RESEARCH CONFERENCE

ESF-EMBO Symposium

Biological Surfaces and Interfaces

Hotel Eden Roc, Sant Feliu de Guixols (Costa Brava)  Spain
June 26 – July 1, 2011

Chair: Fredrik Höök, Chalmers, Sweden

Co-Chair: Janos Vörös, ETH Zürich Switzerland

Vice-Chairs: Ralf Richter, CIC biomaGUNE, Spain

Eva Sinner, BOKU Vienna, Austria

© Patric Wallin, Chalmers University

WITH SUPPORT FROM

www.esf.org
ESF-EMBO Symposium 26 June - 1 July 2011

Biological Surfaces and Interfaces

Final Programme
Sunday 26 June
17:00
Registration at the ESF Desk
onwards
19.00 Welcome Drink
20.00 Dinner

Monday 27 June
Session 1: Engineering BioInterfaces
Chaired by: Fredrik Höök
08:45 – Fredrik Höök, Chalmers, SE and Janos Vörös, ETH-Z, CH
09:00 Welcome and Information
Andreas Janshoff, Göttingen University, DE
09:00 - 09:45
Local Membrane Mechanics: From Supported Bilayers to Living Cells
Catherine Picart, Grenoble-Institute of Technology and CNRS, FR
09:45 - 10:30
Engineering of Layer-by-Layer Films to Control Cell Differentiation
10.30 - 11.00 Coffee Break
Stefan Zauscher, Duke University, Durham, US
11.00 - 11.45 Surface-Tethered (Bio)Macromolecular Nanostructures for Nanomechanical
Sensing and Novel Detection Platforms
Matthias Lutolf, EPFL Lausanne, CH
11:45 - 12.30
Engineering Stem Cell-Niche Interfaces
12:30 Lunch and Free Time
15.45 - 16.15 Coffee Break
Session 2: Cells at Interfaces
Chaired by: Janos Vörös
Mattis Riehle, University of Glasgow, UK
16.15 - 17.00
Engineering cellular function in 2 and 3D
Joachim Rädler, Ludwig-Maximilians-Universität Munich, DE
17.00 - 17.45 Lipid Coats for siRNA, Silica Nanoparticles and Addressable Single Cell
Assays
Rafi Korenstein, Tel Aviv University, IL
18.00 - 18.45
Nanoscale Fluctuations of the Cell Membrane
19.00 - 20.30 Dinner
Key Note Lecture l, chaired by: Marcus Textor
20.30 - 21.30 Buddy Ratner, University of Washington, US
Blood Compatibility Driven by the Biointerface: Old Ideas
21.30 Poster Session l
Tuesday 28 June
Session 3: Nanomedicine
Chaired by: Eva Sinner
Ole Mouritsen, MEMPHYS - Center for Biomembrane Physics, University of
09.00 - 09.45 Southern Denmark, DK
Lipids and Liposomes for Nano-Medicine
Brigitte Städler, Aarhus University iNano, DK
09.45 - 10.30
Subcompartmentalized Systems for Cell Mimicry and Drug Delivery
10.30 - 11.00 Coffee Break
Tanja Weil, Ulm University, DE
11.00 - 11.45
Protein-derived Copolymers as Biocoatings and for Drug Delivery Applications
Short Oral Presentations:
Chaired by: Eva Sinner
Derda Ratmir, University of Alberta, US
11.45 - 12.00 High-throughput Assembly and Analysis of 3D cultures using Cells in Gels in
Paper
Jennifer Saik, Rice University, US
12.00 - 12.15 Rapid Anastomosis between Encapsulated Cells in Hydrogels and Host
Vasculature
Yajun Weng, Southwest Jiaotong University, CN
12.15 - 12.30 Immobilization of Bioactive Molecule on TiO2 for in situ Catalytic Generation
of Nitric Oxide
12.30 Lunch and Free Time
15.45 - 16.15 Coffee Break
Session 4: Sponsor Presentations
Chaired by: Fredrik Höök
Biolin Scientific - Presented by Vijay Gupta
16.15 - 16.30 Leading Provider of Analytical Instruments for the Nano-Scale and Molecular
Study of Interfaces and Interaction on Surfaces
Microvacuum - Presented by Istvan Szendro
16.30 - 16.45
Label Free Optical Biosensors Combined with Electrical Measurements
JPK Instruments AG - Presented by Carmen Pettersson
16.45 - 17.00
A Force Toolkit for Cell and Molecular Research
Poster Session l cont. and Drinks Reception
17.00 Courtesy of JPK Instruments AG

