DYNEXr Manual, Rev A
DYNEXr Manual, Rev A
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Please read carefully before using the MRX Microplate Reader
Software Version:
Instrument Nº:
This document is the copyright of and must not be copied or reproduced in any
form without prior consent.
DYNEX Technologies reserves the right to make technical improvements to this equipment and
documentation without prior notice as part of a continuous program of product development.
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MRX is a trademark of .
®
MRX is a registered trademark of .
® ® ®
Microsoft , Excel , and Windows are registered trademarks of Microsoft Corporation.
Limitations of Use
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The user of the MRX must follow the specific manufacturer’s assay package insert when modifying
parameters and establishing results calculation methods.
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The performance characteristics of the MRX have not been established with specific serology or antigen
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detection assays. The user must evaluate the MRX in conjunction with the specific serology and/or
antigen detection assays. This evaluation must include the establishment of performance characteristics
for the specific assays.
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Under no circumstances does the MRX have any direct patient contact or perform any therapeutic
patient function.
Specific diagnostic results are not provided by the device. Diagnostic decisions are made only after
independent confirmation by additional methods under the supervision of a qualified professional.
WARNING:
If this equipment is used in a manner not specified by the manufacturer then the
protection provided by the equipment may be impaired.
Before using any decontamination method, except those recommended by the
manufacturer, users should check with the manufacturer that the proposed method will
not damage the equipment.
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Table of Contents
Table of Contents
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MRX Microplate Reader User Manual
Electrical .................................................................................................. 33
Physical/Environmental Conditions......................................................... 35
Index.........................................................................................36
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About This Manual
This manual has been written for the laboratory technician and describes
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how to use the MRX Microplate Reader.
With the information in this manual, you can:
• Install the Reader.
• Configure the Reader, using the Setup dialog, to suit your particular needs.
• Connect the Reader to a PC (personal computer).
• Perform basic maintenance procedures.
This manual also describes all the features and specifications of the
Reader hardware and software.
Information on how to use the Revelation software is included.
For explanations of error messages and possible solutions, refer to the
included Troubleshooting Guide.
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MRX Microplate Reader User Manual 1
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2 MRX Microplate Reader User Manual
Safety Information
Safety Information
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MRX Microplate Reader User Manual 3
Safety Information
• MAKE SURE that the mains power cable is correctly wired. Color codes
are as follows:
Europe United States
Brown Live Black Live
Blue Neutral White Neutral
Green/yellow Earth (ground) Green Ground
Fuses The instrument does not contain any user-replaceable internal fuses.
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Symbols
Symbols
Warning Symbol
For your safety the Reader is marked with a warning symbol to remind
you of certain hazards:
!
• On the back panel: this indicates that the optics door may be hot. If you
want to remove the optics door, make sure that you allow the Reader to
cool before touching it.
• Adjacent to the mains outlet: This indicates that the line voltage switch
must be correctly set before plugging the unit into an outlet. This also
indicates that the auxilliary outlet has a power rating of up to 120VA and an
earth leakage current of less than 1 mA at 264V and 60 Hz.
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6 MRX Microplate Reader User Manual
Chapter 1 Features of the Reader
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The MRX Microplate Reader is a microprocessor-controlled
photometer. It is designed to measure the optical density (OD) of fluid
samples in 96-well microplates in order to determine the results of
chemical reactions.
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Figure 1 MRX Microplate Reader
A full list of the Reader’s technical specifications can be found in
Appendix C.
