Agilent Cary 8454

UV-Visible Spectroscopy
System

Installation Guide


Notices
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Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

In This Guide…
This handbook describes what you have to do to install your UV-Visible
spectroscopy system. Most of the handbook deals with installing a complete
system, that is, the spectrophotometer, computer and printer. However, in the
second half of this handbook you will find additional information about
installing accessories, accessory boards, configuration, and software
installation.

1 Fast Installation
Use this chapter as an overview of the different actions you must perform to
install your UV-Visible spectroscopy system. If you are an experienced user of
analytical equipment from Agilent Technologies, this may be all you need. If
you need more information, this chapter guides you to other relevant areas in
this or another handbooks.

2 Installation and Start Up

Use this chapter if you need more detailed information about the installation
process.

3 Good Measurement Practices

Use this chapter if you require information about general operating conditions
to perform accurate and reliable measurements, for example, handling
samples, solvents and cells.

4 Installing Accessories
This chapter describes how to install the simple mechanical and optical
accessories that are available to expand the capabilities of your UV-Visible
spectrophotometer.

5 Installing the UV-Visible Software

This chapter describes what your computer requires, and what you must do to
install the software.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 3

 6 LAN Communication, Installation, Connection and Configuration
This chapter describes how to set up LAN communication between your
Agilent ChemStation and your UV-Visible spectrophotometer.

A Safety Information
This chapter contains relevant safety information.

4 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

Contents
1 Fast Installation 9
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems 10
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer
Systems 11
Installing Software Only 13

2 Installation and Start Up 15

Site Requirements for Your Spectrophotometer 17
Power considerations 17
Power cords 18
Space 18
Environment 19
Unpacking Your Spectrophotometer 20
Installing your UV-Visible Spectrophotometer 21
Instrument overview 21
Setting up the Spectrophotometer 24
Installing Your Computer 26
Setting up the computer 26
Starting the Spectrophotometer 27
Turning On 27
Instrument Warm-up 29
Starting the Agilent ChemStation Software 30
Operational Checkout 30

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 5

Contents

3 Good Measurement Practices 31

General Considerations 32
Spectrophotometer Design 32
Making Measurements 32
Sample Cell or Cuvette Material 33
Optical Specifications of Cells 34
Apertured Cells or Cuvettes 35
Flow Cells 36
Handling and Maintaining Cells or Cuvettes 37
Solvents 39
Sample Preparation 40
Photosensitive Samples 41
Stirring and Temperature Control 41
Checklist for Best Results 42
Inserting a Cell 44

4 Installing Accessories 45
Thermostattable Cell Holder 46
Installing a Thermostattable Cell Holder 47
Cell Stirring Module 48
Installing the Cell Stirring Module 49
Tubing Connections and Operation 50
Long Path Cell Holder 52
Installing the Long Path Cell Holder 53
Installing a Sample Cell 53
Optical Filter Wheel 55
Installing the Filter Wheel Assembly 57
Removing the Filter Wheel Assembly 58

5 Installing the UV-Visible Software 59

Agilent ChemStation Computer Details 60

6 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Contents

Minimum PC Requirements for Agilent ChemStation 60

Installing the Agilent ChemStation Using the DVD 63
Overview 63
Uninstalling Pre-existing Agilent UV-Visible ChemStation Software 63
Installation Procedure 64
Configuring Your System 72

6 LAN Communication, Installation, Connection and Configuration 75

Installing and Configuring TCP/IP on the PC 76
Installation of the TCP/IP protocol 76
Installing the LAN Interface in your UV-Visible Spectrophotometer 79
Connecting the Spectrophotometer to the PC 80
Single Instrument Installation 80
Multiple Instrument Installation 80
Setting the IP Address of the Spectrophotometer 81
Using the Agilent BootP Service 82
What is the Agilent BootP Service? 82
Addresses 83
Using the Agilent BootP Service Program 84
Configure the Agilent BootP Service Program 85
Configure the Agilent BootP Service 87
Configuring the G1369C LAN Card Using BootP 89
Using the Agilent ChemStation Configuration Editor to Configure the
Spectrophotometer 91
What is the Agilent ChemStation Configuration Editor? 91
Configuring an Agilent Cary 8454 UV-Visible Spectrophotometer 92
Troubleshooting LAN Communication 94
Power failure reported on the Agilent ChemStation 94
Frequent buffer overrun in Agilent Cary 8454 logbook 95

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 7

Contents

A Safety Information 97
Protective Earth 97
Disconnecting Device 97
UV Light Source 98
Equipment Class 98
Installation category 98
Pollution level 98

Index 99

8 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide

1
Fast Installation
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems 10
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible
Spectrophotometer Systems 11
Installing Software Only 13

Installation is straightforward, but if you have difficulties, or need more

information, references to detailed installation information are given.
Use this chapter if you are:
• installing a preconfigured UV-Visible spectroscopy system from Agilent
(including spectrophotometer, computer and software),
• installing a UV-Visible spectroscopy system not purchased as a
preconfigured system, or
• installing software only.

Agilent Technologies 9
1 Fast Installation
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems

Installing Preconfigured Agilent Cary 8454 UV-Visible Systems

If you have purchased one of these systems, the software is pre-installed and
preconfigured. You need only install and switch on the hardware.
✔ Ensure you have space and an appropriate power supply.
For more information, see “Site Requirements for Your
Spectrophotometer” on page 17.
✔ Unpack the system—check for damage and contents against packing list.
For more information, see “Unpacking Your Spectrophotometer” on
page 20.
✔ Install your UV-Visible spectrophotometer.
• Connect line power to the rear.
• Connect the waste tubing underneath the sample pan.
For more information, see “Installing your UV-Visible
Spectrophotometer” on page 21.
✔ Install your computer and printer.
• Connect the keyboard and mouse.
• Connect the monitor to line power and to the computer.
• Connect the printer to line power and to the computer.
• Connect the computer to the spectrophotometer with the LAN cable.
For more information, see “Installing Your Computer” on page 26 and
Chapter 6, “LAN Communication, Installation, Connection and
Configuration”.
✔ Switch on the spectrophotometer (make sure the sample area is clear), wait
2 minutes for the lamps to ignite. When you are ready to operate your
spectrophotometer, switch on the computer and printer.
For more information, see “Starting the Spectrophotometer” on page 27.

10 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Fast Installation 1
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer Systems

Installing Non-Preconfigured Agilent Cary 8454 UV-Visible

Spectrophotometer Systems

You must install and configure the software.

✔ Ensure you have space and an appropriate power supply.
For more information, see “Site Requirements for Your
Spectrophotometer” on page 17.
✔ Unpack the system - check for damage and contents against packing list.
For more information, see “Unpacking Your Spectrophotometer” on
page 20.
✔ Install your UV-Visible spectrophotometer.
• Connect the line power to the rear.
• Connect the waste tubing underneath the sample pan.
For more information, see “Installing your UV-Visible
Spectrophotometer” on page 21.
✔ If your computer and printer are not supplied by Agilent, ensure that the
requirements for proper operation of the software are met.
For more information, see “Minimum PC Requirements for
Agilent ChemStation” on page 60.
✔ Connect the computer to the spectrophotometer with the LAN cable.
For more information, see “Installing Your Computer” on page 26 and “LAN
Communication, Installation, Connection and Configuration” on page 75.
✔ Install your computer and printer.
• Connect the keyboard and mouse.
• Connect the monitor to the power supply and to the computer.
• Connect the printer to the power supply and to the computer.
• Connect the computer to the spectrophotometer with the LAN cable.
For more information, see “Installing Your Computer” on page 26.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 11

1 Fast Installation
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer Systems

✔ Switch on the spectrophotometer (make sure sample area is clear) and wait
two minutes for the lamps to ignite. When you are ready to operate your
spectrophotometer, switch on the computer and printer.
For more information, see “Starting the Spectrophotometer” on page 27.
✔ Install the Agilent ChemStation software.
For more information, see “Installing the Agilent ChemStation Using the
DVD” on page 63.
✔ From the Windows Start menu, start the Agilent ChemStation software.
For more information, see “Starting the Agilent ChemStation Software” on
page 30.
✔ Ensure that your software and spectrophotometer operate properly by
taking a blank measurement or performing the automatic self-test in the
diagnostic section of your software.
For more information, see “Operational Checkout” on page 30.
✔ If you need information about solvents, sample preparation, blank
measurements, cuvette handling or how to insert a cuvette, see Chapter 3,
“Good Measurement Practices”.
✔ Install any accessories.
For simple accessories see Chapter 4, “Installing Accessories”; for complex
accessories, see the instructions supplied with the accessory or the
Installation and Maintenance section of the Agilent ChemStation online
help.

12 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Fast Installation 1
Installing Software Only

Installing Software Only

✔ Unpack the software—check for damage and check the contents against the
packing list.
✔ Ensure that your computer and printer meet the requirements for proper
operation of the software.
For more information, see “Minimum PC Requirements for
Agilent ChemStation” on page 60.
✔ Switch on the spectrophotometer (make sure the sample area is clear) and
wait two minutes for the lamps to ignite. When you are ready to operate
your spectrophotometer switch on the computer and printer.
✔ From the Windows menu install the Agilent ChemStation software.
For more information, see “Installing the Agilent ChemStation Using the
DVD” on page 63.
✔ From the Windows Start menu start the Agilent ChemStation software.
For more information, see “Starting the Agilent ChemStation Software” on
page 30.
✔ Ensure that your software and spectrophotometer operate properly, by
taking a blank measurement or by performing the automatic self-test in the
diagnostic section of your software.
For more information, see “Operational Checkout” on page 30.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 13

1 Fast Installation
Installing Software Only

14 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide

2
Installation and Start Up
Site Requirements for Your Spectrophotometer 17
Unpacking Your Spectrophotometer 20
Installing your UV-Visible Spectrophotometer 21
Installing Your Computer 26
Starting the Spectrophotometer 27
Starting the Agilent ChemStation Software 30
Operational Checkout 30

The following topics are covered in this section:

• “Site Requirements for Your Spectrophotometer” on page 17 contains
information about electrical requirements such as power lines, power
cords, etc. It also gives recommendation for minimum available bench space
and environmental conditions in the laboratory.
• “Unpacking Your Spectrophotometer” on page 20 contains information
about what to do when the packing material is damaged or the shipment is
incomplete. It includes a list of the items which are supplied with the
instrument and computer.
• “Installing your UV-Visible Spectrophotometer” on page 21 gives an
overview of the instrument and explains buttons, indicators and the line
power switch. It explains where and how to connect line power,
communications and waste drain tubing.
• “Installing Your Computer” on page 26 describes how to install the
computer and peripherals, e.g. monitor, mouse, keyboard and printer.
• “Starting the Spectrophotometer” on page 27 describes the turn-on
procedure for the spectrophotometer and the warm up time required to
fulfill all specifications.

Agilent Technologies 15
2 Installation and Start Up

• “Starting the Agilent ChemStation Software” on page 30 describes how you

start your Agilent ChemStation software.
• “Operational Checkout” on page 30—use this section to ensure that your
spectrophotometer, computer and the UV-Visible operating software is in
proper operating condition. There is an optional automated self-test which
can be used for a quick check of the spectrophotometer. This self-test can
only be executed after the instrument has warmed up for at least one hour.

