OPERATOR MANUAL

BSA3000 Semi biochemistry analyzer

SFRI sarl - Berganton - 33127 Saint Jean d’Illac – France

Tel : +33 (0) 556 688 050 Fax : +33 (0) 556 217 903
e-mail : [email protected]
OPERATOR MANUAL

Contents
Statement 3
Chapter 1. General 5
Chapter 2. Specifications 6
Chapter 3. Requirements 8
Chapter 4. System structure 9
Chapter 5. System Menu 10
Chapter 6. Operation 16
Chapter 7. Maintenance 18
Chapter 8 Troubleshooting 19
Chapter 9 Abbreviations 21
Safety symbols 22

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Statement
SFRI sarl owns all rights to this unpublished work and intends to maintain this work as confidential.
SFRI sarl may also seek to maintain this work as an unpublished copyright. This publication is to be
used solely for the purposes of reference, operation, maintenance, or repair of SFRI sarl equipment.
No part of this can be disseminated for other purposes.

In the event of inadvertent or deliberate publication, SFRI sarl intends to enforce its rights to this
work under copyright laws as a published work. Those having access to this work may not copy, use,
or disclose the information in this work unless expressly authorized by SFRI sarl to do so.

All information contained in this publication is believed to be correct. SFRI sarl shall not be liable for
errors contained herein nor for incidental or consequential damages in connection with the furnishing,
performance, or use of this material. This publication may refer to information and protected by
copyrights or patents and does not convey any license under the patent rights of SFRI sarl, nor the
rights of others. SFRI sarl does not assume any liability arising out of any infringements of patents or
other rights of third parties.

Content of this manual is subject to changes without prior notice.

Responsibility on the manufacturer party

SFRI sarl is responsible for safety, reliability and performance of this equipment only in the condition
that:
• all installation, expansion, change, modification and repair of this equipment are conducted by
SFRI sarl qualified personnel;
• applied electrical appliance is in compliance with relevant National Standards;
• the Analyzer is operated under strict observance of this manual.

Warning
For continued safe use of this equipment, it is necessary that the listed instructions are followed.
However, instructions listed in this manual in no way supersede established medical practices
concerning patient care.
z This equipment is intended for use only by medical professionals in health care institutions.
z To avoid electrical shock, you shall not open any cover by yourself. Service must be carried
out by qualified personnel.
z It is dangerous to expose electrical contact or applicant coupler to normal saline, other liquid
or conductive adhesive. Electrical contact and coupler such as cable connector, power supply
must be kept clean and dry. Once being polluted by liquid, they must be thoroughly dried. If
to further remove the pollution, please contact your biomedical department or SFRI sarl.

It is important for the hospital or organization that employs this equipment to carry out a reasonable

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maintenance schedule. Neglect of this may result in machine breakdown or injury of human health.

Upon request, SFRI sarl may provide, with compensation, necessary circuit diagrams, calibration
illustration list and other information to help qualified technician to maintain and repair some parts,
which SFRI sarl may define as user serviceable.

Warranty

THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED,
INCLUDING WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE.

Workmanship & Materials

SFRI sarl guarantees new equipment other than accessories to be free from defects in workmanship
and materials for a period of one year from date of shipment under normal use and service. SFRI
sarl's obligation under this warranty is limited to repairing, at SFRI sarl’s option, any part which upon
SFRI sarl's examination proves defective.

Exemptions

SFRI sarl's obligation or liability under this warranty does not include any transportation or other
charges or liability for direct, indirect or consequential damages or delay resulting from the improper
use or application of the product or the substitution upon it of parts or accessories not approved by
SFRI sarl or repaired by anyone other than a SFRI sarl authorized representative.

This warranty shall not extend to any instrument which has been subjected to misuse, negligence or
accident; any instrument from which SFRI sarl's original serial number tag or product identification
markings have been altered or removed, or any product of any other manufacturer.

Safety, Reliability and Performance

SFRI sarl is not responsible for the effects on safety, reliability and performance of the ESR3000 if:
■ Assembly operations, extensions, re-adjusts, modifications or repairs are carried out by persons
other than those authorized by SFRI sarl.
■ Personal unauthorized by SFRI sarl repairs or modifies the instrument.

