MW-12A

Microplate Washer

Operator’s Manual
 © 2007-2010 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. All rights
Reserved.

For this Operator’s Manual, the issue date is 2010-04.

Intellectual Property Statement

SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter
called Mindray) owns the intellectual property rights to this Mindray product and this
manual. This manual may refer to information protected by copyright or patents and
does not convey any license under the patent rights or copyright of Mindray, or of
others.

Mindray intends to maintain the contents of this manual as confidential information.

Disclosure of the information in this manual in any manner whatsoever without the
written permission of Mindray is strictly forbidden.

Release, amendment, reproduction, distribution, rental, adaptation, translation or

any other derivative work of this manual in any manner whatsoever without the
written permission of Mindray is strictly forbidden.

, , , , BeneView,
WATO, BeneHeart, are the trademarks, registered or otherwise, of Mindray in
China and other countries. All other trademarks that appear in this manual are
used only for informational or editorial purposes. They are the property of their
respective owners.

Responsibility on the Manufacturer Party

Contents of this manual are subject to change without prior notice.

All information contained in this manual is believed to be correct. Mindray shall not
be liable for errors contained herein or for incidental or consequential damages in
connection with the furnishing, performance, or use of this manual.

Mindray is responsible for the effects on safety, reliability and performance of this
product, only if:

„ all installation operations, expansions, changes, modifications and repairs of

this product are conducted by Mindray authorized personnel;

„ the electrical installation of the relevant room complies with the applicable
national and local requirements; and

„ the product is used in accordance with the instructions for use.

i
 WARNING:
is important for the hospital or organization that employs this equipment
to carry out a reasonable service/maintenance plan. Neglect of this
may result in machine breakdown or personal injury.

NOTE:
his equipment must be operated by skilled/trained clinical professionals.

Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,
EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY
OR FITNESS FOR ANY PARTICULAR PURPOSE.

Exemptions
Mindray's obligation or liability under this warranty does not include any
transportation or other charges or liability for direct, indirect or consequential
damages or delay resulting from the improper use or application of the product or the
use of parts or accessories not approved by Mindray or repairs by people other than
Mindray authorized personnel.

This warranty shall not extend to:

„ Malfunction or damage caused by improper use or man-made failure.

„ Malfunction or damage caused by unstable or out-of-range power input.
„ Malfunction or damage caused by force majeure such as fire and earthquake.
„ Malfunction or damage caused by improper operation or repair by unqualified or
„ unauthorized service people.
„ Malfunction of the instrument or part whose serial number is not legible enough.
„ Others not caused by instrument or part itself.

Company Contact

Manufacturer: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.

E-mail Address: [email protected]
Tel: +86 755 26582479 26582888
Fax: +86 755 26582934 26582500

EC-Representative: Shanghai International Holding Corp. GmbH(Europe)

Address: Eiffestraβe 80, Hamburg 20537, Germany
Tel: 0049-40-2513175
Fax: 0049-40-255726

ii
 Preface

Before using the MW-12A, please read this operator’s manual thoroughly for
relevant operation instructions.

Intended Audience
This manual is geared for clinical professionals to:

„ Operate the MW-12A;

„ Maintain and troubleshoot the MW-12A;
„ Learn about the MW-12A operation.

WARNING
The MW-12A Microplate Washer is to be operated only by clinical
professionals, doctors or laboratory experimenters that are trained
by Mindray or Mindray-authorized distributors.

What Can You Find in This Manual

This manual covers principles, basic operating instructions, maintenance and
troubleshooting guidelines of the MW-12A. Please operate and troubleshoot the
system strictly as instructed by this manual.

Preface 1
Conventions

Safety Symbols
Safety symbols alert you to potentially dangerous conditions and information
that requires your attention. These safety symbols, together with related text,
apply to specific procedures and appear as needed throughout this manual.

When you see… Then…

WARNING Read the statement following the symbol. The
statement is alerting you to an operating
hazard that can cause personal injury.
Read the statement following the symbol. The
BIOHAZARD
statement is alerting you to a potentially
biohazardous condition.

CAUTION Read the statement following the symbol. The

statement is alerting you to a possibility of
system damage or unreliable results.
Read the statement following the symbol. The
NOTE
statement is alerting you to information that
requires your attention.

Labels
The labels attached to the surface of the instrument use symbols to clarify the
meaning of the text. If any of the labels peels off, contact Mindray customer
service department or your local distributor for replacement. The following chart
explains the labels used in this manual.

Serial number

Manufacture date

Manufacturer

The device is fully in conformance with the Council

Directive Concerning In Vitro Diagnostic Medical Devices
98/79/EC.

Authorized Representative in the European Community

The following definition of the WEEE label applies to EU

member states only: The use of this symbol indicates that
this product should not be treated as household waste. By
ensuring that this product is disposed of correctly, you will
help prevent bringing potential negative consequences to
the environment and human health. For more detailed
information with regard to returning and recycling this
product, please consult the distributor from whom you
purchased the product.

2 Preface
 In Vitro diagnostic equipment

BIOHAZARD: risk of potentially biohazardous infection

WARNING: risk of personal injury or equipment damage

100-240V～ Alternating current (AC) 100～240V

50/60Hz 100VA Frequency 50/60Hz , Power 100VA

Protect ground terminal

Power ON

Power OFF

Communication interface for PC

Wash1 Wash1 inlet or wash1 bottle
Wash2 Wash2 inlet or wash2 bottle
Distilled Water Distilled water bottle
Waste Waste outlet or waste bottle
Buffer Buffer inlet or buffer bottle
Sensor Waste sensor port

Graphics
All graphics, including screens, are for illustration purpose only and must not be
used for any other purposes.

EC Representative
Name: Shanghai International Holding Corp. GmbH (Europe)
Address: Eiffestraβe 80, 20537 Hamburg Germany
Phone: 0049-40-2513175
Fax: 0049-40-255726

Preface 3
Safety Precautions
Observe theses safety precautions when using the MW-12A Microplate Washer.
Ignoring any of the precautions may lead to personal injury or equipment
damage.

WARNING
If the instrument is used in a manner not specified by Mindray, the
protection provided by the system may be impaired.

Preventing Electric Shock

Please observe the following instructions to prevent electric shock.

WARNING
When the instrument is powered on, users must not remove its
enclosure.
Spillage of liquid on the analyzer may cause equipment failure and
even electric shock. In case of spillage, switch off the power
immediately, remove the spillage and contact Mindray’s customer
service department or your local distributor.

Preventing Personal Injury Caused by Moving Parts

Please observe the following instructions to prevent personal injury caused by

moving parts.

WARNING
Do not touch such moving parts as plate carrier, manifold, etc.
when the system is in operation.
Do not put your finger or hand into any open part when the system
is in operation.

Preventing Infection

Please observe the following instructions to protect against the biohazardous

infection.

BIOHAZARD
Inappropriately handling samples may lead to biohazardous
infection. Do not touch the sample or waste with your hands. Wear
gloves and lab coat and, if necessary, goggles.
In case your skin contacts the sample or waste, follow standard
laboratory safety procedures and consult a doctor.

