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Carl Smith - DNA Replication Activity

DNA replication is the semi-conservative process by which DNA copies itself for cell division. It involves unwinding the double helix, forming complementary base pairs between existing and new strands, and rewinding the double helix. Key enzymes that facilitate replication are DNA helicase, which unwinds the DNA; DNA polymerase, which adds new bases to the templates and connects nucleotides; and DNA ligase, which proofreads and connects the nucleotides of the new strands.

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619 views1 page

Carl Smith - DNA Replication Activity

DNA replication is the semi-conservative process by which DNA copies itself for cell division. It involves unwinding the double helix, forming complementary base pairs between existing and new strands, and rewinding the double helix. Key enzymes that facilitate replication are DNA helicase, which unwinds the DNA; DNA polymerase, which adds new bases to the templates and connects nucleotides; and DNA ligase, which proofreads and connects the nucleotides of the new strands.

Uploaded by

cj smith
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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DNA Replication

1. Number the steps of DNA replication in the correct order (1, 2, 3):
2 Daughter strands are formed using complementary base pairing.
1 DNA unwinds
3 The DNA of the daughter strands winds with together with its parent strand.
2. Why is DNA replication called "semi-conservative"?each
. strand contains one parent and one daughter stands

DNA helicase
3. What enzyme unwinds or unzips the parent strand?.
4. What enzyme connects the new bases to the old bases in the DNA template?
DNA polymerase

5. What enzyme connects the new nucleotides together and proofreads them?
DNA polymerase

6. Show the complimentary base pairing that would occur in the replication of the short DNA
molecule below. Use two different colored pencils (or different pens, markers, etc.) to show which
strands are the original and which are newly synthesized.

Original Original Original Original


DNA DNA DNA Strand New DNA +
New DNA DNA Strand
1 (copy from Strand Strand 2 (copy from
Strand 1 Strand 2
left) left)

+
A T A T
A T
C
+
C- G G
C G
+
T- A T A T
+
T- A T A T
A
-» +
A- T A T T
A
+
C- G C G
C G
+
G- C G C
G C
+
C- G C G C G
C +
C- G G C
1 G
+
G- C C C
G G
=__
+
A- T A
A T T

T- A T
+ —
A T
1 A

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