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Case Study # 3

A 32-year-old male motorcycle accident victim on a ventilator for several weeks had a respiratory culture done. A moderate growth of an organism was isolated from his mucus that was catalase positive and oxidase negative on Gram stain. Biochemical testing results identified Pseudomonas aeruginosa.

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Lueshen Wellington
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44 views

Case Study # 3

A 32-year-old male motorcycle accident victim on a ventilator for several weeks had a respiratory culture done. A moderate growth of an organism was isolated from his mucus that was catalase positive and oxidase negative on Gram stain. Biochemical testing results identified Pseudomonas aeruginosa.

Uploaded by

Lueshen Wellington
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Case Study # 3

A 32-year male motorcycle accident victim has


been on a respiratory ventilator for over several
weeks. He is suctioned and mucous collected for
a respiratory routine culture & sensitivity test.

Day 1 Culture Readout: A small amount of normal respiratory flora was isolated on
blood agar after overnight in a CO2 incubator at 35 ⁰C. However, a moderate growth
of an organism was isolated on MAC.

Rapid Test Results: Catalase


positive and oxidase negative

Isolated colonies are used to


Gram stain of bacterial colony
set up biochemical testing for Bacterial colonies
from MAC
read out the next day. isolated on MAC
(Oil – 1000x total magnification)
Day 2:
Record the results
for the following
biochemical tests
(e.g. positive or
negative) in the
chart provided.

A B C D E
SIM MR VP Citrate TSI
What bacteria was isolated?

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