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Amino Acid Titration. Sci Paper

This document describes an experiment involving the titration of the amino acid lysine. Key results include: 1) The titration curve of lysine allowed for determination of its pKa values, which were close but not identical to theoretical values, likely due to experimental errors. 2) Lysine was shown to act as a buffer in the basic pH range due to its three ionizable groups. 3) The health benefits of lysine include supporting immune function and reducing stress and anxiety.

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Liam Jacque Lapuz
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332 views5 pages

Amino Acid Titration. Sci Paper

This document describes an experiment involving the titration of the amino acid lysine. Key results include: 1) The titration curve of lysine allowed for determination of its pKa values, which were close but not identical to theoretical values, likely due to experimental errors. 2) Lysine was shown to act as a buffer in the basic pH range due to its three ionizable groups. 3) The health benefits of lysine include supporting immune function and reducing stress and anxiety.

Uploaded by

Liam Jacque Lapuz
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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AMINO ACID TITRATION

Liam Jacque M. Lapuz

De La Salle University- Dasmariñas

Dasmariñas, Cavite, Philippines

Abstract:

Titration is a chemical analysis method used in laboratories to determine an unknown


concentration of an identified increment. It can also be used in identifying unknown
amino acids and Titration curves can also be used to determine the value. In the plotted
data of Lysine, it shows that errors are present in the experiment in the values and
percentages in the experiment. Fortunately, the data acquired from the experiment
shows that Lysine can act as a buffer in a basic range in pH and that the health benefits
of Lysine is also evident.

Introduction:

Titration or Titrimetry, is a common quantitative chemical analysis method used in


laboratories to determine an unknown concentration of an identified increment (1). The
method of titration requires specific instruments, techniques, and reagents in order to
perform the given method. (1) In addition, it is a method of slowly adding one solution with
a known concentration to a known volume of another solution with an unknown
concentration until the reaction reaches the desired level (2). However, when the process
of titration is done, the pH values of the analyte solution must be plotted through the use
of titration curves (3). In the case of amino acids and peptides, the plotted values
represent strength of different prototrophic groups, their buffering zones, and their
respective isoelectric pH values (3).

Materials and methods:

For this experiment, a .250 g of Lysine is placed in a 250 ml beaker and weighed using a
top loading balance. Next, 15 ml of distilled water is added to the beaker and it is stirred
to dissolve the sample. With a use of a syringe, add 200 M of HCI to the mixture and stir
until the pH solution drops to 1.50. Moving on, a Titration of .20 ml increment of .200 M
NaOH. Afterwards, the titration was stirred and measured until the pH level of the solution
reaches past 11. Following the measurements, a graph was created to plot the values.
Then, estimate the pKa values from the inflection points from the curve. The determined
pKa values are compared to the theoretical values and calculate the percent errors for
the pKa values. Finally, the titrimetric profile of lysine is drawn and the theoretical and
experimental pI of Lysine and the percent error is determined.

Results & Discussions:

Figure 1. Titration Curve of Lysine

In order to determine an unknown amino acid, the data that resulted from the Titration of the experiment is
used. As shown in figure 1, the pKa values are carefully plotted in the graph that can be used in determining
the amino acid. With all the gathered values, the results show that the unknown amino acid is Lysine.

Experimental Theoretical % Error

pKa 1 2.1 2.18 3.67%

pKa 2 9.0 8.95 0.56%

pKa 3 10.1 10.79 6.39%


pI 9.85 9.87 3.24%

Table 1. pKa Values and Identity of Lysine

Unfortunately, there are some errors between the Theoretical values. Specifically, the
percentage errors. The possible reasons for these errors are due to common human
errors and machine errors. When titrating any chemical solution, it requires careful
preparation in order to get the desired results. Unfortunately, every individual has the
tendency to make an error during the process. A common mistake is weighing the solution
incorrectly or inaccurate measurement of liquids. With this in mind, it is expected that
results from every titration may vary due to the common mistakes that individuals make.
Next, an error in the pH meter may result in obtaining inaccurate pH values. Finally,
temperature is needed in order to get accurate results from the experiment. A
measurement of 298k must be maintained all throughout the process.
Any fluctuation in the temperature may result in inaccurate results.

Figure 2. Titrimetric profile of Lysine

The structure of Lysine shown in figure 2 shows that Lysine can act as a better buffer
when it is at high pH values due to its three ionizable groups (two amino groups and one
carboxyl group). Hence, if the amino acid is placed in a highly acidic solution, it will be
fully protonated and will have a charge of +2. Then, the addition of base slowly
deprotonates the amino acid and can act as buffers at a particular pH. Therefore, lysine
can act as a buffer in the basic pH range and the pKa and pI level of Lysine can also be
observed.
L-lysine helps your immune system by promoting healthy immune cell activation. This
amino acid promotes the action of IgG antibodies, which aid in the detection and
protection of environmental and seasonal dangers. L-lysine also helps to boost
immunological responses and reduces oxidative damage. (4)

The immune system might be harmed by modern lifestyle choices that do not include
balanced foods and daily stress. L-lysine helps immune cells function properly, resulting
in a strong, healthy immunological response and protection against environmental
dangers (5). Lysine may help to alleviate anxiety. It was discovered in one investigation
that it inhibited stress receptors. Stress-induced loose bowel motions were reduced in
rats fed lysine, according to the researchers .Supplementing with 2.64 grams of lysine
and arginine for a week reduced stress-induced anxiety and cortisol levels in 50 healthy
persons, according to a one-week study.(5)

References:

1. Titration - an overview | ScienceDirect Topics. (n.d.). Science Direct.Com.

Retrieved October 28, 2021, from

https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/titration

2. A. (2021, March 22). General Data Protection Regulation(GDPR) Guidelines

BYJU’S. BYJUS. Retrieved October 28, 2021, from https://byjus.com/jee/titration/

3. What Is Titration? See Titration Curves. (2019, June 26). ThoughtCo. Retrieved

October 28, 2021, from https://www.thoughtco.com/acids-and-bases-titration-

curves-603656

4. L-Lysine: Benefits, the Right Dosage, and Warnings. (2015, February 5). 1MD.

Retrieved October 28, 2021, from https://1md.org/health-

guide/immunity/ingredients/immune-l-lysine#:%7E:text=L-

lysine%20is%20a%20form%20of%20lysine%20that%20your,of%20fat%20cells%

20to%20be%20burned%20for%20energy.
5. O’Brien Ms, S. P. (2018b, October 6). 4 Impressive Health Benefits of Lysine.

Healthline. Retrieved October 28, 2021, from

https://www.healthline.com/nutrition/lysine-benefits#TOC_TITLE_HDR_4

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