C101-E168A

UV-VIS Spectrophotometer

UV-1900i
Navigate Your Way

Easy to Operate, Obtain Answers Easily and Rapidly

Easy-to-use user interface design
Ergonomic touch-screen display

High Performance to Meet Diverse Needs

The instrument is equipped with an ultra high-speed scan, which can acquire a
spectrum in a few seconds, with the lowest level of stray light and noise in its class

Advanced Regulatory Compliance

Validation functions enable checks in accordance with Pharmacopeia (JP, USP, and EP)
to be performed easily
In combination with LabSolutions ™
DB/CS, comply with FDA 21 CFR Part 11 and
PIC/S GMP guidelines

UV-i Selection

™
UV-1900i UV-2600i/2700i UV-3600i Plus SolidSpec -3700 i
™
 Easy to Operate,
Ergonomic Obtain Answers Easily and Rapidly
Hardware Design
The control panel is ergonomically designed and positioned
at the optimum viewing angle for the user. Users can
operate easily with any posture. Since the panel is
pressure-sensitive, the device can be operated with gloved
fingers or a stylus pen. The stylus pen can be stored right
next to the control panel.

The instrument is equipped with a

stylus pen, allowing operation by
this pen or a finger.

Easy-to-Use Interface
Grasp the Current Status and Operating Procedures at a Glance
The UV-1900i on-screen user interface includes large, easy-to-see icons deployed on a black background, so the instrument settings are evident at a
glance. In addition, the large, easy-to-see icons improve intuitive understanding, which enables users to quickly become familiar with the operations.
Furthermore, the user interface is designed to minimize transitions between windows, so users do not get confused
Display languages are available in eight languages (Japanese, English, during the
operations.
Chinese, Spanish, Portuguese, German, French, Russian).

Navigation Tabs Improve

Usability
In quantitation mode on the UV-1900i, the stages
of the entire measurement process and the
current status are always shown on the display.
As a result, users know immediately what to do
in the next step.

4
High Performance to Meet Diverse Needs 3.00
Abs. Calibration Curve

Instrum ent with 1.00 % Stray Light

2.50 UV-1900i
Ultra-Fast Scan 2.00

Spectra can be acquired as fast as 29,000 nm/min. Ultra-fast scan is effective in tracking chemical reactions in a short time. In
1.50
addition to the absorbance change at specified wavelengths, spectra can also be acquired in a short time with the UV-1900i.
1.00
Therefore, more detailed behavior can be investigated by observing spectra with the UV-1900i.
0.50
The figures below show the analysis of the particle agglomeration process when salts are added to silver nanoparticles.
0.00
Measurements of the 300 to 700 nm region were performed in ultra-fast scan mode. In addition to the decrease of
0.00 1.00 2.00 3.00
absorbance at 400 nm and the increase of absorbance at 480 nm, the temporal changes of spectra can also be observed. Conc. (mg/L)

1.0 0.23
0.97
Black: after 0 min
Red: after 0.5 min 0.21
Blue: after 1 min 0.92 480nm
0.8
Green: after 2 min
0.19
Orange: after 5 min

Absorbance at 400 nm (Abs)

Absorbance at 480 nm (Abs)

Purple: after 10 min 0.87
0.6 0.17
Abs.

0.77 0.15

0.4
0.13
0.72
400nm 0.11
0.2
0.67
0.09

0.0 0.62 0.07

300.0 400.0 500.0 600.0 700.0 0 100 200 300 400 500 600
nm Time (sec)
Absorption Spectra of Silver Nanoparticles Temporal Changes of Absorbance at 400 and 480 nm

Low Stray Light

Stray light is at 0.5 % max. (198 nm), making accurate measurements are possible up to the vicinity of 2 Abs even in the ultraviolet region. In addition,
high-concentration samples can be quantified accurately.
The figure on the right is a calibration curve for acetic acid, created with absorbance at 200 nm.
The correlation coefficient is 0.9997 and correct measured values are obtained even in the vicinity of 2 Abs. Linearity will be lost in the high
absorbance region due to the stray light.

