THE LEADING CHOICE IN

HPLC Consumables
Proven Quality, Reliability and Results!
2012-2013 HPLC Consumable Catalogue
ABOUT US
伯森生物科技股份有限公司成立於 1998 年 7 月，
是由一群熱情於工作的夥伴所組成，成員們多數來
自國內生命科學領域的碩士班畢業生，並經過嚴格
的訓練，對於工作始終抱持著熱情、積極、專業及
絕對負責的態度。

伯森公司的成立宗旨是希望以零時差的速度將最新
的知識與技術產品引進國內，為提升國內生物技術
產業研發能量盡一份心力。目前伯森代理的廠牌包
含有：PerkinElmer、Enzo Life Sciences、HANNA
instruments、Wealtec、ibidi、Lucigen、Mirus Bio、
ScienCell、Advanced BioMatrix、Cellendes、
Biochain、Exiqon、Gene Tools、ProSci 等…多家國
際知名生命科學試劑、耗材與儀器廠商。至今伯森
仍持續藉由參加國際研討會及閱讀相關期刊，並經
過審慎專業的篩選與評估，為國內研究領域發掘出
更多優質的技術與產品。

您的建議是我們進步最大的動力與推手，若您有任
何寶貴意見或是任何關於伯森代理產品的疑問，竭
誠歡迎您隨時透過伯森業務專員或是以電話、電子
郵件的方式與我們聯絡，我們將衷心為您服務，謝
謝。
CHROMATOGRAPHY
PerkinElmer has been a leading provider of Chromatography Liquid Chromatography
solutions for over 50 years. Our team of dedicated Chromatog- Accessories - LC/MS 2-3
raphy Specialists has an expertise that boasts years of knowl-
Accessories and
edge in understanding a laboratory's product requirements. This Autosamplers - LC 4-6
knowledge allows us to give you a focused consumables Chromatography Data
portfolio to fit your applications. From colored inlet liners that Systems 7
help you easily identify the correct liner for your application to Vials, Caps and Septa 8-10
gas purification systems that remove up to 99.99% of contami-
Syringe Filters and
nants, our precisiondesigned, genuine tested and approved Disposable Syringes 11
consumables will provide you with solid, reliable support, giving
Solid Phase Extraction
you all you need to succeed. But our solutions don’t stop there. (SPE) 12-15

SPP Column 16-18

At PerkinElmer, we're committed to providing you with the best
UHPLC Column 19
selection of solutions for all your Chromatography needs,
Conventional Column 20-27
regardless of make, model or manufacturer. We have invested
heavily in growing our portfolio, at your request, to bring you Cartridge Column 28-33

the best performing and productivity enhancing consumables Sample Loops and
and accessories for all your gas chromatograph systems. Our Tubing 34

consumables can be depended upon to meet the most demand- Fingertight Fittings 35
ing performance and throughput needs for today's analytical, Vacuum Degassing Kits 36
process monitoring and quality control facilities.
Solvent Filtration and
Mixers 37

Injection Valves and

Rotor Seals 38

Flow Cells and Pump

Accessory kits 39

Detector Lamps 40

Generators 41

www.perkinelmer.com/supplies 1
 FLEXAR SQ 300 MS

VERIFY.
QUANTIFY.
SIMPLIFY.
The Flexar SQ 300 MS
Ideal for a variety of analyses, this single quadrupole detector
brings the unsurpassed sample insight of mass spectrometry to
liquid chromatography applications. The Flexar SQ 300 MS™ is an
integral part of the powerful and exible Flexar LC product line. It
features a revolutionary interchangeable probe and unique multi-
stage ion path for exceptional sensitivity.
Measuring has never been easier with the Flexar SQ 300 MS. It
provides the simplest route to exactly the information you need
— quickly. As part of the Flexar LC platform, labs can expect
exceptional chromatography coupled with an innovative rugged
Spare Probe Assemblies / Dielectric Capillary
single quadrapole mass spectrometer that offers:
Description Part No.
• Exceptional front-end chromatographic separation capabilities ESI Probe and Capillary Entrance Cone Kit MZ300503
• Flexible, modular components to suit any LC/MS analytical need Capillary Entrance Cone MZ105094
• idest selection of operating pressures to address ESI Probe MZ109197
throughput needs Dielectric Capillary MZ105057-
60180
The Flexar SQ 300 MS has three ion source options that allow you Quartz Tube for APCI MZ109004
to speci cally address your analytical needs: Corona Needle for APCI MZ301193

Calibration solutions are required for instrument installation and for routine operations.
• Ultraspray™ Electrospray Interface (ESI)
• Dual-probe Ultraspray2™ ESI Calibration Solutions
• Atmospheric Pressure Chemical ionization (APCI)
Description Part No.
Calibration Mix LC/MS (+) ION Mix MZ301198
Powered by the PerkinElmer Chromera® software, instrument
Calibration Mix LC/MS (-) ION Mix MZ301199
control and data acquisition are faster and easier than ever.

Flexar SQ 300 MS Test Kits

Description Part No. Description Part No.

Flexar SQ 300 MS N2910801 SQ300 Installation Performance Test Kit MZ300061
Flexar SQ 300 MS with Diverter Valve N2910800 SQ300 Customer Performance Test Kit MZ300085
SQ300 ESI Positive and Negative Test Kit MZ300096

Spare Syringes and Needles (for Syringe Pump)

Description Part No.
Hamilton 500uL Gas Tight Syringe 09220120
(No Needle)
Hamilton Replacement 6-pack of Needles 09220122

2 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 FLEXAR SQ 300 MS ACCESSORIES

Custom Benches
Description Part No.
120V Bench with Ventilated, Sound N2911197
Dampened Pump Enclosure
120V Bench with Monitor/Keyboard Arm, Side Shelf N2911196
and Ventilated, Sound Dampened Pump Enclosure
230V Bench with Ventilated, Sound Dampened N2911199
Pump Enclosure
230V Bench with Monitor/Keyboard Arm, Side Shelf N2911198
and Ventilated, Sound Dampened Pump Enclosure
Roughing Pump Box with Acoustically Insulated N8141230
Pump Enclosure
Uninterrupted Power Supply (UPS) N0777511

der Convenience Kits

Description Pkg Part No.
Sound Reducing Pump Box N9300707
Convenience Kit
This pump is made from noise adsorbing material, which is oil and
9 mm 2 mL Screw Top Clear Vial 100
water resistant.
9 mm Blue Screw Top Cap with Clear 100
Non-slit PTFE/Silicone (LC Clean)
• Average 12 dB sound level decrease at 1.5 meters (by comparison:
e-slit Cap/Clear N9300708
decreasing sound by 10 dB results in a 50% sound reduction). Vial Convenience Kit
• Heat exchange is controlled by two internal silent fans guaranteeing 9 mm 2 mL Screw Top Clear Vial with 9 100
ient air circulation (about 200 m3/h). mm Gray Screw Top Cap
• No tools needed to access the pump compartment making routine 9 mm Gray Screw Top Cap with Clear 100
maintenance and oil change easy to be performed. Pre-slit PTFE/Silicone (LC Clean)
N9300719
Vial Convenience Kit

Description Part No. 9 mm 2 mL Screw Top Amber Vial 100

Sound Reducing Pump Box MZ301123 9 mm Blue Screw Top Cap with Clear 100
Non-slit PTFE/Silicone (LC Clean)

LCMS Filtration System Vial Convenience Kit

e-Slit Cap/Amber N9300720

High Capacity Hydrocarbon Trap 9 mm 2 mL Screw Top Amber Vial 100

• Contains 750 cc of preconditioned 9 mm Gray Screw Top Cap with Clear 100
activated charcoal. Pre-slit PTFE/Silicone (LC Clean)

• Stainless steel body. ¼” brass compression

ttngs with ferrules for installation. LCMS Caps
• Maximum pressure 200 psi.
Description Pkg Part No.
• Recommended w rate up to 2 L per minute.
• Will remove hydrocarbon impurities (50 ppm or less) 9 mm Screw Cap Blue PTFE (Natural)/ 100 N9306362
Silicone (White) Ultra-low Bleed, Non-slit
from inert gases, nitrogen and hydrogen at room temperature
9 mm Screw Cap Blue PTFE (Natural)/ 1,000 N9306363
to low ppb range. Silicone (White) Ultra-low Bleed, Non-slit
• Capacity of 67 g of hydrocarbons C5 and heavier.
9 mm Screw Cap Blue PTFE (Natural)/ 100 N9306364
• 10 µ stainless steel porous frits protect gas stream Silicone (white) Ultra-low Bleed, Slit
from particulates. 9 mm Screw Cap Blue PTFE (Natural)/ 1,000 N9306365
• Individually helium leak tested. Shipped lled wth helium. Silicone (white) Ultra-low Bleed, Slit

Description Part No.

LCMS Filtration System N9301208

www.perkinelmer.com/supplies 3
 FLEXAR UHPLC

POWER AND
NEW
FLEXIBILITY
IN UHPLC FX-15
ULTRA HIGH
PERFORMANCE LC
18,000 PSI

FX-10
ULTRA HIGH
PERFORMANCE LC
10,000 PSI

LC
CONVENTIONAL LC
6,000 PSI
PERFORMANCE

LC
CONVENTIONAL LC
MANUAL INJECTION

NEW FLEXAR™ UHPLC SYSTEM FROM PERKINELMER

With Flexar UHPLC, you’ll be able to transfer your existing
methods to achieve faster, more predictable results while reducing
your solvent consumption by up to 90%. The FX-15’s patented,
dual reciprocating, 18,000 psi pump offers the widest operating PRODUCTIVITY

range with exceptional compositional accuracy and retention time

Flexar UHPLC Systems
repeatability. The value and performance of the FX-10 microbinary
pump offers minimal disruption in your method development Description Part No.
phase and analytical approach so you can easily port your existing Flexar FX-10 UHPLC Platform NFLR0210
methods. And with the powerfully easy new Chromera® software, Flexar FX-10 UHPLC Pump N2910640
instrument control is taken to a whole new level. Performance, Flexar FX-10 UHP Pump Master N2910641
edictability — Flexar.
“Flexar Bottle Manager, 3-channel Vacuum N2600581
Degassing” Tubing Kit

ar UHPLC
Flexar UHPLC Systems
• Improved separa iency
• Up to 90% reduction in solvent cons ving you money Description Part No.
Flexar FX-15 UHPLC Platform NFLR0215
• Up to 10x productivity g g you do more in less time
Flexar FX-15 UHPLC Pump N2910531
“Flexar Bottle Manager, 3-channel Vacuum N2600581
Degassing” Tubing Kit

4 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 FLEXAR ACCESSORIES

High Pressure Analytical Static Mixers FX-15 UHPLC Pump Accessories

Static Mixers are important in facilitating complete mobile phase Description Part No.
blending, resulting in improved retention performance. These Replacement 0.5 µm Filter Frits for FX-15 (4-pack) N2911224
mixers should be used as part of FX10, or FX15 LC systems, where High Pressure FX-15 Piston Seal Kit N2911221
high pressure blending is required. Mixers incorporate a highly (Need 4 per FX-15)

ef cient cross- w shearing mechanism which produces vortex Piston Wash Bottle Kit N2601616
sheer mixing over a wide range of volumes. They are now offered 2,500 µL Sample Syringe Starting Kit N2930313
in 18,000 psi pressure max avor.
A good rst choice selection is a volume of about half the w rate
FX-10 and FX-15 UHPLC System Accessories
volume. For example, when pumping at a 0.5 mL/min w rate, a Description Color Size Part No.
350 μL mixer provides good mixing with very good baseline stability. ID PEEK Tubing
0.004" Black 50 cm N2916200
Description Part No.
0.005" Red 50 cm N2916056
Assembly, SS N2911200
0.004" Black 76 cm N2916260
Assembly, SS N2911201
0.005" Red 66 cm N2916262
Assembly, SS N2911202
Connector Tubing, Stainless Steel
Assembly, SS N2911205
Flexar ISO/Binary/Quaternary Pump to Autosampler N2916210
Assembly, SS N2911203
Dual Mixer Connection (≤ 250 µL) N2916216
150 L High Pressure Binary High Pressure Mixing-T, SS N2911206
Dual Mixer Connection (³350 µL) N2916217
, SS N2911170
FX-10 Pump A (Upper) to T-mixer N2916213
Binary High Pressure T-Mixer, SS, N2911212
FX-10 Pump B (Lower) to T-mixer N2916214
Binary High Pressure T-Mixer, SS, N2911210
FX-10 Purge Valve to Filter/Scavenger N2916212
Binary High Pressure T-Mixer, SS, N2911211
FX-10 Injector to Column N2916215
T-connector, SS N2911127
FX-10 Pump A (Upper) to Dual Mixers N2916218
FX-10 Pump B (Lower) to Dual Mixers N2916219
Syringes and Kits FX-15 Pump to In-line Mixer N2916222
PerkinElmer syringe kits for high pressure applications are FX-15 Pump to Dual Mixers N2916224
manufactured for precise liquid delivery. All of these glass syringes FX-15 Injector to Column, Standard N2916223
come with precision stainless steel plungers. They are used for FX-15 Injector to Column, 33 cm N2916201
sampling and shing and are available in a large variety of sizes. 3 cm Scavenger Cartridge Holder 02580178
Syringes Spheri5 Scavenger Columns (5-pack) 02580202
30 mL Priming Syringe 09904849
Description Part No.
FX-15 C18 Scavenger Column Kit N2910765
Sample Needle w/Fittings N2936009
FX-15 C18 Scavenger Columns (2/pkg) 02580223
Air Guide Needle (62 mm) N2936000
Bio Compatible Sample Needle w/Tubing Connector N2936010
Needle (80 mm) N2936342 Microsyringes (For Manual Injection)
Syringes are used for accurate and precise liquid delivery. Each
Syringe Kits
syringe is hand- tted to assure maximum accuracy. Our syringes
Dimension Buffer Tubing Part No. are composed of glass barrels and precision stainless steel
100 µL 200 µL N2936051 needles. The needle features a blunt tip, required for use with a
250 µL 500 µL N2936052 Rheodyne injector.
500 µL 1,000 µL N2936053
Description Part No.
1,000 µL 2,000 µL N2936054
10 µL Syringe 09904937
2,500 µL 2,000 µL N2936055
25 µL Syringe 09904823
50 µL Syringe 09904941
100 µL Syringe 09904822
Rheodyne 22-gauge Blunt Needle with Luer Hub 09904943

www.perkinelmer.com/supplies 5
 FLEXAR AUTOSAMPLERS

INJECT WITH
GREATER PRECISION
Flexar Autosamplers
Best-in-class cycle time. Wide injection volume range. Excellent
injection reproducibility. Precisely what you need to increase your
sample throughput and ef ciency. Flexar or Series 225/275
Autosampler Trays and Accessories
Features and Be Description Part No.
• Injects sample volumes as l t pressures up to 18,000 psi 25-Position 6 mL Vial Sample Tray N2936045
with the FX UHPLC Autosampler
80-Position 2 mL Vial plus (5) 6 mL Vial Tray N2936046
• Loads sample in only 8 seconds (in parti ll mode) (for derivatization)

• ee injection modes: full loop, parti ll and µL-pickup with no 80-Position 2 mL Vial Tray with Dilution Tray N2930676
sample waste 96-well Microtiter Adapter N9302562
• Peltier cooling/heating mode option for operation at 4 °C to 40 °C. 96-well ‘Deep-well’ Microtiter Adapter N9302560
Will reach 4 °C ± 2 °C, achievable even at ambient temperatures up 96-well 7 mm Pre-slit Silicone Plate Mat/Seal N9302555
to 25 °C.
96-well ‘Deep’ Microtiter Plate Adaptor N2936048
(Supports Dual Microtiter Plates)
All Flexar autosamplers can be upgraded in the eld for Peltier 96-well ‘Shallow’ Microtiter Plate Adaptor N2936049
cooling and heating. All Flexar autosamplers include: (Supports Dual Microtiter Plates)
• 100‐sample tray • Service Manager SW CD 384-well Microtiter Adapter N2936050
• 100 2 mL vials • User’s Manual 100-Position 2 mL Vial Sample Tray N2936042
• 100 2 mL screw top cap with • Nuts and ferrules (200) 0.2 mL Microvial plus (5) 2 mL Vial Tray N2936043
cross slit vial septum • All fuses for 120–240 V 250 µL Syringe Tip Replacements (10/pkg) N2936003
• Tubing to connect to • All cables required for connection Flexar/225 Biocompatible Upgrade Kit (w/valve) N2930675
waste reservoir to a TotalChrom or Chromera
⁄16" OD x 0.038" ID Te on Tubing (AS Transfer Line)
1
02506495
• I/O cable (Pump Start/Ready‐In) Operating Environment

Flexar Autosamplers and Upgrade Kits

Description Part No.
Flexar LC Autosampler
High throughput autosampler with exceptionally low carryover. Operates up to 6,100 psi (420 bar). full-loop, N2930660
aste). Peltier cooling and heating options av ades. Comes standard with 100 µL loop and
.
Flexar Peltier LC Autosampler
High throughput autosampler with exceptionally low carryover. Operates up to 6,100 psi (420 bar). full-loop, N2930661
aste). Built-in Peltier cooling comes standard. Peltier heating option av ade. Comes
.
Flexar FX UHPLC Autosampler
High throughput UHPLC autosampler with exceptionally low carryover. Operates up to 18,000 psi (1,241 bar). N2930664
full-loop, aste). Built-in Peltier cooling comes standard. Peltier heating option av ade.
.
Field Upgrade Peltier Kits
Flexar and Series 225 Upgrade Kit (from standard to Peltier Cooling option) N2930672
Flexar and Series 225 Upgrade Kit (from standard to Peltier Cooling and Heating option) N2930673
Flexar and Series 225 Upgrade Kit (from Peltier Cooling to Peltier Cooling and Heating option) N2930669

6 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 CHROMATOGRAPHY DATA SYSTEM

CHROMERA® CHROMATOGRAPHY
DATA SYSTEM NEW
THE POWERFULLY EASY CDS
One click and you’ll see: CDS doesn’t get any easier than Chromera. Combining intuitive
, Chromera delivers the most
streamlined way to get from the analysis you need to the information your enterprise
demands. It’s powerfully easy.

