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Dr. The abstract should be a single paragraph of 150-250 words written in the past tense. It should state the (1) objectives of the study, (2) methodology, (3) results, and (4) conclusions. Do not cite any references or use footnotes. This study aimed to determine the optimal conditions for the production of yellow pigments by Monascus purpureus through response surface methodology. A 2k factorial design was used to evaluate the effects of temperature, initial pH, and incubation time on pigment production. Monascus purpureus was cultivated in potato dextrose broth under different combinations of the independent variables. Pigment concentration was measured spectroph

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0% found this document useful (0 votes)

424 views

Ceat Format 2016

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James Matthew Limpin

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THESIS TITLE IN UPPER CASE AND BOLD LETTERS

IN INVERTED PYRAMID FORMATTING

TITLE PAGE

AUTHOR’S FULL NAME IN ALL CAPS

SUBMITTED TO THE FACULTY OF

DEPARTMENT OF CHEMICAL ENGINEERING
COLLEGE OF ENGINEERING AND AGRO-INDUSTRIAL TECHNOLOGY
UNIVERSITY OF THE PHILIPPINES LOS BAÑOS
IN PARTIAL FULFILLMENT OF THE
REQUIREMENTS FOR THE
DEGREE OF

BACHELOR OF SCIENCE IN CHEMICAL ENGINEERING

JUNE 2017

This thesis manuscript can be accessed by:

By the general public -

Only after the consultation with author or adviser -
Only by those bound by confidentiality agreement Yes

Signature of Student:
Signature of Thesis Adviser:

i
The thesis attached hereto, entitled “INSERT THESIS TITLE IN ALL CAPS”

prepared and submitted by INSERT FIRST MIDDLE FAMILY NAME IN ALL CAPS

in partial fulfillment of the requirement for the degree of BACHELOR OF SCIENCE IN

CHEMICAL ENGINEERING, is hereby accepted.

APPROVAL PAGE

PANEL MEMBER ALL CAPS PANEL MEMBER ALL CAPS

Member Member
Guidance Committee Guidance Committee

Date Signed Date Signed

ADVISER ALL CAPS CO ADVISER ALL CAPS

Adviser and Chair Co-Adviser
Guidance Committee Guidance Committee

Date Signed Date Signed

MANOLITO E. BAMBASE, JR
Chair
Department of Chemical Engineering

Date Signed

ARNOLD R. ELEPAÑO
Dean
College of Engineering and Agro-Industrial Technology

Date Signed

ii
BIOGRAPHICAL SKETCH

Insert photograph (colored studio photo wearing decent formal attire with white

background 4.5 cm x 3.5 cm text-wrapped at the upper right portion of the first paragraph

of the biographical sketch.

