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Shimadzu IRSolution QuantGuide

This document provides instructions for creating a multiple point calibration curve using FTIR spectroscopy for quantitative analysis. It describes steps to acquire standard spectra, select the quantification mode, load standard files and enter concentration values to generate a calibration curve. It also outlines analyzing unknown samples by running them, selecting the calibration curve and obtaining analyzed results. The key point is samples and standards must have equivalent pathlengths for accurate quantitative analysis.

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67 views

Shimadzu IRSolution QuantGuide

This document provides instructions for creating a multiple point calibration curve using FTIR spectroscopy for quantitative analysis. It describes steps to acquire standard spectra, select the quantification mode, load standard files and enter concentration values to generate a calibration curve. It also outlines analyzing unknown samples by running them, selecting the calibration curve and obtaining analyzed results. The key point is samples and standards must have equivalent pathlengths for accurate quantitative analysis.

Uploaded by

chemchemha
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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IRsolution Quantitative Analysis:

Multiple Point Calibration Curve


FTIR is commonly used as a qualitative tool for identifying compounds. FTIR
spectroscopy can also be a useful tool for quantitative analysis. The critical point to bear
in mind is that the pathlength of the measured samples must be equivalent to that used for
measuring the standards.

Creating a Calibration Curve


1.) Acquire and save each standard s spectrum.
2.) Select the [Quant] tab

3.) With the [Quant] tab selected, next select <Multi


Point> as your method.

4.) With the [Quant] window open, you can now select the files you wish to use as
your standards.
5.) The standard spectra files will then be automatically loaded onto your window.

As seen above. You can now enter concentration values into your worksheet table.
6.) To select
calibration
method, on the
<Multi Point>
tab, under the
<Evaluation>
section decide
on Peak Area or
Height. Then
enter the values
of the desired
range.

7.) Click <Calibrate>, this will graph the points and the calibration curve.
8.) To review a report of the Multi point calibration curve, click on the <MP Result>
tab.
9.) You will now need to save the calibration data. To do this, go to [File] and scroll
down to <Save As>. Choose the appropriate directory. The file extension is
*.smf.

Analyzing Unknown Samples


10.).Run unknown samples to obtain spectra, and save.
11.) Return to the Quant Mode and go to <MP Analyze>.
12.) Select the unknown file you wish to analyze by double clicking on it, and it will
appear on the <MP Analyze> data set, automatically displaying the graph and data
analyzed by the calibration curve.
This document was created with Win2PDF available at http://www.daneprairie.com.
The unregistered version of Win2PDF is for evaluation or non-commercial use only.

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