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Department of Speech Language Pathology, College of Rehabilitation Sciences University of Santo Tomas, Espana Street, Manila 1008

1) Different amino acids were subjected to four chemical tests - Hopkins-Cole, Lead Acetate, Sakaguchi, and Xanthoproteic - to observe their reactions. 2) The tests identified characteristics like color changes for each amino acid. For example, tyrosine produced a white precipitate in the Lead Acetate test and a green color in the Sakaguchi test. 3) The results provided information on distinguishing the amino acids based on their properties and reactions to specific reagents. This helps characterize the molecular structures of the different protein building blocks.

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Raphaelle May Tan
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Department of Speech Language Pathology, College of Rehabilitation Sciences University of Santo Tomas, Espana Street, Manila 1008

1) Different amino acids were subjected to four chemical tests - Hopkins-Cole, Lead Acetate, Sakaguchi, and Xanthoproteic - to observe their reactions. 2) The tests identified characteristics like color changes for each amino acid. For example, tyrosine produced a white precipitate in the Lead Acetate test and a green color in the Sakaguchi test. 3) The results provided information on distinguishing the amino acids based on their properties and reactions to specific reagents. This helps characterize the molecular structures of the different protein building blocks.

Uploaded by

Raphaelle May Tan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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BIOORGANIC

CHEMISTRY
BioOrganic Chemistry Laboratory CH205 (2014 2015) Experiment 6

Jonas Clarence DJ. Tan, Raphaelle May C. Tan*, Edward Joseph A. Tanedo, Carmela L. Tria
Department of Speech Language Pathology, College of Rehabilitation Sciences
University of Santo Tomas, Espana Street, Manila 1008
Date Submitted: March 12,2015
Abstract:
Amino acids are the building blocks of protein and peptides. Different amino acids were put through
different chemical tests to describe each. During the experiment it was observed that some have reactions with some
reagents.
Keywords: Amino Acid, Protein ,Hopkins- Cole Test, Lead-Acetate Test, Sakaguchi test, Xanthoproteic Test

Introduction:
Amino acids are the building blocks proteins. Protein is one of the most important
biochemical molecules. The amino acids used in the experiment were Phenylalanine, Tyrosine,
Serine, Tryptopan, Arginine, Cysteine, Methionine and Histidine.
Phenylalanine is an essential amino acid. It is mostly gotten from food. Phenylalanine is
turned into Tyrosine by the body. Tyrosine is needed to help build proteins, neurotransmitters
melanin and hormones. The largest amino acid is Tryptohpan.
Methodology:
During the experiment, 4 types of specific tests for amino acids were performed namely
Hopkins- Cole Test, Lead Acetate Test, Sakaguchi Test and Xanthoproteic Test. These test were
performed to Phenylalanine, Tyrosine, Serine, Tryptophan, Arginine, Cysteine, Methionine and
Histidine.
The 10 drops of the test compounds were each placed in separate test tubes for the 4
different tests to be performed. A total of 32 test tubes were used to contain the amino acids.
For the Hopkins- Cole Test, 20 drops of Hopkins-Cole reagent was added to each of the
test compounds. Each test tube was to be mixed and be inclined at 45. 20 drops of concentrated
H2SO4 was carefully added to each test tube. The color during the interphase was noted.
In the Lead Acetate Test, 10 drops of Concentrates NaOH was added to the test tubes
with test compunds. The test tubes wer heated in a boiling water bath for 5 minutes. After which
5 drops of 1% Pb(Ac)2 was added. The color of the precipitate was noted.

During the Sakaguchi test, 10 drops of 10% NaOH and 10 drops of 0.02% ethanolic naphthol was added to the test tube with test compounds. The contents of the test tube was mixed
and after 3 minutes, 5 drops of 2% NaOBr solution was added. The color was noted.
The Xanthoproteic test had 10 drops of concentrated HNO3 to the test tubes. The test
tubes were heated in a boiling water bath until one test compound becomes colored. The color
was noted. Concentrated NaOH was added to the test compound until it became basic with the
litmus paper. The color was noted.
Results and Discussion:
Test Compounds
Phenylalanine

Hopkins- Cole
test
Colorless

Lead Acetate test

Sakaguchi test

Xanthoproteic
test
Pale yellow

White Cloudy
Colorless
Percipitate
Tyrosine
White
White Cloudy
Green
Reddish brown
Percipitate
Serine
Colorless
White Cloudy
Colorless
Colorless
Percipitate
Tryptophan
Yellow
White Cloudy
Goldish brown
Reddish brown
Percipitate
Arginine
Colorless
White Cloudy
Light orange
Colorless
Percipitate
Cysteine
Colorless
Gray Percipitate
Yellow
Colorless
Methionine
Colorless
White Cloudy
Colorless
Colorless
Percipitate
Histidine
Colorless
White Cloudy
Orange
Yellow
Percipitate
Table 1 Reactions of the different test compounds to the tests performed

Conclusion:
References:

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