International Rules for

Pre-College Science Research:

Guidelines for Science and
Engineering Fairs
2019 – 2020
 International Rules for Pre-College Science Research:
Guidelines for Science and Engineering Fairs
2019 – 2020
Table of Contents
General Requirements for All Projects
ISEF Eligibility/Limitations..........................................................................................................................................................................3
ISEF Requirements........................................................................................................................................................................................3
Continuation/Research Progression of Projects...............................................................................................................................4
Team Projects...................................................................................................................................................................................................4

Roles and Responsibilities of Students and Adults

1. ISEF Ethics Statement.................................................................................................................................................... 4
2. The Student Researcher(s)..................................................................................................................................................................5
3. The Adult Sponsor...................................................................................................................................................................................5
4. The Qualified Scientist..........................................................................................................................................................................5
5. The Designated Supervisor.................................................................................................................................................................5
6. The Institutional Review Board (IRB)..............................................................................................................................................5
7. The Affiliated Fair Scientific Review Committees (SRC).........................................................................................................6
8. Regulated Research Institution/Industrial Settings Review Committees........................................................................6
9. ISEF Scientific Review Committee....................................................................................................................................................7

Human Participants Rules

1. Rules..............................................................................................................................................................................................................8
2. Exempt Studies.........................................................................................................................................................................................9
3. Human Participant Risk Assessment............................................................................................................................................ 10

Vertebrate Animals Rules

1. Rules for ALL Vertebrate Animal Studies ................................................................................................................................... 11
2. Additional Rules for Projects Conducted at School/Home/Field..................................................................................... 12
3. Additional Rules for Projects Conducted in a Regulated Research Institution .......................................................... 13

Potentially Hazardous Biological Agents Rules
Rules for ALL Projects Involving Potentially Hazardous Biological Agents......................................................................... 14
Additional Rules for Projects Involving Unknown Microorganisms....................................................................................... 15
Additional Rules for Projects Involving Recombinant DNA (rDNA) Technologies........................................................... 15
Additional Rules for Projects Involving Tissues and Body Fluids Including Blood and Blood Products.................. 15
Potentially Hazardous Biological Agents Risk Assessment ...................................................................................................... 17

Hazardous Chemicals, Activities or Devices Rules

Rules for ALL Projects Involving Hazardous Chemicals, Activities or Devices.................................................................. 18
Additional Rules for Specific Regulated Areas................................................................................................................................ 18
Hazardous Chemicals, Activities or Devices Risk Assessment................................................................................................. 20

Engineering Projects Guide ......................................................................................................................................................... 21

Sources of Information .................................................................................................................................................................. 22
Display & Safety Regulations ...................................................................................................................................................... 25
Categories & Sub-Categories...................................................................................................................................................... 28
Information on Required Abstract ........................................................................................................................................... 29
Forms .................................................................................................................................................................................................... 30

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 1
The International Rules and Guidelines for Science Fairs is available at societyforscience.org/ISEF2020 in multiple
formats. Familiarity with the rules is critical for students, parents, teachers, mentors, fair directors and local and
affiliated fair Scientific Review Committees (SRC) and Institutional Review Boards (IRB).

• International Rules and Guidelines — The full text of the International Rules and forms in html and as a
downloadable pdf.
• ISEF Rules Wizard — An interactive tool which asks questions about your intended project and provides a list of
forms required.
• Common SRC Problems — Frequent problems that emerge during Scientific Review Committee review for
qualification at ISEF. Read these to learn what NOT to do.

These Rules are applicable for:

The International Science and Engineering Fair 2020

Anaheim, CA, USA, May 10 –15, 2020

The purpose of these rules is to:

• protect the rights and welfare of the student researcher
• protect the rights and welfare of human participants
• protect the health and welfare of vertebrate animal subjects
• protect and promote good stewardship of the environment
• ensure adherence to federal regulations
• ensure use of safe laboratory practices
• determine eligibility for competition in ISEF

For pre-review and approval of your project, find your fair at:
https://findafair.societyforscience.org/
For rules questions, contact the ISEF Scientific Review Committee:
[email protected]

For general questions, contact:

Society for Science & the Public
Science Education Programs
1719 N Street, NW, Washington, DC 20036
office: 202-785-2255, fax: 202-785-1243
email: [email protected]

Page 2 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 ALL PROJECTS

Eligibility/Limitations 7. Projects competing at ISEF must have an exhibit that adheres

to ISEF Display & Safety requirements and is visible during all
1. Each ISEF-affiliated fair may send to ISEF the number of projects operable hours of the exhibit hall without reliance on electricity or
allocated and committed to within their affiliation agreement. internet connections.

2. A student must be selected by an ISEF-affiliated fair, and meet 8. All projects must adhere to the requirements of the affiliated
both of the following: fair(s) in which it competes to qualify for participation in ISEF.
a. be in grades 9 –12 or equivalent; and Affiliated fairs may have additional restrictions or requirements.
b. not have reached age 20 on or before May 1 preceding ISEF. Knowledge of these requirements is the responsibility of the
student and Adult Sponsor.
3. English is the official language of ISEF. Student project boards and
abstracts must be in English.
Approval and Documentation
1. Before experimentation begins, a local or regional Institutional
4. Each student is only allowed to enter one project. That project
Review Board (IRB) or Scientific Review Committee (SRC)
may include no more than 12 months of continuous research and
associated with the ISEF-affiliated fair must review and approve
may not include research performed before January 2019.
most projects involving human participants, vertebrate animals,
and potentially hazardous biological agents. Note: If a project
5. Team projects must have no more than three members. Teams
involves the testing of a student designed invention, prototype
competing at ISEF must be composed of the original members
or concept by a human, an IRB review and approval may be
who competed at the ISEF-affiliated fair and must all meet ISEF
required prior to experimentation. See Human Participants Rules
eligibility.
for details.
6. Students may compete in only one ISEF affiliated fair, except when
2. Every student must complete the Student Checklist (1A), a
proceeding to a state/national fair affiliated with ISEF from an
Research Plan/Project Summary and Approval Form (1B) and
affiliated regional fair.
review the project with the Adult Sponsor in coordination with
completion by the Adult Sponsor of the Checklist for Adult
7. Projects that are demonstrations, ‘library’ research or
Sponsor (1).
informational projects, ‘explanation’ models are not recommended
or appropriate for ISEF.
3. A Qualified Scientist is required for all studies involving Biosafety
Lab-2 (BSL-2) potentially hazardous biological agents and DEA-
8. All sciences and engineering disciplines are represented at ISEF
controlled substances and is also required for many human
and projects compete in one of the 22 categories. Review a
participant studies and many vertebrate animal studies.
complete list of categories and sub-categories with definitions.
4. After initial IRB/SRC approval (if required), any proposed changes
9. A research project may be a part of a larger study performed by
in the Student Checklist (1A) and Research Plan/Project Summary
professional scientists, but the project presented by the student
must be re-approved before laboratory experimentation/data
must be only their own portion of the complete study.
collection resumes.
Requirements
5. Projects which are continuations of a previous year’s work and
General
which require IRB/SRC approval must undergo the review process
1. All domestic and international students competing in an ISEF-
with the current year ResearchPlan/Project Summary prior to
affiliated fair must adhere to all rules as set forth in this document.
experimentation/data collection for the current year.
2. All projects must adhere to the Ethics Statement above.
6. Any continuing project must document that the additional research is
new and different. (Continuation/Research Progression Projects Form
3. It is the responsibility of the student and the Adult Sponsor to
(7)).
evaluate the study to determine if the research will require forms
and/or review and approval prior to experimentation.
7. If work was conducted in a regulated research institution,
industrial setting or any work site other than home, school or field
4. Projects must adhere to local, state and U.S. Federal laws,
at any time during the current ISEF project year, the Regulated
regulations and permitting conditions. In addition, projects
Research Institutional/Industrial Setting Form (1C) must be
conducted outside the U.S. must also adhere to the laws of the
completed and displayed at the project booth.
country and jurisdiction in which the project was performed.
8. After experimentation, each student or team must submit a
5. The use of non-animal research methods and alternatives to
(maximum) 250-word, one-page abstract which summarizes
animal research are strongly encouraged and must be explored
the current year’s work. The abstract must describe research
before conducting a vertebrate animal project.
conducted by the student, not by the supervising adult(s).
6. Introduction or disposal of non-native, genetically-altered, and/or
9. A project data book and research paper are not required, but are
invasive species (e.g. insects, plants, invertebrates, vertebrates),
strongly recommended for judging purposes. Regional or local
pathogens, toxic chemicals or foreign substances into the
fairs may require a project data book and/or a research paper.
environment is prohibited. It is recommended that students
reference their local, state or national regulations and quarantine
lists.
International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 3
10. All signed forms, certifications, and permits must be available for an affiliated fair of one of its members, but not at multiple fairs.
review by all regional, state, national and international affiliated However, each affiliated fair holds the authority to determine
fair SRCs in which the student(s) participate. This review must whether teams with members outside of a fair’s geographic
occur after experimentation and before competition. territory are eligible to compete, understanding that if the team
wins the right to attend ISEF, all team members’ expenses must be
Digital Paperwork and Signatures supported by the fair.
Submission of forms generated by a digital system are allowable a. Team membership cannot be changed during a given
under the following conditions: research year unless there are extenuating circumstances
1. The forms must have the same content and order as ISEF forms. and the local SRC reviews and approves the change,
including converting a team project to an individual project
2. Digital signatures must have a verification system via login and or vice versa. Such conversions must address rationale
have a time and date stamp to indicate this authentication. for the change and include a clear delineation between
research preceding the change and that which will follow. A
3. Paperwork submitted to Society for Science & the Public for ISEF memorandum documenting this review and approval should
must be scanned and submitted via the online portal. be attached to Form 1A.
b. Once a project has competed in a science fair at any level,
team membership cannot change and the project cannot be
Continuation/Research Progression of Projects converted from an individual project to a team project or
1. As in the professional world, research projects may build on work vice versa.
performed previously. A valid continuation project is a sound c. In a future research year, any project may be converted from
scientific endeavor. Students will be judged only on laboratory an individual to a team project, from a team to an individual
experiment/data collection performed over 12 continuous months project and/or have a change in team membership.
beginning no earlier than January 2019 and ending May 2020.
3. Each team is encouraged to appoint a team leader to coordinate
2. Any project based on the student’s prior research could be the work and act as spokesperson. However, each member of the
considered a continuation/research progression project. team should be able to serve as spokesperson, be fully involved
These projects must document that the additional research with the project, and be familiar with all aspects of the project.
is a substantive expansion from prior work (e.g. testing a new The final work should reflect the coordinated efforts of all team
variable or new line of investigation). Repetition of previous members and will be evaluated using the same judging criteria as
experimentation with the same methodology and research individual projects.
question, even with an increased sample size, is an example of an
unacceptable continuation. 4. Each team member must submit an Approval Form (1B). Team
members must jointly submit the Checklist for Adult Sponsor (1),
3. The display board and abstract must reflect the current year’s one abstract, a Student Checklist (1A), a Research Plan/Project
work only. The project title displayed in the finalist’s booth may Summary and other required forms.
mention years (for example, “Year Two of an Ongoing Study”).
Previous year’s databooks, research papers and supporting 5. Full names of all team members must appear on the abstract and
documents may be at the booth if properly labeled as such. forms.

4. Longitudinal studies are permitted as an acceptable continuation

under the following conditions: Roles and Responsibilities of Students and
a. The study is a multi-year study testing or documenting the Adults
same variables in which time is a critical variable. (Examples:
Effect of high rain or drought on soil in a given basin, return
of flora and fauna in a burned area over time.) Ethics Statement
b. Each consecutive year must demonstrate time-based Student researchers, as well as adults who have a role in their
change. projects, are expected to maintain the highest ethical standards.
c. The display board must be based on collective past These include, but are not limited to:
conclusionary data and its comparison to the current year • Integrity. Honesty, objectivity, and avoidance of conflicts of
data set. No raw data from previous years may be displayed. interest are expected during every phase of the research.
The project should reflect independent research done by the
5. All projects must be reviewed and approved each year and forms student(s), and represent only one year’s work.
must be completed for the new year. • Legality. Compliance with all federal, country, state and local
laws is essential. All projects must be approved by a Scientific
6. NOTE: For competition in ISEF, the Continuation Research Review Committee (SRC), and when necessary must also be
Progression Project Form (7) is required for projects in the same approved by an Institutional Review Board (IRB), Institutional
field of study as a previous project. This form must be displayed Animal Care and Use Committee (IACUC), and/or Institutional
at the project booth. Retention of all prior years’ paperwork is Biosafety Committee (IBC).
required and must be presented to the ISEF SRC upon request. • Respect for Confidentiality and Intellectual Property.
Confidential communications, as well as patents, copyrights,
Team Projects and other forms of intellectual property must be honored.
1. Team projects compete and are judged in the category of their Unpublished data, methods, or results may not be used without
research at ISEF. All team members must meet the eligibility permission, and credit must be given to all contributions to
requirements for ISEF. research.
• Stewardship of the Environment. It is the responsibility of the
2. Teams must have no more than three members. A team with researcher(s) and the adults involved to protect the environment
members from different geographic regions may compete at and its organisms from harm. All projects involve some amount

Page 4 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 of risk. Everyone is expected to recognize the hazards, assess the The Qualified Scientist (QS)
risks, minimize them, and prepare for emergencies. Qualifications:
• Animal Care. Proper care and respect must be given to • Earned a doctoral/professional degree in a scientific discipline
vertebrate animals. The guiding principles for the use of animals related to student’s area of research
in research includes the following “Four R’s”: Replace, Reduce, AND/OR
Refine, Respect. • Individual with extensive experience and expertise in the
• Human Participant Protection. The highest priority is the student’s area of research
health and well-being of the student researcher(s) and human • Must be thoroughly familiar with the following regulations that
participants. govern the student’s area of research including all local, state,
• Potentially Hazardous Biological Agents (PHBAs). It is the Federal and if applicable, non-U.S. national regulations and laws.
responsibility of the student and adults involved in the project • Can also serve as the Adult Sponsor, if that person meets both
to conduct and document a risk assessment, and to safely handle sets of qualifications
and dispose of organisms and materials. • May live elsewhere and not be local to the student, in which case,
a Designated Supervisor has been appointed and trained to serve
Scientific fraud and misconduct are not condoned at any level of as the onsite supervision as necessary for the specific student
research or competition. This includes plagiarism, forgery, use or project.
presentation of other researcher’s work as one’s own and fabrication
of data. Fraudulent projects will fail to qualify for competition in Responsibilities:
affiliated fairs and ISEF. Society for Science and the Public reserves The Qualified Scientist is responsible for:
the right to revoke recognition of a project subsequently found to • Reviewing the ISEF rules relevant to the project and approving
have been fraudulent. the student’s research plan or engineering design prior to the
start of experimentation
The Student Researcher(s) • Providing direct supervision throughout the timeline of the
The student researcher is responsible for all aspects of the research project or coordinating with a Designated Supervisor to serve in
project : this capacity
• Enlisting the aid of any required supervisory adults (Adult • Ensuring the proper training of the Student Researcher and/or
Sponsor, Qualified Scientist, etc.), obtaining necessary approvals Designated Supervisor in the necessary procedures
(SRC, IRB, etc.) • Completing the required documentation which may include the
• Following the International Rules & Guidelines and obtaining Regulated Research Institutional Setting Form (1C), the Qualified
all necessary approvals (SRC, IRB, etc.) and completing all Scientist Form (2) and the Risk Assessment Form (3), when
appropriate documentation applicable.
• Performing the project (which may include, but is not limited to)
experimentation, data collection, engineering, data analysis, and The Designated Supervisor (DS)
any other process or procedures related to the project Qualifications:
• Understanding and abiding by the Ethics Statement and attesting • Does not need an advanced degree
to this understanding on Approval Form 1B. • Must be familiar with the student’s project and agree to any
training necessary
To avoid conflict of interest, no Adult Sponsor, parent or other relative • May also serve as the Adult Sponsor for the project
of the student, the Qualified Scientist, or Designated Supervisor who • If the project involves the use of Vertebrate Animals
oversees the project, may serve on the SRC or IRB reviewing that project. (where behavior/habitat is influenced by humans), must be
knowledgeable about the humane care and handling of the
The Adult Sponsor animals
Qualifications:
• An Adult Sponsor may be a teacher, parent, professor, and/or Responsibilities:
other professional scientist • Providing direct supervision of the student experimentation
• Should be knowledgeable in the area of student research, be • Completing the required documentation — the Designated
familiar with the regulations around procedures and materials Supervisor box on the Qualified Scientist Form (2) when
that apply to the student project, particularly when involving applicable
human participants, vertebrate animals, potentially hazardous • Reviewing and completing the Risk Assessment Form (3) when
biological agents or hazardous chemicals, devices or activities. needed
• Should have close contact with the student throughout the
timeline of the project.
Review Committees
Responsibilities: The Institutional Review Board (IRB)
The Adult Sponsor is responsible for: An Institutional Review Board (IRB), is a committee that, according
• Working with the student to evaluate any possible risks involved to federal regulations (45-CFR-46), must evaluate the potential
in order to ensure the health and safety of the student conducting physical and/or psychological risk of research involving humans. All
the research and the humans and/or animals involved in the study. proposed human research must be reviewed and approved by an IRB
• Reviewing the student’s Student Checklist (1A) and Research before experimentation begins. This includes review of any surveys or
Plan/Project Summary to ensure that: questionnaires to be used in a project.
• experimentation follows local, state, and Federal laws and
ISEF rules Federal regulations require local community involvement. Therefore,
• forms are completed by other required adults it is advisable that an IRB be established at the school level to
• any required Qualified Scientist meets the criteria as set evaluate human research projects. If necessary, the local or ISEF-
forth in the ISEF Rules and Guidelines affiliated SRC can serve as an IRB as long as it has the required
• the student’s research is eligible for entry in ISEF membership. An IRB must consist of a minimum of three members
including the following:

