Keep Growing

BioFlo® 120 bioprocess control station

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»Performance meets value.«

The Eppendorf BioFlo 120 offers simplicity and ease of use, without sacrificing capability. No
matter if you are in an academic, governmental or industrial research setting, or working with
bacteria, yeast, fungi, mammalian, insect or plant cells, the BioFlo 120 is an attractive solution to
meet your needs–all at an affordable price.

Feature-packed and future-proof

>>Scale-up from 250 mL to 40 L on a wide variety of >>Universal connections for digital Mettler Toledo® ISM
autoclavable and Eppendorf BioBLU® Single-Use Vessels sensors or analog sensors offer unsurpassed flexibility
>>New Auto Culture modes offer process control for >>User-defined DO cascades offer process flexibility
microbial and cell culture applications at the touch of a >>Automatic gas mixing algorithms for simplified control
button >>View your entire process with expanded trend screen
>>Ready for process. Unbox and install in minutes >>Access your data from anywhere with Eppendorf SCADA
>>Save critical lab space with a minimal footprint platforms, IP network, and remote monitoring capabilities
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Connection for interchangeable

direct- and magnetic-drive motors; Easy-to-read 7” integrated
magnetic drive capable of clockwise and touchscreen monitor
counterclockwise rotation for simplified
impeller selection

Three user-defined
analog input /output All vessel
connections. Select connections
between 0-5V, 0-10V, located on the
and 4-20 mA depending side–set up and
on the device ready to go in
minutes

High-precision thermal
mass flow controller
(TMFC) or rotameter
for gas flow control.
Standard automatic IP21 rated enclosure
mixing of four for protection of
independent sparge gas sensitive electronics
supplies and cleanability.

Water recirculation module Three front-mounted fixed-speed pumps

provides precise temperature with industry-standard easy load pump
control and exhaust heads for convenient liquid addition/
condensing removal
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BioBLU® single-use advantage Sophisticated software

>>The rigid-walled design advantage: >>Eppendorf Bioprocess Control Software offers real time,
>>Sets up in minutes, no inflation necessary local process control with integrated touchscreen
>>No more tears, pits, or folds as seen in bag installations >>Optional Eppendorf SCADA platforms (BioCommand®,
>>Consistent leachables and extractables profile with DASware®) provide high level process control capabilities,
single-layer molded polymer vs. multi-layer bag design and secure database management
>>Scale from 250 mL to 40 L with BioBLU Single-Use Vessels >>IP networking provides remote access through PC or
>>BioBLU f vessels designed for high density fermentation mobile device
>>Eppendorf exclusive BioBLU 5p with Fibra-Cel® Disks for
continuous and perfusion processes

Scalable solutions Unlimited applications

>>Perfectly suited for research and development >>Grow any cell type you can think of: Microbial, insect,
>>Scale-up and scale-down modeling plant, fungal, mammalian, and stem cells
>>Growth of seed to pilot-scale cultures in a working volume >>Unlimited process flexibility: Batch, fed-batch, continuous,
range of 250 mL to 40 L or perfusion; supports high-density, micro-aerobic, and
>>Blends into the Eppendorf bioreactor and fermentor anaerobic fermentation, secreted products production, and
portfolio covering working volumes of 65 mL to 2,400 L, process development for cell and gene therapies
for comprehensive scalability

Find more detailed information, including video presentations, an online configurator and a 360°
product show on our website. Visit www.eppendorf.com/BioFlo120 or easily scan the QR code
beside.
 Liquid Handling
Cell Handling
Sample Handling

To make your job in the lab easier and more efficient – with this goal in mind
we are developing products and solutions in the areas of Liquid Handling,
Cell Handling, and Sample Handling. Visit the Eppendorf Handling Solutions
online sphere and dive into the area of your choice, learn new things, and have
fun as well: www.eppendorf.com/handling-solutions

Liquid Handling Cell Handling Sample Handling

In 1961, Eppendorf launched For handling cells, in addition Sample Handling encompasses
the first piston-stroke pipette. to manipulators and injectors, many different work processes
Today, our broad product incubators and consumables for and steps: centrifugation,
offerings in Liquid Handling cultivation as well as complete heating, freezing, mixing,
range from manual pipettes to bioreactor systems for cell culture amplification, and analysis of
electronic pipettes, dispensers applications are also available. samples. Eppendorf offers the
and burettes to automated Corresponding detection systems devices and consumables needed
pipetting systems. are offered for subsequent analysis. for each of these steps.