4 Preface
Treating Waste Liquids

Please observe the following instructions to prevent environmental pollution and

personal injury caused by waste.

BIOHAZARD
Some substances in reagent, sample, wash solution and waste are
subject to regulations of contamination and disposal. Dispose of
them in accordance with local or federal guidelines for
biohazardous waste disposal and consult the manufacturer or
distributor of the reagents for details.

Treating Waste Parts

Please observe the following instruction to dispose of the used parts to prevent
from environmental pollution and personal injury.

BIOHAZARD
Materials of the manifold, plastic bottle, tubing or washer are
subject to contamination regulations. Dispose of them in
accordance with local or federal guide lines for biohazardous waste
disposal.

Preventing Fire or Explosion

Please observe the following instructions to prevent fire and explosion.

WARNING
Ethanol is flammable substance. Please exercise caution while
using the ethanol. Do not use flammable substance around this
instrument.

Preface 5
Precautions on Use
To use the MW-12A Microplate Washer safely and efficiently, please pay much
attention to the following operation notes.

Intended Use

WARNING
This instrument is intended for ELISA test in a clinical laboratory
and used in conjunction with Microplate Reader.
Please consult Mindray first if you want to use the system for other
purposes.

Operator

WARNING
The MW-12A Microplate Washer is to be operated only by clinical
professionals, doctors or laboratory experimenters that are trained
by Mindray or Mindray-authorized distributors.

Environment

CAUTION
Please install and operate the system in an environment specified
by this manual. Installing and operating the system in other
environment may lead to poor washing effect and even equipment
damage.
To relocate the system, please contact Mindray customer service
department or your local distributor.

6 Preface
Preventing Interference by Electromagnetic Noise

CAUTION
Do not use this device in close proximity to sources of strong
electromagnetic radiation (e.g. unshielded intentional RF sources),
as these may interfere with the proper operation. The
electromagnetic environment should be evaluated prior to
operation of the device. This equipment has been designed and
tested to CISPR 11 Class A, in a domestic environment it may
cause radio interference, in which case, you may need to take
measures to mitigate the interference. Do not install devices
generating excessive electromagnetic noise around the system. Do
not use such devices as mobile phones or radio transmitters in the
room housing the system. Do not use other CRT displays around
the system. Interference by electromagnetic noise may lead to
system failure.
Do not use other medical instruments around the system that may
generate electromagnetic noise to interfere with their operations.

Communication Port

WARNING
This instrument has a serial port that is used only for upgrading the
system software. Users must not operate the instrument via this
serial port; otherwise equipment damage may be caused.

Preface 7
8 Preface
 Contents

Intellectual Property Statement ....................................................................................... i

Responsibility on the Manufacturer Party ....................................................................... i
Warranty ........................................................................................................................ ii

Preface............................................................................................................... 1
Intended Audience .........................................................................................................1
What Can You Find in This Manual................................................................................1
Conventions...................................................................................................................2
Safety Precautions.........................................................................................................4
Precautions on Use .......................................................................................................6

1 The Basics ...............................................................................................1-1

1.1 System Description ......................................................................................1-3
1.2 Appearance ..................................................................................................1-3
1.3 Control Mode................................................................................................1-4
1.4 Operation Tips ..............................................................................................1-4
1.5 Storage .........................................................................................................1-4

2 Installation................................................................................................2-1
2.1 Unpacking ....................................................................................................2-3
2.2 Installation Requirements .............................................................................2-3
2.2.1 Space Requirements ......................................................................2-3
2.2.2 Power and Environmental Requirements .......................................2-3
2.3 Installing the Washer ....................................................................................2-4
2.4 Installing the Manifold...................................................................................2-4
2.5 Connecting the Bottles .................................................................................2-5
2.6 Installing the Power Cord .............................................................................2-6

Contents I
3 Basic Operations .................................................................................... 3-1
3.1 Overview ......................................................................................................3-3
3.1.1 Keypad...........................................................................................3-3
3.1.2 Main Screen...................................................................................3-3
3.2 Needle Positions ..........................................................................................3-4
3.2.1 Manifold Selection..........................................................................3-4
3.2.2 Position Adjustment .......................................................................3-5
3.3 Parameters................................................................................................. 3-11
3.3.1 Parameter Configuration Screen.................................................. 3-11
3.3.2 Setting up Programs ....................................................................3-12
3.4 Running a Program ....................................................................................3-16
3.5 Maintenance...............................................................................................3-19
3.6 Waste Full ..................................................................................................3-20

4 Maintenance ............................................................................................ 4-1

4.1 Daily Maintenance........................................................................................4-3
4.2 Weekly Maintenance ....................................................................................4-3
4.2.1 Maintaining the Solenoid Valve Tubing ..........................................4-3
4.2.2 Maintaining the manifold connector ...............................................4-4
4.3 Monthly Maintenance ...................................................................................4-5
4.4 As-Needed Maintenance..............................................................................4-5
4.4.1 Cleaning Fluidic System ................................................................4-5
4.4.2 Cleaning Mechanical Parts ............................................................4-5

5 Troubleshooting...................................................................................... 5-1
5.1 Manifold Failure............................................................................................5-3
5.2 Movement Error ...........................................................................................5-4
5.3 Dispense Needle Error .................................................................................5-4
5.4 Residue Too Much........................................................................................5-5
5.5 Wash Solution Overflow ...............................................................................5-6
5.6 Collision of Manifold and Microplate.............................................................5-6

6 Appendix ................................................................................................. 6-1

Appendix A Specifications .................................................................................6-3
Appendix B Supplies .........................................................................................6-4
Appendix C Index ..............................................................................................6-5

II Contents
 1 The Basics
1.1 System Description ......................................................................................1-3
1.2 Appearance ..................................................................................................1-3
1.3 Control Mode................................................................................................1-4
1.4 Operation Tips ..............................................................................................1-4
1.5 Storage .........................................................................................................1-4

The Basics 1-1

1-2 The Basics
 1 The Basics

1.1 System Description

Components: This instrument is supplied with the washer, wash bottles, waste
bottle, distilled water bottle and buffer bottle.

Principles: This instrument consists of hardware, software, mechanics and

fluidic part. Controlled by the software program, the instrument
can automatically dispense wash solution into microplate wells
and discharge the waste fluid to waste bottle. The manifold and
plate carrier can move vertically and horizontally to aspirate and
dispense liquid.

1.2 Appearance
The MW-12A Microplate Washer applies to 96-/48-well microplates. It is
supplied with a 12-/8-channel manifold, wash bottles, waste bottle, and buffer
bottle, and supports two types of wash solution.

The Basics 1-3

1.3 Control Mode
The MW-12A Microplate Washer can be operated via the keypad on the front
panel.

1.4 Operation Tips

The power indicator is located on the lower right corner of the LCD. It lights:

„ ON when the system is powered on.

„ OFF when the system can not work normally.

When powered on, the washer performs an initialization, which includes

mechanical reset, waste check, manifold position check, etc, and then displays
the main screen.

NOTE
The initialization will take about 20 seconds.

Fore detailed operations of the instrument, refer to 3 Basic Operations.

