Leica ST4040

Linear Stainer

Instruction Manual
Leica ST4040 V1.4 - Rev A, English – 06/2009
Always keep this manual near the instrument.
Read carefully prior to operating the instrument.
 IMPORTANT NOTE

The information, numerical data, notes and val- ucts. These are determined only by the contract
ue judgments contained in this manual repre- provisions agreed between ourselves and our
sent the current state of scientific knowledge customers.
and state-of-the-art technology as we under- Leica reserves the right to change technical
stand it following thorough investigation in this specifications as well as manufacturing pro-
field. We are under no obligation to update the cesses without prior notice. Only in this way is it
present manual periodically and on an ongoing possible to continuously improve the technolo-
basis according to the latest technical develop- gy and manufacturing techniques used in our
ments, nor to provide our customers with addi- products.
tional copies, updates etc. of this manual. This document is protected under copyright
For erroneous statements, drawings, technical laws. Any copyrights of this document are re-
illustrations etc. contained in this manual we tained by Leica Biosystems Nussloch GmbH.
exclude liability as far as permissible according Any reproduction of text and illustrations (or of
to the national legal system applicable in each any parts thereof) by means of print, photocopy,
individual case. In particular, no liability what- microfiche, web cam or other methods – includ-
soever is accepted for any financial loss or con- ing any electronic systems and media – re-
sequential damage caused by or related to quires express prior permission in writing by Le-
compliance with statements or other informa- ica Biosystems Nussloch GmbH.
tion in this manual. For the instrument serial number and year of
Statements, drawings, illustrations and other in- manufacture, please refer to the name plate at
formation as regards contents or technical de- the back of the instrument.
tails of the present manual are not to be consid-
ered as warranted characteristics of our prod- © Leica Biosystems Nussloch GmbH

Issued by:

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19
D-69226 Nussloch Serial No.: .............. ......................................................
Germany
Year of Manufacture: ...............................................
Phone: +49 6224 143-0
Fax: +49 6224 143-268 Country of Origin: Federal Republic of Germany
Internet: http://www.microsystems.com

Leica ST4040 – Linear Stainer 3

Table of Contents

IMPORTANT NOTE ........................................................................................................................................................................................... 3

1. Important Information .......................................................................................................................................................................... 6
1.1 Symbols used in this manual and ....................................................................................................................................................... 6
1.2 Designated use ...................................................................................................................................................................................... 7

2. Safety ....................................................................................................................................................................................................... 8
2.1 Safety instructions ................................................................................................................................................................................ 8
2.1.1 Transport and installation ................................................................................................................................................................... 8
2.1.2 Operating the instrument ..................................................................................................................................................................... 9
2.1.3 Cleaning and maintenance ............................................................................................................................................................... 10

3. Installation ........................................................................................................................................................................................... 11
3.1 Site requirements ................................................................................................................................................................................ 11
3.2 Unpacking the instrument .................................................................................................................................................................. 12
3.2.1 Repacking the instrument ................................................................................................................................................................. 12
3.3 Installing the instrument ..................................................................................................................................................................... 13
3.4 Standard delivery ‘basic instrument’ and general accessories ................................................................................................. 14
3.4.1 Standard delivery ................................................................................................................................................................................ 14
Basic instrument ST4040 - single load model ................................................................................................................................ 14
Basic instrument ST4040 - double load model (for double row staining) ................................................................................. 14
3.4.2 General accessories .......................................................................................................................................................................... 15
3.5 Electrical connections ........................................................................................................................................................................ 16
3.5.1 Adjusting the voltage selector .......................................................................................................................................................... 16
3.5.2 Connecting the power chord to the ................................................................................................................................................. 17
3.6 Installing the accessories .................................................................................................................................................................. 17
3.6.1 Installing the water inlet hose .......................................................................................................................................................... 17
3.6.2 Installing the drain hose .................................................................................................................................................................... 17
3.6.3 Installing the exhaust air hose ......................................................................................................................................................... 17
3.6.4 ..................................................................................................................................................................... Placing the instrument in its
........................................................................................................................................................................................................................
..................................................................................................................................................................................... permanent position
18
3.6.5 Connecting the inlet hose to the tap ................................................................................................................................................ 18
3.6.6 Leveling the instrument ..................................................................................................................................................................... 18
3.6.7 Inserting the activated carbon filter ................................................................................................................................................ 19
3.7 Plugging the power chord into
...................................................................................................................................................................................................... the wall outlet
19

4. Installation of the Optional Load/Unload Station ......................................................................................................................... 20

4.1 Site requirements ................................................................................................................................................................................ 20
4.2 Unpacking the optional load / unload station ....................................................................................................................... 20
4.2.1 Repacking the optional load / unload station ...................................................................................................................... 20
4.3 Standard delivery and accessories - optional load / unload station .......................................................................................... 21
4.3.1 Standard delivery - optional load station ......................................................................................................................................... 21
Accessories - optional load station ................................................................................................................................................. 21
4.3.2 Standard delivery - optional unload station ................................................................................................................................... 21

4 Instruction Manual V 1.4 – 06/2009

 Table of Contents

Accessories - optional unload station ............................................................................................................................................ 21

4.4 Installation ............................................................................................................................................................................................ 22
4.4.1 Installing the optional load station ................................................................................................................................................... 22
4.4.2 Installing the optional unload station ..................................................................................................................................................
on the right of the basic instrument ..................................................................................................................................................... 25
4.5 Changing the guide ramps for slide rack carriers ......................................................................................................................... 28

5. Instrument Properties ........................................................................................................................................................................ 29

5.1 Technical Data ..................................................................................................................................................................................... 29

6. Operation .............................................................................................................................................................................................. 30
6.1 Setting up the instrument ................................................................................................................................................................... 30
6.2 Preparing the instrument for staining: inserting and filling the stations .................................................................................... 30
6.3 Staining in two rows ........................................................................................................................................................................... 31
6.3.1 Doubling the specimen throughput ................................................................................................................................................. 31
6.3.2 Carrying out two different, but matching, staining protocols simultaneously ......................................................................... 31
6.3.3 Carrying out two different staining protocols (two subsequent single loads) ......................................................................... 31
6.4 Control panel functions ...................................................................................................................................................................... 32
6.5 Switching on mains and selecting direction of travel and alarm volume .................................................................................. 33
........................................................................................................................................................................................................................... 33
6.5.1 Selecting direction of travel .............................................................................................................................................................. 33
........................................................................................................................................................................................................................... 33
6.5.2 Selecting alarm volume ..................................................................................................................................................................... 34
........................................................................................................................................................................................................................... 34
6.6 Switching on mains without selecting direction of travel/alarm volume .................................................................................. 34
........................................................................................................................................................................................................................... 34
6.6.1 Stand-by mode .................................................................................................................................................................................... 34
........................................................................................................................................................................................................................... 34
6.7 Programming ........................................................................................................................................................................................ 35
6.8 Staining ................................................................................................................................................................................................. 36
6.8.1 Selecting a program ........................................................................................................................................................................... 36
........................................................................................................................................................................................................................... 36
6.8.2 Preparing and inserting the slide racks .......................................................................................................................................... 37
6.8.3 Starting the selected program .......................................................................................................................................................... 38
........................................................................................................................................................................................................................... 38
6.9 Removing the slide racks ................................................................................................................................................................... 39
6.8.4 Interrupting a program ....................................................................................................................................................................... 39
........................................................................................................................................................................................................................... 39
6.9.1 Removing the last slide rack ............................................................................................................................................................. 40
........................................................................................................................................................................................................................... 40
6.10 Removing the slide racks in instruments equipped with optional unload station ................................................................... 40
........................................................................................................................................................................................................................... 40
6.11 Finishing work ..................................................................................................................................................................................... 41