16 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installation and Start Up 2
Site Requirements for Your Spectrophotometer

Site Requirements for Your Spectrophotometer

Power considerations
Your spectrophotometer can operate on a line voltage of 100–240 V AC with a
line frequency of 50 or 60 Hz. Maximum power consumption is 220 VA.
The instrument power supply accepts any line voltage supplied in the above
mentioned range. Consequently there is no voltage selector in the rear of the
spectrophotometer. There are no externally accessible fuses, because
automatic electronic fuses are implemented in the power supply.

To disconnect the instrument from line power, disconnect the power cord from the
WARNING
supply. The power supply still uses some power, even if the power switch on the
front panel is turned off.

The computer and InkJet or LaserJet printers accept a nominal line voltage of
110 or 220 V AC with a line frequency of 50 or 60 Hz. For more information,
see the documentation that comes with your computer and/or printer.

Connecting your instrument to a line voltage that is higher than specified causes a
WARNING
shock hazard, and damage may occur to your instrument.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 17

2 Installation and Start Up
Site Requirements for Your Spectrophotometer

Power cords
Different power cords are offered as options with the spectrophotometer. The
female end of each of the power cords is identical. This connects to the
power-input socket at the rear of the spectrophotometer. The male end of each
of the power cords is different and designed to match the wall socket of a
particular country or region.

Do not replace the mains power cord with one that is inadequately rated. When replacing
NOTE the mains power cord, use only a mains power cord with a rating equivalent to the one
supplied with the instrument. See the instrument installation guide for detailed mains
power cord rating information.

Always operate your instrument from a power outlet which has a ground
WARNING
connection. Always use the power cord designed for your region.

Do not position the equipment so that it is difficult to operate the

disconnecting device.

Space
The spectrophotometer is 34.4 cm (13.5 inches) wide, 56.0 cm (22.0 inches)
deep and 18.5 cm (7.3 inches) high (see Figure 1 on page 22). It weighs 14 kg
(30.86 lbs) and will fit on almost any desk or laboratory bench. It needs an
additional 2.5 cm (1.0 inches) of space on either side to allow for the
circulation of air for cooling, and approximately 8 cm (3.1 inches) in the rear
for electrical connections.
The computer, including the keyboard, is approximately 50 cm (19.7 inches)
wide and 60 cm (23.6 inches) deep.
Additional space is required for accessories like pumps and autosamplers.

18 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installation and Start Up 2
Site Requirements for Your Spectrophotometer

Environment
Table 1 Suitable environmental conditions for the 8454 UV-Vis

Condition Altitude Temperature Humidity (%RH)

non-condensing

Non-operating Up to 4,600 m - 40 and 70 °C 90%

(Storage or shipping) (14,950 ft.) (-4 to 158 °F)

Operating within Up to 2,000 m 5-40 °C <95%

specifications (6,500 ft.) (41-104 °F)

For optimum analytical performance, it is recommended that the ambient temperature of

NOTE the laboratory be between 20-25 °C and be held +/- 2 °C throughout the entire working day.

If you use the instrument at environmental temperatures higher than 50°C (122°F),
WARNING
the rear of the instrument may get hot.

Do not store, ship or use your instrument under conditions where temperature
CAUTION
fluctuations could cause condensation within the instrument. Condensation will
damage the electronics. If your instrument was shipped in cold weather, leave it in its
box and allow it to warm up slowly to room temperature to avoid condensation.

Avoid air circulation of varying temperatures (like from refrigerators or air

conditioners) and exposure to direct sun light to ensure the instrument
operates within performance specifications.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 19

2 Installation and Start Up
Unpacking Your Spectrophotometer

Unpacking Your Spectrophotometer

Upon receipt of your spectrophotometer, computer and printer, inspect the

shipping containers for any signs of damage. If the containers or cushioning
material are damaged, save them until the contents have been checked for
completeness and the spectrophotometer, computer or printer have been
mechanically and electrically checked. If the shipping container or cushioning
material is damaged, notify the carrier as well as Agilent Technologies. Save
the shipping material for the carrier’s inspection.
Check the contents of the shipping container against the list below. If any
items are missing or obviously damaged, call Agilent Technologies.
To avoid injury to personnel or damage to equipment, always use a two or more
WARNING
people or suitable lifting device when moving the instrument.

✔ Unpack the spectrophotometer. You should have the following components:

• Power cord to connect the spectrophotometer to line power.
• LAN Interface, if you connect the spectrophotometer through a LAN
• Waste tubing to connect the sample pan of your spectrophotometer to a
waste container.
• Checkout sample (caffeine solution).
✔ Unpack the computer and its accessories. You should have the following
components:
• Computer and power cable
• Keyboard
• Mouse
• Display unit, connecting cable and power cable
• Printer, connecting cable and power cable
• Printer accessories, for example, ink cartridge in case of a DeskJet
printer

20 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installation and Start Up 2
Installing your UV-Visible Spectrophotometer

Installing your UV-Visible Spectrophotometer

Your spectrophotometer is very easy to install. This section provides an

overview of the instrument and a checklist of the main installation procedure.
If you have accessories for the spectrophotometer which are not covered in
this section, see Chapter 4, “Installing Accessories” or refer to the
documentation that was delivered with your accessories for detailed
information.

Instrument overview
The front view of the spectrophotometer is shown in Figure 1 which shows the
sample compartment open. Unlike conventional instruments the UV-Visible
spectrophotometer does not suffer from ambient stray light. The open sample
area makes it easier to access it generally and to connect tubing to a flow cell
or thermostattable cell holder. The spectrophotometer is shipped with the
standard single-cell cell holder already in place. Standard and accessory cell
holders can be removed and replaced in seconds with few or no tools.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 21

2 Installation and Start Up
Installing your UV-Visible Spectrophotometer

Pushbuttons
Line power switch with
green light
Indicator

Figure 1 Front view of the Agilent Cary 8454 UV-Visible spectrophotometer

The line power switch is located at the lower left part of the instrument.
Pressing it in turns on the instrument. It stays pressed in and shows a green
light when the instrument is turned on. When the line power switch stands out
and the green light is off, the instrument is turned off.
On the front panel of the spectrophotometer is an indicator which will display
different colors depending of the actual condition of the instrument.
• Green: the instrument is ready to measure.
• Green, blinking: the instrument is measuring.
• Yellow: the instrument is in busy state, for example, turning one of the
lamps on or if both lamps are switched off.
• Red: Error condition, that is, the spectrophotometer does not pass one of
the self tests which are run when the spectrophotometer is turned on or an
error occurred during operation. In this case the UV-Visible operating
software gives a detailed error message and possible explanations are in
the online help system. Additionally the Reference Manual gives
information about troubleshooting.

22 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installation and Start Up 2
Installing your UV-Visible Spectrophotometer

• Red, blinking: Error condition of the spectrophotometer processor system.

Because in this case there is no communication with the computer there
will be no error message. The online help system and the Reference Manual
gives more information about troubleshooting.
The four pushbuttons on the front panel cause the following actions to be
performed and the resulting data being sent to the computer.
• blank: the instrument takes a blank measurement, that is, a reference
measurement of solvent without the analyte. This is used in all subsequent
sample measurements until a new blank measurement is taken. A baseline
spectrum is displayed on the computer.
• sample: the instrument takes a sample measurement or starts a series of
measurements. This depends on the parameters set in your software.
• standard: the instrument takes a measurement of a standard. Additional
information, such as concentration and so on, have to be entered in the
operating software.
• stop: the instrument and/or software aborts any ongoing activity and
returns to a to measure state.
All connections are made at the rear of the spectrophotometer, see Figure 2.

GPIB connector
and switches
Remote connector MIO slot for
LAN interface
Multicell transport
connector

GP-IO connector Line connector

RS 232 connector

Figure 2 Rear view of the Agilent Cary 8454 UV-Visible spectrophotometer

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 23

2 Installation and Start Up
Installing your UV-Visible Spectrophotometer

• The multi-cell connector allows you to connect the cable which comes from
the multicell transport.
• The GPIO (general purpose input/output) connector allow you to connect
your sipper and autosampler or other accessories.
• The remote connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
common shut down, prepare etc.
• The RS 232 connector may be used to control the spectrophotometer from a
computer through RS 232 connection, using appropriate software (for
future use). This connector needs to be activated by the configuration
switch module next to the GPIB connector. The software needs the
appropriate drivers to support this communication. See your software
documentation for further information.
• The MIO slot for the Lan interface.
• The accessory board slot is reserved for future use.
• The power input socket accepts a line voltage of 100–240 V AC with a line
frequency of 50 or 60 Hz. Maximum power consumption is 220 VA. There is
no voltage selector on your spectrophotometer because the power supply
has wide ranging capability. There are no externally accessible fuses,
because automatic electronic fuses are implemented in the power supply.
The security lever at the power input socket prevents that the
spectrophotometer cover is taken off when line power is still connected.
On the right side of the instrument there is a door for exchanging the lamps.
Behind this plastic door there is another sheet metal door. Two independent
safety light switches are implemented. They automatically turn off the lamps
when the sheet metal door is opened.

Setting up the Spectrophotometer

Connecting your Spectrophotometer via LAN to the Computer see Chapter 6,
“LAN Communication, Installation, Connection and Configuration”

Connecting Power to Your Spectrophotometer

See Figure 2 on page 23.

24 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installation and Start Up 2
Installing your UV-Visible Spectrophotometer

1 Your line voltage must be between 100–240 V AC with a line frequency of 50

or 60 Hz. There is no voltage selector on your spectrophotometer because
the power supply has automatic wide ranging capability.
2 Make sure the power switch of the spectrophotometer is in the off position,
that is, the pushbutton stands out and the LED inside the switch is off, see
Figure 1.
3 Plug the female end of the power cord into the power input socket at the
rear of the spectrophotometer. Plug the male end of the power cord into
your electrical outlet.

Installing the Waste Tubing

There is a waste outlet for the sample area. It is used to drain any liquids
which may come from the sample area in case of a leak or a spill to a waste
container. To connect your waste tubing to the spectrophotometer:
1 Lift the front part of the spectrophotometer up so that you can locate the
waste outlet underneath the sample pan.

Figure 3 Connecting the waste tubing

2 Connect the waste tubing which comes with your spectrophotometer to the
waste outlet underneath the sample pan.
3 Put the other end of the waste tubing into the waste container.
4 Make sure that the waste tubing has a constant down slope from the
spectrophotometer to the waste container.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 25

2 Installation and Start Up
Installing Your Computer

Installing Your Computer

Detailed instructions for installing your computer are specific to the type and
configuration of the computer and are provided with it. This section gives a
checklist of the main installation procedures; refer to the documentation that
was delivered with your computer for detailed information.

