HF Series

Dual Piston Pump

Operator's Manual
902688 REV R

Teledyne SSI 349 N. Science Park Road State College, PA 16803

https://ssihplc.com Phone: 814-234-7311 Fax: 814-238-7532
Email: [email protected]

Use and Disclosure of Data Information contained herein is classified as EAR99 under the U.S. Export Administration Regulations.
Export, reexport or diversion contrary to U.S. law is prohibited.

i
 Warning Symbols and Task Specific Hazard Warnings:

The following warning symbols are present to alert you to risks that can arise when you install,
operate or maintain the HF-Class pump. Such risks include chemical exposure, electric shocks, and
others.

When the following symbols appear in the manual, as well as words such as “CAUTION, NOTE, or
WARNING,” their accompanying text identifies the specific risks and explains how to avoid them.
Teledyne SSI assumes no liability for the misuse of the information described in this manual in
regards to installation, repair, or operation of the HF-Class pump and its components.

ii
 TABLE OF CONTENTS

1 INTRODUCTION ..................................................................................................................................................... 1-1

Description of the HF Series Pump ................................................................................................................ 1-1

2 QUICK STARTUP GUIDE ...................................................................................................................................... 2-5

Specifications for the HF Series Pump .......................................................................................................... 2-6

3 INSTALLATION ...................................................................................................................................................... 3-7

Unpacking and Inspection .............................................................................................................................. 3-7
Location/Environment ..................................................................................................................................... 3-7
Electrical Connections .................................................................................................................................... 3-7
Solvent Preparation ......................................................................................................................................... 3-7

Instrument Installation ..................................................................................................................................... 3-9

Preparation for Storage or Shipping ............................................................................................................ 3-10

4 OPERATION ........................................................................................................................................................... 4-1

Front Panel Controls and Indicators .............................................................................................................. 4-1

Rear Panel Remote Input ................................................................................................................................. 4-1

Operator Instructions, Constant Flow............................................................................................................ 4-2
RS232 Information............................................................................................................................................ 4-7
REMOTE I/O ...................................................................................................................................................... 4-9

5 MAINTENANCE ...................................................................................................................................................... 5-1

Recommended Spare Parts Lists ................................................................................................................... 5-1
Filter Replacement ........................................................................................................................................... 5-1

Pump Head Assemblies .................................................................................................................................. 5-2

iii
 Piston Seals ...................................................................................................................................................... 5-4

Pistons .............................................................................................................................................................. 5-6

Check Valve Cleaning and Replacement ....................................................................................................... 5-6

Cleaning the Pump ........................................................................................................................................... 5-8

Lubrication ........................................................................................................................................................ 5-9

Fuse Replacement............................................................................................................................................ 5-9

6 QUICK GUIDE TO PROBLEM SOLVING .............................................................................................................. 6-1

7 WARRANTY STATEMENT ....................................................................................................................................... 2

8 COMPANY CONTACT INFORMATION .................................................................................................................... 4

iv
1 INTRODUCTION
This operator's manual contains information needed to install, operate, and perform
user maintenance on the HF Series Pump.

Description of the HF Series Pump

The HF Series High Performance Liquid Chromatography (HPLC) Pump is designed to
be a reliable component within a basic analytical or sophisticated research instrument
system. While ideal for HPLC applications, the HF Series Pump is also useful as a meter-
ing pump for general laboratory or industrial use.

Pump Features
• Automatically disables the motor if the pressure exceeds the programmable
maximum/minimum pressure limit.
• Integrated prime/purge valve.
• Inlet and outlet check valve.
• Graphical LCD front panel displays flow rate and operating pressure.
• Tactile response, chemically resistant front panel keypad.
• Servo motor design.
• RS232 serial communications for complete control and status monitoring.
• Rear panel input/output - remote start/stop and system error status.

Wetted Materials
Pump heads, check valve bodies, and tubing are made of type 316 stainless steel.
Other integrated materials are Zirconia (piston), UHMWPE (seals), PTFE (outlet filter
gaskets), PEEK, and Alumina (check valve internals).

Self-Flushing Pump Heads

Self-flushing pump heads provide continuous washing of the piston surface without
the inconvenience of a manual flush or gravity feed arrangement. The self-flushing
pump head uses a secondary seal and set of check valves to create a continuous and
positive flow in the area behind the high-pressure pump seal. The flushing solution
washes away any buffer salts that have precipitated onto the piston. If not removed,
these precipitates can abrade the high-pressure seal and cause premature seal failure,
leakage, and can possibly damage the pump.

1-1
 Self-Flushing Pump Head

Self-Flush and Seal Life

It is recommended that the Self Flush feature be used to improve seal life in a
number of applications. In particular, (as stated above) if pumping Buffers, Acids/Bases
or any inorganic solution near saturation, the pump should utilize the Self Flush feature.
With every piston stroke, an extremely thin film of solution is pulled back past the seal.
If this zone is dry (without use of Self Flush), then crystals will form with continuous
operation, which will ultimately damage the seal.

Another application where Self Flush is highly recommended is when pumping

Tetrahydrofuran (a.k.a. THR, Diethylene Oxide) or other volatile solvents such as
acetone (Note: THF and most solvents are compatible only with all-Stainless Steel
systems. THF will attack PEEK). Volatile solvents will dry rapidly behind the seal
(without the use of Self Flush), which will dry and degrade the seal.

100% IPA, 100% Methanol, 20% IPA/water mix or 20% Methanol/water mix are the
recommended options for the flush solution. Consult the factory if these are
unacceptable for the application.

PUMP MODIFICATION WHEN SELF-FLUSH IS NOT USED

If the self-flush feature is not used, it is strongly recommended to carefully
remove the self-flush seal with the seal tool provided and replace with the
provided guide bushing (reference the following instructions). If this is not
done; low flow rates, excessive noise and shortened pump life will result.

1-2
No Flush Conversion Procedure
Note: The conditioning of the seals may be required if the pump sits to long
after the conversion. Conditioning is covered in 3.0.

1. Using a hex tool, remove the two head

fasteners.