........................................................................................................................................................................................................................... 41

7. Trouble Shooting ................................................................................................................................................................................. 42

8. Cleaning and Maintenance .............................................................................................................................................................. 44
8.1 Cleaning ................................................................................................................................................................................................ 44
8.2 Maintenance ........................................................................................................................................................................................ 46
Leica ST4040 – Linear Stainer 5
9. Warranty and Service ........................................................................................................................................................................ 47
1. Important Information

The instruction manual for the Leica 1.1 Symbols used in this manual and
ST4040 includes chapters on the following their meaning
subjects:
Chapter 1 Structure of this manual Warnings appear in a grey box and are
• Table of contents marked by a warning triangle.

• Important information on this

manual
Chapter 2 Safety Notes
• Read this chapter before i.e. important user information appear
in a grey box and are marked by an in-
operating the instrument
formation symbol.
Chapter 3 Installation
• Unpacking and installation
• Standard delivery, accessory
(5) Figures in brackets refer to item num-
range bers in drawings or to the drawing it-
Chapter 4 Installation (Fig. 5) self.
Optional load / unload station
• Unpacking and installation
Instrument type:
• Standard delivery, accessory
range All information given in this instruction manual
Chapter 5 Instrument features applies only to the instrument type indicated on
the title page.
• Technical data
A name plate indicating the instrument serial
Chapter 6 Operation
number is attached to the back of the instru-
• Controls ment.
• Setup and daily use
Chapter 7 Trouble Shooting Required information for all enquiries:
• Operating errors For any enquiries please specify:
• Trouble shooting • Instrument type
Chapter 8 Cleaning and Maintenance • Serial number
Chapter 9 Warranty and Service
Chapter 10 EC Declaration of Conformity
Appendix 1 Staining protocols Leica ST4040

6 Instruction Manual V1.4 – 06/2009

 1. Important Information

General 1.2 Designated use

This instruction manual includes important in- The Leica ST4040 is an automated linear stainer
structions and information related to the operat- for the preparation of histological and cytologi-
ing safety and maintenance of the instrument. cal routine stainings.
The instruction manual is an important part of It is designed for use in pathology laboratories,
the product. It must be read carefully before and only for performing the following tasks:
first using the instrument and must always be
kept with the instrument. • Staining of thin sections of tissue specimens
or of cytological samples, attached to micro-
It will be necessary to add appropriate instruc- scope slides
tions to this instruction manual, if imposed by
existing national regulations or by laws on acci-
dent prevention and environmental protection in Any use of the instrument other than its de-
the country of the operating authority. signated use is considered improper.

Read this instruction manual carefully before

attempting to use or operate the instrument.

Please pay particular attention to the

safety instructions in chapter 2. Please
do read this information, even if you
are familiar with the operation and use
of Leica products.

Leica ST4040 – Linear Stainer 7

2. Safety

2.1 Safety instructions 2.1.1 Transport and installation

This instrument has been built and tested in ac- • Do not operate the instrument in rooms
cordance with the following safety regulations where risk of explosion exists!
on electrical measuring, control, regulating and
laboratory devices.
• Do not expose the instrument to direct sun-
In order to maintain this condition and to ensure light (windows)!
safe operation, the operator must observe the
instructions and warnings contained in this in-
struction manual. • Do not install the instrument above a heater!
For current information about applicable stan-
dards, please refer to the CE declaration of con- • Install the instrument on an even laboratory
formity on our Internet site: bench which must be absolutely level!
www.histo-solutions.com
• Two people are needed to lift / carry the in-
strument!

• Before connecting the instrument to mains,

make sure the correct voltage setting,
matching the nominal voltage at the site of
installation, has been selected!

• Upon installing the drain hose make sure

there is a gradient from the drain outlet to
the waste pipe.

• To protect the user from hazardous solvent

fumes, make sure to operate the instrument
either with the activated carbon filter or the
exhaust air hose!

8 Instruction Manual V1.4 – 06/2009

 2. Safety

2.1.2 Operating the instrument

• The instrument may only be operated by • If not only the water tap but also the ball
skilled personnel. It may only be operated valve has been closed (--> e.g. when setting
according to its designated use and in ac- up the instrument), the tap water flow rate
cordance with the instructions given in this has to be adjusted when starting a program
manual! (see also chapters 3.6 and 6.8.3).

• While working with reagents (filling / emp-ty- • The flow rate must not be too fast, to en-sure
ing the reagent stations, working on the in- that the specimens remain firmly at-tached
strument while the lid/s is/are open) appro- to the slide surface!
priate protective gear (lab coat, gloves, safe-
ty goggles) must be worn!
• If a staining program is to be interrupted for
an extended period of time, do not leave any
• Make sure to operate the instrument ei-ther slide racks in the tap water sta-tions, in order
with the activated carbon filter or with the to prevent them from drying out!
exhaust air hose (--> Chapter 3.6, ‘Installing
the accessories’). Even when the instrument
• As soon as the alarm is triggered, immediate-
is operated according to its designated use,
ly remove the completed slide rack from the
hazardous solvent fumes develop, which are
last station or from the unload container of
damaging to the operator’s health and do
the optional unload station! Otherwise, im-
also pose a risk of fire!
mersion times for the remaining slide racks
will be prolonged!
• Risk of fire, when working with an open
flame (Bunsen burner) immediately next to
• In case of emergency switch off mains and
the instrument (solvent fumes)! - Therefore,
unplug the power chord!
keep a safety distance of 1 meter!

• If ‘Alarm off’ is selected (not recommend-

ed!), the instrument must be observed con-
stantly, to make sure all slide racks are re-
moved immediately when reaching the last
station!

