Figure . Collected Lab Data for the 1st trial.

Figure . Collected Lab Data for the 2nd trial.

The overall data that was gathered from the experiment are from two trials. Multiple trials were
done to ensure that the results are consistent and to also ensure that the conditions in which the
experiment trials were held are to be considered. The concentrations of the chemicals, as well
as its volumes and colors, are all gathered through observations of the physical attributes and
values from the experiment. The concentration of iron (III) thiocyanate ion in stock solution is
calculated through a series of equations shown down below.

Fe3+ + SCN-  [Fe(SCN)2+]

mol Fe3+ = (molar concentration)(Volume in L)

mol Fe3+ = (0.2)(0.01L)

=0.002 moles

mol SCN- = (0.002)(0.002L)

=0.000004 moles (limiting variable)

[Fe(SCN)2+] (M) = (limiting variable) / (total volume in L)

= 0.000004/0.02

= 0.0002 M

Figure . The numerical data gathered from the diluted solutions of 1st trial.

Figure . The numerical data gathered from the diluted solutions of 2nd trial.

The set of solutions for both trials:

For [Fe(SCN)2+] = (0.0002M/1000mL)x(4mL)

= 8 x 10-7M

Formula: [Fe(SCN)2+] / (total vol (L) of stock solution and DI water)

Solution 1: (8 x 10-7M) / [(4+1)/1000] = 0.000160 M

Solution 2: (8 x 10-7M) / [(4+2)/1000] = 0.000133 M

Solution 3: (8 x 10-7M) / [(4+3)/1000] = 0.000114 M

 Figure . Calibration curve data gathered from the 1st trial.

Figure . Graph of the calibration curve data from the 1st trial.

Regression analysis was used to estimate the impact of some explanatory variable on the
dependent variable or to predict the value of the dependent variable for individuals for whom
some knowledge about the explanatory variables is accessible (Kellogg, n.d.). In the
experiment’s case, the variables are the absorbance and the concentrations of each solution
and as well as the stock solution that prepares the range of working solutions through dilution
(Simulab, 2017).
 Figure . Calibration curve data gathered from the 2nd trial.

Figure . Graph of the calibration curve data from the 2nd trial.

The factor that leads to the differences in values of both trials is the wavelength value that is set
in the spectrophotometer and the one who assigns the different values is the laboratory
simulation itself. The first trial had 446 nm as its assigned value while the second trial had 448
nm. The wavelength value affects the absorbance that the spectrophotometer gathers from
each of the cuvette containing the sample solutions, thus the difference in values of both trials.
Through the process of regression analysis, the linear regression equation was provided as well
as the values of the molar absorptivity. The graphs from both trials also showed that the
absorbance and concentration are directly proportional.

Table 1. Molar absorptivity of Trial 1 and Trial 2.

Trial Molar absorptivity (M-1cm-1)

1 1359

2 1267

Figure . Table of values for the equilibrium constant Kc for Trial 1.

 Figure . Table of values for the equilibrium constant Kc for Trial 2.

Using a volumetric pipet, the designated volumes of the chemicals and distilled water was put
into the five test tubes that hold each of the sample solutions. Because of the difference with the
assigned wavelength value, the data in the tables also differ from each other but the process or
formulas that were used to solve for them are all the same. The formula below shows how the
initial concentrations of iron (III) nitrate and thiocyanate were solved.

Vtotal = [VFe(NO3)3] + [VKSCN] + [VH2O]

[Fe3+]initial = {[Fe(NO3)3]( VFe(NO3)3)} / Vtotal

[SCN-]initial = {[KSCN]( VKSCN)} / Vtotal

If the aforementioned formulas were performed the values in the rows Initial concentration
[Fe3+] (M) and Initial concentration [SCN-] (M) will then be gathered. The absorbance values
were gathered through the use of the spectrophotometer (different wavelength for each trial).
For the equilibrium concentrations of [Fe3+] and [SCN-], the formula can be seen down below,
as well as the table of values.
[TRIAL 1] For the equilibrium concentration of [Fe3+] and [SCN-]:

Formulas:

 Initial concentration [Fe3+] (M) – Equilibrium [FeSCN2+] (M)

 Initial concentration [SCN-] (M) – Equilibrium [FeSCN2+] (M)

Table 1. Table of equilibrium concentrations for Trial 1.

[Fe3+] (M) [SCN-] (M)

(1) 0.000802 (1) 0.000802

(2) 0.000859 (2) 0.000659

(3) 0.000887 (3) 0.000487

(4) 0.0009235 (4) 0.0003235

(5) 0.000962 (5) 0.000162

[TRIAL 2] For the equilibrium concentration of [Fe3+] and [SCN-]:

Formulas:

 Initial concentration [Fe3+] (M) – Equilibrium [FeSCN2+] (M)

 Initial concentration [SCN-] (M) – Equilibrium [FeSCN2+] (M)

Table 1. Table of equilibrium concentrations for Trial 2.

[Fe3+] (M) [SCN-] (M)

(1) 0.000788 (1) 0.000788

(2) 0.000848 (2) 0.000648

(3) 0.000878 (3) 0.000478

(4) 0.0009179 (4) 0.0003179

(5) 0.000959 (5) 0.000159

To solve for the complex ion concentrations using absorbance, Equilibrium [FeSCN2+] (M), the
formula below will be used.

A = Єℓc
A = Absorbance

Є = Molar absorptivity

ℓ = Cell path length

c = Concentration

In order to get the concentration, the formula will be rearranged to derive the equation for the
concentration.

c = A/Єℓ
Trial 1: c = A / [(1359 M-1cm-1)(1cm)]

Trial 2: c = A / [(1267 M-1cm-1)(1cm)]

If the symbols are to be substituted with its corresponding values then we will get the
Equilibrium [FeSCN2+] (M) values, as can be seen on Figures and . As all the values have
been collected, the Equilibrium Constant Kc can now be solved through the formula shown
below.

Kc = [FeSCN2+] / ([Fe3+][SCN-])
The equilibrium constant values of each sample solution can also be seen on Figures and , for
both trials. The average equilibrium constant can be solved by just adding all the equilibrium
constant values and divide the sum by five, because the total number of sample solutions is
five.

References

Kellog (n.d.). Why do a regression analysis? Interpreting a Regression Analysis.

https://www.kellogg.northwestern.edu/faculty/weber/jhu/statistics/regression.htm#:~:text
=Typically%2C%20a%20regression%20analysis%20is,variable%20on%20the
%20dependent%20variable.
Simulab. (2017). Stock solutions. Simulab Corporation. https://www.simulab.com/about-
simulab#:~:text=Since%201994%2C%20Simulab%20has%20been,to%20the
%20medical%20education%20community.