High Reproducibility and Repeatability Accuracy

The photometric repeatability accuracy is 0.0002 Abs max. (0.5 Abs and 1.0 Abs). With this high photometric repeatability accuracy, variance in the
measurement results is suppressed, enabling more accurate quantitation and the detection of low-concentration samples.
Abs. Calibration Curve

The figure on the right is a calibration curve for caffeine, created with absorbance at 273 nm. The
calibration curve has an Abs = 0.0528 Conc. The lower limit of quantitation determined from the
standard deviation is 0.0051 mg/L.

UV-1900i
UV-VIS Spectrophotometer 5
2.5

2.0
 No. Absorbance of Blank Solution (273
nm) 1.0
1 -0.00001
2 0.00001 0.5
3 -0.00002
4 0.00002
0.0
Note: One method of determining the lower limit of quantitation is to use ten 5 0.00001 0.0 10.0 20.0 30.0 40.0
times the standard deviation. 6 -0.00003
This is an actual measured value and is not guaranteed.Conc. (mg/mL) 7 0.00001
8 -0.00004
Various Functions for 9
10
0.00001
0.00005
Comfortable Daily
Standard 0.000025
Measurement Deviation σ

Network Connectivity Function

The UV-1900i provides network access via wireless connectivity.

Data can now be transferred to a PC via a network.
Thanks to wireless printing, multiple UV units can print from a single printer.
(A router and other network equipment must be installed to use a network.)

1 2
Data transfer function* PictBridge function*

*1: Optional expanded memory is required. The instrument is not compatible with control via a network.
*2: A PictBridge compatible printer is required.

Sleep Mode and Wakeup Function

Analysis can start the instant the user arrives at the laboratory in the morning. The instrument requires no time to warm up.

Previous Next
day Start Time morning
Ready
9 : 00

Bar-Code Reader and Keyboard Entry Function

Sample names and numerical values can be entered by a bar-code reader or from the keyboard.
This saves time when entering sample names for a multiple sample analysis, and prevents sample misidentification and other human errors.* 3

6
 *3: Use a bar-code reader and keyboard with a USB connection.

A Diversity of Measurement Modes

Photometric Spectrum Quantitation

Measures the photometric value at a Measures a sample spectrum using Generates a calibration curve from the single wavelength or
multiple (up to wavelength scanning.measurement of standards, and then
eight) wavelengths. calculates the concentrations of

UV-1900i
UV-VIS Spectrophotometer 7
 unknowns.

Kinetics Time Course Biomethod

Measures absorbance changes as a function of Measures changes over time in Quantifies DNA or protein
time, and obtains the enzymatic activity values. photometric values at a specified concentrations.
The kinetics measurement method or the rate wavelength.
measurement method can be selected.

Applications Pharmaceuticals and Life Sciences

Foods
This is an example of the analysis of food dyes. By using ultra-fast scan
mode, the time needed for routine spectral checks can be shortened. The
350 nm to 900 nm region can be measured at 1 nm intervals in approx. 4
seconds.

8
This is an example of the analysis of λDNA. Trace quantities (on
1.00

0.80

Absorbance (Abs.)
0.60

0.40

0.20

0.00
350 400 600 800 900
Wavelength (nm)

Absorption Spectra of Food Dyes

Chemistry
This is an example of the analysis of colored cellophane tape. Materials can be
confirmed quantitatively by using LabSolutions UV-Vis and color measurement
software.
100 0.90

0.80

80 0.70

0.60
Transmittance (%)

60 0.50
y

0.40

40 0.30

0.20

20 0.10

0.00
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80
0 x

380 500 600 700 780

Wavelength (nm)
Transmittance Spectra of Colored Cellophane Tape Chromaticity Diagram of XY Color System

Accessories

UV-1900i
UV-VIS Spectrophotometer 9
 Expanded Memory (P/N 207-23119-41) Test Tube Holder (P/N 207-23510-41)

