side). CONFIGURE...
is printed. Unplug and replug the printer to
return to printing mode. Ready... is printed.

4MP540 Rev3
(4D35SM)
Page 7 of 7

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Downloaded from www.Manualslib.com manuals search engine
 7. Schematics

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Downloaded from www.Manualslib.com manuals search engine
 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY
OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND PCB600 Assembly Drawing
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY

PCB600 Main Board Schematic (CPU) Rev8, Page 1 of 3

OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY

PCB600 Main Board Schematic (I/O) Rev8, Page 2 of 3

OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY

PCB600 Main Board Schematic (A/D) Rev8, Page 3 of 3

OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY

Interconnection Schematic Rev16 - Hitachi Processor

OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY

PCB605 Assembly Drawing

OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY

PCB605 Application Board Schematic Rev1

OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY
OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND 4D3605 Assembly Drawing
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY

PCB620 Assembly Drawing

OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY

PCB620 Processor Board Schematic Rev3

OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 THIS DESIGN, DRAWING, AND
PRINTS ARE THE PROPERTY

Interconnection Schematic Rev17 - Intel Processor

OF ADVANCED INSTRUMENTS.
REPRODUCTION OF ANY KIND
IS PROHIBITED EXCEPT WITH
SPECIFIC PERMISSION OF
ADVANCED INSTRUMENTS.

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 Appendix A
Symbol Definitions

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Downloaded from www.Manualslib.com manuals search engine
 Symbol Definitions
The following symbols may appear in product literature, or on intruments or product packaging.

On-Off Caution Hot Surface

Feed Dangerous Voltage

Interrupt Lifting Hazard

Test Calibrator

Start Content

Stop Control

Record Review Negative Control

Setup Positive Control

Calibration Flammable

Cancel; Delete Fragile

Functional Arrow Irritant

Printer Keep Dry

Enter Date Manufactured

RS232 Sterile

Bar Code Non-Sterile

Attention Serial Number

The Advanced® 3D3/4D3 Service Manual

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 Solution Use By; Expiration Date

Do Not Open Top Authorized Representative

Handle With Care Part Number

Toxic x Sufficient for [x] Tests

Use Blade To Open Open Here

Do Not Re-Use Low Fluid Level

For In Vitro Diagnostic Use Keep Hands Clear

European Conformity Latex-Free

Temperature Limit Diluent

See Instructions for

See Instructions
Temperature Guidelines

Lot Number Potential Puncture Hazard

Manufacturer Electronic Equipment -

Dispose of Properly

The Advanced® 3D3/4D3 Service Manual

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 Appendix B
Product Disposal and
Recycling

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Downloaded from www.Manualslib.com manuals search engine
 Product Disposal and Recycling

International concern about environmental and restrictions in effect to ensure

pollution resulting from improper disposal of proper disposal, and available locations
products and materials at the end of their use- where products can be sent.
ful life has resulted in an increase in legisla-
tion to control the methods and procedures • Contact Advanced Instruments Hot-
used to handle waste electrical and electronic Line Service:
equipment. While the regulatory status in
• 800 225-4034 (toll-free within the
some regions of the world has progressed to
USA and Canada; after normal busi-
the point where formal legislation is already
ness hours, dial extension 2191)
in effect, many other regions are in the
• +US 781-320-9000 (elsewhere)
process of creating similar legislation or
• 781-320-0811 (fax)
adopting that already in existence in other
areas. The result in the years ahead will be Service personnel will provide you
more stringent control over disposal of prod- with contact information for local dis-
ucts and recycling of their components once posal, or instructions for returning the
they are withdrawn from use. product directly to Advanced
Instruments.
Since regulations governing the disposal of
your instrument and accessories may vary
depending upon your geographic location, the
following guidelines are provided to assist
you in identifying the options available to you
once the decision has been made to replace or
dispose of this product:

• Contact the supplier who sold you the

product. Whether this was Advanced
Instruments itself, or one of its author-
ized dealers, this supplier should be
knowledgeable about the national and
local regulations governing disposal
and recycling of products in your area.
In some cases, this supplier may be
legally obligated to accept the product
from you and arrange for proper dis-
posal or recycling with no further
involvement on your part. Alternately,
the supplier can provide you with spe-
cific instructions for actions that you
can take to dispose of the product prop-
erly.

• Contact your local government agency

responsible for waste collection and
disposal. They can identify procedures

The Advanced® 3D3/4D3 Service Manual

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 Notes:

The Advanced® 3D3/4D3 Service Manual

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 Appendix C
Service Log

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Downloaded from www.Manualslib.com manuals search engine
 Service Log

To call for Service: (781) 320-5656 or (800) 225-1002

FAX (781) 320-0811


Model Number 3D3 or 4D3 Serial Number

Symptoms Repairs, Replacements

or Problems or Changes Serviced by

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 Symptoms Repairs, Replacements
or Problems or Changes Serviced by

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 Index

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Downloaded from www.Manualslib.com manuals search engine
 Index
A M
A/D Tests . . . . . . . . . . . . . . . . . . . . . . . . . .50 Maintaining the Instrument . . . . . . . . . . . .37
Application Board Description . . . . . . . . . .23
O
B Operator/Supervisor Keyswitch . . . . . . . . .17
Barcode Test . . . . . . . . . . . . . . . . . . . . . . . .53
Beeper Test . . . . . . . . . . . . . . . . . . . . . . . . .53
Block Bin Setting . . . . . . . . . . . . . . . . . . . .52 P
Power On Diagnostics . . . . . . . . . . . . . . . .50
C Power Supply . . . . . . . . . . . . . . . . . . . .21, 27
Probe Alignment . . . . . . . . . . . . . . . . . . . . .41
Control Board Set Description . . . . . . . . . .21
Processor Board Description . . . . . . . . . . .22
Conventions . . . . . . . . . . . . . . . . . . . . .11, 14

D R
Replacement Parts . . . . . . . . . . . . . . . . . . .32
Design Changes . . . . . . . . . . . . . . . . . . . . .30
Display/Printer Test . . . . . . . . . . . . . . . . . .53
S
F Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Sample Bin Setting . . . . . . . . . . . . . . . . . . .51
Freeze Amplitude . . . . . . . . . . . . . . . . . . . .46
Serial Number . . . . . . . . . . . . . . . . . . . . . .54
Service Assistance . . . . . . . . . . . . . . . . . . . .9
G Software Update . . . . . . . . . . . . . . . . . . . . .55
General Description and Purpose . . . . . . . . .9 Specifications . . . . . . . . . . . . . . . . . . . . . . . .7
General Overview . . . . . . . . . . . . . . . . . . .18 Stir Amplitude . . . . . . . . . . . . . . . . . . . . . .52
Stir Driver Noise . . . . . . . . . . . . . . . . . . . .47
I Suffix History . . . . . . . . . . . . . . . . . . . . . . .30
Instrument Software Update . . . . . . . . . . . .55 Supplies & Accessories . . . . . . . . . . . . . . .32

K T
Keypad Test . . . . . . . . . . . . . . . . . . . . . . . .53 Troubleshooting Table . . . . . . . . . . . . . . . .29
Key/Beeper Test . . . . . . . . . . . . . . . . . . . . .27

The Advanced® 3D3/4D3 Service Manual

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