Information-rich Desktop offers instrument control at-a-glance

Featur

Powerful Easy
• Instrument Control: NEW patented “Device Descriptors” control • Shallow footprint GUI: Most functions only one or two clicks away,
instruments. No channels limitation, trackable instrument status using familiar Micr ™ Outlook user interface
• Designed for UHPLC: interactive graphical methods optimization • Simple and obvious method editor: a single-point access to
environment with immediate feedback; ability to overlay pressure all instrument parameters, regardless of how complex the
pr le r er run. Extend runtime of chromatogram in real time instrument c uration; Convenient tools for point-and-click
method optimization
• Graphics: Multichannel graphics for multiple signal channel
monitoring (including system status channels to get ‘the pulse • Intuitive Sequence Wizard: Create complex sample tables easily;
of the system’ real-time) Convenient auto ll and other editing functions; Decision Points
for real-time and post-processing data evaluation
• Feedback: Instrument-at-a-glance status panel provides real-time
feedback of complete LC system • Peak Integration Wizard: Makes integration process easy and straight
forward
• Batch Sequencing: all the f g up and reviewing a
sequence and results are within a single environment • Report Wizard: allows creating numerous styles of reports and
printouts using the same tool

www.perkinelmer.com/supplies 7
 FLEXAR AUTOSAMPLER ACCESSORIES

LC VIALS, CAPS
AND SEPTA KITS
PerkinElmer Supplies – Tested and Approved 2.0 mL Caps and Septa*
We have expanded our product selection to include additional vial Description Pkg. Part No.
types, new version caps and mating septa in a kit format for easier 11 mm Snap Cap Polyethylene 1,000 04978532
customer ordering. The following pages contain both current Cross-cut Slit (no Septum)
and new product information. Tested to meet your application 11 mm Snap Cap Polyethylene 1,000 N9307023
requirements and guaranteed for t and compatibility. Single Slit (no Septum)
8 mm Black Screw Caps (without septa) 100 N9303441

LC Vials, Caps and Septa Kits 8 mm SEPTA-PTFE Red Rubber No CAP

(for Cap N9303441)
100 N9303442

PerkinElmer offers a variety of vials, caps and septa to ful ll 8 mm Screw Cap PTFE/Silicone Septa 100 N9303449
your application needs. Our screw thread vial caps use the
9 mm PTFE/Silicone Screw Cap Blue 100 N9306202
revolutionary Inter-Seal®. Using a process that bonds silicone/
10 mm PTFE/Red Rubber Screw Cap Black 100 N9306206
PTFE and other elastomeric compounds directly into thermoplastic
10 mm PTFE/Silicone Single Slit 100 N9306052
closures eliminates liner fallout, while still providing the excellent Screw Cap Black
resealability and multiple injection capability. No adhesives are
10 mm PTFE/Silicone No Slit Screw Cap Black 100 N9306205
used in this process, bonding the cap and septa at the molecular
11 mm Crimp Green Aluminum Cap 100 N9302684
level of plastic and rubber. These septa have a very broad w/T ®
Rubber Septa
chemical resistance and can be used in many markets including: 11 mm Crimp Red Aluminum Cap 100 N9302685
environmental, diagnostic packaging, pharmaceutical packaging, w/T ®
Rubber Septa
cosmetic and food packaging. 11 mm Crimp Blue Aluminum Cap 100 N9302686
w/T ®
Rubber Septa

2.0 mL Glass Vials* 11 mm Crimp Silver Aluminum Cap 100 N9306015

w/T ®
Rubber Septa
Description Pkg. Part No. 11 mm Crimp Silver Red Rubber 100 N9306230
2 mL 11 mm Clear Glass Crimp Vials 100 N9301385 11 mm Crimp Silver PTFE/Silicone 100 N9306228
2 mL 11 mm Clear Crimp Wide Opening 100 N9306223 11 mm Crimp Silver PTFE/Silicone/PTFE 100 N9306229
Vials with Write-On Patch and Fill Lines
* Also see Convenience Kits for additional vials and caps, sold separately.
2 mL 11 mm Amber Glass Crimp Vials 100 N9302680
2 mL 11 mm Amber Crimp Wide Opening 100 N9302679
Vials with Write-On Patch and Fill Lines 0.2 mL Micro Vials, Caps and Septa
2 mL 11 mm Clear Snap Top Vial 100 N9303418 Description Pkg. Part No.
2 mL 11 mm Clear Snap Wide Opening 100 N9306207 Caps/Septa
Vials with Write-On Patch and Fill Lines
8 mm Silver Aluminum Crimp Cap 1,000 03300806
2 mL 11 mm Amber Snap Wide Opening 100 N9306208 PTFE/Rubber
Vials with Write-On Patch and Fill Lines
PTFE/Pre-Slit White Silicone Polyethylene 1,000 N9302141
2 mL 8 mm Screw Top Clear Glass Vials 100 N9302945 Press-On Cross-Slit Cap for 8 mm Vials
2 mL 9 mm Clear Screw Top Vials 100 N9306201 Crimp Top Vials
2 mL 9 mm Screw Top Amber Vials 100 N9306220 Glass Microvials, Tapered, 0.2 mL, 500 N9302136
2 mL 9 mm Clear Screw Top Vial 100 N9307801 8 mm o.d. Top**
with Write-On Patch and Fill Lines Autosampler Vial Sleeve
2 mL 9 mm Screw Top Amber Vials 100 N9307802 12 x 32 Glass Vial Support Sleeve 25 N9307027
with Write-On Patch and Fill Lines for 2 mL 8 mm Micro Vials (N9302136)
2 mL 10 mm Clear Glass Screw Top Vials 100 N9306053 Micro Vial Kit
with Write-On Label and Fill Lines
Polyethylene Includes 300 μL Tapered Vial, 500 N9306080
2 mL 10 mm Amber Glass Screw Top Vials 100 N9306057 Blue Polypropylene Cap with Bonded
with Write-On Label and Fill Lines Silicone/PTFE Septa

** To be used with Glass Vial Support Sleeve (N9307027).

8 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 VIALS, CAPS, AND KITS

UHPLC – General Starter Kit Contents Qty/Pack Part No.

Description Part No. 50 cm x 0.007" ID ⁄16" SS Tubing

1
2 00891480

UHPLC – General Starter Kit N2930801 5' x 0.007" ID 1⁄16" PEEK Tubing, Yellowish- 1 N9302650
Tan
Contents Qty/Pack Part No. 5' x 0.005" ID 1⁄16" PEEK Tubing, Red 1 N2916262
Open-End Wrench 2 09907233 30 mL Priming Syringe 1 09904849
Adjustable Wrench 1 N9301326 2 mL clear write-on vials w/10 mm Pre-Slit 1 N9300695
Reversible Screw Driver 1 N9301480 PTFE/Silicone Caps; 100/pk

Parker 1 09903289 Cable/Tubing Organizer Clip 3 NX598006

Parker 10 09903980
1
⁄16" Parker errules 10 00873032
1
⁄16" Valco SS Ferrules 6 09903891
Finger-Tight Fittings, PEEK, 5.5K psi Max. 1 N9307822 Autosampler Vial Glass Inserts
(5/pk)
Optimize Technologies EZP (Hand-Tight 1 N9306301 Description Pkg. Part No.
Fitting to 13K psi, Wrench-tight to 20K psi) Vial Glass Inserts:
1
⁄16" Tefzel Nuts 2 09920381 6 mm x 29 mm Clear Glass Insert, Rimless Pulled 100 N9300703
1
⁄16" Tefzel Reverse Ferrules 2 09920382 Point with Bottom Spring, 250 μL Usable Volume
1
⁄8" Tefzel Nuts 4 N2601189 6 mm x 31 mm Clear Glass Flat Bottom Insert, 100 N9300704
400 μL Usable Volume
1
⁄8" Tefzel Reverse Ferrules 4 09903771
Use with: N9306057
PEEK Tubing Cutter (Includes 5 Extra Blades) 1 N9307820 10 mm 2 mL Amber Screw Top Vial
50 cm x 0.005" ID 1⁄16" SS Tubing 3 02507060 w/White “P” Icon w/Patch

5' x 0.004" ID 1⁄16" PEEK Tubing, Black 1 N2916261 10 mm 2 mL Clear Screw Top Vial N9306053
w/White “P” Icon w/Patch
5' x 0.005" ID ⁄16" PEEK Tubing, Red
1
1 N2916262
11 mm Clear Large Opening Snap Ring Vial N9303418
30 mL Priming Syringe 1 09904849 w/Black “P” Icon
2 mL Clear Write-On Vials w/10 mm Pre-Slit 1 N9300695 11 mm Clear Large Opening Crimp Vial N9306231
PTFE/Silicone Caps; 100/pk w/Black “P” Icon
Cable/Tubing Organizer Clip 3 NX598006 9 mm 2 mL Amber Screw Top Vial w/Black “P” Icon N9306220
9 mm 2 mL Clear Screw Top Vial w/Black “P” Icon N9306201
HPLC – General Starter Kit
5 mm x 29 mm Clear Glass Insert, Rimles Pulled 100 N9300705
Description Part No. Point with Bottom Spring, 150 μL Usable Volume
HPLC – General Starter Kit N2930802 5 mm x 31 mm Clear Glass Flat Bottom Insert, 100 N9300706
200 μL Usable Volume
Contents Qty/Pack Part No. Use with:
Open-End Wrench 2 09907233 11 mm Standard Amber Crimp Vial w/Black “P” Icon N9302680

Adjustable Wrench 1 N9301326 11 mm Standard Clear Crimp Vial w/Black “P” Icon N9301385

Reversible Screw Driver 1 N9301480 8 mm 2 mL Clear Screw Top Vial w/Black “P” Icon N9302945

Parker 1 09903289 6 mL Vials and Caps

Parker 10 09903980 6 mL Glass Large-Volume Vials with 125 N9302134

20 mm o.d. Top
1
⁄16" Parker errule 10 00873032
Polyethylene Press-On Slit Caps/Septa or 25 N2936083
1
⁄16" Valco SS Ferrules 6 09903891 20 mm o.d. Top Vials
Rheodyne SS Nut, Short Stem 2 09904956
Rheodyne SS Nut, Long Stem 2 09904974 Micro Vial Kit and Polypropylene Vial/Cap Kits
1
⁄16" Rheodyne SS Ferrule 4 09904947 Description Pkg. Part No.
1
⁄16" Tefzel Nuts 2 09920381 Polyethlene Micro Vial Kit – Pkg/500 Includes: 500 N9306080
1
⁄16" Tefzel Reverse Ferrules 2 09920382 300 uL Tapered Screw Top Vials (Pkg/500)
Blue Polypropylene 9 mm Caps w/Bonded
1
⁄8" Tefzel Nuts 4 N2601189 Silicone/PTFE Septa (Pkg/500)
1
⁄8" Tefzel Reverse Ferrules 4 09903771 1.5 mL Polypropylene Screw Top Amber Vial 100 N9301735
Finger-Tight Fittings, PEEK, 4K psi Max. 6 09920513 With Fill Lines, T ®
/Silicone Septa and
Cap With Single Slit
PEEK Tubing Cutter (Includes 5 Extra Blades) 1 N9307820
1.5 mL Polypropylene Screw Top Clear Vial With 100 N9301736
50 cm x 0.005" ID 1⁄16" SS Tubing 1 02507060 Fill Lines,T ®
/Silicone Septa and Cap
With Single Slit

www.perkinelmer.com/supplies 9
 EASY-TO-ORDER CONVENIENCE KITS

™
Kits (Vials and Caps/Septa sold separately.)
NEW
Description Pkg. Part No.
™
Non-Slit Cap/Vial Convenience Kit (Pkg. of 100) 100 N9300707
9 mm 2 mL Screw Top Clear Vial with 9 mm Blue Screw Top Cap Clear Non-Slit PTFE/Silicone (LC Clean™)
™
Pre-Slit Cap/Vial Convenience Kit (Pkg. of 100) 100 N9300708
9 mm 2 mL Screw Top Clear Vial with 9 mm Gray Screw Top Cap Clear Pre-Slit PTFE/Silicone (LC Clean™)
NEW ™
Non-Slit Cap/Vial Convenience Kit (Pkg. of 100) 100 N9300719
9 mm 2 mL Screw Top Amber Vial with 9 mm Blue Screw Top Cap Clear Non-Slit PTFE/Silicone (LC Clean™)
NEW ™
Pre-Slit Cap/Vial Convenience Kit (Pkg. of 100) 100 N9300720
9 mm 2 mL Screw Top Amber Vial with 9 mm Gray Screw Top Cap Clear Pre-Slit PTFE/Silicone (LC Clean™)

Convenience Kits (Vials and Caps/Septa sold separately.)

Description Pkg. Part No.
9 mm Pre-Slit PTFE/Silicone Screw Cap Convenience Kit (Pkg. of 100) N9300701
9 mm Pre-Slit PTFE/Silicone Screw Cap Assembly (Pkg. of 100) 1 N9306203
9 mm 2 mL Clear Screw Vial Kit (Pkg. of 100) 1 N9306201
9 mm Red Rubber/Screw Cap Convenience Kit (Pkg. of 100) N9300699
9 mm Red Rubber/Screw Cap Assembly (Pkg. of 100) 1 N9306200
9 mm Clear 2 mL Vial Kit (Pkg. of 100) 1 N9306201
9 mm PTFE/Silicone Screw Cap Convenience Kit (Pkg. of 100) N9300700
9 mm PTFE/Silicone Screw Cap Assembly (Pkg. of 100) 1 N9306202
9 mm Clear 2 mL Vial Kit (Pkg. of 100) 1 N9306201
11 mm Pre-Slit PTFE/Silicone Snap Convenience Kit (Pkg. of 100) N9300697
11 mm Pre-Slit PTFE/Silicone Snap Cap Assembly (Pkg. of 100) 1 N9303416
11 mm 2 mL Clear Snap Vial Kit (Pkg. of 100) 1 N9303418
11 mm PTFE/Silicone/PTFE Snap Convenience Kit (Pkg. of 100) N9300698
11 mm PTFE/Silicone/PTFE Clear Snap Cap Assembly (Pkg. of 100) 1 N9303417
11 mm 2 mL Clear Snap Vial Kit (Pkg. of 100) 1 N9303418
10 mm Clear Pre-Slit PTFE/Silicone Screw Cap Convenience Kit (Pkg. of 100) N9300695
10 mm Pre-Slit PTFE/Silicone Screw Cap Assembly (Pkg. of 100) 1 N9306052
10 mm 2 mL Clear Screw Vials with Write-On Patch and Fill Lines (Pkg. of 100) 1 N9306053
10 mm Amber Pre-Slit PTFE/Silicone Screw Cap Convenience Kit (Pkg. of 100) N9300696
10 mm Pre-Slit PTFE/Silicone Screw Cap Assembly (Pkg. of 100) 1 N9306052
10 mm 2 mL Amber Screw Top Vials with Write-On Patch and Fill Lines (Pkg. of 100) 1 N9306057
11 mm PTFE/Silicone Snap Convenience Kit (Pkg. of 100) N9300702
11 mm PTFE/Silicone Snap Cap Assembly (Pkg. of 100) 1 N9303419
11 mm 2 mL Clear Snap Vial Kit (Pkg. of 100) 1 N9303418
11 mm Red Rubber Crimp Convenience Kit (Pkg. of 100) N9300502
11 mm Red Rubber/Silver Crimp Assembly (Pkg. of 100) 1 N9306230
11 mm 2 mL Clear Vial Kit (Pkg. of 100) 1 N9306231
11 mm Red Rubber Crimp Convenience Kit (Pkg. of 500) N9300503
11 mm Red Rubber/Silver Crimp Assembly (Pkg. of 100) 5 N9306230
11 mm 2 mL Clear Vial Kit (Pkg. of 100) 5 N9306231
11 mm PTFE/Silicone Crimp Convenience Kit (Pkg. of 100) N9300500
11 mm PTFE/Silicone Crimp Assembly (Pkg. of 100) 1 N9306228
11 mm 2 mL Clear Vial Kit (Pkg. of 100) 1 N9306231
11 mm PTFE/Silicone/PTFE Crimp Convenience Kit (Pkg. of 100) N9300501
11 mm PTFE/Silicone/PTFE Crimp Assembly (Pkg. of 100) 1 N9306229
11 mm 2 mL Clear Vial Kit (Pkg. of 100) 1 N9306231

10 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 SYRINGE FILTERS, DISPOSABLE SYRINGES, MICRO-VIALS, BOTTLE KITS

Syringe Filters
Size Color Pkg Part No.
Nylon
0.22 μm, 4 mm Clear 200 02542900
0.45 μm, 4 mm Clear 200 02542901
0.22 μm, 13 mm Purple 100 02542902
Vials with Fused Glass Sample Inserts 0.45 μm, 13 mm Purple 100 02542903

Description Pkg Part No. 0.22 μm, 17 mm Yellow 100 02542881

2 mL 11 mm Large Opening Crimp Top 100 N9300709 0.45 μm, 17 mm Yellow 100 02542880
0.22 μm, 25 mm Purple 100 02542904
2 mL 11 mm Large Opening Crimp Top 100 N9300710 0.45 μm, 25 mm Purple 100 02542905
Amber 0.3 mL Insert
0.22 μm, 30 mm Yellow 100 02542883
2 mL 11 mm Large Opening Snap Top Ring 100 N9300711
Clear 0.3 mL Insert 0.45 μm, 30 mm Yellow 100 02542882

2 mL 11 mm Large Opening Snap Top Ring 100 N9300712 PTFE (Hydrophobic)

Amber 0.3 mL Insert 0.22 μm, 4 mm Clear 200 02542906
2 mL 8 mm Screw Top Clear 0.1 mL Insert 100 N9300713 0.45 μm, 4 mm Clear 200 02542907
2 mL 8 mm Amber Screw Top 0.1 mL Insert 100 N9300714 0.22 μm, 13 mm Red 100 02542908
2 mL 9 mm Large Opening Screw Top 100 N9300715 0.45 μm, 13 mm Red 100 02542909
Clear 0.3 mL Insert
0.22 μm, 17 mm Red 100 02542884
2 mL 9 mm Large Opening Screw Top 100 N9300716
Amber 0.3 mL Insert 0.45 μm, 17 mm Red 100 02542910