The UV-1900i main unit can store up to 999 sets of data. Holds test tube instead of
The saved data can be read out from a PC. sample compartment.
(A network connection is required for data readout.) Specifications:
Outside diameter: φ15 to 22 mm
Film Holder ( P/N 204-58909) Height: 75 to 115 mm
Note: Test tube is not included.
Used in transmittance
measurement of thin samples
such as films and filters. It Multi-Cell Sample Compartment (P/N 206-69160-41)
holds the samples for
analysis. Holds up to six 10-mm square
cells on the sample side. No
temperature control capability.
Long-Path Rectangular Cell Holder (P/N 204-23118-01) Number of cells: 6 on the sample side

Holds two rectangular cells with an optical 1 on the reference side path length of 10, 20, 30, 50, 70, or 100 mm. Note: Square
cells are not included, please purchase separately. Sipper Unit

Model P/N Standard Sample

Volume
Sipper Unit 160L (Standard Sipper) 206-23790-51 2.0 mL
Sipper Unit 160T (Triple-Pass Sipper) 206-23790-52 1.5 mL
Sipper Unit 160C (Constant-Temperature Sipper) 206-23790-53 2.5 mL
Sipper Unit 160U (Supermicro Sipper) 206-23790-54 0.5 mL
Four types of sipper units with different flow cells are available. The A USB adapter CPS (P/N 206-25234-91) is required.
stepping motor-driven peristaltic pump ensures reliable and smooth Note: The use of a Solenoid Valve (Fluoropolymer) (P/N 204-06599-01)
aspiration of sample solution. and the SWA-2 Sample Waste Unit (206-23820-58) are
(Direct driving is possible from the UV-1900i so no interface is required.) recommended when strong acids, strong alkalis, or organic solvents
are to be measured.

CPS-100 Cell Positioner, Thermoelectrically Temperature

Controlled (P/N 206-29500-41)

TCC-100 Thermoelectrically Temperature Controlled Cell

This attachment permits measurement of up to six sample cells under Holder (P/N 206-29510-41)
constant-temperature conditions. Combination of this attachment and
the Kinetics mode provides measurement of temperature-sensitive
enzyme kinetics of one to six samples. Uses Peltier effect for controlling the sample and reference temperature,
so no thermostated bath or cooling water is required.
Number of cells: 6 on the sample side (temperature-controlled)
1 on the reference side (temperature not controlled) Number of cells: One each on the sample and reference sides
Temperature control range: 16°C to 60°C (temperature-controlled)
Temperature display accuracy (difference from the true value): ± Temperature control range: 7°C to 60°C
0.5°C Temperature display accuracy (difference from the true value):
Temperature control precision (variation of temperature): ± 0.1°C ± 0.5°C
Ambient temperature: 15°C to 35°C Temperature control precision (variation of temperature): ± 0.1°C
Note: Square cells (P/N 200-34442) are not included, please purchase
Note: Square cells (P/N 200-34442)
separately.
are not included, please
purchase separately.

10
Standard Software: LabSolutions UV-Vis

The combination of the UV-1900i, offering lower stray light levels and higher accuracy,
with LabSolutions UV-Vis software, which achieves labor-saving measurements,
provides a more convenient analytical environment.

Setting Parameters
From Measurement to Data Output

Smooth Operability Improved Productivity of Data Analysis Operations

Four Measurement Modes Data analysis and data output operations can be performed at

the same time (simultaneously) as data measurement. Time

Four separate measurement modes for spectral, quantitative, photometric, time-course,

and automatic measurements spent outputting or analyzing data can also be reduced by (optional) enable measurements to be performed using

intuitive simultaneously sending data to an Excel® spreadsheet in real

operations. time or saving data as text. The software can also automatically

UV-1900i
UV-VIS Spectrophotometer 11
 perform post-processing of measured data, such as

of measurement results (automatic spectral evaluation).

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Automatic Spectral Evaluation Stronger Data Management

Data Management

(Spectral Evaluation Function)

In addition to regular file management in folders on a PC, ideal
By specifying various evaluation criteria for measurement solutions for saving data in a database with sophisticated
results, spectra judgments can be made automatically. security functionality and compliance with ER/ES-related
regulations are also available.

Optional Software
LabSolutions DB UV-Vis
LabSolutions CS UV-Vis

Database Management
Managing data in a database can prevent the overwriting or
deletion of analysis data. Furthermore, during postrun
analysis, the data can be managed using version numbers,
so there are no concerns about overwriting the data.