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 5
• An educator An SRC must consist of a minimum of three persons, including the
• A school administrator (preferably principal or vice principal) following:
• A medical or mental health professional. The medical or mental • a biomedical scientist with an earned graduate degree
health professional may be a medical doctor, nurse practitioner, • an educator
physician’s assistant, doctor of pharmacy, registered nurse, • at least one additional member
psychologist, licensed social worker or licensed clinical professional
counselor. The medical or mental health professional on the IRB may Additional expertise: Many project evaluations require additional
change depending on the nature of the study. This person must be expertise (e.g., on biosafety and/or of human risk groups). If the SRC
knowledgeable about and capable of evaluating the physical and/or needs an expert as one of its members and one is not in the immediate
psychological risk involved in a given study. area, all documented contact with an external expert must be submitted.
If animal research is involved, at least one member must be familiar with
Additional Expertise: If an expert is not available in the immediate area, proper animal care procedures. Depending on the nature of the study, this
documented contact with an external expert is recommended. A copy of person can be a veterinarian or animal care provider with training and/or
all correspondence with the expert (e.g. emails) must be attached to Form experience in the species being studied.
4 and can be used in lieu of the signature of that expert.
To avoid conflict of interest, no Adult Sponsor, parent or other relative
To avoid conflict of interest, no Adult Sponsor, parent or other relative of the student(s), the Qualified Scientist, or the Designated Supervisor
of the student, the Qualified Scientist, or Designated Supervisor who who oversees the project may serve on the SRC reviewing that project.
oversees the project, may serve on the IRB reviewing that project. Additional members are recommended to diversify and to increase the
Additional members are recommended to help avoid a potential expertise of the committee.
conflict of interest and to increase the expertise of the committee.
A Scientific Review Committee (SRC) examines projects for the following:
IRBs exist at federally Regulated Research Institutions (e.g., universities, • Evidence of proper supervision
medical centers, NIH, correctional facilities). Prisoner advocates must • Completed forms, signatures, research dates, and preapproval dates
be included on the IRB when research participants are incarcerated. The (when required)
institutional IRB must initially review and approve all proposed research • Evidence of proper team composition
conducted at or sponsored by that institution. The Adult Sponsor and the • Compliance with rules and laws governing human and/or animal
local IRB are responsible for ensuring that the project is appropriate for a research and research involving potentially hazardous biological
pre-college student and adheres to ISEF rules. agents and/or hazardous chemicals, activities or devices
• Compliance with ISEF ethics statement
An IRB is responsible for assessing risk and documenting the • Use of accepted and appropriate research techniques
determination of risk level on Human Participant Form 4. However, in • Evidence that risks have been properly assessed
reviewing projects just prior to a fair, if the SRC serving at that level of • Evidence of search for alternatives to animal use
competition judges an IRB’s decision as inappropriate, thereby placing • Humane treatment of animals
human participants in jeopardy, they may override the IRB’s decision and • Documentation of substantial expansion for continuation projects
the project may fail to qualify for competition. It is advised that IRBs • Evidence of appropriate literature search and attribution
consult with the local or affiliated fair SRCs and/or with ISEF SRC in
questionable cases. Combined SRC/IRB Committee
A combined committee is allowed as long as the membership meets both
The Affiliated Fair Scientific Review Committee (SRC) the SRC and IRB requirements listed previously.
A Scientific Review Committee (SRC) is a group of qualified individuals
that is responsible for evaluation of student research, certifications, Regulated Research Institutions/Industrial Settings (RRI)
research plans and exhibits for compliance with the rules, applicable Review Committees
laws and regulations at each level of science fair competition. Affiliated Regulated Research Institution: A Regulated Research Institution within
Fairs may authorize local SRCs to serve in this prior review capacity. The the U.S. is defined as a professional research/teaching institution that is
operation and composition of the local and Affiliated Fair SRCs must fully regularly inspected by the USDA and is licensed to use animals covered
comply with the International Rules. Directions for obtaining preapproval by the Animal Welfare Act and may also be subject to U.S. Public Health
are available from the affiliated fair. A list of fairs is at: https://findafair. Service Policy. Also included are all federal laboratories such as National
societyforscience.org/ Institutes of Health, Veteran’s Affairs Medical Centers and the Centers
for Disease Control. In addition, pharmaceutical and biotechnology
Most proposed research projects involving vertebrate animals and/or companies and research institutions that utilize research animals that
potentially hazardous biological agents must be reviewed and approved are not covered by the Animal Welfare Act but have an operational
BEFORE experimentation. Local or regional SRC prior review is not Institutional Animal Care and Use Committee and are in compliance with
required for human studies previously reviewed and approved by a U.S. federal laws are included in this definition. For projects conducted
properly constituted IRB. outside of the United States, a Regulated Research Institution would be a
comparable research institution that adheres to country laws governing
ALL projects, including those previously reviewed and approved by an IRB the care and use of vertebrate animals.
must be reviewed and approved by the SRC after experimentation and
before competition in an Affiliated Fair. Projects which were conducted at
a Regulated Research Institution, industrial setting or any work site other
than home, school or field and which were reviewed and approved by the
proper institutional board before experimentation, must also be approved
by the Affiliated Fair SRC.

Page 6 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
Certain areas of research conducted in a regulated research institution or
an industrial setting require review and approval by federally mandated
committees that have been established at that institution. These
committees include:
• Institutional Animal Care and Use Committee (IACUC); Animal Care
and Use Committee (ACUC); Animal Ethics Committee
• Institutional Review Board (IRB); Human Subjects Participant
Program (HSPP)
• Institutional Biosafety Committee (IBC)
• Embryonic Stem Cell Research Oversight Committee (ESCRO)
• Safety Review Committee

The ISEF Scientific Review Committee (ISEF SRC)

All projects are reviewed by ISEF Scientific Review Committee prior to
competition. ISEF SRC is the final arbiter of the qualification of students to
participate in ISEF. Before the fair, committee members review research
plans and all required forms to confirm that applicable ISEF rules have
been followed. ISEF SRC may request additional information from
students prior to ISEF or may interview potential ISEF participants at the
fair to ensure that they qualify to compete.

ISEF SRC, like an Affiliated Fair SRC, is made up of adults knowledgeable

about research regulations. In addition to the review of all projects at ISEF,
committee members answer questions about the rules throughout the
year from students and teachers. The ISEF SRC can be contacted at
[email protected].

Members of ISEF Scientific Review Committee 2019:

Ms. Susan Appel

Mr. Henry Disston
Dr. Jennifer Green
Dr. Paula Johnson
Dr. Timothy Martin
Mrs. Evelyn Montalvo
Mr. Joseph Scott
Dr. Jason Shuffitt
Mrs. Andrea Spencer

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 7
 Human Participants Rules
Rules involving human participants
The following rules were developed to help pre-college student 3. The student must comply with all determinations made by the
researchers adhere to the federal regulations governing professional School or RRI IRB before beginning any interaction with human
scientists and to protect the welfare of both human participants participants (e.g., recruitment, data collection).
and the student researcher. Health and well-being is of the highest a. If the IRB requires a Qualified Scientist (QS), Form 2 must
priority when students conduct research with human participants. be completed by the QS before any interaction with human
participants. The School IRB will review this completed form
According to Code of Federal Regulation 45, CFR 46, a human before approving the project.
participant is a living individual about whom an investigator b. If the IRB requires a Designated Supervisor (DS), Form 3 must
conducting research obtains (1) data or samples through intervention be completed before any interaction with human participants.
or interaction with individuals(s) or (2) identifiable private The School IRB will review this completed form before
information. approving the project.
c. See rule #4 below regarding required procedures for
Examples of projects that are considered “human participant obtaining informed consent/assent and/or parental
research” include: permission.
• Participants in physical activities (e.g., physical exertion, ingestion
of any substance, any medical procedure) 4. Participation in research may begin only after research
• Psychological, educational and opinion studies (e.g., surveys, participants have voluntarily given informed consent/assent (in
questionnaires, tests) some cases with parental permission). Adult research participants
• Studies in which the researcher is the subject of the research may give their own consent. Research participants under 18
• Testing of student designed invention, prototype or computer years of age and/or individuals not able to give consent (e.g.
application by human participants other than student researcher developmentally disabled individuals) give their assent, with the
• Data/record review projects that include data that are not de- parent/guardian providing permission.
identified/anonymous (e.g., data set that includes name, birth date,
phone number or other identifying variables) The School IRB will determine whether the consent/assent/
• Behavioral observations that parental permission may be a) verbal or implicit or b) must be
a. involve any interaction with the observed individual(s) or written. See the Risk Assessment information on page 9 and the
where the researcher has modified the environment (e.g., online Risk Assessment Guide (https://sspcdn.blob.core.windows.
post a sign, place an object). net/files/Documents/SEP/ISEF/Resources/Risk-Assessment-
b. occur in non-public or restricted access settings (e.g., day Guide.pdf) for further explanation of informed consent.
care setting, doctor’s office) a. Informed consent requires that the researcher provides
c. involve the recording of personally identifiable information. complete information to the participant (and where
applicable, parents or guardians) about the risks and benefits
Rules associated with participation in the research study, which
1. Student researchers must complete ALL elements of the then allows the participants and parents or guardians to make
Human Participants portion of the Research Plan/Project an informed decision about whether or not to participate.
Summary Instructions and evaluate and minimize the physical, b. Participants must be informed that their participation is
psychological and privacy risks to their human participants. See voluntary and that they are free to stop participating at any
Risk Assessment information on page 11 and the online Risk time (i.e., they may participate or decline to participate, with
Assessment Guide (https://student.societyforscience.org/human- no adverse consequences of non-participation or aborted
participants#riskassess) for additional guidance. participation).
c. Informed consent may not involve coercion.
2. Student research involving human participants must be reviewed d. When written parental permission is required and the study
and approved by an Institutional Review Board (IRB) (See page includes a survey, the survey must be attached to the consent
5) before any interaction (e.g., recruitment, data collection) with form.
human participants may begin. It is the responsibility of the IRB e. The student researcher may request that the IRB waive
to evaluate potential physical and/or psychological risks of the the requirement for written informed consent/parental
project and make a determination about whether the project permission in his/her research plan if the project meets
is appropriate for student research and safe for the student specific requirements. See section on IRB waivers for more
researcher and participants. information about situations in which written parental
a. Projects that are conducted at school, at home or in the permission and/or written informed consent can be waived by
community that are not affiliated with a Regulated Research the IRB.
Institution (RRI) must be reviewed and approved by the
School IRB before the student may begin recruiting and/or 5. The research study must be in compliance with all privacy laws
interacting with human participants. The School IRB must (e.g., U.S. Family Educational Rights and Privacy Act (FERPA)
assess the risk and document its determination of risk on and the U.S. Health Insurance Portability and Accountability Act
Form 4. (HIPAA)) when they apply to the project (e.g. the project involves
b. Projects that are conducted at a Regulated Research medical information).
Institution (RRI) (e.g., university, hospital, medical center,
government lab) must have IRB approval from the RRI. A
copy of the IRB approval for the project must be obtained. A
letter from an adult mentor and/or Qualified Scientist is not
sufficient documentation of the RRI IRB review and approval
process.

Page 8 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
6. Students are prohibited from independently diagnosing disease, 3. Surveys, questionnaires, or activities that are determined by the IRB
administering medication, and/or performing medical procedures to involve perception, cognition, or game theory, etc. and that do
on human participants. NOT involve gathering personal information, invasion of privacy or
a. A student may observe and collect data for analysis of potential for emotional distress.
medical procedures, medication/treatment efficacy, and
diagnosis of illness, only under the direct supervision of a 4. Studies involving physical activity where the IRB determines that
licensed health care provider/professional. no more than minimal risk exists and where the probability and
b. This Healthcare provider/professional must be named in the magnitude of harm or discomfort anticipated in the research are not
research plan/ protocol approved by the IRB. The IRB must greater than those ordinarily encountered in DAILY LIFE or during
also confirm that the student is not violating the appropriate performance of routine physical activities.
practice act (medical, nursing, pharmacy, etc) of the state or
country in which he/she is conducting the research. If there is any uncertainty regarding the appropriateness of waiving
written informed consent/assent/parental permission, it is strongly
7. Student researchers may NOT publish or display information in a recommended that documentation of written informed consent/assent/
report that identifies the human participants directly or through parental permission be obtained.
identifiers linked to the participants (including photographs)
without the written consent of the participant(s) (Public Health
Human Participant Involvement in Student-designed
Service Act, 42, USC 241 (d)).
Invention, Prototype, Computer Application &
8. All published instruments that are not in the public domain must Engineering/Design Projects
be administered, scored and interpreted by a Qualified Scientist Student-designed invention, prototype, computer application and
as required by the instrument publisher. Any and all use and engineering/design projects that involve testing of the invention by
distribution of the test must be in accordance with the publisher’s any human participant require attention to the potential risks to the
requirements, including procurement of legal copies of the individual(s) testing or trying out the invention/prototype.
instrument. 1. IRB review and pre-approval is necessary when the student-designed
invention, prototype, application, etc. is tested by human participants
9. Studies that involve the collection of data via use of the other than the student researcher(s) or a single adult guardian or
internet (e.g., email, web-based surveys) are allowed, but Adult Sponsor/QS/DS when the testing requires an adult tester. This
researchers should be aware that they can pose challenges in a) includes surveys conducted regarding potential use, review of the
collecting anonymous data, b) obtaining informed consent and product and/or opinions regarding the project.
c) ensuring that participants are of the appropriate age to give
informed consent. See the Online Survey Consent Procedures ( 2. Projects in which the invention, prototype or project involves
https://sspcdn.blob.core.windows.net/files/Documents/SEP/ISEF/ a medical diagnosis or intervention (as defined by the FDA or
Resources/Online-Survey-Consent-Procedures.pdf). Medical Practices Act) and is tested on human participants must be
conducted at a Regulated Research Institution (RRI) with a Qualified
10. After initial IRB approval, a student with any proposed changes Scientist and receive IRB Approval from the Institution.
in the Research Plan must repeat the approval process and
regain approval before resuming interaction (recruitment, data 3. A Risk Assessment Form 3 is recommended for all student-designed
collection) with human participants. inventions or prototypes.

11. After experimentation and before competition, the Affiliated Fair Exempt Studies (Do Not Require IRB Preapproval or
SRC will review for compliance with all rules.
Human Participants Paperwork)
Some studies involving humans are exempt from IRB pre-approval
12. The following forms are required for studies involving human
or additional human participant forms. Exempt projects for ISEF and
participants:
affiliated fairs are:
a. Checklist for Adult Sponsor (1), Student Checklist (1A),
1. Student-designed Invention, Prototype, Computer Applications
Research Plan/Project Summary, and Approval Form (1B)
or Engineering/Design Project in which the student researcher
b. Human Participants Form (4) for projects reviewed by
is the only person testing the invention, prototype or computer
school IRB or IRB approval documentation from an RRI and
application and the testing does not pose a health or safety hazard.
all applicable consents and survey(s)
The only exception is a single adult guardian or Adult Sponsor/QS/
c. Regulated Research Institution Form (1C), when applicable
DS when the testing requires an adult tester. It is recommended
d. Qualified Scientist Form (2), when applicable
that a Risk Assessment Form (3) be completed.
e. Risk Assessment (3) when applicable
2. Data/record review studies (e.g., baseball statistics, crime
IRB Waiver of Written Informed Consent/Parental statistics) in which the data are taken from preexisting data sets
Permission that are publicly available and/or published and do not involve any
The IRB may waive the requirement for documentation of written interaction with humans or the collection of any data from a human
informed consent/assent/parental permission if the research involves participant for the purpose of the student’s research project.
only minimal risk and anonymous data collection and if it is one of the
following: 3. Behavioral observations of unrestricted, public settings (e.g.,
1. Research involving normal educational practices shopping mall, public park) in which all of the following apply:
a. the researcher has no interaction with the individuals being
2. Research on individual or group behavior or characteristics observed
of individuals where the researcher does not manipulate the b. the researcher does not manipulate the environment in any way
participants’ behavior and the study does not involve more than and
minimal risk. c. the researcher does not record any personally identifiable data.

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 9
4. Projects in which the student receives pre-existing/retrospective
data in a de-identified/anonymous format which complies with
both of the following conditions:
a. the professional providing the data certifies in writing that
the data have been appropriately de-identified before being
given to the student researcher and are in compliance with
all privacy and HIPAA laws, and
b. the affiliated fair SRC ensures that the data were
appropriately de-identified by review of the written
documentation provided by the supervising adult(s).

