i AS 5013.

25—2009
ISO 21567:2004

Australian Standard®
Food microbiology
AS 5013.25—2009

Method 25: Microbiology of food and animal

feeding stuffs—Horizontal method for the
detection of Shigella spp.

PREFACE
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This Standard was prepared by the Standards Australia Committee FT-024, Food Products,
and Constituted Subcommittee FT-024-01, Food Microbiology.
This Standard is identical with and reproduced from ISO 21567:2004, Microbiology of food
and animal feeding stuffs—Horizontal method for the detection of Shigella spp.
ISO 21567:2004 was confirmed on 7 May 2008.
The objective of this Standard is to specify a horizontal method for the detection of Shigella
spp.
In Annex B, Composition and preparation of culture media and reagents, B.1.1 Shigella
broth, B.1.1.1 Composition, change:
‘Potassium hydrogen phosphate (anhydrous) … 2,0 g’ to ‘Dipotassium hydrogen phosphate
(K2 HPO 4) … 2,0 g’.
As this Standard is reproduced from an international standard, the following applies:
(a) In the source text ‘this International Standard’ should read ‘this Australian Standard’.
(b) A full point substitutes for a comma when referring to a decimal marker.
(c) Substitute ‘mL’ for ‘ml’ wherever it appears.
References to International Standards should be replaced by references to Australian
Standards, as follows:
Reference to International Standard Australian Standard
ISO AS
6887 Microbiology of food and animal 5013 Food microbiology
feeding stuffs—Preparation of test 5013.11.1 Method 11.1: Microbiology of
samples, initial suspension and food and animal feeding stuffs—
decimal dilutions for Preparation of test samples
microbiological examinations initial suspension and decimal
6887-1 Part 1: General rules for the dilutions for microbiological
preparation of the initial suspension examination—General rules for
and decimal dilutions the preparation of the initial
suspension and decimal
dilutions

www.standards.org.au
 iiii

ISO AS
6887-2 Part 2: Specific rules for the 5013.11.2 Method 11.2: Microbiology of
preparation of meat and meat food and animal feeding stuffs—
products Preparation of test samples
initial suspension and decimal
dilutions for microbiological
examination—Specific rules for
the preparation of meat and meat
products
6887-3 Part 3: Specific rules for the 5013.11.3 Method 11.3: Microbiology of
preparation of fish and fishery food and animal feeding stuffs—
products Preparation of test samples
initial suspension and decimal
dilutions for microbiological
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examination—Specific rules for

the preparation of fish and
fishery products
6887-4 Part 4: Specific rules for the 5013.11.4 Method 11.4: Microbiology of
preparation of products other than food and animal feeding stuffs—
milk and milk products, meat and Preparation of test samples
meat products and fish and fishery initial suspension and decimal
products dilutions for microbiological
examination—Specific rules for
the preparation of products other
than milk and milk products,
meat and meat products and fish
and fishery products
7218 Microbiology of food and animal 5013.14 Method 14: Microbiology of
feeding stuffs—General guidance food and animal feeding stuffs—
for microbiological examination General rules for
microbiological examinations
8261 Milk and milk products—General 5013.17 Method 17: Milk and milk
guidance for the preparation of test products—General guidance for
samples, initial suspensions and the preparation of test samples,
decimal dilutions for initial suspensions and decimal
microbiological examination dilutions for microbiological
examinations
Only international references that have been adopted as Australian Standards have been
listed.
The laboratory should have a clearly defined quality control system to ensure that the
apparatus, culture media, reagents and technique are suitable for the test. The use of
positive controls is part of this system.
The term ‘normative’ has been used in this Standard to define the application of the annex
to which it applies. A ‘normative’ annex is an integral part of a Standard.
 iii
iii

Introduction INTRODUCTION

Because of the large variety of food and feed products, this horizontal method may not be appropriate in every
detail for certain products. In this case, different methods which are specific to these products may be used if
absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply this
horizontal method as far as possible.

When this International Standard is next reviewed, account will be taken of all information then available
regarding the extent to which this horizontal method has been followed and the reasons for deviations from
this method in the case of particular products.

The harmonization of test methods cannot be immediate and, for certain groups of products, International
Standards and/or national standards may already exist that do not comply with this horizontal method. It is
hoped that when such standards are reviewed they will be changed to comply with this International Standard
so that eventually the only remaining departures from this horizontal method will be those necessary for
well-established technical reasons.
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© ISO 2004 – All rights reserved v

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iv
iv

NOTES
 1
1
Microbiology of food and animal feeding stuffs — Horizontal
method for the detection of Shigella spp.

WARNING — In order to safeguard the health of laboratory personnel, it is essential that the whole of
this method is only carried out by skilled personnel using good laboratory practices and preferably
working in a containment facility. Relevant national Health and Safety Regulations relating to this
organism shall be adhered to. Care shall be taken in the disposal of all infectious materials.

1 Scope
This International Standard specifies a horizontal method for the detection of Shigella species.

Subject to the limitations discussed in the Introduction, this International Standard is applicable to
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 products intended for human consumption and the feeding of animals, and

 environmental samples in the area of food production and food handling.

2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.

ISO 6887-1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial
suspension and decimal dilutions

ISO 6887-2, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and
meat products

ISO 6887-3, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and
fishery products

ISO 6887-4, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of products
other than milk and milk products, meat and meat products, and fish and fishery products

ISO 7218:1996, Microbiology of food and animal feeding stuffs — General rules for microbiological
examinations

ISO 8261, Milk and milk products — General guidance for the preparation of test samples, initial suspensions
and decimal dilutions for microbiological examination

ISO/TS 11133-1, Microbiology of food and animal feeding stuffs — Guidelines on preparation and production
of culture media — Part 1: General guidelines on quality assurance of culture media in the laboratory

ISO/TS 11133-2, Microbiology of food and animal feeding stuffs — Guidelines on preparation and production
of culture media — Part 2: Practical guidelines on performance testing of culture media

© ISO 2004 – All rights reserved 1

www.standards.org.au © Standards Australia

 This is a free preview. Purchase the entire publication at the link below:

AS 5013.25-2009, Food microbiology Microbiology

of food and animal feeding stuffs - Horizontal
method for the detection of Shigella spp.
This is a free 5 page sample. Access the full version at http://infostore.saiglobal.com.

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