Company Contact
Address: SFRI sarl
Berganton
33127 SAINT JEAN D’ILLAC
FRANCE
Phone: +33 (0) 556 688 050
Fax: +33 (0) 556 217 903
e-mail [email protected]
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Chapter 1. General
The chemistry analyzer is an instrument utilizing optical, mechanical and computer
technologies. It is an important laboratory instrument running most routine clinical chemistry
tests. Chemistry analyzers can be divided into two categories – the fully automatic analyzer
and the semi automatic analyzer. The fully automatic analyzer completes most of the routine
operations automatically, such as sample & reagent pipe ting, mixing, heating, analyzing,
calculating, printing and rinsing. It meets the demand of hospitals with large sample volume
and diverse test items.

The semi automatic analyzer automates certain operations (such as heating, analyzing,
calculating and printing), while reaction solution has to be prepared manually. This type of
instrument is more suitable to small laboratories and hospitals with smaller sample volume.

The BSA-3000 is a semi-auto chemistry analyzer with high precision and high reliability. It features:
o Analysis methods: end point, kinetic, fixed time and absorbance
o Linear and non-linear calibration
o Touch screen, large LCD display
o Anti-vibration and anti-disturbance optical system ensures highly accurate and
reliable results
o Measurement precision of 0.0001ABS and calculation precision of 0.00001ABS
o Reaction curve displayed
o Lamp saving feature largely prolongs lamp life
o Comprehensive quality control program, Levey-Jenning graphics included
o Audible / visual alarm available
o Built-in high-speed thermal printer
o Reagent open.

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Chapter 2. Specifications
LIGHT SOURCE
z Quartz-halogen lamp 12V/20W

WAVELENGTH RANGE
z Automatic by 8 positions filter-wheel
z 7 standard filters: 340, 405, 492, 510, 546, 578, 630nm
z 1 position free for optional filter
z Half Bandwidth < 8nm
z Stray light: < 1.0 % @ 340 nm

PHOTOMETRIC RANGE
z 0.0000 to 3.0000 ABS
z Resolution: 0.0001 ABS
z Drift: 0.002 A/hour

FLOWCELL
z Stainless steel body with quartz window
z Measuring volume 32 uL
z Aspiration volume programmable: 200 – 2000 uL
z Optical path 10mm

ANALYSIS METHOD
z End point, with or without reagent blank
z End point, with sample blank and with or without reagent blank
z Kinetic, with or without linearity check
z Fixed time, with or without reagent blank
z Absorbance
z Turbidimetry
z Linear and non-linear standardization

PARAMETER SETTINGS
z Method
z Wavelength Mono or Bichromatic readings
z Temperature
z Reagent blank y/n
z Sample blank y/n
z Delay time
z Measuring time
z Reaction type
z Absorbance limit
z Aspiration volume

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z Standards
z Linearity check
z Unit for results

MEMORY
z 3000 results

TEMPERATURE CONTROL
z By means of Peltier elements
z 25, 30, 37 °C optional
z Precision: +/- 0.1°C

INPUT
z Touch screen

DISPLAY
z Large LCD display

PRINTER
z Built-in thermal printer

INTERFACE
z RS232 serial port

OPREATING ENVIRONMENT
z Temperature: 15 – 30 °C
z Humidity: 20 – 80 %

POWER REQUIREMENT
z AC 220V +/- 10%, 50/60Hz
z AC 110V +/- 10%, 50/60Hz

DIMENSION
z 42cm x 38cm x 17cm

WEIGHT
z 7.5 kg

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Chapter 3. Requirements

3.1 Operating Environment:

1. The instrument should be installed on a stable and solid platform that is free of

mechanical vibration and away from vibration source.

2. The environment should be as free as possible from dust, corrosive gas, loud noises and

electrical interference.

3. Avoid placing the instrument in direct sun or in front of a source of heat or draft.

4. Ambient temperature: 15~30°C, humidity: 20~80%.

3.2 Power Requirements:

1. The input voltage should be 220/110 VAC +/- 10%, 50/60Hz.

2. Power supply and grounding should be correctly connected.

Note: Verify that the input voltage is compatible with the instrument and the proper type of fuse

is installed.