Human Participant Risk Assessment

Use this information to help determine the level of risk involved in a study involving
human participants.

All human participant projects are considered to have some level of risk.

No more than minimal risk exists when the probability and magnitude of harm or discomfort anticipated in the research are not greater
(in and of themselves) than those ordinarily encountered in everyday life or during performance of routine physical or psychological
examinations or tests.

More than minimal risk exists when the possibility of physical or psychological harm or harm related to breach of confidentiality or invasion
of privacy is greater than what is typically encountered in everyday life. Most of these studies require documented informed consent or
minor assent with the permission of parent or guardian (as applicable).

1. Examples of Greater than Minimal Physical Risk

a. Exercise other than ordinarily encountered in everyday life
b. Ingestion, tasting, smelling, or application of a substance. However, ingestion or tasting projects that involve commonly available
food or drink will be evaluated by the IRB which determines risk level based upon the nature of the study and local norms.
c. Exposure to any potentially hazardous material.

2. Examples of Greater than Minimal Psychological Risk

A research activity (e.g. survey, questionnaire, viewing of stimuli) or experimental condition that could potentially result in emotional
stress. Some examples include: answering questions related to personal experiences such as sexual or physical abuse, divorce,
depression, anxiety; answering questions that could result in feelings of depression, anxiety, or low self esteem; or viewing violent or
distressing video images.

3. Privacy Concerns
a. The student researcher and IRB must consider whether an activity could potentially result in negative consequences for the
participant due to invasion of privacy or breach of confidentiality. Protecting confidentiality requires measures to ensure that
identifiable research data are not disclosed to the public or unauthorized individuals.
b. Risk level can be reduced by protecting confidentiality or collecting data that is strictly anonymous. This requires the collection of
research in such a way that it is impossible to connect research data with the individual who provided the data.

4. Risk Groups
If the research study includes participants from any of the following groups, the IRB and student research must consider whether the
nature of the study requires special protections or accommodations:
a. Any member of a group that is naturally at-risk (e.g. pregnant women, developmentally disabled persons, economically or
educationally disadvantaged persons, individuals with diseases such as cancer, asthma, diabetes, AIDS, dyslexia, cardiac disorders,
psychiatric disorders, learning disorders, etc.)
b. Special groups that are protected by federal regulations or guidelines (e.g. children/minors, prisoners, pregnant women, students
receiving services under the Individuals with Disabilities Education Act (IDEA).
See the online Risk Assessment Guide (https://sspcdn.blob.core.windows.net/files/Documents/SEP/ISEF/Resources/Risk-
Assessment-Guide.pdf) and Online Survey Consent Procedures (https://sspcdn.blob.core.windows.net/files/Documents/SEP/ISEF/
Resources/Online-Survey-Consent-Procedures.pdf) for more detailed information on risk assessment.

Page 10 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Vertebrate Animals Rules
Rules involving vertebrate animals
The following rules were developed to help pre-college student animals during the course of experimentation; identification
researchers adhere to the federal regulations governing professional of the species, strain, sex, age, weight, source and number of
scientists and to protect the welfare of both animal subjects and the animals proposed for use.
student researcher. Health and well-being is of high priority when
students conduct research with animal subjects. 2. All vertebrate animal studies must be reviewed and approved
before experimentation begins. An Institutional Animal Care and
The Society for Science & the Public strongly endorses the use of non- Use Committee, known as an IACUC, is the institutional animal
animal research methods and encourages students to use alternatives oversight review and approval body for all animal studies at a
to animal research, which must be explored and discussed in the Regulated Research Institution. The local OR affiliated fair SRC
research plan. The guiding principles for the use of animals in research serves in this capacity for vertebrate animals studies performed
include the following “Four R’s”: in a school, home or field. Any SRC serving in this capacity must
• Replace vertebrate animals with invertebrates, lower life forms, include a veterinarian or an animal care provider with training and/
tissue/cell cultures and/or computer simulations where possible. or experience in the species being studied.
• Reduce the number of animals without compromising statistical
validity. 3. Students performing vertebrate animal research must satisfy US
• Refine the experimental protocol to minimize pain or distress to federal law as well as local, state, and country laws and regulations
the animals. of the jurisdiction in which research is performed.
• Respect animals and their contribution to research.
4. Research projects which cause more than momentary or slight
If the use of vertebrate animals is necessary, students must consider pain or distress are prohibited. Any illness or unexpected weight
additional alternatives to reduce and refine the use of animals. loss must be investigated and a veterinarian consulted to receive
required medical care. This investigation must be documented by
All projects involving vertebrate animals must adhere to the rules for the Qualified Scientist or Designated Supervisor, who is qualified
all vertebrate animal studies AND to either Section A or Section B to determine the illness, or by a veterinarian. If the illness or
rules, depending on the nature of the study and the research site. distress is caused by the study, the experiment must be terminated
immediately.
A project is considered a tissue study and not a vertebrate animal
study if tissue is obtained from an animal that was euthanized for a 5. No vertebrate animal deaths due to the experimental procedures
purpose other than the student’s project. (Use of tissues obtained are permitted in any group or subgroup.
from research conducted at a Regulated Research Institution requires a. Studies that are designed or anticipated to cause vertebrate
a copy of an IACUC certification with the name of the research animal death are prohibited.
institution, the title of the study, the IACUC approval number and b. Any death that occurs must be investigated by a
date of IACUC approval.) In tissue veterinarian, the Qualified Scientist or the Designated
studies, a student may observe the vertebrate study, but may Supervisor who is qualified to determine if the cause
not manipulate or have any direct involvement in the vertebrate of death was incidental or due to the experimental
animal experimental procedures. procedures. The project must be suspended until the cause
is determined and then the results must be documented in
Vertebrate animals, as covered by these rules, are defined as: writing.
1. Live, nonhuman vertebrate mammalian embryos or fetuses c. If death was the result of the experimental procedure, the
2. Tadpoles study must be terminated, and the study will not qualify for
3. Bird and reptile eggs starting three days (72 hours) prior to competition.
hatching
4. All other nonhuman vertebrates (including fish) at hatching or 6. All animals must be monitored for signs of distress. Because
birth. significant weight loss is one sign of stress, weight must be
Exception: Because of their delayed cognitive neural recorded at least weekly with 15% being the maximum permissible
development, zebrafish embryos may be used up to seven days weight loss or growth retardation (compared to controls) of any
(168 hours) post-fertilization. experimental or control animal. Additionally, body conditioning
scoring (BCS) systems are available for most species of animals
Rules for ALL Vertebrate Animal Studies utilized in research and are an objective method for assessing
1. All vertebrate animal studies must have a research plan that the overall health status of the research subject, with or without
includes: weight loss. A BCS system should be included in the design of
a. Justification why animals must be used, including the any study utilizing live vertebrate animals and results regularly
reasons for the choice of species, the source of animals recorded.
and the number of animals to be used; description,
explanation, or identification of alternatives to animal use 7. Students are prohibited from designing or participating in an
that were considered, and the reasons these alternatives experiment associated with the following types of studies on
were unacceptable; explanation of the potential impact or vertebrate animals:
contribution this research may have on the broad fields of a. Induced toxicity studies with known toxic substances that
biology or medicine. could cause pain, distress, or death, including but not limited
b. Description of how the animals will be used. Include methods to alcohol, acid rain, pesticides, or heavy metals or studies
and procedures, such as experimental design and data with the intent to study toxic effects of a substance on a
analysis; description of the procedures that will minimize vertebrate animal.
the potential for discomfort, distress, pain and injury to the

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 11
 b. Behavioral experiments using conditioning with aversive • Guide for the Care and Use of Agricultural Animals in
stimuli, mother/infant separation or induced helplessness. Agricultural Research and Teaching (Ag-Guide)
c. Studies of pain. • Quality Assurance Manuals (for the appropriate species)
d. Predator/vertebrate prey experiments.
3. The local or affiliated fair Scientific Review Committee must
8. Justification is required for an experimental design that involves determine if a veterinarian’s certification of the research and
food or fluid restriction and must be appropriate to the species. If animal husbandry plan is required. This certification, as well
the restriction exceeds 18 hours, the project must be reviewed and as SRC approval, is required before experimentation and is
approved by an IACUC and conducted at a Regulated Research documented on Vertebrate Animal Form 5A. A veterinarian
Institution (RRI). must certify experiments that involve supplemental nutrition,
administration of prescription drugs and/or activities that would
9. Animals may not be captured from or released into the wild not be ordinarily encountered in the animal’s daily life.
without approval of authorized wildlife or other regulatory
officials. All appropriate methods and precautions must be used 4. If an illness or emergency occurs, the affected animal(s) must
to decrease stress. Fish may be obtained from the wild only if the receive proper medical or nursing care that is directed by a
researcher releases the fish unharmed, has the proper license, and veterinarian. A student researcher must stop experimentation
adheres to state, local and national fishing laws and regulations. if there is unexpected weight loss or death in the experimental
The use of electrofishing is permissible only if conducted by a subjects. The experiment can only be resumed if the cause of
trained supervisor; students are prohibited from performing illness or death is not related to the experimental procedures and
electrofishing. if appropriate steps are taken to eliminate the causal factors. If
death is the result of the experimental procedure, the study must
10. A Qualified Scientist or Designated Supervisor must directly be terminated, and the study will not qualify for competition.
supervise all research involving vertebrate animals, except for
observational studies. 5. The final disposition of the animals must be conducted in a
responsible and ethical manner, and must be described on
11. After initial SRC approval, a student with any proposed changes Vertebrate Animal Form 5A.
in the Research Plan/Project Summary of the project must repeat
the approval process before laboratory experimentation/data 6. Euthanasia for tissue removal and/or pathological analysis is not
collection resumes. permitted for a project conducted in a school/home/field site.

A. Additional Rules for Projects Conducted at School/ 7. Livestock or fish raised for food using standard agricultural/
Home/Field aquacultural production practices may be euthanized by a
Vertebrate animal studies may be conducted at a home, school, farm, qualified adult for carcass evaluation.
ranch, in the field, etc. This includes:
1. Studies of animals in their natural environment. 8. The following forms are required:
2. Studies of animals in zoological parks. a. Checklist for Adult Sponsor (1), Student Checklist (1A),
3. Studies of livestock that use standard agricultural practices. Research Plan/Project Summary, and Approval Form (1B)
4. Studies of fish that use standard aquaculture practices b. Vertebrate Animal Form (5A)
c. Qualified Scientist Form (2), when applicable
These projects must be reviewed and approved by an SRC in which
one member is either a veterinarian and/or an animal care provider/ B. Additional Rules for Projects Conducted in a
expert with training and/or experience in the species being studied. Regulated Research Institution
1. These projects must adhere to BOTH of the following guidelines: All studies not meeting the criteria in Section A that are otherwise
a. The research involves only agricultural, behavioral, permissible under ISEF rules must be conducted in a Regulated
observational or supplemental nutritional studies on animals. Research Institution (RRI). A Regulated Research Institution within
AND the U.S. is defined as a professional research/teaching institution
b. The research involves only non-invasive and non- intrusive that is regularly inspected by the USDA and is licensed to use
methods that do not negatively affect an animal’s health or animals covered by the Animal Welfare Act and may also be subject
well-being. to U.S. Public Health Service Policy. Also included are all federal
laboratories such as National Institutes of Health, Veteran’s Affairs
All vertebrate animal studies that do not meet the criteria in Medical Centers and the Centers for Disease Control. In addition,
Section A. must be conducted in a Regulated Research Institution pharmaceutical and biotechnology companies and research
(see Section B). institutions that utilize research animals that are not covered by the
Animal Welfare Act but have an operational Institutional Animal
2. Animals must be treated kindly and cared for properly. Animals Care and Use Committee (IACUC) and are in compliance with U.S.
must be housed in a clean, ventilated, comfortable environment federal laws are included in this definition. For projects conducted
appropriate for the species. They must be given a continuous, outside of the United States, a Regulated Research Institution would
clean (uncontaminated) water and food supply. Cages, pens be a comparable research institution that adheres to country laws
and fish tanks must be cleaned frequently. Proper care must be governing the care and use of vertebrate animals.
provided at all times, including weekends, holidays, and vacation
periods. Animals must be observed daily to assess their health Some protocols permitted in a Regulated Research Institution are not
and well-being. A Designated Supervisor is required to oversee permitted for participation in ISEF; adherence to RRI rules is necessary but
the daily husbandry of the animals. Any of the following U.S. may not be sufficient.
documents provide further guidance for animal husbandry: 1. The Institutional Animal Care and Use Committee (IACUC) or the
• Federal Animal Welfare Regulation comparable animal oversight committee must approve all student
• Guide for the Care and Use of Laboratory Animals research projects before experimentation begins. Such research

Page 12 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 projects must be conducted under the responsibility of a principal
investigator. The local and affiliated fair SRCs must also review
the project to certify that the research project complies with ISEF
Rules. This local and regional SRC review should occur before
experimentation begins, if possible.

2. Student researchers are prohibited from performing euthanasia.

Euthanasia at the end of experimentation for tissue removal and/
or pathological analysis is permitted. All methods of euthanasia
must adhere to current American Veterinary Medical Association
(AVMA) Guidelines.

3. Research projects that cause more than momentary or slight pain

or distress to vertebrate animals are prohibited unless mitigated
by IACUC-approved anesthetics, analgesics and/or tranquilizers.

4. Research in nutritional deficiency or research involving

substances or drugs of unknown effect is permitted to the point
that any clinical sign of distress is noted. In the case that distress
is observed, the project must be suspended and measures must be
taken to correct the deficiency or drug effect. A project can only
be resumed if appropriate steps are taken to correct the causal
factors.

5. The following forms are required:

a. Checklist for Adult Sponsor (1), Student Checklist (1A),
Research Plan/Project Summary, and Approval Form (1B)
b. Regulated Research Institution Form (1C)
c. Qualified Scientist Form (2)
d. Vertebrate Animal Form (5B)
e. PHBA Risk Assessment Form (6A) – for all studies involving
tissues and body fluids.
f. Human and Vertebrate Animal Tissue Form (6B) – for all
studies involving tissues and body fluids.

Sources of Information are available as a separate section at the end

of the document.

Exempt Studies (Do Not Require SRC Preapproval)

1. Studies involving behavioral observations of animals are exempt
from prior SRC review if ALL of the following apply:
a. There is no interaction with the animals being observed,
b. There is no manipulation of the animal environment in any
way, and
c. The study meets all federal and state agriculture, fish, game
and wildlife laws and regulations.

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 Potentially Hazardous Biological Agents (PHBA) Rules
Potentially Hazardous Biological Agents Rules for use of microorganisms (including bacteria, viruses, viroids, prions, rickettsia, fungi
and parasites), recombinant DNA technologies or human or animal fresh/frozen tissues, blood, or body fluids.

Students are permitted to do research projects with potentially justification for usage and be conducted at a Regulated Research
hazardous biological agents meeting the conditions and rules Institution laboratory with a minimum of BSL-2 containment and
described below which were designed to protect students and to documented IBC review and approval. Representative examples
ensure adherence to federal and international biosafety regulations include, but are not limited to the following known agents:
and guidelines. MRSA (Methicillin-Resistant Staphylococcus aureus), VISA/VRSA
(Vancomycin Intermediate or Resistant Staphylococcus aureus),
When dealing with potentially hazardous biological agents, it is VRE (Vancomycin-Resistant Enterococci), CRE (Carbapenem
the responsibility of the student and all of the adults involved in a Resistant Enterobacteriacae), ESBLs (Extended Spectrum Beta-
research project to conduct and document a risk assessment on Form Lactamase producing gram negative organisms), and fungi (yeasts
(6A) to define the potential level of harm, injury or disease to plants, or molds) with known resistance to antifungal agents.
animals and humans that may occur when working with biological
agents. The risk assessment determines a biosafety level which in 8. Insertion of antibiotic resistance markers for the clonal selection
turn determines if the project can proceed, and if so, the proposed of bioengineered organisms is permitted, with the following
laboratory facility is properly equipped and all personnel are trained exceptions:
and appropriate supervision is planned. a. Students are prohibited from the insertion of antibiotic-
resistance traits or selection of organisms expressing traits
All projects involving microorganisms, recombinant DNA that may affect the ability to provide effective treatment of
technologies and human or animal fresh/frozen tissues, blood or body infections acquired by humans, animals, or plants.
fluids must adhere to the rules below AND, depending on the study, to b. Students are prohibited from designing or selecting for
the additional rules in Section A, B or C. multiple drug resistant organisms (MDROs) to investigate
the pathology, development, or treatment of antibiotic-
Rules for ALL Studies with Potentially Hazardous resistant infections.
Biological Agents (PHBA) 9. Extreme caution must be exercised when selecting and sub-
1. Prior review and approval is required for the use of potentially
culturing antibiotic-resistant organisms. Studies using such
hazardous microorganisms (including bacteria, viruses, viroids,
organisms, including BSL-1 organisms that may have originally
prions, rickettsia, fungi, and parasites), recombinant DNA (rDNA)
been exempt from prior SRC approval, require at least BSL-2
technologies or human or animal fresh/frozen tissues, blood, or
containment.
body fluids.
10. The culturing of human or animal waste, including sewage sludge,
2. An affiliated fair SRC, an IBC or an IACUC must approve
is considered a BSL-2 study.
all research before experimentation begins. The initial risk
assessment determined by the student researcher and adults
11. Naturally-occurring plant pathogens may be studied (not
supervising the project must be confirmed by the SRC, IBC or
cultured) at home, but may not be introduced into a home/garden
IACUC.
environment.
3. Experimentation involving the culturing of potentially hazardous
12. All potentially hazardous biological agents must be properly
biological agents, even BSL-1 organisms, is prohibited in a home
disposed at the end of experimentation in accordance with their
environment. However, specimens may be collected at home as
biosafety level. For BSL 1 or BSL 2 organisms: Autoclave at 121
long as they are immediately transported to a laboratory with the
degrees Celsius for 20 minutes, use of a 10% bleach solution (1:10
BSL containment determined by the affiliated fair SRC.
dilution of domestic bleach), incineration, alkaline hydrolysis,
biosafety pick-up and other manufacturer recommendations are
4. Research determined to be at Biosafety Level 1 (BSL-1) must be
acceptable.
conducted in a BSL-1 or higher laboratory. The research must
be supervised by a trained Designated Supervisor or a Qualified
13. Any proposed changes in the Research Plan/Project Summary by
Scientist. The student must be properly trained in standard
the student after initial local or affiliated fair SRC approval must
microbiological practices.
undergo subsequent SRC or IBC review and approval before such
changes are made and before experimentation resumes.
5. Research determined to be a Biosafety Level 2 (BSL-2) must
be conducted in a laboratory rated BSL-2 or above (commonly
14. The following forms are required:
limited to a Regulated Research Institution). The research must be
a. Checklist for Adult Sponsor (1), Student Checklist (1A),
reviewed and approved by the Institutional Biosafety Committee Research Plan/Project Summary, and Approval Form (1B)
(IBC) if the Regulated Research Institution requires the review. b. Regulated Research Institution Form (1C) - when applicable
For a high school BSL-2 laboratory, the SRC must review and c. Qualified Scientist (2), when applicable
approve. The research must be supervised by a Qualified Scientist. d. Risk Assessment (3), when applicable
e. PHBA Risk Assessment Form (6A), when applicable
6. Students are prohibited from designing or participating in BSL-3 or f. Human and Vertebrate Animal Tissue Form (6B) – for all
BSL-4 Research. studies involving tissues and body fluids.