Postrun Postrun
Analysis analysis analysis
Ver. 1 Ver. 2 Ver. 3

In the report creation window, reports can either be prepared based Reduces operator errors.
on a previously specified report format or freely laid out based on
various parameters, data, or other elements.

UV-1900i
UV-VIS Spectrophotometer 13
 Advanced Regulatory Compliance

Full Support for Pharmacopeia, GLP/GMP, FDA 21 CFR Part 11 and Other Regulations
Instrument Validation Functions Compliant with JP, USP, and EP
This instrument can not only run checks for nine JIS items, but also those stipulated in the Japanese Pharmacopoeia (JP), United States Pharmacopeia
(USP), and the European Pharmacopoeia (EP). Naturally, the hardware is also compliant with the specifications required by each Pharmacopeia. In
addition, the check conditions can be saved. As a result, once the conditions are saved, checks can be performed easily just by calling them up as
needed. Check results can also be saved.

Main Screen

Parameters Setting Screen

Check Results Screen

During Testing Screen

Improved Security Functions

An external control security function has been added to provide more support for compliance with
regulations. Three user authority levels, "Administrator", "Developer", and "Operator", can be set for
instrument users.

Resolution of 1 nm, the Highest in its Class

In addition to achieving a resolution of 1 nm, the highest in its class, by using a monochromator with a Czerny-Turner mounting, the UV-1900i also
features a compact, bright optical system. The instrument is more than capable of meeting the wavelength resolution required in the European
Pharmacopoeia.

14
Compatible with Validation from PC Software

Validation can be implemented with PC software by using the optionally available UV validation software.
In addition to simplifying daily inspections, this makes instrument performance checks and records management easier, enabling more secure
regulatory compliance.

• Inspection results can not only be printed but also saved to a file, so the • The user can select confirmation of instrument performance indicators results can be called up
later for confirmation. as per JIS K0115 General rules for molecular absorptiometric analysis, as
• The inspection parameters can also be saved to separate files for well as the general test methods in the Japanese Pharmacopeia, USP periodic and routine
inspections, and then called up for use. and various EP inspections. (Order inspection jigs and reagents separately.)

Support for FDA 21 CFR Part 11, PIC/S GMP Guidelines and Other Regulations and Guidelines
Ensuring the integrity of data (database management), including the user management, user authority management, and data audit trails required for
compliance with FDA 21 CFR Part 11, PIC/S GMP guidelines, and other ER/ES regulations, is possible.

LabSolutions DB UV-Vis or UVProbe / LabSolutions DB System

The system allows for data management and user management with a database.
Compliant with ER/ES regulations, the system is optimally configured for customers
using a PC.

LabSolutions CS UV-Vis or UVProbe / LabSolutions CS System (Network

System)
The system is optimally configured for customers who want to manage data on a server together with LC and GC data for ER/ES

UV-1900i
UV-VIS Spectrophotometer 15
 UV-1900i
UV-1900i Website
https://www.shimadzu.com/an/molecular_spectro/uv/uv-1900/index.html

-Automated support functions utilizing digital technology, such as M2M, IoT , and Artificial Intelligence
(AI), that enable higher productivity and maximum reliability.
-Allows a system to monitor and diagnose itself, handle any issues during data acquisition without user
input, and automatically behave as if it were operated by an expert. -Supports the acquisition of high
quality, reproducible data regardless of an operator’s skill
level for both routine and
demanding applications.

www.shimadzu.com/an/
LabSolutions and Analytical Intelligence Logo are trademarks of Shimadzu
Corporation.
TrayCell is a registered trademark of Hellma GmbH & Co. KG. in Germany.
Excel is a registered trademark or trademark of the Microsoft Corporation in the
USA and other countries.
For Research Use Only. Not for use in
diagnostic procedures.
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references to products that are not
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The contents of this publication are

provided to you “as is” without
warranty of any kind, and are subject
to change without notice. Shimadzu
does not assume any responsibility or
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direct or indirect, relating to the use
of this publication.

© Shimadzu Corporation, 2019

Edition: November 2019, Printed