2 mL 10 mm Large Opening Screw Top 100 N9300717 0.22 μm, 25 mm Red 100 02542911
Clear 0.3 mL Insert 0.45 μm, 25 mm Red 100 02542912
2 mL 10 mm Large Opening Screw 100 N9300718 0.22 μm, 30 mm Red 100 02542886
Amber 0.3 mL Insert
0.45 μm, 30 mm Red 100 02542885
PVDF
Liquid Chromatography Laboratory Bottle Kits
0.22 μm, 4 mm Clear 200 02542913
Description Part No.
0.45 μm, 4 mm Clear 200 02542914
1x5L Bottle with Cap and T 2m PTFE N2601610
0.22 μm, 13 mm Yellow 100 02542915
1
⁄8" Tubing and 1x10um SS Solvent Frit
0.45 μm, 13 mm Yellow 100 02542783
1x2L Bottle with Cap and T 1m PTFE N2601611
1
⁄8" Tubing and 1x10um SS Solvent Frit 0.22 μm, 17 mm Maroon 100 02542916
1x1 L Bottle with Cap and T 1m PTFE N2601612 0.45 μm, 17 mm Maroon 100 02542917
1
⁄8" Tubing and 1x10um SS Solvent Frit
0.22 μm, 25 mm Yellow 100 02542918
1x0.5L Bottle with Cap and T 1m PTFE N2601613
0.45 μm, 25 mm Yellow 100 02542919
1
⁄16" Tubing and 1x40um SS Solvent Frit
0.22 μm, 30 mm Maroon 100 02542920
1 Cap with T 1m PTFE 1⁄8" Tubing and N2601614
1x10um SS Solvent Frit 0.45 μm, 30 mm Maroon 100 02542921
1 Cap with T 1m PTFE 1⁄16" Tubing and N2601615 PTFE (Hypdrophilic)
1x40um SS Solvent Frit
0.22 μm, 4 mm Clear 200 02542922
1x0.5L Bottle with Cap and Dual T N2601616
0.45 μm, 4 mm Clear 200 02542923
2 pcs of 1m PTFE 1⁄8" Tubing (Piston Wash Function)
0.22 μm, 13 mm Red 100 02542924
1 Cap with Dual Te on Insert, 2 pcs of 1 m PTFE N2601617
1
⁄8"Tubing and 1x10um SS Solvent Frit (Piston 0.45 μm, 13 mm Red 100 02542925
Wash Function)
0.22 μm, 25 mm Red 100 02542926
0.45 μm, 25 mm Red 100 02542927
Disposable Syringes
0.22 μm, 30 mm Red 100 02542928
Description Part No. 0.45 μm, 30 mm Red 100 02542929
Disposible Syringes, Luer-Lock Tips, 1 mL (100PK) 02542890
Disposible Syringes, Luer-Lock Tips, 3 mL (200PK) 02542891
Disposible Syringes, Luer-Lock Tips, 5 mL (100PK) 02542892
Disposible Syringes, Luer-Lock Tips, 10 mL (100PK) 02542893

www.perkinelmer.com/supplies 11
 SOLID PHASE EXTRACTION (SPE)

Supra-Clean and
Supra-Poly SPE Solutions
Manufactured for guaranteed reproducibility and superior
performance, this innovative line of SPE solutions includes the
Supra-Clean® silica based and Supra-Poly® polymer based line of SPE Manifolds, Selection Kits and Application Packs
spherical media SPE cartridges and columns.
For greater method development simplicity and sample
Both utilize Precise Bed Technology® allowing columns to be throughput, PerkinElmer offers a variety of pre-prepared column
selection kits as well as application kits for you to choose from.

bed volume precision, ensuring you experience repeatability and SPE Manifolds:
optimized recovery reproducibility. • Allow you to process up to 24 samples simultaneously
Ideal for a broad array of analytes and matrices, SPE solutions are • Seamlessly integrate with columns to extend the system’s
available in a variety of formats including Large Reservoir Capacity performance and reproducibility
(LRC) columns, Polypropylene (PP) columns and cartridges, and • Feature polypropylene or stainless steel needle valves and tips
glass columns. Each technology is offered with a wide selection of with minimum dead volume
polymer and silica sorbents, and large and small sample volumes • Rugged stopcocks are reusable and provide independent
(50μL-1L) allow you to perform scalable analyses depending upon w control
your required detection limits.
oduct is delivered with an individual quality

Performance Comparison of PerkinElmer Spherical

SPE Media vs. Leading Irregular SPE Silica.
250 x 4.6 mm column
Hexane - IsoPropanol
(97/3)
24 Flow rate: 1.0 ml/min
PSI: 1 bars
UV: 254 nm
A- Tri-Terbutylbenzene
18 B- Diethyl Phthalate

12 V1

6
V2
IR60-40/63SI
Competitor SPE Supra-Clean
SPE Column SPE Column 60-50SI
0 V1 < V2
2 4 6
Spherical media and consistent particle distribution enable smaller elution
volumes and better, more reproducible extraction, purification, concentration PerkinElmer’s smaller, more homogeneous, spherical media deliver
and recovery. sharper, narrower peaks for faster, more accurate sample analysis.

12 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 SOLID PHASE EXTRACTION (SPE)

Manifolds, Selection Kits and SPE Manifolds and Accessories

Application Packs to Streamline Description Pkg Part No.

Your Sample Preparation Cover, Gasket, 12 Stopcocks 12 N9306621

Cover, Gasket, 24 Stopcocks 24 N9306628
For greater method development simplicity and sample
Drying Attachment 12 Positions 12 N9306625
throughput, PerkinElmer offers a variety of pre-prepared kits and
Drying Attachment 24 Positions 24 N9306632
column manifolds.
Glass Chamber 12 Positions 12 N9306620

Features and Be Glass Chamber 24 Positions 24 N9306627

Needles Polypropylene 12 Positions 12 N9306622
• Allow you to process up to 24 samples simultaneously
Needles Polypropylene 24 Positions 24 N9306629
• Seamlessly integrate with columns to extend the system’s
performance and reproducibility Needles Stainless Steel 12 Positions 12 N9306623
Needles Stainless Steel 24 Positions 24 N9306630
• Feature polypropylene or stainless steel needle valves and tips with
minimum dead volume Stopcocks 12 Positions 12 N9306624

• Rugged stopcocks are reusable and provide independen w control Stopcocks 24 Positions 24 N9306631
Vacuum Manifold Complete Set 12 Positions 12 N9306619
Vacuum Manifold Complete Set 24 Positions 24 N9306626
SPE Selection Kits
Selection kits enable quick column selection for development of reproducible and repeatable SPE methods.

Description Weight/Volume Pkg Part No.

Extraction of Acidic, Basic and Neutral Compounds from Aqueous or Organic Matrix 100mg/3mL 50 N9306602
Pre-Concentration of Hydrophobic Compounds from Aqueous Matrix 200mg/3mL 50 N9306595
Pre-Concentration of Hydrophobic Compounds from Aqueous Matrix 200mg/6mL 50 N9306594
Extraction of Hydrophobic Compounds from Aqueous Matrix 500mg/3mL 50 N9306597
Pre-Concentration of Hydrophilic Compounds 500mg/3mL 50 N9306599
Removal of Polar Compounds from Aqueous and Organic Matrix 500mg/3mL 50 N9306601
Extraction of Carboxylic Acids and Strong Bases from Aqueous Matrix 500mg/6mL 30 N9306603
Extraction of Hydrophobic Compounds from Aqueous Matrix 500mg/6mL 30 N9306596
Extraction of Weak Bases from Aqueous Matrix 500mg/6mL 30 N9306604
Pre-Concentration of Hydrophilic Compounds 500mg/6mL 30 N9306598
Removal of Polar Compounds from Aqueous and Organic Matrix 500mg/6mL 30 N9306600

SPE Application Packs

Ideal for extraction of known entities from a variety of matrices, our packs are expertly tailored to meet your application needs and are
designed to support major EPA methods and applications.

Description Weight/Volume Pkg Part No.

Extraction of PAH from Soil and Oil 1.5g/6mL 30 N9306609
Extraction of PAH from Soil and Oil (Glass Straight Column) 1.5g/6mL 30 N9306610
Extraction of PAH from Water Containing Humic Acids 1.5g/6mL 30 N9306608
Extraction of PCB from Oil 1g/3mL 50 N9306616
Extraction of Bisphenol A from Aqueous Matrix 1g/6mL 30 N9306613
Extraction of Oil and Grease from Aqueous Matrix-EPA 1664 1g/6mL 30 N9306611
Extraction of PCB from Oil 1g/6mL 30 N9306617
Extraction of SVOC from Water-EPA 525 1g/6mL 30 N9306618
Extraction of Basic Drugs from Biological Fluids 200mg/3mL 50 N9306605
Extraction of PAH from Water or Soil 4g/6mL 30 N9306606
Extraction of PAH from Water or Soil (Glass Straight Column) 4g/6mL 30 N9306607
Extraction of Oil and Grease from Aqueous Matrix-EPA 1664 500mg/3mL 50 N9306612
Extraction of Pesticides and Herbicides from Aqueous Matrix 500mg/6mL 30 N9306614
Extraction of Steroids from Biological Fluids 500mg/6mL 30 N9306615

www.perkinelmer.com/supplies 13
 SOLID PHASE EXTRACTION (SPE)

Complete Consistency. . Weight/Volume Pkg Part No.

SPE Supra-Clean C18 Column
Weight/Volume Pkg Part No.
100mg/1mL 100 N9306478
SPE Supra-Clean Cyano (CN-S) Column
100mg/3mL 50 N9306523
500mg/3mL 50 N9306645
1g/6mL 30 N9306422
500mg/6mL 30 N9306644
200mg/3mL 50 N9306462
SPE Supra-Clean Florisil (FL-S) Column
2g/15mL 20 N9306479
1g/6mL 30 N9306413
2g/25mL 20 N9306475
200mg/3mL 50 N9306511
2g/6mL 30 N9306430
2g/15mL 20 N9306514
500mg/3mL 50 N9306438
2g/25mL 20 N9306515
500mg/6mL 30 N9306448
2g/6mL 20 N9306513
50mg/1mL 50 N9306476
500mg/3mL 50 N9306512
200mg/6mL 30 N9306634
500mg/6mL 30 N9306494
500mg/6mL 30 N9306640*
SPE Supra-Clean Florisil (FL-S) Pesticide Grade Column
500mg/3mL 50 N9306642*
1g/6mL 30 N9306436
SPE Supra-Clean LCC Column
200mg/3mL 50 N9306516
500mg/3mL 50 N9306643
2g/15mL 20 N9306443
500mg/6mL 30 N9306641
2g/25mL 20 N9306447
SPE Supra-Clean Phenyl (PH-S) Column
2g/6mL 30 N9306470
100mg/1mL 100 N9306524
500mg/3mL 50 N9306400
100mg/3mL 50 N9306525
500mg/6mL 30 N9306517
1g/6mL 30 N9306527
SPE Supra-Clean Amino (NH2-S) Column
200mg/3mL 50 N9306490
100mg/1mL 100 N9306410
500mg/3mL 50 N9306421
100mg/3mL 50 N9306529
500mg/6mL 30 N9306526
200mg/3mL 50 N9306530
50mg/1mL 50 N9306401
500mg/3mL 50 N9306414
SPE Supra-Clean REC18 Column (full accessible surface)
500mg/6mL 30 N9306531
100mg/1mL 100 N9306520
50mg/1mL 50 N9306528
100mg/3mL 50 N9306455
SPE Supra-Clean Polyamine (P6) Column
1g/6mL 30 N9306491
500mg/3mL 50 N9306518
200mg/3mL 50 N9306521
500mg/6mL 30 N9306434
500mg/3mL 50 N9306522
SPE Supra-Clean Silica (SI-S) Column
500mg/6mL 30 N9306457
100mg/3mL 50 N9306532
50mg/1mL 50 N9306519
1g/6mL 30 N9306404
200mg/1mL 30 N9306633
200mg/3mL 50 N9306444
SPE Supra-Clean Strong Anion Exchange (SAX) Column
2g/15mL 20 N9306534
100mg/1mL 100 N9306471
2g/25mL 20 N9306535
100mg/3mL 50 N9306554
2g/6mL 20 N9306533
200mg/3mL 50 N9306482
500mg/3mL 50 N9306402
500mg/3mL 50 N9306555
500mg/6mL 30 N9306466
500mg/6mL 30 N9306556
SPE Supra-Clean 300Å C4 Column
50mg/1mL 50 N9306553
100mg/1mL 100 N9306591
* No end-capping
100mg/3mL 50 N9306592
200mg/3mL 50 N9306593
50mg/1mL 50 N9306590

14 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 SOLID PHASE EXTRACTION (SPE)

Weight/Volume Pkg Part No. Weight/Volume Pkg Part No.

SPE Supra-Clean Strong Cation Exchange (SCX) Column SPE Supra-Poly Extreme Capacity 1500 m2/g (XC) Column
100mg/1mL 100 N9306432 100mg/1mL 50 N9306403
100mg/3mL 50 N9306537 100mg/3mL 50 N9306440
200mg/3mL 50 N9306538 1g/15mL 20 N9306503
500mg/3mL 50 N9306539 200mg/3mL 50 N9306428
500mg/6mL 30 N9306540 30mg/1mL 50 N9306441
50mg/1mL 50 N9306536 500mg/6mL 30 N9306405
SPE Supra-Clean Weak Cation Exchange (WCX) Column 50mg/1mL 50 N9306500
100mg/1mL 100 N9306545 60mg/1mL 50 N9306501
100mg/3mL 50 N9306546 60mg/3mL 50 N9306502
200mg/3mL 50 N9306547 200mg/6mL 30 N9306635
500mg/3mL 50 N9306420 SPE Supra-Poly Extra Wide Particle 1200 m2/g (XWP) Column
500mg/6mL 30 N9306407 100mg/1mL 50 N9306506
50mg/1mL 50 N9306544 100mg/3mL 50 N9306508
SPE Supra-Clean Mixed-Mode (MM1, MM2, MM3) Column 1g/15mL 20 N9306510
100mg/1mL 100 N9306542 200mg/3mL 50 N9306509
(MM1)
30mg/1mL 50 N9306504
100mg/3mL 50 N9306419
500mg/6mL 30 N9306418
(MM1)
50mg/1mL 50 N9306427
200mg/3mL 50 N9306543
(MM1) 60mg/1mL 50 N9306505
500mg/3mL 50 N9306481 60mg/3mL 50 N9306507
(MM1)
SPE Supra-Poly ATH, ATL, AEV Polymer Column
500mg/6mL 30 N9306416
200mg/6mL 30 N9306636
(MM1)
(ATH)
50mg/1mL 50 N9306541
200mg/3mL 50 N9306638
(MM1)
(ATH)
100mg/1mL 100 N9306549
100mg/3mL 50 N9306646
(MM2)
(ATH)
100mg/3mL 50 N9306550
200mg/6mL 30 N9306637
(MM2)
(ATL)
200mg/3mL 50 N9306551
200mg/3mL 50 N9306639
(MM2)
(ATL)
500mg/3mL 50 N9306411
100mg/3mL 50 N9306647
(MM2)
(ATL)
500mg/6mL 30 N9306552
100mg/3mL 50 N9306648
(MM2)
(AEV)
50mg/1mL 50 N9306548
SPE Supra-Clean Silica (SI-S) Cartridge (full accessible surface)
(MM2)
1300mg 50 N9306586
500mg/6mL 30 N9306649
(MM3) 300mg 50 N9306584
700mg 50 N9306585
SPE Supra-Clean C18 Cartridge (full accessible surface)
1690mg 50 N9306589
390mg 50 N9306587
910mg 50 N9306588

www.perkinelmer.com/supplies 15
 BROWNLEE SPP COLUMNS

INNOVATION
SPP Particle Technology

TO THE CORE
1.7 µm 2.7 µm

Brownlee SPP HPLC and

UHPLC Column Solutions
0.5 µm
Say goodbye to the limitations of traditional columns and
experience greater speed, lasting durability and better results from
your Liquid Chromatography instrument. Smaller in size and innovative in design, superficially porous particles are made
by fusing a porous silica layer to a solid inner core.
Brownlee Super cially Porous Particle (SPP) columns produce
sharper peaks and faster separation results. These results are
possible due to their breakthrough particle design and size.
Brownlee SPP columns use 2.7 µm particles comprised of a thin
outer shell of high-quality porous silica fused to a solid inner
core. This advanced design allows for a shorter diffusion path,
reducing the time solute molecules spend inside the particles
while passing through the stationary phase.

Brownlee SPP Phases and Applications

Coverage Temp Limit
UHPLC Phases* PoreSize (Å) (µmol/m^2) pH Range (°C) Applications Chromatographic Properties
C18 90 3.5 2 to 9 60 General Purpose Octadecyl phase for reversed A high-purity column that exhibits excellent
phase separations. peak shape for a wide range of compounds.
C8 90 3.7 2 to 9 60 General Purpose Octyl phase for reversed High purity reversed phase packing that
phase separations when less retention than exhibits excellent peak shape for a wide
a C18 is desired. range of compounds.
HILIC** 90 N/A 2 to 8 60 General purpose bare silica column for normal High purity silica substrate.
phase and HILIC applications.
Peptide ES-C18** 160 2.0 1 to 8 90 Sterically protected ligand (isobutyl - side chains), The 160 Angstrom pore size was specially
results in an extra stable bonded phase at low pH chosen for the molecular weight range of
where most peptide separations are performed. peptides. The ligand was chosen due to its
sterically protected bonding technology that
inhibits acid hydrolysis of the siloxane bonds,
even under extremes of high temperature
and low pH.
PFP 90 3.6 2 to 8 60 Highly retentive and selective for protonated basic
analytes and molecules containing aromatic moities. propyl spacer.
PhenylHexyl 90 3.0 2 to 9 60 Alternative selectivity to alkyl bonded phases, Base-deactivated for good peak shapes
recommended for aromatic groups. Compatible when separating basic compounds. Hexyl
with highly aqueous mobile phases to facilitate the
retention and separation of polar compounds. phenyl ring to facilitate π-π interactions
with solutes.
RP-Amide 90 3.0 2 to 9 60 Base-deactivated phase with a polar group
retention and selectivity of acids, for symmetrical within the alkyl bonded phase. Provides
peak shapes of bases, zwitterions and other polar unique selectivity and a high level of base
compounds. deactivation while reducing or eliminating
the need for mobile phase additives.