Leica ST4040 – Linear Stainer 9

2. Safety

2.1.3 Cleaning and maintenance

• Only technical service engineers authorized • When handling cleaning detergents, follow
by Leica may access the internal compo- the instructions of the manufacturer and
nents of the instrument for service and re- make sure all applicable laboratory regula-
pair. tions are complied with!
Exception: Changing the activated carbon fil-
ter is the only maintenance task to be car- • When cleaning the instrument, no liquid may
ried out by the user. enter in contact with any of the electrical
connections or get into the interior of the in-
• Prior to cleaning the instrument, always strument!
switch off mains and unplug the power
chord! • Wash the tap water and reagent stations in
the dishwasher at a temperature of max. +65
• Dispose of used reagents according to the °C. - Use a standard detergent for laboratory
laboratory regulations in force in your coun- dishwashers.
try! At any rate avoid washing the stations at
higher temperatures (e. g. in industrial dis-
• Spilled solvents (reagents) have to be wiped washers which run at a temperature of
away immediately! - In case of long-term ex- +85 °C), as the stations may become de-
posure, the lid surfaces are only conditional- formed!
ly resistant to solvents!

• The painted surfaces and the control panel

are not resistant to xylene or acetone!

• For cleaning the instrument, do not use any

one of the following: alcohol, detergents
containing alcohol (window cleaner!), abra-
sive cleaning powders, solvents containing
xylene or acetone!

• To clean the lids, control panel and housing,

use mild household detergents; - see safety
instruction above for non-appropriate ingre-
dients!

10 Instruction Manual V1.4 – 06/2009

 3. Installation

3.1 Site requirements

The installation site has to meet the following
requirements: Do not operate the instrument in rooms
where risk of explosion exists!
• Stable laboratory bench, exactly level, at
Do not expose the instrument to direct
least 1.60 m wide and 60 cm deep. sunlight (windows)!
• Tap water supply at a distance of max. 2 m
and drain at a distance of max. 1.50 m from Do not install the instrument above a
heater!
the corresponding inlet and outlet at the
back of the instrument.
- Please bear in mind: The connections are
located at the extreme left on the back of the
instrument.
• Fume hood at a distance of max. 3.50 m from
the instrument, if the instrument is to be op-
erated with the exhaust air hose (otherwise
the instrument must be operated with the
activated carbon filter)!
• Vibration-free floor.
• Sufficient open space (70 cm) above the lab-
oratory bench, to ensure trouble-free open-
ing of the lids.
• Stable ambient temperature of +10 °C to +35
°C.
• Relative air humidity of maximum 80%, non-
condensing.
• No other instruments which cause vibra-
tions installed nearby.

Leica ST4040 – Linear Stainer 11

3. Installation

3.2 Unpacking the instrument 3.2.1 Repacking the instrument

The unpacking instructions for all Leica ins- We recommend keeping the original crate and
truments are located in a transparent pro- packing material in store, in case the ins-
tective envelope on the outside of the instru- trument needs to be repacked in the future.
ment transportation crates.
The drawing below shows the design of the
original crate and packing material. The num-
bers indicate the sequence of disassembling
and reassembling the crate.

7
6

3
2

12 Instruction Manual V1.4 – 06/2009

 3. Installation

3.3 Installing the instrument

• To lift, grip the instrument by the transport
handles.

Two people are needed to lift / carry

the instrument, since it weighs 73 kg
(see ‘Technical data’, chapter 5.1)!

• Install the instrument on the designated

bench.

• Unscrew the transport handles.

• Remove the plastic protective cover from the

instrument.

• Check all delivered parts against the packing

list to verify whether the delivery is complete
- see chapter 3.4 ‘Standard delivery ‘basic
instrument’ and general accessories’).

• For all further installation steps, see chap-

ters 3.5 ‘Electrical connection’ and 3.6 ‘In-
stalling the accessories’.

Leica ST4040 – Linear Stainer 13

3. Installation

3.4 Standard delivery ‘basic instrument’ and general accessories

3.4.1 Standard delivery
Basic instrument ST4040 - single load model
• Basic instrument with three-piece lid
• 27 Reagent stations, plastic
• 4 tap water stations, complete assy. with inlet valve
• 1 Drain hose for waste water, 2 m long
• 1 Tap water inlet hose, 2.50 m long,
complete with 3/4“ connection for water tap and replacement gasket
• 2 Lids for reagent stations
• 1 Cover for second row
• 1 Instruction manual ................................................................ 14 0474 80001
• 1 Tool set, including:
• 1 Single-ended open-jaw wrench, size 27
• 1 Single-ended open-jaw wrench, size 13
• 1 Screw driver, 5.5 x 200 mm
• 1 Screw driver, 3 x 50 mm
• 1 Water tap adapter for 1/2“ water taps
• 3 Disposable plastic films, solvent-resistant, for control panel
• 1 Set of power chords:
• Euro
• UK
• USA

Basic instrument ST4040 - double load model (for double row staining)

To use a single load model for double row staining, order the appro-
priate number (may vary - depends on individual staining applica-
tions) of the accessories listed below (see chapter 3.4.2 - ‘General
accessories’ for details):

• Reagent stations
• Tap water stations
• Lids for reagent stations
• Slide racks
• Slide rack carriers

14 Instruction Manual V 1.4 – 06/2009

 3. Installation

3.4.2 General accessories

• Reagent stations, plastic
• Tap water stations, complete assy. with inlet valve
• Leica slide racks, metal
• Sakura slide racks, plastic
• Adapter for large slides
• Drain hose, 4 m long
• Tap water inlet hose, 2.50 m long,
complete with 3/4“ connection for water tap
• Lids for reagent stations
• Slide rack carriers for Leica slide racks
• Slide rack carriers for Medite/Hacker slide racks
• Slide rack carriers for Sakura slide racks
• Storage container for slide rack carriers, fits on instrument housing
• Activated carbon filter
• Exhaust air hose, 2 m long
• Exhaust air hose, 4 m long
• Disposable plastic films, solvent-resistant, for control panel (set of 10)
• Optional load station, left*
• Optional load station, right*
• Optional unload station, left*
• Optional unload station, right*
• Leica CV5000 adapter for slide racks:
• CV5000 slide rack adapter, removable:
- slides onto Leica slide racks for coverslipping in Leica CV5000.
- up to Leica CV5000 s/nos. ≤ CV026096
• CV5000 stationary slide rack adapter:
- for permanent installation in Leica CV5000 for using Leica
ST4040 slide racks in CV5000.
- starting from Leica CV5000 s/nos. > CV026096)

To order accessories, please contact your Leica local sales organi-

zation for the latest literature / current order numbers on the full
range of Leica ST4040 accessories.

*) For accessories for optional load / unload station, please refer to

chapters 4.3.1 and/or 4.3.2 respectively!

Leica ST4040 – Linear Stainer 15

3. Installation

3.5 Electrical connections

3.5.1 Adjusting the voltage selector • Insert the small
screwdriver (3 x 50 mm)
The voltage selector is preset to match into the notch at the
the nominal voltage of the country of lower end of the voltage
delivery. selector shell. Carefully
Nevertheless, it is absolutely neces- release the lock, using
sary that you check the setting of the the screwdriver as a le-
voltage selector prior to connecting
ver.
the instrument to mains, to make sure
the setting is correct!

Connecting the instrument to mains

with the voltage selector at a wrong 3
setting can cause severe damage to
the instrument!
2

• Check the setting showing in window (1).

