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Spectral Analysis

This document outlines various analytical techniques used for spectral analysis and chromatography. It discusses 1) ultraviolet-visible spectroscopy, infrared spectroscopy, and nuclear magnetic resonance spectroscopy, noting factors that influence their spectral characteristics. 2) Chromatography techniques including column, flash, vacuum liquid, HPLC, TLC, and various other types, as well as separation mechanisms. 3) Additional analytical techniques like thermal methods, electrophoresis, crystallography and spectrofluorimetry. Key parameters, applications, and references are provided.

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Raghuveer Bheemshetty
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© Attribution Non-Commercial (BY-NC)
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33 views

Spectral Analysis

This document outlines various analytical techniques used for spectral analysis and chromatography. It discusses 1) ultraviolet-visible spectroscopy, infrared spectroscopy, and nuclear magnetic resonance spectroscopy, noting factors that influence their spectral characteristics. 2) Chromatography techniques including column, flash, vacuum liquid, HPLC, TLC, and various other types, as well as separation mechanisms. 3) Additional analytical techniques like thermal methods, electrophoresis, crystallography and spectrofluorimetry. Key parameters, applications, and references are provided.

Uploaded by

Raghuveer Bheemshetty
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as PDF, TXT or read online on Scribd
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SPECTRAL ANALYSIS

1. ultra violet & visible spectroscopy :


basic principle ,characteristics region of spectrum, energy levels & selection rules,
woodward – fieser , influence of substituent ,ring size & strain
on spectral characteristics , solvent effect non-conjugated interaction ,spectral correlation
with structure , uv- values .
(ref.pavia )
2. infrared spectroscopy :
characteristics region of spectrum , influence of substituents , ring size , hydrogen bond ,
vibrational coupling & field effect on frequency . spectral interpretation , ir value.
.(ref.pavia )
3. Nulear magnetic resonance spectrum :
Magnetice nuclei , chemical shift & shielding , relaxation ,processes ., chemical &^
magenetce non equivanence , local dinagnetce shielding , magnetice anisotropy , spin –
spin splitting , pascal triangle , coupling constant .
.(ref.pavia )
Application of raman spectra nicely.

c)Analytical techniques
1. chromatography : ( only go for basics)
general principles , classification , normal & reversed phase , bonded phase,
sepration mechanisms .
types: a. column chroma tography .
b. flash chromatography .
c. vaccum liquid chromatography .
d. hplc
e. tlc, hptlc, oplc (over pressure layer chromatography )
f. centrifugal chromatography .
g. counter – current chromatography .
h. droplet – counter current chromatography .
i. ion exchange , affinity , size exclusion & ion pair chromatography
perfusion , fast protein liquid chromatography, supercritical chrmo.
j. gc, gc-ms, lc- ms .
2. Thermal techniques :dsc, dta, tga .ord , cd (Very nicely) .
3. Electrophoresis : capillary, gel .
4. other : crystallography , spectrofluorimetry

Metries like potentiometry, polarimetry only imp equations and reference standard and imp
applications if any…

Refer standard books like pavia, kemp, skoog…

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