You can follow this procedure to operate the instrument:

1 Fill each wash bottle with appropriate wash solution, then connect the
bottles to the washer.
2 Fill the distilled water bottle with distilled water or deionized water for use
in washing.
3 Connect the buffer bottle and empty waste bottle to the washer.
4 Power on the washer. When startup initialization is finished, configure the
wash positions, parameters and then run the wash programs.
5 When the wash procedure is finished, connect the distilled water bottle to
Wash2 port on rear panel of the washer, then launch a rinse
maintenance procedure to clean the tubing for no less than 15s.
6 Power off the washer.
7 Empty and clean the waste bottle in accordance with your laboratory
safety regulations.

CAUTION
When the instrument is not used, lay down the aerosol cover to
prevent from dust and foreign matters.

1.5 Storage
The washer and packaging materials should be stored in an environment that
meets the following requirements:

1-4 The Basics

Storage temperature: 0°C-40°C

Relative humidity: 15%-85%

Free of erosive gas and well ventilated

CAUTION
The washer should be stored without any residual fluid in it.
Perform the following steps before storing the washer:
1) Before turning off the washer, run a rinse procedure for less
than 20s with distilled water or deionized water.
2) When rinsing is finished, disconnect the two wash bottles from
the washer, and place a used microplate on the plate carrier.
3) On the parameter configuration screen, select Wash1 and run
at least one wash program, then launch a rinse procedure for
over 15 seconds.
4) Turn off the washer.

The Basics 1-5

1-6 The Basics
 2 Installation
2.1 Unpacking ....................................................................................................2-3
2.2 Installation Requirements .............................................................................2-3
2.2.1 Space Requirements ......................................................................2-3
2.2.2 Power and Environmental Requirements .......................................2-3
2.3 Installing the Washer ....................................................................................2-4
2.4 Installing the Manifold...................................................................................2-4
2.5 Connecting the Bottles .................................................................................2-5
2.6 Installing the Power Cord .............................................................................2-6

Installation 2-1
2-2 Installation
 2 Installation

WARNING:
The MW-12A should be installed only by Mindray or
Mindray-authorized technicians. You should prepare a place
meeting the requirements specified in 2.2 Installation
Requirements

2.1 Unpacking
When you receive the instrument, carefully inspect the package. If you see any
signs of mishandling or damage, file a claim immediately with Mindray
Customer Service Department or your local distributor.

After opening the package, check the delivered goods against the packing list
as well as the appearance of the instrument. If you find anything missing or
damaged, alert Mindray Customer Service Department or your local distributor
immediately.

2.2 Installation Requirements

CAUTION:
Make sure the system is installed in a place meeting the
requirements specified in this manual. Otherwise, it will not perform
as promised.

2.2.1 Space Requirements

„ The instrument is for indoor use only.
„ The operating platform should be level (gradient less than ∠1:200), stable,
dry and tidy.
„ Enough space should be ensured for connection of lines and bottles. You’re
recommended to set the instrument on a platform that has a length of no
less than 60cm and width of no less than 70cm.

2.2.2 Power and Environmental Requirements

„ Ambient temperature: 5°C-35°C
„ Relative humidity: 15%-85%
„ Power supply: 100-240V~, 50/60Hz
„ Power consumption: 100VA

Installation 2-3
 „ Atmospheric pressure: 70 kPa-106kPa
„ The instrument should be far from strong magnetic fields.
„ The instrument should be shielded from direct sunlight.
„ The instrument should be properly grounded.
„ The installation site should be well ventilated.

2.3 Installing the Washer

Follow this procedure to install the washer:

1 After opening the package, take out the fitting pack and washer. Open
the fitting pack and take out the wash bottles, tubing, etc.
2 Set the washer on the operating platform and keep it at least 15cm away
from the wall. Remove the packaging material from the washer.
3 Remove the protective materials from the manifold support, plate carrier
and tubing.
4 Install the manifold as per section 2.4 Installing the Manifold.
5 Install the bottles and waste sensor as per section 2.5 Connecting the
Bottles and 2.6 Installing the Power Cord.

2.4 Installing the Manifold

1 Open the aerosol cover and place the manifold on the manifold support
horizontally, allowing the two retaining screws on the manifold to lie in the
support grooves.

2-4 Installation
 2 One end of the dispensing tubing (thinner) and waste tubing (thicker) has
been connected to corresponding black and red ports on the washer.
Connect the other end of the dispensing tubing and waste tubing
respectively to the smaller and bigger connectors on the manifold.

NOTE
Be sure to insert the tubing into the connector until the tubing
cannot proceed any more.

2.5 Connecting the Bottles

The MW-12A Microplate Washer is provided with the wash1 bottle, wash2 bottle,
buffer bottle, waste bottle and distilled water bottle. The distilled water bottle can
be leave aside at the beginning and connected to the washer only when wash2
bottle is empty during rinse process. The following figure shows all connectors
on the rear panel of the washer.

Installation 2-5
 1 Connect one end of wash1 tubing (black) to Wash1 connector on the
washer and the other end to the black fitting on wash1 bottle.

2 Connect one end of wash2 tubing (green) to Wash2 connector on the

washer and the other end to the green fitting on wash2 bottle.
3 Connect one end of waste tubing (red) to Waste connector on the washer
and the other end to the red fitting on waste bottle.
4 Connect one end of pump tubing (blue) to Buffer connector on the
washer and the other end to the blue fitting on buffer bottle.
5 Use the buffer tubing to connect the waste bottle and buffer bottle.
6 The waste sensor is fixed on the waste bottle. Connect the waste sensor
line to Waste Sensor connector on the rear panel of the washer.

NOTE
Be sure to insert the tubing into the connector until the tubing
cannot proceed any more.
Be sure to tighten the caps of waste bottle and buffer bottles during
washing process. Otherwise good aspirating may not be achieved.

2.6 Installing the Power Cord

Connect one end of the power cord to the power socket on rear panel of the
washer and other end to the power supply. Switch on the power. The instrument
is started and starts initializing. The initialization process includes mechanical
reset, waste detection and manifold check, and lasts for about 20s.

2-6 Installation
WARNING
The instrument uses a three-wire power cord and should be
grounded properly; otherwise electric shock or equipment damage
may be caused.
A fuse ([email protected], slow-blow) is provided on the instrument
and must not be replaced by user.
Ensure that the power supply complies with the power
requirements specified in this manual and an appropriate fuse is
installed.
CAUTION
Ensure that the instrument is switched off before connecting the
power cord.

Installation 2-7
2-8 Installation
 3 Basic Operations
3.1 Overview ......................................................................................................3-3
3.1.1 Keypad ...........................................................................................3-3
3.1.2 Main Screen ...................................................................................3-3
3.2 Needle Positions ..........................................................................................3-4
3.2.1 Manifold Selection..........................................................................3-4
3.2.2 Position Adjustment........................................................................3-5
3.3 Parameters................................................................................................. 3-11
3.3.1 Parameter Configuration Screen.................................................. 3-11
3.3.2 Setting up Programs ....................................................................3-12
3.4 Running a Program ....................................................................................3-16
3.5 Maintenance...............................................................................................3-19
3.6 Waste Full ..................................................................................................3-20

Basic Operations 3-1

3-2 Basic Operations
 3 Basic Operations

3.1 Overview
The MW-12A Microplate Washer can be operated via the keypad on the front
panel.

3.1.1 Keypad
The keypad of the instrument is shown in the figure below.

WASH PARA POS MAINT OK EXIT

WASH Press to start a wash program.