* Maximum pressure 9,000 p.s.i. for all columns. All particle sizes are 2.7 µm.
**Not end-capped. All others end-capped.

16 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 BROWNLEE SPP OPTIMIZATION KITS

THE TECHNOLOGY
YOU NEED TO SEPARATE
FROM THE PACK
Brownlee Instrument Optimizaton Kits
Brownlee SPP columns can be used to enhance the performance
of virtually any PerkinElmer, Agilent, or other manufacturer’s HPLC
or UHPLC system. Our SPP columns deliver resolution and speed
similar to sub-2 μm UHPLC columns, but generate only 40% to
50% of the backpressure. By combining high ef
backpressure, Brownlee SPP columns make it possible to get
“UHPLC-like” performance from your HPLC system.
• Generate UHPLC like separations on your standard HPLC system
PerkinElmer has created, tested and made the following
• Achieve ultra fast performanc iency on your
PerkinElmer and Agilent optimization kits available. Our
UHPLC system
OneSource Service Engineers ar
• Run samples in a fraction of the time
manufacturer instruments and can help optimize your
instrument’ • Experience signi vings through faster method development,
column longevity and use of less mobile phase solvent
PerkinElmer Optimization Kits
Description Part No.
Series 200 LC or Flexar UHPLC systems N2920191
with UV/Vis detector
Series 200 LC or Flexar UHPLC systems N2920193
with PDA detector

Agilent Optimization Kits

Description Part No.
Extra column volume (ECV) - STD ZECVSTDVWD
Autosampler and Variable Wavelength
Detector (VWD) Optimization Kit
ECV - STD Well Plate Autosampler and ZECVSTDWPDADMWD
Diode Array Detector/Multiple Wave-
length Detector (DAD/MWD)
ECV - STD Autosampler and DAD/MWD ZECVSTDDADMWD
ECV - STD Well Plate Autosampler ZECVSTDWPVWD
and VWD
Ultra Low ECV - STD Autosampler ZULECVSTDVWD
and VWD
Ultra Low ECV - STD Well Plate ZULECVSTDWPDADMWD
Autosampler and DAD/MWD
Ultra Low ECV - STD Autosampler and ZULECVSTDDADMWD
DAD/MWD
Ultra Low ECV - STD Well Plate ZULECVSTDWPVWD
Autosampler and VWD

www.perkinelmer.com/supplies 17
 SPP

Performance For Every Phase Diameter/Length Part No. Diameter/Length Part No.
4.6 x 30 mm N9308430 4.6 x 50 mm N9308493
Brownlee SPP 2.7 µm Columns
4.6 x 50 mm N9308431 4.6 x 75 mm N9308494
Diameter/Length Part No. 4.6 x 75 mm N9308432 4.6 x 100 mm N9308495
C18 4.6 x 100 mm N9308433 4.6 x 150 mm N9308496
2.1 x 20 mm N9308400 4.6 x 150 mm N9308434 RP-Amide
2.1 x 30 mm N9308401 PFP 2.1 x 20 mm N9308497
2.1 x 50 mm N9308402 2.1 x 20 mm N9308465 2.1 x 30 mm N9308498
2.1 x 75 mm N9308403 2.1 x 30 mm N9308466 2.1 x 50 mm N9308499
2.1 x 100 mm N9308404 2.1 x 50 mm N9308467 2.1 x 75 mm N9308500
2.1 x 150 mm N9308405 2.1 x 75 mm N9308468 2.1 x 100 mm N9308501
3.0 x 20 mm N9308406 2.1 x 100 mm N9308469 2.1 x 150 mm N9308502
3.0 x 30 mm N9308407 2.1 x 150 mm N9308470 3.0 x 30 mm N9308503
3.0 x 50 mm N9308408 3.0 x 30 mm N9308471 3.0 x 50 mm N9308504
3.0 x 75 mm N9308409 3.0 x 50 mm N9308472 3.0 x 75 mm N9308505
3.0 x 100 mm N9308410 3.0 x 75 mm N9308473 3.0 x 100 mm N9308506
3.0 x 150 mm N9308411 3.0 x 100 mm N9308474 3.0 x 150 mm N9308507
4.6 x 20 mm N9308412 3.0 x 150 mm N9308475 4.6 x 30 mm N9308508
4.6 x 30 mm N9308413 4.6 x 30 mm N9308476 4.6 x 50 mm N9308509
4.6 x 50 mm N9308414 4.6 x 50 mm N9308477 4.6 x 75 mm N9308510
4.6 x 75 mm N9308415 4.6 x 75 mm N9308478 4.6 x 100 mm N9308511
4.6 x 100 mm N9308416 4.6 x 100 mm N9308479 4.6 x 150 mm N9308512
4.6 X 150 mm N9308417 4.6 x 150 mm N9308480 C18 Guard Column Packs
Peptide ES-C18 HILIC 2.1 x 5 mm, 3 pk N9308513
2.1 x 30 mm N9308450 2.1 x 30 mm N9308435 3.0 x 5 mm, 3 pk N9308514
2.1 x 50 mm N9308451 2.1 x 50 mm N9308436 4.6 x 5 mm, 3 pk N9308515
2.1 x 75 mm N9308452 2.1 x 75 mm N9308437 C8 Guard Column Packs
2.1 x 100 mm N9308453 2.1 x 100 mm N9308438 2.1 x 5 mm, 3 pk N9308522
2.1 x 150 mm N9308454 2.1 x 150 mm N9308439 3.0 x 5 mm, 3 pk N9308523
3.0 x 30 mm N9308455 3.0 x 30 mm N9308440 4.6 x 5 mm, 3 pk N9308524
3.0 x 50 mm N9308456 3.0 x 50 mm N9308441 HILIC Guard Column Packs
3.0 x 75 mm N9308457 3.0 x 75 mm N9308442 2.1 x 5 mm, 3 pk N9308525
3.0 x 100 mm N9308458 3.0 x 100 mm N9308443 3.0 x 5 mm, 3 pk N9308526
3.0 x 150 mm N9308459 3.0 x 150 mm N9308444 4.6 x 5 mm, 3 pk N9308527
4.6 x 30 mm N9308460 4.6 x 30 mm N9308445 Peptide ES-C18 Guard Column Packs
4.6 x 50 mm N9308461 4.6 x 50 mm N9308446 2.1 x 5 mm, 3 pk N9308528
4.6 x 75 mm N9308462 4.6 x 75 mm N9308447 3.0 x 5 mm, 3 pk N9308529
4.6 x 100 mm N9308463 4.6 x 100 mm N9308448 4.6 x 5 mm, 3 pk N9308530
4.6 x 150 mm N9308464 4.6 x 150 mm N9308449 PFP Guard Column Packs
C8 Phenyl-Hexyl 2.1 x 5 mm, 3 pk N9308531
2.1 x 20 mm N9308418 2.1 x 20 mm N9308481 3.0 x 5 mm, 3 pk N9308532
2.1 x 30 mm N9308419 2.1 x 30 mm N9308482 4.6 x 5 mm, 3 pk N9308533
2.1 x 50 mm N9308420 2.1 x 50 mm N9308483 Phenyl-Hexyl Guard Column Packs
2.1 x 75 mm N9308421 2.1 x 75 mm N9308484 2.1 x 5 mm, 3 pk N9308519
2.1 x 100 mm N9308422 2.1 x 100 mm N9308485 3.0 x 5 mm, 3 pk N9308520
2.1 x 150 mm N9308423 2.1 x 150 mm N9308486 4.6 x 5 mm, 3 pk N9308521
3.0 x 30 mm N9308424 3.0 x 30 mm N9308487 RP-Amide Guard Column Packs
3.0 x 50 mm N9308425 3.0 x 50 mm N9308488 2.1 x 5 mm, 3 pk N9308516
3.0 x 75 mm N9308426 3.0 x 75 mm N9308489 3.0 x 5 mm, 3 pk N9308517
3.0 x 100 mm N9308427 3.0 x 100 mm N9308490 4.6 x 5 mm, 3 pk N9308518
3.0 x 150 mm N9308428 3.0 x 150 mm N9308491 Guard Column Holder
4.6 x 20 mm N9308429 4.6 x 30 mm N9308492 N9308534

18 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 UHPLC

BROWNLEE UHPLC 15,000 PSI COLUMNS

Brownlee Columns for HRes™ Fast-LC Description Length Part No.

(1.9 μm Particle Size) Brownlee HRes Biphenyl 30 mm N9303911

Brownlee HRes Biphenyl 50 mm N9303912
Recent developments in LC column technology have now made
Brownlee HRes Biphenyl 100 mm N9303913
the use of sub 3 μm particle packing materials practical. These
smaller particle size materials allow higher mobile phase linear Brownlee HRes PFP Propyl 30 mm N9303914
velocities to be used, without the decrease in separation ef ciency
Brownlee HRes PFP Propyl 50 mm N9303915
experienced with conventional larger particle size packings. This
Brownlee HRes PFP Propyl 100 mm N9303916
translates into higher throughput and higher productivity. To
conserve mobile phase, these sub 3 μm column materials are Brownlee HRes Aqueous C18 30 mm N9303917
typically packed into 2.1 mm i.d. columns so that w rates
Brownlee HRes Aqueous C18 50 mm N9303918
(mobile phase volume) are kept low. However, smaller sized
Brownlee HRes Aqueous C18 100 mm N9303919
packings result in elevated operating pressures. Technological
advances in pump and injector designs have also kept pace, and Brownlee HRes IBD 30 mm N9303920
these columns can be used at pressures as high as 10,000 psi Brownlee HRes IBD 50 mm N9303921
with the PerkinElmer Series 275 or up to 15,000 psi using
Brownlee HRes IBD 100 mm N9303922
FX15 LC systems.
The rugged construction of this new line of small particle Brownlee HRes Analytical C18 30 mm N9303852
columns for high pressure application yield excellent longevity Brownlee HRes Analytical C18 50 mm N9303853
and reproducibility. These packings exhibit an exceptionally tight Brownlee HRes Analytical C18 100 mm N9303854
particle size distribution for excellent resolving power and are
packed at elevated pressures to assure column stability even at Brownlee HRes Analytical DB Silica 30 mm N9303970
high pressure applications. Each column is tested to ensure the Brownlee HRes Analytical DB Silica 50 mm N9303971
highest level of quality and ef ciency in high pressure LC testing. Brownlee HRes Analytical DB Silica 100 mm N9303972

Brownlee HRes Analytical DB Cyano 30 mm N9303973

Features and Be
Brownlee HRes Analytical DB Cyano 50 mm N9303974
• Sub 3 μm particle technology and 2.1 mm i.d. for higher
separat iency Brownlee HRes Analytical DB Cyano 100 mm N9303975

• Packed to withstand higher operating pressures Brownlee HRes Analytical DB PAH 50 mm N9303995
• Wide choice of stationary phases Brownlee HRes Analytical DB PAH 100 mm N9303996

End Carbon
Phase Cap? Load Applications Chromatographic Properties
C18 Y 11 Hydrophobic C18 Phase suitable for analyses of a wide range of Highly base-deactivated spherical silica. Monomeric C18 bonding.
compounds, from acidic through slightly basic.
Aqueous N 6 Ideal for applications that require highly aqueous mobile phases, Highly selective phase for polar analytes. Compatible with highly aqueous (up to
C18 such as organic acids and water-soluble vitamins. 100%) mobile phases.
Biphenyl Y 8 Excellent choice for the analysis of steroids, tetracyclines, drug Highly base-deactivated spherical silica. Unique reversed phase material that displays
metabolites, and other compounds that contain some degree both increased retention and selectivity for aromatic and/or unsaturated compounds
of unsaturation. when compared to conventional alkyl and phenyl phases.
PFPP Y 6 Exhibits excellent peak shapes for a wide range of compounds, Highly base-deactivated spherical silica.
including nucleosides, nucleotides, and halogenated compounds. propyl spacer.
IBD N - Intrinsically base-deactivated (IBD) phases, with a polar group within, or intrinsic to,
selectivity of acids, for symmetrical peak shapes of bases and for the alkyl bonded phase. Provides unique selectivity and a high level of base deacti-
the analysis of zwitterions and other polar compounds. vation while reducing or eliminating the need for mobile phase additives.
Silica - - Normal phase mode of separation. Highly base-deactivated spherical silica.
C8 Y 7 Superior general purpose C8 for non-basic analytes. Provides shorter retention times for hydrophobic compounds than C18.
PAH Y - Maximum resolution of polycyclic aromatic hydrocarbons. Proprietary stationary phase; resloves 16 PAHs in US EPA Method 610.
Cyano Y 4 Superior general purpose cyano for weakly-basic analytes. Used in More rugged than bare silica for normal phase analyses.
either normal or reversed phase analyses.
Pore Size A = 140, pH Range = 2.5-8, Temp Limit = 80°C, Particle Size = 1.9 µm

www.perkinelmer.com/supplies 19
 CONVENTIONAL COLUMNS

NPC is useful for functional group analysis, isomeric separations,

puri cation of organics, and sample cleanup.
Conventional columns do not require MPLC holders.
Ion-Exchange Chromatography (IEC) utilizes bonded ionic
Brownlee Conventional Columns groups to separate ionic analytes. Retention is based on the
af ity of various analyte ions for the stationary phase and various
Conventional Brownlee Columns (non-cartridge) use standard
mobile phase parameters (e.g., pH, ionic strength).
compression end ttings compatible with all HPLC
instrumentation. These columns do not require MPLC holders. Brownlee Analytical
This is PerkinElmer’s all-purpose HPLC column line for conventional
Features and Be as well as high speed LC separations. The (110 Å) silica is equivalent
•E ings compatible with HPLC systems from any vendor to the Hypersil. Columns are available in 3 or 5 µ and lengths ranging
from 30–250 mm, in 2.1 and 4.6 i.d. The Brownlee Analytical family
• Use with NewGuard system (line connection) for even longer
includes Amino, C18, C8, Cyano, Phenyl, Silica, and PAH phases.
column life
• Each column is individually tested Brownlee Aquapore®
This is a wide-pore (300 Å, 7, and 20 µm), silica-based support
suitable for the separation of large biopolymers.

Brownlee Bio
This material is wide-pore silica (300 Å) that is excellent for peptide
250 mm, 100 mm Microbore Columns (1.0 mm i.d.)
and protein separation. Columns are available in 5 µ and lengths
ranging from 30–250 mm, in 2.1 and 4.6 i.d. in C8 and C18 phases.
Brownlee Microbore HPLC Columns
Microbore columns (1.0 mm i.d.) are ideal for the analysis of Brownlee CHOICE™
samples of limited availability (e.g., micropuri cation of picomole This line of columns is made of (60 Å) silica which results in large
levels of proteins/peptides). Reducing the column diameter from surface area, high carbon loads, and high retention compounds.
the standard 4.6 mm to 1.0 mm increases the mass sensitivity by 20 Columns are available in 3 or 5 µm and lengths ranging from
times. Sample components can be collected in small elution volumes 30–250 mm, in 2.1 and 4.6 i.d. The Brownlee Choice family
(1–50 µL) for further sequence or mass analysis. Microbore offers includes the Basix, C18, Organic Acids, and PFP Propyl phases.
major bene ts in pharmacokinetic assays and LC/MS applications.
The typical w rate range is 10–100 µL/min., therefore specialized Brownlee Spheri-5®
HPLC systems may be required for optimum column performance. This is a small-pore (80 Å, 5 µm), silica based sorbent for separating
small molecules. Both monofunctional (comb-pre) and polyfunctional
Features and Be (polymerized loop-type) C8 and C18 sorbents available.
• Increase sensitivity and decrease solvent consumption by a factor of Brownlee Validated™
20 (compared with 4.6 mm i.d.)
These columns are made on high-purity, Type B silica with
• Reduce sample size for pharmacokinetic assays inherent low silanophilic activity. It exhibits excellent peak shape
• Be er compatibility with LC/MS interfaces for a wide range of compounds while also having a wide number
of phases. The Brownlee Validated columns are the best choice
• Each column is individually tested
for pharmaceutical applications that deal with a lot of basic or
multi-functional compounds. Columns are available in 3 or 5 µ
and lengths ranging from 30–250 mm, in 2.1 and 4.6 i.d., and
Modes of Separation include Amino, C18, C8, Carbamate, Cyano, IBD (intrinsically
Reversed-Phase Chromatography (RPC) is the most popular base-deactivated), PFP (penta rophenyl) and phenyl phases.
HPLC mode and is used in 60–80% of all applications. In RPC,
hydrophobic groups (C18, C8, or C4) bonded to a silica support Guard Columns and Cartridges
are used together with a mobile phase of water and organic To protect your column investment, we offer a complete line of
solvent (e.g. methanol, acetonitrile). guard columns in our direct connect guard cartridges. These can
Polar Phase (Normal Phase) Chromatography (NPC) uses a be attached to any column offered above. You can easily select the
polar stationary phase (unbonded silica, bonded amino or cyano phase type to the appropriate guard column packing to ensure a
group) together with a non-polar mobile phase (e.g., hexane). correct silica match.

20 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 CONVENTIONAL COLUMNS

Brownlee Columns
Choose only genuine PerkinElmer Brownlee™ columns for the
best separation results. Six different column types to meet all your
analytical needs.

Features and Be
• Each column individually tested and certi te provided
•E ings compatible with a variety of HPLC systems
• Do not require MPLC holders

Conventional Columns Conventional columns do not require MPLC holders.