2. Carefully pull the pump head forward and off

the guide pins. Keep the self-flush in place.
Pull straight and slowly to prevent damage to
the piston. Note: the back-up washer may
remain on the piston. Carefully remove it and
install when reassembling.

3. Carefully pull the flush housing forward “O”-ring

and off the guide pins. Pull straight and Should remain
in the groove
slowly to prevent damage to the piston.
Note: assure that the “O”-ring remains in
the flush housing.

4. Using the supplied seal tool, carefully

remove from the seal in the direction shown. Seal

Inlet and Outlet check valve capsules can be

removed at this time also. Pliers may be
necessary.

1-3
5. Press the supplied plug into the wash Hole
housing as shown.
Carefully slide the guide bushing onto the
piston, in front of the backup plate.
Then, carefully slide the wash housing into
place. Push onto guide pins straight and
slowly to prevent damage to the piston. Guide
Bushing

6. Carefully slide the back-up washer onto Backup

the piston, in front of the wash housing. washer

Then, carefully slide the pump head into

place. Push onto guide pins straight
and slowly to prevent damage to the
piston.

7. sing a hex tool, reinstall fasteners. As

you tighten, alternate side-to-side until
snug. Turn 1 flat past snug.

1-4
2 QUICK STARTUP GUIDE
CAUTION: Always release pressure from the pump slowly. A rapid pressure release could
cause the pulse dampener diaphragm to rupture. Please refer to “Priming the Pump and
Flush Line” for more information.

Self-Flush
Interconnect Self-Flush
Outlet Tubing

Solvent Self-Flush
Soln.

Pump Inlet Self-Flush Prime/Purge

Pump Outlet
Tubing Inlet Tubing Valve

Self-Flush
Outlet Tubing

Prime/Purge
Valve

Self-Flush Pump
• Connect self-flush solution inlet tubing to the • Connect pump inlet tubing to bottom
bottom-left self-flush check valve, and the outlet of both pump heads as shown.
tubing to the top-right self-flush check valve as Make sure ferrule is in the correct position.
shown. • Attach syringe to Prime / Purge valve.
Note: The self-flush housings are interconnected at the • Open Prime / Purge valve by turning knob
factory for flow-through with a single inlet/outlet. counterclockwise one to two turns.
• Attach syringe to outlet self-flush tubing, using the • Draw syringe back to prime. Draw
1” lg piece of ¼” tubing. approximately 20 mL of fluid.
• Draw syringe back to prime. • Press PRIME button (P), continue to draw on
• After liquid has been pulled through the tubing into syringe until no bubbles are seen.
the syringe, remove syringe and place tubing in • Close Prime / Purge valve.
self-flush solution. • Press PRIME button (P).
*Replace self-flush solution weekly. • Remove syringe.
Note: If the self-flush feature is not used, refer to page * Replace solvent weekly.
3 for proper pump modification.

2-5
 Specifications for the HF Series Pump
Flow Rates ............................0 to 300 mL/min (reference chart below)
Pressure ...............................0 to 10,000 psi (reference chart below)
Pressure Accuracy ................± 0.5% of full-scale pressure

Pressure Zero Offset .............±1% of full-scale pressure (±100 psi)

Flow Accuracy .......................± 5%
Flow Precision ......................0.5% RSD
Dimensions (H x W x D) ........8.9" x 13.9" x 22.1" (22.6 x 35.3 x 56.1 cm)
Weight ..................................50 lb
Power ...................................120/240 VAC (Fluctuations not to exceed ±10%)
Environmental........................Indoor use only
Altitude ..................................2000 M
Temperature ..........................10 to 30° C
Humidity ................................20 to 90% Relative humidity
Remote Inputs .......................RS-232
Pulsation ................................±9.0% @ 150mL/min and 5000 psi (Constant Flow only)

10,000

9,000

8,000
Maximum Pressure (psi)

7,000

6,000

5,000
220 VAC
4,000
110 VAC
3,000

2,000

1,000

0
0 50 100 150 200 250 300
Flow Set Point (mL/min)

2-6
3 INSTALLATION
Unpacking and Inspection
Prior to opening the shipping container, inspect it for damage or evidence of
mishandling. If it has been damaged or mishandled, notify the carrier before opening the
container. Once the container is opened, inspect the contents for damage. Any damage
should be reported to the carrier immediately. Save the shipping container. Check the
contents against the packing list.

Location/Environment
The preferred environment for the HF Series Pump is normal laboratory conditions.
The area should be clean and have a stable temperature and humidity. The instrument
should be located on a stable flat surface with surrounding space for ventilation and the
necessary electrical and fluid connections. (Reference IEC 1010 installation category II,
and Pollution degree 2 environment.)

Electrical Connections
Unpack the HF Series Pump; position the pump so there is at least a four inch
clearance on all sides to permit proper ventilation. Then plug the pump into a properly
grounded electrical outlet.

WARNING: Do not bypass the safety ground connection as a serious shock hazard
could result.

Solvent Preparation
Proper solvent preparation will prevent a great number of pumping problems. The
most common problem is bubble formation, which may affect the flow rate consistency.
Aside from leaky fittings, the problem of bubble formation arises from two sources:
solvent out-gassing and cavitation. Filtration of HPLC solvents is also required.

Solvent Out-gassing and Sparging

Solvent out-gassing occurs because the mobile phase contains dissolved
atmospheric gases, primarily N2 and O2. These dissolved gases may lead to bubble
formation and should be removed by degassing the mobile phase before or during use.
The best practical technique for degassing is to sparge the solvent with standard
laboratory grade (99.9+%) helium. Helium is only sparingly soluble in HPLC solvents,
so other gases dissolved in the solvent diffuse into the helium bubbles and are swept
from the system. Solvent filtration is not an effective alternative to helium degassing.

It is recommended that you sparge the solvent vigorously for 10 to 15 minutes

before using it. Then maintain a trickle sparge during use to keep atmospheric gases
from dissolving back into the mobile phase. The sparged solvent must be continually
blanketed with helium at 2 to 3 psi. Non-blanketed sparged solvents will have
atmospheric gases dissolved back into the mobile phase within four hours.

