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Prepared By:-: Priyanka Yadav M.Sc. Life Sciences Ist Sem

The document summarizes the cytoskeleton of eukaryotic cells. It discusses the three main types of cytoskeletal filaments - microtubules, microfilaments, and intermediate filaments. It describes their structures, functions in providing cell shape, strength, and motility, and differences between the three filament types.

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225 views37 pages

Prepared By:-: Priyanka Yadav M.Sc. Life Sciences Ist Sem

The document summarizes the cytoskeleton of eukaryotic cells. It discusses the three main types of cytoskeletal filaments - microtubules, microfilaments, and intermediate filaments. It describes their structures, functions in providing cell shape, strength, and motility, and differences between the three filament types.

Uploaded by

learta
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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PREPARED BY :-

PRIYANKA YADAV
M.Sc. Life sciences Ist
sem
CONTENTS :
INTRODUCTION
 HISTORY TIMELINE
STRUCTURE
FUNCTIONS
CYTOPLASMIC FILAMENTS
INTERMEDIATE FILAMENTS
MICROTUBULES
MICROFILAMENTS
DIFFERENCES
SUMMARY
TYPICAL EUKARYOTIC CELL
INTRODUCTION
This is a scan of the first electron micrograph taken intact of eukaryotic cell.
CYTOSKELETON
The cytoskeleton is the structure consisting of fibrous proteins that occur
in the cytoplasm and maintain the shape of the cell.
History Timeline
• 1942 Discovery of actomyosin
• 1954 Sliding filament model for muscle contraction
• 1965 Dynein, the first microtubule-dependent motor
• 1968–1978 Identification of intermediate filaments
• 1972–1977 Actomyosin contractile ring in cytokinesis
• 1973 Isolation of the first non-conventional myosin
• 1984 Microtubule dynamic instability
• 1989 & 1995 g-Tubulin and microtubule-organizing centres
• 1973 Isolation of the first non-conventional myosin
• 1984 Microtubule dynamic instability
• 1989 & 1995 g-Tubulin and microtubule-organizing centres
STRUCTURE
• Network of filamentous proteins
• filaments formed from a few proteins
• monomer protein forms polymer filaments

• located in nucleus and cytoplasmic compartments


• not within organelles

• location based upon cellular function


• named on basis of physical size
FUNCTIONS :
functions based upon the filaments physical properties
integral strength
cell shape
motility
1. inside the cell
2. whole cell
3. motor proteins associated with 2 filament systems
signal transduction

Note - the Extracellular Matrix has a similar structural role


outside of the cell.
Cytoskeletal filaments:
1. Microfilaments
2. Microtubules
3. Intermediate
filaments
INTERMEDIATE FILAMENTS
different cell types, different intermediate filaments
all eukaryotes nuclear cytoskeleton the same
resist stresses applied externally to the cell cytoplasm
10-nanometer diameter
cross-linking proteins allow interactions with other cytoskeletal
networks
intermediate filament associated proteins (IFAPs)
coordinate interactions between intermediate filaments
and other cytoskeletal elements and organelles,
human disorders
mutations weaken structural framework
increase the risk of cell rupture
Some functions of Intermediate Filaments :
 Intermediate filaments provide mechanical strength and
resistance to shear stress.
 There are several types of intermediate filaments, each
constructed from one or more proteins characteristic of it.
MICROTUBULES
25 nm diameter, 14 nm internal channel tubulin cytoplasmic
All cells contain
Same core structure
Same motors Dynein (-) and Kinesin (+)
Different associated proteins
Dynamic
Continuous remodelling
Movement
Intracellular > cellular
Cell division mitotic spindle
Specialized structures
centrosome,Spindle pole
good Cell processes - cilia (9+2)
MICROTUBULE STRUCTURE
STRUCTURE OF MICROTUBULE
If the rate of polymerization is If the rate of GTP hydrolyses is
faster than the rate of GTP faster then the rate of polymerization
hydrolysis, the microtubule will , the microtubule will disassemble
grow (It contains a GTP cap) (GTP cap is lost)
Some functions of Microtubules :
 Microtubules participate in a wide variety of cell activities.
 Most involve motion that is provided by protein “motors”
that use ATP.
 They determine the positions of membrane-enclosed
organelles and direct intracellular transport.
 The migration of chromosomes during mitosis and meiosis
takes place on microtubules that make up the spindle fibers.
MICROFILAMENTS
Twisted chain 7 nm diameter
most abundant protein in cells (5% of all cell protein)
Motility
Adhesion
Actin binding proteins
myosin motors
Muscle actins
CELL CRAWLING DEPENDS ON ACTIN
ACTIN BINDING PROTEINS
FORMINS – promotes the addition of G actin to the end of an unbranched filament
MUSCLE CONTRACTION
Actin filaments are anchored to the Z-disc at their + ends
The actin filaments face each other in the sarcomere
Upon contraction, the myosin heads moves towards the + end
Pulls the actin filaments towards each other.
Some functions of actin filaments are:
- To provide mechanical strength to the cell by forming a
band under the plasma membrane
- Link transmembrane proteins to cytoplasmic proteins
- Form contractile ring during cytokinesis in animal cells
- Cytoplasmic streaming
- Generate locomotion in cells such as white blood cells and
amoeba
- Interact with myosin to provide force of muscular
contraction
DIFFERENCES
SUMMARY
• The cytoskeleton organizes the structures and activities of the cell.
• The cytoskeleton interacts with motor proteins.
• The cytoskeleton also plays a major role in cell motility.
• There are three main types of fibers in the cytoskeleton: microtubules,
microfilaments, and intermediate filaments.
• Microtubules, the thickest fibers, are hollow rods about 25 microns in
diameter.
• Microfilaments, the thinnest class of the cytoskeletal fibers, are solid rods of
the globular protein actin.
• Microfilaments are designed to resist tension.
• Intermediate filaments, intermediate in size at 7nanometers, are specialized
for bearing tension.
• Intermediate filaments are more permanent fixtures of the cytoskeleton than
are the other two classes.

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