Column Functionality Pore Diameter Å % Carbon End Cap Applications

Analytical Amino 110 2 No Excellent for carbohydrate analysis
Analytical C18 & C8 110 13 Yes Superior for non-basic analytes
Analytical Cyano 110 4 Yes General purpose for normal or reversed-phase
Analytical Biphenyl 110 – Yes U.S. EPA Method 8330
Analytical Phenyl 110 6 Yes General purpose for neutral analytes
Analytical Silica 110 – – Ideal for polar analytes
Analytical PAH 110 – Yes Maximum resolution of polynuclear aromatic hydrocarbons
Aquapore ®
AX-300 300 – – Used for ion exchange chromatography
Aquapore Octyl C8 300 – – Used for reversed-phase chromatography
Aquapore ODS C18 300 – – Silica based support used for the separation of large biopolymers
Bio C18 300 6 Yes High molecular weight compounds and proteins
Bio C8 300 – – High molecular weight compounds and proteins
CHOICE ™
Basix 60 12 Yes Ideal for LC/MS. Excellent for amine-containing compounds and
basic pharmaceuticals
CHOICE C18 60 27 Yes Excellent for explosives or steroids
CHOICE Biphenyl 60 23 Yes Highly retentive and selective phase for aromatic compounds
CHOICE Organic Acids 60 – No Used for challenging organic acids
CHOICE PFP Propyl 60 17 Yes Ideal for MS, ELSD, or NPD detection of nucleosides, nucleotides,
purines, pyrimidines or halogenated compounds
Spheri-5® C8, C18, C18 ODS and Silica 80 – – Excellent for separating small molecules both
non-functional and polyfunctional
Validated™ Amino 100 2 No Ideal for carbohydrates
Validated C1 100 5 – Least retentive reversed-phase hydrocarbon packing
Validated C4 100 9 Yes High bonding coverage and base deactivation
Validated Aqueous C18 100 15 No Excellent for highly water-soluble or poorly organic-soluble compounds
Validated C18 100 20 Yes Used for the detection of anilines, barbiturates, carbonyls, fat-soluble
vitamins, fatty acids, glycerides, phthalates
Validated C8 100 12 Yes Less hydrophobic retention than C18
Validated Carbamate 100 – – Fast analysis of carbamates
Validated Cyano 100 8 Yes Basic pharmaceuticals and steroids
Validated IBD 100 12 No Unique separation for acids, bases, zwitterions and other
polar compounds
Validated PFP 100 7 Yes Ideal for taxors and precursors or halogenated compounds
Validated Phenyl 100 10 Yes Separation of fatty acids, polynuclear aromatic hydrocarbons,
purines and pyrimidines
Validated Quat 100 – – High purity, base deactivated reversed-phase packing

www.perkinelmer.com/supplies 21
 CONVENTIONAL COLUMNS

Brownlee Analytical Conventional Columns

Brownlee Analytical Amino
Ideal column for mono and disaccharide analyses. Most popular
amino-based stationary phase. Routine analysis of simple sugars,
using isocratic elution and a refractive index detector or an
evaporative light scattering detector.
pH range: 2.5 to 7.5 - Temperature limit: 80° C
Pore size: 110 Å - Carbon load: 2% - End Cap: No

Phase Length ID Particle Size Part No. Brownlee Analytical Cyano

Amino 50 mm 4.6 mm 3 µm N9303502
Suitable for analyses of a wide range of compounds from acidic
Amino 100 mm 4.6 mm 3 µm N9303500
through slightly basic. Highly base-deactivated spherical silica.
Amino 150 mm 4.6 mm 3 µm N9303501
pH range: 2.5 to 7.5 - Temperature limit: 80° C
Amino 50 mm 4.6 mm 5 µm N9303506
Pore size: 110 Å - Carbon load: 4% - End Cap: Yes
Amino 100 mm 4.6 mm 5 µm N9303503
Amino 150 mm 4.6 mm 5 µm N9303504 Phase Length ID Particle Size Part No.
Amino 250 mm 4.6 mm 5 µm N9303505 Cyano 150 mm 4.6 mm 5 µm N9303522
Cyano 250 mm 4.6 mm 5 µm N9303523
Brownlee Analytical C8
Shorter retention times for hydrophobic compounds.
Brownlee Analytical PAH
Reliable performance with symmetrical peak shapes Speci cally designed for challenging analyses of polynuclear
for neutral to acidic compounds. aromatic hydrocarbons.
pH range: 2.5 to 7.5 - Temperature limit: 80° C pH range: 2.5 to 7.5 - Temperature limit: 80° C
Pore size: 100 Å - Carbon load: 7% - End Cap: Yes Pore size: 110 Å - End Cap: Yes

Phase Length ID Particle Size Part No. Phase Length ID Particle Size Part No.
C8 30 mm 4.6 mm 5 µm N9303521 PAH 100 mm 4.6 mm 5 µm N9303527
C8 100 mm 4.6 mm 5 µm N9303515 PAH 150 mm 3.2 mm 5 µm N9303430
C8 150 mm 4.0 mm 5 µm N9303516 PAH 150 mm 4.6 mm 5 µm N9303529
C8 150 mm 4.6 mm 5 µm N9303517 PAH 200 mm 4.6 mm 5 µm N9303528
C8 200 mm 4.6 mm 5 µm N9303518 PAH 250 mm 2.1 mm 5 µm N9303530
C8 250 mm 4.0 mm 5 µm N9303519 PAH 250 mm 4.6 mm 5 µm N9303531
C8 250 mm 4.6 mm 5 µm N9303520
Brownlee Analytical Phenyl
Brownlee Analytical C18 Unique selectivity especially for aromatic compounds.
Suitable for a wide range of acidic to neutral hydrophobic pH range: 2.5 to 7.5 - Temperature limit: 80° C
compounds. Excellent all purpose column. Carbon load: 6% - Pore size: 110 Å - End Cap: Yes
pH range: 2.5 to 7.5 - Temperature limit: 80° C
Phase Length ID Particle Size Part No.
Pore size: 110 Å - Carbon load: 13% - End Cap: Yes
Phenyl 150 mm 4.6 mm 5 µm N9303524
Phase Length ID Particle Size Part No.
C18 30 mm 4.6 mm 3 µm N9303509 Brownlee Analytical Silica
C18 50 mm 4.6 mm 3 µm N9303510 Good general purpose packing for normal phase separations.
C18 100 mm 4.6 mm 3 µm N9303507
pH range: 2.5 to 7.5 - Temperature limit: 80° C
C18 150 mm 4.6 mm 3 µm N9303508
Pore size: 110 Å - End Cap: No
C18 100 mm 4.0 mm 5 µm N9303511
C18 100 mm 4.6 mm 5 µm N9303512 Phase Length ID Particle Size Part No.

C18 150 mm 4.6 mm 5 µm N9303513 Silica 150 mm 4.6 mm 5 µm N9303525

C18 250 mm 4.6 mm 5 µm N9303514 Silica 250 mm 4.6 mm 5 µm N9303526

22 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 CONVENTIONAL COLUMNS

Brownlee Aquapore Conventional Columns Brownlee Bio Conventional Columns

Brownlee Aquapore AX-300 Brownlee Bio C8
Used for Ion Exchange chromatography. Wide-pore HPLC Column. High proportion of pore/surface area
available for large molecule separations.
Pore Size: 300 Å
Pore Size: 300 Å - Carbon Load: 6% - End Cap: Yes
Phase Length ID Particle Size Part No.
AX-300 250 mm 4.6 mm 7 µm 07120040 Phase Length ID Particle Size Part No.
C8 50 mm 2.1 mm 5 µm N9303664
Brownlee Aquapore Octyl C8 C8 50 mm 4.6 mm 5 µm N9303665

Used for Reversed Phase chromatography. C8 100 mm 2.1 mm 5 µm N9303656

C8 100 mm 4.6 mm 5 µm N9303657
Pore Size: 300 Å
C8 150 mm 2.1 mm 5 µm N9303658
Phase Length ID Particle Size Part No. C8 150 mm 4.6 mm 5 µm N9303659
C8 100 mm 1.0 mm 7 µm 07120096 C8 200 mm 2.1 mm 5 µm N9303660
C8 250 mm 1.0 mm 7 µm 07120097 C8 200 mm 4.6 mm 5 µm N9303661
C8 250 mm 4.6 mm 7 µm 07120033 C8 250 mm 2.1 mm 5 µm N9303662
C8 250 mm 4.6 mm 5 µm N9303663
Brownlee Aquapore ODS C18
Silica-based support suitable for the separation of large Brownlee Bio C18
biopolymers. Microbore columns increase sensitivity, reduce Excellent for separating peptides or proteins. Wide-pore
sample size, and have better compatibility with LC/MS interfaces. HPLC Column.
Pore Size: 300 Å Pore Size: 300 Å - Carbon Load: 6% - End Cap: Yes
Phase Length ID Particle Size Part No. Phase Length ID Particle Size Part No.
C18 100 mm 1.0 mm 7 µm 07120099 C18 50 mm 2.1 mm 5 µm N9303654
C18 250 mm 4.6 mm 7 µm 07120073 C18 50 mm 4.6 mm 5 µm N9303655
C18 100 mm 2.1 mm 5 µm N9303646
C18 100 mm 4.6 mm 5 µm N9303647
C18 150 mm 2.1 mm 5 µm N9303648
C18 150 mm 4.6 mm 5 µm N9303649
C18 200 mm 2.1 mm 5 µm N9303650
C18 200 mm 4.6 mm 5 µm N9303651
C18 250 mm 2.1 mm 5 µm N9303652
C18 250 mm 4.6 mm 5 µm N9303653

www.perkinelmer.com/supplies 23
 CONVENTIONAL COLUMNS

Brownlee CHOICE Conventional Columns Brownlee CHOICE Propyl

Brownlee CHOICE Basix Highly retentive for basic analytes. Excellent separation of
nucleosides, nucleotides, purines, pyrimidines halogenated
Excellent choice for analytes containing amine
compounds, beta blockers and tricyclic antidepressants.
group functionality.
pH range: 2.5 to 7.5 - Temperature limit: 80° C -
pH range: 2.5 to 7.5 - Temperature limit: 80° C -
Pore Size: 60 Å - Carbon Load: 17% - End Cap: Yes
Pore Size: 60 Å - Carbon Load: 12% - End Cap: Yes
Phase Length ID Particle Size Part No.
Phase Length ID Particle Size Part No.
PFP Propyl 30 mm 2.1 mm 5 µm N9303640
Basix 50 mm 2.1 mm 5 µm N9303615
PFP Propyl 50 mm 2.1 mm 5 µm N9303641
Basix 50 mm 4.6 mm 5 µm N9303616
PFP Propyl 50 mm 4.6 mm 5 µm N9303642
Basix 100 mm 2.1 mm 5 µm N9303613
PFP Propyl 100 mm 2.1 mm 5 µm N9303638
Basix 100 mm 4.6 mm 5 µm N9303614
PFP Propyl 100 mm 4.6 mm 5 µm N9303639
Basix 150 mm 2.1 mm 5 µm N9303617
PFP Propyl 150 mm 2.1 mm 5 µm N9303643
Basix 150 mm 4.6 mm 5 µm N9303618
PFP Propyl 150 mm 4.6 mm 5 µm N9303644
Basix 250 mm 4.6 mm 5 µm N9303619
PFP Propyl 250 mm 4.6 mm 5 µm N9303645

Brownlee CHOICE C18

Excellent choice for LC/MS. Highly retentive phase for Brownlee Spheri-5 Conventional Columns
hydrophobic and slightly polar analytes.
Brownlee Spheri-5®
pH range: 2.5 to 7.5 - Temperature limit: 80° C -
Small pore silica-based sorbent for separating small
Pore Size: 60 Å - Carbon Load: 27% - End Cap: Yes
molecules both non-functional and polyfunctional
Phase Length ID Particle Size Part No. C8 and C18 are available.
C18 50 mm 2.1 mm 5 µm N9303626
Length: 250 mm - ID: 4.6 mm - Pore Size: 80 Å
C18 100 mm 2.1 mm 5 µm N9303625
Phase Length ID Particle Size Part No.
C18 150 mm 2.1 mm 5 µm N9303627
C2 250 mm 4.6 mm 10 µm 07120005
C18 150 mm 4.6 mm 5 µm N9303628
C8 250 mm 4.6 mm 5 µm 07120012
C18 250 mm 2.1 mm 5 µm N9303629
C18 250 mm 4.6 mm 5 µm 07120016
C18 250 mm 4.6 mm 5 µm N9303630
C18 250 mm 4.6 mm 10 µm 07120001

Brownlee CHOICE Organic Acids C18 ODS 250 mm 4.6 mm 5 µm 07120019

Silica 250 mm 4.6 mm 5 µm 07120023
Enhanced retention and selectivity for polar organic acids.
Retention is stable and reproducible even with 100%
aqueous mobile phase speci ed in the AOAC method.
pH range: 2.5 to 7.5 - Temperature limit: 80° C -
Pore Size: 60 Å - End Cap: No

Phase Length ID Particle Size Part No.

Organic Acids 150 mm 4.6 mm 5 µm N9303631
Organic Acids 250 mm 2.1 mm 5 µm N9303632
Organic Acids 250 mm 4.6 mm 5 µm N9303633
Organic Acids 300 mm 4.6 mm 5 µm N9303634

24 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 CONVENTIONAL COLUMNS

Brownlee Validated Conventional Columns Brownlee Validated C4

Brownlee Validated™ Amino High bonding coverage and base deactivation. Less retention than
C18 or C8 phases.
Good for normal phase analytes of mono- and disaccharides or
similar compounds. pH range: 2.5 to 7.5 - Temperature limit: 80° C -
Pore Size: 100 Å - Carbon Load: 9% - End Cap: Yes
pH range: 2.5 to 7.5 - Temperature limit: 80° C -
Particle Size: 5 µm - Pore Size: 100 Å - Carbon Load: 2% Phase Length ID Particle Size Part No.
C4 150 mm 4.6 mm 5 µm N9303720
Phase Length ID Particle Size Part No.
C4 250 mm 4.6 mm 5 µm N9303721
Amino 100 mm 2.1 mm 5 µm N9303532
Amino 150 mm 2.1 mm 5 µm N9303533
Brownlee Validated C8
Amino 150 mm 4.6 mm 5 µm N9303534
Retentive, high purity base-deactivated reversed phase packing.
Amino 200 mm 4.6 mm 5 µm N9303535
Amino 250 mm 4.6 mm 5 µm N9303536 pH range: 2.5 to 7.5 - Temperature limit: 80° C -
Pore Size: 80 Å - Carbon Load: 12% - End Cap: Yes
Brownlee Validated Aqueous C18 Phase Length ID Particle Size Part No.
Highly base deactivated. Compatible with highly aqueous C8 100 mm 2.1 mm 5 µm N9303564
mobile phases. C8 100 mm 4.6 mm 5 µm N9303565

pH range: 2.5 to 7.5 - Temperature limit: 80° C - C8 150 mm 2.1 mm 5 µm N9303566

Pore Size: 100 Å - Carbon Load: 15% - End Cap: No C8 150 mm 4.6 mm 5 µm N9303567
C8 250 mm 4.6 mm 5 µm N9303568
Phase Length ID Particle Size Part No.
Aqueous C18 30 mm 2.1 mm 3 µm N9303540
Brownlee Validated C18
Aqueous C18 50 mm 2.1 mm 3 µm N9303541
Aqueous C18 50 mm 4.6 mm 3 µm N9303542
Excellent general purpose phase column.
Retentive high purity packing.
Aqueous C18 100 mm 2.1 mm 3 µm N9303537
Aqueous C18 100 mm 4.6 mm 3 µm N9303538 pH range: 2.5 to 7.5 - Temperature limit: 80° C -
Aqueous C18 150 mm 2.1 mm 3 µm N9303539 Pore Size: 100 Å - Carbon Load: 20% - End Cap: Yes
Aqueous C18 30 mm 4.6 mm 5 µm N9303550 Phase Length ID Particle Size Part No.
Aqueous C18 100 mm 2.1 mm 5 µm N9303543 C18 100 mm 2.1 mm 3 µm N9303551
Aqueous C18 100 mm 4.6 mm 5 µm N9303544 C18 100 mm 4.6 mm 3 µm N9303552
Aqueous C18 150 mm 2.1 mm 5 µm N9303545 C18 50 mm 2.1 mm 5 µm N9303562
Aqueous C18 150 mm 4.6 mm 5 µm N9303546 C18 50 mm 4.6 mm 5 µm N9303563
Aqueous C18 200 mm 4.6 mm 5 µm N9303547 C18 100 mm 2.1 mm 5 µm N9303553
Aqueous C18 250 mm 2.1 mm 5 µm N9303548 C18 100 mm 4.6 mm 5 µm N9303554
Aqueous C18 250 mm 4.6 mm 5 µm N9303549 C18 150 mm 2.1 mm 5 µm N9303556
C18 150 mm 4.0 mm 5 µm N9303557
Brownlee Validated C1 C18 150 mm 4.6 mm 5 µm N9303558
Least retentive reversed phase hydrocarbon packing. C18 200 mm 4.6 mm 5 µm N9303559

pH range: 2.5 to 7.5 - Temperature limit: 80° C - C18 250 mm 2.1 mm 5 µm N9303560

Pore Size: 100 Å - Carbon Load: 5% - End Cap: No C18 250 mm 4.6 mm 5 µm N9303561

Phase Length ID Particle Size Part No.

C1 150 mm 4.6 mm 5 µm N9303718
C1 250 mm 4.6 mm 5 µm N9303719

www.perkinelmer.com/supplies 25
 CONVENTIONAL COLUMNS

Brownlee Validated Conventional Columns Brownlee Validated IBD (Continued)

Brownlee Validated™ Carbamate Phase Length ID Particle Size Part No.