• Remove shell (2) with fuses (3).
• Take the fuses out of the shell.
1 • Pull the voltage selector out of the shell and
reinsert it such that the desired setting is
visible in the small window (1) of the shell.
• Reinsert the shell together with voltage se-
lector and fuses into the corresponding
• Does it correspond to the nominal voltage in opening at the back of the instrument and
your laboratory? apply light pressure until it locks.

• If the setting is correct: --> Go to next page

1
(chapter 3.5.2).

• If the setting is not correct, the voltage se-

lector must be changed to the correct
setting: --> Continue to follow instructions
on this page. • Double-check if the correct setting now
shows in window (1).

16 Instruction Manual V1.4 – 06/2009

 3. Installation

3.5.2 Connecting the power chord to the 3.6 Installing the accessories
instrument
3.6.1 Installing the water inlet hose

• Remove the adhesive tape from the mains

inlet.
• Install the inlet hose, which supplies water
to the tap water stations.

3.6.2 Installing the drain hose

• Select the appropriate power chord (the in-

strument is delivered with several, country-
• Connect the drain hose.
specific power chords) and connect it to the
mains inlet at the rear of the instrument. Upon installing the drain hose make
• Do not yet plug the power chord into the wall sure there is a gradient from the drain
outlet. outlet to the waste pipe.

3.6.3 Installing the exhaust air hose

• The unload sensor port remains empty; un- • Connect the exhaust air hose (optional!).
less the instrument is operated with optional The instrument may be operated ei-
unload station (see Chapter 4). ther with the exhaust air hose or
with the activated carbon filter.

Leica ST4040 – Linear Stainer 17

3. Installation

3.6.4 Placing the instrument in its

permanent position
• Place the instrument in its permanent posi-
Ball valve closed.
tion on the laboratory bench.
• Connect the exhaust air hose to the fume
hood, or lead outside.
• Insert drain hose into drain. • Do not yet open neither water tap nor ball
valve (see above)!
3.6.5 Connecting the inlet hose to the tap
When setting up the instrument, an ad-
justment of the tap water flow rate has
to be done. This adjustment cannot be
done until after the tap water stations
have been inserted. - For detailed in-
structions on the flow rate adjustment,
see Chapter 6.8.3!

For safety reasons, we recommend us-

• Connecting to 3/4“ water tap: ing a so-called ‘Aquastop’ device, as
commonly used with household appli-
Connect the ball valve to the water tap and
ances.
attach the tap water inlet hose to the ball
valve.
3.6.6 Leveling the instrument

When installed in its final posi-tion on

the laboratory bench, the instrument
must be exactly level! (see also chap-
ter 3.1 ‘Site requirements’).

• If necessary, the instrument can be slightly

readjusted via the adjustable instrument
• Connecting to 1/2“ water tap: feet.
Fit the adapter in between water tap and • For that purpose, screw the instrument feet
ball valve. in or out, as needed, until the instrument is
positioned horizontally.

18 Instruction Manual V1.4 – 06/2009

 3. Installation

3.6.7 Inserting the activated carbon filter

If the instrument is not connected to a

fume hood (via exhaust air hose), an
activated carbon filter must be used!

• Insert the activated carbon filter.

• Close the front panel. While applying light
pressure to the front panel, press the two
locking pins to lock the panel into place.

• To insert the activated carbon filter, open the 3.7 Plugging the power chord into
hinged front panel: press the two locking pins, the wall outlet
which are located on the top right and left on
the inside of the front panel.

• Remove the the activated carbon filter from • Prior to plugging the power chord into the
the package. wall outlet, check whether the mains switch
• Write the current date on the filter (to be re- is in OFF (‘0’) position.
minded to replace the filter in time). • Connect the power chord to the wall outlet.

Leica ST4040 – Linear Stainer 19

4. Installation of the Optional Load/Unload Station

4.1 Site requirements 4.2.1 Repacking the optional load / unload

station
The installation site has to meet the following
requirements: We recommend keeping the original crates and
• Stable laboratory bench, exactly level, at packing material in store, in case the load / un-
least 1.60 m wide and 60 cm deep for the ba- load stations need to be repacked in the future.
sic instrument, plus an additional 0.30 m of The drawing below shows the design of the
width for each of the optional stations. original crate and packing material. The num-
bers indicate the sequence of disassembling
• Other than that, the same site requirements
and reassembling the crate.
as listed for the basic instrument apply (see
Chapter 3.1).

4.2 Unpacking the optional load / 7

unload station
The unpacking instructions for all Leica ins-
truments are located in a transparent pro- 6
tective envelope on the outside of the
instrument transportation crates.
5

3
2

20 Instruction Manual V1.4 – 06/2009

 4. Installation of the Optional Load/Unload Station

4.3 Standard delivery and accessories - optional load / unload station

4.3.1 Standard delivery - optional load station
• 1 Optional load station
• 5 Reagent stations
• 1 lid for reagent stations of optional load station
• 1 Tool set, including:
• 1 Hexagon key, size 2.5
• 1 Hexagon key, size 3
• 1 Hexagon key, size 4
• 1 Ring wrench, size 10

Accessories - optional load station

• Reagent stations
• Lid for reagent stations of optional load station

4.3.2 Standard delivery - optional unload station

• 1 Optional unload station
• 1 Unload container for optional unload station
• 1 Lid for unload container of optional unload station
• 1 Connecting cable for unload sensor
• 1 Pair of guide ramps no. 2, for slide racks / slide rack carriers
by Medite/Hacker and/or Sakura
• 1 Tool set, including:
• 1 Hexagon key, size 1.5
• 1 Hexagon key, size 2.5
• 1 Hexagon key, size 3
• 1 Hexagon key, size 4
• 1 Ring wrench, size 10

Accessories - optional unload station

• Unload container for optional unload station*
• Lid for unload container of optional unload station

For double loading (staining in two rows) with optional load and/or
unload station attached to the basic instrument, order the necessary
accessories for the optional load and/or unload station as needed.

*) Can also be used for intermediate storage of slide racks prior to

coverslipping; (see chapter 6.10 for details).

Leica ST4040 – Linear Stainer 21

4. Installation of the Optional Load/Unload Station

4.4 Installation
4.4.1 Installing the optional load station
on the left side of the basic instrument

• Remove the side panel from the side wall

and detach the ground wire from the side
wall.
• Remove the 4 hexagon socket screws from
the left lid (hexagon key, size 4).

• Detach the ground wire and pull it out of the

instrument (will not be needed any longer.)
• Remove the lid. - Keep the screws (will be
needed later)!

• Press the Start/Stop button, to move the

conveyor frame to the upper position. As
• Remove the 2 black screws from the left side
soon as the conveyor frame has reached the
panel (hexagon key, size 3).
upper end position, switch the instrument off
(ON/OFF button).