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Chapter 4. System structure

4.1 Front View

Printer

Touch screen

Start key

Sample probe

4.2 Rear View

Peristaltic RS232 Fan Power Power Grounding

Pump Plug Switch Pole

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Chapter 5. System menu

The main menu of the BSA-3000 includes six sub-menus: Measure, Quality Control, Setup,
Program, Print Report and Work list, as the figure below shows.

Main Menu

Measure Quality Control

Setup Program

Print Report Work list

5.1 Measure
5.1.1 Select test: the semi-automatic chemistry analyzer can conduct only one type of test
at a time. The operation is: touch Measure, then select the test to be conducted.
5.1.2 Measure:
For the end point, results should be taken when the reaction is over. Add the sample or
the standard to the reaction tube, and then add the reagent to the tube. After
incubation, take the colorimetric results on the instrument.
For the kinetic or fixed time, the results should be taken within the steady rate range of
the reaction. Add sample to the tubes, then add reagents and take results immediately.
For the end point, there are two ways to obtain the result. One is to check the A-C curve,
and the other is to calculate with the following equation:

C = (Sample ABS－Reagent Blank ABS－Sample Blank ABS)×Factor

Standard concentration
Factor =

（Standard ABS—Reagent blank ABS）

The equation of the fixed time is almost same as that of the end point. The difference is
the sample ABS, reagent blank ABS, calibrator ABS should be changed to the ABS
variation ( A) within the measuring time.

Calculation of the kinetic method is based on ABS variation rate ( Ａ／min),

Ｃ＝ Ａ／min×Factor

ＴＶ×１０００

Factor ＝
ＳＶ× d ×ε

TV: total reaction volume, SV: sample volume, d: optical path,ε: Moore constant.

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5.2 Quality Control

QC graphics, settings, deleting data and printing data are included.
5.2.1 QC graphics: established from QC data automatically.
5.2.2 Settings: set mean value and SD value.
5.2.3 delete data: Select “Delete Last Month” and only the QC data of the last month will be
deleted. Select “Delete All Data” and all QC data of the past 31 days will be deleted.
5.2.4 Print: Touch Print to print out detailed QC information.

5.3 Setup
5.3.1 Date and Time: system date and time. The date format is: Year-Month-Day. The time
format is: Hour:Minute:Second. For example, the time is 18:35 on December 23, 2002,

then the operation is: touch Date and Time , touch 2, 0, 0, 2 ， YES ；touch

1, 2, YES ；touch 2, 3, YES ；touch 1, 8, YES ； touch 3 , 5

, YES .
5.3.2 Volume Cal.: To calibrate aspiration volume. After a certain period, the aspirating
force of the pump tube may change, which will cause the discrepancy between the set
aspiration volume and the actual aspiration volume. Therefore, aspiration volume
should be calibrated regularly, usually once per week. The operation is: touch
Volume Cal. and then present 1 ml distilled water to the sample probe. Press the
Start key to active the aspiration. When the water is all aspirated into the instrument,
release the Start key to stop the aspiration, then the new calibration value is displayed.
Usually this step is to be repeated several times until the difference between the new
calibration value and current value is less than 10%.
5.3.3 Feed paper:
The operation is: touch Feed paper and the printer will run a while to load the paper.
5.3.4 Send Data: to transmit the sample result data and QC data to PC. The operation is:
touch Send Data . When the transmission is done, the system will return to the previous
menu.

5.3.5 Light source: touch Light Source，select Turn on or Turn off , and the corresponding

image will be displayed.

5.3.6 Rinse: the flow cell should be rinsed after each type of test. First rinse with distilled
water, then rinse with the recommended detergent, and finally rinse with distilled
water again. The rinsing volume should be no less than 2 ml. The operation is: touch

Rinse , press the Start key, and rinse the flow cell with distilled water 一 detergent —

distilled water.
5.3.7 Result review: two ways to review the results, by the test or by the sample. The
operation is: touch Review by Test , select the test name, then all the results for this
test will be printed out. Or, touch Review by ID , enter the sample’s ID number, then

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all the results for this sample will be printed out.