3-7
 Solvent mixtures using water and organic solvents (like methanol or acetonitrile)
hold less dissolved gas than pure solvents. Sparging to reduce the amount of dissolved
gas is therefore particularly important when utilizing solvent mixture.

Even with sparging some out-gassing may occur. A backpressure regulator

installed after the detector flow cell will help prevent bubbles from forming and thus limit
baseline noise.

WARNING: Always release pressure from the pump slowly. A rapid pressure
release could cause the pulse damper diaphragm to rupture.

Cavitation
Cavitation occurs when inlet conditions restrict the flow of solvent and vapor bubbles
are formed during the inlet stroke. The key to preventing cavitation is to reduce inlet
restrictions. The most common causes of inlet restrictions are crimped inlet lines. Inlet
lines with tubing longer than 48" (120 cm) or with tubing of less than 0.085" (2 mm) ID
may also cause cavitation.

Placing the solvent reservoirs below the pump level also promotes cavitation. The
optimal location of the reservoirs is slightly above the pump level, but it is adequate to
have them on the same level as the pump.

Filtration
Solvent filtration is good practice for the reliability of the HF Series Pump and other
components in a HPLC system. Solvents should always be filtered with a 20 micron
filter prior to use (inlet filter not included with pump). This ensures that no particles will
interfere with the reliable operation of the piston seals and check valves. Solvents in
which buffers or other salts readily precipitate out will need to be filtered more often.
After filtration, the solvents should be stored in a closed, particulate-free bottle.

Solvents With Harmful Effects

All portions of the HF model pump that contact mobile phase are manufactured of
type 316 stainless steel, sapphire, ruby, Zirconia, PEEK, or fluorocarbon polymer.
Some of these materials are extremely sensitive to acids (including some Lewis acids)
and acid halides. Avoid using solvents that contain any amount of hydrochloric acid.
Some solvents you should specifically avoid are:

3-8
 Aqua Regia Hydrochloric Acid
Bromine Hydrofluoric Acid
Chlorine Anhydrous Hydrofluorsilicic Acid
Copper Chloride Hydrogen Peroxide
Ferric Chloride Iodine
Ferrous Chloride Mercuric Chloride
Freon 12 (wet) Guanidine
Hydrobromic Acid

In addition, some users of HPLC systems have observed that chloroform and carbon
tetrachloride slowly decompose to liberate hydrochloric acid, which, as noted above,
attacks stainless steel. Do not leave these solvents in the systems for a prolonged
period.

It is also recommended to avoid ammonium hydroxide. Although ammonium

hydroxide will not harm the pump itself, it is likely to damage the stator and rotor in
injection valves.

Instrument Installation

Mobile Phase Reservoirs

The mobile phase reservoir should be placed at the same level or slightly higher
than the pump, never below the pump, and the inlet tubing should be as short as
practical. These steps minimize pressure losses on the inlet side of the pump during
refill and help to avoid bubble formation. These steps are particularly important when
using high vapor pressure solvents (hexane, methylene chloride, etc.). Mobile phases
should be degassed, filtered and covered. (See Section 2.4.)

Self-Flush Solution
Self-flush heads require 250-500 mL of flushing solution. See section 1.1.4 for self-
flush solution recommendations. A pH indicator that will indicate the concentration of
salts in the solution is recommended as a reminder to change the solution. This flush
solution should be replaced with a fresh solution weekly to avoid frequent pump
maintenance.

WARNING: If you do not use the self-flush feature of this pump, you must
carefully remove the self-flush seal with the seal tool provided, replace with the
guide bushing provided (See illustration below). If this is not done; low flow
rates, excessive noise and shortened pump life will result.

Inlet Tubing
All inlet lines are supplied in a 48" (122 cm) length, with a 3/16” ID and a 1/4” OD,
and are made of FEP.

3-9
 Outlet Tubing
Outlet tubing (not supplied with the pump) should have a 1/8" outer diameter. It is
available in type 316 stainless steel. The tubing must be cut squarely with no burrs.
The tube itself should not be crimped and the center hole must be open. A tubing cutter
is recommended for cutting stainless steel tubing.

Priming the Pump and the Flushing Lines

Be sure all of the connections downstream of the prime/purge valve are closed.
Connect a syringe to the prime/purge valve. Open the prime/purge valve 1 to 2 turns
(counter-clockwise). Run the pump at a flow rate of 30 to 50 mL/min. Prime the pump
by pulling mobile phase and any air bubbles through the system and into the syringe (a
minimum of 20 mL). Close the prime/purge valve and stop the pump.

Preparation for Storage or Shipping

Isopropanol Flush
Disconnect the outlet tubing from the pump. Make sure the inlet tubing is connected
to the pump and solvent is available to the inlet. Open the prime/purge valve and use a
syringe to draw a minimum of 50 mL. Close the prime/purge valve and pump a
minimum of 50 mL of isopropanol to exit. Leave the inlet tubing connected to the pump.
Plug the outlet port with the shipping plug, leave a length of outlet tubing on the pump,
or cover the outlet port with plastic film.

Packaging for Shipping

CAUTION: Re-package in the original carton, if possible. If the original carton is not
available, wrap the pump in several layers of bubble wrap and cushion the bottom, top,
and all four sides with 2" of packaging foam. Although heavy, an HPLC pump is a
delicate instrument and must be carefully packaged to withstand the shocks and
vibration of shipment.

3-10
4 OPERATION
Front Panel Controls and Indicators

Prime/Purge Valve
The prime/purge valve vents the flow to atmosphere and permits efficient priming of
the HF Series Pump. When the valve is closed firmly (fully clock-wise), high-pressure
flow is directed to the Filter/Outlet port. When the valve is opened (counter clock-wise)
one-half to one full turn, pressure is vented and flow exits through the drain port in the
prime/purge valve stem assembly. Suction with a Luer tip syringe at the drain port will
purge air bubbles from the pump and reservoir lines (provided there are no open valves
to lines downstream at the injector/column interface). To prime the pump, draw about
20 to 30 mL of mobile phase.

Filter/Outlet
A high-pressure in-line filter is included at the output of the HF-Class pump. The
Filter/Outlet port is the high pressure filter closure, is designed for 1/8" o.d. tubing
connections.

