Department of Microbiology,St.

Ann’s College for Women, Mehdipatnam, Hyderabad

(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC

Department of Microbiology
Course Structure & Credits

Paper and code HPW Credits

Year Semester Title
Theory Practical
Introductory 4+3 4+1=5
I MIC101 MIC 111
Microbiology-I
I
Introductory 4+3 4+1=5
II MIC202 MIC 212
Microbiology-II
III MIC303 MIC 313 Microbial Physiology 4+3 4+1=5

COC Medical Coding 2 1

II
IV MIC404 MIC 414 Microbial Genetics 4+3 4+1=5

COC Medical Coding 2 1

Immunology & 3+3 3+1=4
MIC505 MIC 515 Chemotherapy

A- Environmental 3+3 3+1=4

Microbiology &
V
Sustainable Agriculture
MIC506 MIC 516
B- Food & Dairy
Microbiology
III
Medical Billing & 2 1
IDC Microbiology for Health
and Wealth
Industrial Microbiology
MIC607 MIC 617
&Microbial Technology 3+3 3+1=4
A. Medical Microbiology
VI
B. Clinical & Diagnostic 3+3 3+1=4
MIC608 MIC 618
Microbiology

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC

SEMESTER-V

COURSE TITLE: IMMUNOLOGY AND CHEMOTHERAPY (Theory)

Course Code: MIC 505
No of Periods: 45

UNIT – I Fundamentals and anatomy of Immune system 12 hours

 Introduction to Immunology
 Types of immunity – Innate and Acquired; Active and Passive
 Cells of immune system- B &T Lymphocytes, Null cells, Monocytes, Macrophages,
Neutrophils, Basophils & Eosinophils.
 Primary and Secondary organs of immune system- Thymus, Bursa of Fabricius,
Bone Marrow, Spleen, Lymphnodes and Mucus Associated Lymphoid Tissue (MALT).
 Immune response- Humoral and Cell-mediated immunity.

UNIT – II Antigens and Antibodies 11 hours

 Antigens – types, chemical nature, antigenic determinants, haptens, factors affecting
antigenicity.
 Antibodies – basic structure, types, properties and functions of immunoglobulins.
 Monoclonal antibodies – production and applications.
 Complement system - properties, components, pathways, functions

UNIT-III Antigen- Antibody reactions 11 hours

 Types of antigen-antibody reactions – agglutination, precipitation, complement fixation and

neutralization
 Labeled antibody based techniques – ELISA, RIA and Immunofluroscence.
 Types of hypersensitivity – Immediate and Delayed.

UNIT-IV Chemotherapy 11 hours

 Introduction to Chemotherapy
 Cell wall Inhibitor -Mode of Action of Penicillin & Clinical Use
 Protein Synthesis Inhibitor-Mode of Action of Streptomycin & Clinical Use
 Sulfonamides-Mode of Action & Clinical Use
 Tests for antimicrobial susceptibility – MIC, Kirby- Bauer assay, Phenol Coefficient
 A brief account on antimicrobial resistance

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC

References:
1. Sudha Gangal. Shubhangi Sontakke. Text book of Basic and Clinical Immunology,
Universitie Press.
2. Tizard, I.R. (1995). Immunology : An Introduction, WB Saunders, Philadelphia, USA.
3. Riott, I.M. (1998). Essentials of Immunology, ELBS and Black Well Scientific Publishers,
England.
4. Goldsby, Kindt, T.J. and Osborne, B.A. (2004). Kuby Immunology, 6th Edition,
W.H.Freeman and Company, New York.
5. Lydyard, P.M., Whelan, A. and Fanger, M.W. (2000). Instant Notes in Immunology, Viva
Books Pvt. Ltd., New Delhi.
6. Chakraborty, B. (1998). A Text Book of Microbiology, New Central Book Agency (P) Ltd,
Calcutta, India.
7. Ananthanarayana, R. and Panicker, C.K.S. (2000). Text Book of Microbiology, 6th Edition,
Oriental Longman Publications, USA.
8. Annadurai, B. (2008). A Textbook of Immunology and Immunotechnology. S. Chand & Co.
Ltd., New Delhi.
9. Dey, N., T.K. and Sinha, D. (1999). Medical Bacteriology Including Medical Mycology and
AIDS. New Central Book Agency (P) Ltd. Calcutta, India.
10. Shetty, N. (1994). Imuunology – Introductory Textbook. New Age International Pvt. Ltd.,
New Delhi.
11. Reddy, S.R. and Reddy, K.R. (2006). A Text Book of Microbiology - Immunology and
Medical Microbiology, Himalaya Publishing House, Mumbai.
12. Gupte, S. (1995). Short Text Book of Medical Microbiology, 8th Edition, Jaypee
Brothers Medical Publishers (P) Ltd, New Delhi
13. Gale(1991),Molecular basis of Antibiotic action

