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Worksheet No. 1 Paper Chromatography: Observations

The document summarizes a paper chromatography experiment performed by students to separate pigments in ink samples. During the experiment, the chromatogram paper developed spots as the solvent traveled up the paper, separating the different pigments. Based on the colors observed, Sample 1 contained a pure pink pigment, Sample 2 was a mixture with blue and violet pigments, and Sample 3 contained a pure green pigment. The procedure can identify unknown pigments by how far each separates from the start line using a mobile solvent phase. A pencil was used to draw lines instead of ink to avoid dissolving in the organic solvent.

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Neil Penetrante
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1K views1 page

Worksheet No. 1 Paper Chromatography: Observations

The document summarizes a paper chromatography experiment performed by students to separate pigments in ink samples. During the experiment, the chromatogram paper developed spots as the solvent traveled up the paper, separating the different pigments. Based on the colors observed, Sample 1 contained a pure pink pigment, Sample 2 was a mixture with blue and violet pigments, and Sample 3 contained a pure green pigment. The procedure can identify unknown pigments by how far each separates from the start line using a mobile solvent phase. A pencil was used to draw lines instead of ink to avoid dissolving in the organic solvent.

Uploaded by

Neil Penetrante
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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WORKSHEET NO.

PAPER CHROMATOGRAPHY

Name: Penafiel, Penetrante, Porol, Privado, Rivera, Rosales


_____________________________________ Score: _________
Section: SAR22
_______________ Date Performed: ______________
2
Group No: _______________ Teacher: ____________________

OBSERVATIONS
1. The chromatogram paper

1.1 How did the appearance of the chromatogram


paper change during the progress of the
chromatographic procedure?
As the solvent slowly travels up the paper,
the chromatogram paper was spotted with
different pigments that the ink contains

1.2 Describe briefly how this chromatogram would


have been produced.
The mixture of pigments from the pen were
separated by using two types of substance:
mobile phase (solvent) and stationary phase
(chromatogram paper)

2. Based from the chromatogram classify the samples.


Color of pigment/s Classification of sample (pure
Sample
observed substance/ mixture)
1 Pink Pure Substance
2 Blue/Violet Mixture
3 Green Pure Substance

Post-Laboratory Questions:

1. How will you use this procedure to identify an unknown type of colored pigment?
- An unknown type of colored pigment can be identified by using a (mobile phase) solvent that is
capable of transporting the solution being tested.
2. Why pencil is used in drawing the start line and stop line and not an ink pen?
- Graphite from the pencil lead doesnt dissolve in organic solvents unlike the inks from pens.

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