Rear Panel Remote Input

An RS-232C modular jack is provided on the back panel. A computer, with appropriate
software, can be used as a remote control device for pump operation via this connection.

Figure 3-2. HF Series Pump Rear Panel

4-1
 Operator Instructions, Constant Flow
The HF Series Pump can be purchased as a Constant Flow (CF) pump.The major
components common to both versions include a servo motor, servo motor drive, and
Human Machine Interface / Programmable Logic Controller (HMI/PLC). This section
covers the operating instructions for the Constant Flow pump.

Figure 3-3. Splash Screen, Constant Flow

On power turn on, a temporary Splash Screen (Figure 3-3) will display for 10 seconds,
followed by the Operating Screen (Figure 3-4), which is the primary display screen. The
Splash Screen displays the SSI pump identification number, the firmware (program) part
number and revision, and the voltage level of the motor drive power bus, which is equal to
the AC input voltage.
The bus voltage level is dependent on the incoming AC line voltage, and will be
displayed either as “Low” (110VAC) or as “High” (220VAC). A high voltage bus will allow
the pump to operate at its maximum conditions. A low bus voltage will reduce the
maximum operating capability of the pump. See Section 1.2 for a full description of
operating parameters.
The CF main menu structure consists of three screens: the Operating Screen, the
Setup Screen, and the Password Screen. The left and right arrow buttons will cycle
through these screens in the following order:
Operating Screen ◄► Setup Screen ◄► Password Screen ◄► Operating Screen

4-2
 Figure 3-4. Operating Screen, Constant Flow

The Operating Screen contains the following controls and information:

• Fault Status display
• Run/Stop Status display
• Run/Stop pump control button
• Input Flowrate button
• Sign/Decimal input button
• Flow Rate display / input field, in ml/min
• Operating pressure display, in psi
• Menu navigation buttons

When the Operating Screen is initially displayed, the FLOW RATE input field will be
active, indicated by a blinking cursor located in the FLOW RATE field. To input a flow
rate in ml/min, press the corresponding number keypad buttons, located directly below
the display screen, and the DECIMAL BUTTON if required. It is not necessary to input a
zero decimal value – the decimal value will default to zero if no decimal value is entered.
Accepted flow rate values range from 0.0 to 300.0 ml/min. Press the ENTER button to
accept the new flow rate setting. Note that the flow rate set point will not be saved until
the ENTER button is pressed. The cursor will stop blinking, indicating that the field is no
longer ready to accept a new value. To input a new flow rate value, press the INPUT
FLOWRATE (ESC) button. As before, a blinking cursor indicates that the FLOW RATE
field is ready to accept a new set point.

4-3
 The RUN /STOP BUTTON will either start or stop the pump’s motor, depending on the
current condition. If the pump is stopped (default condition), the display will show “Run
Pump”; pressing the RUN/STOP BUTTON will run the pump. If the pump is running, the
display will show “Stop Pump”; pressing the RUN/STOP BUTTON will stop the pump.
Note that the flow rate set point must be non-zero and all faults must be cleared in order
for the pump to run.
The PRESSURE READOUT field will display the current operating pressure, read by a
pressure sensor located inside the pump cabinet.
The FAULT STATUS field will display the current fault status. If no faults exist, the field
will be blank. If a fault exists, the field will display “FAULT”. An existing fault status will
prevent the pump’s motor from running. All faults must be cleared to operate the pump.
Fault details can be found on the Setup Screen, which can be accessed by pressing the
right arrow button from the Operating Screen. The Setup Screen is described below.

Figure 3-5. Setup Screen,Constant Flow

4-4
 The Setup Screen contains the following controls and information:
• Detailed fault information
• Run/Stop pump control button
• Input data button
• Dual-purpose RESET DRIVE / negative sign input button for Flow Compensation
• High and Low Pressure Limit display / input fields
• Flow Compensation display / input field
• Menu navigation buttons

The FAULT INFORMATION field provides details on any existing fault conditions. If no
faults are present, the field will be blank. Possible fault messages include:
Low Pressure Fault (PLC)
• High Pressure Fault (PLC)
• Drive Fault (Unspecified, Motor Drive)
• Drive Fault Corrupt Flash (Motor Drive)
• Drive Fault A/D Out of Range (Motor Drive)
• Drive Fault Short Circuit (Motor Drive)
• Drive Fault AMP Overtemp (Motor Drive)
• Drive Fault Motor Overtemp (Motor Drive)
• Drive Fault Over Voltage (Motor Drive)
• Drive Fault Under Voltage (Motor Drive)
• Drive Fault Feedback Error (Motor Drive)
• Drive Fault Motor Phasing Error (Motor Drive)
• Drive Fault Following Error (Motor Drive)
• Drive Fault Over Current (Motor Drive)

The motor drive unit will attempt to automatically reset following a fault. The RESET
DRIVE button will also perform a reset of the motor drive unit, and also set the flow rate to
zero. If a motor drive fault cannot be resolved by the RESET DRIVE button, power cycle
the pump to clear the fault. If a motor drive fault condition persists, please consult the SSI
Service Department.
Multiple faults/warnings are displayed by using the “ESC” key to step turn the active
messages.
As with the Operating Screen, when the Setup Screen initially loads, the data entry
mode will automatically be active, indicated by a blinking cursor in the Low Pressure Limit
data input field. As before, the INPUT DATA BUTTON (ESC) will also activate data entry
mode to allow new values to be entered. To input a new value in the Low Pressure Limit
data field, use the corresponding numeric keypad buttons located directly below the
display to input a value between 0 and 9999. The Low Pressure Limit will disable the
motor and generate a Low Pressure Fault condition when the operating pressure drops
below this value. The value of the Low Pressure Limit should not exceed the value of the
High Pressure Limit. Press the ENTER button to accept the value; this will move the
cursor to the High Pressure Limit data field. To input a value in the High Pressure Limit
data field, use the corresponding numeric keypad buttons to input a value between 0 and

4-5
 9999. The High Pressure Limit will disable the motor and generate a High Pressure Fault
condition when the operating pressure exceeds this value. Press the ENTER button to
accept the value; this will move the cursor to the Flow Compensation data field.

