2-D Electrophoresis Solutions • Complete 2-D System that

Enables the Fastest Runs

with the Clearest Spots

• The Only 2-D System able

to Monitor Each Individual
IPG Strip in Real Time with
Graphical Display

IEF Unit

Complete 2-D
Packages

Large Vertical Unit

Mini Vertical Unit

Accessories

A Deluxe Vertical Unit

10250 Hoefer 6pg broch_2:Layout 1 9/22/09 4:25 PM Page 2

IEF100 First-dimension SE900 L

Isoelectric Focusing Unit Vertical
Monitor current flow for each individual IPG strip For highly
in real time separation
• 9 editable preloaded protocols included
• Can store up to 30 protocols The SE900 was d
electrophoretic se
• Flexible first-dimension IEF can be run with up to six 7 to 24 cm
which mirrors the
IPG strips simultaneously
accommodate pl
• Turn and click intuitive user interface with graphical display
• Capable of faster run times with an integrated 12,000 V power
supply that has the highest voltage and current commercially available Features and B
• Easily accessible Ethernet and RS232 ports No upper buffer ch
Utilizes minimal p
The IEF100 is the only first dimension instrument that controls the current
and voltage applied to the IPG strips to prevent overheating. External cooling an
and uniform temp
Features and Benefits
Up to six 28 cm pl
Cup-loading or rehydration-loading–versatility to accommodate individual throughput applic
sample requirements Glass plate sandwi
Instrument control through LAN–remote control and data acquisition possible assembly before u
Integrated power supply and Peltier cooling–minimizes footprint Built-in draining po
Constant power mode–minimizes overheating risks Assembly of the ta
Ultra high voltage and current–reduces focusing time and enhances Innovative design e
focusing results consumption for r
Focusing tray clamped to cooling plate–ensures efficient heat transfer
Electrodes lock into place on strips–ensures good contact during run Technical Spe
Front data ports–enable recording of instrument performance if required Gel Plate Size . .
by GLP Gel Size . . . . . .
Entire protocol can be seen on screen–easy to read and edit Maximum Power
Large display–provides real-time graphical results Maximum Tempe
Stores multiple protocols each with multiple steps–flexible programming Humidity . . . . .
for precise results Unit Dimensions
SE900 Rating . .
Technical Specifications Isoelectric Focusing (IEF) Separation of Buffer Pump . . .
Voltage . . . . . . . . . . . . . . . . . . . . 12,000 V
Mouse Liver using Hoefer IEF100 and 4-7 Weight . . . . . . .
Resolution . . . . . . . . . . . . . . . . . 1 µA IPG Strips Safety Certificatio
Image courtesy of Professor Peter James and Dr. Sofia Waldermarson,
Sample Cup Capacity . . . . . . . . . 240 µl Department of Immunotechnology, Lund University, Sweden
Unit Dimensions (w x h x d) . . . . 38 x 19 x 27 cm
Reference Paper Available Ordering Info
Data Connectivity . . . . . . . . . . . Ethernet / RS232
Cat # Des
Current . . . . . . . . . . . . . . . . . . . . 10 mA (999 µA/strip) “A Device for Isoelectric Focusing of IPG Gel Strips”
SE900-1.0 Larg
Platform Temperature . . . . . . . . . 15-25°C (Peltier Controlled) To receive your copy visit our website: SE900 Larg
Trays . . . . . . . . . . . . . . . . . . . . . . Running and Rehydration Tray www.hoeferinc.com
Complete Unit Inc
Weight . . . . . . . . . . . . . . . . . . . . 6 kg
• Separation Tank
Safety Certifications . . . . . . . . . . EN61010-1, UL61010-1,
• Lid with High Vo
CSA22.2 1010.1, CE
• Gel Rack
Ordering Information Basic Unit Include
Cat. # Description and hinged glass c
IEF100 Isoelectric Focusing Unit
Includes: 252 Wicks, 1 Running Tray, 1 Rehydration Tray,
1 Cleaning Brush, 1 Set of Forceps, and 10 Sets of Running Cups
(Each Set Containing 6 Sample Cups)

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 SE900 Large Format
Vertical Gel Electrophoresis Unit
For highly reproducible second-dimension
separations on up to six 28 cm SDS-PAGE gels

The SE900 was designed as a dedicated unit for second dimension

electrophoretic separation. It can accommodate up to six 28 cm SDS-PAGE gels
which mirrors the throughput of the IEF100 (6 IPG strips). If desired, the unit will
accommodate plates of similar size made by other manufacturers.

