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The Laboratory Manual:: Contents and Development

The document discusses the importance of developing a laboratory manual and provides guidance on its contents and development. Key points: - A manual ensures safety guidance and training, documents procedures clearly for all technicians, and provides a written record of inherent risks. - The manual should be site-specific, reviewed annually or when procedures change, easily accessible in the lab, and maintained by a responsible person. - Contents may include agent inventory, emergency contacts, risk assessment, standard operating procedures, training requirements, and emergency response plans. The manual helps ensure lab procedures are appropriate and clearly understood.

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Barnoota Komputeraa afaan oroomon
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225 views

The Laboratory Manual:: Contents and Development

The document discusses the importance of developing a laboratory manual and provides guidance on its contents and development. Key points: - A manual ensures safety guidance and training, documents procedures clearly for all technicians, and provides a written record of inherent risks. - The manual should be site-specific, reviewed annually or when procedures change, easily accessible in the lab, and maintained by a responsible person. - Contents may include agent inventory, emergency contacts, risk assessment, standard operating procedures, training requirements, and emergency response plans. The manual helps ensure lab procedures are appropriate and clearly understood.

Uploaded by

Barnoota Komputeraa afaan oroomon
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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The  Laboratory  
 
Manual:  
 
Contents  and  
Development  

Ephy  Khaemba  
 
Manager,  EOHS  /  Research  Compliance,  ILRI  
 
Why  should  a  lab  develop  a  Laboratory  
Manual?  
•  Inherent  risks  of  laboratory  
materials  
•  Safety  Guidance  and  Training  
resource  
•  Provides  wriFen  documentaGon  
•  Ensure  lab  procedures  are  
appropriate  and  clearly  
understood  by  all  technicians  
The  Manual  should  be…  
•  Site-­‐specific  
•  Reviewed  and/or  revised  annually  (or  when  there  
is  a  change)  
–  Procedures  
–  Equipment  
–  Organisms  worked  with  
•  Stored  and  easily  accessible  in  the  lab    
•  Be  the  responsibility  of  a  person  to  maintain  

A  “Living”  document  
Exercise  

•  Iden&fy  3  items  (topics,  contents,  SOPs,  etc.)  


that  should  be  included  in  your  laboratory’s  
Laboratory  manual  
Contents  of  a  Laboratory  Manual  
1.  Title  page     7.  Agent  Inventory  
2.  Agents  worked  with  and   8.  Engineering  Controls  
characterisGcs   9.  OperaGons  and  
3.  Emergency  Contact   Maintenance  
InformaGon   10.  Bio  and  Mixed  Waste  
4.  Risk  Assessment   Management  
5.  Lab  Procedures  and  SOPs   11.  AddiGonal  Safety  Hazards  
6.  OccupaGonal  Health   12.  Training  
Program   13.  Emergency  Procedures  and  
Incident  Response  
Example of a title page
Contents  of  a  Laboratory  Manual  
con?nued…  
1.  List  of  disinfectants  used  in   7.  Autoclave  usage  
lab   8.  Safety-­‐related  forms  
2.  Sharps  handling   9.  Signage  used  in  the  lab  
procedures   10.  Laboratory  resources  and  
3.  Bldg  evacuaGon   informaGon  
procedures   11.  Chemical  safety  
4.  Employee   informaGon  
acknowlegement     12.  RadiaGon  safety  
5.  Laboratory  definiGons   13.  Manual  review  and  
6.  Packing  and  Shipping   revisions  page  
guidance  
Lab  Procedures  
•  Standard  Microbiological  Techniques  
•  Special  PracGces  
•  Signage  used  within  the  lab  
•  Special  Policies  
–  Visitors  
–  Temporary  Employees  
–  Non-­‐scienGfic  staff  
SOPs  
•  Entry  and  Exit  
•  Safety  Equipment  
–  PPE  
–  Biological  Safety  Cabinets  
–  Containment  equipment,  i.e.  sealed  rotors  
•  Storage/Inventory  
•  Specimen  transport/packing/shipping  
•  OccupaGonal  Health  procedures    
SOPs  (cont’d)  
•  DecontaminaGon  
–  Disinfectants  used  in  lab  
–  Spills  
–  Equipment  
–  Space  DecontaminaGon  
•  Waste  Management    
–  Liquids  
–  Solids  
–  Sharps  
Exercise  

•  Choose  one  of  the  three  elements  from  your  


manual  and  create  an  outline  of  the  individual  
components  of  that  element  that  should  be  
included  in  the  manual    
Opera?ons  and  Maintenance  
•  Engineering  Procedures  
–  DirecGonal  Airflow  –  alarms  and  monitors  
–  Emergency  lighGng  
–  Generator  
•  Equipment  Maintenance  
–  BSCs,  autoclaves,  centrifuges  
–  ValidaGon/cerGficaGon  
•  System  Maintenance  
–  VenGlaGon  
–  Electrical  
–  Plumbing  
Addi?onal  Hazards  
Safety  procedures  for    
•  Chemicals:  Ethidium  bromide,  formaldehyde,  
bleach    
•  Physical:  UV  light,    
•  Ergonomics:  repeGGve  moGon,  BSC,  
•  RadiaGon    
Incident  Response  

Emergency  Response  
•  Spill  or  Release  of  a  pathogen  
•  Medical  Emergency    
–  Exposure,  heart  aFack,  fainGng,  etc.,  
•  Natural  Disaster  
–  fire,  earthquake,  etc.,    
   
Group  Exercise  

Develop and write an SOP for one of


the following:

•  Cleaning a bio-hazardous spill in a Laboratory


cabinet
•  Disposing of a broken blood tube found on the
laboratory floor
•  Procedures to follow if you have stuck yourself
with a potentially contaminated needle
What  We  Should  include  vs.    
What  We  Must  include  in  a  Manual  

•  “Musts”    (requirements •  “Shoulds”            (opGonal)  


)   –  CWA  15793  
–  RegulaGons   –  InternaGonal  Health  
–  Standards   RegulaGon  
–  Local  Laws   –  Best  PracGces  
–  InsGtuGonal  Policies   –  WHO  Manual  
PracGcal  MaFers  
•  Who  will  develop  the  manual?  
–  Who  is  responsible  for  deciding  on  the  specific  policies  and  
procedures  to  be  included?  
–  Who  reviews?  
•  Who  authorizes  the  manual?  
–  Establish  a  procedure  for  approving  the  manual  with  the  
support  of  the  insGtuGon’s  administraGon      
–  Who  do  you  include  in  the  process?  
•  QuesGon:  Must  all  laboratorians  sign  off  that  they  
have  read  and  understand  the  informaGon  
contained  in  the  manual?  
QuesGons?  

you !
hank
T

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