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Activity 4 Organogenesis in The Frog Embryo Activity Worksheet A. 4 MM Frog Embryo

The document describes the identification and labeling of structures in serial sections of 4 mm and 10 mm frog embryos. For the 4 mm embryo, students are asked to examine pictographs, identify structures, label selected sections, and describe the fate of structures. For the 10 mm embryo, students similarly identify structures, label sections, and describe the origin of structures from germ layers. The goal is for students to learn organogenesis by analyzing embryonic development stages.

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122 views

Activity 4 Organogenesis in The Frog Embryo Activity Worksheet A. 4 MM Frog Embryo

The document describes the identification and labeling of structures in serial sections of 4 mm and 10 mm frog embryos. For the 4 mm embryo, students are asked to examine pictographs, identify structures, label selected sections, and describe the fate of structures. For the 10 mm embryo, students similarly identify structures, label sections, and describe the origin of structures from germ layers. The goal is for students to learn organogenesis by analyzing embryonic development stages.

Uploaded by

jhayyypee jhayyps
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Activity 4

ORGANOGENESIS IN THE FROG EMBRYO

Activity Worksheet

A. 4 mm Frog Embryo

1. Examine pictographs of serial sections of the 4 mm frog embryo. Start with the most anterior section
and proceed up to the most posterior section. It would be best to follow organs and organ systems one
at a time.

2. As the serial sections are examined, take note of and identify the following structures (if present, since
some of the structures may or may not really be observable depending on how the embryo was
prepared and sectioned). The list below may serve as a checklist.

• Prosencephalon • Oral plate • Visceral cleft/groove


• Prosocoel • Stomodeum • Visceral pouch
• Telencephalon • Pharynx • Mandibular arch
• Telocoel • Liver diverticulum • Hyoid arch
• Diencephalon • Thyroid rudiment • Hyomandibular pouch
• Diocoel • Proctodeum • Hyomandibular cleft
• Mesencephalon • Yolky endoderm • Notochord
• Mesocoel • External gills • Subnotochordal rod
Infundibulum Aorta (dorsal, Epimere
• • •
Rhombencephalon ventral) Hypomere (lateral plate)
• • •
Rhombocoel Aortic arch Dermatome
• Spinal cord • Vitelline veins • Myotome
• Epiphysis • Heart • Sclerotome
• Retina • Pericardial coelom • Adhesive gland head
• Hypophysis • Pericardium • mesenchyme
• Optic stalk • Conus arteriosus • Epidermis
• Optic vesicle • Myocardium • Coelom
• Optic cup • Endocardium •
• Opticoel • Pronephros
• Lens placode • Pronephric tubules
• Olfactory Pit • Pronephric duct
• Otic vesicle • Mesomere
• • Visceral arch

3. Paste in the box for Figure 1 select sections showing the different structures identified in the 4 mm
frog embryo. Put appropriate labels for the noted structures. Use a clean copy of the box for Figure
1 if additional space is needed.
4. Fill up Table 1 and describe the fate of the structures identified in the 4 mm embryo. Use a clean
copy of Table 1 if additional space is necessary.

B. 10 mm Frog Embryo

1. Examine pictographs of serial sections of the 10 mm frog embryo. Start with the most anterior section
and proceed up to the most posterior section. As in the 4 mm embryo, it would be best to follow organs
and organ systems one at a time.
2. Take note of and identify the following structures (if present) in the serial sections. You may use the
following list as a checklist.

•Prosencephalon • Spinal cord • Pericardial coelom


•Telencephalon • Dorsal root • Endocardium
•Telocoel • Ventral root • Myocardium
•Olfactory nerve • Ependymal layer • Trabeculae carnae
•Nares (external, • Marginal layer • Ventral aorta
•internal) • Spinal canal • Dorsal aorta
•Diencephalon • Stomodeum • Internal carotid artery
Diocoel Proctodeum Dermatome
• • •
Epiphysis Tuberculum posteriosus Myotome
• • •
Infundibulum Internal gills Sclerotome
• • •
Rathke’s pocket Gill rakers Mesonephros
•Optic nerve • Opercular cavity • Mesonephric duct
•Optic cup • Velar plate • Splanchnic mesoderm
•Optic chiasma • Tongue • Somatic mesoderm
•Optic recess • Pharynx • Coelom
•Lens • Esophagus • Mesentery
•Pigmented retina • Stomach • Peritoneum
•Sensory retina • Intestine • Peritoneal cavity
•Cornea • Liver • Lymph sinus
•Anterior chamber • Lung buds • Mesenchyme
•Vitreous chamber • Thyroid • Cranial cartilage
•Optic cup • Trachea • Visceral arch (#1, #2, #3,
•Optic nerve • Hyomandibular pouch • #4)
•Mesencephalon • Posterior cardinal veins
•Mesocoel • Anterior cardinal veins
Rhombencephalon Common cardinal veins
• •
Rhombocoel Sinus venosus
• •
Otic vesicle Atrium
•Metencephalon • Ventricle
•Metacoel • Conus arteriosus
•Myelencephalon • Truncus arteriosus
•Myelocoel • Pericardium
•Posterior choroid •
•plexus •

3. Paste in the box for Figure 2 select sections showing the different structures identified in the 10
mm frog embryo. Put appropriate labels for the noted structures. Use a clean copy of the box for Figure 2
if additional space is needed.

4. Describe the origin of the structures identified in the 10 mm embryo. Fill up Table 2 by listing down
the structures noted and placing a check under the column(s) for the germ layer(s) from which each
structure was derived. Use a clean copy of Table 2 if additional space is necessary.
Figure 1. Select sections of the 4 mm frog embryo

OPTIC CUP
PROSENCEPHALON

RHOMBENCEPHA
OLFACTORY PIT LON HYPOPHYSIS

NOTOCHORD
STOMODEUM

HEART

INFUNDIBULUM

HYPOPHYSIS
FOREGUT

SPINAL CORD

HEART
ADHESIVE GLAND SOMITE
HEAD PHARYNX

LIVER

RHOMBENCEPHALON

FOREGUT

NOTOCHORD

PHARYNX
Table 1. Fate of structures identified in the 4 mm frog embryo

Structure/organ rudiment Fate in 10 mm embryo Fate in adult

Prosencephalon

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