A unique stationary phase bonded to a 3µm silica speci cally IBD 30 mm 1.0 mm 5 µm N9303585

designed for carbamate analysis. This column is compatible IBD 30 mm 2.1 mm 5 µm N9303586
with rescence or LC/MS detection. Validated Carbamate IBD 30 mm 4.6 mm 5 µm N9303587
column has much faster analysis, which will signi cantly reduce IBD 50 mm 2.1 mm 5 µm N9303588
solvent usage. IBD 50 mm 4.6 mm 5 µm N9303589

pH range: 2.5 to 7.5 - Temperature limit: 80° C - Pore Size: 100 Å IBD 100 mm 2.1 mm 5 µm N9303579
IBD 100 mm 4.6 mm 5 µm N9303580
Phase Length ID Particle Size Part No.
IBD 150 mm 2.1 mm 5 µm N9303581
Carbamate 50 mm 4.6 mm 3 µm N9303570
IBD 150 mm 4.6 mm 5 µm N9303582
Carbamate 100 mm 4.6 mm 3 µm N9303569
IBD 200 mm 4.6 mm 5 µm N9303583
Carbamate 250 mm 4.6 mm 3 µm N9303571
IBD 250 mm 4.6 mm 5 µm N9303584

Brownlee Validated Cyano

Brownlee Validated PFP
High purity Cyano phase with few silanol sites. Less sensitive to
Unique selectivity for compounds containing organohalogens or
small amounts of water present in the mobile phase.
other basic functional groups.
pH range: 2.5 to 7.5 - Temperature limit: 80° C -
pH range: 2.5 to 7.5 - Temperature limit: 80° C -
Pore Size: 100 Å - Carbon Load: 10% - End Cap: Yes
Pore Size: 100 Å - Carbon Load: 7% - End Cap: Yes
Phase Length ID Particle Size Part No.
Phase Length ID Particle Size Part No.
Cyano 30 mm 4.6 mm 5 µm N9303578
PFP 50 mm 2.1 mm 5 µm N9303601
Cyano 100 mm 2.1 mm 5 µm N9303572
PFP 100 mm 4.6 mm 5 µm N9303597
Cyano 100 mm 4.6 mm 5 µm N9303573
PFP 150 mm 2.1 mm 5 µm N9303598
Cyano 150 mm 2.1 mm 5 µm N9303574
PFP 150 mm 4.6 mm 5 µm N9303599
Cyano 150 mm 4.6 mm 5 µm N9303575
PFP 250 mm 4.6 mm 5 µm N9303600
Cyano 200 mm 4.6 mm 5 µm N9303576
Cyano 250 mm 4.6 mm 5 µm N9303577
Brownlee Validated Phenyl

Brownlee Validated IBD High purity, highly retentive base-deactivated phase, especially
designed for aromatic analytes.
Unique selectivity and a high level of base-deactivation. Reduces or
eliminates the need for mobile phase additives. pH range: 2.5 to 7.5 - Temperature limit: 80° C -
Pore Size: 100 Å - Carbon Load: 10% - End Cap: Yes
pH range: 2.5 to 7.5 - Temperature limit: 80° C -
Pore Size: 100 Å - Carbon Load: 12% - End Cap: No Phase Length ID Particle Size Part No.
Phenyl 50 mm 1.0 mm 3 µm N9303604
Phase Length ID Particle Size Part No.
Phenyl 100 mm 2.1 mm 3 µm N9303602
IBD 30 mm 4.6 mm 3 µm N9303594
Phenyl 100 mm 4.6 mm 3 µm N9303603
IBD 50 mm 2.1 mm 3 µm N9303595
Phenyl 150 mm 2.1 mm 5 µm N9303605
IBD 50 mm 4.6 mm 3 µm N9303596
Phenyl 150 mm 4.6 mm 5 µm N9303606
IBD 100 mm 2.1 mm 3 µm N9303590
Phenyl 250 mm 4.6 mm 5 µm N9303607
IBD 100 mm 4.6 mm 3 µm N9303591
IBD 150 mm 2.1 mm 3 µm N9303592
IBD 150 mm 4.6 mm 3 µm N9303593

26 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 CONVENTIONAL COLUMNS

Guard Cartridges and Accessories Guard Cartridges Brownlee CHOICE

Guard Cartridge Accessories Pore Size: 60 Å - Pkg: 3

Description Part No. Phase Length ID Particle Size Part No.

N9303713 Basix 10 mm 2.1 mm 5 µm N9303666

N9303712 Basix 10 mm 4.0 mm 5 µm N9303667

N9303711 C18 10 mm 2.1 mm 5 µm N9303668

P N9303714 C18 10 mm 4.0 mm 5 µm N9303669

Replacement cap frits 2.0 mm, 2.0 µm N9303717 Organic Acids 10 mm 2.1 mm 5 µm N9303670

Replacement cap frits 4.0 mm, 0.5 µm N9303716 Organic Acids 10 mm 4.0 mm 5 µm N9303671

Replacement cap frits 4.0 mm, 2.0 µm N9303715 PFP Propyl 10 mm 2.1 mm 5 µm N9303672
PFP Propyl 10 mm 4.0 mm 5 µm N9303673

Guard Cartridges Brownlee Analytical

Guard Cartridges Brownlee Validated
Pkg: 3
Pore Size: 100 Å - Pkg: 3
Phase Length ID Particle Size Part No.
Amino 10 mm 2.1 mm 5 µm N9303674 Phase Length ID Particle Size Part No.

Amino 10 mm 4.0 mm 5 µm N9303675 Amino 10 mm 2.1 mm 5 µm N9303688

C18 10 mm 2.1 mm 5 µm N9303676 Amino 10 mm 4.0 mm 5 µm N9303689

C18 10 mm 4.0 mm 5 µm N9303677 Aqueous C18 10 mm 2.1 mm 5 µm N9303690

C8 10 mm 2.1 mm 5 µm N9303678 Aqueous C18 10 mm 4.0 mm 5 µm N9303691

C8 10 mm 4.0 mm 5 µm N9303679 C1 10 mm 4.0 mm 5 µm N9303722

Cyano 10 mm 2.1 mm 5 µm N9303680 C4 10 mm 4.0 mm 5 µm N9303723

Cyano 10 mm 4.0 mm 5 µm N9303681 C8 10 mm 2.1 mm 5 µm N9303695

PAH 10 mm 2.1 mm 5 µm N9303682 C8 10 mm 4.0 mm 5 µm N9303696

PAH 10 mm 4.0 mm 5 µm N9303683 C18 10 mm 2.1 mm 5 µm N9303693

Phenyl 10 mm 2.1 mm 5 µm N9303684 C18 10 mm 4.0 mm 5 µm N9303694

Phenyl 10 mm 4.0 mm 5 µm N9303685 C18 10 mm 4.0 mm 10 µm N9303692

Silica 10 mm 2.1 mm 5 µm N9303686 Carbamate 10 mm 2.1 mm 5 µm N9303697

Silica 10 mm 4.0 mm 5 µm N9303687 Carbamate 10 mm 4.0 mm 5 µm N9303698

Cyano 10 mm 2.1 mm 5 µm N9303699

Guard Cartridges Brownlee Bio Cyano 10 mm 4.0 mm 5 µm N9303700

IBD 10 mm 2.1 mm 5 µm N9303701
Pore Size: 300 Å - Pkg: 3
IBD 10 mm 4.0 mm 5 µm N9303702
Phase Length ID Particle Size Part No. PFP 10 mm 2.1 mm 5 µm N9303703
C18 10 mm 2.1 mm 5 µm N9303708 PFP 10 mm 4.0 mm 5 µm N9303704
C18 10 mm 4.0 mm 5 µm N9303707 Phenyl 10 mm 2.1 mm 5 µm N9303705
C8 10 mm 2.1 mm 5 µm N9303709 Phenyl 10 mm 4.0 mm 5 µm N9303706
C8 10 mm 4.0 mm 5 µm N9303710

www.perkinelmer.com/supplies 27
 BROWNLEE CARTRIDGE COLUMNS

Brownlee Sorbents
PerkinElmer Brownlee™ cartridge columns are offered in a wide
range of sorbent material. Choose the right sorbent for the
analysis and mode of separation being used.
Aquapore® – is a wide-pore, silica-based support suitable for the
separation of large biopolymers.
Spheri-5® – is a small-pore, silica-based solvent for separating
small molecules. Both monofunctional (comb-type) and
polyfunctional (polymerized loop-type) C8 and C18 sorbents
are available.
Polypore® – is a polymer-based (microporous, 10 µm) sorbent for
the analysis of organic acids and sugars.
Validated™ – is a small-pore, silica-based C18 sorbent for small
molecules, speci cally for consistent pharmaceutical assays.
Pecosphere™ – is a small-pore, silica-based sorbent for HPLC
of small molecules. CHOICE™ – is a small-pore, base deactivated silica-based,
Reduced Activity – is a small-pore, base deactivated silica-based, reversed-phase sorbent (C18) developed for LC/MS separations.
reversed-phase sorbent (C18 or C8) developed for HPLC of basic Peco HCODS – is especially suited for reversed-phase separation
analytes and pharmaceuticals. of proteins and peptides.
Brownlee Cartridge Columns
Column Functionality Pore Diameter Å % Carbon End Cap Applications
Aquapore AX-300 (anion exchange) 300 - - Silica-based support for larger anionic compounds
Aquapore OD-300 (C18) 300 10 Yes Silica-based support for larger compounds by reversed-phase
Aquapore RP-300 (C8) 300 5 Yes Same as above, but for slightly more polar compounds
Aquapore BU-300 (C4) 300 3 Yes Same as above, but for even somewhat more polar compounds
Choice C18 100 12 Yes F ation of basic compounds/pharmaceuticals
Choice C8 100 7 Yes F ation of basic compounds/pharmaceuticals
Choice PFPP (P 100 5 Yes F ation of basic drugs, especially for LC/MS
Pecosphere C18, monofunctional 80 11 Yes Reversed-phase column for fast separation of smaller compounds
Pecosphere CR C18 80 11 Yes Same as above, but for more basic compounds
Pecosphere CR C8 80 11 Yes Same as above, but, also, for slightly more polar compounds
Peco HCODS C18, polyfunctional 300 9 No Especially suited for reversed-phase separation of proteins and peptides
Polypore CA (calcium form), Mixed Mode Microporous - - Especially suited for separation of organic acids, as well as sugars
Polypore H (hydrogen form), Mixed Mode Microporous - - Especially suited for separation of sugars, as well as organic acids
Reduced Activity C18 80 12 Yes Reversed-phase sorbent geared for fast separation of basic
compounds/pharmaceuticals
Reduced Activity C8 80 5 Yes Same as above, but for slightly more polar compounds
Spheri-5 RP-18 (monofunctional C18) 80 11 Yes Reversed-phase separation of smaller compounds
Spheri-5 ODS (polyfunctional C18) 80 14 Yes Same as above, but with somewhat different selectivity
Spheri-5 RP-8 (monofunctional C8) 80 6 Yes Reversed-phase separation of more basic smaller compounds
Spheri-5 Phenyl (C5) 80 6 Yes Especially suited for hydrophobic interaction separation of biomolecules,
where the activity of the compound must be preserved
Spheri-5 Amino 80 3 No Normal-phase separation of smaller, relatively more polar compounds
Spheri-5 Cyano 80 4 No Same as above, but with somewhat different selectivity
Spheri-5 Silica 80 - - Same as above, but with different selectivity
Validated C18 100 18 Yes Reversed-phase separations in which very little variation in both
lot-to-lot and column-to-column variability can be tolerated
VeloSep RP-18 (C18) 100 14 Yes Reversed-phase column for high through-put and minimal solvent usage
VeloSep RP-8 (C8) 100 7 Yes Same as above, but for slightly more polar compounds

28 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 BROWNLEE CARTRIDGE COLUMNS

BROWNLEE™ ANALYTICAL CARTRIDGE COLUMNS FOR

CONVENTIONAL AND NARROWBORE HPLC
Analytical cartridges from the Brownlee line are available in
three lengths (30, 100 and 220 mm) and two internal diameters
(4.6 and 2.1 mm). The MPLC cartridge system allows direct
coupling of a cartridge to a guard without any dead-volume.
Changing cartridges is easy with the er-tight cartridge design,
which does not require any wrench tightening of the holder or
disconnecting of tubing from the LC system.

Features and Be
• Quality – manufactured under ISO-9001 certi d quality
control procedures
• Quick and easy cartridge change w ger-tight design
• Flexible – same reusable holder for 2.1 and 4.6 mm cartridges
Brownlee Columns and Holders
• Economical – less expensive than conventional columns
Brownlee VeloSep®
Brownlee CHOICE™ VeloSep columns are 3.2 mm i.d. cartridges packed with 3 µm
reversed-phase support for high throughput analysis and reduced
The CHOICE™ columns provide the speed and ef ciency
solvent consumption. VeloSep is packed with 3 µm C8 or C18
appropriate for LC/MS. Each column’s separation requirements
sorbents and available in 40 or 100 mm lengths.
vary depending on the system, sample and mass spectrometer.

Brownlee PECO Brownlee Validated

Brownlee Validated sorbent is derived from a neutral “Type B”
PerkinElmer pioneered the development of Fast HPLC and
silica with inherent low silanophobic activity. Each sorbent is tested
introduced the popular 3 x 3 column. This column is capable of
to meet stringent speci cations for the separation of basic, neutral
rapid analysis, as short as one to two minutes. The 83 mm long
and acidic test probes. A high-temperature bonding technique
3 x 8 column with > 10,000 plates can separate many complex
is coupled with an exhaustive end-capping procedure to ensure
mixtures in less than 10 minutes. Peco cartridges are available with
maximum coverage of the silica surface. A proprietary treatment
MPLC® holders. Three cartridge lengths (33, 83 and 150 mm) are
step is used to further eliminate any trace residual metals in the
available in 4.6 mm i.d.
silica that create active absorptive sites. This is a highly deactivated
column which yields excellent peak shape for dif cult base
Brownlee Preparative analytes, pharmaceuticals and small peptides.
Preparative columns are 10 mm i.d. cartridges, packed with
Aquapore® 20 µm, 300 Å sorbents. Save time with high-pressure NewGuard Columns
preparative runs up to 3000 psi and achieve high performance
NewGuard cartridges are small guard cartridges (15 mm x 3.2 mm
with the 20 µm, 300 Å sorbents. Typical sample capacity of the
i.d.) packed with 5 or 7 µm sorbents. NewGuard columns prolong
250 mm cartridge is 50 mg to 1 g of material; the typical w rate
column life 2 to 5 times by eliminating particulates, contaminants
is 4–10 mL/min.
and strongly bound sample components. They act as disposable,
replaceable heads of your analytical columns. NewGuard columns
are easily coupled to MPLC cartridges or conventional columns.

Brownlee MPLC/Peco Holders

MPLC cartridge system ensures leak-free operation up to 7,000
psi, with only er-tightening. The high-pressure seal in each end
assembly actually seals tighter as pressure increases. No wrenches
are required and there is no need to disconnect any tubing to your
LC system when changing a cartridge. MPLC cartridges can be
directly coupled to another using a union.

www.perkinelmer.com/supplies 29
 BROWNLEE CARTRIDGE COLUMNS

Extensive Range of Phases and Column Lengths

Brownlee MPLC Cartridge Columns
30 mm x 4.6 mm i.d.* 30 mm x 2.1 mm i.d.* 100 mm x 4.6 mm i.d.**
Ion Exchange 2 per package 2 per package 1 per package
Column Description Part No. Part No. Part No.
AX-300, Aquapore® 7 µm, 300 Å, Weak Anion 07110073 07110074 07110075
Polypore CA
®
10 µm, Polymeric, Calcium Form 07110091 07110093
Polypore H 10 µm, Polymeric, Hydrogen Form 07110085 07110087
30 mm x 4.6 mm i.d.* 30 mm x 2.1 mm i.d.* 100 mm x 4.6 mm i.d.**
Polar Phase 2 per package 2 per package 1 per package
Column Description Part No. Part No. Part No.
Cyano, Spheri-5 Cyanopropyl, 5 µm, 80 Å 07110043 07110045
Silica, Spheri-5 5 µm, 80 Å 07110031 07110032 07110033
Silica, Spheri-10 10 µm, 80 Å 07110145
30 mm x 4.6 mm i.d.* 30 mm x 2.1 mm i.d.* 100 mm x 4.6 mm i.d.**
Reversed Phase 2 per package 2 per package 1 per package
Column Description Part No. Part No. Part No.
Cyano, Spheri-5 Cyanopropyl, 5 µm, 80 Å 07110043 07110045
OD-300, Aquapore ODS C-18, 7 µm, 300 Å 07110235 07110234 07110232
ODS, Spheri-5 C-18, Polyfunctional, 5 µm, 80 Å 07110019 07110020 07110021
Phenyl, Spheri-5 Phenyl, 5 µm, 80 Å 07110025 07110027
RP-8, Spheri-5 C-8, Monofunctional, 5 µm, 80 Å 07110001 07110002 07110003
RP-18, Spheri-5 C-18, Monofunctional, 5 µm, 80 Å 07110013 07110014 07110015
RP-300, Aquapore Octyl C-8 7 µm, 300 Å 07110055 07110056 07110057
Pecosphere C18 C-18, Monofunctional, Non-reduced, 3µm, 80A 02580164 0258-0220
(Pack of 5)
Pecosphere 3CR C-8, Base Deactivated, 3 µm (Pack of 5) 02580191
Pecosphere 3CR C-18, Base Deactivated, 3 µm (Pack of 5) 02580195
Pecosphere C18 100 x 3.2 mm monofunctional, non-reduced, 3 µm, 80A column 0258-0221
*Requires Holder (07150013) **Requires Holder (07150014)

33 x 4.6 mm i.d. 83 x 4.6 mm i.d. 150 x 4.6 mm i.d.

Normal Phase 5 per package 1 per package 1 per package
(requires holder 07150028) (requires holder 07150029) (requires holder 07150030)
Description Part No. Part No. Part No.
Pecosphere 3 Silica 02580167

Reversed Phase 33 x 4.6 mm i.d. 83 x 4.6 mm i.d. 150 x 4.6 mm i.d.