Eppendorf Easypet® 3 New Brunswick™ S41i Centrifuge 5920 R

Experience a new dimension of electronic The only CO2 incubator with an Eppendorf Experience extraordinary high capacity
pipetting with complete speed control and shaker inside. in a very compact and ergonomic product
the utmost precision. design.
>>Precise control of temperature, shaking
>>Intuitive and convenient speed adjustment speed and CO2 for stable culture conditions >>Swing-bucket rotors and adapters
simply done with the tips of your fingers >>Easy-to-clean chamber design and accomodate tubes and bottles from 0.2 mL
>>Lightweight, well-balanced and ergonomic 120°C disinfection cycle saves time and to 1,000 mL
design that allows for fatigue-free pipetting effectively eliminates contamination >>Fixed-angle rotors and plate rotor options

epMotion® 5075m Concerned about

Bacteria in
The most flexible member of our family of
automated liquid handling systems. Cell Culture?
>>Mixing, temperature control and magnetic
Find useful tips on
bead separation abilities
>>Optical sensor verifies labware, tips and www.eppendorf.com/cellexperts
volumes before the run
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Vessel Specifications
Autoclavable vessels
Vessel 1L 2L 5L 10 L
Total volume 1.3 L 3.0 L 7.5 L 14.0 L
Working volume 0.4 – 1.0 L 0.8 – 2.2 L 2.0 – 5.6 L 4.0 – 10.5 L
Vessel type Water-jacketed or heat-blanketed
Material Borosilicate glass, 316L stainless steel
Impellers
Direct drive or magnetic drive Rushton-type, pitched blade, marine or spin filter
Autoclave dimensions
Heat-blanketed
Outer diameter (OD) 20.3 cm 20.3 cm 29.8 cm 29.8 cm
8.0 in 8.0 in 11.7 in 11.7 in
Height (without exhaust filter) 54.0 cm 54.0 cm 61.0 cm 68.0 cm
21.3 in 21.3 in 24.0 in 26.9 in
Water-jacketed
Outer diameter (OD) 24.1 cm 24.1 cm 29.8 cm 29.8 cm
9.5 in 9.5 in 11.7 in 11.7 in
Height (without exhaust filter) 48.9 cm 56.5 cm 64.8 cm 76.8 cm
19.3 in 22.3 in 25.5 in 30.3 in
Number of head plate ports
6 mm 1 6 7 7
12 mm 9 7 8 8
19 mm 0 0 1 1
Total 10 13 16 16
Recommended sensor lengths (mm)
Sensor
pH (analog)1 200 225 325 425
pH/redox (digital)1 225 225 325 425
DO (analog)1 160 220 320 420
DO (digital/optical)1 220 220 320 420
Redox (analog)1 200 325 325 425
CO2 (digital)1 220 320 320 320

Single-use vessels
Vessel BioBLU 1c/f BioBLU 3c/f BioBLU 5c BioBLU 5p BioBLU 14c BioBLU 50c
Total volume 1.8 L 5L 5L 5L 14 L 50 L
Working volume 0.25 – 1.25 L 2 1.25 – 3.75 L 1.25 – 3.75 L 3.75 L 3.5 – 10.5 L 18 – 40 L
Vessel type Rigid-walled, stirred-tank
Impellers
Magnetic drive BioBLU c: pitched blade/BioBLU p: packed-bed/BioBLU f: Rushton-type
Recommended sensor lengths (mm)
pH (EC)3 220 225 225 100 425 625
DO3 220 225 225 120 355 526
Redox3 220 225 225 120 425 625
CO23 220 220 220 120 320 N/A

1
Installation may require compression fitting for optimal fit and depth (M1273-5040), 2 x included with Vessel Connection Kit.
2
BioBLU 1c: 425 mL minimal working volume when used with vessel stand and heat blanket
3
Installation may require compression fitting for optimal fit and depth (1386010200).