5.3.8 Zero drift check: Fill the flow cell with distilled water, continuously read the
absorbance at 340 nm wavelength for 10 minutes. The difference between the
maximum and minimum absorbance value should be no greater than 0.006 ABS. The
operation is: touch Zero Drift , present the distilled water to sample probe, then press
the Start key. The zero drift check will start after the aspiration. The maximum,
minimum and current absorbance values (ABS) are all displayed on the screen. After 10
minutes, the system will display the message “Zero drift check passed” or “Zero drift
check failed”.
5.3.9 Filter check: Check the position and absorbance of each filter. For the new instrument,
all the filters’ absorbance should be less than 0.200, except for the free position (F8).

The operation is: touch Filter Check，present the distilled water to the sample probe,

and press the Start key. After the aspiration is done, the filters will be checked and the
results will be displayed on the screen.

5.4 Program
5.4.1 New Test: 26 tests are pre-programmed and stored in the instrument. The parameters
of these tests can be changed by the user, while the test number and test name are
fixed and cannot be changed or deleted. Besides the pre-programmed tests, the user
can define new tests as necessary. Up to 6 characters can be entered for the test name.
The operation is: touch Program , and the following menu will be displayed:

Program

Edit Test

New Test

Delete Test

Touch New Test , and the following menu will be displayed:

New Test
15 LDH 16 HBDH
17 CK 18 CK-MB
19 CO2 20 BUN
21 Cr 22 Ca
23 p 24 MG
25 UA 26 AMS
27 28
Select a new number, then the following menu will be displayed.
Test Name ＿＿＿＿

1 ABC .1 2DEF

3 GHI 4JKL

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5 MNO .2 6PQR

7STU 8VWX
9YZ 0
. NEXT
YES CLR
For example, assuming ABS is to be entered, the operation is: touch 1ABC twice,
then “A” is displayed. Touch NEXT, touch 1ABC three times, then “AB” is displayed.
Touch NEXT, touch 7STU twice, then “ABS” is displayed. Touch YES and return
to the previous menu.
Caution
Changing the test name is prohibited if the result of this test is still present.
5.4.2 Edit Test: Different tests require different parameters. Test parameters include:
method, wave length, temperature, aspiration volume, reagent blank, delay time,
measuring time, reaction type, ABS limit, decimal, standard, factor, linear check, unit,
measure range and normal range. The following description takes glucose as the
example.
5.4.2.1 Method: the following methods can be selected: Absorbance, End Point, Fixed time,
and Kinetic. Select End Point for the glucose. The operation is: touch 09GLU ,
Method and select End Point .

5.4.2.2 Wavelength: seven wavelengths（340nm, 405nm, 492nm, 510nm, 546nm, 578nm,

630nm）can be selected. For the glucose the wavelength is 492nm.

The operation is: touch Wavelenght and select 492nm . Reselect Wavelenght
2 times a “2” appears on the right of wevelenght then :
If you want to work in Monochromatic mode select 492nm again
If you want to work in Bichromatic mode select the 2nd wavelenght
5.4.2.3 Temperature: 4 options available -- room temperature, 25 , 30 and 37 . Room
temperature is selected for GLU. The operation is: touch Temperature and
select Room Temp .
5.4.2.4 Reagent blank: Yes --with reagent blank, No -- without reagent blank. No reagent
blank for the glucose. The operation is: touch Reagent Blank and select No .
5.4.2.5 Delay time: For the Absorbance and End Point, the delay time is usually 1 second.
For the fixed time and kinetic, the delay time should be set according to the
reagent instruction sheet. The operation is: touch Delay time then enter the

required time．

5.4.2.6 Measuring time: For the absorbency and end point, the measuring time is usually 1
second. For the fixed time and kinetic, the time should be set according to the
reagent instruction sheet. The operation is: touch Measuring time then enter the

