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Appendix B Using A Microscope PDF

The document describes the key parts and functions of a compound microscope. It explains that a compound microscope contains eyepiece and objective lenses that work together to magnify objects. The eyepiece lens magnifies 10x while the low-power and high-power objective lenses magnify 10x and 40x respectively. To calculate total magnification, multiply the eyepiece magnification by the objective lens magnification in use. Proper use and focusing of the microscope is also outlined.

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Jae Garciso
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315 views2 pages

Appendix B Using A Microscope PDF

The document describes the key parts and functions of a compound microscope. It explains that a compound microscope contains eyepiece and objective lenses that work together to magnify objects. The eyepiece lens magnifies 10x while the low-power and high-power objective lenses magnify 10x and 40x respectively. To calculate total magnification, multiply the eyepiece magnification by the objective lens magnification in use. Proper use and focusing of the microscope is also outlined.

Uploaded by

Jae Garciso
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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SX05_BkP_Apdx_B_Lab_new.

qxd 5/10/04 3:05 PM Page 150

The microscope is an essential tool in the study of life high-power objective lenses. The low-power objective
science. It allows you to see things that are too small lens usually magnifies 10 . The high-power objective
to be seen with the unaided eye. lens usually magnifies 40 .
You will probably use a compound microscope like To calculate the total magnification with which you
the one you see here. The compound microscope has are viewing an object, multiply the magnification of
more than one lens that magnifies the object you view. the eyepiece lens by the magnification of the objective
Typically, a compound microscope has one lens in the lens you are using. For example, the eyepiece’s
eyepiece, the part you look through. The eyepiece lens magnification of 10  multiplied by the low-power
usually magnifies 10 . Any object you view through this objective’s magnification of 10  equals a total
lens would appear 10 times larger than it is. magnification of 100 .
The compound microscope may contain one or two Use the photo of the compound microscope to
other lenses called objective lenses. If there are two become familiar with the parts of the microscope and
objective lenses, they are called the low-power and their functions.

The Parts of a Compound Microscope

Eyepiece
Contains a lens that magnifies about 10 

Body tube
Separates the eyepiece lens
from the objective lens

Coarse adjustment knob


Moves the body tube for focusing Nosepiece
with the low-power objective lens Holds the low-power and high-power
objective lenses; allows the lenses to
Fine adjustment knob rotate for viewing
Moves the body tube for focusing
with the high-power objective lens
Arm High-power objective lens
Supports the body tube Magnifies about 40 

Stage
Supports the slide being used Low-power objective lens
Magnifies about 10 
Stage clip
Diaphragm
Holds the slide in place
Controls the amount of light
passing through the opening
of the stage
Base
Supports the Mirror
microscope Reflects light upward
through the diaphragm

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SX05_BkP_Apdx_B_Lab_new.qxd 5/10/04 3:07 PM Page 151

Using the Microscope Making a Wet-Mount Slide


Use the following procedures when you are working with Use the following procedures to make a wet-mount slide
a microscope. of a specimen.
1. To carry the microscope, grasp the microscope’s 1. Obtain a clean microscope slide and a coverslip.
arm with one hand. Place your other hand under CAUTION: Glass slides and coverslips are fragile.
the base. 2. Place the specimen on the slide. The specimen
2. Place the microscope on a table with the arm must be thin enough for light to pass through it.
toward you. 3. Using a plastic dropper, place a drop of water on
3. Turn the coarse adjustment knob to raise the the specimen.
body tube. 4. Gently place one edge of the coverslip against the
4. Revolve the nosepiece until the low-power slide so that it touches the edge of the water drop
objective lens clicks into place. at a 45° angle. Slowly lower the coverslip over the
5. Adjust the diaphragm. While looking through the specimen. If air bubbles are trapped beneath the
eyepiece, also adjust the mirror until you see a coverslip, tap the coverslip gently with the eraser
bright white circle of light. CAUTION: Never use end of a pencil.
direct sunlight as a light source. 5. Remove any excess water at the edge of the
6. Place a slide on the stage. Center the specimen coverslip with a paper towel.
over the opening on the stage. Use the stage clips
to hold the slide in place. CAUTION: Glass slides
are fragile.
7. Look at the stage from the side. Carefully turn the
coarse adjustment knob to lower the body tube
until the low-power objective almost touches the
slide.
8. Looking through the eyepiece, very slowly turn
the coarse adjustment knob until the specimen
comes into focus.
9. To switch to the high-power objective lens, look
at the microscope from the side. Carefully revolve
the nosepiece until the high-power objective lens
clicks into place. Make sure the lens does not hit
the slide.
10. Looking through the eyepiece, turn the fine
adjustment knob until the specimen comes into
focus.

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