iii
ACKNOWLEDGMENT

iv
TABLE OF CONTENTS

PAGE

TITLE PAGE i

APPROVAL PAGE ii

BIOGRAPHICAL SKETCH iii

ACKNOWLEDGMENT iv

TABLE OF CONTENTS v

LIST OF TABLES viii

LIST OF FIGURES ix

LIST OF APPENDICES x

LIST OF APPENDIX TABLES xiv

LIST OF APPENDIX FIGURES xv

ABSTRACT xvi

1. INTRODUCTION 1

1.1 Background of the Study 1

1.2 Significance of the Study 1

1.3 Objectives of the Study 1

1.4 Scope and Limitations of the Study 1

1.5 Time and Place of the Study 1

2. REVIEW OF LITERATURE 3

2.1 Type Subheading Title. DO NOT DELETE NUMBER 3

2.2 Type Subheading Title. DO NOT DELETE NUMBER 3

v
2.3 Type Subheading Title. DO NOT DELETE NUMBER 3

2.4 Type Subheading Title. DO NOT DELETE NUMBER 3

2.5 Type Subheading Title. DO NOT DELETE NUMBER 3

2.6 Type Subheading Title. DO NOT DELETE NUMBER 4

2.7 Type Subheading Title. DO NOT DELETE NUMBER 4

2.8 Type Subheading Title. DO NOT DELETE NUMBER 4

2.9 Type Subheading Title. DO NOT DELETE NUMBER 4

3. MATERIALS AND METHODS 6

3.1 Type Subheading Title. DO NOT DELETE NUMBER 6

3.2 Type Subheading Title. DO NOT DELETE NUMBER 6

3.3 Type Subheading Title. DO NOT DELETE NUMBER 6

3.4 Type Subheading Title. DO NOT DELETE NUMBER 6

3.5 Type Subheading Title. DO NOT DELETE NUMBER 6

3.6 Type Subheading Title. DO NOT DELETE NUMBER 7

3.7 Type Subheading Title. DO NOT DELETE NUMBER 7

3.8 Type Subheading Title. DO NOT DELETE NUMBER 7

4. RESULTS AND DISCUSSION 8

4.1 Type Subheading Title. DO NOT DELETE NUMBER 8

4.2 Type Subheading Title. DO NOT DELETE NUMBER 8

4.3 Type Subheading Title. DO NOT DELETE NUMBER 8

4.4 Type Subheading Title. DO NOT DELETE NUMBER 8

4.5 Results of Response Surface Methodology Experiments 8

4.6 Type Subheading Title. DO NOT DELETE NUMBER 9

4.7 Type Subheading Title. DO NOT DELETE NUMBER 9

4.8 Type Subheading Title. DO NOT DELETE NUMBER 9

vi
4.9 Type Subheading Title. DO NOT DELETE NUMBER 9

4.10 Type Subheading Title. DO NOT DELETE NUMBER 10

5. SUMMARY AND CONCLUSION 11

6. RECOMMENDATIONS 12

7. REFERENCES 14

APPENDICES 15

APPENDIX A 16

APPENDIX B 17

APPENDIX C 18

APPENDIX D 19

APPENDIX TABLES 20

APPENDIX FIGURES 22

vii
LIST OF TABLES

TABLE PAGE

3-1 Factors considered in the preliminary experiments for yellow 22

pigment production

3-2

viii
LIST OF FIGURES

FIGURE PAGE

2-1 Chemical structures of the Monascus pigments 10

3-1

ix
LIST OF APPENDICES

APPENDIX PAGE

A Determination of Biomass Concentration 55

B Determination of Residual Glucose Concentration 56

C Determination of Orange and Yellow Pigment 58

Concentration

D Calculation for the Amount of MSG from C:N Ratio 59

x
xi
LIST OF APPENDIX TABLES

APPENDIX PAGE
TABLE

1 Data of absorbance against glucose concentration at 550 nm 62

xii
LIST OF APPENDIX FIGURES

APPENDIX PAGE
FIGURE

1 Standard calibration curve of absorbance against glucose 65

concentration read at 550 nm

13 Plot of residual versus predicted for residual glucose concentration 71

in the factorial flask experiment

14 Plot of residuals versus run for residual glucose concentration 71

in the factorial flask experiment

15 Plot of predicted versus actual for residual glucose concentration 72

in the factorial flask experiment

16 Box-Cox plot for residual glucose concentration in the 72

factorial flask experiment

xiii
LIST OF APPENDIX TABLES

APPENDIX PAGE
TABLE

1 Data of absorbance against glucose concentration at 550 nm 62

xiv
LIST OF APPENDIX FIGURES

APPENDIX PAGE
FIGURE

1 Standard calibration curve of absorbance against glucose 65

concentration read at 550 nm

13 Plot of residual versus predicted for residual glucose concentration 71

in the factorial flask experiment

14 Plot of residuals versus run for residual glucose concentration 71

in the factorial flask experiment

15 Plot of predicted versus actual for residual glucose concentration 72

in the factorial flask experiment

16 Box-Cox plot for residual glucose concentration in the 72

factorial flask experiment

xv
ABSTRACT

FAMILY NAME FOLLOWED BY COMMA, THEN FIRST AND MIDDLE NAME

ALL CAPS. College of Engineering and Agro-Industrial Technology, University of the
Philippines Los Banos. May 2017. Insert Thesis Title with Only the First Letter of Each
Word Capitalized.

Major Adviser: Dr. Beloved M. Adviser

Co-Adviser: Dr. Kind M. CoAdviser

The effects of the two blah blah, … fermentation performance of 30-L bioreactor
was concluded to be reproducible compared to 5-L bioreactor and the use of the kLa
correlation was found to be useful as a scale-up factor.

xvi
1. INTRODUCTION