19.00 - 20.30 Dinner

Key Note Lecture ll, chaired by Helmuth Moehwald
Marcus Textor, ETH Zürich, CH
20.30 - 21.30
Self-assembly of Functional Polymers to Interfaces for Applications in Biology
and Medicine
21.30 Poster Session l cont. and II starting
Wednesday 29 June
Session 5: Lipid-Based Biointerfaces
Chaired by: Catherine Picart
Claudia Steinem, Göttingen University, DE
09.00 - 09.45
Pore-Spanning Membranes: Generating Functional Nanocompartments
Paul Cremer, Texas A&M University, US
09.45 - 10.30
Detecting Biomolecules by Local pH Modulation
10.30 - 11.00 Coffee Break
Nam-Joon Cho, Stanford University, US
11.00 - 11.45 Viral Peptide Isolated from the Hepatitis C Virus (HCV): Interactions with Lipid
Assemblies and Biotechnological Applications
Short Oral Presentation:
Chaired by: Janos Vörös
Pablo Dörig, ETH Zurich, CH
11.45 - 12.00
Single Cell Displacement and Injection: Application of FluidFM Technology
Sponsor Presentations:
Nanolane - Presented by Rémi Corso
12.00 - 12.15 SARFUS : Access to the Nanoworld Simply with a Reflection Optical
Microscope
LayerLab - Presented by Olof Anderssen
12.15 - 12.30 Resonance Enhanced Impedance Measurements of Lipid Bilayer Formation
and Ion-channel Mediated Charge Transport
12.30 Lunch
14.00 Excursion - Guided Tour of Sant Feliu de Guixols
19.00 - 20.30 Dinner
Key Note Lecture lll, chaired by Buddy Ratner
20.30 - 21.30 Helmuth Moehwald, Max Planck Institute of Colloids and Interfaces, DE
From Peptide-Membrane Interactions to Remote Release
Poster Session lI cont. and Open Bar
21.30
Courtesy of LayerLab
Thursday 30 June
Session 6: Small-Scale Analysis
Chaired by: Andreas Janshoff
Toshio Ando, Kanazawa University, Kanazawa, JP
09.00 - 09.45 Direct and Dynamic Visualization of Protein Molecules in Action by High-
Speed AFM
Christian Eggeling, MPI Göttingen, DE
09.45 - 10.30
Studying the Membrane with STED Fluorescence Nanoscopy
10.30 - 11.00 Coffee Break
Peter Sjövall, Technical Research Institute of Sweden, SE
11.00 - 11.45
Molecular Mapping of Biological Surfaces and Interfaces with TOF-SIMS
Jongyoon Han, MIT, US
11.45 - 12.30
Microfluidic Tools for Single Cell Measurement and Activation
12.30 Lunch and Free Time
15.45 - 16.15 Coffee Break
Session 7: Short Oral Presentations
Chaired by: Ralf Richter
Maxim Nikitin, Prokhorov General Physics Institute of Russian Academy of
Sciences, RU
16.15 - 16.30
Self-Assembly of Multifunctional Structures and Novel Methods for their
Quantitative Detection in Vivo
Marta Bally, Chalmers University of Technology, SE
16.30 - 16.45 Interactions of Norovirus Virus-like Particles with Cell-Membrane Mimics
Containing Glycosphingolipids
Sang-Hyun Oh, University of Minnesota, US
16.45 - 17.00 Template-stripped Smooth Metallic Nanostructures for Microfluidic Biosensing
and Spectroscopy
17.00 Poster Session II cont.
Forward Look Session
Chaired by: Ralf Richter
19.00 - 20.00
Session Panel: Pep Pàmies, Paul Cremer, Marcus Textor, Buddy Ratner, Ole
Mouritsen and Claudia Steinem
20.00 Drinks Reception and Conference Dinner

