OSCE Checklist ABG Sampling
OSCE Checklist ABG Sampling
Introduction
1 Introduce yourself to the patient including your name and role
3 If the patient is currently receiving oxygen therapy, note the oxygen delivery device and flow
rate
4 Briefly explain what the procedure will involve using patient-friendly language
9 Position the patient so that they are sitting comfortably, ideally with their wrist supported by
a pillow
10 Check if the patient has any pain before proceeding with ABG sampling
12 Gather equipment
14 Apply pressure over the radial and ulnar artery to occlude both vessels
15 Ask the patient to open their hand, which should now appear blanched
16 Remove the pressure from the ulnar artery whilst maintaining pressure over the radial artery
Preparation
18 Remove all equipment from its packaging so that it is easily accessible during the procedure
19 Attach the needle, with its protective cover intact, to the pre-heparinised ABG syringe
20 Position the patient's hand preferably on a pillow for comfort with the wrist extended by
approximately 20-30°
Procedure
21 Palpate the radial artery over the wrist of the patient's non-dominant hand to identify an ideal
puncture site
22 Once you have identified your planned puncture site, clean it with an alcohol wipe for 30
seconds and allow to dry before proceeding
23 Wash your hands again
25 Administer local anaesthetic and leave for a minimum of 60 seconds before performing
arterial sampling
26 Remove the protective cover from the ABG needle and then flush through the heparin from
the syringe
27 Hold the patient's wrist extended by approximately 20-30°
28 Palpate the radial artery with your non-dominant hand's index finger around 1cm proximal to
the planned puncture site
29 Warn the patient you are going to insert the needle
30 Holding the ABG syringe like a dart, insert the needle through the skin at the insertion site at
an angle of 30-45°
31 Continue to advance the needle slowly until you feel a sudden reduction in resistance and
see a rush of blood back into the ABG syringe
32 Allow the ABG syringe to self-fill
33 Once the required amount of blood has been collected remove the needle and apply
immediate firm pressure over the puncture site with some gauze or cotton wool
34 Engage the needle safety device
35 Remove the ABG needle from the syringe and discard immediately into a sharps bin
36 Place a cap onto the ABG syringe and carefully expel any air from the sample if present
38 Either the patient or a colleague should continue to apply firm pressure to the puncture site
for 3-5 minutes to reduce the risk of haematoma formation
41 Dispose of your gloves and equipment into an appropriate clinical waste bin
43 Take the ABG sample to be analysed as soon as possible after being taken