required time．

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Note:
The interval reading time for fixed time and kinetic method is 10 seconds.
5.4.2.7 Reaction type:
Increase: the absorbance value increases during the reaction.
Decrease: the absorbance value decreases during the reaction.
This parameter does not apply to the glucose. Skip this item.
5.4.2.8 ABS limit: The range is 0.0000-3.0000A. For the increase reaction, the absorbance of
the last reading point should not be greater than the limit. Otherwise, the result is
followed by a “*” . For the decrease reaction, the absorbance of the last reading
point should not be lower than the limit. Otherwise, the result is followed by a “*” .
This parameter does not apply to the Absorbance and End Point
5.4.2.9 Decimal: 0 for integer results, 1 for one decimal place, 2 for two decimal places, 3
for three decimal places. Select 2 for GLU. The operation is : touch Decimal and
select 2.
5.4.2.10 Factor: For the kinetic, the factor is entered according to the reagent instruction
sheet. For the end point and fixed time, the factor is calculated by measuring the
standard. For the absorbance, the factor is 1. For the glucose, the factor is
calculated by measuring the standard.
5.4.2.11 Linear check: If the measuring result is non-linear, NL will be displayed. There is no
linear check for the end point or the absorbance
5.4.2.12 Unit: seven result units can be selected: U/L, mmol/L, umol/L, mg/L, G/L, mg/dl
and ABS. For the glucose the unit is mmol/L. The operation is: touch Unit and
select mmol/L .
5.4.2.13 Aspiration volume: To set the volume of the reaction mixture to be aspirated into
the flow cell. Too small aspiration volume may cause high carryover. The
recommended volume is 500ul~1000ul. For example, assuming 1000ul is to be
entered, the operation is: touch Aspiration Vol , enter 1 0 0 0 ,
then touch YES .
5.4.2.14 Measure range: “$”is displayed in case the result exceeds the range. For the

glucose, the range is 0.00-2.22. The operation is: touch Measure Range H ，enter

2 . 2 2 YES , then touch Measure range L , enter 0 . 0 0

YES .
5.4.2.15 Normal range: Reference range for normal results. “H” or “L” is automatically
printed when the test result exceeds the range. The operation is similar as previous
section.
5.4.2.16 Standard point: Four calibration modes are available: 0, 1, multi-point linear and
multi-point non-linear. “0” means no standard and applies to the absorbance and
the Kinetic. “1” means only one standard and applies to the end point and the fixed
time. Up to 6 standards can be defined with the multi-point linear method or
multi-point non-linear method. This applies to the end point only. Select 1 for the
glucose. The operation is: touch STD Point and select 1.
5.4.2.17 Standard value: Enter the standard concentration accordingly. No standard value

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entered for 0 standard point. Enter one standard value for 1 standard point. Enter
2~6 standard values for multi-point settings.
Note: If less than 6 standards to be defined, then after entering all the standard
values, set the value of the next point to 0. For example, if 4 standards to be
defined totally, then set the value of the 5th point to 0. In this way the system can
decide the number of standards. If the standard value of the glucose is 5.55, the
operation is: touch STD 1 , enter 5 . 5 5 YES.
5.5 Print report
Touch Print Report then all the test results of current date will be printed out by the
sample ID.
5.6 Work list
5.6.1 Touch Worklist to input the work list at the main menu. Select the sample ID, then
select the tests to be conducted. The selected items will be displayed in reverse
video. Touch Exit after entering all the tests for this sample. Repeat this
operation for all the samples.
5.6.2 Check and confirm the work list. Touch Print by ID then all the selected test items
will be printed out by the sample ID. Check and correct the errors, then proceed to
next step.
5.6.3 Print a list by the test. Touch Print by Test then a list will be printed out by test
items. This helps the user to prepare the samples and reagents properly.
5.6.4 Sample measurement: At the main menu, touch Measure and all the tests will be
displayed. Each test to be conducted is preceded by a “ ”. Select the test to enter
the menu of the next level. After running zero, standardization and quality control,
touch Sample to measure the samples. (when “ ” is displayed inside ID: XXX ,
the sample ID will change automatically according to the work list, thus it can not be
changed by the user) Measure the samples in turn. The measuring results will be saved
to the system automatically. When all the samples for the current test item are
finished, “ ” will blink and disappear, then the user can change the sample ID
freely and measure the suspicious samples again. After that, rinse the flow cell with
distilled water and return to the main menu. Repeat this step for the other tests.
Proceed to next step when all the tests are finished.