7. Laboratory studies designed to culture known clinically significant

multidrug resistant organisms (MDROs) must have a written

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A. Additional Rules for Projects Involving Unknown C. Additional Rules for Projects with Tissues and Body
Microorganisms Fluids, including Blood and Blood Products
Studies involving unknown microorganisms present a challenge Studies involving fresh/frozen tissue, blood or body fluids
because the presence, concentration and pathogenicity of obtained from humans and/or vertebrates may contain
possible agents are unknown. In science fair projects, these microorganisms and have the potential of causing disease.
studies typically involve the collection and culturing of Therefore, a proper risk assessment is required.
microorganisms from the environment (e.g. soil, household
surfaces, skin.) 1. Research involving human and/or non-human primate established
cell lines and tissue culture collections (e.g., obtained from the
1. Research with unknown microorganisms can be treated as a BSL-1 American Type Culture Collection) must be considered a BSL-1
study under the following conditions: or BSL-2 level organism as indicated by source information and
a. Organism is cultured in a plastic petri dish (or other treated accordingly. The source and/or catalog number of the
standard sterile non-breakable container) and sealed. cultures must be identified in the Research Plan/Project Summary.
b. Experiment involves only procedures in which the petri dish
remains sealed throughout the experiment (e.g., counting 2. If tissues are obtained from an animal that was euthanized for a
presence of organisms or colonies). purpose other than the student’s project, it may be considered a
c. The sealed petri dish is disposed of via autoclaving or tissue study.
disinfection under the supervision of the Designated a. Use of tissues obtained from research conducted at
Supervisor. a Regulated Research Institution requires a copy of
the IACUC certification with the name of the research
2. If a culture container with unknown microorganisms is opened for institution, the title of the study, the IACUC approval
any purpose, (except for disinfection/disposal), it must be treated number and date of IACUC approval.
as a BSL-2 study and involve BSL-2 laboratory precautions. b. Use of tissues obtained from agricultural/aquacultural
studies require prior SRC approval.
B. Additional Rules for Projects Involving Recombinant
DNA (rDNA) Technologies 3. If the animal was euthanized solely for the student’s project,
Studies involving rDNA technologies in which microorganisms, the study must be considered a vertebrate animal project and
plants and/or animals have been genetically modified require is subject to the vertebrate animal rules. (See vertebrate animal
close review to assess the risk level assignment. Some rDNA rules.)
studies can be safely conducted in a BSL-1 high school laboratory
with prior review by a SRC. 4. The collection and examination of fresh/frozen tissue
and/or body fluids, (not including blood or blood products; see
1. All rDNA technology studies involving BSL-1 organisms and BSL-1 rule 7) from a non-infectious source with little likelihood of
host vector systems, including commercially available kits, must be microorganisms present must be considered Biosafety level 1
conducted in a BSL-1 laboratory under the supervision of a Qualified studies and must be conducted in a BSL-1 laboratory or higher and
Scientist or Designated Supervisor and must be approved by the SRC must be supervised by a Qualified Scientist or trained Designated
prior to experimentation. Examples include cloning of DNA in E. coli Supervisor.
K–12, S. cerevesiae, and B. subtilis host-vector systems.
5. The collection and examination of fresh/frozen tissues or
2. An rDNA technology study using BSL-1 agents that may convert body fluids or meat and meat by-products NOT obtained
to BSL-2 agents during the course of experimentation must be from food stores, restaurants, or packing houses may contain
conducted entirely in a BSL-2 facility. microorganisms. Because of the increased risk from unknown
potentially hazardous agents, these studies must be considered
3. All rDNA technology studies involving BSL-2 organisms and/ biosafety level 2 studies conducted in a BSL-2 laboratory under
or BSL-2 host vector systems must be conducted in a Regulated the supervision of a Qualified Scientist.
Research Institution and approved by the IBC prior to
experimentation, where applicable. 6. Human breast milk of unknown origin, unless certified free of
HIV and Hepatitis C, and domestic unpasteurized animal milk are
4. Propagation of recombinants containing DNA coding for human, considered BSL-2.
plant or animal toxins (including viruses) is prohibited.
7. All studies involving human or wild animal blood or blood products
5. All genome editing studies that include alteration of germline cells, should be considered at a minimum a Biosafety level 2 study and
insertion of gene drives, use of rapid trait development systems must be conducted in a BSL-2 laboratory under the supervision of
(RTDS®), etc., should be categorized as a BSL-2 study and must be a Qualified Scientist. Known BSL-3 or BSL-4 blood is prohibited.
conducted at an RRI and approved by the IBC from the institution. Studies involving domestic animal blood may be considered a
Qualified scientists are expected to ensure that student research BSL-1 level study. All blood must be handled in accordance with
protocols address appropriate intrinsic and extrinsic containment standards and guidelines set forth in the OSHA, 29CFR, Subpart Z.
precautions. Any tissue or instruments with the potential of containing blood-
borne pathogens (e.g. blood, blood products, tissues that release
6. Introduction or disposal of non-native, genetically-altered, and/ blood when compressed, blood contaminated instruments) must
or invasive species (e.g. insects or other invertebrates, plants, be properly disposed after experimentation.
vertebrates), pathogens, toxic chemicals or foreign substances
into the environment is prohibited. Students and adult sponsors 8. Studies of human body fluids, where the sample can be identified
should reference their local, state and national regulations and with a specific person, must have IRB review and approval, and
quarantine lists. informed consent.

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 15
9. Any study involving the collection and examination of body fluids Exempt Tissues (no SRC pre-approval required)
that may contain biological agents belonging to BSL-3 or BSL-4 is 1. The following types of tissue do not need to be treated as
prohibited. potentially hazardous biological agents:
a. Plant tissue (except those known to be toxic or
10. A project involving a student researcher using their own body hazardous)
fluids (if not cultured) b. Plant and non-primate established cell lines and tissue
a. can be considered a BSL-1 study culture collections (e.g., obtained from the American
b. may be conducted in a home setting Type Culture Collection). The source and/or catalog
c. must have IRB review if the body fluid is serving as a number of the cultures must be identified in the
measure of an effect of an experimental procedure on Research Plan/Project Summary.
the student researcher (e.g. Student manipulates diet and c. Fresh or frozen meat, meat by-products obtained from
takes a blood or urine sample). An example of a project not food stores, restaurants, or packing houses and eggs or
needing IRB review would be collecting urine to serve as a pasteurized milk.
deer repellent. d. Hair, hooves, nails and feathers
d. must receive prior SRC review and approval prior to e. Teeth that have been sterilized to kill any blood-borne
experimentation. pathogen that may be present.
f. Fossilized tissue or archeological specimens.
11. Studies involving embryonic human stem cells must be conducted g. Prepared fixed tissue
in a Registered Research Institution and reviewed and approved
by the ESCRO (Embryonic Stem Cell Research Oversight) Sources of Information are available as a separate section at the
Committee. end of the document.

Exempt Studies (no SRC pre-approval required)

The following types of studies are exempt from requiring SRC
pre-approval as listed below, but may be subject to additional rules
dependent upon the design of the project. Student researchers and
adult sponsors are required to refer to sections A, B, and C of this
section to review additional rules for projects that involve unknown
organisms, recombinant DNA (rDNA) technologies, tissues, fluids,
blood, or blood products before deciding upon a final biosafety level
(BSL) designation for projects.

1. The following types of studies are exempt from prior SRC review,
but require a Risk Assessment Form 3:
a. Studies involving protists and archaea.
b. Research using manure for composting, fuel production, or
other non-culturing experiments.
c. Commercially-available color change coliform detection test
kits. These kits must remain sealed and must be properly
disposed.
d. Studies involving decomposition of vertebrate organisms
(such as in forensic projects).
e. Studies with microbial fuel cells.

2. The following types of studies involve BSL-1 organisms and are

exempt from prior SRC review and require no additional forms:
a. Studies involving baker’s yeast and brewer’s yeast, except in
rDNA studies.
b. Studies involving Lactobacillus, Bacillus thuringiensis, nitrogen-
fixing, oil-eating, and algae-eating bacteria introduced into
their natural environment. (Not exempt if cultured in a petri
dish environment.)
c. Studies involving water or soil microbes not concentrated in
media conducive to their microbial growth
d. Studies of mold growth on food items if the experiment is
terminated at the first evidence of mold.
e. Studies of slime molds and edible mushrooms.
f. Studies involving E. coli k–12 (and other strains of E.
coli used solely as a food source for C. elegans) that are
performed at school and are not subject to additional rules
for recombinant DNA studies or use of antibiotic resistant
organisms.

Page 16 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Potentially Hazardous Biological Agents Risk Assessment
Use this information to complete PHBA Risk Assessment Form (6A)

Risk assessment defines the potential level of harm, injury or disease 6. Assignment of a biosafety level for the study based on risk group
to plants, animals and humans that may occur when working with of biological agent, level of biological containment available and the
biological agents. The end result of a risk assessment is the assignment expertise of the Qualified Scientist or Designated Supervisor who
of a biosafety level which then determines the laboratory facilities, will be supervising the project
equipment, training, and supervision required.
Risk assessment involves: 7. Documentation of review and approval of study prior to
experimentation:
1. Assignment of the biological agent to a risk group
a. If a study is conducted at a non-regulated site (e.g. school),
the SRC reviews the Research Plan/Project Summary.
2. Studies involving a known microorganism must begin with an initial
b. If the study was conducted at a Regulated Research
assignment of the microorganism to a biosafety level risk group
Institution, and was approved by the appropriate
based on information available through a literature search.
institutional board (e.g. IBC, IACUC), the SRC reviews the
institutional forms provided and documents SRC approval
3. The study of unknown microorganisms and the use of fresh tissues (Form(6A)).
relies on the expertise of the supervising adult(s). c. If a PHBA study was conducted at a Regulated Research
Institution but the institution does not require review
4. Determination of the level of biological containment available for this type of study, the SRC must review the study and
to the student researcher to conduct the experimentation. (See document approval on Form 6A that the student received
“Levels of Biological Containment” for details.) appropriate training and the project complies with ISEF
rules.
5. Assessment of the experience and expertise of the adult(s)
supervising the student.

Classification of Biological Agents Levels of Biological Containment

Risk Groups There are four levels of biological containment (Biosafety Level
Biological agents, plant or animal, are classified according 1–4). Each level has guidelines for laboratory facilities, safety
to biosafety level risk groups. These classifications presume equipment and laboratory practices and techniques.
ordinary circumstances in the research laboratory, or growth
of agents in small volumes for diagnostic and experimental BSL-1 containment is normally found in water-testing
purposes. laboratories, in high schools, and in colleges teaching
introductory microbiology classes. Work is done on an
BSL-1 risk group contains biological agents that pose low risk open bench or in an appropriate biosafety hood. Standard
to personnel and the environment. These agents are highly microbiological practices are used when working in the
unlikely to cause disease in healthy laboratory workers, animals laboratory. Decontamination can be achieved by treating with
or plants. The agents require Biosafety Level 1 containment. chemical disinfectants or by steam autoclaving. Lab coats and
Examples of BSL-1 organisms are: Agrobacterium tumefaciens, gloves are required. The laboratory work is supervised by an
Micrococcus leuteus, Neurospora crassa, Bacillus subtilis. individual with general training in microbiology or a related
science.
BSL-2 risk group contains biological agents that pose moderate
risk to personnel and the environment. If exposure occurs in a BSL-2 containment is designed to maximize safety when
laboratory situation, the risk of spread is limited and it rarely working with agents of moderate risk to humans and the
would cause infection that would lead to serious disease. environment. Access to the laboratory is restricted. Biological
Effective treatment and preventive measures are available safety cabinets (Class 2, type A, BSC) must be available. An
in the event that an infection occurs. The agents require autoclave should be readily available for decontaminating waste
Biosafety Level 2 containment. Examples of BSL-2 organisms materials. Lab coats and gloves are required; eye protection and
are: Mycobacterium, Streptococcus pneumoniae, Salmonella face shields must also be worn as needed. The laboratory work
choleraesuis. must be supervised by a scientist who understands the risk
associated with working with the agents involved.
BSL-3 risk group contains biological agents that usually cause
serious disease (human, animal or plant) or that can result in BSL-3 containment is required for infectious agents that
serious economic consequences. Projects in the BSL-3 group are may cause serious or potentially lethal diseases as a result
prohibited. of exposure by inhalation. Projects in the BSL-3 group are
prohibited.
BSL-4 risk group contains biological agents that usually produce
very serious disease (human, animal or plant) that is often BSL-4 containment is required for dangerous/exotic agents that
untreatable. Projects in the BSL-4 group are prohibited. pose high risk of life-threatening disease. Projects in the BSL-4
group are prohibited.

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 17
 Hazardous Chemicals, Activities or Devices Rules
Includes DEA-controlled substances, prescription drugs, alcohol & tobacco, firearms and explosives, radiation, lasers, etc.