PARA Press to set up wash programs.
POS Press to adjust the dispensing/aspiration positions of the
needles.
MAINT Press to rinse the tubing.
OK Press to confirm your settings and proceed to the next
parameter.
EXIT Press to exit and return to the previous menu with your settings
saved.
key Press to increase the value of selected parameter or toggle
between options.
key Press to decrease the value of selected parameter or toggle
between options.

3.1.2 Main Screen

Switch on the power supply. The system performs an initialization and then
displays the main screen as shown below.

Parameter Position
Wash Maintenance

Basic Operations 3-3

3.2 Needle Positions
Press the POS key on the front panel of the washer. The needle position setup
screen is displayed as follows.

12 Channel
Cent Pos Left Pos
Right Pos Disp Pos
Bot Auto Ht Bot Manu Ht

On the needle position setup screen:

„ X Channel: It indicates manifold type and includes 12- and 8- channel.

„ XX(value): It indicates the position parameter(mm) you’re configuring and is
displayed on the upper-right corner of the screen.
„ Cent Pos: Select to adjust the position of the aspirate needle to the center of
the well. This central position is the default aspirate position for U-/V-bottom
plates.
„ Left Pos: Select to adjust the first aspirate position for crosswise aspiration.
„ Right Pos: Select to adjust the second aspirate position for crosswise
aspiration or to adjust the aspirate position for standard aspiration.
„ Disp Pos: adjust the dispensing position of the dispense needle or the
overflow height.
„ Bot Auto Ht: detect the well bottom and determines the aspiration height.
„ Bot Manu Ht: allow the user to define the bottom aspiration height.

3.2.1 Manifold Selection

An appropriate manifold should be selected according to the microplate and its
placement on the carrier. When changing the manifold, you must reset the
manifold type and other parameters on the needle position setup screen.

NOTE
When using 48-well plates, you must select 12-channel manifold
and fix it with the positioning plate provided with the instrument.
When changing the plate type, you must reset the parameters on
the needle position setup screen.

1 Press the POS key on the front panel of the washer. The needle position
setup screen shows and the manifold type is highlighted by default

12 Channel
Cent Pos Left Pos
Right Pos Disp Pos
Bot Auto Ht Bot manu Ht

2 Select 12-Channel or 8-Channel by pressing or .

3-4 Basic Operations

 3 Press OK to save your selection and proceed to the next parameter
(Cent Pos).
4 Otherwise, press EXIT to save your settings and return to the main
screen.

Parameter Position
Wash Maintenance

3.2.2 Position Adjustment

NOTE
The washer has been aligned with standard microplate before
leaving the factory. However, you need to adjust the needle
position according to your plate type, so that the washer can
operate more effectively.
Different microplates may be different in height. Before using the
plates, be sure to set them level and make sure no microwell is
protruded.

Aspiration height refers to the depth that the aspirate needle moves down to the
well during washing. During wash cycles, the aspirate needle aspirates liquid
from the well and sucks it into the waste bottle. You must set appropriate left
and right positions based on different plate types.

Dispense needle Aspirate needle Dispense needle Aspirate needle

Home
position Home
position

Left Pos
Dispensing
height
(reference)

Aspiration
Right Pos
height
(reference)

Microwell
Asp left
Asp tight
position
position
Central position(reference) Central position(reference)

Basic Operations 3-5

 1 After selecting a manifold, press OK. Cent Pos is highlighted. The
needles move to the top of the first row of the plate.

12 Channel XX
Cent Pos Left Pos
Right Pos Disp Pos
Bot Auto Ht Bot Manu Ht

2 Move the plate carrier rightwards or leftwards by selecting or

until the needles lie in the center of the wells. (Select to move right
and to move left.) The position value is displayed on the upper
right corner of the screen.
3 Press OK to save your settings and highlight Left Pos. The needles
move to the top left of the first row of the plate.

12 Channel XX
Cent Pos Left Pos
Right Pos Disp Pos
Bot Auto Ht Bot Manu Ht

4 Move the plate carrier rightwards or leftwards by selecting or

until the aspirate needles reach the aspiration left position. The position
value is displayed on the upper right corner of the screen. (Select
to move right and to move left.)
Dispense needle Aspirate needle

Home
position

Left Pos
Dispensing
height
(reference)

Right Pos

Microwell

Central position(reference)

3-6 Basic Operations

5 Press OK to save your settings and highlight Right Pos. The needles
move to the top right of the first row of the plate.

12 Channel XX
Cent Pos Left Pos
Right Pos Disp Pos
Bot Auto Ht Bot Manu Ht

6 Move the plate carrier rightwards or leftwards by selecting or

until the aspirate needles reach the aspiration right position. The
position value is displayed on the upper right corner of the screen.
(Select to move right and to move left.)
Dispense needle Aspirate needle

Home
position

Left Pos
Dispensing
height
(reference)

Right Pos

Microwell

Central position(reference)

NOTE
The left and right positions, relating to crosswise washing,
must not go beyond the microwell to avoid damaging the
needles. Also the two positions must not be too close to
the well center to achieve best washing effects. It is
recommended that the two positions set to about
0.2-0.5mm away respectively from the left and right walls
of the well.

Basic Operations 3-7

 7 Press OK to save your settings and highlight Disp Pos. The needles
move to the center of the first row of the plate.

12 Channel XX
Cent Pos Left Pos
Right Pos Disp Pos
Bot Auto Ht Bot Manu Ht

NOTE
The dispensing position must not be set too high, so that
the aspirate needles are far from the well and overflow is
caused. Also the dispensing position must not be set too
low, so that the aspirate needles move deep into the well
and the well opening cannot be washed thoroughly. You
are recommended to adjust the aspirate needles to be
about ±0.5mm away from the well.

8 Move the manifold upwards or downwards by selecting or

until the dispense needles reach the dispensing position. The position
value is displayed on the upper right corner of the screen.
Dispense needle Aspirate needle

Home
position

Left position

Dispensing
height
(reference)
Right position

Adjustment
range
(reference)

Microwell

Central position(reference)

9 Press OK to save your settings and highlight Bot Auto Ht. The needles
move to the bottom of the first row of the plate.

12 Channel XX
Cent Pos Left Pos
Right Pos Disp Pos
Bot Auto Ht Bot Manu Ht

3-8 Basic Operations

10 The washer detects the well bottom automatically and determines the
aspiration height. The value is displayed on the upper right corner of the
screen.
Dispense needle Aspirate needle

Home
position

Aspiration
height
(reference)

Asp left
Asp tight
position
position
Central position(reference)

NOTE
If warning appears during bottom auto detection, please
click OK to clear it. But if detection failed, it might be that
the connection of the manifold is contaminated, leading to
poor contact. Please refer to 4.2.2 for details on
maintaining the connection of the manifold. Click EXIT to
return, or click OK to start the Bot Manu Ht.