Setting up the computer

✔ If you have any additional accessories, install them before you set up your
computer:
• Additional memory, refer to your computer documentation.
• Additional mass storage devices (hard disk drive, tape drive, DVD), refer
to your computer documentation.
• Accessory boards excluding USB to GPIB interface, refer to your
computer documentation.
✔ Connect the keyboard and mouse to the sockets at the back of the computer.
Locate the PC keyboard and mouse for ergonomically correct access.
✔ Connect the display unit to the video socket at the back of the computer.
✔ Connect the printer cable to the socket on the printer and to the correct
printer interface socket on the back of the computer.
✔ Connect your LAN cable from the spectrophotometer to the LAN connector
on the computer.
For correct setup, see Chapter 6, “LAN Communication, Installation,
Connection and Configuration”.
✔ Connect the power sockets to the computer, display and printer, and switch
them on. Follow the instructions for setting up the computer in your
computer documentation.

26 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installation and Start Up 2
Starting the Spectrophotometer

Starting the Spectrophotometer

Before you turn on your spectrophotometer, make sure the light path through
the sample compartment is clear. All filters should be removed from the light
path. Anything blocking or absorbing part of the light beam could cause an
error indication when you turn on the spectrophotometer.
The power switch is located at the left front of the spectrophotometer. When
starting UV-Visible application software wait until the completion of the
self-test procedures for the spectrophotometer. Otherwise the application
software may not recognize the spectrophotometer.

Turning On
1 turn on your spectrophotometer with the pushbutton at the lower left part
of the instrument. The pushbutton will stay pressed in and light green if
power is present.

Indicator

Line power switch

with green light

Figure 4 Turning on the Agilent Cary 8454 UV-Visible spectrophotometer

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 27

2 Installation and Start Up
Starting the Spectrophotometer

2 The fan will start turning.

3 The indicator at the upper left corner of the front panel will light in three
different colors. The yellow color which means busy will stay on during the
start up and self test routines, until at least one of the lamps are ignited and
the spectrophotometer is ready to operate.
4 The turn on procedure, including electronic self tests, lasts about
20 seconds before the lamps start turning on. Under cold ambient
conditions the lamp may not ignite and the instrument will repeat the
ignition cycle automatically. The entire turn-on and self-test process will
take about 1–2 minutes. Optical self tests are performed which last a few
seconds.
5 When the green indicator at the upper left corner of the front panel comes
on, the instrument is ready to measure and you can turn on your computer
and printer.
If the software is set to leave both lamps off at turn on of the
spectrophotometer, the front panel indicator remains yellow.
If the indicator shows a red color, there is an error condition. Try turning
on the spectrophotometer again.
• red: error condition, that is, the spectrophotometer does not pass one of
the self-tests. In this case turn on your computer and start the UV-Visible
operating software. The software will give a detailed error message and
possible explanations are in the online help system. Additionally the
Operator’s Manual gives information about troubleshooting.
• red, blinking: error condition of the spectrophotometer processor
system. Because in this case there is no communication with the
computer there will be no error message. The online help system and the
Operator’s Manual gives more information about troubleshooting.

28 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installation and Start Up 2
Starting the Spectrophotometer

Instrument Warm-up
Technically, once your spectrophotometer has passed the self-tests it is ready
to run samples. For best results you may want to let the spectrophotometer
warm up for at least 1 hour. This time allows the optical system to stabilize
and should result in better measurements. If the instrument was not stored at
room temperature before it was turned on, allow more time for the instrument
to stabilize.
In general, the life of your spectrophotometer will be increased by leaving it on
all the time but switching off the lamps when the instrument is not in use.
lamp intensity of the deuterium lamp and lifetime of lamps decrease with use.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 29

2 Installation and Start Up
Starting the Agilent ChemStation Software

Starting the Agilent ChemStation Software

If the Agilent UV-Visible ChemStations icon is not visible on the Windows

Desktop, open the Start menu and select Agilent UV-Visible ChemStations
from the list of programs. A maximum of two sessions of an instrument can be
started in parallel; the online session must be always the first launched
session.
Two instrument icons are displayed for the Agilent UV-Visible ChemStations:
• Instrument Online starts the software in online mode (online means Agilent
ChemStation is connecting to the configured spectrophotometer).
• Instrument Offline starts the software in offline mode (offline means
Agilent ChemStation is not connecting to the configured
spectrophotometer).
To start the software, click the Instrument Online or Instrument Offline icon
in the Agilent ChemStations section.

Operational Checkout

Operational checkout is used to determine if the instrument is in proper

operating condition in combination with your UV-Visible operating software.
1 Be sure to verify the successful completion of the turn-on self test of the
spectrophotometer, that is, the indicator light on the front panel of the
spectrophotometer is lit and is green.
2 In your UV-Visible operating software, take a blank measurement and
observe the baseline noise. With an empty sample area, this should be in the
low mAU range. Excessive noise or spikes may indicate a problem.
3 For the spectrophotometer an additional (optional) qualification can be
automatically performed using your software. After warm-up of the
spectrophotometer for one hour, the instrument is ready for the automated
self-test which is located in the diagnostic portion of the software. The
successful completion of these tests complete the operational qualification
of your spectrophotometer. For reference purposes, you may want to print a
copy of the results.

30 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide

3
Good Measurement Practices
General Considerations 32
Inserting a Cell 44

This chapter describes:

• making measurements
• selecting material, optical specification and type of cell
• handling and maintaining cells
• checklist for good results
• solvents selection,
• sample preparation,
• use of filters,
• stirring and temperature control of sample,
• how to insert cuvettes into the cell holder.

Agilent Technologies 31
3 Good Measurement Practices
General Considerations

General Considerations

There are many factors that can affect the results of your measurements. This
section provides brief discussions of some of the more important ones.

Spectrophotometer Design
The sample compartment of the spectrophotometer is open. Unlike
conventional instruments, the Agilent Cary 8454 UV-Visible
spectrophotometer does not suffer from ambient false light. The open sample
area is easier to access, and it is easier to connect tubing to a flow cell or
thermostattable cell holder.

Making Measurements
Blank (Reference) and Sample Measurement
Your spectrophotometer is a single beam instrument so you must measure a
blank before you measure a sample. For high accuracy measurements, the
blank and the sample measurement should closely follow each other.
In general, a blank measurement should be repeated as often as is practical.
Even in a thermally stable environment, a blank measurement should be taken
every half hour to ensure accurate results.
Chemically, the only difference between the blank and the sample should be
the presence of the analyte(s). For measurements with liquid samples, the
blank should be a sample cell filled with the solvent you plan to use.

32 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Good Measurement Practices 3
General Considerations

Sample Cell or Cuvette Material

Quartz sample cells (cuvettes) or sample cells with quartz face plates are
required if you want to use the full 190 to 1100 nm wavelength range of your
spectrophotometer.
If you plan on working only in the visible and/or short-wave near-infrared
range of 350 to 1100 nm, you can use good quality glass cells.
Disposable plastic sample cells, for measurements in the range 400 - 1100
nm, are also available. The quality of these cells varies and they are generally
not recommended.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 33

3 Good Measurement Practices
General Considerations

Optical Specifications of Cells

The accuracy of the readings of a diode array instrument is very sensitive to
spatial shifts of the analysis light beam. Cells having non parallel opposite
faces, or so called wedge shaped cells, lead to a spatial shift of the light beam
(see Figure 5). Therefore, the opposite cell walls illuminated by the analysis
light beam have to be parallel. The degree of parallelism is measured in terms
of the angle between the two opposite cell walls. We recommend that you use
10 mm path length cells with an angle which is below 0.1 degrees of an arc.

Light Beam in

Parallel
Cell Walls

Light Beam out

Light Beam in

Non-parallel
Cell Walls

Light Beam out

Figure 5 Shift of the instrument light beam due to non parallel cell walls

34 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Good Measurement Practices 3
General Considerations

Apertured Cells or Cuvettes

In applications where sample volume is limited, apertured or microcells are
used. The width of these cells is reduced to reduce the volume and the blank
part of the cell must be blackened to avoid unwanted transmission and
reflection through the side walls. If the side walls are not blackened the result
will be poor photometric accuracy and, if different concentrations are
measured, poor linearity.
The disadvantage of apertured and microcells is that part of the light beam is
blocked. Not all the light passes through the sample and there can be some loss
in sensitivity. See Figure 6 for recommended cells and Figure 7 for cells you
should not use with the instrument.

Quartz cells Quartz cells with black apertures*

Figure 6 Recommended cells

* Quartz cells with black apertures smaller than 2 mm, when used with a multicell
CAUTION
transport, can lead to measurements of poor reproducibility.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 35

3 Good Measurement Practices
General Considerations

Quartz cells with transparent apertures,

Fluorescence cells,
Plastic cells

Figure 7 Cells you should not use with the instrument

Flow Cells
We recommend a sipper system with a flow cell for obtaining high precision
measurements. Using a flow cell eliminates the necessity of moving the cell
between blank measurement and sample measurement. Also, the cell can be
rinsed thoroughly with the solution to be measured.
The design of the flow cell should minimize entrapment of bubbles and flow
channeling to provide the most reliable results.

36 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Good Measurement Practices 3
General Considerations

Handling and Maintaining Cells or Cuvettes

Passivating New Cells
When filling a non-passivated new cell with your sample, you will observe that
air bubbles stick on the windows of your cell. To prevent the formation of
sticky bubbles, rinse the cell with cleaning and passivating fluid (part number
5062-8529). The cleaning procedure is described on the label of the cleaning
fluid container.

Cleaning Cells
The fats in fingerprints are significant absorbers in the UV region and, if left
on optical surfaces, can cause erroneous results. Wipe off all fingerprints and
contaminants before using a sample cell.
Use only high quality lens tissues (part number 9300-0761) and never dry the
inside of a cell with lens tissues. Dry the inside of the cell with pressurized,
oil free air, that prevents the cell from contamination by tissue particles, or
rinse the cell with blank or sample solution. Floating particles in the cell will
deflect the light beam and so lead to a very poor quality of the measured
spectrum.

floating particles will deflect and

scatter the light beam

Figure 8 Floating particles in a cell

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 37

3 Good Measurement Practices
General Considerations

0.01

0.00

Absorbance -0.01

-0.02

-0.03

-0.04

-0.05
200 400 600 800 1000

Wavelength [nm]
Figure 9 Spectrum taken with floating particles in the light path

Lens tissues for glasses or other uses often contain detergents or lubricants
which can affect your measurements. If possible avoid cleaning the faces of
your cell between blank and sample measurements.

Handling Cells
Always install a cell so that it faces the same direction to minimize problems
with cell non-uniformity. For best results with microcuvettes, leave your
sample cell clamped in position throughout the measurement sequence.
Solutions should be removed and replaced by pipette or use flow cells.

If glass pasteur pipettes are used, make sure that the optical windows of the cell are
CAUTION
not touched or scratched by the pipette.

38 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Good Measurement Practices 3
General Considerations

Solvents
Your choice of solvents should be based primarily on the solvent’s absorbance
characteristics over the wavelengths of interest, its suitability as a solvent for
the analyte, and on experimental conditions. Table 2 lists common solvents
and the lower limit of their useful wavelength range.