22 Instruction Manual V1.4 – 06/2009

 4. Installation of the Optional Load/Unload Station

• Place the optional load station on the left • Plate (1) which connects optional load station
side of the basic instrument. and basic instument is fastened with two Al-
lan screws.

In order to establish safe protective

grounding, it is essential to fasten plate
(1) securely.

• Move the optional load station right up to the

instrument, inserting the two pins, located
on the left and right at the bottom of the op-
tional load station, into the corresponding
openings in the basic instrument.
• Open the Lid

• Tighten the screw on the left wall of the op- • Pull the conveyor frame of the optional load
tional load station ring wrench, size10). station upwards using both your hands.

Leica ST4040 – Linear Stainer 23

4. Installation of the Optional Load/Unload Station

• Lower the conveyor frame (now connected

to one single piece) pushing the ON/OFF but-
ton.
• Fit the connecting plates attached to the
front and rear half of the conveyor frame of
the optional load station over the insides of
the front and rear half of conveyor frame of
the basic instrument.

• Use the 4 hexagon socket screws which you

have kept (see step 1) to attach the lid of the
optional load station to the corresponding
hinge (= left hinge of basic instrument).

• Bolt the two front halves of the conveyor To install an optional load station to
frames together with two hexagon socket the right side of the basic instrument,
screws (hexagon key, size 2.5). follow the same steps - carried out lat-
erally reversed.

• Bolt the two rear halves of the conveyor

frames together with two hexagon socket
screws (hexagon key, size 2.5).

24 Instruction Manual V1.4 – 06/2009

 4. Installation of the Optional Load/Unload Station

4.4.2 Installing the optional unload station

on the right of the basic instrument

• Remove the side panel from the side wall

and detach the ground wire from the side
• Remove the 4 hexagon socket screws from wall.
the right lid (hexagon key, size 4).

• Detach the ground wire and pull it out of the

• Remove the lid. - Keep the screws (will be instrument (will not be needed any longer.)
needed later)!

• Press the Start/Stop button, to move the

• Remove the 2 black screws from the right conveyor frame to the upper position. As
side panel (hexagon key, size 3). soon as the conveyor frame has reached the
upper end position, switch the instrument off
(ON/OFF button).

Leica ST4040 – Linear Stainer 25

4. Installation of the Optional Load/Unload Station

In order to establish safe protective

grounding, it is essential to fasten
plate (1) securely.

• Place the optional unload station on the right

side of the instrument.
• Move the optional unload station right up to
the instrument, inserting the two pins, locat-
ed on the left and right at the bottom of the
optional load station, into the corresponding
openings in the basic instrument.

• Open the right lid.

• Tighten the screw on the right wall of the op-

tional unload station (ring wrench, size10).

• Pull the conveyor frame of the optional un-

1
load station upwards using both your hands.
• Fit the connecting plates attached to the
front and rear half of the conveyor frame of
the optional unload station over the insides
• Plate (1) which connects optional unload of the front and rear half of the conveyor
station and basic instument is fastened with frame of the basic instrument.
two Allan screws.

26 Instruction Manual V1.4 – 06/2009

 4. Installation of the Optional Load/Unload Station

• Bolt the two front halves of the conveyor • Use the 4 hexagon socket screws which you
frames together with two hexagon socket have kept (see step 1) to attach the lid of the
screws (hexagon key, size 2.5). optional unload station to the corresponding
hinge (= right hinge on basic instrument).

• Bolt the two rear halves of the conveyor

frames together with two hexagon socket
screws (hexagon key, size 2.5).

• Insert the plug of the unload sensor cable

into the unload sensor port on the rear of the
basic instrument.

To install an optional unload station on

the left side of the basic instrument,
follow the same steps - carried out lat-
erally reversed.
• Lower the conveyor frame (now connected
to one single piece) pushing the ON/OFF
button.

Leica ST4040 – Linear Stainer 27

4. Installation of the Optional Load/Unload Station

4.5 Changing the guide ramps for slide rack carriers

To operate the instrument with Medite

/ Hacker or Sakura slide racks, the
standard guide ramps no. 1 for Leica
slide racks must be exchanged for
guide ramps no. 2 (see ‘Standard deliv-
2
ery - optional unload station’, chapter
4.3.2).

• Place grooved ramp no. 2 onto the rear pin

and use the hexagon key, size 1.5 to fasten
the two grub screws in the ramp (clockwise
rotation through about 180 °).

1 • Place plain ramp no. 2 onto the front pin and

fasten the front ramp (clockwise rotation of
both grub screws through about 180 ° -
• Use the hexagon key, size 1.5 to unscrew hexagon key, size 1.5).
both ramps no. 1 loosening the two corre-
sponding grub screws in each ramp (coun-
terclockwise rotation through about 180 °). Store ramps no. 1 for future use!
Remove both ramps.

28 Instruction Manual V1.4 – 06/2009

 5. Instrument Properties

5.1 Technical Data

General
Admissions: VDE, UL, cUL, C-Tick Label
Nominal supply voltage: available: 100 V AC ± 10 %
120 V AC ± 10 %
230 V AC ± 10 %
240 V AC ± 10 %
Nominal frequency: 50/60 Hz
Maximum power draw: 150 VA
1
Protective class : I
Mains fuses: Circuit breaker manufactured by ETA, 2 A
model: 3120-F421-P7T1-W01D-2 A
Primary fuses: manufactured by Schurter: model Fst 2 x T8 A
Secondary fuses: manufactured by Schurter: model Fst Motor (F1) T 600 mA
Electronic (F2) T 1,6 A
1
Pollution degree : 2
Overvoltage installation category: II
Working temperature range: +10 °C to +35 °C
Relative air humidity: max. 80 %, non-condensing
Sound intensity level: < 70 dB
1)
according to IEC-1010, UL 3101, EN 61010

Dimensions and weight

Basic instrument (W x H x D): 1,435 x 444 x 436 mm
Basic instrument with optional load and unload station (W x H x D): 1,969 x 444 x 436 mm
Optional load / unload station (W x H x D): 267 x 444 x 392 mm
Working height: 318 mm
Weight: (Basic instrument with accessories) 73 kg
(optional load station) 16 kg
(optional unload station) 14 kg

Leica ST4040 – Linear Stainer 29

6. Operation

6.1 Setting up the instrument

To set up the instrument follow all instructions of chapter 6 step by

step.

6.2 Preparing the instrument for staining: inserting and filling the stations
• Select a staining protocol.
(For examples, see Appendix 1: ‘Staining protocols’)

• According to the selected staining protocol, determine the order of re-

agent and tap water stations.

• Open all three lids.

The Leica ST4040 is equipped with a three-piece lid, thus e n -

abling the operator to open just one of the two small lids on the right
or left of the instrument for loading / unloading slide racks. This sys-
tem keeps the exposure of laboratory personnel to hazardous fumes
to a minimum.

• Remove the cover screws from those positions where you have decid-
ed to install tap water stations (tap water stations can be installed from
positions 3 through 20).

• Insert the tap water stations (yellow - see left) and the reagent stations
(white).