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC

COURSE TITLE: IMMUNOLOGY AND CHEMOTHERAPY (Practicals)

Course Code: MIC 515

1.Determination of blood groups and Rh typing

2.Estimation of blood haemoglobin by Sahli’s method
3.Total Count of RBC
4.Total Count of WBC
5. Differential Count by Leishman’s Staining.
6. Demonstration of ELISA
7. Tests for disinfectant (Phenol coefficient).
8. Antibiotic sensitivity testing – MIC and Disc diffusion method.

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC
References:

1.Talwar, G.P. and Gupta, S.K. (1992). A Hand Book of Practical and Clinical Immunology. CBS
Publications, New Delhi.
2.Baren, E.J. (1994). Bailey and Scott’s Diagnostic Microbiology, 9th Edition, Mosby Publishers.
3.Dubey, R.C. and Maheswari, D.K. (2002). Practical Microbiology, S. Chand & Co., New
Delhi.
4. Samuel, K.M. (Ed.) (1989). Notes on Clinical Lab Techniques, M.K.G. Iyyer & Son
Publishers,Chennai.
5.Wadher, B.J. and Reddy, G.L.B. (1995). Manual of Diagnostic Microbiology, Himalaya
Publishing House, Mumbai.
6.Dey, N.C., Dey, T.K., Dey, M. and Sinha, D. (1998). Practical Microbiology,Protozoology
and Parasitology. New Central Book Agency (P) Ltd. Calcutta.
7.Mukherjee, K.L. (1996). Medical Laboratory Technology. Vol II. Tata Mc GrawHill
Publishing Co. Ltd., New Delhi.

8.Gopal Reddy, M., Reddy, M.N., Saigopal, DVR and Mallaiah, K.V. (2007). Laboratory
Experiments in Microbiology, 2nd edition. Himalaya Publishing House, Mumbai
9. Fiachbach F (1996) Laboratory Diagnostic tests. 5th edition Lipincott

10.Collee G., Dugid J.P. (1989) Mackie and McCartney Practical Medical Microbiology .
Churchill Livingstone

11.Godkar P.b.and Godkar D. P. (2003) Textbook of Medical Laboratory Technology. 2 nd edition .

Bhalani publications, Mumbai.

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC

SEMESTER-V

COURSE TITLE: ENVIRONMENTAL MICROBIOLOGY & SUSTAINABLE

AGRICULTURE (Theory)
Course Code: MIC 506A
No of Periods: 45

UNIT - I Water & Air Microbiology 11 hours

 Microbes of water ( E. coli ,Clostridium perfringens and Streptococcus faecalis as

indicators of water pollution.) and their significance
 Microbiological testing of potable water - MPN index, membrane filtration
 Water purification in municipal water supply- Physical and Chemical treatment
 Waste water treatment (primary, secondary and tertiary).
 Microbes of air and air sampling methods

UNIT - II Soil Microbiology 11 hours

 Soil- definition, types, physical and chemical characters, soil profile
 Microbes of soil and their significance
 Microbial Interactions-Mutualism,Commensalism,Antagonism, Parasitism
 Biogeochemical Cycles(Carbon,Nitrogen,Sulphur,Phosphorus) & Role of microorganisms
in nutrient cycling

UNIT-III Sustainable Agriculture 12 hours

 Rhizosphere and phyllosphere
 Plant growth-promoting microorganisms -Mycorrhizae, Rhizobia, Azospirillum,
Azotobacter, Cyanobacteria, Frankia and phosphate-solubilizing microorganisms.
 Outlines of biological nitrogen fixation (symbiotic, non-symbiotic).
 Biofertilizers - Rhizobium & Cyanobacteria
 Biopesticides – Bacillus thuringiensis,Nuclear polyhedrosis virus (NPV)

UNIT-IV Biodegradation and Bioremediation 11 hours

 Biodegradation of cellulose, hemicellulose, lignin
 Microbial remediation of Xenobiotics–oil spills, organophosphorous pesticides
 Solid waste disposal-sanitary landfills, composting
 Biogas- Biohydrogen, Biomethane

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC

References:

1.Subba Rao, N.S. (1993). Biofertilizers in Agriculture and Forestry, 3rd Edition Oxford & IBH
Publishing Co. Pvt. Ltd., New Delhi.