Table 2 Lower limit of UV transmission for some common solvents

Lower Limit Solvent

180–195 nm Sulfuric acid (96%)

Water
Acetonitrile

200–210 nm Cyclopentane
n-Hexane
Glycerol
2,2,4-Trimethylpentane
Methanol

210–220 nm n-Butyl alcohol

Isopropyl alcohol
Cyclohexane
Ethyl ether

245–260 nm Chloroform
Ethyl acetate
Methyl formate

265–275 nm Carbon tetrachloride

Dimethyl sulfoxide
Dimethyl formamide
Acetic acid

280–290 nm Benzene
Toluene
m-Xylene

Above 300 nm Pyridine

Acetone
Carbon disulfide

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 39

3 Good Measurement Practices
General Considerations

Many of the solvents in Table 2 are hazardous. Be sure you fully understand their
WARNING
properties before using them.

When using volatile solvents such as acetone or methylene chloride, make sure
that the sample cell is stoppered. Evaporation of a solvent can change the
solute concentration or cause solution noise due to solute convection
currents. Both of these will affect the accuracy of your measurements. We also
recommend stirring and temperature control when you use volatile solvents.
When using water as solvent we recommend using UV grade or HPLC grade
water to reduce unwanted absorbance from the water. If you are using the
sipper/sampler system the water should be degassed to avoid bubble
formation in the flow cell, especially if the water comes from a pressurized
water supply.

Sample Preparation
The sample cell should be rinsed three to five times with your intended solvent
before you fill it with the pure solvent that will be used in the measurement.
Turning the cell upside down on a small stack of absorbent tissues will help
remove any residual solvent. This treatment will minimize contamination from
previous experiments.
Samples which contain colloidal dispersions, dust or other particulate matter
should be filtered, centrifuged or allowed to settle. If not, the overall
attenuation-of-transmittance spectrum due to light scattering and/or
reflection will hide the spectral information from the analyte.

40 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Good Measurement Practices 3
General Considerations

Photosensitive Samples
A few substances are very photosensitive. They degrade or undergo
photochemical reactions if exposed to light. This can be easily seen by a
decrease of sample absorbance over time.

Use of Filters
The shorter wavelength, higher-energy UV light is most likely to degrade
photosensitive samples. If you have a problem, you can selectively block
portions of the UV spectrum with a UV cut-off filter. An optical filter wheel
assembly with three cut-off filters is available for the spectrophotometer. The
cut-off wavelength of the filter you choose should be low enough that it does
not eliminate important spectral information but high enough that it blocks
the light that could degrade your sample. If you use a filter with your samples,
you must use the same filter when you make your blank measurement.

Turning the D2-Lamp off

The short wavelength radiation leading to photodegradation comes from the
light of the D2-Lamp. For application where readings are taken at wavelengths
above 400 nm, the D2-Lamp can be turned off. The light intensity supplied by
the Tungsten lamp is sufficient for a good signal to noise ratio over the
wavelength range 400 nm - 1100 nm.When using cells with small apertures,
check the signal to noise ratio by making sample measurements under
conditions of your application.

Stirring and Temperature Control

Solution homogeneity can be a problem, especially for viscous solutions. There
are cases where, due to convection induced gradients, rapid absorbance
changes may give irreproducible data. These changes can be observed
spectroscopically by taking measurements with short integration times. To
minimize convection effects keep the temperature of your sample the same as
the cell holder or environmental temperature. Problems like these can also be
minimized by using a thermostattable cell holder and/or a stirring module.
A similar effect can occur in cases of incomplete mixing. This is especially true
where the specific gravities or miscibilities of the solvent and analyte are quite
different. Again, stirring is a way to prevent this kind of problem.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 41

3 Good Measurement Practices
General Considerations

In an unstirred cell, it is sometimes possible to observe local photodegradation

of sensitive analytes. Because the actual volume of the sample in the light path
is very small, stirring the sample will reduce the time any given analyte
molecule is in the light path. This minimizes the photodegradation and
increases homogeneity. Using a flow cell with continuous flow can yield
similar results.

Checklist for Best Results

Cell:
✔ Cell is made of quartz or glass
✔ Apertured cells have black sides
✔ Apertured cells have an aperture greater than or equal to 3 mm
✔ Cell windows are free of fingerprints and other contamination
✔ Flow cell used instead of an apertured standard cell
Measurements:
✔ Solution in cell is free of floating particles
✔ Both solution in cell, and cell walls are free of bubbles
✔ Solution in cell is mixed homogeneously
✔ Blank measured on same solvent as sample
✔ Blank measurement shows a flat baseline (Figure 10 and Figure 11 show a
good and a poor baseline)
✔ Cell orientation of blank and sample measurements is the same
✔ Ideally, the cell is not removed between the measurement, which means the
cell is filled/rinsed using a pipette or a flow cell is used
✔ Time between blank and sample measurement should be short

42 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Good Measurement Practices 3
General Considerations

200 400 600 800 1000

0.0015

Absorbance 0.0010

0.0005

0.0000

-0.0005

-0.0010

200 400 600 800 1000

Wavelength [nm]
Figure 10 Example of a blank on water showing a good baseline

0.01

0.00
Absorbance

-0.01

-0.02

-0.03

-0.04

-0.05
200 400 600 800 1000

Wavelength [nm]
Figure 11 Example of a blank on water with bubbles causing a poor baseline

If your blank or spectra show artifacts similar to the one in Figure 11, see “Solvents” on
NOTE page 39 to optimize the measurement procedure.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 43

3 Good Measurement Practices
Inserting a Cell

Inserting a Cell
Your spectrophotometer is shipped with the standard single-cell cell holder
you must first install in the sample compartment. This cell holder
accommodates standard cells or flow cells. To insert a sample cell (cuvette) in
the cell holder:

1 Move the locking lever to its up position. 2 Insert the sample cell, making sure you orient it
correctly. The frosted (non-clear) sides of the
sample cell should not be in the path of the light
beam.

3 Lock the sample cell in place by pushing the Small volume flow cells and particularly any cells
locking lever back down. with less than a 2 mm aperture may require use of
the optional adjustable cell holder. The adjustable
cell holder helps you ensure the cells are properly
centered in the light path.

44 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide

4
Installing Accessories
Thermostattable Cell Holder 46
Cell Stirring Module 48
Long Path Cell Holder 52
Optical Filter Wheel 55

Many simple, mechanical/optical accessories are available to expand the

capabilities of your UV-Visible spectrophotometer. This section briefly
describes these accessories and outlines how to install them.
The following more complex accessories with electronic components are
available to enhance the functionality and capabilities of your
spectrophotometer. Information about these are included with the accessories
in separate handbooks.
• Sipper system
• Autosampler
• Multicell transport
• Peltier temperature control accessory

Agilent Technologies 45
4 Installing Accessories
Thermostattable Cell Holder

Thermostattable Cell Holder

The Agilent 89054A thermostattable cell holder (see Figure 12) is a single-cell
cell holder with a manifold around the cell. Water from a thermostatted water
bath can be circulated through the manifold to hold the sample cell at a
uniform temperature. Up to 1 cm open-topped cells and flow cells can be used
in a thermostattable cell holder.

Tubing connector

Tubing restraint tab

Figure 12 Thermostattable cell holder

46 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installing Accessories 4
Thermostattable Cell Holder

Installing a Thermostattable Cell Holder

1 Loosen the hold-down screws and remove the current sample holder from
the sample compartment.
2 Orient the thermostattable cell holder the same way the standard cell
holder was oriented and lower it onto the sample compartment mounting
guides.
3 Tighten the hold-down screws.
4 Connect tubing (silicon, 3/16 inch i.d.) to the inlet and outlet of the
manifold. It does not matter which side is used as the inlet and which side
is used as the outlet. If necessary, route the tubing under the tubing
restraint tab to keep it from interfering with the light beam. Make sure the
tubing is securely attached; it expands when heated and if it is not secure, a
leak will result.
5 Insert the sample cell and lower the lever down to lock the cell in place.
6 Begin pumping thermostatted water through the manifold. Wait for the
sample cell and sample to reach thermal equilibrium before you make any
measurements. The time required to reach thermal equilibrium will vary
depending on the water temperature and the sample but generally should
take about five minutes.
When the thermostattable cell holder is not in use, it is best to disconnect the
tubing and drain the manifold.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 47

4 Installing Accessories
Cell Stirring Module

Cell Stirring Module

The Agilent 89055A cell stirring module shown in Figure 13, mounts inside the
base of the thermostattable cell holder. It enables magnetic induction stirring
inside a standard 1 cm sample cell. The cell stirring module contains a
plastic-encased magnetic impeller that can be driven by water or pressurized
air. When you put a magnetic stirring bar (9301-1161) into the filled sample
cell, the stirring bar rotates in tandem with the impeller magnet, thus stirring
the sample.

Figure 13 Cell stirring module

48 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installing Accessories 4
Cell Stirring Module

Installing the Cell Stirring Module

1 Remove the thermostattable cell holder from the sample compartment.
2 Remove the three screws that hold the thermostattable cell holder to its
base. Remove the cell holder and turn it upside-down.
3 Insert the cell stirring module into the cavity in the base of the
thermostattable cell holder, see Figure 14.

Figure 14 Installing the cell stirring module

4 Tighten the four screws (flathead, M36 mm) that hold the cell stirring
module in place.
5 Attach the thermostattable cell holder to its base.
6 Mount the thermostattable cell holder back in the sample compartment.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 49

4 Installing Accessories
Cell Stirring Module

Tubing Connections and Operation

The cell stirring module can be driven by either water or air. Water is
preferable because it is more easily controlled. Water temperature can be up to
100 C (212 F) with a maximum allowable back pressure of 4 bar
(approximately 60 psi). If air is used to drive the cell stirring module, the
pressure should be less than 0.3 bar (approximately 5 psi) when the stirrer is
started. Again, the maximum pressure is 4 bar (approximately 60 psi).
There are several ways you can connect a water or air supply to the cell
stirring module, see Figure 15. In all cases we recommend using 3/16 inch i.d.
silicon tubing between the water supply and the stirring module.
• The stirring module can be used alone, without the thermostattable
functions of the cell holder.
• The same water supply can be used to regulate sample cell temperature and
to power the cell stirring module.

50 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installing Accessories 4
Cell Stirring Module

If you need a faster stirring speed than can be obtained by connecting the
manifold and cell stirring module in series, you can connect separate supplies
to the manifold and stirring module.

B
A
D
C

Figure 15 Tubing connections for a cell stirring module

Table 3 Tubing connections for a cell stirring module

Connection Comment

A (in), B (out) Without stirring

C (in), D (out) Without cell thermostatting

A (in), B to D, C (out) In series with cell thermostatting

A and C (in), B and D (out) In parallel with cell thermostatting

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 51

4 Installing Accessories
Long Path Cell Holder

Long Path Cell Holder

The Agilent 89076A long path cell holder is a sample cell holder which can be
adjusted to hold most cylindrical and rectangular sample cells with path
lengths of up to ten centimeters. It uses the same mounting system as the
other sample cell holders.

Cell stops

Figure 16 Long path cell holder

The long path cell holder includes built-in cell stops for accurate positioning of
1, 2, 5, and 10-cm sample cells.
The optical filter wheel (08451-60302) can be mounted on the long path cell
holder in the same way it is mounted on other cell holders, see “Optical Filter
Wheel” on page 55 for details. However, instead of a screwdriver, a 2.5-mm hex
wrench is required to install the optical filter wheel on the long path cell
holder.

