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Bacterial Isolation Case Study Analysis

An inmate with a self-inflicted open wound was brought to the ER. A culture and sensitivity test was performed using the appropriate transport media and prescribed media. The next day, rapid test results found the bacteria was catalase positive and oxidase negative. Further biochemical testing and antibiotic susceptibility testing was then performed, with results to be recorded the following day to identify the isolated bacteria.

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Lueshen Wellington
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71 views4 pages

Bacterial Isolation Case Study Analysis

An inmate with a self-inflicted open wound was brought to the ER. A culture and sensitivity test was performed using the appropriate transport media and prescribed media. The next day, rapid test results found the bacteria was catalase positive and oxidase negative. Further biochemical testing and antibiotic susceptibility testing was then performed, with results to be recorded the following day to identify the isolated bacteria.

Uploaded by

Lueshen Wellington
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Case Study # 1

A prison inmate is brought into the ER room with an open wound and is known for self-
infecting. A routine culture and sensitivity (C & S) test is ordered. The appropriate
transport media is used for specimen collection and for inoculation of prescribed media.

Day 1 Culture Readout: Below are the results of the routine culture after overnight in a CO2
incubator at 35 ⁰C .

Gram stain of bacterial colony


Organism isolated on BAP Organism isolated on MAC (Oil – 1000x total magnification)

Rapid Test Results: Catalase positive and oxidase negative


Isolated colonies are used to set up biochemical testing and perform antibiotic susceptibility
testing for readout the next day.
Day 2:
Record the results
for the following
biochemical tests
(e.g. positive or
negative) in the
chart provided.

E
SIM MR VP Citrate TSI
What bacteria was isolated?

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