Features and Benefits

No upper buffer chamber–no gasket issues and no risk of buffer loss
Utilizes minimal parts–easy to use and reliable
External cooling and internal buffer circulation–ensures an even, constant,
and uniform temperature for reproducible results
Up to six 28 cm plates can be run at one time–perfect for high
throughput applications
Glass plate sandwiches are hinged cassettes–allows for rapid and easy
assembly before use
Built-in draining port–avoids lifting of a heavy buffer-filled tank
Assembly of the tank is easy–no clamps needed
Innovative design enables re-use of tank buffer–decreased buffer
consumption for reduced cost

Technical Specifications
Gel Plate Size . . . . . . . . . . . . . . . 28 x 21 cm (w x h)
Gel Size . . . . . . . . . . . . . . . . . . . 25 x 20 cm (w x h)
Maximum Power Settings . . . . . . 600 V, 1000 mA, 150 W
Maximum Temperature . . . . . . . 45°C
Humidity . . . . . . . . . . . . . . . . . . Up to 80%
Unit Dimensions (w x h x d) . . . . 43 x 43 x 20 cm
SE900 Rating . . . . . . . . . . . . . . . 100-240 V, 50-60 Hz, 2A
Buffer Pump . . . . . . . . . . . . . . . . 50,000 hour pump life
-7 Weight . . . . . . . . . . . . . . . . . . . . 13.6 kg
Safety Certifications . . . . . . . . . . EN61010-1, UL61010-1,
son, CSA22.2 1010.1, CE

Ordering Information
Cat # Description
trips” Corynebacterium Glutamicumcell
SE900-1.0 Large Format Vertical Electrophoresis Unit, Complete
SE900 Large Format Vertical Electrophoresis Unit, Basic lysates separated using the Hoefer
Complete Unit Includes: 2-D System and 4-7 IPG Strips
• Separation Tank • Multiple Gel Caster Image courtesy of Professor Angelika Görg, Technical
University of Munich, Germany.
• Lid with High Voltage Leads • Hinged Glass Cassettes–6 pcs
• Gel Rack
Basic Unit Includes: The same as above without the multiple gel caster
and hinged glass cassettes.

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 IPG BlueStrips
Precast immobilized pH gradient IPG gel strips eliminate
gradient drift and batch variability problems
IPG BlueStrips are low percentage, ultra thin, immobilized pH gradient gels cast
onto a plastic backing support. They are used for the isoelectric focusing of
protein samples and provide high resolution protein separations. The strips
can be used for rehydration or cup loading of proteins and can be used in all
commercially available isoelectric focusing devices including the IEF100. The
strips are available in five pH ranges and three strip lengths.
Technical Specifications
Packaging Size . . . . . . . . . . . . . . 12 IPG BlueStrips
Gel Size . . . . . . . . . . . . . . . . . . . Length: 7 cm, 18 cm, 24 cm
Width: 3 mm, gel thickness 0.5 mm
pH-Gradients . . . . . . . . . . . . . . . 3-10; 3-10 NL*; 4-7, 6-10, 3-6
Storage Temperature . . . . . . . . . . -20°C
*NL = non-linear, in the pH range 5-7 the gradient has enhanced resolution
Cat. # Description Size
IPG4300101 IPG BlueStrips 3-10/7 cm pk/12 strips
IPG4300201 IPG BlueStrips 3-10 NL/7 cm pk/12 strips
IPG4300301 IPG BlueStrips 4-7/7 cm pk/12 strips
IPG4300401 IPG BlueStrips 6-10/7 cm pk/12 strips
IPG4300501 IPG BlueStrips 3-6/7 cm pk/12 strips
IPG4301101 IPG BlueStrips 3-10/18 cm pk/12 strips
IPG4301201 IPG BlueStrips 3-10 NL/18 cm pk/12 strips
IPG4301301 IPG BlueStrips 4-7/18 cm pk/12 strips
IPG4301401 IPG BlueStrips 6-10/18 cm pk/12 strips
IPG4301501 IPG BlueStrips 3-6/18 cm pk/12 strips
IPG4302101 IPG BlueStrips 3-10/24 cm pk/12 strips
IPG4302201 IPG BlueStrips 3-10 NL/24 cm pk/12 strips
IPG4302301 IPG BlueStrips 4-7/24 cm pk/12 strips
IPG4302401 IPG BlueStrips 6-10/24 cm pk/12 strips
IPG4302501 IPG BlueStrips 3-6/24 cm pk/12 strips