• Make sure that all stations are inserted correctly; the stations must not
be jammed (overlapping rims!).
• Add reagents according to selected staining protocol. - Do not exceed
the maximum volume or remain under the minimum volume!
• Close the lid entirely.

30 Instruction Manual V 1.4 – 06/2009

 6. Operation

6.3 Staining in two rows

6.3.1 Doubling the specimen throughput
• For this type of application, the sequences of reagent / tap water sta-
tions in rows 1 and 2 must be identical.

• According to the station sequence selected for row 1 (see Chapter 6.2)
insert an identical sequence in row 2.

• Both rows are run with the same program.

6.3.2 Carrying out two different, but matching, staining protocols simultaneously
• Precondition: the program parameters of both protocols must be the
same (i.e. both rows are run with the same program).

• The two staining protocols are coordinated through the number of re-
agent stations and/or the concentration or dilution of the reagents (for
details, see Appendix 1 - ‘Staining protocols’).

Important note to chapters 6.3.1 and 6.3.2

• When staining in two rows, standard slide rack carriers, as for single
row staining, are used.

• Slide rack carriers located side by side in rows 1 and 2 are inserted
laterally reversed into the same notch of the conveyor frame (see left).

6.3.3 Carrying out two different staining protocols (two subsequent single loads)
• The two rows are never carried out simultaneously, i.e.: row 1 is run
with program 1 - row 2 with program 2, or vice versa.

In 6.3.3 the advantage of staining in two rows is that 2 different staining protocols, though not
run simultaneously, can be run one directly after another without any delay; whereas in a sin-
gle load model, stations would have to be rearranged, refilled etc. prior to being able to run the
second protocol.

Leica ST4040 – Linear Stainer 31

6. Operation

6.4 Control panel functions

IMMERSION TIME DRAINING TIME ON/OFF button -

button - button - switch on instrument
Display / select im- Display / select --> stand-by mode
mersion time draining time

DISPLAY AGITATION button - MAINS SWITCH -

SCREEN- Agitation (dips) connects / disconnects
Display immersion ON/OFF instrument to /
and draining times from mains
(0 = OFF / I = ON)

PROGRAM 1 button - START/ STOP button -

Select / program Start / stop
program no. 1 staining programs

+/- buttons - PROGRAM 2 button -

Change displayed Select / program
parameters program no. 2

32 Instruction Manual V 1.4 – 06/2009

 6. Operation

6.5 Switching on mains and selecting direction of travel and alarm volume

• Switch on mains (O = OFF / I = ON).

• The current software version is displayed, in 4 digits, for 10 seconds.

• Only during this phase can the direction of travel and the alarm vol-
ume be selected and/or changed!

• If direction of travel and/or alarm volume have already been selected

and you do not want to make any changes to those parameters either: -
-> go on to Chapter 6.6.

6.5.1 Selecting direction of travel

• Press Start/Stop and hold.

• Depending on the desired direction of travel press the ‘+’ or ‘-’ button:

• Pressing the ‘+’ button:

• Right half of display screen (= indication of seconds) lights up:

--> Direction of travel to the right (= staining starts from the left).

• Pressing the ‘-’ button:

• Left half of display screen (= indication of minutes) lights up:

--> Direction of travel to the left (= staining starts from the right).

Leica ST4040 – Linear Stainer 33

6. Operation

6.5.2 Selecting alarm volume

• Press the ‘Immersion time’ button and hold.

• Press ‘+’ or ‘-’ respectively:

• Each time when pressing a button, the volume changes audibly:
• Alarm OFF --> Alarm low --> Alarm loud.

If ‘Alarm OFF’ is selected (not recommended!), the instrument must be

observed constantly, to make sure all slide racks are removed immedi-
ately when reaching the last station!

• Proceed to --> 6.6.1 ‘Standby mode’.

6.6 Switching on mains without selecting direction of travel/alarm volume

• Switch on mains (O = OFF / I = ON).

• The current software version is displayed for 10 seconds.

• If no further buttons are pressed during this phase, after 10 sec-

onds the LED of the ON/OFF button lights up.

6.6.1 Stand-by mode

• Press ‘ON/OFF’ button.

• The fan goes on.
• The LED (in the program button) of the program used last lights up
(here: ‘Prog 1’) and the programmed parameters are displayed.
• Exception:
Prior to switching off the mains a program had not yet been fini-shed
or a power failure occurred while a program was in progress:
• In those cases the instrument does not switch to the stand-by
mode but continues the previously interrupted program.

34 Instruction Manual V 1.4 – 06/2009

 6. Operation

6.7 Programming

Programming must be done while in stand-by mode. The instrument

can store 2 programs: (buttons ‘Prog 1’ and ‘Prog 2’).

• Select a program (‘Prog 1’ or ‘Prog 2’).

• Press the desired button (here ‘Prog 1’) and hold for approx. 5 seconds
- until the LED in the button starts flashing.

• Press the ‘Immersion time’ button:

• The LED in the button lights up.

• Use the ‘+/-’ buttons to select the desired value.

• Immersion time can be set from 0 seconds up to 99 minutes, 59 sec-

onds.

• To set the time, the button can be pressed and released - going up or
down step by step - or it can be pressed and held. -
If the button is pressed and held, the display scrolls faster.

• Press the ‘Draining time’ button:

• The LED in the button lights up.

• Use the ‘+/-’ buttons to select the desired value.

Leica ST4040 – Linear Stainer 35

6. Operation

• Draining time can be set from 0 seconds to 60 seconds.

• Press the agitation button to activate or deactivate ‘Agitation’.

• (LED illuminated = ‘Agitation’ activated).

• (LED not illuminated = ‘Agitation’ deactivated).

• Briefly press the same program button that was selected when you
startled programming (here ‘Prog 1’), until the LED in the button
lights up.

• The programmed parameters are stored.

• To select and store parameters for program 2, proceed in identical

fashion.

6.8 Staining
6.8.1 Selecting a program

• Select the desired program by pressing button ‘Prog 1’ or ‘Prog 2’.

• Program selection is only possible in stand-by mode.

• LED in the program button lights up

--> Program (here ‘Prog 1’) is selected.

36 Instruction Manual V 1.4 – 06/2009

 6. Operation

6.8.2 Preparing and inserting the slide racks

• Insert the slides into the slide rack/s.

• Attach the slide rack carrier to the slide rack/s.

In addition to using Leica slide racks the Leica ST4040 can also be
run with Medite/Hacker and/or Sakura slide racks. For that pur-
pose, the matching slide rack carriers for those brands have to be
ordered (see chapter 3.4.2 ‘General accessories’).

• Open the loading zone lid.

• Insert the first slide racks into the first stations of the loading zone.
• Insert the slide rack carriers with the attached slide racks into the
center of the stations. Use the notches in the conveyor frame as
orientation help.
• When operating the instrument with optional load / unload sta-tion,
insert the slide rack carriers into the reagent stations of the option-
al load station.

