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Distillase®

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1K views

Distillase®

Uploaded by

Rafael
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Product Information

DESCRIPTION
DISTILLASE

SSF is a system of enzymes designed


to produce glucose from liqueed corn or milo mash
during simultaneous saccharication and fermentation
(SSF) to ethanol. It contains: amylase (1,4--D-glucan
glucanohydrolase - EC 3.2.1.1), glucoamylase (1,4--D-
glucan hydrolyase E.C. 3.2.1.3) and Aspergillopepsin 1
(EC 3.4.23.18).
The enzymes contained in DISTILLASE

SSF are
produced by genetically modied strains of Trichoderma
reesei.
TYPICAL CHARACTERISTICS
Activity: 380 GAU/g (minimum)
Appearance: Brown liquid
pH: 4.0 - 4.5
Specic gravity: 1.10 - 1.14 g/ml
UNIT DEFINITION
One GIucoamylase Unit (GAU) is the amount of enzyme
that will liberate one gram of reducing sugars calculated
as glucose per hour from soluble starch substrate under
the conditions of the assay. A detailed assay method is
available upon request.
PERFORMANCE BENEFITS
The DISTILLASE

SSF systems unique composition


improves fermentation yield and consistency. It does
so by minimizing the fraction of starch that remains
unfermented and enhancing yeast nutrition.
The glucoamylase in DISTILLASE

SSF system rapidly


converts the dextrins to glucose across a broad range of
starting pH (4.0 to 5.8). This results in 5 - 10% faster initial
ethanol production.
The acid stable saccharifying amylase in the DISTILLASE


SSF effectively hydrolyzes starch that would not
otherwise be converted to glucose during fermentation.
The protease in the DISTILLASE

SSF system hydrolyzes


protein matrices in the corn kernel that bind the various
fractions enabling the hydrolysis of hard to hydrolyze
starch. It also increases the levels of essential yeast
nutrients in the form of amino acids, peptides and free
amino nitrogen.
APPLICATION RECOMMENDATIONS
DISTILLASE

SSF is used to saccharify liqueed mashes


of whole ground corn and milo. The resultant glucose is
fermented by yeast to produce ethanol. All of the
enzymes in DISTILLASE

SSF perform well within the


temperature and pH ranges typical in whole ground corn
and milo batch fermentations: 28 to 35C (82 to 95F)
and 4.0 to 5.5 respectively. In high gravity (>34% solids)
and/or milo fermentations performance can be further
enhanced by the addition of FERMGEN

acid
fungal protease.
DOSAGE RECOMMENDATIONS
DISTILLASE

SSF should be dosed at 0.06 to 0.08%


weight enzyme / weight as is corn. The actual dose
required will depend upon the conditions of your
fermentation: time, initial pH and the level of solids.
REGULATORY STATUS
DISTILLASE

SSF complies with current FAO/WHO


and FCC recommended specications for food-grade
enzymes and is GRAS (Generally Recognized As Safe) in
the United States for use in alcohol production.
The enzyme ingredients in DISTILLASE

SSF are in
compliance with the Toxic Substances Control Act in the
United States and Domestic Substances List in Canada.
PACKAGING
DISTILLASE

SSF is available in various package sizes


and bulk tankers. Please consult your Genencor
representative for detailed information.
STORAGE
DISTILLASE SSF will meet the declared activity upon
arrival at your plant. DISTILLASE

SSF can be safely


stored in sealed containers. Enzymes should be stored
below 25C (77F) and sheltered from direct sunlight for
maximum stability.
SAFETY & ENZYME HANDLING
Inhalation of enzyme dust and mists should be avoided.
In case of contact with the skin or eyes, promptly rinse
with water for at least 15 minutes.
For detailed handling information, please refer to the
appropriate Material Safety Data Sheet, the Enzyme
Technical Association (ETA) handbook Working Safely
With Enzymes, and the Association of Manufacturers
and Formulators of Enzyme Products (Amfep) handbook
Guide to the Safe Handling of Microbial Enzyme
Preparations. All are available from Genencor.
TECHNICAL SERVICE
Genencor is committed to working with ethanol
producers to achieve their goals. More detailed
information about the application of this product is
available upon request. If you have any questions, please
call us and let us know how we can be of assistance
Together we can.
DISTILLASE

SSF
Saccharifying System For Dry Grind Ethanol Production
Danisco US Inc. 2010. GENENCOR, DISTILLASE and
FERMGEN are registered trademarks of Danisco US Inc.
or its afliated companies.
USE AND/OR SALE OF THIS PRODUCT MAY BE COVERED BY ONE OR MORE PATENTS
IN THE U.S. AND OTHER COUNTRIES, AS WELL AS PENDING PATENT APPLICATIONS IN
THE U.S. AND OTHER COUNTRIES.
The information contained in this product literature is, to the best of our knowledge, true
and accurate and the product as sold is in conformance with the specications set forth
herein as determined by the assay methods described herein. Due to conditions of use,
technical errors or omissions, improper handling or storage beyond our control, Danisco
US Inc. hereby DISCLAIMS ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING
THE IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR
PURPOSE. Danisco US Inc. shall not be liable for any incidental, consequential, or special
damages resulting in any way from the furnishing, performance, or use of this product
literature or the product described herein.
Nothing contained herein shall be construed as a representation by Danisco US Inc.
that the use or resale of the product or processes described herein will not violate any
rules or regulations of any countries, regions, localities, etc. or infringe upon patents or
other intellectual property rights of third parties or that the recommendations and usage
suggestions described constitute any authorization or inducement to infringe any
such rights.
This document is subject to change without further notice.
REV1010 3155 DISTILLASE SSF 02
For more information contact:
USA and Canada
Rochester, New York
Telephone: 1-800-847-5311
Telefax: +1-585-256-5295
Europe, Africa and Middle East
Leiden, The Netherlands
Telephone: +31-71-5686-168
Telefax: +31-71-5686-169
Latin America
Buenos Aires, Argentina
Telephone: +54-11-4875-9500
Telefax: +54-11-4875-9529
Asia/Pacic
Singapore
Telephone: +65-6511-5600
Telefax: +65-6511-5666
Shanghai, P.R. China
Telephone: +86-21-2307-9588
Telefax: +86-21-2307-9599
Mumbai, India
Telephone: +91-22-2825-8713
Telefax: +91-22-2825-8733
Web Address
www.genencor.com

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