11 To manually adjust the bottom aspiration height, press OK to highlight

Bot Manu Ht. The needles move to the inside of the first row of the
plate.

12 Channel XX
Cent Pos Left Pos
Right Pos Disp Pos
Bot Auto Ht Bot Manu Ht

Basic Operations 3-9

 12 Move the manifold upwards or downwards by selecting or
until the aspirate needles reach the appropriate aspiration height. The
position value is displayed on the upper right corner of the screen.
(Select to move upward and to move downward.)
Dispense needle Aspirate needle

Home
position

Aspiration
height
(reference)

Asp left
Asp tight
position
position
Central position(reference)

NOTE
You are recommended to use Bot Auto Ht, by which you
can get precise aspiration height. If you want to set the
aspirate height manually, you should adjust the aspirate
needles to be at most 0.4mm away from the microwell
bottom.

13 If you want to continue to set other parameters, press OK.

14 Otherwise, press EXIT to save your settings and return to the main
screen.

Parameter Position
Wash Maintenance

CAUTION
You must adjust all position parameters strictly as instructed above;
otherwise the instrument performance may be compromised.

3-10 Basic Operations

 NOTE
After any adjustment, you can press EXIT to return to the main
screen or press other keys to start corresponding operations.

3.3 Parameters

3.3.1 Parameter Configuration Screen

On the parameter configuration screen, you can adjust and set the following
parameters, which include program number, well bottom shape, wash solution,
wash mode, bottom wash, crosswise aspiration, dispensing volume, soak time,
shaking time, aspiration time and cycle times.

Press the PARA key on the front panel of the washer. The parameter
configuration screen is displayed as follows.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

A complete wash program includes:

„ Prog xx: Wash program number.

„ Flat: Well bottom shape. You can toggle between Flat and UV.
„ Wash X: Type of wash solution. You can toggle between Wash1 and Wash2.
„ Plate: Wash mode. You can toggle between Plate and Strip.
„ Bottom: You can activate or deactivate the bottom wash function.
„ Crosw Asp: You can activate or deactivate the crosswise aspiration function.
„ Vol XXX: Dispensing volume (μl). Adjustable from 50-3000μl in 50μl
increment.
„ Soak XXX: Soak time(s). Adjustable from 0-300s in 1s increment.
„ Shake XX: Set the shaking time(s), which is adjustable from 0-300s with
increment of 1s.
„ Asp XXX: Aspiration time(s). Adjustable from 0.1-10s in 0.1s increment.
„ Cyc XX: Cycle times of washing. Adjustable from 0-9 in 1 increment.

„ ：Manifold status check (selectable). You can select (no check) or

(check)

NOTE
Up to 100 wash programs (No.00-No.99) can be stored in the
washer.

Basic Operations 3-11

3.3.2 Setting up Programs
Each program is indicated by a unique number within 00-99 and convenient for
calling by user.

To set up a program:

1 Press the PARA key on the front panel of the washer. The program
setup screen is displayed. Prog xx is highlighted by default.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

2 Use and to define a new program number. Press to

increase or to decrease.

NOTE
If the selected program number is a new number, the
default parameters are displayed on the screen.

3 Press OK to highlight the well bottom shape.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

4 Select Flat or UV by pressing or .

5 Press OK to highlight the wash solution.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

6 Select Wash1 or Wash2 by pressing or .

NOTE
You should connect Wash1 and Wash2 to the
corresponding connectors on the rear panel of the washer.

7 Press OK to highlight the wash mode.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

3-12 Basic Operations

8 Select Plate or Strip by pressing or .

NOTE
You can only select Strip when using 48-well microplates.

9 Press OK to highlight the bottom wash.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

10 Select Bottom or No Bot by pressing or .

NOTE
The dispensing volume for bottom wash cannot be defined
and is about 150μl.

11 Press OK to highlight the aspiration mode.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

12 Select Crosw Asp or Std Asp by pressing or .

NOTE
You must select Std Asp for UV bottom plates.

13 Press OK to highlight the dispensing volume.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

Basic Operations 3-13

 14 Set the dispensing volume (μl) with increment of 50. Press to
increase or to decrease.

NOTE
The dispensing volume range is 50-3000μl with increment
of 50μl. The default dispensing volume is 300μl and the
capacity of each well is about 350μl.
When the dispensed volume is greater than 300μl, the
aspirate needles must not be too far away from the well;
otherwise overflow may be caused. You are
recommended to adjust the aspirate needles to be at most
±0.5mm away from the well.
Dispense needle Aspirate needle

Home
position

Left position

Dispensing
height
(reference)
Right position

Adjustment
range
(reference)

Microwell

Central position(reference)

15 Press OK to highlight the soak time.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

16 Increase or decrease the soak time(s) by pressing or . 0

means no soaking.

3-14 Basic Operations

17 Press OK to highlight the shaking time.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

18 Increase or decrease the shaking time(s) by pressing or . 0

means no shaking.
19 Press OK to highlight the aspiration time.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

20 Increase or decrease the aspiration time(s) by pressing or .

NOTE
The aspiration time is crucial to washing effects and 1.5s
by default. You can adjust the aspiration time as needed,
and it should be no less than 1s.

21 Press OK to highlight the cycle times.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

22 Increase or decrease the cycle times by pressing or .

23 Click OK to move the cursor on the manifold status check screen.

ProgXX Flat WashX

Plate Bottom Crosw Asp
VolXXX SoakXXX ShakeXX
AspXXX CycXX

Basic Operations 3-15

 24 Use or to select whether status check is enabled. ( :
check; : no check). In general, no check mode is selected.

NOTE：
If things like removing manifold during wash do not
happen, in the meantime standard micro plate is used with
no conspicuous dimension difference between the
manifold and the micro plate, the default mode—No check
can be used. If check mode is selected, when the
connection of the manifold is contaminated, the following
information will be displayed during washing:

Error!
Please check the manifold !
Press OK to continue or
EXIT to cancel !

It is necessary to check whether the manifold is in the right

position or the connection position of the manifold. Please
refer to 4.2.2 for details of the method.

25 If you want to set other programs or parameters, press OK.

26 Otherwise, press EXIT to save your settings and return to the main
screen.

Parameter Position
Wash Maintenance

NOTE
After any configuration, you can press EXIT to return to the main
screen or press other keys to start corresponding operations with
your settings saved automatically.

3.4 Running a Program

The program selection screen enables you to select a program and rows, and
to activate/deactivate the pre-rinse function.

CAUTION
Prior to running a program, check if there is enough wash solution
in the wash bottles and if the waste bottle is emptied.
You must run a pre-rinse before running the first wash program
when,
„ The instrument is started up; or
„ The wash solution is replaced.

3-16 Basic Operations

 NOTE
Different microplates may be different in height. Before using the
plates, be sure to set them level and make sure no microwell is
protruded.
The wash screen displays the parameters of wash program you
have set. Please check the parameters before starting the program
and change them if needed.

To run a program:

1 Press the WASH key on the front panel of the washer. The wash screen
is displayed as follows. The program number is highlighted by default.