• When staining in two rows, insert the slide racks into the stations of
the loading zone as shown.
• When staining in two rows with optional load / unload station at-
tached to the basic instrument, insert the slide rack carriers into
the reagent stations of the optional load station.

• To stain large slides individually, use the ‘adapter for large slides’.

Leica ST4040 – Linear Stainer 37

6. Operation

6.8.3 Starting the selected program

• With the lid closed, open the ball valve and, if closed as well, open the
water tap.

If not only the ball valve but also the water tap has been closed (-->
e.g. when setting up the instrument), the tap water flow rate has to be
adjusted when starting a program (see also chapter 3.6).

Ball valve closed • For that purpose, press the Start/Stop button:
• The water supply valves are opened.
• The processing mechanism starts.

• Via the ball valve (slowly open or close further) select the appropriate
tap water flow rate (look at the tap water station - see which quantity of
flow looks appropriate).

• The water has to rise inside the tap water station and the water flow
has to be strong enough to wash surplus staining matter from the
specimens.

Ball valve open

However, the flow rate must not be too fast, to ensure that the speci-
mens remain firmly attached to the slide surface!

The ball valve is used to adjust the optimum water flow rate with the
water tap fully open.
If there is no significant water pressure variation in your lab and as
long as the number of tap water stations remains unchanged, the set-
ting of the ball valve should not be altered. To start / end your daily
routine, simply open / close the water tap.

38 Instruction Manual V 1.4 – 06/2009

 6. Operation

6.8.4 Interrupting a program

• If necessary, a staining program can be interrupted pressing the Start/

Stop button.

The Leica ST4040 has a water saving function:

• If a program is interrupted (Start/Stop button),
the water supply to the tap water stations is automatically inter
rupted.
• The remaining water in the tap water stations slowly drains
away through a small opening in the bottom of the station.
• As soon as Start/Stop is pressed again, the program
continues and the water supply to the tap water stations is re
stored.

If a staining program is to be interrupted for an extended period of

time, do not leave any slide racks in the tap water stations, in order
to prevent them from drying out!

• Press Start/Stop again to continue the staining program.

6.9 Removing the slide racks

As soon as the alarm is triggered, immediately remove the completed

slide rack from the last station or from the unload container of the op-
tional unload station! Otherwise, immersion times for the remaining
slide racks will be prolonged!

• The Leica ST4040 is equipped with 2 unload sensors (1 sensor for

each direction of travel), located next to the outer container at each
end of one row.

Leica ST4040 – Linear Stainer 39

6. Operation

• When the alarm is triggered, immediately open the unloading zone lid
and take out the slide rack.
• As soon as the slide rack has been removed, the program continues.
• Close the unloading zone lid.

6.9.1 Removing the last slide rack

• First remove the slide rack.

• Then, with the conveyor frame in the lowest position, press Start/Stop.
• This ends the program and shuts off the water valves.

6.10 Removing the slide racks in instruments equipped with optional unload station

• To remove the slide racks, proceed as described above (‘alarm is trig-

gered - remove the racks’).
• Instruments equipped with optional unload station allow considerably
more time before the slide racks have to be removed.
The unload station can hold 6 slide racks, i.e., immediate rack removal
is necessary only with every 6th rack.

As long as a slide rack carrier is located on the red ramps, the instru-
ment will not resume processing, i.e. should you decide to remove
only some of the completed slide racks from the optional unload sta-
tion, at any rate make sure to clear the red ramps!

Outside the instrument, the unload container with the corresponding

lid (see picture left) can be used for intermediate storage of slide
rack carriers prior to cover-slipping (see accessory list in chapter
4.3.2).

40 Instruction Manual V 1.4 – 06/2009

 6. Operation

6.11 Finishing work

• Once the last slide rack is removed, press ‘Start/Stop’.

• Close the ball valve.

Do not change the setting of the water tap, so that the adjusted tap
water flow rate remains unaltered!

• If necessary, add or exchange reagents.

• Cover the stations with the station lids.

• Close the lids.
• Leave the fan on, i.e.:
• Do not switch off the instrument via the ‘ON/OFF’ button or via the
mains switch.

Leica ST4040 – Linear Stainer 41

7. Trouble Shooting

Problem Possible cause What to do

No tap water supply. - Water tap closed. - Open water tap.

- Ball valve closed. - Open ball valve.

- Magnetic valve or magnetic - Call Technical Service.

valve drive defective.

- In-house plumbing - Call in-house technical ser-

problem (pipes clogged / vice / plumber.
furred).

Slide racks do not advance - Slide racks / slide rack car- - If bent only slightly, read-
and/or are not lowered into re- riers bent out of shape. just carefully.
agent / tap water stations.
- Slide rack carriers / slide - Reinsert correctly.
racks jammed.

- Slide racks and/or slide - Parts not usable any more -

rack carriers significantly -> discard.
bent out of shape or weld-
ing spots broken.

- Toothed belt torn. - Call Technical Service.

- Motor or motor drive defec- - Call Technical Service.

tive.

Waste water does not drain - No or only insufficient gra- - Install drain hose so there
away. dient of drain hose. is a sufficient gradient from
drain outlet on instrument
to drain pipe.

- Drain of trough in basic in- - Remove station and clean

strument clogged up. drain with fine bottle brush
or similar.

42 Instruction Manual V1.4 – 06/2009

 7. Trouble Shooting

Problem Possible cause What to do

Waste water does not drain - Drain hose clogged up - Clean drain hose (see
away. (algae). chapter 8 - ‘Cleaning /Main-
tenance’) - If necessary, re-
place drain hose by new
one.

Fan does not work. - Fan or fan drive defective. - Call Technical Service.

Controls do not work - PCB defective. - Call Technical Service.

(buttons do not respond, no
display indication). - Contacts loose. - Call Technical Service.

- Control panel drive defec- - Call Technical Service.

tive.

Leica ST4040 – Linear Stainer 43

8. Cleaning and Maintenance

8.1 Cleaning

Prior to cleaning the instrument, always switch off mains and unplug
the power chord!

Dispose of used reagents according to the laboratory regulations in

force in your country!

Spilled solvents (reagents) have to be wiped away immediately! - In

case of long-term exposure, the lid surfaces are only conditionally
resistant to solvents!

The painted surfaces and the control panel are not resis-tant to xy-
lene or acetone!

For cleaning the instrument, do not use any one of the following: al-
cohol, detergents containing alcohol (window cleaner!), abrasive
cleaning powders, solvents containing xylene or acetone!

To clean the lids, control panel and housing, use mild household de-
tergents - see safety instruction above for non-appropriate ingredi-
ents.

When handling cleaning detergents, follow the instructions of the

manufacturer and make sure all applicable laboratory regulations
are complied with!

When cleaning the instrument, no liquid may enter in contact with

any of the electrical connections or get into the interior of the instru-
ment!