The following rules apply to research using hazardous chemicals, A. DEA-Controlled Substances
devices and activities. These include substances and devices that are The U.S. Drug Enforcement Administration (DEA) regulates
regulated by local, state, country, or international law, most often with chemicals that can be diverted from their intended use to make
restrictions of their use by minors such as DEA-controlled substances, illegal drugs. Other countries may have similar regulatory
prescription drugs, alcohol, tobacco, firearms and explosives. bodies; students outside of the U.S. must adhere to their own
Hazardous activities are those that involve a level of risk above and country’s drug regulatory agency requirements in addition to
beyond that encountered in the student’s everyday life. U.S. DEA regulations. DEA-controlled substances and their
schedule number are at the DEA website under Sources of
These rules are intended to protect the student researcher by Information. It is the responsibility of the student to consult
ensuring proper supervision and the consideration of all potential this list if there is a possibility that substances used in
risks so that the appropriate safety precautions are taken. Students experimentation could be regulated.
are required to meet all standards imposed by ISEF, school, local, and/ 1. All studies using DEA-controlled substances must be
or regional fair(s). supervised by a Qualified Scientist who is licensed by the
DEA (or other international regulatory body) for use of the
Rules for ALL Projects Involving Hazardous Chemicals, controlled substance.
Activities and Devices
1. All projects involving hazardous chemicals, activities or devices 2. All studies using DEA Schedule 1 substances (including
must describe in the research plan the risk assessment process, marijuana) must have the research protocol approved by
supervision, safety precautions and methods of disposal. DEA before research begins. Schedule 2, 3 and 4 substances
do not require protocol approval by DEA.
2. The use of hazardous chemicals and devices and involvement in
hazardous activities require direct supervision by a Designated B. Prescription Drugs
Supervisor, except those involving DEA-controlled substances, Prescription drugs are regulated by federal or country laws to
which require supervision by a Qualified Scientist. protect against inappropriate or unsafe use. Special precautions
must be taken in their use for a science project as follows:
3. The student researcher must conduct a risk assessment in 1. Students are prohibited from administering prescription
collaboration with a Designated Supervisor or Qualified Scientist drugs to human participants.
prior to experimentation. This risk assessment is documented on
the Risk Assessment Form 3. 2. A veterinarian must supervise student administration of any
prescription drugs to vertebrate animals.
4. Student researchers must acquire and use regulated substances
in accordance with all local, state, U.S. federal and country laws. C. Alcohol and Tobacco
For further information or classification for these laws and The U.S. Alcohol and Tobacco Tax and Trade Bureau (TTB)
regulations, contact the appropriate regulatory agencies. regulates the production of alcohol and distribution of alcohol and
tobacco products. Many such products are restricted by age for
5. For all chemicals, devices or activities requiring a Federal and/or purchase, possession and consumption.
State Permit, the student/supervisor must obtain the permit prior 1. Fermentation studies in which minute quantities of ethyl
to the onset of experimentation. A copy of the permit must be alcohol are produced are permitted.
available for review by adults supervising the project and the local, 2. The Designated Supervisor is responsible for the
affiliated, and ISEF SRCs in their review prior to competition. acquisition, usage and appropriate disposal of the alcohol or
tobacco used in the study.
3. Production of wine or beer by adults is allowable in the
6. The student researcher must minimize the impact of an
home and must meet TTB home production regulations.
experiment on the environment. Examples include using minimal
Students are allowed to design and conduct a research
quantities of chemicals that will require subsequent disposal;
project, under direct parental supervision, involving the
ensuring that all disposal is done in an environmentally safe
legal production of the wine or beer.
manner and in accordance with good laboratory practices.
4. Students are prohibited from conducting experiments
where consumable ethyl alcohol is produced by distillation.
7. The following forms are required:
However, students are allowed to distill alcohol for fuel or
a. Checklist for Adult Sponsor (1), Student Checklist (1A),
other non-consumable products. To do so, the work must be
Research Plan/Project Summary and Approval Form (1B)
conducted at school or a Regulated Research Institution and
b. Regulated Research Institution Form (1C), when applicable
follow all local and country laws. See Alcohol and Tobacco
c. Qualified Scientist Form (2), when applicable
Tax and Trade Bureau (TTB) website for details.
d. Risk Assessment Form (3)

Additional Rules for Specific Regulated Areas

There are additional rules for the following regulated areas:
A. DEA-controlled Substances
B. Prescription Drugs
C. Alcohol & Tobacco
D. Firearms and Explosives
E. Regulated Drones
F. Radiation

Page 18 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
D. Firearms and Explosives
The U.S. Bureau of Alcohol, Tobacco, Firearms and Explosives
(ATF), along with state agencies, regulates the purchase and
use of firearms and explosives. A firearm is defined as a small
arms weapon from which a projectile is fired by gunpowder.
An explosive is any chemical compound, mixture or device, the
primary purpose of which is to function by explosion. Explosives
include, but are not limited to, dynamite, black powder, pellet
powder, detonators, and igniters.

The purchase of a firearm by a minor is generally unlawful.

The use of a firearm, without proper state certification, is
illegal. Students should check the training and certification
requirements of individual states and countries.
1. Projects involving firearms and explosives are allowable
when conducted with the direct supervision of a Designated
Supervisor and when in compliance with all federal, state
and local laws.

2. A fully assembled rocket motor, reload kit or propellant

modules containing more than 62.5 grams of propellant are
subject to the permitting, storage and other requirements of
federal explosive laws and regulations.

3. Potato guns and paintball guns are not considered firearms

unless they are intended to be used as weapons. However,
they must be treated as hazardous devices.

E. Regulated Drones
Projects involving unmanned aircraft systems (UAS)/drones
must follow all state, Federal and country laws. See the Federal
Aviation Administration (FAA) for more details (www.faa.gov.uas/
registration).

F. Radiation
Projects involving radionuclides (radioisotopes) and X-rays
must involve a careful examination of the risks associated with
the study and appropriate safety precautions must be taken.
Depending upon the level of exposure, radiation released from
these sources can be a health hazard.
1. All studies may not exceed the dose limits set by the Nuclear
Regulatory Commission of 0.5 mrem/hr or 100 mrem/year of
exposure.

2. If the voltage needed in the study is <10 kvolts, a risk

assessment must be conducted. The study may be done at
home or school, and SRC preapproval is not required.

3. A study using 10-25 kvolts must have a risk assessment

conducted and must be preapproved by the SRC to assess
safety. Such a study must be conducted in a metal-lined
chamber using a camera only, not direct view through glass. A
dosimeter or radiation survey meter is required to measure
radiation exposure.

4. All studies using > 25 kvolts must be conducted at an

institution with a Licensed Radiation Program and must be
preapproved by the Institutions’ Radiation Safety Officer or
the Committee which oversees the use of ionizing radiation to
ensure compliance with state and federal regulations.

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 19
 Guidance for Risk Assessment
Please find below guidance on conducting risk assessment when using the following:
• Hazardous Chemicals
• Hazardous Devices
• Radiation

1. Hazardous Chemicals refer to Safety Data Sheets provided by the vendor (SDS) to
A proper risk assessment of chemicals must include review of ensure that proper safety precautions are taken. Some SDS
the following factors: sheets (e.g., Flinn) rank the degree of hazard associated with a
a. Toxicity – the tendency of a chemical to be hazardous to chemical. This rating may assist students and adult sponsors in
health when inhaled, swallowed, injected or in contact determining risk associated with the use of a chemical.
with the skin.
b. Reactivity  ‑  the tendency of a chemical to undergo A risk assessment (documented on Form 3) must include
chemical change. proper disposal methods for the chemicals used in an
c. Flammability  ‑  the tendency of a chemical to give off experiment. The Flinn Catalog (referenced in the Sources
vapors which readily ignite when used under normal of Information section) provides information for the proper
working conditions. disposal of chemicals. If applicable, the student researcher
d. Corrosiveness  ‑  the tendency of a chemical, upon physical must incorporate in the research plan disposal procedure
contact, to harm or destroy living tissues or physical required by federal and state guidelines.
equipment.
2. Hazardous Devices
Environmentally Responsible Chemistry The documentation of risk assessment (Form 3) is required
The mission of environmentally responsible (green) when a student researcher works with potentially hazardous/
chemistry is to avoid the use or production of hazardous dangerous equipment and/or other devices, in or outside a
substances during chemical process. The principles of green laboratory setting that require a moderate to high level of
chemistry are described on the EPA website in the Sources expertise to ensure their safe usage. Some commonly used
of Information section. Whenever possible the following devices (Bunsen burners, hot plates, saws, drills, etc.) may
principles should be incorporated into the research plan. not require a documented risk assessment, assuming that
• Waste prevention the student researcher has experience working with the
• Use of the safest possible chemicals and products device. Use of other potentially dangerous devices such as
• Design of the least possible hazardous chemical high vacuum equipment, heated oil baths, NMR equipment,
syntheses and high temperature ovens must have documentation of a
• Use renewable materials risk assessment. It is recommended that all student designed
• Use catalysts in order to minimize chemical usage inventions also have documentation of a risk assessment.
• Use of solvents and reaction conditions that are safe as
possible 3. Radiation
• Maximization of energy efficiency A risk assessment (documented on Form 3) must be conducted
• Minimization of accident potential when a student’s project involves radiation beyond that
normally encountered in everyday life. Non-ionizing radiation
includes the spectrum of ultraviolet (UV), visible light, infrared
(IR), microwave (NW), radiofrequency (RF) and extremely low
When assessing risk, the type and amount of exposure to a frequency (ELF).
chemical must be considered. For example, an individual’s
allergic and genetic disposition may have an influence on the
overall effect of the chemical. The student researcher must

Page 20 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Engineering Projects Guide
Use this information to help determine the requirements of Engineering Projects and potential areas that
will require pre-approval and/or extra safety precautions.

Engineering Project Checklist

Consider the answers to the questions below. If the response
is yes, then the project may fall under more specific rules
and those sections of the International Rules & Guidelines
should be consulted.

Hazardous Chemicals, Activities and Devices †† Does your project intend to gather personal data/have
Will your project involve any of the following: a health benefit to the user?
†† DEA-controlled Substances Vertebrate Animals
†† Firearms and Explosives †† Does your project include any interaction with
†† Prescription Drugs vertebrate animals in any phase of the project? If yes,
please refer to the full Vertebrate Animal Rules.
†† Alcohol & Tobacco
†† Regulated Drones
Potentially Hazardous Biological Agents
†† Radiation
†† Does your project include any collection, examination
or handling of microorganisms, and/or fresh or frozen
Device Testing with Human Participants
tissue, primary cell cultures, blood, blood products or
†† Are you going to test your project (device, app, body fluids?
invention, prototype, etc.)? If yes, does it require
†† Are you going to culture or isolate any substance,
persons to interact with it other than yourself or
known or unknown? If yes, please refer to the full
adult sponsor/supervisor?
Potentially Hazardous Biological Agents Rules.
†† Do you intend to gather background knowledge
through a survey or interviews to understand the
potential use and needs for your project design?
†† Are you going to ask for opinions or suggestions on
your project design at any point of the project?

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 21
Sources of Information for All Projects 6. American Psychological Association
1. United States Patent and Trade Office 750 First Street, NE Washington, DC 20002-4242
Customer Service: 1-800-786-9199 (toll-free); phone: 202-336-5500; 800-374-2721
571-272-1000 (local); 571-272-9950 (TTY) www.apa.org
www.uspto.gov
www.uspto.gov/patents/process/index.jsp Information for students:
www.apa.org/science/leadership/students/information.aspx
2. European Patent Office Information regarding publications:
www.epo.org www.apa.org/pubs/index.aspx
www.epo.org/applying/basics.html
7. Educational and Psychological Testing
3. The Mad Scientist Network at Washington University School of Testing Office for the APA Science Directorate
Medicine: phone: 202-336-6000
www.madsci.org email: [email protected]
www.apa.org/science/programs/testing/index.aspx
4. ANS Task Force
www.anstaskforce.gov 8. The Children’s Online Privacy Protection Act of 1998 (COPPA) (15
U.S.C. §§ 6501–6506)
Aquatic Nuisance Species (ANS) Task Force www.ftc.gov/privacy/coppafaqs.shtm
www.anstaskforce.gov
www.anstaskforce.gov/Documents/ISEF.pdf Vertebrate Animals
Animal Care and Use
5. APHIS 1. Laboratory Animals, Institute of Laboratory Animal Research
aphis.usda.gov/aphis/home (ILAR), Commission on Life Sciences, National Research
Animal and Plant Health Inspection Service http://dels.nas.edu/ilar
Invasive Species List
2. Guide for the Care and Use of Laboratory Animals, 8th Edition (2011)
6. Invasive Species Specialist Group http://grants.nih.gov/grants/olaw/Guide-for-the-Care-and-Use-
www.issg.org of-Laboratory-Animals.pdf
The Global Invasive Species database contains invasive www.nap.edu/catalog.php?record_id=12910
species information supplied by experts from around the
world. 3. Guidelines for the Care and Use of Mammals in Neuroscience and
Behavioral Research (2003), Institute for Laboratory Animal
7. Invasive Species Information Research (ILAR).
www.invasivespeciesinfo.gov/resources/lists.shtml https://www.nap.edu/catalog/10732/guidelines-for-the-care-and-
Provides information for species declared invasive, noxious, use-of-mammals-in-neuroscience-and-behavioral-research
prohibited, or harmful or potentially harmful.
To order these ILAR publications contact:
National Academies Press
500 Fifth Street, NW
Washington, DC 20055
phone: 888-624-8373 or 202-334-3313; fax: 202-334-2451
www.nap.edu

Human Participants 4. Federal Animal Welfare Act (AWA)

1. Code of Federal Regulation (CFR), Title 45 (Public Welfare), Part 7 U.S.C. 2131-2157
46-Protection of Human Subjects (45CFR46) Subchapter A - Animal Welfare (Parts I, II, III)
https://www.hhs.gov/ohrp/regulations-and-policy/ https://www.nal.usda.gov/awic/animal-welfare-act
regulations/45-cfr-46 Document is available from:
USDA/APHIS/AC
2. Dunn, C. M. and Chadwick, G. L., Protecting Study Volunteers in 4700 River Road, Unit 84
Research, 3rd Edition (2004). Boston, MA: Thomson Centerwatch. Riverdale, MD 20737-1234
ISBN 1-930624-44-1. email: [email protected]
Can be purchased from: phone: 301-734-7833; fax: 301-734-4978
www.amazon.com http://awic.nal.usda.gov

3. NIH tutorial, “Protecting Human Research Participants” 5. Guide for the Care and Use of Agricultural Animals in Agricultural
http://phrp.nihtraining.com/files/PHRP.pdf Research and Teaching (Agri-Guide)
Association for Assessment and Accreditation of Laboratory
4. Belmont Report, April 18, 1979 Animal Care International (AAALAC International)
www.hhs.gov/ohrp/humansubjects/guidance/belmont.html https://www.aaalac.org/
https://www.aaalac.org/about/Ag_Guide_3rd_ed.pdf
5. Standards for Educational and Psychological Testing. (1999).
Washington, DC: AERA, APA, NCME. 6. Guidelines for the Use of Fish in Research (2014), American Fisheries
www.apa.org/science/programs/testing/standards.aspx Society.
www.fisheries.org
www.fisheries.org/afs/docs/policy_16.pdf

Page 22 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
7. Euthanasia Guidelines Potentially Hazardous Biological Agents
AVMA Guidelines on Euthanasia (2013) 1. American Biological Safety Association: ABSA Risk Group
American Veterinary Medical Association Classification – list of organisms
www.avma.org/KB/Policies/Documents/euthanasia.pdf www.absa.org

Alternative Research and Animal Welfare 2. American Type Culture Collection (ATCC)
1. The National Library of Medicine provides computer searches www.atcc.org
through MEDLINE:
Reference & Customer Services 3. Bergey’s Manual of Systematic Bacteriology website – follow the
National Library of Medicine links for resources and microbial databases for a collection of
8600 Rockville Pike international websites of microorganisms and cell cultures.
Bethesda, MD 20894 https://www.bergeys.org
888-FIND-NLM or 888-346-3656; 301-594-5983;
email: [email protected] 4. Biosafety in Microbiological and Biomedical Laboratories (BMBL) -
www.nlm.nih.gov 4th Edition. Published by CDC-NIH
ncbi.nlm.nih.gov/pubmed https://www.cdc.gov/labs/BMBL.html

2. National Agriculture Library (NAL) provides reference service for 5. World Health Organization Laboratory Safety Manual
materials that document a) Alternative Procedures to Animal Use www.who.int/diagnostics_laboratory/guidance/en
and b) Animal Welfare.
Animal Welfare Information Center 6. Canada – Agency of Public Health – list of non-pathogenic
National Agriculture Library organisms
10301 Baltimore Avenue, Room 410 https://www.canada.ca/en/public-health/services/laboratory-
Beltsville, MD 20705-2351 biosafety-biosecurity/pathogen-safety-data-sheets-risk-
phone: 301-504-6212, fax: 301-504-7125 assessment.html
email: [email protected]
www.nal.usda.gov/awic 7. American Society for Microbiology
https://www.asm.org
3. Institute of Laboratory Animal Resources (ILAR) provides a variety
of information on animal sources, housing and handling standards, 8. Microbiology Society
and alternatives to animal use through annotated bibliographies Charles Darwin House
published quarterly in ILAR Journal. 12 Roger Street
ILAR  ‑  The Keck Center of the National Academies London
500 Fifth Street, NW, Keck 687 WC1N 2JU
Washington, DC 20001 UK
phone: 202-334-2590, fax: 202-334-1687 [email protected]
email: [email protected] http://microbiologyonline.org
http://dels.nas.edu/ilar
9. NIH Guidelines for Research Involving Recombinant DNA Molecules.
4. Quarterly bibliographies of Alternatives to the Use of Live Published by National Institutes of Health.
Vertebrates in Biomedical Research and Testing may be obtained https://osp.od.nih.gov/wp-content/uploads/2013/06/NIH_
from: Guidelines.pdf
Specialized Information Services
NLM/NIH 10. OSHA – Occupational Health and Safety Administration
2 Democracy Plaza, Suite 510 www.osha.gov
6707 Democracy Blvd., MSC 5467
Bethesda, MD 20892-5467 Hazardous Chemicals, Activities or Devices
phone: 301-496-1131; Fax: 301- 480-3537 General Lab/Chemical Safety
email: [email protected] 1. Safety in Academic Chemistry Laboratories, Volumes 1 and 2, 2003.
https://www.sis.nlm.nih.gov Washington, DC: American Chemical Society.
http://toxnet.nlm.nih.gov/altbib.html Order from (first copy free of charge):
American Chemical Society
5. Johns Hopkins Center for Alternatives to Animal Testing (CAAT) Publications Support Services
has worked with scientists since 1981 to find new methods to 1155 16th Street, NW
replace the use of laboratory animals in experiments, reduce the Washington, DC 20036
number of animals tested, and refine necessary tests to eliminate phone: 202- 872-4000 or 800-227-5558
pain and distress. email: [email protected]
email: [email protected] www.acs.org/education
http://caat.jhsph.edu/
2. General
6. Quality Assurance Manuals (for appropriate species) Howard Hughes Medical Institute has resources for working with
Such as: cell cultures, radioactive materials and other laboratory materials.
Poultry: https://www.bordbia.ie/industry/farmers/quality/ http://www.hhmi.org/developing-scientists/resources
PoultrySchemeStandards/Poultry%20Producer.pdf
Beef: https://www.bqa.org/Media/BQA/Docs/nationalmanual.
pdf
Pork: http://www.pork.org/

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 23
3. Environmental Protection Agency (EPA) website for green
chemistry
www.epa.gov/greenchemistry

4. Safety and Data Sheets (SDS)

www.flinnsci.com/msds-search.aspx
A directory of SDS sheets from Flinn Scientific Inc. that includes a
ranking of hazard level and disposal methods.

www.ilpi.com/msds/index.html - A listing of numerous sites that

have free downloads of MSDS sheets.