The Flow Compensation data field allows you to adjust the flowrate of the pump to
accommodate various operating conditions, such as variability due to solvent
compressibility or temperature affects. This factor will adjust the flow rate in full-step
percentage increments. Note that the adjustment step size is calibrated using water as a
solvent, at room temperature. The actual percentage change seen in your system may
vary. Allowable range for this adjustment factor is -15% to +15%. To input a negative
value (decreased RPM), use the NEGATIVE BUTTON followed by a numeric value. To
input a positive adjustment (increased RPM), simply input the numeric value – no sign is
needed. Press the ENTER button the accept the value. This adjustment will become
active the next time the pump’s motor is started. If the motor is currently running, it is
necessary to stop and then start the pump for this value to be implemented. If the motor is
stopped, the adjustment will simply take effect the next time the motor is started.

Figure 3-6. Password Screen

The Password Screen restricts access to the Advanced Settings menu, which is used for
factory configuration of each instrument.

4-6
 RS232 Information

Figure 3-14. RS-232 Pinout

The HF Series Pump is configured for the following communication parameters:

9600 baud, no parity, 8 data bits and 1 stop bit.
Two cable assemblies with adapters are supplied: one “Quick Set Cable Assembly” to
allow user communications with the instrument, and one “PLC PROGRAMMING CABLE”
to allow access to the PLC, if necessary.
Refer to the following page for a complete list of available commands.

4-7
Command Description Display or PC Reply
RU Run Pump
ST Stop Pump
FOXXXX Flow Rate 4 Digits (Actual Flow Rate X 10) ** FO0500 = 50 ml/min
CC Read Actual Pressure & Flow Rate OK XXXX, XXX.X (or XX.XX)
PR Read Actual Pressure OK XXXX
CS Read Flow Rate XXXX,
Over Pressure Setting XXXX,
Under Pressure Setting XXXX, PSI
Head Type Number ** See Chart
Pump Running or Stopped ** 1 for Running, 0 for Stopped
Pressure Board Installed 1 for Installed, 0 for None
PI Read Flow Rate
Pump Running or Stopped ** 1 for Running, 0 for Stopped
Head Type Number ** See Chart
Pressure Board Installed 1 for Installed, 0 for None
External Voltage or Frequency Setup 1 for Voltage, 0 for Frequency
Frequency Controlled & Running 1 if Running under Frequency
Flow Control, else 0
Voltage Controlled & Running 1 if Running under Voltage Flow
Control, else 0
Upper Pressure Faulted 1 Faulted, else 0
Under Pressure Faulted 2 Faulted, else 0
Priming 1 if Priming, else 0
Keyboard Lockout 1, Keyboard Lockout, 0 Else
External Start 1 if Running by External Start, else
0
External Stop 1 if Stopped by External Stop, else
0
External Enable 1 if External Control Enabled, else
0
0 Unused
Motor Stall Fault 1 if Stall Faulted, 0 Else
UPXXXX Set the Upper Pressure Limit Must be in the pumps limits else
“ER/” is returned
LPXXXX Set the Lower Pressure Limit Must be in the pumps limits else
“ER/” is returned
SPXXXX Set Operational Pressure SP4500 = 4500 PSI Constant
Pressure Flow Output
RH Read Head Type Returns OK, 7/
ID Returns the Software and Version Number XXXXVXXX to PC
RE Resets Pump to Default Factory Settings Res
RC Returns the Compensation XX = Compensation factor with-
out rapid refill mode
RF Return the Fault Status bits X,X,X for Stall Fault, 1 for Faulted,
0 Else

4-8
 REMOTE I/O
The HF Series Pump is also equipped with a 9 pin DE-9 style connector, located on the
rear of the enclosure. This connections allows remote starting and stopping of the pump
through a dry contact relay, and also provides fault status of the pump via 2 dry contacts
(one normally open and one normally closed).

Pin Function
1 +24VDC
2, 3 Normal Closed contact indicating normal pump operation
4, 5 Normal Open contact indicating normal pump operation
6 Enable (Connect to pin 1 to activate Run/Stop inputs)
7 Run ( >100 ms high pulse to run pump)
8 Stop ( >100 ms high pulse to stop pump)
9 0 VDC (common)

4-9
5 MAINTENANCE
Cleaning and minor repairs of the HF model pump can be performed as outlined below.

Recommended Spare Parts Lists

Pump specific Recommended Spare Parts Lists are included with this pump in the
box. The spare parts list can also be accessed through our website, by entering the
pump’s serial number at the following address:
https://www.teledynessi.com/spare-parts-look-up
The pump serial number is included on the front cover of the manual, and on the
back of the pump.
Note: Lower than normal pressure, pressure variations, or leaks in the pumping system
can all indicate possible problems with the piston seal, piston, or check valves. Piston
seal replacement could be necessary after 1,000 hours of running time. See Section
5.2.3.

Filter Replacement

Outlet Filter

Figure 5-1, Outlet Filter

To service the outlet filter:

1. Using a 5/8" wrench to hold the filter housing at the pump, and a 1/2" wrench to
remove the filter closure, remove the filter closure assembly.
CAUTION: Do not use a metal object such as a screwdriver or paperclip to remove the
seal. Doing so can scratch the precision surface of the seat and may cause the filter
to leak.
2. Use a seal insertion/removal tool or a non-metallic object (such as a wooden
toothpick) to remove the large seal that remains in the housing.
3. Unscrew the old filter and remove the small seal from the filter closure.
4. Place one of the small seals included in the replacement element kit over one of the
new filters from the kit. Screw the new filter into the filter closure (finger tight).

5-1
5. Place one of the large seals from the replacement kit on the filter closure. Insert the
filter closure into the housing and tighten ¼ turn after seating.

Pump Head Assemblies

CAUTION: When working with aggressive or toxic solvents, residual amounts of

these chemicals could be present in the system.