44 Instruction Manual V 1.4 – 06/2009

 8. Cleaning and Maintenance

8.1 Cleaning

• Clean the reagent and tap water stations as well as the trough which
holds the reagent and tape water stations regularly.

• For that purpose, remove the reagent and tap water stations from the
trough.

• The reagent and tap water stations can be washed in a laboratory

dishwasher.

Wash the tap water and reagent stations in the dishwasher at a tem-
perature of max. +65 °C . - Use a standard detergent for laboratory
dishwashers.
At any rate avoid washing the stations at higher temperatures (e. g.
in industrial diswashers which run at a temperature of +85 °C), as the
stations may become deformed!

• To clean the painted instrument surfaces and the lids, use a mild
household detergent (see safety instructions on previous page for non-
appropriate ingredients).

• From time to time check the drain hose for accumulated dirt, especially
algae - clean if necessary.

Leica ST4040 – Linear Stainer 45

8. Cleaning and Maintenance

8.2 Maintenance

Only authorized Leica technical service engineers may open the in-
strument for maintenance and repair work.
Exception: Changing the activated carbon filter - this is the only
maintenance task to be carried out by the user!
For your own safety, never try to carry out any repairs yourself!

Any unauthorized repair, whether carried out by the user or any third
party not authorized by Leica will void the guarantee (see also Chap-
ter 9.1 ‘Warranty’).

The Leica ST4040 linear stainer is virtually maintenance-free in operation.

However, to ensure a smooth operation of the instrument over many
years, we recommend the following:
• Have the instrument inspected once per year by a qualified service en-
gineer authorized by Leica.
• Enter into a service contract at the end of the warranty period. For
more information, please contact your local Leica technical service
center.
• Exchange the activated carbon filter regularly and dispose of it ac-
cording to the applicable laboratory rules in your country.

46 Instruction Manual V 1.4 – 06/2009

 9. Warranty and Service

Warranty
Leica Biosystems Nussloch GmbH guarantees that the contractual prod-
uct delivered has been subjected to a comprehensive quality control pro-
cedure based on the Leica in-house testing standards, and that the prod-
uct is faultless and complies with all technical specifications and/or
agreed characteristics warranted.
The scope of the warranty is based on the content of the concluded
agreement. The warranty terms of your Leica sales organization or the or-
ganization from which you have purchased the contractual product shall
apply exclusively.

Service information
If you require technical service or replacement parts, please contact your
Leica sales representative or dealer who sold the product.
Please provide the following information:
• Model name and serial number of the instrument.
• Location of the instrument and name of the person to contact.
• Reason for the service call.
• Date of delivery.

Decommissioning and disposal

The instrument or parts of the instrument must be disposed of in compli-
ance with the local laws.

Leica ST4040 – Linear Stainer 47

10. EC Declaration of Conformity

EC Declaration of Conformity
We herewith declare, in exclusive responsibility, that the

Leica ST4040 – Linear stainer

was developed, designed and manufactured to conform with the
• Directive 2006/95/EC of the European Parliament and of the Council (Low Voltage)
• Directive 2004/108/EC of the European Parliament and of the Council (electromagnetic compatibility)
• Directive 98/79/EC of the European Parliament and of the Council (in-vitro diagnostic medical devices)

The following harmonized standards were applied:

• EN 61010-1: 2001
Safety requirements for electrical equipment for measurement, control and laboratory use
Part 1: General requirements
• EN 61326: 2006
Electrical equipment for measurement, control and laboratory use -
EMC requirements -
Part 1: General requirements
• DIN EN 61010-2-101: 2002
Safety requirement for electrical equipment for measurement, control and laboratory use
Part 2-101: Particular requirements for in vitro diagnostic (IVD)
• EN 14971: 2007
Medical devices - Application of risk management to medical devices
• EN 591: 2001
Instruction for use for in vitro diagnostic instruments for professional use

In addition, the following in-house standards were applied:

• DIN EN ISO 9001: 2000.
Quality management systems - Requirements

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17-19 ..................................
D-69222 Nussloch Anne De Greef-Safft
May 15, 2008 President Biosystems Devision

48 Instruction Manual V 1.4 – 06/2009

 Appendix 1

Staining Protocols for Leica ST4040 Linear Stainer

Station no. Elastica van Gieson H&E

1 Xylene Xylene
2 Xylene Xylene
3 Xylene Xylene
4 100 % Alcohol Xylene
5 96 % Alcohol 100 % Alcohol
6 75 % Alcohol 100 % Alcohol
7 Resorcinol G 96 % Alcohol
8 Resorcinol G 75 % Alcohol
9 Running water Running water
10 Running water Distilled water
11 Distilled water Haemalum
12 Weigert iron haemalum Haemalum
13 Weigert iron haemalum Running water
14 25 % HCl in 96 % alcohol Running water
15 Running water 25 % HCl in water
16 Running water Running water
17 Distilled water 75 % Alcohol
18 van Gieson Eosine
19 van Gieson Eosine
20 96 % Alcohol 96 % Alcohol
21 96 % Alcohol 96 % Alcohol
22 100 % Alcohol 100 % Alcohol
23 100 % Alcohol 100 % Alcohol
24 100 % Alcohol 100 % Alcohol
25 Xylene Xylene
26 Xylene Xylene
27 Xylene Xylene

Immersion time: 1 Minute

Draining time: 5 Seconds
Agitation: ON

Leica ST4040 – Linear Stainer 49

Appendix 2

Ordering Information

Lid, cpl. w/handle 14 0474 32255

Slide rack carrier Leica 14 0474 32305
Slide rack carrier Medite 14 0474 32258
Slide rack carrier Sakura 14 0474 32296
Slide rack cpl. 14 0474 32789
Sakura slide rack 14 0474 33463
CV5000 adapter, stationary 14 0474 32793
CV5000 adapter, removable 14 0474 32794
Storage container 14 0474 32261
Activated carbon filter 14 0474 32273
Suction tube 2m 14 0422 31974
Suction tube 4m 14 0422 31975
Tap water station, assy. 14 0474 32256
Reagent station 14 0474 32271
Optional load station right 14 0474 32241
Optional load station left 14 0474 32242
Optional unload station right 14 0474 32243
Optional unload station left 14 0474 32244
Lid for reagent stations, opt . load station 14 0474 33092
Lid for unload container 14 0474 33093
Unload container 14 0474 32363
Tap water inlet hose 14 0474 32325
Drain hose, 4 m 14 0474 33147
Adapter for large slides, 50 x 75 mm 14 0456 27069
Disposable plastic film, for control panel 14 0474 33176
Power cord Australia 14 0411 32565
Power cord 'EU' 14 0411 13558
Power cord 'USA-CAN-J' 14 0411 13559
Power cord 'UK' 14 0411 27822
Slow-blowing fuse 6.3x32 T8.0A 14 6943 08001
Slow-blowing fuse 6.3x32 T0.6A 14 6943 00601
Slow-blowing fuse T1.6A 14 6943 01601

50 Instruction Manual V 1.4 – 06/2009