5. Pesticides
National Pesticide Information Center
http://npic.orst.edu/ingred/products.html
Describes the various types of pesticides and the legal
requirements for labelling. Provides links and phone numbers to
get additional information.

Environmental Protection Agency

http://iaspub.epa.gov/apex/pesticides/f?p=PPLS:1
A database of product labels. Enter the product name or company
name to view the approved label information of pesticides which
are registered with the agency.

6. DEA Controlled Substances

Drug Enforcement Agency website:
https://www.dea.gov
Controlled Substance Schedules – a list of controlled substances:
www.deadiversion.usdoj.gov/schedules

7. Alcohol, Tobacco, Firearms, and Explosives

Alcohol and Tobacco Tax and Trade Bureau
www.ttb.gov
Bureau of Alcohol, Tobacco, Firearms and Explosives
www.atf.gov

8. Radiation
Radiation Studies Information (CDC)
www.cdc.gov/nceh/radiation/default.htm

9. CDC Laboratory Safety Manuals

www.cdc.gov/biosafety/publications/index.htm

10. Occupational Safety and Health Administration

www.osha.gov
Safety and Health Topics:
www.osha.gov/SLTC
www.osha.gov/SLTC/reactivechemicals/index.html
www.osha.gov/SLTC/laserhazards/index.html
www.osha.gov/SLTC/radiationionizing/index.html

11. U.S. Nuclear Regulatory Commission

Material Safety and Inspection Branch
One White Flint North
11555 Rockville Pike
Rockville, MD 20852
phone: 301-415-8200; 800-368-5642
www.nrc.gov

Page 24 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 ISEF Display & Safety Regulations
Please address any questions regarding ISEF Display & Safety Regulations to
[email protected]

Display & Safety Committee Mission

The mission of this committee is to ensure that all competitors qualify for competition according to the rules established in conjunction with the
Scientific Review Committee and Society for Science & the Public.

The ISEF Display & Safety inspection process can be initiated only when all items are present at the display. The Display & Safety Committee
Committee will offer guidance on Display & Safety issues for projects approved by the SRC to compete in ISEF. Occasionally, the ISEF Display
& Safety Committee may require students to make revisions to conform to Display & Safety regulations. Persistent issues will be directed to a
committee of individuals which may include Society for Science & the Public (SSP) personnel, Display & Safety (D & S) and/or Scientific Review
Committee (SRC) executive committee members.

The following regulations must be adhered to when a finalist exhibits a project at ISEF. All projects must adhere to the Display & Safety
requirements of the affiliated fair(s) in which they compete to qualify for participation in ISEF. Affiliated fairs may have additional restrictions or
requirements. Knowledge of these requirements is the responsibility of the Finalist, Adult Sponsor, and Fair Director.

Display Regulations Forms required at all projects:

1. An original Official Abstract and Certification as approved
Maximum Size of Project (stamped/embossed) by the ISEF Scientific Review Committee.
Depth (front to back): 30 inches or 76 centimeters a. Upon SRC approval, the stamped/embossed Official
Width (side to side): 48 inches or 122 centimeters Abstract and Certification will be provided.
Height (floor to top): 108 inches or 274 centimeters • The abstract must be the official International Science
and Engineering Fair Abstract and embossed/ stamped
Please be aware when ordering posters that the mechanism that
by the ISEF Scientific Review Committee.
supports the poster should conform to the maximum size limitations
• No other format or version of your approved Abstract
stated above.
& Certification will be allowed for any purpose at
1. All project materials and support mechanisms must fit within the
ISEF. Abstract handouts to judges and to the public
project dimensions (including table covers).
are limited to UNALTERED photocopies of the official
abstract and certification.
2. Fair-provided tables at ISEF will not exceed a height of 36 inches
b. The term “abstract” may NOT be used as a title or reference
(91 centimeters).
for any information on a finalist’s display or materials at the
project except as part of displaying the official stamped/
3. If a table is used it becomes part of the project and must not embossed abstract.
exceed the allowed dimensions. • It is the recommendation of the Display & Safety
Committee to NOT include the word “abstract” nor the
4. Nothing can be attached to the rear curtain. abstract itself when preparing backboards or posters
prior to the fair. However, it is reasonable to leave a
5. All demonstrations must be done within the confines of the blank space (8 ½” x 11”) on the backboard/poster so as
finalist’s booth space. When not being demonstrated, all project to facilitate the addition of the official abstract. Keep in
components must be returned to the project display and must fit mind this document can also be displayed vertically on
within allowable dimensions as defined above. the front edge of the table or in a free-standing acrylic
frame.
6. Projects can be continued under the table BUT this area is not to
be used for storage. 2. ISEF Project Set-up Approval Form (received on-site at the Fair)
Position of Project a. This form documents the project as approved by the Scientific
The fair provided table or freestanding display must be parallel to, and Review Committee and is used to document the Display &
positioned at, the back curtain of the booth. Projects may NOT lean Safety Committee’s review process and final approval.
against the back curtain. b. This form must be signed by the finalist and the Display &
Safety Committee member at the time of inspection.
Forms Required to be Visible and Vertically Displayed at Additional Forms required (only when applicable):
the Project Booth 1. Regulated Research Institutional/Industrial Setting Form (1C)
The placement of the required forms may include the front edge of a. If work was conducted in a regulated research institution,
the table, the display board, or in a free-standing acrylic frame placed industrial setting or any work site other than home, school
on the table top. or field at any time during the current ISEF project year,
the Regulated Research Institutional/Industrial Setting
Form (1C) must be completed and vertically displayed at the
project booth.
b. The information provided by the mentor on Form 1C may be
referenced to confirm that the information provided on the
project board is that of the finalist. Only minimal reference
to a mentor’s or another researcher’s work is allowable and
must only reflect background information or be used to
clarify differences between finalist’s and others’ work.

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 25
2. Continuation/Research Progression Projects Form (7) & Safety inspection. Examples of presentations that require
a. If a study is a continuation/research progression, the approval include, but are not limited to PowerPoint, Prezi,
Continuation/Research Progression Projects Form (7) must Keynote, software program/simulation and other images and/
be completed and vertically displayed at the project booth. or graphics displayed on a computer screen or other non-print
b. The display board and abstract must reflect only the current delivery method.
year’s work. The project title displayed in the finalist’s booth
may mention years of continuing research (for example,
“Year Two of an Ongoing Study”).
Items/Materials Not Allowed on Display or at Project
c. Reference to past work on the display board must be limited Booth
to summative past conclusory data and its comparison to 1. Any information on the project display or items that are
the current year data set. No raw data from previous years acknowledgments, self-promotions or external endorsements
may be publicly displayed; however, it may be included in are not allowed in the project booth.
the student research notebooks and/or logbooks if properly a. The use of logos including known commercial brands,
labeled. institutional crests or trademarks, flags unless integral to
the project and approved by the SRC via inclusion in the
Official Abstract and Certification.
Forms Required at Project but not Displayed
b. Personalized graphic/logos that are developed to indicate
1. Forms, excluding those listed above, that were required for the
a commercial purpose or viability of an established or
Scientific Review Committee approval should not be vertically
proposed business associated with the project. The only
displayed, but must be available in the booth in case asked for
exception is a student-created logo may be displayed at the
by a judge or other ISEF official. These forms include, but are
project once.
not limited to, Checklist for Adult Sponsor (1), Student Checklist
c. Any reference to an institution or mentor that supported
(1A), Research Plan, Approval Form (1B), and a photograph/video
the finalist’s research except as provided in the official ISEF
release form.
paperwork, most notably Form 1C.
d. Any reference to patent status of the project.
2. A photograph/video release form signed by the subject is required e. Any items intended for distribution such as disks, CDs, flash
for visual images of humans (other than the finalist) displayed as drives, brochures, booklets, endorsements, give-away items,
part of the project. business cards, printed materials or food items designed to
be distributed to judges or the public. Once again, handouts
Forms NOT to be at the Project Display Booth or in the to judges and to the public are limited to UNALTERED
Exhibit Hall photocopies of the official abstract and certification.
Completed informed consent/assent forms for a human participant
study are NOT to be displayed and should NOT be present at the 2. Any awards or medals, except for past or present ISEF medals that
project display. The Finalist may include a sample (incomplete) form in may be worn by the finalist.
their logbook or research notebook but under NO CIRCUMSTANCE
should the completed informed consent/assent forms for a human 3. Postal addresses, World Wide Web, email and/or social media
participant be in the Exhibit Hall. addresses, QR codes, telephone and/or fax numbers of a project
or finalist. Note: The only personal information that is permissible
Photograph/Image Display Requirements to include on the display is information that is also included on the
1. Any photograph/visual image/chart/table and/or graph is allowed Official Abstract and Certification (Finalist Name, School, City,
if: State, Country). Information regarding finalist’s age and grade are
a. It is not deemed offensive or inappropriate (which includes not permitted.
images/photographs showing invertebrate or vertebrate
animals/humans in surgical, necrotizing or dissection 4. Active Internet or email connections as part of displaying or
situations) by the Scientific Review Committee, the Display operating the project at ISEF.
& Safety Committee, or Society for Science & the Public.
b. It has a credit line of origin (“Photograph taken by...” or 5. Any changes, modifications, or additions to projects including any
“Image taken from...” or “Graph/Chart/Table taken from…”). attempt to uncover, replenish or return removed language or items
If all images, etc. displayed were created by the finalist or after the approval by the Display & Safety Committee and the
are from the same source, one credit line prominently and Scientific Review Committee has been received is prohibited.
vertically displayed on the backboard/poster or tabletop is a. Display & Safety inspections will include recording
sufficient. All images MUST BE properly cited. This includes photographic evidence of the approved Project Display and
background graphics, photographs and/or visual depictions Project booth.
of the finalist or photographs and/or visual depictions of b. Finalists who do not adhere to this signed agreement on the
others for which a signed photo/video release form is in ISEF Project Set-up Approval Form regarding this regulation
a notebook or logbook at the project booth. These signed may fail to qualify for competition. I/we understand that the
release forms must be available upon request during the set- initial Display & Safety Inspection has been completed, but
up and inspection process, but may not be displayed that additional reviews occur and that I/we should check
c. Sample release text: “I consent to the use of visual images back regularly. I/we will vertically display this signed form
(photos, videos, etc.) involving my participation/my child’s at our project at all times. I/we have not and will not store
participation in this research.” packing material under the booth. I/we further understand
that returning items that have been removed by the SRC or
2. Finalists using any presentation or demonstration outside of a D&S and/or adding items that are not permitted after final
project board must be prepared to show the entire presentation clearance are grounds for failing to qualify for competition
to the Display & Safety Inspectors before the project is approved. and/or forfeiture of all awards received.
All aforementioned rules apply to this presentation and the
presentation may not be altered in any way after the final Display

Page 26 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
Safety Regulations Electrical Regulations
1. Electrical power supplied to the project is 120 or 220 Volt, AC,
Not Allowed at Project or Booth
single phase, 60 Hz. No multi-phase will be available or shall be
Note: In the case in which a Finalist’s Project includes an item that is
used. Maximum circuit amperage/wattage available is determined
prohibited from display, please consider taking photographs and/or
by the electrical circuit capacities of the exhibit hall and may
documenting the significance of the prohibited item through video.
be adjusted on-site by the Display & Safety Committee. For all
1. Living organisms, including plants
electrical regulations, “120 Volt AC” or “220 Volt AC” is intended
to encompass the corresponding range of voltage as supplied by
2. Glass
the facility in which ISEF is being held.
3. Soil, sand, rock, cement and/or waste samples, even if permanently
2. Electrical devices must be protectively enclosed. Any enclosure
encased in a slab of acrylic
must be non-combustible. All external non-current carrying metal
parts must be grounded.
4. Taxidermy specimens or parts
3. Energized wiring, switches, and metal parts must have adequate
5. Preserved vertebrate or invertebrate animals
insulation and over-current safety devices (such as fuses) and must
be inaccessible to anyone other than the finalist. Exposed electrical
6. Human or animal food
equipment or metal that may be energized must be shielded with a
non-conducting material or with a grounded metal box to prevent
7. Human/animal parts or body fluids (for example, blood, urine)
accidental contact.
8. Plant materials (living, dead, or preserved) that are in their raw,
4. Decorative lighting or illumination is discouraged. If used, lighting
unprocessed, or non-manufactured state
must be as low a voltage as possible and must be LED lighting that
does not generate heat. Incandescent and fluorescent light bulbs
9. All chemicals including water. Absolutely no liquids can be utilized
are prohibited. When student is not at the exhibit, all electrical
in the Project Display
power must be disconnected, or power bars must be switched
off (Exception: during pre-judging audio visual displays may be
10. All hazardous substances or devices (Example: poisons, drugs,
available.)
firearms, weapons, ammunition, reloading devices, grease/oil and
sublimating solids such as dry ice)
5. An insulating grommet is required at the point where any wire or
cable enters any enclosure.
11. Items that may have contained or been in contact with hazardous
chemicals (Exception: Item may be permitted if professionally
6. No exposed live circuits over 36 volts are allowed.
cleaned and documentation for such cleaning is available). Filters
(including microbial) may not be displayed unless the Display
7. There must be an accessible, clearly visible on/off switch or other
& Safety Committee can reasonably determine that the device
means of quickly disconnecting from the 120 or 220 Volt power
was cleaned or was never used (please include receipts in your
source.
notebooks and/or logbooks)

12. Sharp items (for example, syringes, needles, pipettes, knives) Laser/Laser Pointer Regulations
Any Class 1, Class 2, Class 3A, or Class 3R lasers are allowed to be
13. Flames and highly flammable materials used responsibly. No other lasers may be used or displayed.
1. Laser beams may not pass through magnifying optics such as
14. Batteries with open-top cells or wet cells microscopes and telescopes. Lasers must be labeled by the
manufacturer so that power output can be inspected. Lasers
15. Drones or any flight-capable apparatus unless the propulsion without labels will NOT be permitted.
power source removed.
2. Lasers must be labeled by the manufacturer so that power output
16. 3D Printers unless the power source is removed. can be inspected. Lasers without labels will NOT be permitted.

17. Inadequately insulated apparatus capable of producing dangerous 3. Handheld lasers are NOT permitted.
temperatures are not permitted
4. Lasers will be confiscated with no warning if not used in a safe
18. Any apparatus with belts, pulleys, chains, or moving parts with manner.
tension or pinch points that are not appropriately shielded

19. Any display items that are deemed distracting (i.e. sounds, lights,
odors, etc.)

20. Personal items or packaging materials stored underneath the

booth

21. Any apparatus or project material deemed unsafe by the Scientific

Review Committee, the Display & Safety Committee, or the
Society

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 27
 ISEF Categories and Subcategories
The categories have been established with the goal of better aligning judges and student projects for the judging at ISEF. Local,
regional, state and country fairs may or may not choose to use these categories, dependent on the needs of their area. Please
check with your affiliated fair(s) for the appropriate category listings at that level of competition.

