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Basic Haematological Techniques: Carol Briggs - Barbara J. Bain

This document provides an outline of basic hematological techniques used to analyze blood samples, including methods for measuring hemoglobin concentration, packed cell volume, platelet count, reticulocyte count, red and white blood cell counts, and differential white blood cell counts. Automated blood counting techniques are discussed alongside traditional manual methods. Ranges of normal results are provided for many of the tests.

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Rahmat Satriadi
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131 views

Basic Haematological Techniques: Carol Briggs - Barbara J. Bain

This document provides an outline of basic hematological techniques used to analyze blood samples, including methods for measuring hemoglobin concentration, packed cell volume, platelet count, reticulocyte count, red and white blood cell counts, and differential white blood cell counts. Automated blood counting techniques are discussed alongside traditional manual methods. Ranges of normal results are provided for many of the tests.

Uploaded by

Rahmat Satriadi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Basic Haematological
Techniques
Carol Briggs  •  Barbara J. Bain

CHAPTER OUTLINE
Haemoglobinometry,  19 Platelet count,  27
Measurement of haemoglobin concentration Range of platelet count in health,  27
using a spectrometer (spectrophotometer) or Reticulocyte count,  27
photoelectric colorimeter,  19 Reticulocyte stains and count,  27
Haemiglobincyanide (cyanmethaemoglobin) Fluorescence methods for performing a
method,  20 reticulocyte count,  30
Diluent,  20 Manual reference method,  30
Haemiglobincyanide reference standard,  20 Range of reticulocyte count in health,  30
Method,  21 Automated blood count techniques,  30
Calculation of haemoglobin concentration,  21 Haemoglobin concentration,  31
Direct spectrometry,  22 Red blood cell count,  31
Direct reading portable haemoglobinometers,  22 Counting systems,  31
Colour comparators,  22 Impedance counting,  31
Portable haemoglobinometers,  22 Light scattering,  32
Noninvasive screening tests,  23 Reliability of electronic counters,  32
Range of haemoglobin concentration in Setting discrimination thresholds,  33
health,  23
Packed cell volume and mean cell
Packed cell volume or microhaematocrit,  23 volume,  33
International Council for Standardisation in Red cell indices,  35
Haematology reference method,  24
Mean cell volume,  35
Surrogate reference method,  24
Mean cell haemoglobin and mean cell
Range of packed cell volume in health,  24 haemoglobin concentration,  35
Manual cell counts and red cell indices,  24 Variations in red cell volumes: red cell
Range of MCHC in health,  25 distribution width,  35
Manual differential leucocyte count,  25 Percentage hypochromic red cells and variation
Method,  25 in red cell haemoglobinisation: haemoglobin
Basophil and eosinophil counts,  26 distribution width,  36
Range of eosinophil count in health,  26 White blood cell count,  36
Range of basophil count in health,  26 Automated differential count,  37
Reporting the differential leucocyte count,  26 The automated immature granulocyte count,  38
Reference differential white cell count,  27 The automated nucleated red blood cell
Range of differential white cells in health,  27 count,  38

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