The Reader provides the following features:
• Revelation software capable of detecting Endpoint, Agglutination and
Kinetic assays
• PC-compatibility for control and data storage
• Single, dual, and multi-wavelength reading modes
• Selection of up to six filter wavelengths
• Data analysis and presentation as OD matrices and/or graphics using curve
fitting routines
• Data output to a PC
• Verification Plate option
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Chapter 1 Features of the Reader
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Chapter 2 Installation and Setup
2.1 Installation.......................................................................................... 11
Unpacking the Reader .......................................................................... 11
The Reader Back Panel ........................................................................ 11
Voltage Selector Switch ....................................................................... 13
USB Driver Installation........................................................................ 13
The Reader Front Panel........................................................................ 15
Fitting the Lamp and Optical Filters .................................................... 15
Switching the Reader On...................................................................... 17
Self-Tests……………………………………………………..…...…….…………11
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Chapter 2 Installation and Setup
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Chapter 2.1 Installation
2.1 Installation
CAUTION DO NOT connect the instrument to the mains electrical supply or
switch it on before you have completed this installation procedure.
3
2
4
6
7
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Chapter 2.1 Installation
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The MRX Back Panel
4 7 9
1 5
2 3
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Figure 3. The MRX Back Panel
1 Mains Inlet 6 Optics Cooling Vents
2 Voltage Selector 7 Serial Number Plate
(See the Voltage Selector
section below)
3 Aux Mains Socket 8 PSU Cooling Vents
4 USB Port 9 Main Enclosure
5 Optics Door
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Chapter 2.1 Installation
To start the installation process open the file USB Driver and VCP Install
Procedure located on the supplied User CD. Follow the procedure through to
its completion before continuing.
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Chapter 2.1 Installation
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Chapter 2.1 Installation
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The MRX Front Panel
3 2 1
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Chapter 2.1 Installation
Figure 6 Correct Filter Spring Positioning (view of Filter Wheel from filter
spring side)
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Chapter 2.1 Installation
4. Slide the optics assembly back in, attach the lamp connector to the
lamp, and replace the optics chamber door.
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MRX Microplate Reader User Manual 17
Chapter 2.1 Installation
Self-Tests
When the Revelation program starts on the computer the unit carries out
several self-tests to check that the instrument is working correctly.
Appendix A shows a list of these tests and a sample printout.
If the Reader fails any of the tests, an error message is displayed. If the
software can not communicate with the Reader it will request the unit’s
serial number (this can be found on the Reader’s back panel). Double
check the cable connection between the Reader and the PC. If an error
message persists, contact the service center.
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Chapter 2.1 Installation
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MRX Microplate Reader User Manual 19
Chapter 3 Maintenance
Chapter 3 Maintenance
3.1 Routine Maintenance Procedures ...................................................... 22
3.2 Cleaning ............................................................................................ 23
Autoclaving .......................................................................................... 23
External Painted and Plastic Surfaces .................................................. 23
Filters.................................................................................................... 23
Decontamination .................................................................................. 23
3.3 Repacking the Reader ....................................................................... 24
3.4 Replacing the Lamp .......................................................................... 25
3.5 Changing the Optical Filters ............................................................. 26
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Chapter 3 Maintenance
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MRX Microplate Reader User Manual 21
Chapter 3 Maintenance
IMPORTANT
The first section in this manual contains safety information for all
the equipment in the system. You should be familiar with this
information anyway, but if not, you must read through this
section before carrying out any maintenance.
The warranty on the Reader may become void if you do not
follow the safety and maintenance instructions contained in this
manual, or if the covers are removed by anyone other than
approved service personnel.
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3.1 Routine Maintenance Procedures
3.2 Cleaning
Warning: Always switch the instruments off and disconnect the power cable
before cleaning the Reader.
The Reader is constructed from high quality materials, nevertheless spilt
saline solutions, solvents, acids or alkaline solutions must be removed
from outer surfaces immediately to prevent damage.
Autoclaving
DO NOT autoclave any part of this instrument.
Filters
Clean the filters by wiping them with a lint-free cloth or a lens tissue. If
necessary a little alcohol may be used with the cloth or tissue.
Decontamination
If you need to return the Reader to the service center, and it has been in
contact with human blood, other potentially infectious body fluids,
pathological samples, toxic or radioactive materials, it must be
decontaminated.
Before shipping the instrument, do the following:
• Clean the surface with a mild laboratory detergent followed by 10%
bleach.