Please visit our website at student.societyforscience.org/intel-isef-categories-and-subcategories for a full description and

definition of ISEF categories:
ANIMAL SCIENCES (ANIM) CHEMISTRY (CHEM) ENERGY: PHYSICAL (EGPH) PHYSICS AND ASTRONOMY
Animal Behavior Analytical Chemistry Hydro Power (PHYS)
Cellular Studies Computational Chemistry Nuclear Power Astronomy and Cosmology
Development Environmental Chemistry Solar Atomic, Molecular, and Optical
Ecology Inorganic Chemistry Sustainable Design Physics
Genetics Materials Chemistry Thermal Power Biological Physics
Nutrition and Growth Organic Chemistry Wind Condensed Matter and Materials
Physiology Physical Chemistry Other Mechanics
Systematics and Evolution Other Nuclear and Particle Physics
Other ENGINEERING MECHANICS Theoretical, Computational and
COMPUTATIONAL BIOLOGY (ENMC) Quantum Physics
BEHAVIORAL AND SOCIAL AND BIOINFORMATICS Aerospace and Aeronautical Other
SCIENCES (BEHA) (CBIO) Engineering
Clinical and Developmental Computational Biomodeling Civil Engineering PLANT SCIENCES (PLNT)
Psychology Computational Epidemiology Computational Mechanics Agriculture and Agronomy
Cognitive Psychology Computational Evolutionary Control Theory Ecology
Neuroscience Biology Ground Vehicle Systems Genetics/Breeding
Physiological Psychology Computational Neuroscience Industrial Engineering-Processing Growth and Development
Sociology and Social Psychology Computational Pharmacology Mechanical Engineering Pathology
Other Genomics Naval Systems Plant Physiology
Other Other Systematics and Evolution
BIOCHEMISTRY (BCHM) Other
Analytical Biochemistry EARTH AND ENVIRONMENTAL
General Biochemistry ENVIRONMENTAL ENGINEERING (ENEV) ROBOTICS AND INTELLIGENT
Medical Biochemistry SCIENCES (EAEV) Bioremediation MACHINES (ROBO)
Structural Biochemistry Atmospheric Science Land Reclamation Biomechanics
Other Climate Science Pollution Control Cognitive Systems
Environmental Effects on Recycling and Waste Management Control Theory
BIOMEDICAL AND HEALTH Ecosystems Water Resources Management Machine Learning
SCIENCES (BMED) Geosciences Other Robot Kinematics
Cell, Organ, and Systems Water Science Other
Physiology Other MATERIALS SCIENCE (MATS)
Genetics and Molecular Biology of Biomaterials SYSTEMS SOFTWARE (SOFT)
Disease EMBEDDED SYSTEMS (EBED) Ceramic and Glasses Algorithms
Immunology Circuits Composite Materials Cybersecurity
Nutrition and Natural Products Internet of Things Computation and Theory Databases
Pathophysiology Microcontrollers Electronic, Optical and Magnetic Human/Machine Interface
Other Networking and Data Materials Languages and Operating Systems
Communications Nanomaterials Mobile Apps
BIOMEDICAL ENGINEERING Optics Polymers Online Learning
(ENBM) Sensors Other Other
Biomaterials and Regen Medicine Signal Processing
Biomechanics Other MATHEMATICS (MATH) TRANSLATIONAL MEDICAL
Biomedical Devices Analysis SCIENCES (TMED)
Biomedical Imaging ENERGY: CHEMICAL (EGCH) Combinatorics, Graph Theory, and Disease Detection and Diagnosis
Cell and Tissue Engineering Alternative Fuels Game Theory Disease Prevention
Synthetic Biology Computational Energy Science Geometry and Topology Disease Treatment and Therapies
Other Fossil Fuel Energy Number Theory Drug Identification and Testing
Fuel Cells and Battery Develop Probability and Statistics Pre-Clinical Studies
CELLULAR AND MOLECULAR Microbial Fuel Cells Other Other
BIOLOGY (CELL) Solar Materials
Cell Physiology Other MICROBIOLOGY (MCRO)
Antimicrobials and Antibiotics
Cellular Immunology
Genetics Applied Microbiology
Molecular Biology Bacteriology
Neurobiology Environmental Microbiology
Other Microbial Genetics
Virology
Other

Page 28 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Information on Required Abstract & Certification for ALL Projects at ISEF
* This form may not be relevant for your regional or state fair; please refer to instructions from your affiliated fair.*

In ADDITION to the basic form requirements for ALL Projects and any other requirements due to specific areas of research,
an Abstract & Certification is required at the conclusion of research. Details on this requirement follow.

Completing the Abstract ISEF Sample Abstract & Certification

After finishing research and experimentation,
you are required to write a (maximum) 250 Project Title Project ID
word, one-page abstract. For ISEF, this abstract
is written in the online Finalist Questionnaire Finalist Name(s)
Category
portal and submitted electronically. Pick one only-- mark an “X” in
Finalist School, City, State/Province, Country box at right

It is recommended that it include the

following: Abstract Body
a. purpose of the experiment
b. procedure
c. data
d. conclusions

It may also include any possible research

applications. Only minimal reference to
previous work may be included.

An abstract must not include the following:

a. acknowledgments (including naming the
research institution and/or mentor with which
you were working), or self-promotions and
external endorsements 1. As a part of this research project, the student directly handled, manipulated, or
b. logos or proper names of commercial products interacted with (check all that apply):
b. work or procedures done by the mentor
o human participants o potentially hazardous biological agents
Completing the Certification o vertebrate animals o microorganisms o rDNA o tissue

At the bottom of the Abstract & Certification

2. This abstract describes only procedures performed by me/us, reflects my/our
form there are six questions. Please read each own independent research, and represents one year’s work only.
carefully and answer appropriately. The ISEF o yes o no
Scientific Research Committee will review
and approve the abstract and answers to the 3. I/We worked or used equipment in a regulated research institution or industrial
questions. setting.
o yes o no
Revisions are permitted via the online portal
through late April (please reference the system
for current year deadlines.) 4. This project is a continuation of previous research.
o yes o no
Once approved, two copies of the ISEF
Abstract & Certification will be provided with
a gold embossed seal; only this version of the 5. My display board includes non-published photographs/visual depictions of
abstract may be displayed or distributed. humans (other than myself):
o yes o no

6. I/We hereby certify that the abstract and responses to the above statements are
NOTE: Your abstract must be on the correct and properly reflect my/our own work.
International Science and Engineering Fair o yes o no
Abstract & Certification form and have the
ISEF Scientific Review Committee approval
seal before it is displayed or handed out. No
other format or version of your approved
Abstract will be allowed for any purpose at the
FOR ISEF
ISEF. OFFICIAL USE
ONLY

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 29
 Checklist for Adult Sponsor (1)
This completed form is required for ALL projects.

To be completed by the Adult Sponsor in collaboration with the student researcher(s):

Student’s Name(s):
Project Title:
1. o
✔ I have reviewed the ISEF Rules and Guidelines.

2. o I
✔ have reviewed the student’s completed Student Checklist (1A) and Research Plan/Project Summary.

3. o I
✔ have worked with the student and we have discussed the possible risks involved in the project.

4. o The project involves one or more of the following and requires prior approval by an SRC, IRB, IACUC or IBC:
o Humans Potentially Hazardous Biological Agents
o Vertebrate Animals o Microorganisms o rDNA o Tissues

5. o Items
✔ to be completed for ALL PROJECTS
o✔ Adult Sponsor Checklist (1) o✔ Research Plan/Project Summary
o✔ Student Checklist (1A) o Approval
✔ Form (1B)
o Regulated
✔ Research Institutional/Industrial Setting Form (1C) (when applicable; after completed experiment)
o Continuation/Research Progression Form (7) (when applicable)

Additional forms required if the project includes the use of one or more of the following (check all that apply):
†† Humans, including student designed inventions/prototypes. (Requires prior approval by an Institutional Review Board (IRB);
see full text of the rules.)
†† Human Participants Form (4) or appropriate Institutional IRB documentation
†† Sample of Informed Consent Form (when applicable and/or required by the IRB)
†† Qualified Scientist Form (2) (when applicable and/or required by the IRB)

†† Vertebrate Animals (Requires prior approval, see full text of the rules.)
†† Vertebrate Animal Form (5A) ‑ for projects conducted in a school/home/field research site (SRC prior approval required.)
†† Vertebrate Animal Form (5B) ‑ for projects conducted at a Regulated Research Institution. (Institutional Animal Care and Use
Committee (IACUC) approval required prior experimentation.)
†† Qualified Scientist Form (2) (Required for all vertebrate animal projects at a regulated research site or when applicable)

†† Potentially Hazardous Biological Agents (Requires prior approval by SRC, IACUC or IBC, see full text of the rules.)
†† Potentially Hazardous Biological Agents Risk Assessment Form (6A)
†† Human and Vertebrate Animal Tissue Form (6B) ‑ to be completed in addition to Form 6A when project involves the use of fresh or
frozen tissue, primary cell cultures, blood, blood products and body fluids.
†† Qualified Scientist Form (2) (when applicable)
†† The following are exempt from prior review but require a Risk Assessment Form 3: projects involving protists, archae and similar
microorganisms, for projects using manure for composting, fuel production or other non-culturing experiments, projects using color
change coliform water test kits, microbial fuel cells, and projects involving decomposing vertebrate organisms.

†† Hazardous Chemicals, Activities and Devices (No SRC prior approval required, see full text of the rules.)
†† Risk Assessment Form (3)
†† Qualified Scientist Form (2) (required for projects involving DEA-controlled substances or when applicable)

†
✔
† Other
✔ †
† Risk Assessment Form (3)

Adult Sponsor’s Printed Name Signature Date of Review (mm/dd/yy)

[email protected]

Phone Email
Page 30 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Student Checklist (1A)
This form is required for ALL projects.

1. a. Student/Team Leader: Grade:

Email: Phone:

b. Team Member: c. Team Member: ______________________________

2. Title of Project:

3. School: School Phone:

School Address:

4.
Adult Sponsor: Phone/Email:

5. Does this project need SRC/IRB/IACUC or other pre-approval? o Yes o No Tentative start date: ___________ ✔

6. Is this a continuation/progression from a previous year? o Yes o No ✔

If Yes:
a. Attach the previous year’s † Abstract and o Research Plan/Project Summary
b. Explain how this project is new and different from previous years on
† Continuation/Research Progression Form (7)

7. This year’s laboratory experiment/data collection:

Actual Start Date: (mm/dd/yy) End Date: (mm/dd/yy)

8. Where will you conduct your experimentation? (check all that apply)
o
Research Institution
✔
o School
✔
o Field o Home
✔
o Other: ________________________

9. List name and address of all non-home and non-school work site(s):
Name:

Address:

Phone/
email

10. Complete a Research Plan/Project Summary following the Research Plan/Project Summary instructions
and attach to this form.

11. An abstract is required for all projects after experimentation.

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 31
 Research Plan/Project Summary Instructions
A complete Research Plan/Project Summary is required for ALL projects and
must accompany Student Checklist (1A).

1. All projects must have a Research Plan/Project Summary

a. Written prior to experimentation following the instructions below to detail the rationale, research question(s), methodology, and risk
assessment of the proposed research.
b. If changes are made during the research, such changes can be added to the original research plan as an addendum, recognizing that
some changes may require returning to the IRB or SRC for appropriate review and approvals. If no additional approvals are required,
this addendum serves as a project summary to explain research that was conducted.
c. If no changes are made from the original research plan, no project summary is required.

2. Some studies, such as an engineering design or mathematics projects, will be less detailed in the initial project plan and will change through
the course of research. If such changes occur, a project summary that explains what was done is required and can be appended to the original
research plan.

3. The Research Plan/Project Summary should include the following:

a. RATIONALE: Include a brief synopsis of the background that supports your research problem and explain why this research is
important and if applicable, explain any societal impact of your research.
b. RESEARCH QUESTION(S), HYPOTHESIS(ES), ENGINEERING GOAL(S), EXPECTED OUTCOMES: How is this based on the rationale
described above?
c. Describe the following in detail:
• Procedures: Detail all procedures and experimental design including methods for data collection. Describe only your project. Do not include
work done by mentor or others.
• Risk and Safety: Identify any potential risks and safety precautions needed.
• Data Analysis: Describe the procedures you will use to analyze the data/results.
d. BIBLIOGRAPHY: List major references (e.g. science journal articles, books, internet sites) from your literature review. If you plan to use
vertebrate animals, one of these references must be an animal care reference.

Items 1–4 below are subject-specific guidelines for additional items to be included in your research plan/project summary as applicable.
1. Human participants research:
a. Participants: Describe age range, gender, racial/ethnic composition of participants. Identify vulnerable populations (minors, pregnant
women, prisoners, mentally disabled or economically disadvantaged).
b. Recruitment: Where will you find your participants? How will they be invited to participate?
c. Methods: What will participants be asked to do? Will you use any surveys, questionnaires or tests? If yes and not your own, how did
you obtain? Did it require permissions? If so, explain. What is the frequency and length of time involved for each subject?
d. Risk Assessment: What are the risks or potential discomforts (physical, psychological, time involved, social, legal, etc.) to participants?
How will you minimize risks? List any benefits to society or participants.
e. Protection of Privacy: Will identifiable information (e.g., names, telephone numbers, birth dates, email addresses) be collected? Will
data be confidential/anonymous? If anonymous, describe how the data will be collected. If not anonymous, what procedures are in
place for safeguarding confidentiality? Where will data be stored? Who will have access to the data? What will you do with the data
after the study?
f. Informed Consent Process: Describe how you will inform participants about the purpose of the study, what they will be asked to do,
that their participation is voluntary and they have the right to stop at any time.

2. Vertebrate animal research:

a. Discuss potential ALTERNATIVES to vertebrate animal use and present justification for use of vertebrates.
b. Explain potential impact or contribution of this research.
c. Detail all procedures to be used, including methods used to minimize potential discomfort, distress, pain and injury to the animals and
detailed chemical concentrations and drug dosages.
d. Detail animal numbers, species, strain, sex, age, source, etc., include justification of the numbers planned.
e. Describe housing and oversight of daily care.
f. Discuss disposition of the animals at the end of the study.

3. Potentially hazardous biological agents research:

a. Give source of the organism and describe BSL assessment process and BSL determination.
b. Detail safety precautions and discuss methods of disposal.

4. Hazardous chemicals, activities & devices:

• Describe Risk Assessment process, supervision, safety precautions and methods of disposal.
• Material Safety Data Sheets are not necessary to submit with paperwork.

Page 32 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Approval Form (1B)
A completed form is required for each student, including all team members.

1. To Be Completed by Student and Parent

a. Student Acknowledgment:
• I understand the risks and possible dangers to me of the proposed research plan.
• I have read the ISEF Rules and Guidelines and will adhere to all International Rules when conducting this
research.
• I have read and will abide by the following Ethics statement
Student researchers are expected to maintain the highest standards of honesty and integrity. Scientific fraud and misconduct
are not condoned at any level of research or competition. Such practices include but are not limited to plagiarism, forgery, use
or presentation of other researcher’s work as one’s own, and fabrication of data. Fraudulent projects will fail to qualify for
competition in affiliated fairs and ISEF.
Toni Samala

Student’s Printed Name Signature Date Acknowledged (mm/dd/yy)

(Must be prior to experimentation.)
b. Parent/Guardian Approval: I have read and understand the risks and possible dangers involved in the
Research Plan/Project Summary. I consent to my child participating in this research.
Francis Austria
Parent/Guardian’s Printed Name Signature Date Acknowledged (mm/dd/yy)
(Must be prior to experimentation.)

2. To be completed by the local or affiliated Fair SRC

(Required for projects requiring prior SRC/IRB APPROVAL. Sign 2a or 2b as appropriate.)

a. Required for projects that need prior SRC/IRB approval b. Required for research conducted at all Regulated Research
BEFORE experimentation (humans, vertebrates or potentially Institutions with no prior fair SRC/IRB approval.
hazardous biological agents). OR
This project was conducted at a regulated research institution
The SRC/IRB has carefully studied this project’s Research Plan/ (not home or high school, etc.), was reviewed and approved by the
Project Summary and all the required forms are included. My proper institutional board before experimentation and complies
signature indicates approval of the Research Plan/Project Summary with the ISEF Rules. Attach (1C) and any required institutional
before the student begins experimentation. approvals (e.g. IACUC, IRB).

SRC/IRB Chair’s Printed Name

SRC Chair’s Printed Name

Signature Date of Approval (mm/dd/yy)

Signature Date of Signature (mm/dd/yy)
(Must be prior to experimentation.) (May be after experimentation)

3. Final ISEF Affiliated Fair SRC Approval (Required for ALL Projects)

SRC Approval After Experimentation and Before Competition at Regional/State/National Fair

I certify that this project adheres to the approved Research Plan/Project Summary and complies with all ISEF Rules.

Regional SRC Chair’s Printed Name Signature Date of Approval (mm/dd/yy)

State/National SRC Chair’s Printed Name Signature Date of Approval (mm/dd/yy)

(where applicable)

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 33
 Regulated Research Institutional/Industrial Setting Form (1C)
This form must be completed AFTER experimentation by the adult supervising the student research conducted
in a regulated research institution, industrial setting or any work site other than home, school or field.

Student’s Name(s)

Title of Project
To be completed by the Supervising Adult in the Setting (NOT the Student(s)) after experimentation:
(Responses must be on the form as it is required to be displayed at student’s project booth; please do not print double-sided.)

The student(s) conducted research at my work site:

1. Did you or your proxy (e.g. graduate student, postdoc, employee) mentor or provide
substantial guidance to the student researcher? o Yes
✔
o No
a. If no, describe your and/or your institution’s role with the student researcher and
his/her project (e.g. supervised use of equipment on site without ongoing mentorship
and sign below.

b. If yes, complete questions 2 –5.

2. Is the student’s research project a subset of your ongoing research or work? o Yes o No
✔

Use questions 3, 4 and 5 to detail how the student’s project was similar and/or
different from ongoing research or work at your site.

3. Describe the independence and creativity with which the student:

a. developed the hypotheses or engineering goals for the research project

b. designed the methodology for his/her research project

c. analyzed and interpreted data

(Continued on next page)

Page 34 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Regulated Research Institutional/Industrial Setting Form (1C)
Continued

Student’s Name(s)

4. Detail the student’s role in conducting the research (e.g. data collection, specific procedures
performed). Differentiate what the student observed and what the student actually did.

5. Did the student(s) work on the project as part of a group? o

✔
Yes o No
If yes, how many individuals were in the group and who were they (e.g. high school
students, graduate students, faculty, professional researchers)?

I attest that the student has conducted the work as indicated above and that any required review and approval by
institutional regulatory board (IRB/IACUC/IBC) has been obtained. Copies are attached if applicable.
I further acknowledge that the student will be presenting this work publicly in competition and I have communicated with the
student research regarding any requirements for my review and/or restrictions of what is publicized.