Friday 1 July
Breakfast and Departure
 With Support from:
RESEARCH CONFERENCES

ESF-EMBO Conference on Biological Surfaces and Interfaces

26 June – 1 July 2011, Sant Feliu de Guixols, Spain
Soft Matter
List of Posters
SESSION ONE
1 Forschungszentrum Jülich
Afanasenkau Dzmitry Supported lipid bilayers as a substrate for neuronal cell culture
GmbH
2 Chalmers University of
Altgärde Noomi Supported Lipid Membranes as a Platform for Carbohydrate Interactions
Technology
3
An Qi University of Twente Immobilization of proteins onto surfaces with supramolecular ways
4
Ardhaoui Malika University College Dublin Functionalised carbon bioelectrode for laccase-catalyzed oxygen reduction by direct electron transfer
5 Max-Planck-Institute for Metals Forces and mechanical properties of hyaluronan films - A study combining colloidal probe atomic force microscope and reflection
Attili Seetharamaiah
Research interference contrast microscopy
6
Baranova Natalia CIC biomaGUNE Towards the assembly of an artificial cumulus matrix – a study on a surface-based model system
7 Academy of Sciences of the
Bartosik Martin Electrochemical detection of RNA using osmium complex as a specific electroactive label
Czech Republic
8 Studying the supramolecular interactions of the extracellular matrix components versican and hyaluronan with a surface-based
Bernecker Anja CIC biomaGUNE
model system
9
Billiet Thomas Ghent University Crosslinking of gelatin matrices for cell encapsulation
10
Blanford Christopher University of Manchester Engineered electrode surfaces for biomimetic attachment of oxygen-reducing enzymes
11 Institute of semiconductor Layered interfacial architectures on SAM-modified gold surfaces based on macrocyclic Ni(II) complex and biphenyl-4,4'-
Boltovets Praskoviya
physics dicarboxylate
12
Chandrawati Rona The University of Melbourne Capsosomes: Enzyme-Loaded Liposomal Subcompartments within Polymer Carrier Capsules
13 Surface biomimetic modification of titanium oxide film by biomolecular immobilization for antithrombogenic and
Chen Junying Southwest Jiaotong University
endothelialization
14
Custódio Catarina University of Minho Photopatterned chitosan surfaces for spatially controlled immobilization of different cell types
15 Chalmers University of
Czolkos Ilja Micropatterned Teflon AF as a Substrate for Biomaterial Studies
Technology
16
De Groot Wilma Universitiy of Twente Smart polymer brushes for functionalization of platforms for membrane protein screening
17
De Lange Victoria ETH Zurich A Simple Approach to Array Fabrication: Producing Multiple Biochip Copies from Functionalized Hydrogel Channels
18 Université Catholique de
Degand Simon Design of surfaces with mechanical nano-heterogeneities for a better control of cell-material interactions
Louvain
19
Dekany Imre University of Szeged Plasmonic Properties of Biofunctionalized Gold/Silver Nanoparticles at different pH in Aquatic Dispersions
20 A Simple and Versatile Approach to Design Oligoethylene based Self Assembled Monolayer on silicon using Thiolene Chemistry:
Dellinger Antoine Chimie ParisTech (ENSCP)
preparation and surface characterisation
21 Swiss Federal Institute of
Demarche Sophie In-situ reconstitution of proteobilayer in nanopores
Technology (ETHZ)
22
Dermutz Harald ETH Zurich Physiological neuron networks and their functional capabilities
23 Laboratory of Biosensors &
Dielacher Bernd Combining optical and electrical biosensing with novel metal nanowire arrays
Bioelectronics, ETH Zürich
24 A nanoscopic model system based on a biointerface to investigate the mechanism of transport selectivity in nucleo-cytoplasmic
Eisele Nico CIC biomaGUNE
protein exchange
25
Fattinger Christof F. Hoffmann-La Roche Ltd Exploiting Molecular Biology by Time-Resolved Fluorescence
26
Floyd Alaina University of Washington Formation and Characterization of Drug Encapsulated Polymeric Microspheres for Localized Brain Tumor Therapy
27
Fourel Laure INP Grenoble Presentation of BMP-2 from a soft biopolymeric film unveils its activity on cell adhesion and migration
28
Franco Davide ETH Zurich Control of initial endothelial spreading by topographic activation of Focal Adhesion Kinase
29
