Scribd
Upload
74 views

Microscope PDF

Microscopes are instruments that use one or more lenses to magnify small objects. There are two main types - light microscopes, which use visible light, and electron microscopes, which use electron beams. The document discusses the parts and workings of light microscopes, including how simple microscopes use one lens while compound microscopes use multiple lenses to provide higher magnification through their eyepiece and objective lenses.

Uploaded by

Axestetikriey Hales
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
74 views

Microscope PDF

Microscopes are instruments that use one or more lenses to magnify small objects. There are two main types - light microscopes, which use visible light, and electron microscopes, which use electron beams. The document discusses the parts and workings of light microscopes, including how simple microscopes use one lens while compound microscopes use multiple lenses to provide higher magnification through their eyepiece and objective lenses.

Uploaded by

Axestetikriey Hales
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 26

Introduction of Microscopes

Microscope: Instrument used to make


small object appear larger. Simple and
Compound.
Simple Microscope vs. Compound Microscope
One lens More than one lens used
Microscopes
There are two types of microscope
◦ Light
◦ Electron

Properties of microscopes
— Magnification: The process of enlarging something in
appearance.
— Resolution: Ability to distinguish the individual parts of a
object. This is another term for the sharpness
of an image.
Microscopes
Magnification and Lenses

— The lenses in light microscopes magnify an object


by bending the light that passes through them.

— On a hand lens you will see that the lens is curved,


not flat. The center of the lens is thicker than the
edge.
— A lens with this curved shape is called a convex
lens.
Microscopes
Magnification and Lenses
— When light passes through the sides of the lens it
bends inward.
— When this light hits the eye, the eye sees the object
as larger than it really is.
Microscopes
Compound Microscope Magnification

— Compound microscopes contain multiple lenses which makes


it more powerful than just one lens.

— The first lens, near the specimen, magnifies the object. Then a
second lens, near the eye, further magnifies the enlarged
image.
— The total magnification of the microscope is equal to the
magnifications of the two lenses multiplied together.

Light passes through a specimen and then


through two lenses, as seen in the picture on
this slide.
Microscopes
How it works:
— The first lens makes an object look 10 times bigger than it actually
is.

— The second lens makes the object look 40 times bigger than it
actually is.

— The total magnification of the microscope is 10 x 40, or 400.


PARTS OF THE COMPOUND LIGHT MICROSCOPE

ocular lens/eyepiece

body tube

revolving/rotating nosepiece
objective lenses
arm
stage clips

stage
coarse adjustment knob
condenser
fine adjustment knob
diaphragm

light source/ base


illuminator
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Ocular Lens / Eyepiece

magnify the image of the specimen.


• Contains a lens to _______

• The usual magnification is ___


10 X.

• Some microscopes have ___


two ocular lenses.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Body Tube

• It ________
connects the eyepiece to the objective lenses.

alignment of the microscope


• It ensures the correct ________
components to correctly direct
_____ the light from the
specimen into the viewer’s eye
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Arm

• It ________
connects the body tube to the base.

hand should be around the arm when _______


• One ____ carrying the
microscope (the other should be under the base
____)
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Base

• It ________
supports the weight of the microscope.

• It contains the _________


electronics and __________.
light source

• One hand should be _____


under the base while _______
carrying
the microscope (the other hand should be holding the
arm).
PARTS OF THE COMPOUND LIGHT MICROSCO
Light Source / Illuminator

mirror

upwards through the _____________


• It sends light _______ condenser lens

hole in the stage onto the ________


and through the ____ specimen

on the slide.
• mirrors to ______
Older microscopes used to use ______ reflect the
ambient light upwards.
PARTS OF THE COMPOUND LIGHT MICROSCO
Revolving/Rotating Nose Piece

• The _____________
objective lenses are attached to it.

• Rotating switch
_______ the nose piece allows you to _____
between the different lenses.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Objective Lenses
4X

10 X

40 X

magnify the image of the


• These lenses further _______
specimen.

• The magnifications are usually ___


4 X , ____
10 X and ____
40 X .

• There are usually __


3 lenses but some
have _4_ lenses.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Objective Lenses

• As the power increases, the magnification becomes larger


_____
smaller
, but the field of view (visible area) becomes _______.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Coarse Adjustment Knob

• The ____
first knob you should use, and always under ___
low
high power.
power. Never use it in ____
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Fine Adjustment Knob

• The ____
first knob you should use, and always under ___
low
high power.
power. Never use it in ____

• The ______
second knob you should use under ______
higher
exact focusing.
power for _____

• Both knobs move the _____


stage up and down
to help put the specimen in _____.
focus
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Fine Adjustment Knob

• Some microscopes have the two knobs located


one on top of the other
___________________.

• The smaller one on the bottom is always the ____


fine
adjustment knob.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Stage AND Stage Clips

• The stage is where you place the ____


slide which contains
the specimen
________.

• It contains a ____
hole that allows ____
light to pass through the
stage and onto the specimen.

• The stage clips ______


secure the slide on the stage.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Condenser Lens

focuses light
• The lens under the stage that ___________
hole in
from the illuminator through to the ____
the stage.
PARTS OF THE COMPOUND LIGHT MICROSCOPE
Diaphragm

focuses light
• The lens under the stage that ___________
hole in
from the illuminator through to the ____
the stage.

• It contains a dial that rotates to _____


adjust the
amount of light that reaches the specimen.
_____________
Microscope

https://www.youtube.com/watch?v=Swcz_TJMz0I

You might also like