2. Rangaswami, G. and Bhagyaraj, D.J. (2001). Agricultural Microbiology, 2nd Edition, Prentice
Hall of India, New Delhi.

3.Atlas, R.M. and Bartha, R. (1998). Microbial Ecology - Fundamentals and Applications,
Addison Wesley Longman, Inc., USA

4.Paul, E.A. and Clark, F.E. (1989). Soil Microbiology and Biochemistry, Academic Press,

5.Alexander, M. (1985). Introduction to Soil Microbiology, 3rd Edition. Wiley Eastern Ltd., New
Delhi.

6.Subba Rao, N.S. (1999). Soil Microorganisms and Plant Growth. Oxford & IBH Publishing Co.
Pvt. Ltd., New Delhi.

7.Coyne Mark (2006) . Soil Microbiology. Delmar publishers.

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC

COURSE TITLE: ENVIRONMENTAL MICROOBIOLOGY & SUSTAINABLE

AGRICULTURE (Practicals)
Course Code:MIC 516A

1. Microbial testing of water by coliform test (presumptive,confirmed and completed test)

2. Determination of biochemical oxygen demand (BOD) of polluted water
3. Isolation of microorganisms of air by Petri plate exposure method.
4. Study of Soil Microbiota-Isolation, Identification and enumeration of major groups of
microorganisms from rhizosphere and non rhizosphere.
5. Isolation of antagonistic microorganisms by crowded plate technique.
6. Isolation of Rhizobium from legume root nodules
7. Isolation of Azotobacter/Phosphate solubulizers from soil
8. Model for compost making and study of microbial communities.

References:

1. Aneja, K.R. (2001). Experiments in Microbiology, Plant pathology, Tissue culture

and Mushroom Production Technology, 3rd Edition, New Age International (P)
Ltd., New Delhi.
2. Dubey, R.C. and Maheswari, D.K. (2002). Practical Microbiology, S. Chand & Co.,
New Delhi.
3. Burns, R.G. and Slater, J.H. (1982). Experimental Microbiology and Ecology.
Blackwell Scientific Publications, USA.
4. Peppler, I.L. and Gerba, C.P. (2004). Environmental Microbiology – A Laboratory
Manual. Academic Press. New York.
5. Gupte, S. (1995). Practical Microbiology. Jaypee Brothers Medical Publishers Pvt.
Ltd.
6. Kannan, N. (2003). Hand Book of Laboratory Culture Medias, Reagents, Stains and
Buffers. Panima Publishing Co., New Delhi.
7. Gopal Reddy, M., Reddy, M.N., Saigopal, DVR and Mallaiah, K.V. (2007). Laboratory
Experiments in Microbiology, 2nd edition. Himalaya Publishing House, Mumbai.

8. Reddy, S.M. and Reddy, S.R. (1998). Microbiology – Practical Manual, 3rd Edition, Sri
Padmavathi Publications, Hyderabad

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC

SEMESTER-V
COURSE TITLE: FOOD AND DAIRY MICROBIOLOGY (Theory)
Course Code: MIC 506B
No of Periods: 45

UNIT - I Basics of Food Microbiology 11 hours

 Food and microorganisms - Food as a substrate for microorganisms and sources of
contamination of food
 Introduction to Food Spoilage and spoilage of fruits & vegetables, meat and meat products
and canned foods
 Microbiological examination of fresh and canned foods

UNIT-II Food poisoning and Food preservation 12 hours

 Food intoxication -Staphylococcal poisoning, Botulism
 Food borne infections – Salmonellosis and Shiegellosis
 Food preservation - Principles of food preservation, Methods of food preservation- high
temperature, canning, freezing, dehydration, chemical preservatives and radiation

UNIT-III Applied Food Microbiology 11 hours

 Microbiological production of fermented foods-Health aspects
Bread
Cheese
Yoghurt
 Microorganisms as food: Single cell proteins and edible mushrooms
 Concept of Probiotics

UNIT-IV Dairy Microbiology 11 hours

 Microorganisms in milk and sources of microbial contamination of milk
 Microbiological analysis of milk:
a. Rapid tests - DMC
b. Standard plate count
c. Dye reduction test - MBRT, Resazurin test
 Biochemical activities of milk
 Recent advances in dairy industry

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC

References:

1.Adams MR and Moss MO. (1995). Food Microbiology. 4th edition, New Age International (P)
Limited Publishers, New Delhi, India.

