11/30/2016
MONDAY, NOVEMBER 14TH, 2016
Review Question:
Agenda:
Case Study
CHAPTER 6
A Tour of the Cell
With your table partner, match the parts!
TUESDAY, NOVEMBER 15TH, 2016
Review Question:
Agenda:
Finish case study
Reading Guide for Chapter 6
Mastering Biology Quiz is due Friday at 11:59
Lab: How to use a microscope
Body Tube
Nosepiece
Objectives
Ocular lens
(Eyepiece)
Arm
Stage
Stage Clips
Diaphragm
Light
Whats my power?
To calculate the power of magnification, multiply the power of the
ocular lens by the power of the objective.
Coarse Adjustment
What are the powers of
magnification for each of
the objectives we have on
our microscopes?
Fine Adjustment
Base
Always carry a microscope with one hand
holding the arm and one hand under the base.
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How to Focus a Microscope:
Comparing Powers of Magnification
Step 1. Stage at lowest position and objective on the red (4x). This is storing position!
Step 2: Use coarse objective to focus image.
We can see better details with higher the
powers of magnification, but we cannot see
as much of the image.
Step 3: Turn to yellow (10x) objective. DO NOT TOUCH THE COARSE ADJUSTMENT.
Step 4: Use FINE ADJUSTMENT to focus image.
Step 5: Carefully turn to blue (40x) objective. DO NOT TOUCH THE COARSE ADJUSTMENT.
Step 6: Use FINE ADJUSTMENT to focus image. 40x objective may not fit, dont force it.
Which of these images
would be viewed at a
higher power of
magnification?
TIPS:
1. Go through all of the steps and dont see anything? Start over from the beginning!
2. DO NOT use the coarse adjustment on any objective except the RED 4x objective.
Safety concerns when working with slides
Slides are GLASS: they are fragile and easy to
break. Handle with care!
WEDNESDAY, NOVEMBER 15TH, 2016
Limits to cell size lab
Please dont use the coarse (big) adjustment on
the highest (blue) objective!
If you break a slide, let Mrs. Pagett know right
away so it can be cleaned up. Do not attempt to
clean up the glass by yourself!
THURSDAY, NOVEMBER 17TH, 2016
Review Question: Which structure is common to
plant and animal cells?
A. Chloroplast
B. Cell wall made of cellulose
C. Central Vacuole
D. Mitochondrion
E. Centriole
Agenda:
A few notes
Baggie Cells
Cell Race
YOU MUST KNOW
Three differences between
prokaryotic and eukaryotic cells.
The structure and function of
organelles common to plant and
animal cells.
The structure and function of
organelles found only in plant cells
or only in animal cells.
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A PROKARYOTIC CELL (BACTERIA)
2 TYPES OF CELLS:
1. Prokaryotes: Domain Bacteria &
Archaea
2. Eukaryotes (Domain Eukarya):
Protists, Fungi, Plants, Animals
PROKARYOTE VS. EUKARYOTE
before
true
No
kernel
nucleus
DNA in a nucleoid
Cytosol
No organelles other
than ribosomes
Small size
Primitive
i.e. Bacteria & Archaea
Has
Cells
kernel
nucleus and nuclear
envelope
Cytosol
Membrane-bound
organelles with
specialized
structure/function
Much larger in size
More complex
i.e. plant/animal cell
must be small to maintain a large surface
area to volume ratio
Large S.A. allows rates of chemical exchange
between cell and environment
CELL SIZE AND SCALE
http://learn.genetics.utah.edu/content/begin/cells/scale/
Scale of the Universe:
http://www.onemorelevel.com/game/scale_of_the_unive
rse_2012
NUCLEUS
Function: control center of cell
Contains DNA
Surrounded by double membrane (nuclear envelope)
Continuous with the rough ER
Nuclear pores: control what enters/leaves nucleus
Chromatin: complex of DNA + proteins; makes up
chromosomes
Nucleolus: region where ribosomal subunits are
formed
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NUCLEUS
Contains DNA
Function: control center of cell
Surrounded by double membrane (nuclear envelope)
Continuous with the rough ER
Nuclear pores: control what enters/leaves nucleus