52 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installing Accessories 4
Long Path Cell Holder

Installing the Long Path Cell Holder

1 Loosen the hold-down screws and remove the current sample holder from
the sample compartment.
2 Orient the long path cell holder so that the clamps that hold the sample cell
in place are on the right. Lower the cell holder onto the sample
compartment mounting guides.
3 Tighten the hold-down screws.

Installing a Sample Cell

1 Position the cell stops according to the path length of the sample cell you
will be using. This example shows a 1-cm sample cell:

Cell stops
Cell clamps
Position cell here

Clamp screws

Figure 17 Positioning the cell stops for a 1-cm sample cell

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 53

4 Installing Accessories
Long Path Cell Holder

2 If your sample cell is not one of the standard lengths, position the cell stops
as for a long path sample cell, i.e. move all cell stops in Figure 17 to the left.
3 Because the light beam is collimated, absolute positioning of the sample cell
is not important; however, consistent cell positioning gives more consistent
results. The cell stops make it easier to consistently position your sample
cells.
4 Install the sample cell between the clamps. Slide it against the cell stop. If
your sample cell is not one of the standard lengths, position it so the clamps
are gripping the mid-point of the cell.
5 Allow the clamps to close against the sample cell, see Figure 18. Tighten the
four screws that hold the sample cell in place.

Figure 18 Installed 10-cm cylindrical cell in the long path cell holder

54 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installing Accessories 4
Optical Filter Wheel

Optical Filter Wheel

To achieve optimum measurement conditions, optical filters may be necessary

for spectral measurements of photosensitive samples. Agilent Technologies
offers an optical filter wheel assembly (08451-60302), see Figure 19, which can
be mounted on the standard cell holder, the adjustable cell holder or the
thermostattable cell holder. An optical filter wheel with the same filters but
with a different mounting can be used with the multicell transport, see the
multicell transport User’s Guide.

Notches indicate filter number

Figure 19 Optical filter wheel assembly

The filter wheel rotates to four different positions. Position zero is empty and
permits 100% transmittance at all light wavelengths. Positions one, two and
three have filters which absorb varying amounts of the UV spectrum.
Figure 20 shows the transmission characteristics of each filter.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 55

4 Installing Accessories
Optical Filter Wheel

0
100

80
1
60

40 2

20 3

190 200 300 400 500 600 700 800 820

Figure 20 Transmission characteristics of filter wheel filters

Table 4 Filter wheel positions

Position Filter

0 None

1 UV roll-off

2 265 nm UV cut-off (at 50%T)

3 295 nm UV cut-off (at 50%T)

The choice of when to use a filter and which filter to use should be made only
after considering all the factors that affect your measurements. If you decide
that a filter is needed, you should choose a filter that transmits the
wavelengths needed for analytical information and that blocks the
wavelengths that contribute to sample degradation. Often, your final choice
will be a compromise since sometimes even the light at the wavelengths of
analytical interest can affect your sample.
If you do not need a filter for a group of measurements, you can move the filter
wheel to position zero. This permits full light transmission. When you will not
be using the filter wheel for an extended period of time you should remove the
filter wheel assembly from the cell holder and store it in a clean plastic bag.
The optical filters of the filter wheel must be kept clean. Dirty filters interfere
with light transmission and lower the spectrophotometer performance. These
filters may be cleaned with isopropanol and photographic lens tissues.

56 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Installing Accessories 4
Optical Filter Wheel

Installing the Filter Wheel Assembly

Installing the filter wheel assembly is fairly simple. The procedure is the same
for all three cell holders. You will need a Phillips screwdriver to install the
filter wheel assembly. However, if you install the filter wheel on the long path
cell holder, instead of a screwdriver, a 2.5-mm hex wrench is required.
1 Remove the cell holder from the sample compartment.
2 Remove the two screws shown in Figure 21.
3 Position the filter wheel assembly clamp as shown in Figure 21. Replace the
two screws but do not tighten them.

Screw

Clamp

Figure 21 Installing the optical filter wheel assembly

4 Tilt the outer edge of the clamp up slightly.

5 Bring the filter wheel assembly against the cell holder so that the clamp fits
in the slot of the filter wheel assembly. The mounting ridges on the filter
wheel assembly should fit into the holes in the clamp. The base of the filter
wheel assembly should be against the base of the cell holder.
6 Tighten the screws that hold the clamp.

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4 Installing Accessories
Optical Filter Wheel

Removing the Filter Wheel Assembly

1 Loosen but do not remove the two screws that hold the clamp.
2 Separate the filter wheel assembly from the cell holder.
3 The filter wheel clamp may be left in place or removed. In either case,
retighten the two screws.
4 Store the filter wheel in a clean plastic bag.

58 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide

5
Installing the UV-Visible Software
Agilent ChemStation Computer Details 60
Installing the Agilent ChemStation Using the DVD 63

This chapter describes the minimum hardware and software requirements

and describes the installation process for the UV-Visible software.
It is assumed that a supported Microsoft Windows operating system is already
installed.

Agilent Technologies 59
5 Installing the UV-Visible Software
Agilent ChemStation Computer Details

Agilent ChemStation Computer Details

This section describes the PC hardware and operating system requirements

that must be met for successful installation and operation of Agilent
UV-Visible ChemStation.
Agilent UV-Visible ChemStation is supported on personal computers with a 3
GHz 64-bit (x86) processor or better. All PC hardware and peripherals must be
listed in the Microsoft Windows Marketplace Tested Products List Hardware
section. The hardware testing status is available from the Microsoft home page
on the world wide web: (http://winqual.microsoft.com/HCL/Default.aspx). If
your PC hardware is not tested, we cannot guarantee that the system will work
with the supported operating system.

The software will run with the minimum amount of RAM installed, but system performance
NOTE will depend on what other processes are running.

Minimum PC Requirements for Agilent ChemStation

Table 5 Minimum PC Requirements

PC configuration Windows 7 Professional 64-bit (SP1)

Windows 10 Professional 64-bit

Graphics resolution The recommended graphics resolutions for video

graphics adapter are:
1024 x 768 with large fonts or
1280 x 1024 with large fonts

Processor 3 GHz

Screen resolution Super VGA (1280 × 1024)

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 Installing the UV-Visible Software 5
Agilent ChemStation Computer Details

Table 5 Minimum PC Requirements (continued)

Mass storage 40 GB Hard-Disk and CD-ROM

Memory Minimum of 4 GB of RAM for UV-Visible

ChemStation software and a single instrument.

The minimum recommended amount of memory

for a single- bath dissolution system (G1118AA)
and kinetic measurements (G1117AA) is 3 GB of
RAM.
Add 256 MB of RAM for each additional bath in a
multi-bath dissolution system (G1118AA).

In all cases, an appropriate amount of virtual

memory should be configured.

A maximum of four instruments may be interfaced to each Agilent ChemStation.

NOTE

If you are upgrading from a previous version of Agilent ChemStation, you must uninstall the
NOTE old version of ChemStation first.

Scheduled Processes
Some scheduled processes, like virus checking or defragmenting, may cause
instrument communication problems. Due to these problems, any automated
measurement will be aborted. To prevent problems of this kind please disable
any scheduled event.

Number Format
To avoid wrong values displayed or printed, the International settings must
include the correct number format. Set the Number Format Decimal Separator
to "." (decimal point) in Control Panel > Region and Settings.

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Agilent ChemStation Computer Details

Printer
Logon to the PC with the ChemStationSystemUser account and then make
sure that a default printer is configured in your Microsoft Windows operating
system. This is done via the Control Panel application.
If no default printer is configured, you may encounter the following problems:
• the 'Copy To Clipboard' menu may have an error
• in the 'Custom Report View' there may be problems with new templates
• the 'Print Preview' will not work

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 Installing the UV-Visible Software 5
Installing the Agilent ChemStation Using the DVD

Installing the Agilent ChemStation Using the DVD

Overview
This section explains how to use the Agilent Technologies UV-Visible
ChemStation Software Products DVD to:
• install the Agilent ChemStation for the first time
• upgrade/repair an existing Agilent ChemStation software
• install an additional Agilent ChemStation module

Local Administrator rights on the target PC are required to perform the installation.
NOTE

Uninstalling Pre-existing Agilent UV-Visible ChemStation Software

To remove all analytical ChemStation software from your hard disk for
versions B.05.02 and earlier:
1 Insert your Agilent Technologies UV-Visible ChemStation Software DVD
into the drive.
2 Run the setup program on the DVD.
3 Select Remove and then click Next.
Note that the removal of ChemStation software can also be done from within
the Control Panel.
To remove all analytical ChemStation software from your hard disk for version
B.05.03 ensure the following conditions are met and then follow the procedure
above to uninstall the software.
1 Be logged in as a local administrator such as ChemStationSystem.
2 Have Full Control access to the following:
• C:\Chem32
• C:\Chem32\core

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Installing the Agilent ChemStation Using the DVD

• C:\Chem32\uvexe
• C:\Chem32\1
• C:\Chem32\2
• C:\Chem32\3
• C:\Chem32\4
• C:\Chem32\TMP
• C:\Chem32\NonInst
• C:\Chem32\ChemStation.ini

Only files installed by the product DVD are removed. All additional files such as method and
NOTE data files are not automatically removed. By default they are located in the respective
instrument directory C:\Chem32\1.

If the Security Pack had been installed, please see your manual Agilent ChemStation
NOTE Security Pack for UV-Visible Spectroscopy - User's Guide for details.

During the installation some of your configuration files will be changed. If you
don't intend to use any ChemStation software you can remove the
ChemStation.ini file located in the operating system folder, C:\Windows by
default.

Installation Procedure
Use the following procedure if you are using the DVD to install or upgrade the
Agilent ChemStation software.
Before installing the software:
• Set up the computer hardware.
• To connect your Spectrophotometer via LAN to the Computer see
Chapter 6, “LAN Communication, Installation, Connection and
Configuration”

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Installing the Agilent ChemStation Using the DVD

• Make sure an operating system, supported by your revision of the Agilent

UV-Visible ChemStation software, is available on the target PC.

If ChemStation is already installed and you are only adding a new module (also called an
NOTE Add-on), you must ensure the following conditions are met before following the procedure
below. If you are installing ChemStation and all modules at the same time, skip to the
installation procedure.

Prerequisites to Install a New Module to Existing ChemStation Software

1 Be logged in as a local administrator such as ChemStationSystem.
2 Have Full Control access to the following:
• C:\Chem32
• C:\Chem32\core
• C:\Chem32\uvexe
• C:\Chem32\1
• C:\Chem32\2
• C:\Chem32\3
• C:\Chem32\4
• C:\Chem32\TMP
• C:\Chem32\NonInst
• C:\Chem32\ChemStation.ini

Installing or Upgrading ChemStation

1 Insert the Agilent UV-Visible ChemStation Products DVD into the DVD
drive.
2 Click the Search or Cortana icon in the Windows 10 taskbar or Windows 7
Start menu. Type “Run”. Select Run.
3 At the command line, type diskdrive:\Setup.exe (for example, D:\
Setup.exe), then click OK.

