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LIQUIXX
ALBUMTN ( ALB ) C(
BGG Dye Method, End Point @
INTENDEDUSE SPECIi'EN COLI.ECTION AND tllANDUNG
Diagnoslic reagent for quantilaUve i, vilio delerm naton of Use unheamolylic serum or plasma (EDTA, heparin)
Albumin in human serum and pasma. ll is recommonded lo follow NCCLS procedures (or similar
standardized condilions).
Stability ln 36rum: 1 month at 2-8'C
CLINICAL SIGNIFICANCE
1 week al 15-25'C
Albumin, a major plasma protein, is synthesized in lhe li\€r from
al least 3 monlhs at-20'C
amino acids which are absorbed from the ileum. lts funclion
Discard contaminaled specimens.
includes regulation and distribution of exlracell!lar fluid
transportalion of wrious hormones Mtamins and lrace metals
lncreased levels are observed in ASSAY PARAMETERS
Dehydration due to reduced plasma waler conlent. End Point
Slasis during \€nipuncture which causes flu d 1o escape into
Wa\€length 1 (nm ) 630
the extra scular comparlment.
Decreased levelsare ob6orved in Wa'Jelenoth 2 (nm l
Excessive protein loss (mainly albumin)- from kidney, skir or Sample Volume (ul) 5/10
ReaqenlVolume (ul) 500/1000
Decreased synthesis due to dietary, hepatic disease or lncubation Time (m in.) 1

lncrgased catabolism in fever unlrealed diabetes mellitls and lncLrbation Tem p. ('C) 37
3.5
Normal Hish (o/dl) 5.2
r, ETHODOLOGY Lineariiv Low (o/d ) 0.1
Doumas et alr. LinearilvHioh loldl) 6
Concentration of Standard (g/dl) 3.6
PRINCIPLE Bla nk with Reagent
Albumin binds with aromocresol green ( BCG )atpH 4.2causing Absorbance Lim il (lt4ax) 0.1
a shift in absorbance of the yellow BCG dye. The blue{reen Units
colour formed is propodional to the concenlration of albumin
present, when measured photom etrically between 580-630 nm Programme parameters for sp€cific clinical analysers are
with maxmum absorbance a1625 nm. available on requg5t

REAGENT COMPOSITION
ASSAY PROCEDURE
REAGENT 1: Albumin Reagent
Blank
0.08 mmoltr
Succi nate Bulfer ( pH 4.2 1 0.I at 25'C ) 50 mmol/L 1000 !l 1000 u 1000 !l
Sodium Azide I om/L 10u
Surfactanl 1o !
10 !l
Albumin Standard
lvix well, read lhe absorbance of standard and each test at
Albumin Standard I 3.6q/dt 630 nm ( 580630 nm ) againstreagent blank, afteroneminute
incubation at 37'C.
REAGENT PREPARATION
Reagents are liquid, ready to use. CALCULATION

STOMGE ANO STABILITY Absorbance of Test

Unopened reagent is stable till the epiry date stated on the
Albumin = X
Standard ( g/dl )
labelwhen stored below 30'C.
(g/dl ) Absorbance ol Standard

The Siandard is siable till the epiry date slaied on the label
when stored al 2-8'C. The reagent blank should have an
absorbance less lhan 0.10 at 630 nm against dislilled waler.

iI tlanutactured by : TRANSASIA Blo.MEDlcALs LTD., Nalagaft Road, vifiage tvtatpur, Baddi, Dist. sotan, (Hp) - 173205.
: €RBA diagnostics Mannh€im GmbH iklau3ti,69-73, D -68219, Mannhetm/c.m.ny
ln Technical Collaboration with
NOTES PERFORMA'{CE DATA
1. Gross lapaemia maycause falselyele\ated rcsulls.ln such Dala conlained within this section is
cases a sample blank may b€ setup asfollows:
performance on ERBA systems. Data
these lues.
laboralory maydiffer from
Pipette into tubes Limitorquantflcation: 0.1g/dl
A ank Sample Blank
Lineaiity: 6 g/dl
Saline 1000 ul 1000 !l Measuring range: 0.1 - 6 g/dl
SamD e 10 ul
PRECBION
Read the absorbance of sample blank against saline blank at
630 nm. Then subtract the absorbance ofthe sample blank from lntra-assay precision
SD (sidl) cv (%)
lhe absorbanco ofthe test.
2. Final absorbanc€ readings should be taken less than 90 3.303 0.017 0.51
seconds afler sample addilion. Sample 2 4.926 o.044 0.88
3. Ths reagent and sample volum€s may be attered
proportionally lo accomodate various analyzor lnteLassay precision
SD (g/dl) cv (%)
€quirements. Run lo run (n=20)
3.345 0.043 1.29
OUALITY CONTROL Sample 2 4.6'12 0 043 093
For quality control ERBA NORM and ERBA PATH are recom ms-
nded.
INTERFERENCES
Following substances do not interfere:
EXPECTED VALUES haemoglobin up to 10 q/|. b'lrrubin uo lo 40 ma/dl, tnglycerides
Serum: up Io 2000 mgTdl
0-4d 2-8 4.4gldl
4d- 14y 3.8- 5.4 g/dl WARNING AI{D PRECAUNO{S
M-1ay 3-2- 4-5 gldl For l, y,ifro diagnoslic use. To be handled by enttted and
professionally educatsd person.
20 60y Reagents of the kitare not classified as dangsrous but contain
60-90y 3.2 4-6gldl less than 0.1% sodium azid6 - classified as !€ry toxic and
dangBrcus substiance for th€ environmenl.
It is recomnbnded lrral 6ach laboratory vealfy lhls range or
dedyes reteaance intorval forthe population lt s€rv$.
WASTE i,lAT.IAGEi/l ENT
Please refer to local lsgal requirem6nls.
Coi'PARISON
A comparison bstween Liquin Albumin (y) and a comm€rciatty
a\ailable test (x) using 40 samples ga\i€ following resutts: REFEREI'ICES
y= 1-000x+0.065g/dl 1. Doumas B.I,Ar6Ms R-1., PintoP.C. in standad Mothods
r=0997 of Clinical Chemistry ( 1972 ) Vol. 7, p- 175.189, Academic
Pr6ss Chicago.

SYIrl4r.s: 2. ]]eE N. W. ( Ed. ), Todbook of Clinical Chsmistry W. B.

The following symbols are used in the tabotting of Saunders (1986 )p. 589.
ERBA Mannheim kils:

@ caaroe,u llo aa
\\
cE Ma'i - D€qc6hpv PACK PRESENTATIO{
wdr ln€ Dr6.ft 93,79Ec
Reagentl Albumin
@ e'r* coa"
@ rnvuo or"sno"t*
Co& Abumin Standard

8 [TJ|,""T[," *"o., ffi con",rr rn"r,*on ro, u""

120223 5 x 50ml 5 r 50rnl 1x 5ml

{ n"-*..*.,- Revision No.: 4- ALBUMIN

Date oflssue : 30/09/2015
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