Chromatin: complex of DNA + proteins; makes up
chromosomes
Nucleolus: region where ribosomal subunits are
formed
RIBOSOMES
Function: protein synthesis
Composed of rRNA + protein
Large subunit + small subunit
Types:
1. Free ribosomes: float in cytosol, produce
proteins used within cell
2. Bound ribosomes: attached to ER, make
proteins for export from cell
ENDOPLASMIC RETICULUM (ER)
ENDOMEMBRANE SYSTEM:
Regulates protein traffic & performs
metabolic functions
ENDOPLASMIC RETICULUM (ER)
Network of membranes and sacs
Types:
1. Rough ER: ribosomes on surface
Function: package proteins for secretion,
send transport vesicles to Golgi, make
replacement membrane
2. Smooth ER: no ribosomes on surface
Function: synthesize lipids, metabolize
carbs, detox drugs & poisons, store Ca2+
GOLGI APPARATUS
Function: synthesis & packaging of materials (small
molecules) for transport (in vesicles); produce lysosomes
Series of flattened membrane sacs (cisternae)
Cis face: receives vesicles
Trans face: ships vesicles
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LYSOSOMES
Function: intracellular digestion; recycle cells
materials; programmed cell death (apoptosis)
Contains hydrolytic enzymes
VACUOLES
Function: storage of materials (food, water, minerals,
pigments, poisons)
Membrane-bound vesicles
Eg. food vacuoles, contractile vacuoles
Plants: large central vacuole -- stores water, ions
Parts of plant & animal cell p 108-109
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MITOCHONDRIA
Function: site of cellular respiration
Double membrane: outer and inner membrane
Cristae: folds of inner membrane; contains enzymes
for ATP production; increased surface area to ATP
made
Matrix: fluid-filled inner compartment
CHLOROPLASTS
ENDOSYMBIONT THEORY
Function: site of photosynthesis
Double membrane
Thylakoid disks in stacks (grana); stroma (fluid)
Contains chlorophylls (pigments) for capturing
sunlight energy
PEROXISOMES
Functions: break down fatty acids; detox alcohol
Involves production of hydrogen peroxide (H2O2)
Mitochondria & chloroplasts
share similar origin
Prokaryotic cells engulfed by
ancestors of eukaryotic cells
Evidence:
Double-membrane
structure
Have own ribosomes &
DNA
Reproduce independently
within cell
CYTOSKELETON: NETWORK OF PROTEIN FIBERS
Function: support, motility, regulate biochemical
activities
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FRIDAY, NOVEMBER 18TH, 2016
Review Question: Identify the structure labeled A and
explain how it is adapted for the organelle to function
efficiently.
SURFACE AREA EXAMPLE (ANIMAL):
Cristae (inner membrane) provide additional surface area
for the enzymatic reactions of respiration.
Agenda:
Discuss Baggie Cells
Discuss Cell Race
Start Chapter 7
Animal Care
FOLDS VILLI MICROVILLI
Small Intestine: highly folded surface to
increase absorption of nutrients
Villi: finger-like projections on SI wall
Microvilli: projections on each cell
SURFACE AREA EXAMPLE (PLANT):
Root hairs: extensions of root epidermal cells;
increase surface area for absorbing water and
minerals
INTERCELLULAR JUNCTIONS (ANIMAL
CELLS)
EXTRACELLULAR MATRIX (ECM)
Outside plasma membrane
Composed of glycoproteins (ex. collagen)
Function: Strengthens tissues and transmits external
signals to cell
Tight junctions: 2 cells
are fused to form
watertight seal
Desmosomes: rivets
that fasten cells into
strong sheets
Gap junctions:
channels through which
ions, sugar, small
molecules can pass
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PLANT CELLS
Plant Cells Only
Animals Cells Only
Cell wall: protect plant,
maintain shape
Composed of cellulose
Plasmodesmata:
channels between cells to
allow passage of
molecules
Central vacuoles
Lysosomes
Chloroplasts
Centrioles
Cell wall of cellulose
Flagella, cilia
Plasmodesmata
Desmosomes, tight and
gap junctions
Extracellular matrix
(ECM)
HARVARD CELL VIDEO
http://multimedia.mcb.harvard.edu/anim_innerlife
.html