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Chapter 6. Operation

6.1 Check before Power-On

The operating environment should be as free as possible from dust, corrosive gas,
mechanical vibrations, loud noises and electrical interference. Avoid placing the instrument
in direct sun or in front of a source of heat or draft. Power supply and grounding are
properly connected. The input voltage should be compatible with the instrument. Ambient
temperature and humidity is within the related requirements.

6.2 Power-On
Turn on the power. After initialization, the system will enter the main menu automatically.
Caution
Proceed to the following operations only after 10~15 minutes warm-up.

6.3 Calibrating the Aspiration Volume

Usually the aspiration volume is calibrated once a week. See Section 5.3.2 “Volume Cal” .

6.4 Checking Filter：

See Section 5.3.9 “Filter check”.

6.5 Setting Test Parameters

Different tests require different parameters. Set the parameters as instructed by Section
5.4.2 “Edit Test ”. The parameters are saved automatically.

6.6 Measure
The measuring procedure is explained as follows (take the glucose as the example):

6.6.1 At the main menu，touch Measure

6.6.2 Select test：touch 09GLU

6.6.3 Measure：

Before measuring, check the parameters that are displayed on the top of the screen, and
make sure they are correct.

6.6.3.1 Zero：touch zero and present the distilled water to the sample probe. Press the Start

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key to aspirate the distilled water. After zeroing, “ A: 0.000 ” will be displayed on the
screen.
Note: If the test parameter for “Reagent Blank” is “No”, the user should execute this step
with reagent instead of distilled water.

6.6.3.2 Reagent blank：touch RB and present the reagent to the sample probe. Press the Start

key to aspirate the reagent. After reading the absorbance of the reagent, the value will
be displayed on the screen.
Note: If the test parameter for “Reagent Blank” is “No”, RB will not be displayed.

6.6.3.3 Standard：touch Standard and present the standard-reagent mixture to the sample

probe. Press the Start key to aspirate the mixture. The absorbance reading of the
mixture and the factor are displayed. The factor is calculated and saved into the
instrument automatically.

6.6.3.4 Quality control：touch QC and present the control to the sample probe. Press the

Start key to aspirate the control. The absorbance reading and measuring result of the
control are displayed. The measuring result is saved into the QC file.

6.6.3.5 Sample：touch Sample and present the sample to the sample probe. Press the Start

key to aspirate the sample. The absorbance reading and measuring result of the sample
are displayed. The result is also printed out automatically. Repeat this step to measure
the samples in turn.

6.6.3.6 Change sample ID：press Next ID or ID XXX to change the sample ID.

Caution
Make sure the sample has the same ID for different tests.

6.7 Exit Test

Touch Exit to exit the menu.

6.8 Wash Flow Cell

See Section 5.3.6 “Rinse”.

6.9 Select Other Tests

Repeat step 6.6~6.8.

6.10 Print Test Results

When all the tests are done, select “Print Report ” to print all the results by sample ID.

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Caution
Before shutdown, make sure to wash the flow cell thoroughly and fill it with distilled water.

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Chapter 7. Maintenance
7.1 The instrument must be operated under the environmental requirements.

7.2 After turning on the power or the lamp, wait 15~20 minutes before starting the test. After
turning off the lamp, wait about 10 minutes for the lamp to cool down before turning it on
again.

7.3 Make sure the measurement is free of bubbles.

7.4 Wash the flow cell after finishing each kind of test.

7.5 Check the aspiration volume regularly. If necessary, replace the aged tube.

7.6 Fill the flow cell with distilled water before shutdown.

7.7 Handle and dispose of the waste according to acceptable laboratory, local state and national
standards.

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Chapter 8. Troubleshooting

8.1 Black Screen after Power-On

Cause:（1）poor connection of the power cable;（2）broken fuse.

Solution:（1）connect the power cable properly；（2）replace the fuse.

8.2 Abnormal Aspiration or No Aspiration

Cause:（1）aged peristaltic pump tube；（2）tubing system blocked；（3）tubing system leaking

or loose；（4）defective peristaltic pump motor.