2. Banwart JM. (1987). Basic Food Microbiology. 1st edition. CBS Publishers and Distributors,
Delhi, India.

3. Davidson PM and Brannen AL. (1993). Antimicrobials in Foods. Marcel Dekker, New York.

4. Dillion VM and Board RG. (1996). Natural Antimicrobial Systems and Food Preservation. CAB
International, Wallingford, Oxon.

5. Frazier WC and Westhoff DC. (1992). Food Microbiology. 3rd edition. Tata McGraw-Hill
Publishing Company Ltd, New Delhi, India.

6. Gould GW. (1995). New Methods of Food Preservation. Blackie Academic and Professional,
London.

7. Jay JM, Loessner MJ and Golden DA. (2005). Modern Food Microbiology. 7th edition, CBS
Publishers and Distributors, Delhi, India.

8. Lund BM, Baird Parker AC, and Gould GW. (2000). The Microbiological Safety and Quality of
Foods. Vol. 1-2, ASPEN Publication, Gaithersberg, MD.

9. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An Introduction. 9th edition.
Pearson Education.

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC

COURSE TITLE: FOOD AND DAIRY MICROBIOLOGY (Practicals)

Course Code:MIC 516B

1. Isolation and identification of microbes from spoilt fruits and vegetables

2. Isolation and identification of microbes from fermented foods
3. Bacterial examination of milk by- SPC, DMC, MBRT
4. Estimation of Lactose in milk
5. Estimation of Lactic acid in milk
6. Observation of food-borne pathogens - Clostridium, Staphylococcus and Salmonella
7. Production of yoghurt and bread

References:

1.Gopal Reddy, M., Reddy, M.N., Saigopal, DVR and Mallaiah, K.V. (2007). Laboratory
Experiments in Microbiology, 2nd edition. Himalaya Publishing House, Mumbai.

2. Reddy, S.M. and Reddy, S.R. (1998). Microbiology – Practical Manual, 3rd Edition, Sri
Padmavathi Publications, Hyderabad

3.Aneja, K.R. (2001). Experiments in Microbiology, Plant pathology, Tissue culture and
Mushroom Production Technology, 3rd Edition, New Age International (P) Ltd., New Delhi.

4.Dubey, R.C. and Maheswari, D.K. (2002). Practical Microbiology, S. Chand & Co., New Delhi.

5.Cappuccino, J.G. and Sherman, N. (2005). Microbiology – A Laboratory Manual. 7th Edition.
Pearson

6.USA Alcamo, I.E. (2001). Laboratory Fundamentals of Microbiology. Jones and Bartlett
Publishers, USA.

7.Benson, J.H. (2005). Microbiological Applications: Laboratory Manual in General

Microbiology. 7th Edition, McGraw Hill Publications, New York.

8.Subhashini D. V. (2013) Laboratory Manual for Basic and Applied Microbiology. Kalyani
Publishers.
Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K

 Department of Microbiology,St. Ann’s College for Women, Mehdipatnam, Hyderabad
(Autonomous) , Osmania University
NAAC Reaccredited with ‘A+’ Grade(3rd cycle), College with Potential for Excellence by UGC
9.National Dairy Research Institute , Bangalore ( ICAR ) (1982) . Manual in Dairy Microbiology.

10.Manoharachary C (2006) Handbook of soil Fungi. I.K. international.

Names & Signatures

1. Dr.B.Bhima 2. Dr.D.Madhusudhan Reddy 3. Dr.Nivedita Sahu 4. Dr.S.Sreedevi

5.Dr.Y.L.Paruchuri 6. Ms.Radhika Tamane 7.Mrs.Jyothi.Ch 8.Mrs.Krishnapriya.K