Carrier Ampholytes
Ensure reliable and reproducible separation of protein
bands by IEF
• High resolution due to multimeric composition
• Fast staining and destaining times
• Clear background associated with very low unspecific binding of dyes and stains
• High solubility in trichloroacetic acid (fast removal of ampholytes during fixation)
ll
• Virtually no interaction with metal ions
r
• SERVALYT carrier ampholytes are produced according to the highest quality
standards and are routinely tested for performance
Cat. # Description Size
SER4294401 pH 3-6 10 ml
SER4294404 pH 3-6 2 ml
SER4294001 pH 3-10 10 ml
SER4294004 pH 3-10 2 ml
SER4294801 pH 4-7 10 ml
SER4294804 pH 4-7 2 ml
SER4291301 pH 6-10 10 ml
SER4291304 pH 6-10 2 ml

www.hoeferinc.com
10250 Hoefer 6pg broch_2:Layout 1 9/22/09 4:25 PM Page 1

SE600X Chroma™ Deluxe Complete 2-D

Dual Cooled Vertical Unit Integrated 2-D ele
Ergonomic design offers easier handling
and sample introduction
Mini 2-D Electroph
Technical Specifications
Glass Plate Size (w x h) . . . . . . . 18 x 16 cm or 18 x 8 cm Ordering Information
Spacers . . . . . . . . . . . . . . . . . . . 0.75 mm, 1.0 mm, 1.5 mm Cat. # D
Maximum Power Settings . . . . . . 1000 V, 500 mA, 50 W 526003 M
Maximum Temperature . . . . . . . 45°C P
Indoor Use . . . . . . . . . . . . . . . . . 4-40°C IEF100 I
Humidity . . . . . . . . . . . . . . . . . . Up to 80% SE260-10A-.75 M
Unit Dimensions (w x h x d) . . . . 32 x 29 x 14 cm SE2619T-2-1.0 T
Safety Certifications . . . . . . . . . . EN61010-1, UL61010-1, CSA C22.2 1010.1, CE PS300B 3

Cat. # Description
SE600X-15-1.5 Deluxe Dual Cooled Vertical Electrophoresis Unit, Complete Standard 2-D Elect
Includes: 1 Unit, Dual Gel Caster, 6 Glass Plates, 2-15 well 1.5 mm combs, and 4-1.5 mm spacers
Cat. # D

