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Tutorial Worksheet #2 Bioenergetics 2019-20

1) This document provides 10 tutorial questions about bioenergetics and thermodynamics for a semester 1 biochemistry course in 2019-2020. The questions cover topics like applying classical thermodynamics to open biological systems, the second law of thermodynamics and protein folding, what Gibbs free energy tells us about chemical reactions, determining redox potentials experimentally, and relating Gibbs free energy to membrane potential and ion concentration.

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317 views

Tutorial Worksheet #2 Bioenergetics 2019-20

1) This document provides 10 tutorial questions about bioenergetics and thermodynamics for a semester 1 biochemistry course in 2019-2020. The questions cover topics like applying classical thermodynamics to open biological systems, the second law of thermodynamics and protein folding, what Gibbs free energy tells us about chemical reactions, determining redox potentials experimentally, and relating Gibbs free energy to membrane potential and ion concentration.

Uploaded by

amy
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOC, PDF, TXT or read online on Scribd
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BIOC 2061

SEMESTER 1 2019-20
BIOENERGETICS TUTORIAL QUESTIONS

1) Describe the systems used in thermodynamics and explain how classical


thermodynamics can be applied to open systems such as biology?

2) What does the second law of thermodynamics state? If the entropy of a folded
protein is lower than that of the protein in its denatured state explain how the
folding process be spontaneous?

3) What information does a G value give about a chemical reaction and what
information does it not supply?

4) The reaction catalyzed by malate dehydrogenase has a  Go/ of +29kJmol-1. Can


this reaction proceed spontaneously? Explain your answer.

5) Explain the structural basis of the high group transfer potential of ATP.

6) Explain, with the use of a graph, why ATP is able to perform useful work in a
cell?

7) Explain how you would experimentally determine the Eo value of the


NAD+/NADH redox couple.

8) Split the flowing reaction into its two half reactions

Succinate + FAD ↔ fumerate + FADH2


9) Consider the reaction:

isocitrate + NAD+ ↔ -ketoglutarate + CO2 +NADH

Under the observed conditions the E value for the NAD+/NADH couple = -0.32 V
and the E value for the isocitrate/ -ketoglutarate couple = -0.38V

Would this reaction proceed spontaneously or not? Explain your answer.

10) Show how G is related to membrane potential and ion concentration?

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