XX Flat Wash X Plate

Bottom Crosw Asp Vol XXX
Soak XXX Shake XXX Asp XX
Cyc X Pre-rinse RowsX

2 Increase or decrease the program number by pressing or .

NOTE
The default program is the one that you’ve just complied or
used recently.

3 Press OK to highlight the pre-rinse item.

XX Flat Wash X Plate

Bottom Crosw Asp Vol XXX
Soak XXX Shake XXX Asp XX
Cyc X Pre-rinse RowsX

4 Select Pre-rinse or No Pre-R by pressing or .

WARNING
You must run a pre-rinse before running the first wash
program when,
„ The instrument is started up; or
„ The wash solution is replaced.

5 Press OK to highlight the rows number.

XX Flat Wash X Plate

Bottom Crosw Asp Vol XXX
Soak XXX Shake XXX Asp XX
Cyc X Pre-rinse RowsX

Basic Operations 3-17

 6 Increase or decrease the rows number by pressing or .

NOTE
The default rows number is 8 for 12-channel manifold. If
8-channel manifold is selected, the default rows number
will be 12. You can set the rows number as needed.

7 If you want to reselect a program or change the parameters, press OK.

8 Otherwise, press WASH to run the wash program.

NOTE
If the check mode is selected, prior to and during washing
the plate, the system will check the position of the manifold.
If a message “Please check the manifold! Press OK to
continue or EXIT to cancel!” pops up, it might be that the
manifold is not correctly placed or the connector of the
manifold is dirty. Follow instruction of 4.2.2 to clean it. Press
Ok to start the washing, Press EXIT to return to the main
screen. In general, No check is selected.
During the washing process, press EXIT to pause. To
resume the washing, press OK or press EXIT to return to
the main screen.

During washing process, the screen shows the current operation status, cycle
times and rows number. The operation status includes Starting air pump
Pre-rinsing, Aspirating, Dispensing, Soaking, Shaking, etc. Cyc X/X indicates
the current cycle and the total cycles. Rows X/X indicates the current row and
the total rows to be washed.

XXXXXXXXX
Cyc X/X Row X/X
Press EXIT to pause !

CAUTION
If the plate is not loaded properly, do not run wash programs.
Otherwise liquid may be spilled into the instrument and even lead to
electric shock or equipment damage.
Running a program when the wash bottles are lack of wash solution
may compromise the washer’s reliability and cause washing failure.
NOTE
The washer will display a prompt message and pause the operation
to remind you to take actions when the waste in the waste bottle
exceeds the limit line.

3-18 Basic Operations

 When the EXIT key is pressed during washing, the pause screen is displayed
as follows, indicating the washing status and asking you to taking an action.

Pausing
Cyc X/X Row X/X
Press OK to continue or
EXIT to cancel !

3.5 Maintenance
The fluidic tubing should be rinsed with distilled water or deionized water
periodically in order to prevent crystal deposits, thus eliminating equipment
failure.

NOTE
We recommend that you perform a daily rinse prior to turning off
the washer or a periodical rinse procedure.

To rinse the fluidic tubing:

1 Press the MAINT key on the front panel. The rinse setup screen is
displayed as follows.

Rinse time XX
Ensure Wash 2 is distilled
water! Press MAINT to Rinse
or EXIT to cancel !

2 Increase or decrease the rinse time(s) by pressing or .

NOTE
You should use Wash2 to rinse the fluidic tubing. Check if
the Wash2 bottle is filled with distilled water (or deionized
water). If not, you’ll not obtain satisfactory rinse effects. The
rinse time can be adjusted from 1s to 300s. The default is
10s. You are recommended to set the rinse time to no less
than 7s.

During rinsing process, the screen displays as follow:

In rinsing
Press EXIT to pause !

Press the EXIT key during rinsing. The following screen is displayed.

Basic Operations 3-19

 Pausing
Press OK to continue or
EXIT to cancel !

Press OK to continue rinsing, or press EXIT to abort

3.6 Waste Full

When the waste bottle is full, an alarm will be triggered and the screen displays
as follows:

Warning !
Waste full ! Press OK to
continue or EXIT to cancel !

You can remove the error using the following methods:

1 If the error occurs when wash solution is not dispensed, you should
pause or cancel the wash program, then empty the waste bottle and
restart the wash program.
2 If the error occurs during washing process and the sample can be
soaked for a long time, you can pause the operation and empty the
waste bottle and then continue with the wash program.
3 If the error occurs during washing process and the sample must not be
soaked for a long time, you can continue the washing in case: 1) the
waste in buffer bottle is less than 600ml; 2) dispensing volume is no more
than 350μl/well, 3) cycle times are less than 6. After this wash program,
you should empty the waste bottle and buffer bottle completely.
4 If the above-mentioned 3 conditions do not apply, you must first empty
the waste bottle and then continue the wash program.

BIOHAZARD
Dispose of the reagent, sample, wash solution and waste in
accordance with local or federal guide lines for biohazardous waste
disposal and consult the manufacturer or distributor of the reagents
for details. While disposing of the waste, wear gloves and lab coat
and, if necessary, goggles.
In case your skin contacts the waste, follow standard laboratory
safety procedures and consult a doctor.
CAUTION
Before running a wash program, be sure to check the waste bottle
for overflow and empty it if necessary; otherwise the instrument
performance may be compromised.
.

3-20 Basic Operations

 4 Maintenance
4.1 Daily Maintenance........................................................................................4-3
4.2 Weekly Maintenance ....................................................................................4-3
4.2.1 Maintaining the Solenoid Valve Tubing ..........................................4-3
4.2.2 Maintaining the manifold connector................................................4-4
4.3 Monthly Maintenance ...................................................................................4-5
4.4 As-Needed Maintenance ..............................................................................4-5
4.4.1 Cleaning Fluidic System.................................................................4-5
4.4.2 Cleaning Mechanical Parts.............................................................4-5

Maintenance 4-1
4-2 Maintenance
 4 Maintenance

To ensure the washer works normally, you must follow this procedure to
maintain it properly.

BIOHAZARD
When performing maintenance, do not touch any wash solution or
waste fluid with your hands. Wear gloves and lab coat and, if
necessary, goggles.
In case your skin contacts the waste fluid, follow standard
laboratory safety procedure and consult a doctor.

4.1 Daily Maintenance

„ Remove the residual fluid on the washer surfaces.
„ Before turning off the washer, launch a rinse procedure with distilled water or
deionized water to prevent crystal deposits.
„ Empty the waste bottle.

CAUTION
The wash solution contains phosphate and may result in crystal
deposits, thus clogging the tubing or connectors inside the washer.
Don’t leave wash solution in the tubing for a long period of time. A
rinse procedure is required everyday when the wash program is
finished. If the tubing and needles are plugged by crystal deposits,
clog them with the stainless needle provided with the washer. If the
crystal deposits cannot be removed due to no cleaning for a long
time, replace the manifold.

4.2 Weekly Maintenance

4.2.1 Maintaining the Solenoid Valve Tubing

The tubing clamped by the solenoid valves on the rear panel is easy to be
deformed. Pull and restore the tubing frequently to prevent deformation, or even
clogging and extend its service life.