Specifications subject to change.

 BioFlo 120 Specifications
Control Station
Dimensions (W x D x H) 24.7 x 55.9 x 62.9 cm (9.7 x 22 x 24.8 in)
Net weight 14.8 kg (32.7 lb)
Touchscreen 7 in projected capacitive touchscreen
Communication 2 x USB (software updates, serial communication)
Ethernet (SCADA, IP Network)
Utility Connection Requirement
Electrical IEC-C14 (with regional plug types) 100 – 120/208 – 240 (± 10 %) V, 50/60 Hz, 10 A, Single Phase
Water Quick-connect 10 psig (0.69 barg)
Gas supply (air, O2, N2, CO2) Push-connect fittings accept 1/4 in Autoclavable Single-use
tubing or hose barb fitting 10 psig (0.69 barg) 6 psig (0.44 barg)
Exhaust 0.5 psig (0.035 barg)
Operating conditions 10 – 30°C, up to 80 % RH, non-condensing
Altitude limit 2000 m
Agitation
Direct drive 1 L: 25 – 1,500 rpm
3 L, 5 L, 10 L: 25 – 1,200 rpm
Magnetic drive (autoclavable vessels) 1 L, 3 L, or 5 L: 5 – 500 rpm
10 L: 5 – 150 rpm
Magnetic drive (single-use vessels) BioBLU 1f & 3f: 5 – 1,200 rpm
BioBLU 1c: 5 – 500 rpm
BioBLU 3c, 5c, 5p & 14c: 5 – 200 rpm
BioBLU 50c: 5 – 150 rpm
Temperature
Autoclavable 1, 2, 5 L: 8°C above coolant to 45°C above ambient (0°C – 70°C absolute)1
10 L: 8°C above coolant to 40°C above ambient (0°C – 65°C absolute)1
BioBLU Single-Use Vessels BioBLU c vessels: 5°C above ambient to 40°C1
BioBLU f vessels: 5°C above coolant to 45°C1
Sensor type Pt100
Gas supply
Sparge 1 TMFC (0.04 – 20 SLPM) or 1 rotameter (multiple options available); ring or microsparger
Sensors Communication Control range
pH Analog or digital Mettler Toledo ISM 2 – 12
DO Analog or digital Mettler Toledo ISM 0 – 200 % (air saturation)
Optical DO Digital Mettler Toledo ISM 0 – 200 % (air saturation)
Redox Analog or digital Mettler Toledo ISM (-)2000 mV – (+)2000 mV
CO2 Digital Mettler Toledo ISM 0 – 100 %
Pumps Pump head Fixed speed
Pumps 1, 2, & 3 Watson-Marlow 114DV 30 rpm (0 – 100 % duty cycle)
1
Requires 115/230 V line voltage. Specifications cannot be guaranteed with operation at alternative line voltages.

Specifications subject to change.

Your local distributor: www.eppendorf.com/contact

Eppendorf AG · 22331 Hamburg · Germany
[email protected] · www.eppendorf.com

www.eppendorf.com
Mettler Toledo® is a registered trademark of Mettler Toledo AG, Switzerland. Watson-Marlow® is a registered trademark of Watson-Marlow Limited, UK. Eppendorf®, the Eppendorf Brand
Design, BioBLU®, Easypet®, and epMotion® are registered trademarks of Eppendorf AG, Germany. New Brunswick™ is a trademark of Eppendorf AG, Germany. DASware® is a registered
trademark of DASGIP Information and Process Technology GmbH, Germany. BioFlo® and BioCommand® are registered trademarks of Eppendorf, Inc., USA.
All rights reserved, including graphics and images. Copyright © 2017 by Eppendorf AG.
Order No.: AN01 311 020/GB2/3T/0317/EBC/STEFF · Carbon neutrally printed in Germany.