5 per package 1 per package 1 per package
Monofunctional; Pore Diameter 80 Å (requires holder 07150028) (requires holder 07150029) (requires holder 07150030)
Description Part No. Part No. Part No.
Pecosphere 3 C18, 3 µm 02580164 02580166
Pecosphere 5 C18, 5 µm 02580169
Pecosphere 3CR C8, Base Deactivated, 3 µm 02580191 02580192
Pecosphere 3CR C18, Base Deactivated, 3 µm 02580195 02580194
Reduced Activity 5 C18, 5 µm N9303396
Reduced Activity 5 C8, 5 µm 02580168
Peco HCODS C18, Polyfunctional, 5 µm, 300 Å 02580213
100 x 2.1 mm i.d. 100 x 3.2 mm i.d.
1 per package 1 per package
(requires holder 02580225) (requires holder 02580225)
Description Part No. Part No.
Pecosphere 3CR C18, Base Deactivated, 3 µm (Pack of 5) 02580220 02580221

30 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 BROWNLEE CARTRIDGE COLUMNS

33 mm x 4.6 mm i.d.4
Scavenger Columns 5 per package
Column Description Part No.
Silica Scavenger 10 µm 02580203
C18 Scavenger Monofunctional, 10 µm, 80A 02580202

100 mm x 2.1 mm i.d.2 220 mm x 4.6 mm i.d.5 220 mm x 2.1 mm i.d.5

Ion Exchange 1 per package 1 per package 1 per package
Column Description Part No. Part No. Part No.
AX-300, Aquapore 7 µm, 300 Å, Weak Anion 07110077
Polypore® CA 10 µm, Polymeric, Calcium Form 07110095
Polypore H 10 µm, Polymeric, Hydrogen Form 07110089

100 mm x 2.1 mm i.d.2 220 mm x 4.6 mm i.d.5 220 mm x 2.1 mm i.d.5

Polar Phase 1 per package 1 per package 1 per package
Column Description Part No. Part No. Part No.
Amino, Spheri-5 Aminopropyl, 5 µm, 80 Å 07110041
Cyano, Spheri-5 Cyanopropyl, 5 µm, 80 Å 07110047
Silica, Spheri-5 5 µm, 80 Å 07110035

100 mm x 2.1 mm i.d.2 220 mm x 4.6 mm i.d.5 220 mm x 2.1 mm i.d.5

Reversed Phase 1 per package 1 per package 1 per package
Column Description Part No. Part No. Part No.
BU-300, Aquapore Butyl C-4 7 µm, 300 Å 07110064
Cyano, Spheri-5 Cyanopropyl, 5 µm, 80 Å 07110047
OD-300, Aquapore ODS C-18, 7 µm, 300 Å 07110233 07110231 07110236
ODS, Spheri-5 C-18, Polyfunctional, 5 µm, 80 Å 07110022 07110023 07110024
Phenyl, Spheri-5 Phenyl, 5 µm, 80 Å 07110029
RP-8, Spheri-5 C-8, Monofunctional, 5 µm, 80 Å 07110004 07110005 07110006
Pecosphere 3CR C-18, Base Deactivated, 3 µm
RP-18, Spheri-5 C-18, Monofunctional, 5 µm, 80 Å 07110016 07110017 07110018
Pecosphere 3CR C-8, Base Deactivated, 3 µm
RP-300, Aquapore Octyl C-8 7 µm, 300 Å 07110058 07110059 07110060
Pecosphere C186 C-18, Monofunctional, Nonreduced, 3 µm, 80 Å 0258-0220
2
Requires Holder (07150014) 4
Requires Holder (07150028) 5
Requires Holder (07150015) 6
Requires Holder (02580225)

www.perkinelmer.com/supplies 31
 BROWNLEE CARTRIDGE COLUMNS

NewGuard cartridges directly coupled to a 220 mm MPLC

cartridge and connected externally to a 250 mm conventional column.

NewGuard Columns
NewGuard cartridges are small guard cartridges (15 mm x
3.2 mm i.d.) packed with 5 or 7 µm sorbents. NewGuard
cartridges prolong column life by eliminating particulates,
contaminant’s, and strongly bound sample components.
They act as replaceable, disposable heads of your analytical
columns. There is negligible loss of ef ciency and little effect
on retention or resolution. NewGuards are available in a
convenient 3-pack and can be coupled directly to any MPLC
cartridge with a union (07150018).

Features and Be
• Prolong column life by 2 to 5 times
• Protect your column from particulates and strongly bound
View of the NewGuard holder and a NewGuard 3-pack.
sample components
• Optimized dimensions to prevent loss of resolution
Standards Solutions
• Easy coupling to MPLC cartridges or conventional columns
• Finger-tight seal to 7,000 psi using NewGuard holders Description Part No.
• Can be used for sample preconcentration Test Mix
(connected to sample injection loop) LC Gradient Test Mix N9334010
Universal Test Mix for Reversed-phase (5 mL/pkg) 00890893
15 mm cartridge*, 3.2 mm i.d., 3 per pkg.
Standards
Description Size Shape Part No. HPLC SV Calibration Mix #5/610 PAH 00891542
Amino, Aquapore® Amino 7 µm Spherical 07110098 HPLC 610 Calibration Mix A 00891543
Anion, Aquapore Anion 7 µm Spherical 07110102 HPLC 610 Calibration Mix B 00891544
Cyano, Aquapore Cyano 7 µm Spherical 07110100
Diol, Aquapore Diol 7 µm Spherical 07110105
Phenyl, Aquapore Phenyl 7 µm Spherical 07110096
NewGuard Accessories
RP-2, Aquapore Dimethyl 7 µm Spherical 07110086 Replacement Seals for MPLC and Prep-10 Holders
RP-4, Aquapore Butyl 7 µm Spherical 07110088 Description Part No.
RP-8, Aquapore Octyl 7 µm Spherical 07110090 Prep-10 Inlet Seal
RP-18, Aquapore ODS 7 µm Spherical 07110092 One each 07150012
Silica, Aquapore Silica 7 µm Spherical 07110106 (Prep-10 Seal Replacement Kit Tools Required)

Validated®, C18 5 µm Spherical 00402237 Prep-10 Outlet Seal

One each 07150026
* Requires holder (07150001)
(Prep-10 Seal Replacement Kit Tools Required)
Note: Actual bed length of NewGuard is about 13 mm
Seals for MPLC Holders
Two 7,000 psi seals 07150024
(Seal Replacement Kit Tools Required)
Seal Replacement Kit for MPLC Holders
Tools and Two 7,000 psi Seals 07150023

32 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 BROWNLEE CARTRIDGE COLUMNS

Finger-Tight Holders
MPLC/Peco™ Holders
The patented MPLC cartridge system ensures leak-free operation up to 7000 psi, with only er-tightening. The high-pressure seal in
each end assembly actually seals tighter as pressure increases. No wrenches are required, and there is no need to disconnect any tubing
to your LC system when changing a cartridge. Each MPLC cartridge can be directly coupled to another using a union.

Name Description Part No.

Universal Holder Kit Includes 100 and 220 mm holder bodies, 2 end assemblies, 1 union and 1 NewGuard end assembly. 07150025

NewGuard® Holder Holds a single NewGuard cartridge or a single 20 mm CHOICE™ cartridge. 07150001

30 mm Holder Holds a single MPLC and CHOICE 30 mm cartridge. 07150013

(Do not use with Pecosphere column)

33 mm Pecosphere Holder Holds 33 mm Fast LC Peco cartridge only. 07150028

50 mm Holder Holds a single 50 mm CHOICE cartridge only. N2580030

83 mm Peco Holder Holds 83 mm Fast LC Peco cartridge only. 07150029

100 mm Holder Holds a single MPLC 100 mm cartridge. 07150014

100 mm Holder Holds Pecosphere 10 cm cartridge 02580225

150 mm Holder Holds a single MPLC 150 mm cartridge. 07150030

220 mm Holder Holds a single MPLC 220 mm cartridge. 07150015

250 mm Holder Holds a single MPLC 250 mm cartridge. 07150031

100 mm NewGuard System Holder Holder for a NewGuard and 07150016

a 100 mm coupled cartridge.

220 mm NewGuard System Holder Holder for a NewGuard and 07150017

a 220 mm coupled cartridge.

130 mm MPLC System Holder Holder for a 30 and 100 mm coupled cartridge. 07150032

Union For direct coupling of 2 cartridge holders. 07150018

(Two holder bodies and 2 end assemblies also required.)

NewGuard End Assembly Upgrades a 30 mm holder to a NewGuard holder. 07150002

End Assembly Holder component. 07150019

30 mm Holder Body Holder component. 07150020

100 mm Holder Body Holder component. 07150021

20 mm Holder Body Holder component. 07150022

Prep-10 Cartridge Holders

Name Description Part No.
100 mm Prep-10 Holder Holds a single 100 mm Prep-10 cartridge. 07150005

250 mm Prep-10 Holder Holds a single 250 mm Prep-10 cartridge. 07150006

www.perkinelmer.com/supplies 33
 SAMPLE LOOPS AND TUBING

Stainless Steel Sample Loops Sample loops are made from PEEK tubing and ttings. PEEK
(poly-ether-ether-ketone) is a mechanically-strong, chemically-inert
For Flexar FX UHPLC Autosampler polymer, ideal for HPLC applications where metal surfaces may
(up to 18,000 psi operation) interact with the mobile phase or sample component. Each
Stainless Steel loops required for use with FX-10 or FX-15 sample loop is supplied with two PEEK hex-head nuts and
systems operating above 6,200 psi. ferrules. These ttings grip the tubing in two locations for a
more reliable connection.
High Pressure Stainless Steel Sample Loops
Description Size Part No.
Description Size Part No.
PEEK Sample Loop 20 µL N9306035
Sample Loop, SS 2 µL N2936071
PEEK Sample Loop 50 µL N9306036
Sample Loop, SS 5 µL N2936056
PEEK Sample Loop 200 µL N9306038
Sample Loop, SS 10 µL N2936057
Internal Loop (PEEK and Ceramic) 2 µL N9306022
Sample Loop, SS 20 µL N2936058
Sample Loop, SS 50 µL N2936059
Tubing Accessories
Sample Loop, SS 100 µL N2936060
Sample Loop, SS 200 µL N2936061 Clean-Cut™ tubing tool is designed
Sample Loop, SS 500 µL N2936062
to cut Te ®
, Tefzel® and polymers in
general but, in particular, PEEK tubing. A unique safety locking
Sample Loop, SS 1 mL N2936063
mechanism secures the blade when not in use.
Sample Loop, SS 2 mL N2936064
Sample Loop, SS 5 mL N2936065 Description Part No.
Clean-Cut Tubing Cutter ED020015
Stainless Steel Sample Loops Clean-Cut Tubing Replacement Blade ED020016
Model 7125/7010 Model 7725 Model 8125 Cable/Tubing clip (U-Shaped) NX598006
Size Part No. Part No. Part No. Stick-on Tubing Organizer (Gray Plastic) NX598006
10 µL 09904938 N9306024
50 µL 09904940 N9306026 PEEK Tubing
100 µL 09904942 N9306027 41000013
PEEK tubing has the strength required to withstand continuous
200 µL 09904818 N9306028 09904818
use at HPLC pressure without swelling or bursting.
5 mL N9306032
Use PEEK with virtually any organic or inorganic liquid. PEEK
tubing is not affected by halide salts, high-strength buffers or
PEEK Sample Loops For Series 200/225 and other aggressive mobile phases that degrade stainless steel.
Flexar LC Autosamplers (up to 6,200 psi operation)
Description Length Part No.
These loops are compatible with all standard Flexar autosamplers 1
⁄16 in O.D. x 0.007 in I.D. 5 ft N9302678
as well as Series 225 autosamplers. PEEK loops cannot be used 1
⁄16 in O.D. x 0.010 in I.D. 5 ft N9302650
above 6,200 psi operation therefore are NOT compatible eith 1
⁄16 in O.D. x 0.004 in I.D. (Black) 50 cm N2916200
standard Flexar autosamplers. Stainless steel loops required for use 1
⁄16 in O.D. x 0.005 in I.D. (Red) 50 cm N2916059
wirh FX-10 or FX-15 UHPLC systems operating above 6,200 psi.
Connector Tubing, Flexar/FX Mixer to N2916211
Description Size Part No. Injector, SS

PEEK Sample Loop 2 µL N2936072 Connector Tubing, Flexar ISO/Binary/ N2916210

Quaternary Pump to Autosampler, SS
PEEK Sample Loop 5 µL N2936073
PEEK Sample Loop 10 µL N2936074
Stainless Steel Tubing
PEEK Sample Loop 20 µL N2936075
PEEK Sample Loop 50 µL N2936076 Features and Be
PEEK Sample Loop 100 µL N2936077 • Pre-cut and mirror-polished in 316 stainless steel
PEEK Sample Loop 250 µL N2936078
PEEK Sample Loop 500 µL N2936079 Description Length Part No.
PEEK Sample Loop 1 mL N2936080 1
⁄16 in O.D. x 0.015 in I.D. 6 ft 00873036
PEEK Sample Loop 2 mL N2936081 1
⁄16 in O.D. x 0.007 in I.D. 3 ft 02540838
PEEK Sample Loop 5 mL N2936082 1
⁄16 in O.D. x 0.005 I.D. 2 ft 02507060

34 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 FINGERTIGHT FITTINGS

ONE-PIECE,
High-performance Fingertight® ttings
for the most demanding applications
This PEEK Fingertight® tting is the toughest with regard to chemical

EASY-TO-USE
resistance and pressure. This high-performance Fingertight® tting is
recommended for the most demanding applications and will resist
pressures up to 6,000 psi (400 bar). Made from a single piece of
PEEK, the size permits tightening without tools.

Description Part No.

PEEK One-Piece Fingertight® Fitting ED020005

Fittings, Nuts and Ferrules

Stainless steel nuts are available in both Parker
Rheodyne™ formats. The nuts are used to connect 1⁄16 inch
Fittings Kits
o.d. stainless steel tubing and feature a 10-32 thread size.
PerkinElmer also of 1
⁄16 inch o.d. tubing Recommended for users of a PerkinElmer Biocompatible LC
in ⁄4-28 thread size.
1 system. Contains nuts, ferrules and unions.

Stainless Steel Fittings Description Part No.

Description Part No. Biocompatible Column Fittings for LC N9301001

Kel-F Reverse Ferrule, ⁄8 inch*

1
09903771
Fittings Kit for LC
Kel-F Ferrule, 1⁄16 inch* 09920382
Tefzel Nut, 1⁄16 inch* 09920381 The kit contains Rheodyne™ and SSI nuts and ferrules as well as
Parker errule, 1⁄16 inch 00873032 Fingertight® II nuts and ferrules for toolless installation. Zero-dead-
Parker ⁄16 inch
1
09903980 volume unions are also included. In addition you receive varying
lengths of stainless steel tubing in 0.007 and 0.010 inch i.d.s, as
Parker errule Kit, ⁄16 inch1
00890945
Includes: 6 nuts and 6 ferrules well as 0.30 inch i.d Tefzel® tubing.
Parker olume Union 09903289 Description Part No.
with nuts and ferrules
All-in-one Fittings Kit N9301002
Parker N2916202
Rheodyne™ Ferrule, 1⁄16 inch 09904947
Operation Kit
Rheodyne Nut, ⁄16 inch long body
™ 1
09904974
Rheodyne™ Nut, 1⁄16 inch short body 09904956 Recommended for purchasers of their rst LC. Includes tubing,
Rheodyne Nut and Ferrule Kit, ⁄16 inch
™ 1
02540274
union, ttings, syringe, basic LC book and test mix.
Includes: 6 ferrules, 3 short nuts and 3 long nuts
Description Part No.
Tefzel Nut (for Reverse Ferrule), 1⁄8 inch* N2601189
Operation Kit 00890873
Tefzel Nut, 1⁄16 inch* 09920381
Valco Ferrule 09903891
OptiTech Reusable Nut/Ferrule for UHPLC N9306301

OptiTechFerrule replacements for UHPLC (10 pack) N9306300

UHPLC ⁄16 inch Reusable Fitting
1
N9307800

* The Tefzel and K

www.perkinelmer.com/supplies 35
 VACUUM DEGASSING KITS

ENHANCE PUMP PERFORMANCE

Vacuum Degassing Kits Check Valves

Using a solvent degassing system will extend HPLC check valves allow solvent to w in
the performance of your pump. PerkinElmer only the desired direction. Check valves are
offers both vacuum degassing systems that easy to install with the torque wrench kit. For all
can handle all your degassing requirements. PerkinElmer pumps an intermediate check valve
is required. The input check valve, which is identical, should be
On-Line Vacuum Degasser Kit
ordered. The check valves of the biocompatible pumps differ from
This is a low-volume, high ef ciency, on-line module for the those of the stainless steel pumps in that all portions that contact
removal of dissolved gasses from HPLC solvents. The vacuum id are composed of titanium.
degasser is available in 3 and 5 channel models to support Inlet/Intermediate Outlet
isocratic, binary and quaternary pumps as well as degassing of Check Valve Check Valve
autosampler sh solvent. Type Part No. Part No.
Standard Stainless Steel 02540177 02540197
Description Part No.
Micropump 02540177 02540197
3-channel Vacuum Degassing Package. N2600571
Includes: a Vacuum Degasser, one 1 L Bottle with Biocompatible Titanium N2600226 N2600192
Cap, one 2 L Bottle with Cap, one Organizer Tray and
FX-15 Check Valves N2911220 N2911220
Accessory
5-channel Vacuum Degassing Package. N2600570
Includes: a Vacuum Degasser, two 1 L Bottle Maintenance Kits and Tools
with Caps, two 2 L Bottles with Caps, Solvent Tray
and Organizer Description Part No.
Binary Bottle Cap Kit. N2600522 Biocompatible Piston Seal Replacement Kit N2910385
Includes: two Caps, Tubing, Fittings and Labels Includes: Four Seals, Backup Rings, and O-rings
required for two Solvent Bottles
Check Valve Torque Wrench 02540871
Quatenary Bottle Cap Kit. N2600523
Diaphragm for Pulse Compensator N2601316
Includes: four Caps, Tubing, Fittings and Labels
required for four Solvent Bottles FX-15 UHPLC Pump Accessories N2911224
Replacement 0.5 µm Filter Frits for FX-15 (4-pack)