Follow this procedure to maintain the tubing clamped by the solenoid valves:

Maintenance 4-3
 1 As for tubing clamped by Solenoid Valve 1, first pull the outer tubing a
little, then keep pressing the black end of Valve 1 and pull the inner
tubing.
2 As for tubing clamped by Solenoid Valve 2 first perform a rinse
maintenance for about 20s, then pull the tubing quickly when the
dispense needle is dispensing liquid.

NOTE
The tubing used together with Solenoid Valve 1, and Solenoid
Valve 2, has a service life of about 18 months if maintained as
required by Mindray. Check the tubing frequently for damage and
replace it if any.

4.2.2 Maintaining the manifold connector

Use cotton balls dipped with ethanol to wipe the manifold screws and metal
plates in the grooves of the manifold support, to prevent poor contact resulted
from dust accumulation.

NOTE
If used for a long time, the wash solution might adhere to the
manifold retaining screw or metal plate due to spillage and
evaporation. The connector of the manifold should be cleaned in
time to avoid rust or crystallizing affection washing effect.

4-4 Maintenance
4.3 Monthly Maintenance
When used for a long period, the wash bottle, waste bottle, buffer bottle and
distilled water bottle may have crystal deposits. Follow this procedure to wash
the bottles every month:
1 Fill each bottle with 0.8L deionized water (40℃) and tighten the bottle
cap, then shake the bottle.
2 Remove the bottle cap and use a brush to clean the inside wall and
cap of the bottle. While cleaning, ensure no waste fluid is spilled out.

4.4 As-Needed Maintenance

BIOHAZARD
Wear gloves while performing the cleaning.
Prior to any servicing or shipment, the instrument should be
disinfected and cleaned to prevent from biohazardous infection.

4.4.1 Cleaning Fluidic System

1 Fill wash2 bottle with sufficient distilled water or deionized water at
about 40℃.
2 Run a rinse procedure for about 30s to clean the tubing and manifold
thoroughly. The rinse procedure should be launched for at least once.
3 Use hot water (40℃) to wipe the external surfaces of the bottles and
tubing.

4.4.2 Cleaning Mechanical Parts

1 Turn off the system and unplug the power cord.
2 Remove the manifold and wipe the metal plates in the grooves using
clean cotton swab dipped with ethanol (50%), then wipe the surfaces of
the manifold support.
3 Wipe the retaining screws on manifold using clean cotton swab dipped
with ethanol (50%), then wipe the surfaces of the dispense needles,
aspirate needles and entire manifold.
4 Use clean cotton swab dipped with ethanol (50%) to wipe the surfaces
of the plate carrier.

Maintenance 4-5
 WARNING
The needles and leaf spring are edged and can cause scratches to
human body. Exercise caution while performing the cleaning job.

4-6 Maintenance
 5 Troubleshooting
5.1 Manifold Failure............................................................................................5-3
5.2 Movement Error............................................................................................5-4
5.3 Dispense Needle Error .................................................................................5-4
5.4 Residue Too Much........................................................................................5-5
5.5 Wash Solution Overflow ...............................................................................5-6
5.6 Collision of Manifold and Microplate.............................................................5-6

Troubleshooting 5-1
5-2 Troubleshooting
 5 Troubleshooting
NOTE
When alarm occurs, press any key to cancel the alarm and operate
according to the screen prompts.

5.1 Manifold Failure

If the check mode is selected, when the connection of the manifold is
contaminated or the manifold is not in the right position, the following
information will be displayed during washing:

Error!
Please check the manifold !
Press OK to continue or
EXIT to cancel !

You can follow this procedure to recover the failure:

1 Check if the manifold is placed correctly and not tilted, place it level if
needed. Press OK and check if the instrument works normally; or
2 Lift up the manifold, then wipe the retaining screws and metal plates with
clean cotton cloth dipped with ethanol. Install the manifold properly and
press OK; or

3 Restart the washer and run the wash program correctly.

4 If things like removing manifold during wash do not happen, in the
meantime standard micro plate is used with no conspicuous dimension
difference between the manifold and the micro plate, the default

Troubleshooting 5-3
 mode—No check（ ）can be used.
5 If the error remains, please contact Mindray Customer Service
Department or your local distributor.

5.2 Movement Error

When error occurs during startup or other movement, the screen displays as
follows:

Error!
XXXX movement error !
Restart or see the user
manual !

“XXXX” refers to horizontal or vertical.

You can correct the error using the following methods:

1 Switch off the power and restart the instrument; or

2 Switch off the instrument power. In case of horizontal movement error,
move the plate carrier back and forth for 5 times. In case of vertical
movement error, move the manifold up and down for 5 times. Then
restart the instrument and check if it works normally.
3 If the error remains, please contact Mindray Customer Service
Department or your local distributor.

5.3 Dispense Needle Error

If some dispense needle dispenses little or no wash solution during washing
process, you can restore the error using the following methods:

1 Check if the wash bottle is connected to the washer correctly. Reconnect

the tubing if needed; or
2 Check if the fluidic tubing is creased, stretch it if necessary; or
3 The fluidic tubing may adhere to each other if not moved for a long time.
As for tubing clamped by Solenoid Valve 1, first pull the outer tubing a
little, then keep pressing the black end of Solenoid Valve 1 and pull the
inner tubing. As for tubing clamped by Solenoid Valve 2, first run a rinse
procedure for about 20s, then pull the tubing quickly when the dispense
needle is dispensing liquid; or

5-4 Troubleshooting
 4 Check if the tubing end inside the wash bottle is blocked. If it is, remove
the bottle cap and then unclog the tubing; or
5 Check if the manifold inlet and dispense needles are clogged. If they are,
remove the foreign matters from the manifold inlet using tweezers, and
use the supplied stainless needle to unclog the blocked dispense
needles; or
6 Interchange the dispensing tubing and the waste tubing, then press
MAINT to backflush the dispensing tubing with air flow, drawing the
foreign matters into the waste bottle. Reconnect the tubing correctly and
run a rinse procedure again.
7 If the error remains, please contact Mindray Customer Service
Department or your local distributor.

5.4 Residue Too Much

When excessive residue exists in microwell during washing process, you can
correct the error using the following methods:

1 Check if the waste/buffer bottle caps are tightened or sealed, or the

fluidic tubing is connected properly to the bottle cap. Tighten the bottle
caps and reconnect the fluidic tubing to the bottles; or
2 Check if the distance between aspirate needle and microwell bottom is
too large and not within 0.1-0.4mm. You’re recommended to set the
bottom wash height using the Bot Auto Ht function.
3 Check if the fluidic tubing is creased, stretch it if necessary; or
4 Check if the tubing end inside the waste or buffer bottle is blocked. If it is,
remove the bottle cap and then unclog the tubing; or
5 Check if the manifold inlet and aspirate needles are clogged. If they are,
remove the foreign matters from the manifold inlet using tweezers, and
use the supplied stainless needle to unclog the blocked aspirate needles;
or
6 If the manifold has not been used for a long time, the connector might get
rusty, leading to the change or lifting of the connector. Follow the 4.2.2 to
clean the connector of the manifold. If the rust is very serious, use
abrasive paper of small granule to rub the rust away from the manifold
retaining screw.
7 Interchange the aspiration tubing and the waste tubing, then press
MAINT to backflush the waste tubing with distilled water. Reconnect the

Troubleshooting 5-5
 tubing correctly and run a rinse procedure again.
8 If the error remains, please contact Mindray Customer Service
Department or your local distributor.