SE260 Mighty Small II Deluxe Mini

560003 S
P
IEF100 I
Vertical Electrophoresis Unit SE600X-15-1.5 D
PS300B 3
Combines the advantages of small format and efficient
cooling for rapid screening
Technical Specifications Large 2-D Electrop
Glass Plate Size (w x h) . . . . . . . 10 x 10.5 cm or 10 x 8 cm Cat. # D
Spacers . . . . . . . . . . . . . . . . . . . 0.75 mm, 1.0 mm, 1.5 mm E2DCLASSIC L
Maximum Power Settings . . . . . 500 V, 500 mA, 12 W P
Maximum Temperature . . . . . . . 45°C IEF100 I
Indoor Use . . . . . . . . . . . . . . . . 4-40°C SE900 L
Humidity . . . . . . . . . . . . . . . . . Up to 80%
Unit Dimensions (w x h x d) . . . 16.5 x 18 x 16 cm
Safety Certifications . . . . . . . . . EN61010-1, UL61010A-1, CSA C22.2 1010.1, CE
Large 2-D Electrop
Cat. # Description Cat. # D
SE260-10A-.75 Mighty Small II Deluxe Mini E2DELITE L
Vertical Electrophoresis Unit, Complete P
IEF100 I
Includes: 1 Unit, Dual Gel Caster, 10 Glass Plates, 5 Notched Alumina Plates,
2-10 well 0.75 mm combs, and 4-0.75 mm spacers SE900 L
SE9102-1-1.0 L
SE915 L
PS300B 300 Volt Power Supply
The versatile PS300B is designed for most Large 2-D Electrop
electrophoresis techniques
Cat. # D
Convenient and Simple 590003 L
The small footprint, large handle, and simple operation makes the PS300B easy to set P
up and use in your lab. With the large, easy to read LED, simply set the constant IEF100 I
parameter, set the timer (if desired), and push start. SE900 L
• Constant voltage or constant current mode with automatic crossover SE9102-1-1.0 L
• 10-300 V, 4-500 mA, 90 W SE915 L
PS265-115V 6
Cat. # Description
PS300B 300 Volt Power Supply, Universal VAC
For Large 2-D Electrophoresis Syste

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Complete 2-D Electrophoresis Packages
Integrated 2-D electrophoresis systems and combination packages

Mini 2-D Electrophoresis System

Ordering Information 2-D
Cat. # Description
526003 Mini 2-D Combination Package
Package Includes:
IEF100 Isoelectric Focusing Unit
SE260-10A-.75 Mighty Small II Mini Deluxe Vertical Unit
SE2619T-2-1.0 T-Spacers, 1.0 mm Thick (qty. 2)
PS300B 300 V, 500 mA, 90 W Power Supply

Standard 2-D Electrophoresis System

Cat. # Description
560003 Standard 2-D Combination Package
Package Includes:
IEF100 Isoelectric Focusing Unit
SE600X-15-1.5 Deluxe Dual Cooled Vertical Unit
PS300B 300 V, 500 mA, 90 W Power Supply

Large 2-D Electrophoresis System (Classic)

Cat. # Description
E2DCLASSIC Large 2-D Combination Package
Package Includes:
IEF100 Isoelectric Focusing Unit
SE900 Large Format Vertical Electrophoresis Unit

Large 2-D Electrophoresis System (Elite)

Cat. # Description
E2DELITE Large 2-D Combination Package
Package Includes:
IEF100 Isoelectric Focusing Unit
SE900 Large Format Vertical Electrophoresis Unit
SE9102-1-1.0 Large Hinged Glass Cassettes (qty. 6)
SE915 Large Multiple Gel Caster

Large 2-D Electrophoresis System w/Power Supply Mini Vertical U

Cat. # Description
590003 Large 2-D Combination Package w/P.S. 84 October Hill Road
Package Includes: Holliston, MA 01746-1388 USA
IEF100 Isoelectric Focusing Unit Toll Free: (800) 227-4750
SE900 Large Format Vertical Electrophoresis Unit Tel: +1 (508) 893-8999
Fax: +1 (508) 429-5732
SE9102-1-1.0 Large Hinged Glass Cassettes (qty. 6)
E-mail: [email protected]
SE915 Large Multiple Gel Caster Web: www.hoeferinc.com
PS265-115V 600 V, 500 mA, 150 W Power Supply
©Copyright, Hoefer®, Inc. 2009 Printed in USA
Hoefer® is a registered trademark of Hoefer, Inc.
For Large 2-D Electrophoresis System with 230 VAC power supply, order part number 590003-230V Chroma™ is a trademark of Hoefer, Inc.
SERVALYT™ is a trademark of SERVA