Gaus Katharina University of New South Wales Spacing of integrin ligands influences signal transduction in endothelial cells
30 Mihaela Max Planck Institut for Polymer
Gropeanu Photosensitive His taggs for spatiotemporal control of protein binding to surfaces
Gabriela Research
31 SP Technical Research Institute Biorecognition at cell membrane surfaces-novel time-resolved assays for kinetic studies of membrane receptor-ligand
Gunnarsson Anders
of Sweden interactions
32
Gupta Swati CIC biomaGUNE Role of Ca in platelet interactions with foreign surfaces
33
Hanley Luke University of Illinois at Chicago Mass Spectrometric Imaging of Antibiotics and Proteins within Intact Bacterial Biofilms
34
Haylock David CSIRO Perfusion based bioreactors for megakaryocyte growth and platelet production
35 Chalmers University of
He Wenxiao Liquid crystal templating of nano-calcium phosphates
Technology
36
Hill Ryan Toler Duke University Plasmon Resonant Nanoparticles Coupled to Metal Film as a Biosensing Platform
37
Hortiguela Maria Jesus NUI Galway Monolithic silica columns for environmental monitoring applications
38 Synthetic Protein Targeting by the Intrinsic Biorecognition Functionality of Poly(ethylene glycol) using PEG-Antibodies as
Hyötylä Janne University of Basel
Biohybrid Molecular Adaptors
39
Inci Fatih Istanbul Technical University Characterization of Tethered Bilayer Lipid Membranes by Quartz Crystal Microbalance-Dissipation (QCM-D)
40
Irbe Zilgma Riga Technical University Cell adhesion behavior on α-tricalcium phosphate Bone Cements
41 Chalmers University of
Irep Gozen Fractal Avalanche Ruptures In Biological Membranes
Technology
42 Tampere University of
Jaatinen Leena Controlling Cell Migration and Adhesion in a Three-dimensional Scaffold by External Electric Currents
Technology
43
Jagoda Agnieszka University of Basel Polymer-inorganic composite materials for bone tissue engineering
44
Johnson Patrick University of Wyoming Colloidal SERS Bioassays for Point of Care Diagnostics
45 Leibniz Insitute of Polymer
Kaufmann Martin Lipid Bilayers on Responsive Polymer Cushions for Membrane Protein Reconstitution
Research Dresden
46
Kluger Petra Fraunhofer Gesellschaft Generation of optimized culture surfaces for primary human cells by (bio)chemical and topographical modifications
47
Kokol Vanja University of Mairbor Interactions between cells and 3D engineered gelatin-based scaffolds
48 Max Planck Institute for
Kresak Slavoj Giga-seal solvent-free bilayer lipid membranes: from single nanopores to nanopore arrays
Biophysics
49
Kung Kuan-Chen National Cheng Kung University Effects of ordered texture surface with nanometric scale roughness on biological responses for titanium implants
50 Russian Academy of Sciences
Kuvichkin Vasily Lipid bilayers aggregation and fusion in complexes of DNA- divalent metal cations – zwitterionic liposomes
(RAS)
SESSION TWO
51
Leal-Egaña Aldo University of Leipzig Does the liver tune elasticity and biodegradability at the cell-matrix interface to control primary tumours?
52
Maiolo Daniele University of Brescia Study of Surface Confined Angiogenic Ligand Receptor Complexes by Contact Angle Molecular Recognition
53 Swiss federal Institute for
Maniura Katharina Cell shape versus cytoskeleton integrity: probing the re-expression of the chondrogenic phenotype
Materials Testing and Research
54 Université Catholique de
Matos Ruiz Mireya Design of biocompatible nanofilms for Nitinol surfaces
Louvain (UCL)
55 Commonwealth Scientific and
Meagher Laurence Biomedical applications of living, surfaace initiated polymerisation: a platform approach
Industrial Research
56 Grazia Maria
Messina University of Catania Peptide trapping in Nanopores for Antibacterial Surfaces
Lucia
57
Mhanna Rami ETH Zurich Engineering Cartilage Extracellular Matrix Mimics for Optimal Chondrocyte Culture
58
Montero Laura Universitat Ramón Llull Initiated Chemical Vapor Deposition (iCVD) of ultrathin thermally responsive films to prevent Biofilm formation
59 Leibniz Institute of Polymer
Müller Eike Micropatterning of ECM-inspired Hydrogels
Research Dresden
60
Ng Sher Leen The University of Melbourne Polymer Capsules as Drug Delivery Vehicles: Tunable Carrier Degradation and Cargo Release
61
