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Lab 1 Experiment 9

This experiment measured dissolved oxygen levels in rice water and swimming pool water samples under different temperature conditions. The objectives were to investigate how temperature affects oxygen solubility. Samples were tested before and after aeration at chilled (4-8°C) and warmed (38°C) temperatures. Results found that oxygen levels decreased in chilled samples but increased after aeration. Warmed unaerated samples had higher oxygen than baseline but levels further increased with aeration. However, the results did not match the theoretical relationship between temperature and oxygen solubility. Limitations included ignoring other solubility factors.

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45 views

Lab 1 Experiment 9

This experiment measured dissolved oxygen levels in rice water and swimming pool water samples under different temperature conditions. The objectives were to investigate how temperature affects oxygen solubility. Samples were tested before and after aeration at chilled (4-8°C) and warmed (38°C) temperatures. Results found that oxygen levels decreased in chilled samples but increased after aeration. Warmed unaerated samples had higher oxygen than baseline but levels further increased with aeration. However, the results did not match the theoretical relationship between temperature and oxygen solubility. Limitations included ignoring other solubility factors.

Uploaded by

yuanbing0906
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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EXPERIMENT 9: INVESTIGATION OF OXYGEN SOLUBILITY IN WATER

OBJECTIVES

 To measure dissolved oxygen in water samples at different temperatures.


 To investigate oxygen solubility in different water samples.
 To learn the specific sampling technique in determining dissolved oxygen
concentration in water samples.

INTRODUCTION

Dissolved oxygen levels in environment water depend on the physiochemical and


biochemical activities in water body and it is an important useful in pollution and water
treatment process control. Without an appreciable level of DO, many kinds of aquatic
organisms cannot exist in water. A high DO level is needed for the aquatic organism to live
and it makes drinking water taste better than usual. High DO level is important to every living
organism. However, industries reduce to least possible amount of dissolved oxygen in order to
reserve it pipeline from corrode.

The level of DO in water is dependent on many physical, chemical, and biochemical


factors—aeration, wind, velocity of water flow, algae, temperature, atmospheric pressure,
organic compounds, salt content, bacteria, and animals. Temperature has a significant impact
on the solubility of oxygen in water. Increasing the temperature will normally decrease the DO
concentration in water. It is important to distinguish between oxygen solubility, which is the
maximum DO concentration at equilibrium, and the actual concentration of DO, which is
generally not the equilibrium concentration and is limited by the rate at which oxygen dissolves.
Water saturated with oxygen at 25oC contains 8.4 mg/L. The measurement of dissolved oxygen
is also important to determine whether a water system is predominantly aerobic or anaerobic,
predict the survival of aquatic organisms.
MATERIALS AND APPARATUS

Beakers, ice, water samples (rice water, swimming pool water), oxygen sensitive electrode,
thermometer, heater.

PROCEDURES

1. Two water samples were collected which are rice water and swimming pool water.
2. Rice water sample was divided equally into two separate beakers:
a. Beaker 1 was cooled with ice,
b. Beaker 2 was heated slightly to 35°c – 40°c.
3. The dissolved oxygen content of the water was tested first before the experiment
started. The measured oxygen content became the baseline measurement for dissolved
oxygen.
4. Ice was added to a larger beaker until enough, then some water was added and beaker
1 was immersed into it.
5. The water temperature was measured until it went around 4-8°c, the amount of
dissolved oxygen concentration was recorded.
6. Next, the water sample was aerated by pouring the water back and forth between two
beakers for 5-10 minutes.
7. The temperature of the water sample was checked periodically and more ice was
added to maintain the temperature.
8. When the water had been aerated, the dissolved oxygen test was repeated.
9. The similar test was run for swimming pool water sample.

RESULT

Condition Aerated DO Sample 1: Rice Water DO Sample 2: Swimming


(ppm) Pool Water (ppm)
Baseline - 1.0 2.5
Chilled - 0.4 2.2
Chilled  3.4 3.1
Warmed - 2.0 7.2
Warmed  7.0 7.8
Table 1: Oxygen Content Levels
DISCUSSION

Before the experiment starts, the baseline for each water samples was measured. The
oxygen baseline for rice water is 1.0ppm and 2.5ppm for swimming pool water. The presence
of microorganisms and biodegradation processes affect the dissolved oxygen level. This can
vertically, decreasing in the water column due to stratification.
Based on the observation of these two water samples, we can observe that the oxygen
content was decreased in chilled condition (low temperature) and it was lower than the
baseline. After aerated in chilled condition which is around 8°c, the oxygen content was
increased and higher than the baseline for both water sample which the rice water has oxygen
content of 3.4ppm while swimming pool water has oxygen content of 3.1 ppm.

Then, both water samples were heated to temperature of 38°c. The oxygen content
measured for rice water is 2.0 ppm while for swimming water is 7.2 ppm which is much
higher than the baseline. After aerated the warmed water samples, rice water has oxygen
content of 7.0 ppm and swimming pool water has oxygen content of 7.8 ppm. From the
readings measured, it is obvious to see that the oxygen solubility is higher after aerated in
heated condition at 38 °c.

Based on theory, a difference in DO levels may be seen between winter water


temperatures and summer water temperatures. Similarly, a difference in DO levels may be
apparent at different depths of the water if there is a significant change in water temperature.
The solubility of oxygen decreases as the temperature increases. In this experiment, we did
not manage to get the theoretical result. This may due to the error and limitation of this
experiment.

LIMITATION AND PRECAUTIONS

Firstly, only temperature and aeration were focus in this experiment which we ignore
about other factors such as the pressure and impurities in water sample that could affect the
oxygen content in water. Secondly, during sampling, no particular precautions are taken to
avoid contact between water and air, the water will take up oxygen and the figures will be too
high. Various means should be used to eliminate this error. Usually an excess o water is
allowed to flow through the bottle while for limnological investigation, various special types
of sampling apparatus have been designed.
CONCLUSION

The analysing method was not fully successfully applied for measuring oxygen solubility
in rice water and swimming pool water. In this experiment, the solubility of oxygen increased
in high temperature and aerated water. From the theory, the solubility of oxygen in water will
decrease with increasing temperature. Aerated water will have higher oxygen content than
unaerated water. The error caused might due to the limitations stated above.

REFERENCES

1. J. Oncol. (2008, Jan). Measurement of Dissolved Oxygen Using Invasive Oxygen


Sensitive Electrode. PubMed. Retrieved on July 23, 2019 from
https://www.ncbi.nlm.nih.gov/pubmed/18097544

2. Jorge G. Ibanez. (2008, Jan). Dissolved Oxygen in Water. Environmental Chemistry


ResearchGate. Retrieved on July 23, 2019 from
https://www.researchgate.net/publication/251263393_Dissolved_Oxygen_in_Water

3. Fondriest Environmental Learning Center. Measurements and Parameter of Water


Quality. Retrieved on July 23, 2019 from https://www.fondriest.com/environmental-
measurements/parameters/water-quality/water-temperature/

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