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BF 00571142

The document summarizes research on the flavonoids found in several species of plants from the Dipsaceae family and Prunus spinosa. Two flavonoid compounds, querectimeritrin and luteolin 7-glucoseide, were isolated from nine Dipsaceae plants through recrystallization and chromatography. Kaempferol 7-rhamnoside was identified as the main flavonoid glycoside found in the leaves of Prunus spinosa through enzymatic hydrolysis, UV/IR spectroscopy, and comparison to a reference sample. The investigation of additional flavonoids in these plants is ongoing.

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34 views1 page

BF 00571142

The document summarizes research on the flavonoids found in several species of plants from the Dipsaceae family and Prunus spinosa. Two flavonoid compounds, querectimeritrin and luteolin 7-glucoseide, were isolated from nine Dipsaceae plants through recrystallization and chromatography. Kaempferol 7-rhamnoside was identified as the main flavonoid glycoside found in the leaves of Prunus spinosa through enzymatic hydrolysis, UV/IR spectroscopy, and comparison to a reference sample. The investigation of additional flavonoids in these plants is ongoing.

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Tymoteusz Dziedzic
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UDC 547.

QUERCIMERITRIN AND LUTEOLIN 7-GLUCOSIDE IN S O M E


S P E C I E S OF D I P S A C A C E A E

G. N. Zemtsova and V. A. Bandyukova

Khimiya Prirodnykh Soedinenii, Vol. 4, No. 4, p. 247, 1968

We have isolated the total flavonoids from nine plants--representatives of the family D i p s a e a c e a e - - c o l l e c t e d in the
region of Kislovodsk and the Teberda reserve and, by recrystallization and chromatography on Kapron we have obtained
individual substances.
On the basis of the results of elementary analysis, IR and UV spectroscopy, the determination of physical con-
stants, and the products of c l e a v a g e it has been shown that the herbs Cephalaria balkharica E. Busch., Cephalaria g i -
gantea (Ldb.) Bobr., Dipsacus strigosus W i l l d . , Scabiosa bipinnata C. Koch., Scabiosa caucasica M. B., and Scabiosa
ochroleuca L. contain luteolin 7 - ~ - D ' - g l u c o p y r a n o s i d e . Quercetin 7-~-D-glucopyranoside (quercimeritrin) has been
found in ttie herb Cephalaria balkharica E. Busch., in the flowers of Cephalaria gigantea (Lbd.) Bobr., and in the a b o v e -
mentioned species of Scabiosa.
The investigation of the other flavonoids contained in these plants is continuing.

6 M a y 1967 Pyatigorsk Pharmaceutical Institute

UDC 547

STUDY OF T H E FLAVONOIDS OF P R U N U S SPINOSA

V. A. Makarov

Khimiya Prirodnykh Soedinenii, Vol. 4, No. 4, p. 248, i968

The flavonoids were extracted from the leaves of Prunus spinosa L. [ 1 - 4 ] with 70% ethanol after preliminary e x -
traction with petroleum ether and chloroform. The ethanol was evaporated off and the aqueous residue was additionally
treated with chloroform in order to e l i m i n a t e the chlorophyll more completely; after this the flavonoids were extracted
s u c c e s s i v e l y with ethyl acetate and a mixture of ethyl acetate and methanol (80 : 20). After part of the solvents had
been distilled off, the flavonoids were precipitated with a 5-fold amount of chloroform. Paper chromatography of the
purified alcoholic extracts in various systems of solvents showed the presence of seven flavonoid glycosides.
The total flavonoids obtained were separated in a column of polyamide s o r b e n t - K a p r o n . On desorption with 2 5 -
35% ethanol, the first portions of the eluate contained glycoside 1. Desorption with 45% ethanol yielded glycoside 2.
In this communication, we give the results of a study of glycoside 2.
Glycoside 2 was recrystallized from aqueous ethanol. Mp 232-233 ° C, [ a ] ~ --176 ° (c 0.1; methanol). After h y -
drolysis with 1% HzSO4 for 2 hr in the water bath, kaempferolwas isolated (yield 67%). The paper chromatography of the hy-
drolysate after neutralization with barium carbonate showed that glycoside 2 contains L- rhamnose as the sugar component.
From the Rf values in various systems and a mixed melting point, the glycoside was shown to be i d e n t i c a l with
k a e m p f e r e l 7-rhamnoside kindly given to us by Prof. H6rhammer (Munich). The results obtained together with the results
of UV and IR spectroscopy and also those of e n z y m a t i c hydrolysis permit the monoside isolated from the leaves of Prunus
spinosa to be identified as kaempferol 7 - O - a - L - r h a m n o f u r a n o s i d e .

REFERENCES

1. L. HOrhammer, L. Endres, H. Wagner, and F. Richthammer, Arch. Pharmazie, 290, 342, 1957.
2. K. Fuehs, Dissertation, Mtinchen, 1959.
3. V. A. Makarov, collection" Subjects of a Combined Conference on Questions of Spa Treatment, Pharmacy,
and Pharmacology [in Russian], Pyatigorsk, p. 328, 1967.
4. V. A. Makarov, c o l l e c t i o n ; Subjects of the XVI-th Scientific Conference of Physiologists of the Seven Russian
Soviet Federated Socialist Republics with the participation of Biochemists and Pharmacologists [in Russian], Ordzhoni-
Ikidze
, L P. 225, 1967.

15 August 1967 Pyatigorsk Pharmaceutical Institute

211

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