TABLE OF CONTENT

CONTENT PAGE

1.0 ABSTRACT 1

2.0 INTRODUCTION 2

3.0 MATERIALS 3

4.0 METHOD 4

5.0 RESULTS 5

6.0 DISCUSSION 9

7.0 CONCLUSION 10

8.0 REFERENCE 11
1.0 ABSTRACT
This experiment was conducted to make students become familiar with compound and
stereoscopic microscope on its function, parts and how to use it. It is important to know about
instruments that we used in the lab. Next, this experiment will acquire the skill to calibrate
the microscope to measure the size of a sample using stage and ocular micrometer. Lastly, a
prepared slide can be viewed using the light microscope. For compound microscope, firstly,
after switch on the microscope we will see an ocular micrometer through the eyepiece. Then
we will put the stage micrometer slide to calibrate it with the ocular micrometer. Both
micrometer should be align in parallel with each other. The repeat with other size of
magnification but only adjust the fine focus. For stereoscopic, after switch on the microscope
the observation how it is functioning was observed. After calibrate, we change the stage slide
with other type of sample given. A prepared sample can be viewed using compound
microscope to produce an enlarged image. Also, if the higher magnification used, the sized of
field view, the distance between lenses and specimen decreases. Lastly, the result of
observation was observed and compared with other groups.
2.0 INTRODUCTION
In science, there are parts of things that are curiously but too tiny to see well - in some
cases to see at all - with the bare eye. A amplifying focal point is nice, but a arrangement of
focal points, each amplifying the picture of the final, works way better. Thus, we use
microscope as an alternative to see through it. A few types of microscope exists as time goes
by but in this experiment we will learn two types of microscope \which is compound and
stereoscopic microscope.

Compound microscope is a optical instrument that consist of two lenses which magnify and
focus on the picture of specimen. It can magnify specimen up to 1000.

Stereoscopic microscope is a microscope that provide three dimensional view of specimen.

Oil Immersion Microscopy builds the refractive record of an example when utilized
appropriately. With constrained weaknesses, slides arranged with oil submersion systems
work best under higher amplification where oils increment refraction regardless of short
central lengths.
3.0 MATERIAL

1. Compound microscope

2. microscope

3. Specimen : leaf, pond water, insects and prepared slide

4. Stereoscopic Distilled water

5. Cover slips

6. Tissue paper

7. Glass slide

8. Forceps

9. Stage micrometer

10. Dropper

11. Oil immersion

TASK 1

IDENTIFY THE PARTS OF COMPOUND AND STEREOSCOPIC MICROSCOPE

1. The operational aspects of the microscope parts that enable to show a clear view of
sample was described while exploring
2. Then, the appropriates microscope was chosen to observe the sample of non-living
and living material
3. After that, a drawing of the observation on generalities about microscope function
was drawn
4. To obtain field of view, level of magnification, preparing specimen for observation,
examining the structure of samples, a drawing cell at different level of magnification
and measuring cell a method was used and been described.

TASK 2

MEASURING THE SIZE OF A SPECIMEN USING A STAGE MICROMETER AND AN

1. The objective place was placed at the lowest, then the stage micrometer was focussed
2. The ocular micrometer was rotated until the edge of scale is parallel on the stage
micrometer.
3. Next, the two scales was overlap again as the result after looked on another point.
4. Then, the space on each scale was counted from start to the second point that was
overlap
5. It will be given x ocular is equal to xmm. The number of ocular units was divided into
stage units or mm.
6. The number of mm in each ocular unit was determined
7. Lastly, mm figure was multiplied by 1000 to determine the number of mm in each
ocular unit.
 5.0 RESULTS

COMPOUND MICROSCOPE

STEREOSCOPIC MICROSCOPE
PARTS OF MICROSCOPE AND ITS FUNCTION

Eyepiece or ocular lens : Eyepiece is the lens, present at the top and is used to see the objects under
study. Eyepiece lens contains a magnification of 10X or 15X.

Tube: Tube or the body tube, connects the eyepiece to the objective lenses.

Resolving nosepiece : It is also known as the Turret. Resolving nosepiece has holders for the
different objective lenses. It allows the rotation of the lenses while viewing.

Objective lenses : Generally, three or four objective lenses are found on a microscope, with ranges of
10X, 40X, 100X powers. Lenses are colour coded, the shortest lens is of the lowest power, and the
longest lens is high power lenses.

Diaphragm : Diaphragm helps in controlling the amount of light that is passing through the opening
of the stage. It is helpful in the adjustment of the control of light that enters.

Coarse adjustment knob : Used for focus on scanning. Usually the low power lens is used enabling
the movement of the tube.

Fine adjustment knob : Used for focus on oil. Moves the body tube for focussing the high power
lens.

Arm : It supports the tube of the microscope and connects to the base of the microscope.

Stage : The platform that is flat used for placing the slides under observation.

Stage clip : Stage clips hold the slides in proper place.

Condensor : The main function of condenser lens is focussing the light on the specimen under
observation. When very high powers of 400X are used, condenser lenses are very important. Presence
of condenser lens gives a sharper image as compared to the microscope with no condenser lens.

Base : Provides basal support for the microscope.

Power switch: The main power switch that turns the illumination on or off.