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4 Read the licence agreement and click Yes to accept.

5 Accept the default installation path C:\Chem32, then click Next.

Agilent ChemStation must be installed in the default location C:\Chem32 directory.

NOTE

6 Select the additional modules you want to install and click Next.

Note that the Security-Pack Add-On and the ECM Add-On are mutually
exclusive. One or the other can be installed, but not both.

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Installing the Agilent ChemStation Using the DVD

7 Enter the License Registration Number for each selected module in the
entry line and click Add License. When all required licenses are added,
press NEXT.

The applicable product and license numbers are printed on your Software
Certificate and Registration Packet (see the example license registration
label).

SOFTWARE REGISTRATION LABEL FOR

PRODUCT NUMBER
G1115AA

P LE REVISION CODE
A.08.01
S AM REGISTRATION
NUMBER
DU08E7777A

Figure 22 Example license registration label

8 The configuration editor will open automatically as part of setup. Adjust

the configuration if desired. Save the configuration and exit the
configuration editor. Setup will then continue.

Editing the instrument configuration in the Configuration Editor must be performed by a

NOTE user logged onto the client PC as the administrator account, ChemStationSystem.

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Installing the Agilent ChemStation Using the DVD

9 Click Finish to complete the setup and reboot your system.

Restarting your system is required before you proceed.
NOTE

If you selected the G3770AA or G5182AA ECM access module, establish your ECM access
NOTE using Internet Explorer before you first launch UV-Visible ChemStation.

During installation an administrator account ChemStationSystem is added to the PC. This

NOTE administrator is created with a default password: ChemStation!1!System. If this password
does not meet the current password policy requirements then the installation will fail. To
proceed, temporarily turn off password requirements on the PC until the installation has
successfully completed and then change the ChemStationSystem password as required.

Administration of the ChemStationSystem Administrator Account

Administration of the ChemStation administrator (ChemStationSystem)
includes the following activities when the workstation is a PC on a domain:
1 Create a domain user named ChemStationSystem and make this user a
local administrator.
2 Give (NFTS-Permission) Full Control for ChemStationSystem on the
following directories and files (see below for instructions):
• C:\Chem32
• C:\Chem32\core
• C:\Chem32\uvexe
• C:\Chem32\1
• C:\Chem32\2
• C:\Chem32\3
• C:\Chem32\4
• C:\Chem32\TMP
• C:\Chem32\NonInst
• C:\Windows\ChemStation.ini

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3 Run “ChemStation System Configuration” from the Start Menu.

4 In the Save UV-Vis ChemStation System User Password window, enter and
confirm the Microsoft Windows password for the ChemStationSystem
account and then click on Save. For a local installation the domain listed
should be the computer name.

Figure 23 Save UV-Vis ChemStation System Configuration changes

If these details are not correct, you will not be able to start ChemStation.
CAUTION

5 Ensure that the dialog “User Configuration Saved” is shown.

6 Log out of Microsoft Windows.
7 Log into the ChemStationSystem account. Set up any printers that are to be
used with the UV-Visible ChemStation while on this account. Log out when
finished.

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Designating Folder Permissions when Creating a Domain ChemStation User

1 Right click on the file/folder and then select Properties.

Figure 24 Accessing the folder properties

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Installing the Agilent ChemStation Using the DVD

2 Select the Security Tab and then click Edit.

Figure 25 Opening the Edit Security dialog

3 Click Add.
4 Make sure the location is the PC, and type ChemStationSystem in the object
name field, click OK and click OK again.

Figure 26 Entering the object name

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5 Highlight the ChemStationSystem domain user that was just added and
then select Full Control and click OK.
6 Repeat this for all of the folders described in the section above.

Configuring Your System

The setup program configures the system automatically for your UV-Visible
spectrophotometer as Instrument 1, with an GPIB address of 25.
If you wish to use LAN communication, set up an additional
spectrophotometer, or set up an temperature control accessory you must
configure the system.
To configure your system:
1 In the Agilent ChemStation group start the Agilent UV-Visible
Configuration Editor.
2 Choose Instruments from the Configure menu.
• The Instrument Type box lists the instrument types available on your
Agilent ChemStation. Select one to begin your configuration.
• Enter the name you want to call the instrument in the Instrument Name
box. The Agilent ChemStation will use this name to identify the
instrument.
• Select Normal under Initial Screen Window Size to have your
instrument session window appear as a full screen but with an open area
across the bottom for icons. If you select Icon or Full Screen, the
instrument session window will start as an icon or as a full screen.
• Click OK to enter your selections and to display the Instrument Name -
Device Configuration.
• To modify settings in an existing instrument configuration, you must
select the module first in the Selected Modules frame and press the
Delete button.
• Select the module in the Modules frame and chose the type of
interfacing.
• In case of a LAN connection, select the LAN option and press Add. In the
upcoming dialog select Identify by Host Name or Identify by IP Address
and enter the selected information - Host Name: or IP Address: - in the
entry field. Press OK to add the module to the list of Selected Modules.

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Installing the Agilent ChemStation Using the DVD

• Click OK to complete the instrument configuration.

If you configure more than one instrument, you must ensure that each
instrument has a unique LAN address. You can configure up to four
spectrophotometers.
3 Click OK to enter your selections and to return to the main menu.
4 If you want to change the color of a screen element:
Choose Colors from the Configuration menu, select that element under
Screen Elements, then select a color from those shown below in the
Standard Colors or Custom Colors list box. Your changes will appear in the
Screen Elements list box. You can create custom colors and add these, use
the Add Custom Colors... button and follow the instructions available with
the Help.
5 If you want to add new instruments:
Choose Add New Instrument from the Add/Delete menu to add a new
instrument window to the Configuration Editor screen. Follow the above
procedure to configure the instrument. Choose Delete Instrument from the
Add/Delete menu to remove an instrument from the configuration.
6 Choose Save from the File menu to save your configuration.
7 Choose Exit from the File menu to close the Configuration Editor.
8 To set parameters for the accessories you have installed and configured,
refer to the Help in the UV-Visible ChemStation software.

Autosamplers
If you have an Agilent XY autosampler, your Agilent ChemStation software
requires a special driver and a cable. See the readme.txt for further
information.

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 Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide

6
LAN Communication, Installation,
Connection and Configuration
Installing and Configuring TCP/IP on the PC 76
Installing the LAN Interface in your UV-Visible Spectrophotometer 79
Connecting the Spectrophotometer to the PC 80
Using the Agilent BootP Service 82
Using the Agilent ChemStation Configuration Editor to Configure the
Spectrophotometer 91
Troubleshooting LAN Communication 94

The first five sections of this chapter describe all necessary installations,
connections and configurations of the LAN communication.
The last section of this chapter helps you if the LAN communication does not
work properly.

Agilent Technologies 75
6 LAN Communication, Installation, Connection and Configuration
Installing and Configuring TCP/IP on the PC

Installing and Configuring TCP/IP on the PC

Installation of the TCP/IP protocol

1 For Windows 7, select Start > Control Panel> Network and Sharing
Center. For Windows 10, right-click on Start and then select Control Panel
> Network and Internet > Network and Sharing Center.
2 In the Tasks list click on Manage network connections. In the LAN or
High-Speed Internet list right click on your LAN adapter icon and select
Properties.

Figure 27 Local Area Connection Properties

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 LAN Communication, Installation, Connection and Configuration 6
Installing and Configuring TCP/IP on the PC

3 In the ‘Local Area Connection Properties’ dialog (see Figure 27) click
Configure... to make sure the device is working properly.

If the device is not working properly or no network interface card is configured, please refer
NOTE to the hardware manual of your network card and the PC for how to install and configure a
network interface card.

4 In the ‘This connection uses the following items:’ section of this dialog make
sure the ‘Internet Protocol Version 4(TCP/IPv4)’ is available and checked.

If the TCP/IP protocol is not installed, use the Install button, select Protocol from the list of
NOTE network components to install and press Add.
From the ‘Manufacturers:’ select ‘Microsoft’ and from the ‘Network Protocol:’ select the
‘Internet Protocol Version 4(TCP/IPv4)’ and press ‘OK’. If required, reboot your computer.

5 Select the ‘Internet Protocol Version 4(TCP/IPv4)’ and click Properties.

If the PC is connected to your companies LAN, do not change any setting. Note down the
NOTE ‘Subnet Mask’ if available. Ask your responsible IT consultant for this mask if not available
and request a fixed IP address for your instrument, if you want to use the existing LAN for
the instrument connection.
For further details please see“Connecting the Spectrophotometer to the PC”.

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6 LAN Communication, Installation, Connection and Configuration
Installing and Configuring TCP/IP on the PC

Figure 28 Internet Protocol Version 4(TCP/IPv4) Properties

6 In the ‘Internet Protocol Version 4 (TCP/IPv4) Properties’ dialog (see

Figure 28) select the option Use the following IP address and enter your
computer’s IP Address and Subnet Mask.
a If the system is on an isolated LAN, the following defaults can be used:
192.168.254.10 for the PC IP address, and 255.255.255.0 for the subnet
mask. (note: to use these defaults, DIP switches 5 and 6 on the LAN card
must be "ON").

Gateway, DNS and WINS IP addresses do not need to be configured if you do not connect
NOTE to any other part of a network.

7 Turn off the Microsoft Windows Firewall and reboot the computer.

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Installing the LAN Interface in your UV-Visible Spectrophotometer

Installing the LAN Interface in your UV-Visible

Spectrophotometer

The JetDirect card (G1846A) and the TalkToLab card (G1369C) are the LAN
interfaces used to physically connect the spectrophotometer to the PC via a
LAN cable. To install the LAN interface card:
1 Switch off the spectrophotometer.
2 Remove the metal cover at the upper right of the rear panel.
3 If the system is on an isolated LAN, the following defaults can be used:
192.168.254.10 for the PC IP address, 255.255.255.0 for the subnet mask,
and 192.168.254.11 for the spectrophotometer. (note: to use these defaults,
DIP switches 5 and 6 on the LAN card must be "ON").
4 Plug the LAN interface card into the slot and screw down the two mounting
screws.

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6 LAN Communication, Installation, Connection and Configuration
Connecting the Spectrophotometer to the PC

Connecting the Spectrophotometer to the PC

This section describes how to setup the connection between your

instrument(s), and the PC. If you are going to connect to an existing LAN,
please contact your system administrator for further assistance.

Single Instrument Installation

In a single instrument installation, only one UV-Visible spectrophotometer is
connected to the PC’s LAN interface. In this configuration, only the black
10 ft. long crossover twisted pair cable (part number 5183-4649) can be used.
This is supplied with the Agilent UV-Visible ChemStation software.

Do not use the standard (non-crossover) Ethertwist 10BaseT cable supplied with the LAN
NOTE interface card G1846A for direct single instrument connection.

Multiple Instrument Installation

If you want to connect more than one spectrophotometer to the
Agilent ChemStation PC or if the PC needs to connect to a network using the
same network connection, a switch (for example the G2402A 8 port switch) is
required. The G2402A switch comes with one standard EtherTwist 10BaseT
cable that is used for the connection to the PC. Use the standard EtherTwist
10BaseT cable (part number G1530-61485) provided with the LAN interface
cards to connect the switch to the spectrophotometer.