Supervising Adult’s Printed Name Signature Title

Institution Date Signed (must be after experi-

mentation) (mm/dd/yy)

Address Email/Phone

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 35
 Qualified Scientist Form (2)
May be required for research involving human participants, vertebrate animals, potentially hazardous biological agents, and hazardous
substances and devices. Must be completed and signed before the start of student experimentation.

Student’s Name(s)

Title of Project

To be completed by the Qualified Scientist:

Scientist Name:
Educational Background: Degree(s):
Experience/Training as relates to the student’s area of
research:

Position: Institution:

Address: Email/Phone:

1. Have you reviewed the ISEF rules relevant to this project? o Yes
✔
o No

2. Will any of the following be used?

a. Human participants o Yes o No
✔

b. Vertebrate animals o Yes o No

✔

c. Potentially hazardous biological agents (microorganisms, rDNA and tissues,

including blood and blood products) o Yes o No
✔

d. Hazardous substances and devices o Yes o No

✔

3. Will this study be a sub-set of a larger study? o Yes o No

✔

4. Will you directly supervise the student? o Yes

✔
o No
a. If no, who will directly supervise and serve as the Designated Supervisor?
b. Experience/Training of the Designated Supervisor:

To be completed by the Qualified Scientist: To be completed by the Designated Supervisor

when the Qualified Scientist cannot directly supervise.
I certify that I have reviewed and approved the Research Plan/
Project Summary prior to the start of the experimentation. If the I certify that I have reviewed the Research Plan/Project Summary
student or Designated Supervisor is not trained in the necessary and have been trained in the techniques to be used by this student,
procedures, I will ensure her/his training. I will provide advice and and I will provide direct supervision.
supervision during the research. I have a working knowledge of the
techniques to be used by the student in the Research Plan/Project
Summary. I understand that a Designated Supervisor is required
when the student is not conducting experimentation under my Designated Supervisor’s Printed Name
direct supervision.

Signature Date of Approval (mm/dd/yy)

Qualified Scientist’s Printed Name

Signature Date of Approval (mm/dd/yy) Phone Email

Page 36 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Risk Assessment Form (3)
Must be completed before experimentation.

Student’s Name(s)
Title of Project

To be completed by the Student Researcher(s) in collaboration with Designated Supervisor/Qualified Scientist:

(All questions must be answered; additional page(s) may be attached.)

1. List all hazardous chemicals, activities, or devices that will be used; identify microorganisms exempt from pre-approval (see
Potentially Hazardous Biological Agent rules).

None

2. Identify and assess the risks involved in this project.

The risks include having erythema (burns) and physical cuts.

3. Describe the safety precautions and procedures that will be used to reduce the risks.

See page attached.

4. Describe the disposal procedures that will be used (when applicable).

Throwing in proper places

5. List the source(s) of safety information.

Health and Safety Literatures and DOST officers

To be completed and signed by the Designated Supervisor (or Qualified Scientist, when applicable):
I agree with the risk assessment and safety precautions and procedures described above. I certify that I have reviewed the Research
Plan/Project Summary and will provide direct supervision.

Designated Supervisor’s Printed Name Signature Date of Review (mm/dd/yy)

Position & Institution Phone or email contact information

Experience/Training as relates to the student’s area of research

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 37
 Human Participants Form (4)
Required for all research involving human participants not at a Regulated Research Institution. If at a Regulated Research Institution,
use institutional approval forms for documentation of prior review and approval.
(IRB approval required before recruitment or data collection.)

Student’s Name(s) Title of Project

Adult Sponsor Phone/Email

Must be completed by Student Researcher(s) in collaboration with the Adult Sponsor/Designated Supervisor/Qualified Scientist:
1. o I have submitted my Research Plan/Project Summary which addresses ALL areas indicated in the Human Participants Section of the
Research Plan/Project Summary Instructions.
2. I have attached any surveys or questionnaires I will be using in my project or other documents provided to human participants.
† Any published instrument(s) used was/were legally obtained.
3. I have attached an informed consent that I would use if required by the IRB.
4. o Yes o No Are you working with a Qualified Scientist? If yes, attach the Qualified Scientist Form 2.

BELOW  ‑  IRB USE ONLY

Must be completed by Institutional Review Board (IRB) after review of the research plan. All questions must be answered for the approval
to be valid. (If not approved, return paperwork to the student with instructions for modifications.)
o Approved with Full Committee Review (3 signatures required) and the following conditions: (All 6 must be answered)
1. Risk Level (check one) : o Minimal Risk o More than Minimal Risk
2. Qualified Scientist (QS) Required (Form 2): o Yes o No
3. Designated Supervisor (DS) Required (Form 3): o Yes o No
4. Written Minor Assent required for minor participants:
o Yes o No o Not applicable (No minors in this study)
5. Written Parental Permission required for minor participants:
o Yes o No o Not applicable (No minors in this study)
6. Written Informed Consent required for participants 18 years or older:
o Yes o No o Not applicable (No participants 18 yrs or older in this study)

IRB SIGNATURES (All 3 signatures required) None of these individuals may be the adult sponsor, designated supervisor, qualified scientist or
related to (e.g., mother, father of) the student (conflict of interest).
I attest that I have reviewed the student’s project, that the checkboxes above have been completed to indicate the IRB determination and
that I agree with the decisions above.

Medical or Mental Health Professional (a psychologist, medical doctor, licensed social worker, licensed clinical professional counselor, physician’s assistant,
doctor of pharmacy, or registered nurse) with expertise related to this project.

Printed Name Degree/Professional License

Signature Date of Approval (Must be prior to experimentation.) (mm/dd/yy)

Educator

Printed Name Degree/Professional License

Signature Date of Approval (Must be prior to experimentation.) (mm/dd/yy)

School Administrator

Printed Name Degree/Professional License

Signature Date of Approval (Must be prior to experimentation.) (mm/dd/yy)

Page 38 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Human Informed Consent Form

Instructions to the Student Researcher(s): An informed consent/assent/permission form should be developed in

consultation with the Adult Sponsor, Designated Supervisor or Qualified Scientist.
This form is used to provide information to the research participant (or parent/guardian) and to document written informed consent,
minor assent, and/or parental permission.
• When written documentation is required, the researcher keeps the original, signed form.
• Students may use this sample form or may copy ALL elements of it into a new document.

If the form is serving to document parental permission, a copy of any survey or questionnaire must be attached.

Student Researcher(s):
Title of Project:

I am asking for your voluntary participation in my science fair project. Please read the following information about the project. If you
would like to participate, please sign in the appropriate area below.

Purpose of the project:

If you participate, you will be asked to:

Time required for participation:

Potential Risks of Study:

Benefits:

How confidentiality will be maintained:

If you have any questions about this study, feel free to contact:

Adult Sponsor/QS/DS: ____________________________________ Phone/email: ____________________

Voluntary Participation:
Participation in this study is completely voluntary. If you decide not to participate there will not be negative consequences. Please
be aware that if you decide to participate, you may stop participating at any time and you may decide not to answer any specific
question.

By signing this form I am attesting that I have read and understand the information above and I freely give my consent/assent to
participate or permission for my child to participate.

Adult Informed Consent or Minor Assent Date Reviewed & Signed:

(mm/dd/yy)

Research Participant Printed Name: Signature:

Parental/Guardian Permission (if applicable) Date Reviewed & Signed:

(mm/dd/yy)

Parent/Guardian Printed Name: Signature:

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 39
 Vertebrate Animal Form (5A)
Required for all research involving vertebrate animals that is conducted in a school/home/field research site.
(SRC approval required before experimentation.)

Student’s Name(s)

Title of Project

To be completed by Student Researcher:

1. Common name (or Genus, species) and number of animals used.

2. Describe completely the housing and husbandry to be provided. Include the cage/pen size, number of animals per cage,
environment, bedding, type of food, frequency of food and water, how often animal is observed, etc. Add an additional
page as necessary.

3. What will happen to the animals after experimentation?

4. Attach a copy of wildlife licenses or approval forms, as applicable

5. The ISEF Vertebrate Animal Rules require that any death, illness or unexpected weight loss be investigated and
documented by a letter from the qualified scientist, designated supervisor or a veterinarian. If applicable, attach this letter
with this form when submitting your paperwork to the SRC prior to competition.

To be completed by Local or Affiliate Fair Scientific Review Committee (SRC) BEFORE experimentation.
Level of Supervision Required for agricultural, behavioral or nutritional studies (select one):
o Designated Supervisor REQUIRED. Please have applicable person sign below.
o Veterinarian and Designated Supervisor REQUIRED. Please have applicable persons sign below.
o
Veterinarian, Designated Supervisor and Qualified Scientist REQUIRED. Please have applicable persons sign below and have the Qualified
Scientist complete Form (2).
The SRC has carefully reviewed this study and finds it is an appropriate study that may be conducted in a non-regulated research site.
Local or Affiliate Fair SRC Pre-Approval Signature:

SRC Chair Printed Name Signature Date of Approval (must be prior to experimentation)
(mm/dd/yy)

To be completed by Veterinarian: To be completed by Designated Supervisor or Qualified

Scientist when applicable:
o I have reviewed this research and animal husbandry with the
student before the start of experimentation. o I have reviewed this research and animal husbandry with
the student before the start of experimentation and I accept
o I have approved the use and dosages of prescription drugs and/ primary responsibility for the care and handling of the
or nutritional supplements. animals in this project.
o I will provide veterinary medical and nursing care in case of o I will directly supervise the experiment.
illness or emergency. (Fees may apply.)

Printed Name Email/Phone Printed Name Email/Phone

Signature Date of Approval (mm/dd/yy) Signature Date of Approval (mm/dd/yy)

Page 40 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Vertebrate Animal Form (5B)
Required for all research involving vertebrate animals that is conducted in at a Regulated Research Institution.
(IACUC approval required before experimentation. Form must be completed and signed after experimentation.)

Student’s Name(s)

Title of Project
Title and Protocol Number of IACUC Approved Project

To be completed by Qualified Scientist or Principal Investigator:

1. Species of animals used: Number of animals used:

2. Describe, in detail, the role of the student in this project: animal procedures and related equipment that were
involved, oversight provided and safety precautions employed. (Attach extra pages if necessary.)

3. Was there any weight loss or death of any animal? If yes, attach a letter obtained from the qualified scientist,
designated supervisor or a veterinarian documenting the situation and the results of the investigation.

4. Did the student’s project also involve the use of tissues?

p No
p Yes; complete Forms 6A and 6B

5. What laboratory training, including dates, was provided to the student?

6. Attach a copy of the Regulated Research Institution IACUC Approval. A letter from the Qualified Scientist or
Principal Investigator is not sufficient.

Qualified Scientist/Principal Investigator

Printed Name

Signature Date (mm/dd/yy)

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 41
 Potentially Hazardous Biological Agents Risk Assessment Form (6A)
Required for research involving microorganisms, rDNA, fresh/frozen tissue (including primary cell lines, human and
other primate established cell lines and tissue cultures), blood, blood products and body fluids.
SRC/IACUC/IBC approval required before experimentation.

Student’s Name(s)

Title of Project
To be completed by the QUALIFIED SCIENTIST/DESIGNATED SUPERVISOR in collaboration with the student researcher(s). All
questions are applicable and must be answered; additional page(s) may be attached.
SECTION 1: PROJECT ASSESSMENT
1. Identify potentially hazardous biological agents to be used in this experiment. Include the source, quantity and the biosafety level risk
group of each microorganism.

2. Describe the site of experimentation including the level of biological containment.

3. Describe the procedures that will be used to minimize risk (personal protective equipment, hood type, etc.).

4. What final biosafety level do you recommend for this project given the risk assessment you conducted?

5. Describe the method of disposal of all cultured materials and other potentially hazardous biological agents.

SECTION 2: TRAINING
1. What training will the student receive for this project?

2. Experience/training of Designated Supervisor as it relates to the student’s area of research (if applicable).

SECTION 3: For ALL CELL LINES, MICROORGANISMS AND TISSUES – To be completed by the QUALIFIED SCIENTIST or
DESIGNATED SUPERVISOR - Check the appropriate box(es) below:
o Experimentation on the microorganisms/cell lines/tissues to be used in this study will NOT be conducted at a Regulated Research Institution, but will
be conducted at a (check one) ___BSL-1 or ___BSL-2 laboratory. This study has been reviewed by the local SRC and the procedures have been approved
prior to experimentation.

o Experimentation on the microorganisms/cell lines/tissues to be used in this study will be conducted at a Regulated Research Institution and was
approved by the appropriate institutional board prior to experimentation; institutional approval forms are attached.
Origin of cell lines:__________________________________________ Date of IACUC/IBC approval ___________________________

o Experimentation on the microorganisms/cell lines/tissues to be used in this study will be conducted at a Regulated Research Institution, which does
not require pre-approval for this type of study. The SRC has reviewed that the student received appropriate training and the project complies with ISEF
rules.

CERTIFICATION – To be SIGNED by the QUALIFIED SCIENTIST or DESIGNATED SUPERVISOR

The QS/DS has seen this project’s research plan and supporting documentation and acknowledges the accuracy of the information provided
above. This study has been approved as a (check one) o BSL-1/ o  
BSL-2 study, and will be conducted in an appropriate laboratory.

QS/DS Printed Name Signature

Date of review (mm/dd/yy)

SECTION 4: CERTIFICATION – To be completed by the LOCAL or AFFILIATED FAIR SRC

The SRC has seen this project’s research plan and supporting documentation and acknowledges the accuracy of the information provided above.

SRC Printed Name Signature

Date of review (mm/dd/yy)

Page 42 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
 Human and Vertebrate Animal Tissue Form (6B)
Required for research involving fresh/frozen tissue (including primary cell lines, human and other primate established cell lines and tissue
cultures), blood, blood products and body fluids. If the research involves living organisms please ensure that the proper human or animal
forms are completed. All projects using any tissue listed above must also complete Form 6A.

Student’s Name(s)

Title of Project

To be completed by Student Researcher(s):

1. What vertebrate animal tissue will be used in this study? Check all that apply.
o Fresh or frozen tissue sample
o Fresh organ or other body part
o Blood
o Body fluids
o Primary cell/tissue cultures
o Human or other primate established cell lines

2. Where will the above tissue(s) be obtained. If using an established cell line include source and catalog number.

3. If the tissue will be obtained from a vertebrate animal study conducted at a research institution attach a copy of the IACUC
certification with the name of the research institution, the title of the study, the IACUC approval number and a of IACUC
approval.

To be completed by the Qualified Scientist or Designated Supervisor:

o I verify that the student will work solely with organs, tissues, cultures or cells that will be supplied to him/her by myself or qualified
personnel from the laboratory; and that if vertebrate animals were euthanized they were euthanized for a purpose other than the
student’s research.
AND/OR
o I certify that the blood, blood products, tissues or body fluids in this project will be handled in accordance with the standards and
guidance set forth in U.S. Occupational Safety and Health Act, 29CFR, Subpart Z, 1910.1030 - Blood Borne Pathogens.

Printed Name Signature Date of Approval (mm/dd/yy)

(Must be prior to experimentation.)

Title Phone/Email

Institution

International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 43
 Continuation/Research Progression Projects Form (7)
Required for projects that are a continuation/progression in the same field of study as a previous project.
This form must be accompanied by the previous year’s abstract and Research Plan/Project Summary.

Student’s Name(s)

To be completed by Student Researcher: List all components of the current project that make it new and different from previous research.
The information must be on the form; use an additional form for previous year and earlier projects.

Components Current Research Project Previous Research Project: Year: ________

1. Title

2. Change in goal/
purpose/objective

3. Changes in
methodology

4. Variable studied

5. Additional
changes

Attached are:
o Abstract and Research Plan/Project Summary, Year _________

I hereby certify that the above information is correct and that the current year Abstract & Certification and project display board
properly reflect work done only in the current year.

Student’s Printed Name(s) Signature Date of Signature (mm/dd/yy)

Page 44 International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020
International Rules: Guidelines for Science and Engineering Fairs 2019 – 2020, societyforscience.org/ISEF2020 Page 45
The International Science and Engineering Fair encourages students to tackle challenging scientific
questions and develop the skills needed to solve the problems of tomorrow.

Society for Science & the Public

The Society for Science & the Public is a champion for science, dedicated to expanding scientific literacy,
effective STEM education and scientific research. Founded in 1921, we are a nonprofit 501(c)(3)
membership organization focused on promoting the understanding and appreciation of science and the
vital role it plays in human advancement. Through its acclaimed science research competitions, including
the Regeneron Science Talent Search, the International Science and Engineering Fair and the Broadcom
MASTERS, and its award-winning magazine, Science News and digital media properties, Science News for
Students, the Society is committed to inform, educate and inspire.

Learn more at:

www.societyforscience.org
and follow us on
Facebook https://www.facebook.com/societyforscience
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Society for Science & the Public

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Washington, DC 20036-2801
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societyforscience.org/ISEF2020

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2019 Society for Science & the Public. All rights reserved. International Science and Engineering
Fair is registered in the U.S. Patent and Trademark Office as a trademark of Society for Science & the
Public. *Other names and brands may be claimed as the property of others. Printed in USA Please
Recycle.