Removing the Pump Head

The pump head assembly is shown below in Figure 4-1. Note that there is a guide
bushing used in place of the self-flush seal when the self-flush is not being used.
1. Turn OFF the power to the HF model pump.
2. Unplug the power cord.
3. Remove the inlet line from the mobile phase reservoir. Be careful not to crimp the
FEP tubing.
4. Remove the inlet line from the inlet check valve.
5. Remove the outlet line from the outlet check valve.
6. Use a 5/32 Allen wrench to carefully remove the four screws at the front of the
pump head, taking care not to lose the split-ring lockwashers.

CAUTION: Be careful not to break the piston when removing the pump head.
Twisting the pump head can cause the piston to break.

7. Carefully separate the pump head from the pump.

a. Move the pump head straight out from the pump and remove it from the piston.
Be careful not to break or damage the piston.
b. Remove the seal and seal backup washer from the piston if they did not stay in
the pump head.
c. Remove the O-ring.
8. Carefully separate the flush housing from the pump. Move the flush housing straight
out from the pump and remove it from the piston. Be careful not to break or damage
the piston. Also remove the self-flush seal from the piston if it did not stay in the
flush housing.

5-2
 Figure 4-1. Stainless Steel Self-Flushing Pump Head Assembly

Cleaning the Pump Head Assembly

If the piston seal or self-flush seal are going to be removed, it is recommended to
have a new set on hand to install after cleaning. It is not recommended to reinstall the
used piston seal or self-flush seal since they are likely to be scratched and damaged
during removal and would not provide a reliable seal if reused. If the seal is removed,
use only the flanged end of the plastic seal removal tool supplied with the seal
replacement kit. Avoid scratching the sealing surface in the pump head.

Inspect the piston seal cavity in the pump head. Remove any foreign material using
a cotton swab, or equivalent, and avoid scratching the sealing surfaces. Repeat for the
self-flush housing. Be sure no fibers from the cleaning swab remain in the components.

The pump head, check valves, and self-flush housing may be further cleaned using
a laboratory grade detergent solution in an ultrasonic bath for at least 30 minutes,
followed by rinsing for at least 10 minutes in distilled water. Be sure that all particles
loosened by the above procedures have been removed from the components before re-
assembly.

5-3
 Replacing the Pump Head
Refer to figure 4-1.
1. Carefully align the self-flush housing and gently slide it into place on the pump.
Make sure that the inlet valve is on the bottom and the outlet valve is on the top. If
misalignment with the piston occurs, gently push up on the piston holder.
2. Install the O-ring in its groove.
3. Line up the pump head and carefully slide it into place. Be sure that the inlet valve
is on the bottom and the outlet valve is on the top. Do not force the pump head into
place.
4. Ensuring the lockwashers are included, finger tighten the cap screws into place. To
tighten firmly, alternately turn screws 1/4 turn while gently wiggling the pump head
to center it.
5. Torque the cap screws to 35 in-lbs using a suitable torque wrench and 5/32 Allen
wrench adaptor.
6. Re-attach the inlet and outlet lines. Reconnect the self-flush lines. Change the
flushing solution.

Piston Seals
Lower than normal pressure, pressure variations, and leaks in the pumping system
can all indicate possible problems with the piston seal. Depending on the fluid or mobile
phase used, piston seal replacement is often necessary after 1000 hours of running
time.

Each replacement seal kit contains one seal, one backup washer, one self-flush
seal, one non-flush guide bushing, two seal insertion/removal tools, and a pad to clean
the piston when changing the seal.

Removing the Seals

1. Remove the pump head and self-flush assemblies as described in Section 4.2.1.
2. Remove the backup washer if it is present in the pump head.
3. Insert the flanged end of the seal insertion/removal tool into the seal cavity on the
pump head. Tilt it slightly so that flange is under the seal and pull out the seal.

CAUTION: Using any other “tool” will scratch the finish of the sealing surface and
create a leak.

4. Repeat the procedure for the low-pressure seal in the flush housing.
5. Inspect, and if necessary, clean the pump head as described in Section 4.2.2.

5-4
 Replacing the Seals
1. Place a high-pressure replacement seal on the rod-shaped end
of the seal insertion/removal tool so that the spring is visible when
the seal is fully seated on the tool. Insert the tool into the pump
head so that the open side of the seal enters first, facing the high-
pressure cavity of the pump head. Be careful to line up the seal
with the cavity while inserting. Then withdraw the tool, leaving the
seal in the pump head. When looking into the pump head cavity,
only the polymer portion of the seal should be visible.

Figure 4-2, 2. Place a self-flush replacement seal on the seal

Example of polymer side vs. insertion/removal tool so that the spring in the seal is visible when
energizer side of seal. Note the seal is on the tool. As in the previous step, insert the tool and
stainless steel energizer seal into the seal cavity on the flushing housing, taking care to line
shown. Seal could have up the seal with the cavity, and then withdraw the tool. When the
fluoropolymer o-ring energizer seal is fully inserted only the polymer part of the seal will be visible
instead (black o-ring). in the seal cavity.
NOTE: If the self-flush feature is not being used, install the
provided guide bushing in place of the self-flush seal.
3. Place seal back-up washer over the high-pressure seal in the pump head.
4. Attach the pump head as described in Section 4.2.3.
5. Condition the new seal as described below.

Conditioning New Seals

New seals should be conditioned prior to use. Conditioning is the process of running
the seals wet under controlled conditions to allow surfaces to seat and to prepare the
seal for operation.

Note: Use only organic solvents to condition new seals. Buffer solutions and salt
solutions should never be used to condition new seals. Recommended solvents are
HPLC-grade methanol and isopropanol, and water mixtures of either.

Suggested Conditioning Parameters: Using a restrictor coil or a suitable column,

run the pump with a 50:50 solution of isopropanol (or methanol) and water for 30
minutes at the back pressure and flow rate listed under PHASE 1 in the following chart
and according to the pump head type. Then run the pump for another 15 minutes under
conditions for PHASE 2 in the following chart, according to pump flow and pressure
capabilities.