5.5 Wash Solution Overflow

If the dispense needles dispense excessive wash solution into the microplate
wells, resulting in overflow, you can correct the error using the following
methods:

1 Check if the waste/buffer bottle caps are tightened or sealed, or the

fluidic tubing is connected properly to the bottle cap. Tighten the bottle
caps and reconnect the fluidic tubing to the bottles; or
2 Check if the fluidic tubing is creased, stretch it if necessary; or
3 Check if the aspirate needles are far from the well. Check the dispensing
height and adjust the Disp Pos correctly; or
4 Check if foreign matters exist at Solenoid Valve 2 on the rear panel of the
washer, disabling the valve core to clamp the tubing tightly. Remove the
tubing clamped by Solenoid Valve 2 according to 4.2 Weekly
Maintenance and remove the foreign matters.
5 If the error remains, please contact Mindray Customer Service
Department or your local distributor.

5.6 Collision of Manifold and Microplate

If the manifold collides with the microplate, causing the manifold to fall off or the
plate is tilted, you can handle the error using the following methods:

1 If the microplate is not placed properly on the carrier, reset the plate and
attach it to the carrier surface completely; or
2 Check the manifold position is set properly. Reset the crosswise
aspiration positions (Left Pos and Right Pos), ensuring the aspirate
needles lie inside the wells. Also check if the dispensing height is set
correctly; or
3 Check if the plate carrier or manifold support is blocked. Remove the
foreign matters if any.
4 If the error remains, please contact Mindray Customer Service
Department or your local distributor.

WARNING
Servicing the instrument can be performed only by technical
personnel approved by Mindray.
You must troubleshoot the instrument strictly as instructed by the
operator’s manual.

5-6 Troubleshooting
CAUTION
Use the consumables recommended by Mindray. Other
consumables may degrade the system performance.
NOTE
The service life of consumables like plastic bottles, connectors or
manifold is about 24 months and that of tubing is about 18 months.
Please check the consumables and tubing frequently and replace
them if necessary.

Troubleshooting 5-7
5-8 Troubleshooting
 6 Appendix
Appendix A Specifications .................................................................................6-3
Appendix B Supplies .........................................................................................6-4
Appendix C Index ..............................................................................................6-5

Appendix 6-1
6-2 Appendix
 Appendix A Specifications

Environment Indoor
Power supply 100-240V~, 50/60Hz
Power consumption: 100VA
Dimensions 400 mm×330 mm×145 mm (L×D×H)
Weight 7.2kg
Display 192×64 dot matrix LCD
Keypad consists of 8 functional keys
Operating 5°C-35°C
temperature
Storage 0°C-40°C
temperature
Relative 15%-85%
humidity
Operating -400m-3,000 m (-1,300 feet-6,500 feet)
altitude
Atmospheric 70kPa-106kPa
pressure
Communication RS-232
interface
EMC The equipment complies with the emission and immunity
requirement description in this part of the EN 61326-1:2006
and EN 61326-2-6: 2006.

Appendix 6-3
 Appendix B Supplies

To ensure personal safety and system performance, use only supplies

manufactured or recommended by Mindray. Contact the Mindray Customer
Service Department or your local distributor for details.

6-4 Appendix
 Appendix C Index

dispense needle .. 3-4, 3-8, 4-4, 4-5,

Ａ 5-4, 5-5, 5-6
aspirate 1-3, 3-4, 3-5, 3-6, 3-7, 3-8, dispensing position ........... 3-4, 3-8
3-10, 3-14, 4-5, 5-5, 5-6
dispensing volume3-11, 3-13, 3-14,
aspirate needle ... 3-4, 3-5, 3-6, 3-7, 3-20
3-8, 3-10, 3-14, 4-5, 5-5, 5-6

aspirate position........................ 3-4 Ｈ

aspiration .... 3-3, 3-4, 3-6, 3-7, 3-9, handle.......................... 4, 3-20, 4-3
3-10, 3-11, 3-13, 3-15, 5-5

aspiration height .......3-4, 3-9, 3-10 Ｌ

Left Pos...................... 3-4, 3-6, 5-6

Ｂ

Bot Auto Ht ...... 3-4, 3-8, 3-10, 5-5 Ｍ

Bot Manu Ht...................... 3-4, 3-9 maintenance . 1, 1-4, 3-19, 4-1, 4-3,
4-4, 4-5, 5-6
bottle... 3, 5, 1-3, 1-4, 2-5, 2-6, 3-5,
3-16, 3-18, 3-19, 3-20, 4-3, 4-5, manifold..... 4, 5, 1-3, 1-4, 2-4, 2-5,
5-4, 5-5, 5-6 2-6, 3-4, 3-6, 3-8, 3-10, 3-18,
4-3, 4-4, 4-5, 5-3, 5-4, 5-5, 5-6,
bottom shape ................. 3-11, 3-12 5-7

bottom wash ...........3-11, 3-13, 5-5 manifold support .2-4, 4-4, 4-5, 5-6

Ｃ Ｎ

Cent Pos .....................3-4, 3-5, 3-6 needle position ..........................3-4

central position ......................... 3-4 Ｐ

Channel .................................... 3-4
pre-rinse ........................ 3-16, 3-17
crosswise aspiration..3-4, 3-11, 5-6
program1-3, 3-11, 3-12, 3-16, 3-17,
Cycle ...................................... 3-11 3-18, 3-20

Ｄ Ｒ

Disp Pos .....................3-4, 3-8, 5-6 retaining screw........... 2-4, 4-5, 5-3

dispense ...... 1-3, 2-5, 3-3, 3-4, 3-8, Right Pos.................... 3-4, 3-7, 5-6
3-11, 3-13, 3-14, 3-18, 3-20, 4-4,
rinse 1-4, 1-5, 2-5, 3-3, 3-17, 3-19,
4-5, 5-4, 5-5, 5-6
4-3, 4-4, 4-5, 5-4, 5-5, 5-6

Appendix 6-5
 rows ..................... 3-16, 3-17, 3-18 Ｗ

wash mode..................... 3-11, 3-12

Ｓ
wash program ...1-4, 1-5, 3-3, 3-11,
shaking...........................3-11, 3-15
3-16, 3-17, 3-18, 3-20, 4-3, 5-3
Soak ........................................3-11
wash solution.......5, 1-3, 1-4, 3-11,
solenoid valve ...........................4-3 3-12, 3-16, 3-17, 3-18, 3-20, 4-3,
5-4, 5-6
stainless needle ................. 4-3, 5-5
waste...2, 3, 4, 5, 1-3, 1-4, 2-4, 2-5,
2-6, 3-5, 3-16, 3-18, 3-20, 4-3,
4-5, 5-5, 5-6

6-6 Appendix
P/N: 0105-20-63148 (4.0)