Do not use the black 10 ft. long crossover twisted pair cable (part number 5183-4649) in an
NOTE installation with multiple instruments.

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Connecting the Spectrophotometer to the PC

Use standard twisted

pair LAN cables for the
connections.

Personal computer Network Switch

Instruments

Figure 29 LAN connection using a switch

Setting the IP Address of the Spectrophotometer

The IP address of the LAN interface card inserted to the spectrophotometer
can be assigned by using the Agilent Bootp service. This service assigns the
LAN interface card with a configured IP address, each time an IP address is
requested. Refer to the next section “Using the Agilent BootP Service” on
page 82.

If the system is on an isolated LAN and the default IP address 192.168.254.11 for the
NOTE spectrophotometer is used, BootP is not necessary.

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6 LAN Communication, Installation, Connection and Configuration
Using the Agilent BootP Service

Using the Agilent BootP Service

What is the Agilent BootP Service?

If the system is on an isolated LAN and the default IP address 192.168.254.11 for the
NOTE spectrophotometer is used, BootP is not necessary.

The Agilent Bootstrap Protocol Service, acronym BootP Service, provides

central administration of IP addresses for Agilent instruments on a LAN. The
service runs on the instrument LAN PC, which must be running TCP/IP
network protocol and cannot run a DHCP server/service or another BootP
Service.
When an instrument is powered on, an Agilent LAN interface card in the
instrument broadcasts a request for an IP address or Host Name and provides
its hardware address as an identifier. The request may continue for up to 5
minutes. The Agilent BootP Service answers this request and passes a
previously defined IP address and Host Name associated with the hardware
address to the requesting instrument.
In case of the instrument on the LAN, the G1369C LAN card used to connect
the spectrophotometer to the LAN does not store any settings, so each time
the instrument is powered on the card sends a request to the network for an IP
address and setting information. The implementation of the BootP protocol on
the G1369C LAN card conforms to RFC 951 and RFC 1048.
In order to provide this information to the card, the BootP Service must be
operational before the card sends the request and the BootP Service must be
configured to know the hardware address (also called Media Access Control,
acronym MAC address) of the LAN interface card. Multiple LAN interface cards
can be configured by means of a single BootP Service.
If your network is already using a BootP Service, please refer to section
“Configuring the G1369C LAN Card Using BootP” on page 89. If you do not
have a BootP Service, we suggest to use the Agilent BootP Service program
supplied on the Agilent UV-Visible ChemStation DVD.

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Using the Agilent BootP Service

Addresses
Before installing and configuring BootP Service, you must know the IP
addresses of the computer and instruments, the subnet mask, and the
gateway.

Table 6 Example

Device Address

PC 10.10.10.1

Spectrophotometer 10.10.10.101

Subnet mask 255.255.255.0

Gateway 10.10.10.1

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6 LAN Communication, Installation, Connection and Configuration
Using the Agilent BootP Service

Using the Agilent BootP Service Program

The Agilent BootP Service program allows configuring the LAN interface card.
Within the Launch Manager of the program you can easily add a MAC address
and IP address pair to the configuration. The Launch Manager is displaying a
list of the currently configured MAC address and allows modification of the
current configured settings.

Figure 30 Configured interfaces view of the Agilent BootP Service

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Using the Agilent BootP Service

Configure the Agilent BootP Service Program

If the system is on an isolated LAN and the default IP address 192.168.254.11 for the
NOTE spectrophotometer is used, BootP is not necessary.

Before configuring BootP Service, you must know the IP addresses of the
computer and instruments, the subnet mask, and the gateway. The MAC or
hardware address of a LAN interface is a world wide unique identifier. No
other network device will have the same MAC address. The MAC address is
printed on the card or can be read out during the configuration procedure:

If the PC has been rebooted after installing the Agilent BootP Service, the Agilent BootP
NOTE Service is started automatically during the next startup of the system. To change BootP
settings, the service must be stopped, the changes made, then restarted. The details are
documented within this section.

Determine your MAC Address

Determine the MAC address of the LAN card installed. The number is
engraved in the board.
Write down the MAC address before installing the card into the module. To
remove the card from the system, turn the module off before removing the
LAN card. Read the MAC address from the label. Reinstall the card and turn
on the module again.

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6 LAN Communication, Installation, Connection and Configuration
Using the Agilent BootP Service

Using the Launch Manager

1 To add the instrument to the network go to Start > All Programs or All
Apps > Agilent BootP Service>Edit BootP Settings. The BootP Settings
screen appears.
2 Uncheck Do you want to log BootP requests?
3 Click Launch Manager. The BootP Manager screen appears.

Figure 31 Configured interfaces view of the Agilent BootP Service

4 Click Add.... The Add BootP Entry screen appears.

5 Make the following entries for your instrument:
• MAC Address
• Host Name
• IP Address
• Comment, if desired
• Subnet Mask
• Gateway Address
6 Click OK.
7 Exit Launch Manager and reboot both the PC and the spectrophotometer.
8 Ping the IP address from a command prompt on the PC to verify.

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Configure the Agilent BootP Service

BootP Service starts automatically when your PC reboots. In order to add an
additional instrument or to change the configuration the service must be
stopped. After the changes have been applied, the service must be restarted.
1 To stop the BootP service:
For Windows 7: Start > Control Panel > System and Security >
Administrative Tools >Services.
For Windows 10: Right-click on Start and then select Control Panel >
System and Security > Administrative Tools > Services.

Figure 32 Services window

2 Right click Agilent BootP Service.

3 Select Stop.
4 Close the Services and Administrative Tools windows.
5 To edit the BootP Settings go to Start >All Programs or All Apps > Agilent
BootP Service > Edit BootP Settings. The BootP Settings screen appears.

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When this screen opens the first time, the default settings from installation
are displayed.
6 To edit the TabFile, select Maintain bootp tabfile?
The default tab file was created at installation and is located at Program
Files\Common Files\Agilent Shared\BootP\bin\TabFile. It contains
configuration information entered on this screen.

If necessary you can

• change the BootPtab File Location by using the browse button on the
right (a valid BootP tabfile must exist).
• create your own template for the tabfile by selecting Create template
BootP tabfile? and click Create Template.
7 Select Do you want to log bootp requests?
The default log file was created at installation and is located at Program
Files\Common Files\Agilent Shared\BootP\bin\LogFile. It contains an
entry for every time a device requests configuration information from
BootP.

If necessary you can change the BootPlog File Location by using the browse
button on the right (a valid BootP tabfile must exist).
8 Perform your necessary changes by e.g. creating a log file entry of a new
instrument and edit the new instrument using the Launch Manager.
9 Deselect Do you want to log bootp requests?
10 Click OK to save the values or Cancel to discard them. The program ends.
11 To restart the BootP Service:
For Windows 7: Start > Control Panel > System and Security >
Administrative Tools >Services.
For Windows 10: Right-click on Start and then select Control Panel >
System and Security > Administrative Tools > Services.
12 Right click Agilent BootP Service.
13 Select Start.
14 Close the Services and Administrative Tools screen.
15 This completes the configuration.

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Configuring the G1369C LAN Card Using BootP

In order for an internal G1369C LAN card to use BootP, the default setting:
bootp=YES must be set. For more details please see the user manual of your
G1369C LAN interface card.
To change the setting, the card must be reset; this is done each time the
instrument with the G1369C LAN card inserted is power cycled.
The Agilent BootP Service uses a file called bootptab were all the
configuration information is stored. The BootP Service program on the
Agilent ChemStation CD-ROM allows easy editing of the bootptab file. If a
BootP Service is already installed, the following information must be supplied
to the system administrator to be able to correctly set up the bootptab file.

# CAG Bootptab file

# global defaults
global.defaults:\
sm=255.255.255.0:\
gw=<gateway address - ask your system administrator>

#8454 lab
UV-Vis:\
ht=1:\
ha=0030D30D3277:\
ip=<ip address - ask your system administrator>
sm=255.255.255.0:\
gw=<gateway address - ask your system administrator>
vm=auto:\
hn:\
bs=auto:\
T145=64:T146=01:T147=01

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Using the Agilent BootP Service

The entries are described below

• #8454 lab
Comment line starting the configuration set for an Agilent Cary 8454
UV-Visible spectrophotometer. A comment is preceded with #; all
characters after the # on the same line are ignored by the BootP Service.
• UV-Vis:\
Host name of the instrument. The host name communication can only be
used if a DNS server (Domain name server) is correctly set up for the name
or a corresponding entry in the HOSTS file exists (windows\system32\
drivers\etc\hosts).
• ha=0030D30D3277:\
This is the hardware or MAC address of the G1369C LAN card. It is usually
printed on a label attached to the card. If not, see section “Determine your
MAC Address” on page 85.
• ip=<IP address - ask your system administrator>
This is the IP address that will be assigned to the card after the BootP
request. This IP address must be configured using the Agilent ChemStation
Configuration Editor.
• sm=255.255.255.0:\
This is the subnetmask assigned by the BootP request.
• gw=<gateway address - ask your system administrator>
This is the gateway address assigned by the BootP request.
• T145=64:T146=01:T147=01
These parameters configure the buffer handling of the G1369C LAN card.
These are required for the instrument on the LAN to work properly and
must not be changed.
Different entries for the subnet mask and default gateway might be required,
depending on your network setup. These values need to be set by your network
administrator.

90 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 LAN Communication, Installation, Connection and Configuration 6
Using the Agilent ChemStation Configuration Editor to Configure the Spectrophotometer

Using the Agilent ChemStation Configuration Editor to Configure

the Spectrophotometer

What is the Agilent ChemStation Configuration Editor?

The Agilent ChemStation Configuration Editor is a program that allows the
easy configuration of your Agilent UV-Visible ChemStation software. This
includes
• Selecting communication using LAN
• Configuring the analytical hardware connected to the PC.
• Configuring the paths used for data storage.
• Configuring color display of the Agilent ChemStation.
Since the Configuration Editor modifies the internal communication module
database, the Agilent UV-Visible ChemStation must be closed beforehand.

If LAN connection is used, Microsoft TCP/IP protocol must be installed and configured, the
NOTE LAN interface Card must be installed, the spectrophotometer must be connected to the PC,
and a bootp server must be installed and configured before the spectrophotometer(s) can
be used. See “Installing and Configuring TCP/IP on the PC” on page 76 for details.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 91

6 LAN Communication, Installation, Connection and Configuration
Using the Agilent ChemStation Configuration Editor to Configure the Spectrophotometer

Configuring an Agilent Cary 8454 UV-Visible Spectrophotometer

This section describes how to configure an Agilent Cary 8454 UV-Visible
spectrophotometer for LAN communication.
1 Start the Agilent ChemStation Configuration Editor by double-clicking on
the Configuration Editor icon in the UV-Visible ChemStation start-up
group, by choosing Start > All Programs or All Apps > UV-Visible
ChemStations > UV-Vis Configuration Editor.
2 Select the Configure > Instruments menu option.
3 Select the Instrument Type UVVis, enter an Instrument Name for your
configuration (e.g. 8454 UV-Visible System [1]), choose an Initial Screen
Window Size, and click OK.

