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Spectral Analysis - PDF: Open With

The document discusses several analytical techniques used for spectral analysis including: 1) Ultraviolet-visible spectroscopy, infrared spectroscopy, and nuclear magnetic resonance spectroscopy and their principles and applications. 2) Chromatography techniques including column, flash, vacuum liquid, HPLC, TLC, and GC-MS and their separation mechanisms. 3) Thermal techniques such as DSC, DTA, TGA, and ORD. 4) Electrophoresis techniques including capillary and gel electrophoresis.

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Anjali Sharma
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49 views

Spectral Analysis - PDF: Open With

The document discusses several analytical techniques used for spectral analysis including: 1) Ultraviolet-visible spectroscopy, infrared spectroscopy, and nuclear magnetic resonance spectroscopy and their principles and applications. 2) Chromatography techniques including column, flash, vacuum liquid, HPLC, TLC, and GC-MS and their separation mechanisms. 3) Thermal techniques such as DSC, DTA, TGA, and ORD. 4) Electrophoresis techniques including capillary and gel electrophoresis.

Uploaded by

Anjali Sharma
Copyright
© © All Rights Reserved
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Download as PDF, TXT or read online on Scribd
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3/14/2018 SPECTRAL ANALYSIS.

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SPECTRAL ANALYSIS

1. ultra violet & visible spectroscopy :


basic principle ,characteristics region of spectrum, energy levels & selection rules,
woodward – fieser , influence of substituent ,ring size & strain
on spectral characteristics , solvent effect non-conjugated interaction ,spectral correlation
with structure , uv- values .
(ref.pavia )
2. infrared spectroscopy :
characteristics region of spectrum , influence of substituents , ring size , hydrogen bond ,
vibrational coupling & field effect on frequency . spectral interpretation , ir value.
.(ref.pavia )
3. Nulear magnetic resonance spectrum :
Magnetice nuclei , chemical shift & shielding , relaxation ,processes ., chemical &^
magenetce non equivanence , local dinagnetce shielding , magnetice anisotropy , spin –
spin splitting , pascal triangle , coupling constant .
.(ref.pavia )
Application of raman spectra nicely.

c)Analytical techniques
1. chromatography : ( only go for basics)
general principles , classification , normal & reversed phase , bonded phase,
sepration mechanisms .
types: a. column chroma tography .
b. flash chromatography .
c. vaccum liquid chromatography .
d. hplc
e. tlc, hptlc, oplc (over pressure layer chromatography )
f. centrifugal chromatography .
g. counter – current chromatography .
h. droplet – counter current chromatography .
i. ion exchange , affinity , size exclusion & ion pair chromatography
perfusion , fast protein liquid chromatography, supercritical chrmo.
j. gc, gc-ms, lc- ms .
2. Thermal techniques :dsc, dta, tga .ord , cd (Very nicely) .
3. Electrophoresis : capillary, gel .
4. other : crystallography , spectrofluorimetry

Metries like potentiometry, polarimetry only imp equations and reference standard and imp
applications if any...

Refer standard books like pavia, kemp, skoog...

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