Seal Conditioning Parameters

Flow Rate Setting Pressure (psi) Phase 1 Pressure (psi) Phase 2
20-30% of Maximum
*1,000 *1,500
Pump Flow
* - or 95% of Maximum pump capability

5-5
 Pistons

Cleaning the Piston

1. Once the pump head and self-flush housing are removed as described in Section
4.2.1, gently remove the seal back-up plate by using either a toothpick or small
screwdriver in the slot on top of the pump housing.
2. Grasp the metal base of the piston assembly so that you avoid exerting any side
load on the zirconia rod, and carefully pull the piston off of the carrier.
3. Use the scouring pad included in the seal replacement kit to clean the piston.
Gently squeeze the piston within a folded section of the pad and rub the pad along
the length of the piston. Rotate the piston frequently to assure the entire surface is
scrubbed. Do not exert pressure perpendicular to the length of the piston, as this
may cause the piston to break. After scouring, use a lint-free cloth, dampened with
alcohol, to wipe the piston clean.
4. Grasp the metal base of the piston assembly, and carefully reinstall it onto the
carrier.
5. Place the seal back-up plate back into the pump housing.

Replacing the Piston

1. Remove the pump head as described in Section 4.2.1.
2. Gently remove the seal back-up plate by using either a toothpick or small
screwdriver in the slot on top of the pump housing.
3. Grasp the metal base of the piston assembly so that you avoid exerting any side
load on the zirconia rod, and remove the piston from the slot in the carrier by sliding
it up.
4. Grasp the metal base of the replacement piston assembly, and carefully install it
onto the carrier.
5. Place the back-up plate back into the pump housing.
6. Attach the pump head as described in Section 4.2.3.

Check Valve Cleaning and Replacement

Many check valve problems are the result of small particles interfering with the
operation of the check valve. As a result, simply cleaning the pump head with the
appropriate laboratory apparatus may resolve any issues.

Check Valve Cleaning

1. To clean pump c heck valves, remove the pump head and immerse the entire head
into a laboratory ultrasonic cleaner.
2. Sonicate for about 30 minutes using a standard cleaning solution. Rinse the pump
head thoroughly with distilled water.

5-6
3. Replace the pump head assembly.
4. Run the pump at 1 mL/min (3 mL/min for a 40 mL pump head) with distilled water
for fifteen minutes. Always direct the output directly to a waste beaker during
cleaning (do not recycle).
5. If this procedure does not return the pump to proper performance, the check valves
should be replaced. An example of new check valves from their package can be
seen in Figure 4-3 below.

Check Valve Replacement

Figure 4-3, New Check Valves from package and proper orientation

1. Remove the pump head assembly.

2. Remove the check valve housings and capsules from the pump head, being careful
not to scratch the sealing surfaces in the pump head. If necessary, use a seal
removal tool to remove the capsules from the pump head.

CAUTION: Be careful not to break the piston when removing the pump head.
Twisting the pump head can cause the piston to break.

CAUTION: Make sure check valve is kept in the above position to avoid
losing parts

3. Hold one new check valve assembly as shown in Figure 4-3 and unscrew the
protective cap. With the check valve assembly maintained in the above position,
thread it into the proper pump head port until it is snug Install the other check valve
assembly similarly.
4. Reinstall the pump head assembly as described in Section 4.2.3.
5. Tighten the check valve housings to 75 inch-lbs, or enough to seal at maximum
pressure.
6. Reattach the solvent inlet and outlet lines.
7. Reconnect the self-flush lines to the self-flush check valves

5-7
 Self-Flush Check Valves
Self-flush check valves can be replaced without removing the pump head of self-
flush assembly, and do not require any tools. When installing new check valves, notice
the outlet has a transparent washer, and the Inlet has a cross ball retainer. Also, the
words INLET and OUTLET should be visible on the top of the self-flush check valves.

Figure 4-4, Check Valve Assembies for Pump Head and Self-Flush Housing

Cleaning the Pump

1. Prepare the following solvents, utilizing the solvent preparation methods detailed in
Section 2.4.
a. 100% isopropanol
b. 100% filtered, distilled water
c. 20% nitric acid/water solution.
2. Direct the pump outlet line to a waste beaker.
3. Set the flow rate to 150 mL.
4. Pump 100% isopropanol through the pump for 3 minutes.
5. Pump 100% filtered, distilled water through the pump for 3 minutes.

WARNING: Use standard laboratory procedures and extreme care when handling strong
acids and bases.

6. Pump a 20% nitric acid/water solution through the pump for 3 minutes.
7. Flush the pump with 100% filtered, distilled water for at least 3 minutes.

5-8
 8. Pump 100% isopropanol through the pump for 3 minutes.
The pump is now prepared for any mobile phase or short- or long-term shutdown.

Cleaning the cabinet

Cabinet may be cleaned with tap water or a mild soap solution.

Lubrication
The HF model pump has modest lubrication requirements. The bearings in the pump
housing and piston carrier are permanently lubricated and require no maintenance. A
small dab of a light grease such as Lubriplate 630-AA on the cam is the only
recommended lubrication. Be sure not to get lubricant on the body of the piston carrier, as
this can retard its movement and interfere with proper pumping.
Note: Keeping the interior of the pump free of dirt and dust will extend the pump's useful
life.

Fuse Replacement
The HF Series Pump is protected by two fuses,
located inside the power entry module at the rear of
the cabinet, in series with the AC input line.
Troubleshooting the fuses is straightforward. With
the power cord connected and the master power
switch ON, if the rear case fan does not run, check
the two fuses in the power entry module. To gain
access to these fuses, gently pry off the cover plate
with a small flat-bladed screwdriver. Replace with
fuses of the same rating.

Figure 4-5, Fuse Replacement

5-9
 6 QUICK GUIDE TO PROBLEM SOLVING
You Notice This May Mean Possible Cause You Should
1. Uneven pressure trace. 1. Bubble in 1. Solvent not properly degassed. 1. Check to be certain that mobile phase is properly
2. Pressure drops. check valve. 2. Fittings are not tight. degassed.
3. Pump shuts OFF. 2. Leaks in 3. Mobile phase not properly 2. Check connections for leaks by tightening fittings.
4. No flow out the outlet check system. filtered. 3. Prime the system directly from the outlet check
valve. 3. Dirty check 4. Particles from worn piston seal valve.
valve. caught in check valve. 4. Clean or replace the check valves. See Section
4. Bad check 5. Plugged inlet. 4.4.
valve. 5. Clean or replace inlet. See Section 4.1.1.