Figure 33 Instrument configuration dialog.

4 Select the Spectrophotometer 8454 in the Modules tab, select the LAN
option, and click Add to add to the Selected Modules box.
5 Select Identify by IP Address and type in the IP address of the LAN
interface card. (If a DNS server is installed you can also define the host
name.)

92 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 LAN Communication, Installation, Connection and Configuration 6
Using the Agilent ChemStation Configuration Editor to Configure the Spectrophotometer

6 Click OK to exit and to return to the Device Configuration dialog box,

which now should look like this:

Figure 34 Device configuration dialog (LAN interfacing)

7 Click OK to exit the Device Configuration dialog box and save your
configuration changes by selecting File > Save.
8 Exit the Configuration Editor by selecting File > Exit.
9 Reboot the PC and power cycle the instrument.
10 Wait until the front panel light on the instrument turns green. Then start
the Agilent ChemStation to check if the software communicates with the
spectrophotometer. In case the red error message line Power Fail shows up,
refer either to the following troubleshooting section or repeat the
installation procedure.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 93

6 LAN Communication, Installation, Connection and Configuration
Troubleshooting LAN Communication

Troubleshooting LAN Communication

Power failure reported on the Agilent ChemStation

If your Agilent ChemStation can not connect to the spectrophotometer using
LAN communication, please perform the following troubleshooting steps.

Verify that basic communication is possible

Use the ping command, to verify that the IP address is operational.
1 Click the Search or Cortana icon in the Windows 10 taskbar or Windows 7
Start menu. Type “Run”. Select Run.... In the Open: field type cmd.exe and
then click OK.
2 Type ping <IP-number> and press Enter to verify communication of the
LAN Interface Card and the LAN interface in your PC. <IP-number> needs
to be replaced by the appropriate IP address (e.g. 10.10.10.101) or host
name.
3 The command ping will send a request for reply to the IP address, bypassing
part of the Windows TCP/IP settings. A successful ping would look like this:
Reply from 10.10.10.101: bytes=32 time<10ms TTL=128
If the message request timed out is displayed, the IP address can not be
reached by the ping command.
4 If the ping request was answered successfully by the instrument, you need
to verify that your Windows TCP/IP settings are correct for the selected
network, especially the subnet mask and gateway settings should be
checked.

Identify by host name

If you identify the UV-Visible system by host name, ensure that the used host
name and IP address are correctly setup in the DNS server. Try using the IP
address in the Configuration Editor.

94 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 LAN Communication, Installation, Connection and Configuration 6
Troubleshooting LAN Communication

Verify that the LAN Interface card is correctly configured

To ensure that all parameters of the LAN interface card are set correctly, use
the Agilent BootP Service program from the Agilent UV-Visible ChemStation
CD-ROM and disable any other means that configure the LAN interface card of
the instrument. Install the bootp service as described in section “Using the
Agilent BootP Service” on page 82 and configure it for the MAC address of the
LAN interface card.

DHCP server
Make sure that no DHCP server runs in the same subnet as the instrument(s),
as these servers also may respond to a bootp request and do not set the buffer
handling of the LAN interface card correctly, i.e. T145=64:T146=01:T147=01.
They also may assign a different IP address to the instrument each time it is
started.

If a DHCP server is running in the same subnet, make sure that fixed IP addresses are
NOTE reserved for your instruments. Ask you IT consultant to make sure that no IP address
conflict may occur.

Frequent buffer overrun in Agilent Cary 8454 logbook

Please contact your system administrator, to check if the network is not
capable of the network traffic induced due to the data acquisition of the
instrument(s). This can also be caused by an incorrectly configured buffer
handling of the LAN interface card (correct settings:
T145=64:T146=01:T147=01).

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 95

6 LAN Communication, Installation, Connection and Configuration
Troubleshooting LAN Communication

96 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide

A
Safety Information

Agilent products must only be used in the manner described in the Agilent
product user guides. Any other use may result in damage to the product or
personal injury. Agilent is not responsible for any damages caused, in whole
or in part, by improper use of the products, unauthorized alterations,
adjustments or modifications to the products, failure to comply with
procedures in Agilent product user guides, or use of the products in violation
of applicable laws, rules or regulations.

Protective Earth

Shock Hazard
WARNING
Connecting the Agilent Cary 8454 UV-Vis to a power source that is not equipped with
a protective earth contact creates s shock hazard for the operator and can damage
the instrument. Always operate your instrument from a power outlet which has a
ground connection.

Disconnecting Device

Shock Hazard
WARNING
To disconnect the instrument from line power, disconnect the power cord from the
supply. The power supply still uses some power and a shock hazard remains inside
the instrument, even if the power switch on the front panel is turned off.
For safety reason do not position the equipment so that it is difficult to operate the
disconnecting device.

Agilent Technologies 97
A Safety Information

UV Light Source

Eye Hazard
WARNING
Eye damage may result from directly viewing light produced by deuterium lamps.
Always turn off the deuterium lamps before opening the lamp door on the
instrument. Direct viewing of ultraviolet rays without protection causes permanent
blindness or vision impairment.

Equipment Class
The Agilent Cary 8454 UV-Vis instrument is suitable for indoor use only and is
classified suitable under Equipment class I category.

Installation category
The installation category is II, based on IEC61010-1. The installation category
implies the regulation for impulse withstand voltage. It is also called the 'Over
voltage category'. 'II' applies to electrical equipment with a nominal supply
voltage up to 300 V.

Pollution level
The pollution level is 2, based on IEC61010-1. Pollution level describes the
degree to which a solid, liquid or gas that deteriorates dielectric strength is
adhering. '2' applies to a normal indoor atmosphere, where only
non-conductive pollution occurs.

98 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

Index
A connecting power to your front view, 21
spectrophotometer, 24 fuses, 17
accessory boards slot, 24 connecting tubing, 47
accurate results, 32 consumption (line power), 17
acetic acid, 39
G
contents of shipping container, 20
acetone, 39 cooling air, 18 general purpose input/output
acetonitrile, 39 connector, 24
cut-off filter, 41
adding new instruments, 73 glass cells, 33
cut-off wavelength, 41
additional qualification, 30 glycerol, 39
cyclohexane, 39
air circulation, 19 GPIO connector, 24
cyclopentane, 39
air for cooling, 18 ground connection, 18
ambient stray light, 21
D H
analytes, 32
apertured cells or cuvettes, 35 degassed, 40
handling cells, 38
apertures, 41 device configuration, 72
hardware
automated self-test, 30 dimethyl formamide, 39
installing, 60
dimethyl sulfoxide, 39
hardware address, 82, 85
B dirty filters, 56
highest precision measurements, 36
display, 26
baseline noise, 30 homogeneity, 42
dust, 40
benzene, 39 host name, 90, 94
blank, 32, 42 HPLC grade water, 40
E
blank pushbutton, 23
bootp server, 82 electronic self tests, 28 I
bubble formation, 40 entrapment of bubbles, 36
indicator, 28
buffer handling, 90 error condition, 22, 23
indicator on front panel, 22
ethyl acetate, 39
Installation of the TCP/IP protocol, 76
C ethyl ether, 39
installing
excessive noise, 30
carbon disulfide, 39 hardware, 60
carbon tetrachloride, 39 installing the waste tubing, 25
F instrument icons, 30
chloroform, 39
cleaning cells, 37 fan, 28 instrument types in configuration editor, 83
colloidal dispersions, 40 flow cell, 36 isopropyl alcohol, 39
common solvents, 39 flow cells, 44
computer video socket, 26 frequency (line power), 17 J
configuration editor, 72, 92 front panel indicator, 22
JetDirect card
front panel pushbuttons, 23
spectrophotometer IP addresses, 81

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 99

Index

K optical filter, 41 sample cell, 32, 40

optical self tests, 28 sample degradation, 56
keyboard, 26
optical specifications of cells, 34 sample pushbutton, 23
optical surfaces, 37 screen element color, 73
L selftests, 22
lamp intensity, 29 P sensitivity, 35
lamps start turning on, 28 setting up an additional
parallelism, 34 spectrophotometer, 72
LAN, 79
particulate matter, 40 shipping container contents, 20
LAN adapter
passivating new cells, 37 signal to noise ratio, 41
JetDirect card, 79
photochemical reactions, 41 single beam instrument, 32
TakkToLab card, 79
photodegradation, 41 sipper system, 36
Lan Adapter, 79
photographic lens tissues, 56 sipper/sampler system, 40
lens tissues, 37
photosensitive substances, 41 software certificate and registration
license registration number, 67
pipette, 38 packet, 67
light beam, 34, 37
plastic sample cells, 33 solute convection, 40
line frequency, 17
poor linearity, 35 solution, 42
line power consumption, 17
poor photometric accuracy, 35 solution noise, 40
line power input socket, 24
power consumption, 17 solvent, 32
line power supply, 17
power supply, 17 solvent suitability, 39
line power switch, 22
power switch, 22 solvents, 39
line voltage, 17
printer cable, 26 spectrophotometer IP addresses, 81
liquid samples, 32
proper operating condition, 30 spikes, 30
pushbuttons, 23 stabilize, 29
M pyridine, 39 standard cells, 44
MAC address, 82, 85, 90 standard pushbutton, 23
magnetic impeller, 48 Q standard single-cell cell holder, 44
magnetic induction stirring, 48 stirring, 40
quartz sample cells, 33
making measurements, 32 stirring module, 41
methanol, 39 stop pushbutton, 23
methyl formate, 39 R
stoppered sample cell, 40
MIO board slot, 24 rapid absorbance changes, 41 stray light, 21
mouse, 26 ready to measure, 28 sulfuric acid, 39
multicell connector, 24 recommended cells, 35 supply (line power), 17
m-xylene, 39 red blinking error condition, 23, 28 switches
red error condition, 22, 28 power, 24
N remote connector, 24
repeat ignition cycle, 28
n-butyl alcohol, 39 T
RS 232 connector, 24
n-hexane, 39 TalkToLab, 79
S temperature control, 40
O thermal equilibrium, 47
safety light switches, 24 thermostattable cell holder, 41
offline mode, 30
sample, 32, 42 thermostatted water, 47
online mode, 30

100 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

 Index

thermostatted water bath, 46

toluene, 39
trimethylpentane
2,2,4-trimethylpentane, 39
turn on your spectrophotometer, 27
turn-on self-test, 30

U
useful wavelength range of solvents, 39
UV grade water, 40

V
video socket on computer, 26
viscous solutions, 41
volatile solvents, 40
voltage (line power), 17

W
waste container, 25
water, 39
wedge shaped cells, 34

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 101

Index

102 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide

www.agilent.com

In This Book
This handbook describes the
steps needed to install your
new Agilent Cary 8454
UV-Visible spectroscopy
system.
Most of the handbook deals
with installing a complete
system, that is,
spectrophotometer, computer
and printer. However, in the
second half of this handbook
you will find additional
information about installing
mechanical and optical
accessories, accessory boards,
GPIB connections and
configuration, and software
installation.

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2013, 2014-2016

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