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Act 2 Bot Lab

The document discusses the parts and functions of the compound microscope. It provides labeled diagrams of the compound microscope and lists the main parts including the objectives, eyepiece, illuminator, condenser, stage, adjustment knobs, and body tube. It also describes the functions of the low power objective, high power objective, oil immersion objective, and other parts used for illumination, focusing, and holding the microscope components. Different types of microscopes are also enumerated such as stereo, digital, USB, pocket, electron, and scanning probe microscopes along with their basic functions.

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Amy Balicag
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54 views

Act 2 Bot Lab

The document discusses the parts and functions of the compound microscope. It provides labeled diagrams of the compound microscope and lists the main parts including the objectives, eyepiece, illuminator, condenser, stage, adjustment knobs, and body tube. It also describes the functions of the low power objective, high power objective, oil immersion objective, and other parts used for illumination, focusing, and holding the microscope components. Different types of microscopes are also enumerated such as stereo, digital, USB, pocket, electron, and scanning probe microscopes along with their basic functions.

Uploaded by

Amy Balicag
Copyright
© © All Rights Reserved
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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The Compound Microscope

1. Draw and label a compound microscope.


Name: Rating:
Course/year:
Laboratory schedule:

Explain the inverted image observed under the microscope.


It is inverted because of the microscope itself having several lenses. The image
focused by the lens crosses before the eyepiece further magnifies what we see and the
objective lens inverts the image because of the lens’ curvature. When the letter ‘e’ is
put right-side up in the slide, it is projected upside down in the tube. While exploring,
I moved the slide towards the right but it shifts the image towards the left. Also, it is
not only inverted but also backward or reversed.
Exploring the image under the microscope, I moved towards to the upper right
area of the stage, the letter moves towards the lower left. The same goes when I move
it to the lower left of the stage, it moves towards the upper right. Also, adjusting the
coarse and fine adjustment knob made the image clearer. On the 100x, you need to
adjust the light to further lighten the image.

1. Enumerate other different types of microscopes and give their functions.


Stereo Microscope- also called as dissecting microscope, has two optical paths at
slightly different angles allowing the image to be viewed three-dimensionally under
the lenses. It magnifies at low power, typically between 10x and 200x, generally
below 100x. Stereo microscopes also allows observer to observe plant photosynthesis
in action.
Digital microscope- uses the power of the computer to view objects not visible to
the naked eye. It connects to a monitor via USB cable. This allows the monitor to
display the magnified specimen. Moving images can be recorded or single images
captured in the computer’s memory.
USB Computer Microscope- essentially a macro lens used to examine images on
a computer screen plugged into its USB port
Pocket Microscope- used by scientists for hand-held imaging of a variety of
specimens/objects in the field or in the laboratory, ranging from 25x to 100x.
Electron Microscope- a powerful microscope available and used today, allowing
researchers to view a specimen at nanometer size.
A. transmission electron microscope (TEM)- capable of producing images 1
nanometer in size
B. Scanning electron microscope- produce high-resolution, sharp, black and
white 3D images
Scanning Probe Microscope- images are highly magnified and are observed as
three-dimensional-shaped specimens in real time. SPMs employ a delicate probe
to scan the surface of the specimen eliminating the limitations that are found in
electron and light microscopy
Acoustic microscope- used to view a sample internally without staining it or
causing it any damage thanks to point focusing technology, which relies on a
beam to scan and penetrate the specimen while it is in water.

Parts Functions

A. Magnifying Parts
a. Objectives
Low power objective (LPO) It is the shorter objective with 10x (magnifying power of
the lens) and 16mm (focal length) and provides a general
view of the specimen.

High power objective (HPO) It is usually marked 40x to 60x and 4mm and gives a
more detailed view of the specimen.

Oil immersion objective (OIO) It is marked 97x-100x and 1.8mm that gives the highest
magnification among all objectives of the compound
microscope.
Eyepiece/ocular It further magnifies the images formed by the objectives.

B. Illuminating Parts
Illuminator/mirror Provides light that reflects to the specimen

Iris diaphragm Located below the condenser and provided with small
lever which can be moved to regulate the amount of light
that enters the condenser.
Condenser It is found beneath the stage and concentrates the light
into specimens in focus.

C. Mechanical Parts
Base Supports the entire microscope
Arm Supports the upper parts of the microscope and used to
carry the microscope
Body tube Holds the tube and revolving nose piece
Revolving or rotating nose piece Holds the objective lenses in place
Dust shield Concave disc that protects the objectives from dust
Stage Main,flat surface that holds the slides for observation
Stage control Moves the stage left and right or up and down
Light intensity control knob Controls the intensity of light provided by the microscope
D. Focusing Parts
Coarse adjustment knob Brings the specimen into general focus
Fine adjustment knob Tunes the focus and increases the detail of the specimen

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