1. Uneven pressure trace. 1. Leaks in 1. Fittings not tight. 1. Check all connections for leaks.
2. Pressure drops. system. 2. Long usage time since last 2. Replace piston seal. See Sections 4.2 and 4.3.
3. Fluid between the pump 2. The piston seal change. 3. Check the piston for salt deposits. Clean as
head and the chassis. seal(s) are 3. Salt deposits on seal necessary. See Section 4.2.5.
worn. (especially if buffered aqueous
mobile phases are used
without the self-flush head.)
Pump makes a loud clanging or Piston carrier is 1. Cap nut screws on the pump 1. Check cap nut screws on pump head. Tighten if
slapping noise (intermittent catching in head are loose. necessary.
contact with cam). piston guide. 2. Seal(s) are worn. 2. Replace seals.
3. Piston guide is worn 3. Replace piston guide and seals. See Sections 4.2
4. Salt build-up on piston carrier and 4.3.
from use of buffers. 4. Consider changing to a self-flushing pump head if
5. Excess lubricant on piston using buffers.
carrier. 5. Clean excess lubricant and dirt off piston carrier.
See Section 4.7.

Pump runs for 50 pump Lower pressure 1. Mobile phase is not properly 1. Check to be certain the low-pressure limit is set to
strokes, and then shuts down. limit is activating. filtered. 0 psi.
2. Particles from worn seal 2. Only increase the low-pressure limit after the
trapped in the system (e.g., pump attains operating pressure.
tubing, filters, injection valve, 3. Contact service technician.
column inlet).
1. Pump shuts down after run Clog in fluid 1. Remove and clean the bulkhead outlet filter. See
is called even with no system. Section 4.1.
column connected. 2. If the problem persists, remove tubing from system
2. Pump runs to maximum one piece at a time until you find the clogged
pressure and shuts down. piece. Most clogs occur outside the pump itself.

No power when pump turned Blown fuses in 1. Power surge. 1. Replace only with the appropriate fuses 10A
ON. Fan does not run. the power entry 2. Internal short. 250Vac.
module. 2. Contact service technician if problem persists.
Front panel appears OK but 1. Power surge. 1. Replace only with the appropriate fuse.
pump motor does not run. 2. Internal Servo Control Board 2. Contact service technician if problem persists.
error.

6-1
7 WARRANTY STATEMENT
Teledyne SSI (SSI) warrants that instruments or equipment manufactured by the company for a period thirty-six
(36) months from date of shipment to the original purchaser (or to the drop ship location as indicated on the
Purchase Order from the original purchaser), against defects in materials and workmanship under normal
installation, use and maintenance. Products sold by SSI but not manufactured by SSI carry the Original
Manufacturer’s Warranty, beginning as of the date of shipment to SSI’s original purchaser. Expendable items and
physical damage caused by improper handling or damage caused by spillage or exposure to any corrosive
environment are excluded from this warranty. The warranty shall be void for Polyetheretherketone (PEEK)
components exposed to concentrated Nitric or Sulfuric acids which attack PEEK, or methylene chloride, DMSO or
THF which adversely affect UHMWPE seals and PEEK tubing. Any defects covered by this warranty shall be
corrected by replacing or repairing, at SSI’s option, parts determined by SSI to be defective.

Spare or replacement parts and accessories shall be warranted for a period of twelve (12) months from date of
shipment to the original purchaser against defects in materials and workmanship under normal installation, use
and maintenance. Defective Product will be accepted for return to SSI only if the request for return is made within
thirty (30) days from the time of discovery of the alleged defect, and prior to return, the original purchaser obtains
a Return Goods Authorization (RGA) number from SSI, and provides SSI with the serial number of each
instrument to be returned.

The warranty shall not apply to any Product that has been repaired or altered except by SSI or those specifically
authorized by SSI, to the extent that such repair or alteration caused the failure, or to Product that has been
subjected to misuse, negligence, accident, excessive wear, or other causes not arising out of a defect in material
or workmanship.

The warranty shall not apply to wear items, specifically:

• Check Valves
• Piston and Wash Seals
• Pistons
• Pulse-Damper Diaphragms
• Inlet Lines
• Filter Elements

The following is the exclusive procedure by which to make claims under this warranty. Customer shall obtain
SSI’s oral or written authorization to return the Product and receive a Return Goods Authorization (RGA) number.
The Product must be returned with the RGA number plainly visible on the outside of the shipping container to SSI.
It must be securely packed in a rigid container with ample cushioning material, preferably the original packaging.
All claimed defects must be specified in writing, including the RGA number, with the written claim accompanying
the Product. Freight costs for the return of reported defective Product from the original purchaser to SSI is the
responsibility of the original purchaser. Freight costs for the return of reported defective spare parts is the
responsibility of SSI. SSI shall specify the freight carrier for returns. SSI shall bear the expense of return shipment
to original purchaser (or to the drop ship location as indicated on the Purchase Order from the original purchaser).

If it appears to SSI that any Product has been subjected to misuse, negligence, accident or excessive wear, or is
beyond the warranty period, the original purchaser and/or customer shall be notified promptly. SSI shall
communicate its finding and provide an estimate to repair such Product at the then current rates for parts and
service. SSI shall either repair the Product per customer’s authorization or shall return such Product not repaired
to customer at customer’s expense. SSI may invoice customer for the freight costs of any Product shipped back
to the original purchaser and/or customer by SSI which is not covered under the warranty.

Limitations of Warranty. THE FOREGOING WARRANTIES AND LIMITATIONS ARE CUSTOMER’S EXCLUSIVE
REMEDIES AND ARE IN LIEU OF ALL OTHER WARRANTIES, EXPRESS OR IMPLIED, INCLUDING
WITHOUT LIMITATION ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR
PURPOSE.

2
3
8 COMPANY CONTACT INFORMATION

Teledyne SSI
349 Science Park Road
State College, PA 16803
800-441-HPLC (4752)
Technical Support: 814-234-7311
Sales: Option 2
Technical & Service Support: Option 3