Nilebäck Erik Q-Sense Cytoskeletal rearrangements of fibroblasts measured simultaneously by QCM-D and light microscopy
62 Adam, Lars, University Medical Center
Olsson Acoustic sensing of biocolloid-substrate bond stiffness
Johan Groningen
63
Ossola Dario ETH Zürich Towards automated force-controlled gigaseal formation for electrophysiology measurements using the FluidFM technology
64
Pribyl Jan Masaryk University Artificial surfaces based on surface-immobilized hyaluronate for controlled adhesion of sperms
65 Chalmers University of
Rabe Michael Aggregation of alpha-Synuclein on biological interfaces starts at nanomolar concentrations
Technology
66 University of Wisconsin
Renner Lars David The Role of Cardiolipin Domains in Protein Localisation in Bacterial Cells
Madison
67 The University of the Basque
Reviakine Ilya Phosphatidyl serine containing liposomes on titania: phase behaviour, bilayer formation, and lipid asymmetry
Country
68
Ribeiro Nilza University of Porto SPARC adsorption on nanophased hydroxyapatite and its influence on MC3T3-E1 osteoblast adhesion and morphology
69 Warsaw University of
Roguska Agata Bioactive TiO2 nanotube layers with antibacterial properties
Technology
70
Rupert Deborah Chalmers Technical University Microfluidics biosensors for small volume low concentration biosamples: multi-dimensional fluorescence accumulation
71
Santonicola Mariagabriella Italian Institute of Technology Functional supported biomembranes on surface-grafted polymer architectures
72 Max-Planck Institute for
Shafiq Zahid DOPA Crosslinked Biopolymers
Polymer Research
73 Paracrine Interactions between Mesenchymal Stem Cells Affect Substrate Driven Differentiation Toward Tendon and Bone
Sharma Ram ETH Zurich
Phenotypes
74 Chalmers University of
Simonsson Lisa Lipid vesicle fusion for studies of cell functions
Technology
75
Singh Kulveer University of Manchester Inactivation processes of immobilised redox enzymes studied by an electrochemical quartz crystal microbalance
76
Sinner Eva-Kathrin BOKU Wien University Proteins in Plastic
77
Spycher Philipp ETH Zurich Patterning of Supported Lipid Bilayers to Guide Neuronal Cell Growth
78 Georg August Universitaet
Stephan Milena Development of a Biomembrane Sensor Based on Reflectometry
Goettingen
79
Strancar Janez Jozef Stefan Institute Understanding the cancer-cell’s uptake of the nano-sized particles via fluorescence microspectroscopy
80
Sugihara Kaori ETH Zurich Lipids on polyelectrolytes for biosensing and biomaterials
81 Tokyo Women's Medical
Tamura Atsushi Design of stimulus-sensitive cell culture microbeads for large-scale cultivation of therapeutic cells
University
82
Tejero Ricardo Eduardo Anitua Foundation Blood plasma on TiO2: Time of Flight Secondary Ion Mass Spectroscopy and Principal Component Analysis study
83
Trouche Elodie CIC BiomaGUNE How Activation with Plasma Rich in Growth Factors Influences Immune Response to Titanium Implants
84
Vecbiskena Linda Riga Technical University (RTU) Tricalcium phosphate bone cements: Synthesis, phase composition and in vitro biocompatibility
85 Consejo Superior de
Velez Marisela Modulating Self Assembly of Bacterial Cytoskeletal Protein FTSZ on Lipid Bilayers
Investigaciones Científicas
86 Fraunhofer Institut für
Volodkin Dmitry Microfluidic patterning of Layer-by-Layer assembled films for selective cell growth
Biomedizinische Technik
87
Wall Gerard NUI Galway Regeneration of the inter-vertebral disc region using a targeted gene delivery approach
88
Wasserberg Dorothee University of Twente Orientationally Controlled Immobilization of Visible Fluorescent Proteins
89 Max Planck Institute for
Weis Simone Novel caged RGDs for photocontrol of cell attachment to artificial surfaces
Polymer Research
90 Chalmers University of
Westas Emma Analysis of Staphylococcus epidermidis biofilm formation on nanostructured hydroxyapatite surfaces
Technology
91
Willison Keith Robert Institute of Cancer Research Ultrasensitive quantitative proteomics by microfluidic single molecule analysis
92
Yen-Ting Liu National Cheng Kung University Dissection of SAM modified TiO2 nano structure and its bioactivity
93
Zambelli Tomaso ETH Zurich FluidFM technology and single-cell biology: from single-virus infection to bacterial adhesion