Liquid Chromatography Laboratory Bottle Kits High pressure FX-15 Piston Seal Kit N2911221
Standard Piston Seal Replacement Kit N2910383
Contents Part No. for Series 200 and Flexar Pump
1x5 Liter Bottle with Cap and T 2 Meters N2601610 Includes: Four Seals, Backup Rings, and O-rings
PTFE 1⁄8" Tubing and 1x10 µm SS Solvent Frit Insertion Tool N2601503
1x2 Liter Bottle with Cap and T N2601611 Micropump Piston Seal Replacement Kit N2910384
PTFE 1⁄8" Tubing and 1x10 µm SS Solvent Frit for Series 275 and FX-10 pump
1x1 Liter Bottle with Cap and T N2601612 Piston Wash Bottle Kit for FX-15 N2601616
PTFE 1⁄8" Tubing and 1x10 µm SS Solvent Frit
Pulse Compensator Repair Kit N2600313
1x0.5 Liter Bottle with Cap and T N2601613 Includes: Diaphragm, Elastomer Plug, and Seal
PTFE 1⁄16" Tubing and 1x40 µm SS Solvent Frit
Seal Removal Tool N2601295
1 Cap with T 1
⁄8" Tubing and N2601614
1x10 µm SS Solvent Frit Series 200 Bio LC Pump Maintenance Kit N2910346
Includes: Fuses, Seals, O-rings, and Seal Tools
1 Cap with T 1
⁄16" Tubing and N2601615
1x40 µm SS Solvent Frit Series 200 Pump Maintenance Kit N2910345
Includes: Fuses, Seals, O-rings, and Seal Tools
1x0.5 Liter Bottle with Cap and Dual T N2601616
Insert 2 pcs of 2 Meter PTFE 1⁄8" Tubing (Piston
Wash Function) Replacement Vacuum Degasser Bottles
1 Cap with Dual T N2601617
Description Part No.
PTFE 1⁄8" Tubing and 1x10 µm SS Solvent Frit (Piston
Wash Function) 1 L Glass Bottle N2600497
2 L Glass Bottle N2600498

36 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 SOLVENT FILTRATION AND MIXERS

Solvent Sparger and Solvent Filters

Solvent lters remove unwanted
UHPLC Static Mixers (up to 18,000 psi)
particulate matter from the LC
instrument. Spargers connect Description Size Part No.
directly to the solvent delivery line In-Line High Pressure Mixer Assembly, SS N2911200
and are easily removed for cleaning. In-Line High Pressure Mixer Assembly, SS 150 N2911201
The scavenger column is ideal for Solvent Filters In-Line High Pressure Mixer Assembly, SS 250 N2911202
eliminating particulate material from solvents.
In-Line High Pressure Mixer Assembly, SS 350 N2911205
Solvent Spargers, Filters and Scavengers In-Line High Pressure Mixer Assembly, SS 500 N2911203

Description Size Part No. Binary T-Mixer, SS N2911170

Solvent Reservoir Sparger Binary High Pressure T-Mixer, SS 50 uL N2911212

Stainless Steel 10 µm 09903610 HP Binary High Pressure T-Mixing, SS 150 N2911206

Titanium 10 µm N2600070 Binary High Pressure T-Mixer, SS 350 uL N2911210

Stainless Steel 40 µm 09903615 Binary High Pressure T-Mixer, SS 500 uL N2911211

Titanium 40 µm N2600089 T-connector, SS N2911127

Solvent Filters/Scavengers
Analytical Static Mixers (up to 6,200 psi)
In-Line Solvent Filter System 09903606
In-Line Solvent Filter Replacement Kit 02540311 Description Size Part No.
In-Line Solvent Filter System, Titanium N2600259 Binary T Mixer 10 µL N2911170
Replacement 2 µm Titanium Filter Element N2601477 Binary T Mixer 25 µL N2911171
Replacement Seal for In-Line Solvent Filter N2601262 In-Line Mixer Assembly 150 µL N2911173
3 cm Scavenger Cartridge Holder 02580178 In-Line Mixer Assembly 500 µL N2911172
02580202 Binary T Mixer Cartridge 10 µL N2911174
LC System Accessories Binary T Mixer Cartridge 25 µL N2911175
Solvent waste cup N2916019 Binary T Mixer Cartridge 50 µL N2911176
Drain Cup N2916085 Binary T Mixer Cartridge 150 µL N2911177
90° Elbow Adapter, ⁄4" ID HDPP
1
09220102 Binary T Mixer Cartridge 250 µL N2911178
Tubing Clip (U-shaped, Polypropylene) NX598006
Polypro Elbow Barbs 09220102 Dynamic Mixer
Large ID (11 mm) Silicone Tubing (5 ft) N2916016 Used to facilitate complete mobile phase blending, resulting in
Manual Injector Bracket (Magnetic Clip-on) N2931275 improved retention performance. The mixer will most typically be
used as part of a Micro pump package — where high-pressure
blending of 2 pump outputs is required. Three different volume
mixing heads (75, 200 and 400 µL) are available.
Static Mixers
Kit includes: Mixer Accessory, Stainless Steel “Tee” Fitting (N2911127), U.S.
Static mixers are used to facilitate complete mobile phase style AC Line Cord and 0.1 A Type T Fuse.
blending, resulting in improved retention performance. The mixer
Description Part No.
will most typically be used where high-pressure blending of two
Dynamic Mixer Accessory (100–240 V) N2910520
pump outputs is required. Mixers incorporate a highly ef cient
cross w shearing mechanism which produces vortex sheer
Mixing Heads
mixing over a wide w range.
Size Part No.
75 µL N2910521
200 µL N2910522
400 µL N2910523
Mixers
Mixing Head

www.perkinelmer.com/supplies 37
 INJECTION VALVES AND ROTOR SEALS

NEW

Flom Analytical Injection Valve Rheodyne™ 9725 PEEK

The Flom Analytical Injection Valve is a very reliable manual HPLC Bio Injection Valve
injection valve. It comes with a built in “Inject” contact closure Model 9725 is inert and well-suited to the chromatography of
connection output. A PEEK rotor seal is provided for excellent biological molecules, including applications with aggressive mobile
solvent/sample r phases. This valve is useful in all applications in which metal
minimal dispersion. This valve is available in either stainless steel contact with the mobile phase and or sample should be avoided.
This valve uses PEEK vent lines and a T ®
rotor seal and can be
operated in a pH range from 0 – 14.
Description Product No.
Flom Injection Valve Stainless Steel N9306073 Description Product No.
Flom Injection Valve PEEK N9306072 9725 Injection Valve for Series 200 Autosampler N9306020

Injector Valve Rotor Seals Rheodyne™ 8125 Low-Dispersion

For both Series 200 Autosampler and manual injectors. Stainless Steel Injection Valve

For both the Rheodyne and Flom valves, rotor seals should be Ideal for use in an LC/MS system, the model 8125 is designed for
periodically replaced every 6 months to a year and are available 1 and 2 mm microbore columns and can also be used with
in PEEK. For the Rheodyne valve, they are also available in Vespel.
PEEK is more inert towards amines and other basic biomolecules/ produce low dispersion, maintaining the high mass sensitivity
pharmaceuticals, exhibiting less sample carryover. However, Vespel inherent in micro columns.
is somewhat more resilient than PEEK, providing somewhat better Description Product No.
wear life. 8125 Low-Dispersion Stainless Steel Injection Valve N9306021

Description Product No.

Flom Rotor Seal PEEK N9306068
Rheodyne ™ 7725 Analytical Injection Valve
Rheodyne 7725 Vespel Rotor Seal 09904802 Inject from 1 µL to 5 mL with high accuracy and precision,
Rheodyne 8125 Vespel Rotor Seal 10070900 with the Rheodyne™ 7725 and 7725i valves.
Rheodyne 7725/9725 PEEK Rotor Seal N9306044 Rheodyne™ 7725 and 7725i valves are versatile injectors and

loading the sample loop. Wide port angles of the 7725/7725i

valve provide impr
2 µL internal capacity is also available. In a clean system, the
7725/7725i typically can make more than 30,000 injections before
requiring replacement of the rotor seal.

Description Product No.

7725 Injection Valve for Series 200 Autosampler N9306019
7725i Injection Valve with Internal Switch N9306017

38 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 FLOW CELLS AND PUMP ACCESSORY KITS

INTERCHANGEABLE
FOR YOUR SAMPLE
Bio Conversion Kits Flexar UV/Vis, Series 200/785A UV/Vis,
There are two kits available; one that contains all of the parts Flexar PDA and Series 200 EP
necessary to convert the Series 200 pump and Series 200
These wcells provide the highest detection limits possible for
autosampler for Bio applications. The other contains all of
your sample. The 6 mm pathlength wcell has been optimized
the parts necessary to convert only the Series 200 Pump
for lower-dispersion LC analysis and is the perfect choice when
for Bio applications.
using narrowbore, 2.1 mm or microbore, 1 mm columns (see
Description Part No. Figure 1). The 3 mm pathlength is the choice for semiprep LC to
S200 Pump and S200 Autosampler Conversion Kit N2910035 avoid detector saturation at high-solute concentrations.
S200 Pump Conversion Kit N2910036
UV/Vis, PDA, FL and LC-135/235 FLOWCELLS

Pump Seal Kits Description Size Part No.

Flowcells for Flexar FX-UV/Vis UHPLC Detectors
Description Part No.
Flowcell 6 mm x 2.4 µL N2925090
High Pressure Piston Seal Replacement Kit N2910383
Includes: Seals (4), Backup Rings and O-Rings 6 mm x 2.4 µL N2920070
detector head plate w/ gasket)
Micropump Piston Seal Replacement Kit N2910384
Includes: Seals and O-Rings Flowcells for Flexar FX-UV/Vis UHPLC Detectors

Series 200 Pump Maintenance Kit N2910345 10 mm x 15 µL N2920070

Includes: Fuses, Seals, O-Rings and Seal Tools w/ heat exchanger and zdv unions)
Flowcell 10 mm x 15 µL N2920124
Flowcell Assembly (w/ zdv unions) 6 mm x 2.4 µL 29000544
Prep Flowcell Assembly 3 mm x 1.7 µL 29000545
(w/ zdv unions)
Flowcells for 785A UV/Vis Detectors
Flowcell 8 mm x 12 µL 29000542
Flowcell Assembly 8 mm x 12 µL N2920117
(w/ heat exchanger and zdv unions)
Flowcells for Flexar FX-PDA UHPLC and Series 275 PDA Detectors
Detector Backpressure Regulator Flowcell (dual-lensed) 6 mm x 2.4 µL N2920128

The detector backpressure regulator is a device that is attached to Flowcells for Flexar and Series 200EP PDA Detectors

the outlet of the detector to prevent outgassing in the wcell, Flowcell 10 mm x 15 µL N2920126
eliminating variations to the detector baseline. Flowcell Assembly 10 mm x 15 µL N2920160
Flowcells for Series 200 PDA Detectors
Description Material Part No.
Flowcell 10 mm x 12 µL N2922107
40 psi inline backpressure regulator N2925090
Flowcell 4.5 mm x 5 µL N2922030
2 – 5K psi variable N2925091
backpressure regulator FL 3 µL Flowcell w/ Assembly N2922211
Backpressure Regulator Stainless 09907126 Flowcells for LC-135/235 PDA Detectors
Adjustable from 15 – 59 psi Steel Flowcell Assembly 10 mm x 8 µL N2350211
(w/ heat exchanger)
Flowcell 10 mm x 8 µL N2350162

www.perkinelmer.com/supplies 39
 HPLC AND UHPLC DETECTOR LAMPS

FOR OUTSTANDING
PERFORMANCE
Series 200/200 EP
Photo Diode Array Detector Lamp

Photo Diode Array Detector Lamps

The Flexar Series/Series 200/200 EP Photo Diode Array Detector
provides true UV/VIS detection and high resolution spectral data.
The excellent signal-to-noise characteristics make it ideally suited
UV/VIS Deuterium
Detector Lamp for low-volume or low concentration samples.

Components Part No.

Flexar/Series 200 EP
Deuterium Lamp N2925030
Our extensive quality control and inspection process demands
the very best quality sources. Choosing a PerkinElmer deuterium, Tungsten Lamp N2922011

tungsten or xenon source provides outstanding ultraviolet and Series 200

true visible performance. Deuterium Lamp (Phase 2) N2922046

Tungsten Lamp N2922011
LC-135C/235
• Exceptional performance anywhere in the detector’s 190 – 700 nm
Detector Lamp N2351285
wavelength range
• Lamp changes are quick and easy due to a unique self-aligning lamp
mount Fluorescence Detector Lamps
The major bene t afforded by rescence detection is
UV/VIS Detector Lamps the inherent high sensitivity of the technique coupled with
outstanding speci city. The Series 200a Fluorescence Detector
Components Part No.
provides signal to noise ratio of >700:1 from trace analysis
Flexar Series/Series 200/785A UV/VIS Detector Lamps
using a 150 W xenon source.
Deuterium Lamp N2920149
Components Part No.
Tungsten Lamp N2920146
Flexar/Series 200/200a
LC-295 UV/VIS Detector Lamps
Xenon Lamp N2922082
Deuterium Lamp 02712266
Flexar/Series 200/200a

Refractive Index Detector Lamps Xenon Lamp L2251157

Festoon Lamp 04969486
The Flexar Series/Series 200/200s Refractive Index, with its
de ection-type design, allows sensitive detection of these
compounds with low noise and drift characteristics.
Series 200/200a
Components Part No.
Tungsten Lamps 02712273

40 CHROMATOGRAPHY: LIQUID CHROMATOGRAPHY

 NITROGEN GENERATORS

Nitrogen Generators Description Part No.

N2-22 Mid Flow Nitrogen Generator N9306210
PerkinElmer offers customers a new line of nitrogen
without Air Compressor
generators to meet the growing need for cost
effective and pure nitrogen. Product Specification
Purity: 95.0 – 99.5%
Membrane Nitrogen Generators are designed to
Atmospheric Dewpoint -58 °F (-50 °C)
supply single or multiple LC/MS instruments with dry
Particles >0.01 μm: None
nitrogen at purities of 98% to 99.5%.
Suspended liquids: None
The generator can also be used for solvent Min./Max. operating pressure: 60/145 psig (4.0/10 barg)
evaporation as well as supplying dry nitrogen to Ambient operating temperature 60 °F – 95 °F (15 °C – 35 °C)
Flow Rates*
analytical instruments. range:
Inlet air temperature range: 60 °F – 95 °F (15 °C – 35 °C) Inlet Output Purity
psi Ipm %
Features and Be Maximum recommended inlet air 110 °F (43 °C)
100 13 99.5
temperature
• LC/MS grade purity enhances instrument 125 16 99.5
Electrical requirements: None
performance 100 20 99.0
Dimensions (net): 45 cm W x 41 cm D x 130 cm H
• Phthalate-free 125 25 99.0
Weight (shipping): 36 kg, 80 lbs 100 30 98.0
• Recommended, certi d, tested, and used by all major
LC/MS instrument manufacturers Description Part No. 125 38 98.0

• Return on investment in 6 months to 2 years N2-35 Mid Flow Nitrogen Generator N9306211
without Air Compressor
• Minimal maintenance required
Product Specification
Applications Purity: 95.0 – 99.5%
• LC/MS Atmospheric Dewpoint -58 °F (-50 °C)
• Solvent Evaporation Particles >0.01 μm: None
• Analytical instruments requiring nitrogen Suspended liquids: None

• rmal Analyzers Min./Max. operating pressure: 60/145 psig (4.0/10 barg)

Ambient operating temperature 60 °F – 95 °F (15 °C – 35 °C)
range:
Low Flow NITROFLOW Lab Inlet air temperature range: 60 °F – 95 °F (15 °C – 35 °C) Flow Rates*
Maximum recommended inlet air 110 °F (43 °C) Inlet Output Purity
Features and Be temperature psi Ipm %
• Complete “Plug and Play” system recommended by Electrical requirements: None 100 20 99.5
all major LC/MS manufacturers Dimensions (net): 45 cm W x 41 cm D x 130 cm H 125 25 99.5
100 30 99.0
• 8,000 hours compressor warranty or two years Weight (shipping): 41 kg, 90 lbs
125 37 99.0
• Special design ensures quiet operation Description Part No. 100 46 98.0
• Dual oil-less air compressors N2-45 High Flow Nitrogen Generator N9306212 125 57 98.0
without Air Compressor
Components Part No. Product Specification
120 V N9306213 Purity: 95.0 – 99.5%
230 V N9306214 Hydrocarbon-free
Phthalate-free
Product Specification
Purity: 94.0 – 99.5% Atmospheric Dewpoint -58 °F (-50 °C)
Phthalate-free Particles >0.01 μm: None
Hydrocarbon-free Suspended liquids: None
Flow Rates 10 – 50 lpm Min./Max. operating pressure: 60/145 psig (4.0/10 barg)
Delivery pressure 8 bar Recommended ambient 72 °F (22 °C)
Ambient temperature 10 °C – 35 °C operating temp range
Flow Rates*
Power consumption 1400 Watts Inlet air temperature range: 60 °F – 95 °F (15 °C – 35 °C)
Inlet Output Purity
Noise level <58 dB (A) Maximum recommended inlet 110 °F (43 °C) psi Ipm %
air temperature
Electrical requirements 120 V / 60 Hz, 20 Amp Nema 5 100 39 99.5
Lab capacity at nominal conditions: Inlet/Outlet ports 1/2” NPT
125 55 99.5
Ambient temperature: 20 °C/ 68 °F Electrical requirements: None ation system only) 100 53 99.0
Ambient pressure: 1013 mbar(a). Dimensions (net): 61 cm W x 50 cm D x140 cm H 125 74 99.0
Dimensions (net): 90 cm W x 31 cm D x 70 cm H Weight (shipping) 114 kg, 250 lbs 100 73 98.0
Weight (shipping) 127.4 kg, 280 lbs *See www.perkinelmer.com for complete listing of rates. 125 106 98.0

www.perkinelmer.com/supplies 41