Solution:（1）replace peristaltic pump tube；（2）eliminate blockage；（3）check the tubing

system, if necessary, change the aged tube. （4）replace peristaltic pump motor (5)

contact the manufacturer.

8.3 No Aspiration When Start key is Pressed

Cause: calibrated value for aspiration volume is lost.

Solution: Shut down and re-start the instrument. Enter the Setup \ Volume Cal menu to
re-calibrate the aspiration volume.

8.4 Abnormal Display of A-C curve

Cause: Improper value for standard point and concentration

Solution: set the correct parameters and re-create the curve.

8.5 Absorbance of the Filter is Too High

Cause:（1）bubbles in flow cell；（2）deviated optical parts; （3） aged lamp；（4）damaged

filter.

Solution:（1）wash flow cell with proper detergent；（2）adjust the optical components to

correct position; （3） replace the lamp；（4）replace the filter.

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8.6 Poor Repeatability of Test Results

Cause:（1）dirty flow cell subject to remaining bubbles；（2）tube blocked；（3）high carryover

（4）aged lamp；（5）poor internal ventilation or

due to inaccurate aspiration volume；

ambient temperature too high；（6）polluted reagent.

Solution:（1）wash flow cell with proper detergent and keep bubbles away；（2）replace the

tube；（3）re-calibrate aspiration volume or replace the peristaltic pump tube；（4）

replace the lamp； （5） operate the instrument under proper environment；（6）

use qualified reagent.

8.7 Results Too High or Too Low

Cause:（1） inaccurate standard volume；（2）poor standard；（3）wrong factor；（4）polluted

reagent ；（ 5 ） wrong temperature setting for kinetic method ；（ 6 ） wrong

sample-to-reagent ratio.

Solution:（1）add standard of correct volume；（2）use qualified standard；（3）correct the

factor；（4）use qualified reagent；（5）correct the temperature setting；(6) prepare

sample and reagent as instructed.

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Chapter 9. Abbreviations

No. Abbreviation Full Name

Alanine
01 ALT
aminotransferase
Aspartate
02 AST
aminotransferase
03 ALP Alkaline phosphatase
Gamma Glutamyl
04 GGT
transferease
05 T.BILI Total bilirubin
06 D.BILI Direct bilirubin
07 TP Total Protein
08 ALB Albumin
09 GLU Glucose
10 CHOL Cholesterol
11 TRIG Triglyceride
High density
12 HDL-CH
lipoprotein-cholesterol
13 APOA1 Apolipoprotein A1
14 APOB Apolipoprotein B
15 LDH Lactic dehydrogenase
16 HBDH α-hydroxybutyrate
17 CK Creatine kinase
18 CK-MB MB isoenzyme of CK

19 CO2 Carbon dioxide

20 BUN Blood urine nitrogen
21 Cr Creatinine
22 Ca2+ Calcium
23 P Phosphorus
24 Mg2+ Magnesium
25 UA Urine acid
26 AMS Amylase
27 K Kalium
28 Na Natrium
29 Cl Chlorine

BSA 3000 Operator Manual (V1.1) 22/22

OPERATOR MANUAL

Safety Symbols

Symbol Warning Condition Action

Biohazard: Consider all materials Wear standard laboratory attire,

(specimens, reagents, controls, glove and follow safe laboratory
calibrators, or components that procedures when handling any
contain or have contacted human material in the laboratory.
blood) as being potentially infectious.

Electrical Shock Hazard: Before continuing, unplug the

The electrical warning icon alerts BA-88 analyzer from the
users to the possibility of electrical electrical outlet.
shock in the described activity or at
the posted location.

Probe Hazard: The probe may Avoid any unnecessary contact

contain biohazard materials, including with the probe and probe area.
controls and calibrators.

NOTE: Indicate to the operator

information of importance in the
procedure to be performed. This
heading is also used to indicate
specific sample handling techniques
that are helpful in operating the
instrument.
CAUTION: Indicate to the
operator information of importance
that could result in damage to the
instrument or affect the test due to
improper operation if these
instructions are not followed.

WARNING: Indicate to the

operator information regarding
potential hazards that may cause
personal harm to the operator if these
instructions are not followed.

BSA 3000 Operator Manual (V1.1) 23/22