• Fill out an Equipment in Transit form (refer to Appendix B for a
copy of the form).
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MRX Microplate Reader User Manual 23
3.2 Cleaning
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3.3 Repacking the Reader
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3.5 Changing the Optical Filters
Note: DO NOT touch the filters, lamp or reflector with bare fingers.
1. Switch off the power supply and disconnect the mains cable.
2. Using a Phillips screw driver remove the optics door.
3. Slide the optics assembly out of the instrument.
4. Remove the relevant filter from its position in the filter wheel, and
replace with the new one.
• Make sure the side of the filter that has the shorter distance from the
groove to the end is toward the lamp.
• Make sure the Filter Springs are around the sides of the Filter as shown in
Figure 6 on page 14. The Springs must not extend past the rear face of
the Filter as shown in Figure 7 on page 15. Otherwise, the self-test will
fail due to “Filter Motor Errors.”
5. Slide the optics assembly back into the Reader chassis.
6. Replace the optics chamber door and reconnect the power supply.
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3.5 Changing the Optical Filters
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Appendix A: Self-Test Diagnostics
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Appendix A: Self-Test Diagnostics
SELF-DIAGNOSTIC SEQUENCE
Date : 3/17/98
Time : 11:15:02 AM
Self-Diagnostic Summary
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Appendix B: Accessories
Appendix B: Accessories
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Appendix B: Accessories
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Appendix C: Specifications
Appendix C: Specifications
Software Specifications
e Standard: Revelation MRX Program
MRX
Hardware Specifications
No. of filters 6
Performance
Read cycle time Single Wavelength: < 4 seconds
Dual Wavelength: < 6 seconds
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Appendix C: Specifications
† Linearity is the maximum percentage deviation of a reading from the ideal straight
line plot of OD versus concentration. (Measured by the manufacturer at 410nm in the
visible range and 340nm in the UV range).
* Precision is the mean Coefficient of Variation (CV) figure for all wells on a plate that
has been read 20 times in succession. (Measured by the manufacturer at 410nm in the
visible range and 340nm in the UV range).
Interface
Computer Interface USB 1.0/2.0 compatible
Electrical
Light source 75W tungsten halogen lamp
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Appendix C: Specifications
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Appendix C: Specifications
Physical/Environmental Conditions
Weight 10.kg (22.lb)
Dimensions D390 x W360 x H220 mm
(15.4 x 14.2 x 8.7 inches)
Operating Temperature 10 – 40°C
Operating Humidity 10 – 90% RH
Storage Temperature 0 – 50°C
Storage Humidity 10 – 90% RH
Altitude < 2000m
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Appendix C: Specifications
Index
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MRX
—A— back panel, 12
Accessories, 29
—O—
—B— Optical filters
changing the, 25
Back Panel, 12 Optical Filters
fitting the, 14–16
—C—
Cleaning, 22 —R—
Reader
—D— accessories, 29
electrical specifications, 32
Decontamination, 22 features of, 7–8
hardware specifications, 31
interface, 32
—E— performance specifications, 31–32
Electric Shock Hazard, 3 physical/environmental conditions, 34
Equipment in Transit form (decontamination). repacking the, 23
See Appendix B self-tests, 17
software specifications, 31
External Painted and Plastic Surfaces
switching it on, 16
cleaning the, 22
unpacking the, 11
Repacking the Reader, 23
—F—
Filters —S—
cleaning the, 22
Safety Information, 3–4
Front Panel, 14
Self-tests, 17, 27
Fuses, 4
sample printout, 28
Specifications
—I— electrical, 32
hardware, 31
Incorrect Operation, 3 interface, 32
performance, 31–32
—L— physical/environmental, 34
software, 31
Lamp
fitting the, 14–16
replacing the, 24
—V—
Voltage Selector, 13
—M—
Maintenance, 21–22 —W—
Microplate Reader. See Reader. Warning Symbol, 5
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MRX
front panel, 14
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