Scribd
Upload
154 views4 pages

Biochem Lab Quipper

The document discusses several biology experiments and concepts. It provides details about enzyme activity in potatoes and saliva, discussing how chopping potatoes and temperature affect catalase and amylase. It also covers nucleic acid structure and tests to identify DNA, RNA, and their components. Additional concepts covered include urinalysis tests to detect substances like glucose, proteins, ketone bodies and more. Blood tests like the Kastle-Meyer test to identify blood and methods to measure total protein and other analytes are also summarized.

Uploaded by

JULIANNE BAYHON
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
154 views4 pages

Biochem Lab Quipper

The document discusses several biology experiments and concepts. It provides details about enzyme activity in potatoes and saliva, discussing how chopping potatoes and temperature affect catalase and amylase. It also covers nucleic acid structure and tests to identify DNA, RNA, and their components. Additional concepts covered include urinalysis tests to detect substances like glucose, proteins, ketone bodies and more. Blood tests like the Kastle-Meyer test to identify blood and methods to measure total protein and other analytes are also summarized.

Uploaded by

JULIANNE BAYHON
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 4

QUIZ 1

 enzyme present in potato: catalase


 highest enzyme-substrate activity: chunks of potato + hydrogen peroxide
 lowest enzyme-substrate activity: piece of cut potato in hot water + hydrogen peroxide
 Enzyme Potato Experiment, what is the substrate: hydrogen peroxide
 By chopping up the potato, we: increase the surface area
 test tube 4 of the Enzyme Potato Experiment, enzyme is denatured chemically using:
acetic acid
 test tube 2 of the Enzyme Potato Experiment, enzyme is denatured due to: heat
 enzyme present in saliva: amylase
 5 degrees Celsius, it takes less time to digest starch by the enzyme salivary amylase:
false
 50 degrees Celsius, it takes more time to digest starch by the enzyme salivary amylase:
true
 It takes less time to reach the achromic point at: 37 degrees Celsius
 5 degrees Celsius, the enzyme, salivary amylase is: deactivated
 50 degrees Celsius, the enzyme, salivary amylase is: denatured
 salivary amylase digested the starch at what pH: 6.8
 used to track the digestion of starch by salivary amylase: iodine solution
QUIZ 2

 a double stranded chain of nucleotides: deoxyribonucleic acid


 single stranded chain of nucleotides: ribonucleic acid
 nucleic acid active in cell signaling: ribonucleic acid
 genetic material used in functioning and development of living organisms:
deoxyribonucleic acid
 extraction of nucleic acid is done by breaking outer cell membrane of any plant tissue
along with cytoplasm: cell lysis
 process of DNA extraction which involved centrifugation: precipitation
 DNA extraction, which comes first in the extraction process: removal of membrane
lipids
 reagent is added to improve the precipitation of DNA: sodium acetate
 basis of analysis of nucleic acid by UV spectrophotometry: amount of light at specific
wavelength can be absorbed in a specific pattern
 test for purine, purine bases are precipitated by what reagent: silver nitrate
 Diphenylamine test will give what positive result: blue complex
 most sensitive test for pentose: orcinol test
 test for reducing sugar: benedict’s test
 color is positive result to the Orcinol Test: greenish-blue
 Bials’ Test, the condensation product is soluble in what particular reagent: amyl alcohol
 test is commonly used for the estimation of RNA: orcinol test
 test for the presence of DNA: diphenylamine test
 reagents were used in the test for purine, except: hydrochloric acid
 tests are test for pentose, except: benedict’s test
 Diphenylamine test is a test for: deoxyribose
QUIZ 3

 included in the chemical examination of urine: glucose


 included in the physical examination of urine except: pH
 urine sample is best for routine urinalysis and microbial culture: clean catch urine
sample
 Pale to colorless urine may indicate the following except: dehydration
 Dark yellow urine indicates: dehydration
 tests are used for the detection of sugar in urine except: heller’s test
 test for albumin wherein a cloudy turbid solution or precipitate is formed:
sulphosalicylic acid test
 test wherein the presence of a green ring at the junction of two layers indicates the
presence of Bile Salts in the urine sample: smith’s test
 reagents are used in the Urease Test except: sulfuric acid
 Rothera’s Test, the purple colored ring at the junction of two solutions indicates the
presence of in urine sample: ketone bodies
 reagent used in the chemical test for Chloride ions: silver nitrate
 reagent used in the chemical test for Sulfates: barium chloride
 Heller’s Test in urine, what reagent denatures albumin: nitric acid
 tests are used for the detection of urea in urine except: pettenkofer’s test
 tests are used for the detection of bile salts in urine except: urease test
 protein produced by the liver and is mainly found in the blood: albumin
 kidney disturbance and high blood pressure, albumin level in the urine is significantly:
high
 yellow green fluid that contains water and organic molecules such as cholesterol, bile
acids, and bilirubin: bile
 humans and most vertebrates, bile is produced by the: liver
 Sugar is ordinarily in normal urine: not present

QUIZ 4

 excellent presumptive test for blood: kastle-meyer test


 oxidizing agent used in the Kastle-Meyer Test: hydrogen peroxide
 reagents are used in the Kastle-Meyer Test except: hydrochloric acid
 added to phenolphthalein reagent to make it colorless (reduced form) in the Kastle-
Meyer Test: zinc
 Kastle-Meyer test for blood, what other material can give a false positive result: potato
 absent in blood serum: fibrinogen
 plasma protein maintains the colloidal osmotic pressure: albumim
 normal concentration of total protein in blood: 6.5-8.5 g/ dL
 Biuret Method for the estimation of total protein in blood, what is the reagent in the
test tube labeled blank: normal saline
 Using the colorimeter, what is being measured to compute for the total protein in blood
in the Biuret Method: optical density
 volume of reagent used in the Creatinine Test using the Anamol Test Kit: 1000μl
 important materials needed to perform the different tests using the Anamol Test Kit
except: dropper
 Anamol Test Kit for Creatinine, Glucose, Calcium, and Cholesterol Tests, how can the
sample be introduced into the instrument: aspirate
 incubation period for the Calcium Test using the Anamol Test Kit: 5 minutes
 Glucose Test using the Anamol Test Kit, the assay mixture is incubated for 10 min at: 37
degrees Celsius

You might also like